Joseph Clark

Joseph F Clark

Professor

Medical Sciences Building

7110

COM Neurology and Rehabilitation - 0536

Medical Sciences Building
231 Albert Sabin Way, Room 1055B MSB
Cincinnati, Ohio 45267-0536

Medical Sciences Building
Room 7112
Cincinnati, Ohio 45267

Education

Bachelor's Degree, Susquehanna University

Athletic Training Certification (ATC), Susquehanna University

Master's Degree, Michigan State University

Doctoral Degree, Michigan State University

Fellowship, Centre University de Paris Sud

Fellowship, University of Oxford (Research)

Positions and Work Experience

2006 - Professor of Neurology, Associate Professor, Department of Neurology, University of Cincinnati Associate Professor, Department of Neurology, Children’s’ Hospital Adjunct Associate Professor; Department of Molecular and Cellular Physiology Faculty Member; Neuroscience Program Faculty Member/Executive Committee; Physician Scientist Training Program Cincinnati OH Previous Posts: Graduate Student and Teaching Assistant: Department of Physiology 1986-1990 Michigan State University, East Lansing MI. 1990-1991 Postdoctoral Fellow, Fogarty Fellowship: Centre University de Paris Sud, INSERM U-241, Bat. 443, Orsay France. 1992-1995 Junior Research Fellow, Linacre College, University of Oxford, 1991-1997 MRC Non-Clinical Research Scientist, Department of Biochemistry, Oxford University, England. 1995-1999. Lecturer in Medicine Worcester College, Oxford University. 1996 - 2000. Lecturer in Biochemistry St. Anne’s College, Oxford University. 1997 - 2000 Group Leader, Vascular Smooth Muscle Research Group, MRC Collaborative Centre. , University of Cincinnati, Cincinnati, OH

Research and Practice Interests

Causes of cerebral vasospasm, complications following stroke or hemorrhaging into and around the brain, mechanisms of cerebral vascular disease, vascular metabolism, and creatine metabolism.

Research Support

Grant: #U54 EB007954 Investigators:Fred Beyette and Joseph Clark 10-01-2007 -09-30-2012 NIH/NIBIB Point of Care - Center for Emerging Neurotechnologies (POC-CENT) Role:Co-PI Active Type:Grant Level:National

Grant: #U54 EB007954 Investigators:Joseph Clark 10-01-2007 -09-30-2012 NIH/NIBIB Point of Care - Center for Emerging Neurotechnologies (POC-CENT) Devices Role:PI Active Type:Grant Level:National

Grant: #RO1 NS050569 Investigators:Joseph Clark, Gail Pyne-Geithman and Kenneth Wagner 07-15-2005 -05-31-2012 NIH/NINDS Bilirubin oxidation and intracerebral hemorrhage Role:PI Completed Type:Grant Level:National

Grant: #R01 NS057367 Investigators:Aigang Lu, Joseph Clark 04-01-2008 -03-31-2012 NIH/NINDS Reperfusion & Postischemic Hemorrhage in Brain Role:Co-PI Active Type:Grant Level:National

Grant: #R01 NS049428 Investigators:Gail Pyne-Geithman, Joseph Clark 02-04-2005 -01-31-2011 NIH/NINDS Cerebral Vasospasm After Subarachnoid Hemorrhage. Role:Co-PI Completed Type:Grant Level:National

Grant: #SRS 000583 Investigators:Clark, Joseph; Kempisty, Suzanne 12-01-2006 -11-30-2008 University of Cincinnati Neuroscience Institute Biomarkers for Subarachnoid Hemorrhage Induced Cerebral Vasospasm Role:PI $22,186.00 Completed

Grant: #SRS 000584 Investigators:Clark, Joseph; Lu, Aigang 12-01-2006 -11-30-2008 University of Cincinnati Neuroscience Institute Can Micro MRI Predict Hemorrhagic Transformation Post Ischemic Reperfusion? Role:Collaborator $24,164.00 Completed

Grant: #SRS 001904 Investigators:Clark, Joseph 03-15-2006 -04-14-2008 Nuvelo, Inc. Alfimeperase Safety Following Ischemia Reperfusion Role:PI $225,821.00 Completed

Investigators:Clark, Joseph 06-01-2003 -05-31-2007 National Institute of Neurological Disorders and Stroke Biochemical Mechanisms of Cerebral Vasospasm Role:PI $757,836.00 Closed Level:Federal

Grant: #R01 BS49172 Investigators:Clark, Joseph 07-15-2004 -04-30-2008 National Institute of Neurological Disorders and Stroke Creatine Transport Across the Blood Brain Barrier Role:PI $451,543.00 Completed

Grant: #R01 NS050569 Investigators:Clark, Joseph 07-15-2005 -05-31-2011 National Institute of Neurological Disorders and Stroke Bilirubin Oxidation and Intracerebral Hemorrhage Role:PI $1,788,072.00 Completed

