Daniel J Hassett , Ph.D.

Professor

Medical Sciences Building
231 Albert Sabin Way
Cincinnati, 45267
Phone 513-558-1154
Email daniel.hassett@uc.edu

Education

Ph.D.: SUNY-Syracuse 1990 (Microbial Pathogenesis)

Post Doc: University of North Carolina 1994

Research and Practice Interests

The broad objective of this investigation is to increase ourunderstanding of a pathogenic mechanism of Pseudomonas aeruginosa inchronic respiratory infections of cystic fibrosis patients within theairways of these patients, P aeruginosa must have mechanisms to surviveconsiderable oxidative stress in the forms of 1 phagocyte-mediatedoxygen radicals, 2 high P02 within this milieu, and 3 pyocyanin, atoxic redox compound produced by P aeruginosa. To combat this oxidativestress, P aeruginosa produces superoxide dismutases SODs, whichdisproportionate superoxide forming hydrogen peroxide, catalases whichdestroy hydrogen peroxide, and alginate a scavenger of extra-cellularoxygen radicals. We have recently shown that the alginate regulatorycomplex is strongly stimulated by oxidative stress. Little is knownabout these oxidative stresses and its contribution to the regulation ofthe alginate biosynthetic pathway, we will conduct a thorough search formutants which no longer respond to stress induced by a redox-cyclingagent that positively affects alginate gene transcription. Followingcharacterization of the mutants obtained, we will clone a regulatoryredox rdx gene which affects the transcription of algD, a gene encodinga key enzyme in the alginate biosynthetic pathway. Characterization ofthe rdx gene will include physical mapping, DNA sequence analysis, andregulation using transcription fusions. Defined mutants defective in theredox stress regulator will be constructed by gene replacement and willbe characterized. To investigate the role of SOD and its regulation inthe global response to oxidant stress in P aeruginosa, we will constructsodB mutants by gene replacement to determine the role of Fe-SOD inoxidant stress and alginate production. We will also characterizethesodA gene of P aeruginosa and generate an sodAsodB double mutant todetermine its sensitivity to oxidative stress and effect on alginateproduction. To determine the role of catalase, and its oxidant-mediatedregulation in P aeruginosa, we will also clone a catalase kat gene byusing genetic complementation strategies. As above, kat mutants and kat- transcriptional fusions will be constructed to evaluate the role ofcatalase in response to various conditions of oxidative stress.

Research Support

Grant: #000238 Investigators:Hassett, Daniel 07-01-2006 -01-12-2012 Cure Finders Use of Acidified Nitrite to Kill Mucoid P. Aeruginosa and S. Aureu Role:PI $217,000.00 Closed Level:Private Non-Profit

Grant: #R01 GM69845 Investigators:Hassett, Daniel 08-01-2004 -07-31-2009 National Institute of General Medical Sciences Role of OxyR in P. Aeruginosa Biofilm Resistance to H202 Role:PI $1,189,245.00 Closed Level:Federal

Grant: #1-R21-AI-055487-01-A1-S0-E0 Investigators:Hassett, Daniel 04-15-2003 -03-31-2005 National Institute of Allergy and Infectious Diseases Proteogenome of Anaerbic P. Aeruginosa in CF Mucus Role:PI $333,498.00 Closed Level:Federal

Investigators:Hassett, Daniel 04-01-2003 -09-30-2004 Cystic Fibrosis Foundation Proteomgenome of Anaerobic P. aeuroginosa in CF Mucus Role:PI $75,000.00 Closed Level:Private Non-Profit

Investigators:Hassett, Daniel 01-01-2005 -10-31-2006 Cystic Fibrosis Foundation Rh1R and NorCB in Anaerobic P. Aeruginosa Biofilms Role:PI $106,624.00 Closed Level:Private Non-Profit

Grant: #2002-UC-10-01-A0-S0-E0 Investigators:Hassett, Daniel 03-01-2001 -02-28-2002 Cystic Fibrosis Foundation Culling P. Aeruginosa Genes Expressed In Vivo Role:PI $64,800.00 Closed Level:Private Non-Profit

Grant: #1-R03-AI-053079-01-A0-S0-E0 Investigators:Hassett, Daniel 09-15-2002 -09-14-2004 National Institutes of Health B.pseudomallei Biofilm Bioterrorism of Drinking Water Role:PI $76,750.00 Closed Level:Federal

Investigators:Hassett, Daniel 06-01-2002 -05-31-2003 Cystic Fibrosis Foundation Proteogenome of Anaerobic P. Aeruginosa in CF Mucus Role:PI $107,487.00 Closed Level:Private Non-Profit

Grant: #5-R01-AI-40541-05-A0-S0-E0 Investigators:Hassett, Daniel 04-01-1998 -03-31-2004 National Institute of Allergy and Infectious Diseases Iron, Fur and Algimate Production in P. Aeruginosa Role:PI $1,027,957.00 Closed Level:Federal

Grant: #CFF R457-02-A0-S0-E0 Investigators:Hassett, Daniel 09-01-1998 -08-31-2000 Cystic Fibrosis Foundation P. Aeruginosa Autoinducers as Biocontrol Agents Role:PI $80,000.00 Closed Level:Private Non-Profit

Grant: #U01 CA84291 Investigators:Boivin, Gregory; Hassett, Daniel; Lowy, Andrew 04-01-2006 -03-31-2008 National Cancer Institute Mouse Models of Gastrointestinal Cancer Role:Collaborator $237,112.00 Active Level:Federal

Grant: #CHMC 103071 Investigators:Hassett, Daniel 09-01-2006 -11-30-2007 Cystic Fibrosis Foundation Nitrite Sensitivity Mechanism in Mucoid P. Aeruginosa Role:PI $40,000.00 Closed Level:Private Non-Profit

Grant: #SRS 004953 Investigators:Hassett, Daniel 03-01-2008 -02-28-2009 Aires Pharmaceuticals, Inc Use of Sodium Nitrite to Kill Biofilms of Cystic Fibrosis-Related Pathogens Role:PI $103,500.00 Closed Level:Industry

Grant: #SRS 005134 Investigators:Hassett, Daniel 04-01-2007 -03-31-2009 Cystic Fibrosis Foundation Role of Antibiotics in Sensitivity of Mucoid P. Aeruginosa to Nitrite Role:PI $90,000.00 Closed Level:Private Non-Profit

Grant: #IIRA-GA9001TG Investigators:Caldwell, Charles; Hassett, Daniel; Solomkin, Joseph 08-08-2008 -06-30-2010 Pfizer, Inc. Induction of PVL Production in Neutrophil Phagosomes: Cell Protection By Bacterial Protein Synthesis Inhibition and Its Effect on Infectious Mortality Role:Collaborator $60,787.50 Active Level:Industry

Grant: #CHMC 104163 Investigators:Britigan, Bradley; Hassett, Daniel; Knittel, James; Reszka, Krzysztof 07-01-2008 -06-30-2010 Cystic Fibrosis Foundation Peroxidases Inactivate Pseudomonas Virulence Factors Role:Collaborator $80,000.00 Closed Level:Private Non-Profit

Grant: #S-845-010-002 / FA8650-08-C-6832 Investigators:Dean, Gary; Hassett, Daniel; Kotb, Malak; Lamkin, Thomas; Nelson, Sandra; Papoian, Ruben; Seibel, William; Strasser, Jane; Wortman, Matthew 08-26-2009 -02-11-2011 Defense Threat Reduction Agency INSIGHTS INteractome Science in Genomic High Throughput Studies Role:Collaborator $3,840,985.00 Active Level:Federal

Grant: #SRS 006574 Investigators:Hassett, Daniel 11-01-2009 -11-30-2012 Cystic Fibrosis Research, Inc. Molecular Basis Underlying Killing of Mucoid Pseudomonas aeruginosa by Nitrite Role:PI $183,500.00 Active Level:Private Non-Profit

Grant: #51447 / R01 AU1075257 Investigators:Hassett, Daniel 09-01-2009 -10-31-2011 National Institute of Allergy and Infectious Diseases Role of BdIA in Biofilm Dispersion and Virulence Properties of P. aeruginosa Role:PI $185,619.00 Closed Level:Federal

Grant: #SRS 006638 Investigators:Hassett, Daniel 12-01-2009 -11-30-2010 Pilus Energy, LLC Developmental Support for Pilus Fuel Cell Reactor (MFRC) Role:PI $15,000.00 Closed Level:Industry

Grant: #39778-5-30624 / U54 AI57153 Investigators:Hassett, Daniel; Kotb, Malak 03-01-2011 -02-28-2013 National Institute of Allergy and Infectious Diseases Systems Approach to Understanding Francisella Host-Pathogen Interactions Role:Collaborator $384,650.00 Closed Level:Federal

Grant: #1 R01 GM094363-01A1 Investigators:Hassett, Daniel; Herr, Andrew 09-01-2011 -06-30-2015 National Institute of General Medical Sciences Studies of Metal-Dependent Intercellular Adhesion in Staphylococcal Biofilms Role:Collaborator $867,394.00 Active Level:Federal

Grant: #VA IPA/ Panmanee Investigators:Hassett, Daniel; Panmanee, Warunya 11-01-2011 -10-31-2013 Department of Veterans Affairs Intergovernmental Personnel Act Agreement for Warunya Panmanee Role:PI $100,535.00 Active Level:Federal

Grant: #VA IPA Award- Daniel Hassett Investigators:Hassett, Daniel; Na, Renuka 03-01-2012 -02-28-2013 Department of Veterans Affairs Interpersonnel Personnel Act Agreement - Renuka Na Role:PI $64,785.00 Active Level:Federal

Grant: #FY12.605.001 \ 1R21AI094487-01A1 Investigators:Hassett, Daniel 04-01-2012 -03-31-2014 National Institute of Allergy and Infectious Diseases Control of Pseudomonas Aeruginosa Quorum Sensing and Alginate Role:PI $21,975.00 Active Level:Federal

Grant: #\ 5R01AI091594-02 Investigators:Hassett, Daniel 05-01-2012 -04-30-2014 National Institute of Allergy and Infectious Diseases The Electricidal Effect, a Novel Anti-Biofilm Strategy Role:PI $141,933.00 Active Level:Federal

Grant: #923-100-005 \ FA8650-10-C-6152 Investigators:Hassett, Daniel 08-01-2012 -12-31-2012 Air Force Office of Scientific Research Technical Goals For 2012 AFRL INSIGHTS Role:PI $41,762.00 Active Level:Federal

Grant: #MOU Investigators:Hassett, Daniel 10-01-2012 -09-30-2015 Daniel Tyler Health and Education Foundation, The The Mechanistic Role of Genes Encoding PmpR, An ABC Transporter and the Lon Protease in Acidified Nitrite (A-NO2-) and Antibiotic Sensitivity in the Important Cystic Fibrosis Pathogen, Role:PI $150,000.00 Active Level:Private Non-Profit

Grant: #ECA Investigators:Browne, Tristan; Hassett, Daniel 10-05-2012 -06-30-2014 Department of Veterans Affairs ECA Role:PI $52,534.00 Active Level:Federal

Grant: #Arch check# 0062 Investigators:Hassett, Daniel 06-01-2014 -05-31-2015 Arch Biopartners Evaluation of a pegylated peptide on biofilm inhibition of pathogenic bacteria on hospital- related materials Role:PI $20,000.00 Awarded Level:Foreign Industry

Grant: #1 R01 GM094363-01A1 Investigators:Hassett, Daniel; Herr, Andrew 09-01-2011 -06-30-2015 National Institute of General Medical Sciences Studies of Metal-Dependent Intercellular Adhesion in Staphylococcal Biofilms Role:Collaborator $1,160,325.00 Active Level:Federal

Grant: #CHMC / R01GM094363 Investigators:Hassett, Daniel 08-01-2014 -07-31-2015 National Institute of General Medical Sciences Studies of metal-dependent intercellular adhesion in Staphylococcal biofilms Role:PI $68,662.00 Awarded Level:Federal

Grant: #R03 AI117587 Investigators:Hassett, Daniel 06-15-2015 -05-31-2017 National Institute of Allergy and Infectious Diseases An innovative treatment for Pneumocystis pneumonia Role:PI $79,021.00 Awarded Level:Federal

Grant: #CBET-1605482 Investigators:Hassett, Daniel 08-01-2016 -07-31-2019 National Science Foundation Microbial Fuel Cell Optimization through Digital Microfluidic Electrochemistry in Single-Bacterial Drops Role:PI $152,000.00 Active Level:Federal

Grant: #ASKEW16P0 Investigators:Askew, David; Hassett, Daniel 09-01-2016 -08-31-2017 Cystic Fibrosis Foundation Neutrophils and ER stress in host adaptation by Aspergillus fumigatus Role:Collaborator $125,000.00 Active Level:Private Non-Profit