Grant: #5-R01-HL-67186-03-A0-S0-E0 Investigators:Clark, Joseph 03-06-2001 -01-31-2004 National Heart, Lung and Blood Institute Biochemical Mechanisms of Vasospasms Role:PI $1,140,080.00 Closed Level:Federal

Grant: #0150989B-02-A0-S0-E0 Investigators:Clark, Joseph 07-01-2001 -06-30-2003 American Heart Association - Southwest Chapter Vascular Proliferation Associated with Subarachnoid Hemorrhage Induced Cerebral Vasospasm Role:PI $110,000.00 Closed Level:Private Non-Profit

Grant: #5-R21-NS-42697-02-A0-S0-E0 Investigators:Clark, Joseph 03-01-2002 -02-28-2005 National Institute of Neurological Disorders and Stroke Developing Antibodies to Diagnose Cerebral Vasospasm Role:PI $363,376.00 Closed Level:Federal

Grant: #U54 EB007954 Investigators:Air, Dorothy; Beyette, Fred; Bishop, Paul; Broderick, Joseph; Clark, Joseph; Haridas, Balakrishna; Helmicki, Arthur; Jauch, Edward; Kanter, Daniel; Pancioli, Arthur; Papautsky, Ian; Privitera, Mary Beth; Shutter, Lori; Steckl, Andrew; Todd, Anita; Tomsick, Thomas; Wilsey, Philip 09-30-2007 -06-30-2012 National Institute of Biomedical Imaging and Bioengineering Point of Care-Care Center for Emerging Neurotechnologies Role:Collaborator $9,416,342.00 Active Level:Federal

Grant: #R01 NS057367 Investigators:Clark, Joseph; Lu, Aigang; Pyne-Geithman, Gail Jean; Tomsick, Thomas; Wagner, Kenneth 04-01-2008 -03-31-2013 National Institute of Neurological Disorders and Stroke Reperfusion and Postischemic Hemorrhage in Brain Role:Collaborator $1,354,763.00 Active Level:Federal

Grant: #SRS 005588 Investigators:Clark, Joseph; Pyne-Geithman, Gail Jean 04-01-2008 -12-31-2010 Neuro Resource Group, Inc. Analysis of Lymphocyte Oxidative Metabolism with NRG Role:PI $18,870.00 Completed

Grant: #P50 NS044283 Investigators:Broderick, Joseph; Clark, Joseph; Flaherty, Matthew; Holland, Christy; Jauch, Edward; Lindsell, Christopher; Lu, Aigang; Pancioli, Arthur; Pyne-Geithman, Gail Jean; Shaw, George 08-01-2008 -04-30-2013 National Institute of Neurological Disorders and Stroke Recanalization Therapies and Markers of Stroke Outcome Role:Collaborator $7,150,853.00 Active Level:Federal

Grant: #NRG 20-1139829 Investigators:Clark, Joseph; Pyne-Geithman, Gail Jean 03-14-2011 -05-09-2011 Neuro Resource Group, Inc. Endogenous Pain Sensation and Sensitivity Molecule Measurements in Plasma of Humans: TENS vs. InterX in Four Subjects Role:Collaborator $16,900.00 Active Level:Industry

Grant: #S10OD011944 Investigators:Adler, Caleb; Clark, Joseph; Ho, Shuk-Mei; Howarth, Jack; Komoroski, Richard; Lu, Aigang; Pyne-Geithman, Gail Jean; Rosevear, Paul; Strakowski, Stephen 04-02-2012 -03-31-2013 Office of the Director, National Institutes of Health Bruker Avance III Wide-Bore (89-mm, 9.4T), Vertical-Bore 400-Mhz NMR Spectrometer Role:Collaborator $595,000.00 Active Level:Federal

Publications

Peer Reviewed Publications

Loftspring,M. C., McDole,J., Lu,A., Clark,J. F., Johnson,A. J. (2009. ) Intracerebral hemorrhage leads to infiltration of several leukocyte populations with concomitant pathophysiological changes .Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, , 29 (1 ) ,137 -143

Ellis,J., Del Castillo,E., Montes Bayon,M., Grimm,R., Clark,J. F., Pyne-Geithman,G., Wilbur,S., Caruso,J. A. (2008. ) A preliminary study of metalloproteins in CSF by CapLC-ICPMS and NanoLC-CHIP/ITMS .Journal of proteome research, , 7 (9 ) ,3747 -3754

Wurster,W. L., Pyne-Geithman,G. J., Peat,I. R., Clark,J. F. (2008. ) Bilirubin oxidation products (BOXes): synthesis, stability and chemical characteristics .Acta neurochirurgica.Supplement, , 104 ,43 -50

Clark,J. F., Loftspring,M., Wurster,W. L., Beiler,S., Beiler,C., Wagner,K. R., Pyne-Geithman,G. J. (2008. ) Bilirubin oxidation products, oxidative stress, and intracerebral hemorrhage .Acta neurochirurgica.Supplement, , 105 ,7 -12