Grant: #AHA 18IPA34170157 Investigators:Hassett, Daniel; Kotagiri, Nalinikanth; O'Donnell, Robert 07-01-2018 -06-30-2020 American Heart Association - National Chapter Accurate and early noninvasive imaging of bacterial endocarditis Role:Collaborator $100,000.00 Active Level:Private Non-Profit

Grant: #W81XWH1910125 Investigators:Hassett, Daniel; Kotagiri, Nalinikanth 05-01-2019 -10-31-2020 Department of Defense Site-specific detection of latent tuberculosis through nuclear imaging Role:Collaborator $267,593.00 Awarded Level:Federal

Grant: #R21GM137321 Investigators:Hassett, Daniel; Kotagiri, Nalinikanth 07-01-2020 -06-30-2022 National Institute of General Medical Sciences A Synthetic Approach for Bacterial-Mammalian Cell Binding Role:Collaborator $245,041.00 Awarded Level:Federal

Grant: #ME200246 Investigators:Abdelmalek, Zalfa; Hassett, Daniel; Kotagiri, Nalinikanth 06-01-2021 -05-31-2024 Department of the Army Medical Research Acquisition Activity Engineering Skin Microbiome to Generate Natural Sunscreens for Prevention of Melanoma Role:Collaborator 0.00 Hold Level:Federal

Publications

Peer Reviewed Publications

Panmanee, Warunya; Taylor, Deborah; Shea, Chloe J A; Tang, Hong; Nelson, Sandra; Seibel, William; Papoian, Ruben; Kramer, Ryan; Hassett, Daniel J; Lamkin, Thomas J (2013. ) High-Throughput Screening for Small-Molecule Inhibitors of Staphylococcus epidermidis RP62a Biofilms.Journal of biomolecular screening, , More Information

Major, Tiffany A; Panmanee, Warunya; Mortensen, Joel E; Gray, Larry D; Hoglen, Niel; Hassett, Daniel J (2010. ) Sodium nitrite-mediated killing of the major cystic fibrosis pathogens Pseudomonas aeruginosa, Staphylococcus aureus, and Burkholderia cepacia under anaerobic planktonic and biofilm conditions.Antimicrobial agents and chemotherapy, , 54 (11 ) ,4671-7 More Information

Lau, Gee W; Britigan, Bradley E; Hassett, Daniel J (2005. ) Pseudomonas aeruginosa OxyR is required for full virulence in rodent and insect models of infection and for resistance to human neutrophils.Infection and immunity, , 73 (4 ) ,2550-3 More Information

Hassett, Daniel J; Limbach, Patrick A; Hennigan, Robert F; Klose, Karl E; Hancock, Robert E W; Platt, Mark D; Hunt, Donald F (2003. ) Bacterial biofilms of importance to medicine and bioterrorism: proteomic techniques to identify novel vaccine components and drug targets.Expert opinion on biological therapy, , 3 (8 ) ,1201-7 More Information

Hassett, D J; Elkins, J G; Ma, J F; McDermott, T R (1999. ) Pseudomonas aeruginosa biofilm sensitivity to biocides: use of hydrogen peroxide as model antimicrobial agent for examining resistance mechanisms. Methods in enzymology, , 310 ,599-608

Hassett, D J; Schweizer, H P; Ohman, D E (1995. ) Pseudomonas aeruginosa sodA and sodB mutants defective in manganese- and iron-cofactored superoxide dismutase activity demonstrate the importance of the iron-cofactored form in aerobic metabolism. Journal of bacteriology, , 177 (22 ) ,6330-7

Friedland, L R; Menon, A G; Reising, S F; Ruddy, R M; Hassett, D J (1994. ) Development of a polymerase chain reaction assay to detect the presence of Streptococcus pneumoniae DNA. Diagnostic microbiology and infectious disease, , 20 (4 ) ,187-93

Choi, G C; McQuinn, J S; Jennings, B L; Hassett, D J; Michaels, S E (1994. ) Effect of population size on humidity and ammonia levels in individually ventilated microisolation rodent caging. Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science, , 33 (6 ) ,77-81

Xue, Zheng; Hessler, Christopher M; Panmanee, Warunya; Hassett, Daniel J; Seo, Youngwoo (2013. ) Pseudomonas aeruginosa inactivation mechanism is affected by capsular extracellular polymeric substances reactivity with chlorine and monochloramine.FEMS microbiology ecology, , 83 (1 ) ,101-11 More Information

Deng, Jingyuan; Su, Shengchang; Lin, Xiaodong; Hassett, Daniel J; Lu, Long Jason (2013. ) A statistical framework for improving genomic annotations of prokaryotic essential genes.PloS one, , 8 (3 ) ,e58178 More Information

Su, Shengchang; Hassett, Daniel J (2012. ) Anaerobic Pseudomonas aeruginosa and other obligately anaerobic bacterial biofilms growing in the thick airway mucus of chronically infected cystic fibrosis patients: an emerging paradigm or "Old Hat"?.Expert opinion on therapeutic targets, , 16 (9 ) ,859-73 More Information

Reszka, Krzysztof J; Xiong, Ye; Sallans, Larry; Pasula, Rajamouli; Olakanmi, Oyebode; Hassett, Daniel J; Britigan, Bradley E (2012. ) Inactivation of the potent Pseudomonas aeruginosa cytotoxin pyocyanin by airway peroxidases and nitrite.American journal of physiology. Lung cellular and molecular physiology, , 302 (10 ) ,L1044-56 More Information

Zhang, Minlu; Su, Shengchang; Bhatnagar, Raj K; Hassett, Daniel J; Lu, Long J (2012. ) Prediction and analysis of the protein interactome in Pseudomonas aeruginosa to enable network-based drug target selection.PloS one, , 7 (7 ) ,e41202 More Information

Sanders, Laurie H; Devadoss, Babho; Raja, Geraldine V; O'Connor, Jaime; Su, Shengchang; Wozniak, Daniel J; Hassett, Daniel J; Berdis, Anthony J; Sutton, Mark D (2011. ) Epistatic roles for Pseudomonas aeruginosa MutS and DinB (DNA Pol IV) in coping with reactive oxygen species-induced DNA damage.PloS one, , 6 (4 ) ,e18824 More Information

Kuang, Zhizhou; Hao, Yonghua; Hwang, Sunghei; Zhang, Shiping; Kim, Eunice; Akinbi, Henry T; Schurr, Michael J; Irvin, Randall T; Hassett, Daniel J; Lau, Gee W (2011. ) The Pseudomonas aeruginosa flagellum confers resistance to pulmonary surfactant protein-A by impacting the production of exoproteases through quorum-sensing.Molecular microbiology, , 79 (5 ) ,1220-35 More Information

Deng, Jingyuan; Deng, Lei; Su, Shengchang; Zhang, Minlu; Lin, Xiaodong; Wei, Lan; Minai, Ali A; Hassett, Daniel J; Lu, Long J (2011. ) Investigating the predictability of essential genes across distantly related organisms using an integrative approach.Nucleic acids research, , 39 (3 ) ,795-807 More Information

Collins, Tracy L; Markus, Elizabeth A; Hassett, Daniel J; Robinson, Jayne B (2010. ) The effect of a cationic porphyrin on Pseudomonas aeruginosa biofilms.Current microbiology, , 61 (5 ) ,411-6 More Information

Fung, Carina; Naughton, Sharna; Turnbull, Lynne; Tingpej, Pholawat; Rose, Barbara; Arthur, Jonathan; Hu, Honghua; Harmer, Christopher; Harbour, Colin; Hassett, Daniel J; Whitchurch, Cynthia B; Manos, Jim (2010. ) Gene expression of Pseudomonas aeruginosa in a mucin-containing synthetic growth medium mimicking cystic fibrosis lung sputum.Journal of medical microbiology, , 59 (Pt 9 ) ,1089-100 More Information

Heebink, Loreal V; Pflughoeft-Hassett, Debra F; Hassett, David J (2010. ) Effects of mercury emission control technologies using halogens on coal combustion product chemical properties.Journal of environmental monitoring : JEM, , 12 (3 ) ,608-13 More Information

Hassett, Daniel J; Korfhagen, Thomas R; Irvin, Randall T; Schurr, Michael J; Sauer, Karin; Lau, Gee W; Sutton, Mark D; Yu, Hongwei; Hoiby, Niels (2010. ) Pseudomonas aeruginosa biofilm infections in cystic fibrosis: insights into pathogenic processes and treatment strategies.Expert opinion on therapeutic targets, , 14 (2 ) ,117-30 More Information

Hoffman, Lucas R; Richardson, Anthony R; Houston, Laura S; Kulasekara, Hemantha D; Martens-Habbena, Willm; Klausen, Mikkel; Burns, Jane L; Stahl, David A; Hassett, Daniel J; Fang, Ferric C; Miller, Samuel I (2010. ) Nutrient availability as a mechanism for selection of antibiotic tolerant Pseudomonas aeruginosa within the CF airway.PLoS pathogens, , 6 (1 ) ,e1000712 More Information

Olakanmi, Oyebode; Gunn, John S; Su, Shengchang; Soni, Shilpa; Hassett, Daniel J; Britigan, Bradley E (2010. ) Gallium disrupts iron uptake by intracellular and extracellular Francisella strains and exhibits therapeutic efficacy in a murine pulmonary infection model.Antimicrobial agents and chemotherapy, , 54 (1 ) ,244-53 More Information

Heo, Yun-Jeong; Chung, In-Young; Cho, Wan-Je; Lee, Bo-Young; Kim, Jung-Hoon; Choi, Kyoung-Hee; Lee, Jin-Won; Hassett, Daniel J; Cho, You-Hee (2010. ) The major catalase gene (katA) of Pseudomonas aeruginosa PA14 is under both positive and negative control of the global transactivator OxyR in response to hydrogen peroxide.Journal of bacteriology, , 192 (2 ) ,381-90 More Information

Caldwell, Charles C; Chen, Yi; Goetzmann, Holly S; Hao, Yonghua; Borchers, Michael T; Hassett, Daniel J; Young, Lisa R; Mavrodi, Dmitri; Thomashow, Linda; Lau, Gee W (2009. ) Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis.The American journal of pathology, , 175 (6 ) ,2473-88 More Information

Barraud, Nicolas; Schleheck, David; Klebensberger, Janosch; Webb, Jeremy S; Hassett, Daniel J; Rice, Scott A; Kjelleberg, Staffan (2009. ) Nitric oxide signaling in Pseudomonas aeruginosa biofilms mediates phosphodiesterase activity, decreased cyclic di-GMP levels, and enhanced dispersal.Journal of bacteriology, , 191 (23 ) ,7333-42 More Information

Panmanee, Warunya; Hassett, Daniel J (2009. ) Differential roles of OxyR-controlled antioxidant enzymes alkyl hydroperoxide reductase (AhpCF) and catalase (KatB) in the protection of Pseudomonas aeruginosa against hydrogen peroxide in biofilm vs. planktonic culture.FEMS microbiology letters, , 295 (2 ) ,238-44 More Information

Hassett, Daniel J; Sutton, Mark D; Schurr, Michael J; Herr, Andrew B; Caldwell, Charles C; Matu, Joseph O (2009. ) Pseudomonas aeruginosa hypoxic or anaerobic biofilm infections within cystic fibrosis airways.Trends in microbiology, , 17 (3 ) ,130-8 More Information

Conrady, Deborah G; Brescia, Cristin C; Horii, Katsunori; Weiss, Alison A; Hassett, Daniel J; Herr, Andrew B (2008. ) A zinc-dependent adhesion module is responsible for intercellular adhesion in staphylococcal biofilms.Proceedings of the National Academy of Sciences of the United States of America, , 105 (49 ) ,19456-61 More Information

Wesselkamper, Scott C; Eppert, Bryan L; Motz, Gregory T; Lau, Gee W; Hassett, Daniel J; Borchers, Michael T (2008. ) NKG2D is critical for NK cell activation in host defense against Pseudomonas aeruginosa respiratory infection. Journal of immunology (Baltimore, Md. : 1950), , 181 (8 ) ,5481-9

Havasi, Viktória; Hurst, Casey O; Briles, Travis C; Yang, Fan; Bains, David G; Hassett, Daniel J; Sorscher, Eric (2008. ) Inhibitory effects of hypertonic saline on P. aeruginosa motility.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society, , 7 (4 ) ,267-9 More Information

Panmanee, Warunya; Gomez, Francisco; Witte, David; Pancholi, Vijay; Britigan, Bradley E; Hassett, Daniel J (2008. ) The peptidoglycan-associated lipoprotein OprL helps protect a Pseudomonas aeruginosa mutant devoid of the transactivator OxyR from hydrogen peroxide-mediated killing during planktonic and biofilm culture.Journal of bacteriology, , 190 (10 ) ,3658-69 More Information