Clark,J. F., Loftspring,M., Wurster,W. L., Pyne-Geithman,G. J. (2008. ) Chemical and biochemical oxidations in spinal fluid after subarachnoid hemorrhage .Frontiers in bioscience : a journal and virtual library, , 13 ,1806 -1812

Brewster,L. M., van Bree,S., Reijneveld,J. C., Notermans,N. C., Verschuren,W. M., Clark,J. F., van Montfrans,G. A., de Visser,M. (2008. ) Hypertension risk in idiopathic hyperCKemia .Journal of neurology, , 255 (1 ) ,11 -15

Pyne-Geithman,G. J., Nair,S. G., Caudell,D. N., Clark,J. F. (2008. ) PKC and Rho in vascular smooth muscle: activation by BOXes and SAH CSF .Frontiers in bioscience : a journal and virtual library, , 13 ,1526 -1534

Lu,A., Clark,J. F., Broderick,J. P., Pyne-Geithman,G. J., Wagner,K. R., Ran,R., Khatri,P., Tomsick,T., Sharp,F. R. (2008. ) Reperfusion activates metalloproteinases that contribute to neurovascular injury .Experimental neurology, , 210 (2 ) ,549 -559

Ellis,J., Grimm,R., Clark,J. F., Pyne-Gaithman,G., Wilbur,S., Caruso,J. A. (2008. ) Studying protein phosphorylation in low MW CSF fractions with capLC-ICPMS and nanoLC-CHIP-ITMS for identification of phosphoproteins .Journal of proteome research, , 7 (11 ) ,4736 -4742

Loftspring,M. C., Clark,J. F., Wagner,K. R. (2007. ) A novel duplex ELISA method for quantitation of plasma proteins in areas of brain edema .Brain research, , 1162 ,130 -132

Loftspring,M. C., Wurster,W. L., Pyne-Geithman,G. J., Clark,J. F. (2007. ) An in vitro model of aneurysmal subarachnoid hemorrhage: oxidation of unconjugated bilirubin by cytochrome oxidase .Journal of neurochemistry, , 102 (6 ) ,1990 -1995

Bhadri,P. R., Salgaonkar,V. A., Pyne-Geithman,G. J., Caffery,J. J.,Jr, Shukla,R., Beyette,F. R.,Jr, Clark,J. F. (2007. ) Spectrophotometric quantification of bilirubin in hemorrhagic spinal fluid using an innovative algorithm .Medicinal chemistry (Shariqah (United Arab Emirates)), , 3 (1 ) ,21 -27

Clark,J. F., Sharp,F. R. (2006. ) Bilirubin oxidation products (BOXes) and their role in cerebral vasospasm after subarachnoid hemorrhage .Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, , 26 (10 ) ,1223 -1233

Brewster,L. M., Mairuhu,G., Bindraban,N. R., Koopmans,R. P., Clark,J. F., van Montfrans,G. A. (2006. ) Creatine kinase activity is associated with blood pressure .Circulation, , 114 (19 ) ,2034 -2039

Field,M. L., Khan,O., Abbaraju,J., Clark,J. F. (2006. ) Functional compartmentation of glycogen phosphorylase with creatine kinase and Ca2+ ATPase in skeletal muscle .Journal of theoretical biology, , 238 (2 ) ,257 -268

Clark,J. F., Doepke,A., Filosa,J. A., Wardle,R. L., Lu,A., Meeker,T. J., Pyne-Geithman,G. J. (2006. ) N-acetylaspartate as a reservoir for glutamate .Medical hypotheses, , 67 (3 ) ,506 -512

Pyne-Geithman,G. J., Morgan,C. J., Wagner,K., Dulaney,E. M., Carrozzella,J., Kanter,D. S., Zuccarello,M., Clark,J. F. (2005. ) Bilirubin production and oxidation in CSF of patients with cerebral vasospasm after subarachnoid hemorrhage .Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, , 25 (8 ) ,1070 -1077

Newmeyer,A., Cecil,K. M., Schapiro,M., Clark,J. F., Degrauw,T. J. (2005. ) Incidence of brain creatine transporter deficiency in males with developmental delay referred for brain magnetic resonance imaging .Journal of developmental and behavioral pediatrics : JDBP, , 26 (4 ) ,276 -282

Clark,J. F., Pyne-Geithman,G. (2005. ) Vascular smooth muscle function: The physiology and pathology of vasoconstriction .Pathophysiology : the official journal of the International Society for Pathophysiology / ISP, , 12 (1 ) ,35 -45

Biehle,S. J., Carrozzella,J., Shukla,R., Popplewell,J., Swann,M., Freeman,N., Clark,J. F. (2004. ) Apolipoprotein E isoprotein-specific interactions with tissue plasminogen activator .Biochimica et biophysica acta, , 1689 (3 ) ,244 -251