Platt, Mark D; Schurr, Michael J; Sauer, Karin; Vazquez, Gustavo; Kukavica-Ibrulj, Irena; Potvin, Eric; Levesque, Roger C; Fedynak, Amber; Brinkman, Fiona S L; Schurr, Jill; Hwang, Sung-Hei; Lau, Gee W; Limbach, Patrick A; Rowe, John J; Lieberman, Michael A; Barraud, Nicolas; Webb, Jeremy; Kjelleberg, Staffan; Hunt, Donald F; Hassett, Daniel J (2008. ) Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions.Journal of bacteriology, , 190 (8 ) ,2739-58 More Information

Hassett, Daniel J; Imlay, James A (2007. ) Bactericidal antibiotics and oxidative stress: a radical proposal.ACS chemical biology, , 2 (11 ) ,708-10 More Information

Morici, Lisa A; Carterson, Alexander J; Wagner, Victoria E; Frisk, Anders; Schurr, Jill R; Höner zu Bentrup, Kerstin; Hassett, Daniel J; Iglewski, Barbara H; Sauer, Karin; Schurr, Michael J (2007. ) Pseudomonas aeruginosa AlgR represses the Rhl quorum-sensing system in a biofilm-specific manner.Journal of bacteriology, , 189 (21 ) ,7752-64 More Information

Melstrom, Kurt A; Kozlowski, Ryan; Hassett, Daniel J; Suzuki, Hideki; Bates, Donna M; Gamelli, Richard L; Shankar, Ravi (2007. ) Cytotoxicity of Pseudomonas secreted exotoxins requires OxyR expression.The Journal of surgical research, , 143 (1 ) ,50-7 More Information

Yoon, Sang Sun; Karabulut, Ahmet C; Lipscomb, John D; Hennigan, Robert F; Lymar, Sergei V; Groce, Stephanie L; Herr, Andrew B; Howell, Michael L; Kiley, Patricia J; Schurr, Michael J; Gaston, Benjamin; Choi, Kyoung-Hee; Schweizer, Herbert P; Hassett, Daniel J (2007. ) Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration.The EMBO journal, , 26 (15 ) ,3662-72 More Information

Barraud, Nicolas; Hassett, Daniel J; Hwang, Sung-Hei; Rice, Scott A; Kjelleberg, Staffan; Webb, Jeremy S (2006. ) Involvement of nitric oxide in biofilm dispersal of Pseudomonas aeruginosa.Journal of bacteriology, , 188 (21 ) ,7344-53 More Information

Morgan, Ryan; Kohn, Steven; Hwang, Sung-Hei; Hassett, Daniel J; Sauer, Karin (2006. ) BdlA, a chemotaxis regulator essential for biofilm dispersion in Pseudomonas aeruginosa.Journal of bacteriology, , 188 (21 ) ,7335-43 More Information

Kong, Fansheng; Young, Lisa; Chen, Yi; Ran, Huimin; Meyers, Melanie; Joseph, Patricia; Cho, You-Hee; Hassett, Daniel J; Lau, Gee W (2006. ) Pseudomonas aeruginosa pyocyanin inactivates lung epithelial vacuolar ATPase-dependent cystic fibrosis transmembrane conductance regulator expression and localization.Cellular microbiology, , 8 (7 ) ,1121-33 More Information

Kashyap, Des R; Botero, Lina M; Lehr, Corinne; Hassett, Daniel J; McDermott, Timothy R (2006. ) A Na+:H+ antiporter and a molybdate transporter are essential for arsenite oxidation in Agrobacterium tumefaciens.Journal of bacteriology, , 188 (4 ) ,1577-84 More Information

Kashyap, Des R; Botero, Lina M; Franck, William L; Hassett, Daniel J; McDermott, Timothy R (2006. ) Complex regulation of arsenite oxidation in Agrobacterium tumefaciens.Journal of bacteriology, , 188 (3 ) ,1081-8 More Information

Yoon, Sang Sun; Coakley, Ray; Lau, Gee W; Lymar, Sergei V; Gaston, Benjamin; Karabulut, Ahmet C; Hennigan, Robert F; Hwang, Sung-Hei; Buettner, Garry; Schurr, Michael J; Mortensen, Joel E; Burns, Jane L; Speert, David; Boucher, Richard C; Hassett, Daniel J (2006. ) Anaerobic killing of mucoid Pseudomonas aeruginosa by acidified nitrite derivatives under cystic fibrosis airway conditions.The Journal of clinical investigation, , 116 (2 ) ,436-46 More Information

Giltner, Carmen L; van Schaik, Erin J; Audette, Gerald F; Kao, Dan; Hodges, Robert S; Hassett, Daniel J; Irvin, Randall T (2006. ) The Pseudomonas aeruginosa type IV pilin receptor binding domain functions as an adhesin for both biotic and abiotic surfaces.Molecular microbiology, , 59 (4 ) ,1083-96 More Information

Noriega, Chris; Hassett, Daniel J; Rowe, John J (2005. ) The mobA gene is required for assimilatory and respiratory nitrate reduction but not xanthine dehydrogenase activity in Pseudomonas aeruginosa.Current microbiology, , 51 (6 ) ,419-24 More Information

Leid, Jeff G; Willson, Carey J; Shirtliff, Mark E; Hassett, Daniel J; Parsek, Matthew R; Jeffers, Alyssa K (2005. ) The exopolysaccharide alginate protects Pseudomonas aeruginosa biofilm bacteria from IFN-gamma-mediated macrophage killing. Journal of immunology (Baltimore, Md. : 1950), , 175 (11 ) ,7512-8

Lau, Gee W; Hassett, Daniel J; Britigan, Bradley E (2005. ) Modulation of lung epithelial functions by Pseudomonas aeruginosa.Trends in microbiology, , 13 (8 ) ,389-97 More Information

Parvatiyar, Kislay; Alsabbagh, Eyad M; Ochsner, Urs A; Stegemeyer, Michelle A; Smulian, Alan G; Hwang, Sung Hei; Jackson, Colin R; McDermott, Timothy R; Hassett, Daniel J (2005. ) Global analysis of cellular factors and responses involved in Pseudomonas aeruginosa resistance to arsenite.Journal of bacteriology, , 187 (14 ) ,4853-64 More Information

Botero, Lina M; D'Imperio, Seth; Burr, Mark; McDermott, Timothy R; Young, Mark; Hassett, Daniel J (2005. ) Poly(A) polymerase modification and reverse transcriptase PCR amplification of environmental RNA.Applied and environmental microbiology, , 71 (3 ) ,1267-75 More Information

Lau, Gee W; Hassett, Daniel J; Ran, Huimin; Kong, Fansheng (2004. ) The role of pyocyanin in Pseudomonas aeruginosa infection.Trends in molecular medicine, , 10 (12 ) ,599-606 More Information