Morgan,C. J., Pyne-Geithman,G. J., Jauch,E. C., Shukla,R., Wagner,K. R., Clark,J. F., Zuccarello,M. (2004. ) Bilirubin as a cerebrospinal fluid marker of sentinel subarachnoid hemorrhage: a preliminary report in pigs .Journal of neurosurgery, , 101 (6 ) ,1026 -1029

Lyons,M. A., Shukla,R., Zhang,K., Pyne,G. J., Singh,M., Biehle,S. J., Clark,J. F. (2004. ) Increase of metabolic activity and disruption of normal contractile protein distribution by bilirubin oxidation products in vascular smooth-muscle cells .Journal of neurosurgery, , 100 (3 ) ,505 -511

Jain,S., Jayasimhulu,K., Clark,J. F. (2004. ) Metabolomic analysis of molecular species of phospholipids from normotensive and preeclamptic human placenta electrospray ionization mass spectrometry .Frontiers in bioscience : a journal and virtual library, , 9 ,3167 -3175

Pyne-Geithman,G. J., deGrauw,T. J., Cecil,K. M., Chuck,G., Lyons,M. A., Ishida,Y., Clark,J. F. (2004. ) Presence of normal creatine in the muscle of a patient with a mutation in the creatine transporter: a case study .Molecular and cellular biochemistry, , 262 (1-2 ) ,35 -39

Lu,A., Tang,Y., Ran,R., Clark,J. F., Aronow,B. J., Sharp,F. R. (2003. ) Genomics of the periinfarction cortex after focal cerebral ischemia .Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, , 23 (7 ) ,786 -810

Wagner,K. R., Sharp,F. R., Ardizzone,T. D., Lu,A., Clark,J. F. (2003. ) Heme and iron metabolism: role in cerebral hemorrhage .Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, , 23 (6 ) ,629 -652

Cecil,K. M., DeGrauw,T. J., Salomons,G. S., Jakobs,C., Egelhoff,J. C., Clark,J. F. (2003. ) Magnetic resonance spectroscopy in a 9-day-old heterozygous female child with creatine transporter deficiency .Journal of computer assisted tomography, , 27 (1 ) ,44 -47

Nakayama,S., Nomura,H., Smith,L. M., Clark,J. F., Uetani,T., Matsubara,T. (2003. ) Mechanisms for monovalent cation-dependent depletion of intracellular Mg2+:Na(+)-independent Mg2+ pathways in guinea-pig smooth muscle .The Journal of physiology, , 551 (Pt 3 ) ,843 -853

Pyne,G. J., Cadoux-Hudson,T. A., Clark,J. F. (2003. ) Platelets play an essential role in the aetiology of cerebral vasospasm after subarachnoid haemorrhage .Medical hypotheses, , 60 (4 ) ,525 -530

Nakayama,S., Clark,J. F. (2003. ) Smooth muscle and NMR review: an overview of smooth muscle metabolism .Molecular and cellular biochemistry, , 244 (1-2 ) ,17 -30

Kreider,R. B., Burke,E. R., Clark,J. F., Earnest,C., Greenwood,M., Harris,R., Kalman,D. S., Kleiner,S. M., Serrano,E., Volek,J. S., Ziegenfuss,T. N., Willoughby,D. S. (2003. ) The neurosurgeon in sport: awareness of the risks of heatstroke and dietary supplements .Neurosurgery, , 52 (1 ) ,252 -5; author reply 255-7

Clark,J. F., Huri,D. A., Carrozzella,J., Jauch,E. C., Mehta,P., Heaton,D., Biehle,S. J., Broderick,J. P. (2002. ) Isoforms of apolipoprotein E can modulate tPA-induced clot lysis in vitro .Frontiers in bioscience : a journal and virtual library, , 7 ,a163 -8

Clark,J. F., Reilly,M., Sharp,F. R. (2002. ) Oxidation of bilirubin produces compounds that cause prolonged vasospasm of rat cerebral vessels: a contributor to subarachnoid hemorrhage-induced vasospasm .Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, , 22 (4 ) ,472 -478

Nakayama,S., Nomura,H., Smith,L. M., Clark,J. F. (2002. ) Simultaneous estimation of intracellular free Mg2+ and pH by use of a new pH-dependent dissociation constant of MgATP .The Japanese journal of physiology, , 52 (3 ) ,323 -326

Pyne,G. J., Cadoux-Hudson,T. A., Clark,J. F. (2001. ) Cerebrospinal fluid from subarachnoid haemorrhage patients causes excessive oxidative metabolism compared to vascular smooth muscle force generation .Acta Neurochirurgica, , 143 (1 ) ,59 -62; discussion 62-3

Clark,J. F., Pyne,G. J., Choutka,J., Carrozzella,J. A., Khoury,J., Broderick,J. P., Cadoux-Hudson,T. A. (2001. ) In vitro therapy with dobutamine, isoprenaline and sodium nitroprusside protects vascular smooth muscle metabolism from subarachnoid haemorrhage induced cerebral vasospasm .Acta Neurochirurgica, , 143 (7 ) ,721 -728

Pyne,G. J., Cadoux-Hudson,T. A., Clark,J. F. (2001. ) Magnesium protection against in vitro cerebral vasospasm after subarachnoid haemorrhage .British journal of neurosurgery, , 15 (5 ) ,409 -415

Cadoux-Hudson,T. A., Pyne,G. J., Domingo,Z., Clark,J. F. (2001. ) The stimulation of vascular smooth muscle oxidative metabolism by CSF from subarachnoid haemorrhage patients increases with Fisher and WFNS grades .Acta Neurochirurgica, , 143 (1 ) ,65 -72

Clark,J. F., Matsumoto,T., Nakayama,S. (2000. ) Intact smooth muscle metabolism: its responses to cyanide poisoning and pyruvate stimulation .Frontiers in bioscience : a journal and virtual library, , 5 ,A18 -23

Brewster,L. M., Clark,J. F., van Montfrans,G. A. (2000. ) Is greater tissue activity of creatine kinase the genetic factor increasing hypertension risk in black people of sub-Saharan African descent? .Journal of hypertension, , 18 (11 ) ,1537 -1544

Kranc,K. R., Pyne,G. J., Tao,L., Claridge,T. D., Harris,D. A., Cadoux-Hudson,T. A., Turnbull,J. J., Schofield,C. J., Clark,J. F. (2000. ) Oxidative degradation of bilirubin produces vasoactive compounds .European journal of biochemistry / FEBS, , 267 (24 ) ,7094 -7101

Clark,J. F., Radda,G. K., Boehm,E. A. (2000. ) The effects of anti-hypertensive therapy on the structural, mechanical and metabolic properties of the rat aorta .Journal of muscle research and cell motility, , 21 (3 ) ,255 -267

Thomson,N. F., Thornton,S., Clark,J. F. (2000. ) The effects of placental extracts from normotensive and preeclamptic women on vasoconstriction and oxidative metabolism .American Journal of Obstetrics and Gynecology, , 183 (1 ) ,206 -210

Pyne,G. J., Cadoux-Hudson,T. A., Clark,J. F. (2000. ) The presence of an extractable substance in the CSF of humans with cerebral vasospasm after subarachnoid haemorrhage that correlates with phosphatase inhibition .Biochimica et biophysica acta, , 1474 (3 ) ,283 -290

Kunz,W. S., Kuznetsov,A. V., Clark,J. F., Tracey,I., Elger,C. E. (1999. ) Metabolic consequences of the cytochrome c oxidase deficiency in brain of copper-deficient Mo(vbr) mice .Journal of neurochemistry, , 72 (4 ) ,1580 -1585

Clark,J. F. (1998. ) Creatine: a review of its nutritional applications in sport .Nutrition (Burbank, Los Angeles County, Calif.), , 14 (3 ) ,322 -324

Kemp,G. J., Manners,D. N., Clark,J. F., Bastin,M. E., Radda,G. K. (1998. ) Theoretical modelling of some spatial and temporal aspects of the mitochondrion/creatine kinase/myofibril system in muscle .Molecular and cellular biochemistry, , 184 (1-2 ) ,249 -289

Bearchell,M. C., Redman,C. W., Pyne,G. J., Cadoux-Hudson,T., Clark,J. F. (1998. ) Vascular smooth muscle oxygen consumption is reversibly stimulated by sera from women with preeclampsia .American Journal of Obstetrics and Gynecology, , 179 (6 Pt 1 ) ,1534 -1538

Kemp,G. J., Manners,D. N., Clark,J. F., Bastin,M. E., Radda,G. K. (1997. ) A theoretical model of some spatial and temporal aspects of the mitochondrion creatine kinase myofibril system in muscle .Molecular and cellular biochemistry, , 174 (1-2 ) ,29 -32

Clark,J. F., Kuznetsov,A. V., Radda,G. K. (1997. ) ADP-regenerating enzyme systems in mitochondria of guinea pig myometrium and heart .The American Journal of Physiology, , 272 (2 Pt 1 ) ,C399 -404

Nakayama,S., Chihara,S., Clark,J. F., Huang,S. M., Horiuchi,T., Tomita,T. (1997. ) Consequences of metabolic inhibition in smooth muscle isolated from guinea-pig stomach .The Journal of physiology, , 505 ( Pt 1) (Pt 1 ) ,229 -240

Clark,J. F. (1997. ) Creatine and Phosphocreatine: A Review of Their Use in Exercise and Sport .Journal of athletic training, , 32 (1 ) ,45 -51

Field,M. L., Clark,J. F. (1997. ) Inappropriate ubiquitin conjugation: a proposed mechanism contributing to heart failure .Cardiovascular research, , 33 (1 ) ,8 -12