Carterson, Alexander J; Morici, Lisa A; Jackson, Debra W; Frisk, Anders; Lizewski, Stephen E; Jupiter, Ryan; Simpson, Kendra; Kunz, Daniel A; Davis, Scott H; Schurr, Jill R; Hassett, Daniel J; Schurr, Michael J (2004. ) The transcriptional regulator AlgR controls cyanide production in Pseudomonas aeruginosa.Journal of bacteriology, , 186 (20 ) ,6837-44 More Information

Frisk, Anders; Schurr, Jill R; Wang, Guoshun; Bertucci, Donna C; Marrero, Luis; Hwang, Sung Hei; Hassett, Daniel J; Schurr, Michael J (2004. ) Transcriptome analysis of Pseudomonas aeruginosa after interaction with human airway epithelial cells.Infection and immunity, , 72 (9 ) ,5433-8 More Information

Yoon, Sang Sun; Hassett, Daniel J (2004. ) Chronic Pseudomonas aeruginosa infection in cystic fibrosis airway disease: metabolic changes that unravel novel drug targets. Expert review of anti-infective therapy, , 2 (4 ) ,611-23

Lau, Gee W; Ran, Huimin; Kong, Fansheng; Hassett, Daniel J; Mavrodi, Dimitri (2004. ) Pseudomonas aeruginosa pyocyanin is critical for lung infection in mice.Infection and immunity, , 72 (7 ) ,4275-8 More Information

Lauriano, Crystal M; Barker, Jeffrey R; Yoon, Sang-Sun; Nano, Francis E; Arulanandam, Bernard P; Hassett, Daniel J; Klose, Karl E (2004. ) MglA regulates transcription of virulence factors necessary for Francisella tularensis intraamoebae and intramacrophage survival.Proceedings of the National Academy of Sciences of the United States of America, , 101 (12 ) ,4246-9 More Information

Ran, Huimin; Hassett, Daniel J; Lau, Gee W (2003. ) Human targets of Pseudomonas aeruginosa pyocyanin.Proceedings of the National Academy of Sciences of the United States of America, , 100 (24 ) ,14315-20 More Information

Woo, Alison L; Gildea, Lucy A; Tack, Leslie M; Miller, Marian L; Spicer, Zachary; Millhorn, David E; Finkelman, Fred D; Hassett, Daniel J; Shull, Gary E (2002. ) In vivo evidence for interferon-gamma-mediated homeostatic mechanisms in small intestine of the NHE3 Na+/H+ exchanger knockout model of congenital diarrhea.The Journal of biological chemistry, , 277 (50 ) ,49036-46 More Information

Hassett, Daniel J; Cuppoletti, John; Trapnell, Bruce; Lymar, Sergei V; Rowe, John J; Yoon, Sang Sun; Hilliard, George M; Parvatiyar, Kislay; Kamani, Moneesha C; Wozniak, Daniel J; Hwang, Sung Hei; McDermott, Timothy R; Ochsner, Urs A (2002. ) Anaerobic metabolism and quorum sensing by Pseudomonas aeruginosa biofilms in chronically infected cystic fibrosis airways: rethinking antibiotic treatment strategies and drug targets. Advanced drug delivery reviews, , 54 (11 ) ,1425-43

Wyckoff, Timna J O; Thomas, Brittany; Hassett, Daniel J; Wozniak, Daniel J (2002. ) Static growth of mucoid Pseudomonas aeruginosa selects for non-mucoid variants that have acquired flagellum-dependent motility. Microbiology (Reading, England), , 148 (Pt 11 ) ,3423-30

Yoon, Sang Sun; Hennigan, Robert F; Hilliard, George M; Ochsner, Urs A; Parvatiyar, Kislay; Kamani, Moneesha C; Allen, Holly L; DeKievit, Teresa R; Gardner, Paul R; Schwab, Ute; Rowe, John J; Iglewski, Barbara H; McDermott, Timothy R; Mason, Ronald P; Wozniak, Daniel J; Hancock, Robert E W; Parsek, Matthew R; Noah, Terry L; Boucher, Richard C; Hassett, Daniel J (2002. ) Pseudomonas aeruginosa anaerobic respiration in biofilms: relationships to cystic fibrosis pathogenesis. Developmental cell, , 3 (4 ) ,593-603

Heebink, Loreal V; Hassett, David J (2002. ) Release of mercury vapor from coal combustion ash. Journal of the Air & Waste Management Association (1995), , 52 (8 ) ,927-30

Wu, Chieh-Liang; Domenico, Philip; Hassett, Daniel J; Beveridge, Terry J; Hauser, Alan R; Kazzaz, Jeffrey A (2002. ) Subinhibitory bismuth-thiols reduce virulence of Pseudomonas aeruginosa.American journal of respiratory cell and molecular biology, , 26 (6 ) ,731-8 More Information

Britigan, B E; Miller, R A; Hassett, D J; Pfaller, M A; McCormick, M L; Rasmussen, G T (2001. ) Antioxidant enzyme expression in clinical isolates of Pseudomonas aeruginosa: identification of an atypical form of manganese superoxide dismutase.Infection and immunity, , 69 (12 ) ,7396-401 More Information

Frederick, J R; Elkins, J G; Bollinger, N; Hassett, D J; McDermott, T R (2001. ) Factors affecting catalase expression in Pseudomonas aeruginosa biofilms and planktonic cells.Applied and environmental microbiology, , 67 (3 ) ,1375-9 More Information

Kerschen, E J; Irani, V R; Hassett, D J; Rowe, J J (2001. ) snr-1 gene is required for nitrate reduction in Pseudomonas aeruginosa PAO1.Journal of bacteriology, , 183 (6 ) ,2125-31 More Information

Bollinger, N; Hassett, D J; Iglewski, B H; Costerton, J W; McDermott, T R (2001. ) Gene expression in Pseudomonas aeruginosa: evidence of iron override effects on quorum sensing and biofilm-specific gene regulation.Journal of bacteriology, , 183 (6 ) ,1990-6 More Information

Ochsner, U A; Hassett, D J; Vasil, M L (2001. ) Genetic and physiological characterization of ohr, encoding a protein involved in organic hydroperoxide resistance in Pseudomonas aeruginosa.Journal of bacteriology, , 183 (2 ) ,773-8 More Information

Chroneos, Z C; Wert, S E; Livingston, J L; Hassett, D J; Whitsett, J A (2000. ) Role of cystic fibrosis transmembrane conductance regulator in pulmonary clearance of Pseudomonas aeruginosa in vivo. Journal of immunology (Baltimore, Md. : 1950), , 165 (7 ) ,3941-50