Leighton,B., Sanderson,A. L., Young,M. E., Radda,G. K., Boehm,E. A., Clark,J. F. (1996. ) Effects of treatment of spontaneously hypertensive rats with the angiotensin-converting enzyme inhibitor trandolapril and the calcium antagonist verapamil on the sensitivity of glucose metabolism to insulin in rat soleus muscle in vitro .Diabetes, , 45 Suppl 1 ,S120 -4

Kuznetsov,A. V., Clark,J. F., Winkler,K., Kunz,W. S. (1996. ) Increase of flux control of cytochrome c oxidase in copper-deficient mottled brindled mice .The Journal of biological chemistry, , 271 (1 ) ,283 -288

Boehm,E. A., Radda,G. K., Tomlin,H., Clark,J. F. (1996. ) The utilisation of creatine and its analogues by cytosolic and mitochondrial creatine kinase .Biochimica et biophysica acta, , 1274 (3 ) ,119 -128

Boehm,E. A., Clark,J. F., Radda,G. K. (1995. ) Metabolite utilization and compartmentation in porcine carotid artery: a study using beta-guanidinopropionic acid .The American Journal of Physiology, , 268 (3 Pt 1 ) ,C628 -35

Clark,J. F., Dillon,P. F. (1995. ) Phosphocreatine and creatine kinase in energetic metabolism of the porcine carotid artery .Journal of vascular research, , 32 (1 ) ,24 -30

Clark,J. F., Kemp,G. J., Radda,G. K. (1995. ) The creatine kinase equilibrium, free [ADP] and myosin ATPase in vascular smooth muscle cross-bridges .Journal of theoretical biology, , 173 (2 ) ,207 -211

Clark,J. F., Khuchua,Z., Kuznetsov,A. V., Vassil'eva,E., Boehm,E., Radda,G. K., Saks,V. (1994. ) Actions of the creatine analogue beta-guanidinopropionic acid on rat heart mitochondria .The Biochemical journal, , 300 ( Pt 1) (Pt 1 ) ,211 -216

Field,M. L., Clark,J. F., Henderson,C., Seymour,A. M., Radda,G. K. (1994. ) Alterations in the myocardial creatine kinase system during chronic anaemic hypoxia .Cardiovascular research, , 28 (1 ) ,86 -91

Clark,J. F., Khuchua,Z., Boehm,E., Ventura-Clapier,R. (1994. ) Creatine kinase activity associated with the contractile proteins of the guinea-pig carotid artery .Journal of muscle research and cell motility, , 15 (4 ) ,432 -439

Clark,J. F., Kuznetsov,A. V., Khuchua,Z., Veksler,V., Ventura-Clapier,R., Saks,V. (1994. ) Creatine kinase function in mitochondria isolated from gravid and non-gravid guinea-pig uteri .FEBS letters, , 347 (2-3 ) ,147 -151

Clark,J. F. (1994. ) The creatine kinase system in smooth muscle .Molecular and cellular biochemistry, , 133-134 ,221 -232

Clark,J. F., Khuchua,Z., Kuznetsov,A., Saks,V. A., Ventura-Clapier,R. (1993. ) Compartmentation of creatine kinase isoenzymes in myometrium of gravid guinea-pig .The Journal of physiology, , 466 ,553 -572

Clark,J. F., Khuchua,Z., Ventura-Clapier,R. (1992. ) Creatine kinase binding and possible role in chemically skinned guinea-pig taenia coli .Biochimica et biophysica acta, , 1100 (2 ) ,137 -145

Khuchua,Z. A., Vasiljeva,E. V., Clark,J. F., Korchazhkina,O. V., Branishte,T., Kapelko,V. I., Kuznetsov,A. V., Ventura-Clapier,R., Steinschneider,A. Ya, Lakomkin,V. L. (1992. ) The creatine kinase system and cardiomyopathy .The American Journal of Cardiovascular Pathology, , 4 (3 ) ,223 -234

Clark,J. F., Harris,G. I., Dillon,P. F. (1991. ) Multisite saturation transfer using DANTE and continuous wave .Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, , 17 (1 ) ,274 -278

Dillon,P. F., Clark,J. F. (1990. ) The theory of diazymes and functional coupling of pyruvate kinase and creatine kinase .Journal of theoretical biology, , 143 (2 ) ,275 -284

Dillon,P. F., Fisher,M. J., Adams,G. R., Clark,J. F. (1989. ) The use of 31P-NMR to study smooth muscle .Progress in clinical and biological research, , 315 ,439 -449

Brewster, L M; Taherzadeh, Z; Volger, S; Clark, J F; Rolf, T; Wolf, H; Vanbavel, E; van Montfrans, G A (2010. ) Ethnic differences in resistance artery contractility of normotensive pregnant women.American journal of physiology. Heart and circulatory physiology, , 299 (2 ) ,H431-6

McMullan, Jason T; Knight, William A; Clark, Joseph F; Beyette, Fred R; Pancioli, Arthur (2010. ) Time-critical neurological emergencies: the unfulfilled role for point-of-care testing.International journal of emergency medicine, , 3 (2 ) ,127-31

Kurosawa, Yuko; Lu, Aigang; Khatri, Pooja; Carrozzella, Janice A; Clark, Joseph F; Khoury, Jane; Tomsick, Thomas A (2010. ) Intra-arterial iodinated radiographic contrast material injection administration in a rat middle cerebral artery occlusion and reperfusion model: possible effects on intracerebral hemorrhage.Stroke; a journal of cerebral circulation, , 41 (5 ) ,1013-7

Hou, Shangwei; Xu, Rong; Clark, Joseph F; Wurster, William L; Heinemann, Stefan H; Hoshi, Toshinori (2010. ) Bilirubin oxidation end products directly alter K(+) channels important in the regulation of vascular tone.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, ,

Loftspring, Matthew C; Hansen, Craig; Clark, Joseph F (2010. ) A novel brain injury mechanism after intracerebral hemorrhage: the interaction between heme products and the immune system.Medical hypotheses, , 74 (1 ) ,63-6

Alla, Suresh K; Clark, Joseph F; Beyette, Fred R (2009. ) Signal processing system to extract serum bilirubin concentration from diffuse reflectance spectrum of human skin.Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, , 2009 ,1290-3

Zhang, Yaofang; Clark, Joseph F; Pyne-Geithman, Gail; Caruso, Joseph (2010. ) Metallomics study in CSF for putative biomarkers to predict cerebral vasospasm.Metallomics : integrated biometal science, , 2 (9 ) ,628-37

Kroening, Karolin K; Kuhlmann, Julia; Easter, Renee; Clark, Joseph F; Pyne-Geithman, Gail; Caruso, Joseph A (2010. ) Protein phosphorylation studies of cerebral spinal fluid for potential biomarker development.Metallomics : integrated biometal science, , 2 (5 ) ,334-41

Lu, Aigang; Clark, Joseph F; Ran, Ruiqiong; Pyne-Geithman, Gail; Wagner, Kenneth R; Millhorn, David E; Sharp, Frank R (2009. ) Down-regulation of interleukin 7 mRNA by hypoxia is calcium dependent.Neurological research, , 31 (5 ) ,545-9

Lu, Aigang; Clark, Joseph F; Broderick, Joseph P; Pyne-Geithman, Gail J; Wagner, Kenneth R; Khatri, Pooja; Tomsick, Thomas; Sharp, Frank R (2009. ) Mechanical reperfusion is associated with post-ischemic hemorrhage in rat brain.Experimental neurology, , 216 (2 ) ,407-12

Pyne-Geithman, Gail J; Caudell, Danielle N; Prakash, Porus; Clark, Joseph F (2009. ) Glutathione peroxidase and subarachnoid hemorrhage: implications for the role of oxidative stress in cerebral vasospasm.Neurological research, , 31 (2 ) ,195-9

Pyne-Geithman, Gail J; Caudell, Danielle N; Cooper, Matthew; Clark, Joseph F; Shutter, Lori A (2009. ) Dopamine D2-receptor-mediated increase in vascular and endothelial NOS activity ameliorates cerebral vasospasm after subarachnoid hemorrhage in vitro.Neurocritical care, , 10 (2 ) ,225-31

Clark, Joseph F; Ellis, James K; Bench, Johnny; Khoury, Jane; Graman, Pat (2012. ) High-performance vision training improves batting statistics for university of cincinnati baseball players.PloS one, , 7 (1 ) ,e29109

Butler, Joshua; Booher, Blaine; Bowman, Peggy S; Clark, Joseph F; Beyette, Fred R (2011. ) Improved quantification of CSF bilirubin in the presence of hemoglobin using least squares curve-fitting.Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, , 2011 ,1-4

Brewster, Lizzy M; Clark, Joseph F; van Montfrans, Gert A (2011. ) The Tromsø study confirms the association between creatine kinase and blood pressure.Journal of hypertension, , 29 (5 ) ,1019; author reply 1

Loftspring, Matthew C; Johnson, Holly L; Feng, Rui; Johnson, Aaron J; Clark, Joseph F (2011. ) Unconjugated bilirubin contributes to early inflammation and edema after intracerebral hemorrhage.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, , 31 (4 ) ,1133-42

Beyette, Fred R; Booher, Blaine; Drennan, James; Carraher, Lee; Butler, Josh; Bowman, Peggy S; Clark, Joseph F; Wilsey, Philip A (2011. ) Device for quantification of bilirubin in cerebral spinal fluid.IEEE transactions on bio-medical engineering, , 58 (3 ) ,773-6