Hassett, D J; Ochsner, U A; Groce, S L; Parvatiyar, K; Ma, J F; Lipscomb, J D (2000. ) Hydrogen peroxide sensitivity of catechol-2,3-dioxygenase: a cautionary note on use of xylE reporter fusions under aerobic conditions. Applied and environmental microbiology, , 66 (9 ) ,4119-23

Hassett, D J; Alsabbagh, E; Parvatiyar, K; Howell, M L; Wilmott, R W; Ochsner, U A (2000. ) A protease-resistant catalase, KatA, released upon cell lysis during stationary phase is essential for aerobic survival of a Pseudomonas aeruginosa oxyR mutant at low cell densities. Journal of bacteriology, , 182 (16 ) ,4557-63

Howell, M L; Alsabbagh, E; Ma, J F; Ochsner, U A; Klotz, M G; Beveridge, T J; Blumenthal, K M; Niederhoffer, E C; Morris, R E; Needham, D; Dean, G E; Wani, M A; Hassett, D J (2000. ) AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa, is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide. Journal of bacteriology, , 182 (16 ) ,4545-56

Ochsner, U A; Vasil, M L; Alsabbagh, E; Parvatiyar, K; Hassett, D J (2000. ) Role of the Pseudomonas aeruginosa oxyR-recG operon in oxidative stress defense and DNA repair: OxyR-dependent regulation of katB-ankB, ahpB, and ahpC-ahpF. Journal of bacteriology, , 182 (16 ) ,4533-44

Stewart, P S; Roe, F; Rayner, J; Elkins, J G; Lewandowski, Z; Ochsner, U A; Hassett, D J (2000. ) Effect of catalase on hydrogen peroxide penetration into Pseudomonas aeruginosa biofilms. Applied and environmental microbiology, , 66 (2 ) ,836-8

Hassett, D J; Ma, J F; Elkins, J G; McDermott, T R; Ochsner, U A; West, S E; Huang, C T; Fredericks, J; Burnett, S; Stewart, P S; McFeters, G; Passador, L; Iglewski, B H (1999. ) Quorum sensing in Pseudomonas aeruginosa controls expression of catalase and superoxide dismutase genes and mediates biofilm susceptibility to hydrogen peroxide. Molecular microbiology, , 34 (5 ) ,1082-93

Elkins, J G; Hassett, D J; Stewart, P S; Schweizer, H P; McDermott, T R (1999. ) Protective role of catalase in Pseudomonas aeruginosa biofilm resistance to hydrogen peroxide. Applied and environmental microbiology, , 65 (10 ) ,4594-600

Suh, S J; Silo-Suh, L; Woods, D E; Hassett, D J; West, S E; Ohman, D E (1999. ) Effect of rpoS mutation on the stress response and expression of virulence factors in Pseudomonas aeruginosa. Journal of bacteriology, , 181 (13 ) ,3890-7

Ma, J F; Ochsner, U A; Klotz, M G; Nanayakkara, V K; Howell, M L; Johnson, Z; Posey, J E; Vasil, M L; Monaco, J J; Hassett, D J (1999. ) Bacterioferritin A modulates catalase A (KatA) activity and resistance to hydrogen peroxide in Pseudomonas aeruginosa. Journal of bacteriology, , 181 (12 ) ,3730-42

Kishioka, C; Okamoto, K; Hassett, D J; de Mello, D; Rubin, B K (1999. ) Pseudomonas aeruginosa alginate is a potent secretagogue in the isolated ferret trachea. Pediatric pulmonology, , 27 (3 ) ,174-9

Ma, J F; Hager, P W; Howell, M L; Phibbs, P V; Hassett, D J (1998. ) Cloning and characterization of the Pseudomonas aeruginosa zwf gene encoding glucose-6-phosphate dehydrogenase, an enzyme important in resistance to methyl viologen (paraquat). Journal of bacteriology, , 180 (7 ) ,1741-9

Ma, S; Selvaraj, U; Ohman, D E; Quarless, R; Hassett, D J; Wozniak, D J (1998. ) Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa. Journal of bacteriology, , 180 (4 ) ,956-68

Ma, J F; Phibbs, P V; Hassett, D J (1997. ) Glucose stimulates alginate production and algD transcription in Pseudomonas aeruginosa. FEMS microbiology letters, , 148 (2 ) ,217-21

Hassett, D J; Howell, M L; Ochsner, U A; Vasil, M L; Johnson, Z; Dean, G E (1997. ) An operon containing fumC and sodA encoding fumarase C and manganese superoxide dismutase is controlled by the ferric uptake regulator in Pseudomonas aeruginosa: fur mutants produce elevated alginate levels. Journal of bacteriology, , 179 (5 ) ,1452-9

Hassett, D J; Howell, M L; Sokol, P A; Vasil, M L; Dean, G E (1997. ) Fumarase C activity is elevated in response to iron deprivation and in mucoid, alginate-producing Pseudomonas aeruginosa: cloning and characterization of fumC and purification of native fumC. Journal of bacteriology, , 179 (5 ) ,1442-51

Stevens, M K; Hassett, D J; Radolf, J D; Hansen, E J (1996. ) Cloning and sequencing of the gene encoding the Cu,Zn-superoxide dismutase of Haemophilus ducreyi. Gene, , 183 (1-2 ) ,35-40

Hassett, D J (1996. ) Anaerobic production of alginate by Pseudomonas aeruginosa: alginate restricts diffusion of oxygen. Journal of bacteriology, , 178 (24 ) ,7322-5

Hassett, D J; Sokol, P A; Howell, M L; Ma, J F; Schweizer, H T; Ochsner, U; Vasil, M L (1996. ) Ferric uptake regulator (Fur) mutants of Pseudomonas aeruginosa demonstrate defective siderophore-mediated iron uptake, altered aerobic growth, and decreased superoxide dismutase and catalase activities. Journal of bacteriology, , 178 (14 ) ,3996-4003

Brown, S M; Howell, M L; Vasil, M L; Anderson, A J; Hassett, D J (1995. ) Cloning and characterization of the katB gene of Pseudomonas aeruginosa encoding a hydrogen peroxide-inducible catalase: purification of KatB, cellular localization, and demonstration that it is essential for optimal resistance to hydrogen peroxide. Journal of bacteriology, , 177 (22 ) ,6536-44

DeVries, C A; Hassett, D J; Flynn, J L; Ohman, D E (1995. ) Genetic linkage in Pseudomonas aeruginosa of algT and nadB: mutation in nadB does not affect NAD biosynthesis or alginate production. Gene, , 156 (1 ) ,63-7