Alla, Suresh K; Huddle, Adam; Butler, Joshua D; Bowman, Peggy S; Clark, Joseph F; Beyette, Fred R (2011. ) Point-of-care device for quantification of bilirubin in skin tissue.IEEE transactions on bio-medical engineering, , 58 (3 ) ,777-80

Skelton, Matthew R; Schaefer, Tori L; Graham, Devon L; Degrauw, Ton J; Clark, Joseph F; Williams, Michael T; Vorhees, Charles V (2011. ) Creatine transporter (CrT; Slc6a8) knockout mice as a model of human CrT deficiency.PloS one, , 6 (1 ) ,e16187

Clark, Joseph F; Harm, Amanda; Saffire, Ashlie; Biehle, Susan J; Lu, Aigang; Pyne-Geithman, Gail J (2011. ) Bilirubin oxidation products seen post subarachnoid hemorrhage have greater effects on aged rat brain compared to young.Acta neurochirurgica. Supplement, , 110 (Pt 1 ) ,157-62

Kroening, Karolin K; Kuhlmann, Julia; Easter, Renee; Clark, Joseph F; Pyne-Geithman, Gail; Caruso, Joseph A (2010. ) Protein phosphorylation studies of cerebral spinal fluid for potential biomarker development.Metallomics : integrated biometal science, , 2 (5 ) ,334-41

Beyette, Fred R; Booher, Blaine; Drennan, James; Carraher, Lee; Butler, Josh; Bowman, Peggy; Clark, Joseph F; Wilsey, Philip A (2010. ) Development of a point-of-care device for the quantification of bilirubin in cerebral spinal fluid.Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, , 2010 ,915-8

Alla, Suresh K; Huddle, Adam; Clark, Joseph F; Beyette, Fred R (2010. ) Signal processing system to quantify bilirubin in the jaundice clinical model spectra.Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference, , 2010 ,1356-9

Kurosawa, Yuko; Degrauw, Ton J; Lindquist, Diana M; Blanco, Victor M; Pyne-Geithman, Gail J; Daikoku, Takiko; Chambers, James B; Benoit, Stephen C; Clark, Joseph F (2012. ) Cyclocreatine treatment improves cognition in mice with creatine transporter deficiency.The Journal of clinical investigation, ,

Suofu, Y; Clark, J F; Broderick, J P; Kurosawa, Y; Wagner, K R; Lu, A (2012. ) Matrix metalloproteinase-2 or -9 deletions protect against hemorrhagic transformation during early stage of cerebral ischemia and reperfusion.Neuroscience, , 212 ,180-9

Brewster, Lizzy M; Coronel, Carmen M D; Sluiter, Willem; Clark, Joseph F; van Montfrans, Gert A (2012. ) Ethnic differences in tissue creatine kinase activity: an observational study.PloS one, , 7 (3 ) ,e32471

Honors and Awards

Fogarty International Research Award, Fogarty International Center: Affiliations: National Institutes of Health, USA, 1990. Franco-Soviet Biomedical Research Co-operation Project of Scientific Exchange, INSERM U-241 and The Laboratory of Car

Post Graduate Training and Education

1992-2000 Research Fellow and Lecturer, University of Oxford, , England

1990-1991 Postdoctoral Fellow, Centre University de Paris Sud, , Orsay, France

Keywords

Anatomy(MeSH A),Diseases(MeSH C),Chemicals and Drugs(MeSH D),Analytical, Diagnostic and Therapeutic Techniques and Equipment(MeSH E),Biological Sciences(MeSH G),Musculoskeletal System(MeSH A02),Cardiovascular System(MeSH A07),Nervous System(MeSH A08),Fluids and Secretions(MeSH A12),Animal Structures(MeSH A13),Musculoskeletal Diseases(MeSH C05),Nervous System Diseases(MeSH C10),Cardiovascular Diseases(MeSH C14),Nutritional and Metabolic Diseases(MeSH C18),Enzymes, Coenzymes, and Enzyme Inhibitors(MeSH D08),Amino Acids, Peptides, and Proteins(MeSH D12),Central Nervous System Agents(MeSH D15),Cardiovascular Agents(MeSH D18),Specialty Chemicals and Products(MeSH D26),Chemical Actions and Uses(MeSH D27),Diagnosis(MeSH E01),Therapeutics(MeSH E02),Biochemical Phenomena, Metabolism, and Nutrition(MeSH G06),Physiological Processes(MeSH G07),Circulatory and Respiratory Physiology(MeSH G09),Chemical and Pharmacologic Phenomena(MeSH G12), biliburin, vasospasm, stroke, creatine, vascular smooth muscle, oxidation, apolipoprotein, vascular, muscle

Contact Information

Research - Medical Sciences Building
231 Albert Sabin Way, Room 1055B MSB
Cincinnati  Ohio, 45267-0536
Phone: 513-558-7085
joseph.clark@uc.edu

Academic - Medical Sciences Building
Room 7112
Cincinnati  Ohio, 45267
Phone: 513-558-7009
joseph.clark@uc.edu