Hassett, D J; Cohen, M S (1995. ) RecA versus catalase: who's on first?.The Journal of clinical investigation, , 95 (3 ) ,924-5 More Information

Alcorn, T M; Zheng, H Y; Gunther, M R; Hassett, D J; Cohen, M S (1994. ) Variation in hydrogen peroxide sensitivity between different strains of Neisseria gonorrhoeae is dependent on factors in addition to catalase activity. Infection and immunity, , 62 (5 ) ,2138-40

Hassett, D J; Woodruff, W A; Wozniak, D J; Vasil, M L; Cohen, M S; Ohman, D E (1993. ) Cloning and characterization of the Pseudomonas aeruginosa sodA and sodB genes encoding manganese- and iron-cofactored superoxide dismutase: demonstration of increased manganese superoxide dismutase activity in alginate-producing bacteria. Journal of bacteriology, , 175 (23 ) ,7658-65

Zheng, H Y; Hassett, D J; Bean, K; Cohen, M S (1992. ) Regulation of catalase in Neisseria gonorrhoeae. Effects of oxidant stress and exposure to human neutrophils.The Journal of clinical investigation, , 90 (3 ) ,1000-6 More Information

Hassett, D J; Charniga, L; Bean, K; Ohman, D E; Cohen, M S (1992. ) Response of Pseudomonas aeruginosa to pyocyanin: mechanisms of resistance, antioxidant defenses, and demonstration of a manganese-cofactored superoxide dismutase. Infection and immunity, , 60 (2 ) ,328-36

Hassett, D J; Charniga, L; Cohen, M S (1990. ) recA and catalase in H2O2-mediated toxicity in Neisseria gonorrhoeae. Journal of bacteriology, , 172 (12 ) ,7293-6

Britigan, B E; Hassett, D J; Rosen, G M; Hamill, D R; Cohen, M S (1989. ) Neutrophil degranulation inhibits potential hydroxyl-radical formation. Relative impact of myeloperoxidase and lactoferrin release on hydroxyl-radical production by iron-supplemented neutrophils assessed by spin-trapping techniques. The Biochemical journal, , 264 (2 ) ,447-55

Hassett, D J; Cohen, M S (1989. ) Bacterial adaptation to oxidative stress: implications for pathogenesis and interaction with phagocytic cells. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, , 3 (14 ) ,2574-82

Fu, H S; Hassett, D J; Cohen, M S (1989. ) Oxidant stress in Neisseria gonorrhoeae: adaptation and effects on L-(+)-lactate dehydrogenase activity. Infection and immunity, , 57 (7 ) ,2173-8

Rosen, G M; Hassett, D J; Yankaskas, J R; Cohen, M S (1989. ) Detection of free radicals as a consequence of dog tracheal epithelial cellular xenobiotic metabolism. Xenobiotica; the fate of foreign compounds in biological systems, , 19 (6 ) ,635-43

Pou, S; Hassett, D J; Britigan, B E; Cohen, M S; Rosen, G M (1989. ) Problems associated with spin trapping oxygen-centered free radicals in biological systems. Analytical biochemistry, , 177 (1 ) ,1-6

Hassett, D J; Bean, K; Biswas, G; Cohen, M S (1989. ) The role of hydroxyl radical in chromosomal and plasmid damage in Neisseria gonorrhoeae in vivo. Free radical research communications, , 7 (2 ) ,83-7

Cohen, M S; Britigan, B E; Hassett, D J; Rosen, G M Phagocytes, O2 reduction, and hydroxyl radical. Reviews of infectious diseases, , 10 (6 ) ,1088-96

Cohen, M S; Britigan, B E; Hassett, D J; Rosen, G M (1988. ) Do humans neutrophils form hydroxyl radical? Evaluation of an unresolved controversy. Free radical biology & medicine, , 5 (2 ) ,81-8

Hassett, D J; Britigan, B E; Svendsen, T; Rosen, G M; Cohen, M S (1987. ) Bacteria form intracellular free radicals in response to paraquat and streptonigrin. Demonstration of the potency of hydroxyl radical. The Journal of biological chemistry, , 262 (28 ) ,13404-8

Cohen, M S; Chai, Y; Britigan, B E; McKenna, W; Adams, J; Svendsen, T; Bean, K; Hassett, D J; Sparling, P F (1987. ) Role of extracellular iron in the action of the quinone antibiotic streptonigrin: mechanisms of killing and resistance of Neisseria gonorrhoeae. Antimicrobial agents and chemotherapy, , 31 (10 ) ,1507-13

Choi, Gihoon; Hassett, Daniel J; Choi, Seokheun (2015. ) A paper-based microbial fuel cell array for rapid and high-throughput screening of electricity-producing bacteria.The Analyst, , 140 (12 ) ,4277-83 More Information

Engevik, Melinda A; Yacyshyn, Mary Beth; Engevik, Kristen A; Wang, Jiang; Darien, Benjamin; Hassett, Daniel J; Yacyshyn, Bruce R; Worrell, Roger T (2015. ) Human Clostridium difficile infection: altered mucus production and composition.American journal of physiology. Gastrointestinal and liver physiology, , 308 (6 ) ,G510-24 More Information

Engevik, Melinda A; Engevik, Kristen A; Yacyshyn, Mary Beth; Wang, Jiang; Hassett, Daniel J; Darien, Benjamin; Yacyshyn, Bruce R; Worrell, Roger T (2015. ) Human Clostridium difficile infection: inhibition of NHE3 and microbiota profile.American journal of physiology. Gastrointestinal and liver physiology, , 308 (6 ) ,G497-509 More Information

Su, Shengchang; Bangar, Hansraj; Saldanha, Roland; Pemberton, Adin; Aronow, Bruce; Dean, Gary E; Lamkin, Thomas J; Hassett, Daniel J (2014. ) Construction and characterization of stable, constitutively expressed, chromosomal green and red fluorescent transcriptional fusions in the select agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei.MicrobiologyOpen, , 3 (5 ) ,610-29 More Information

Li, Yi; Petrova, Olga E; Su, Shengchang; Lau, Gee W; Panmanee, Warunya; Na, Renuka; Hassett, Daniel J; Davies, David G; Sauer, Karin (2014. ) BdlA, DipA and induced dispersion contribute to acute virulence and chronic persistence of Pseudomonas aeruginosa.PLoS pathogens, , 10 (6 ) ,e1004168 More Information

Hassett, Daniel J; Borchers, Michael T; Panos, Ralph J (2014. ) Chronic obstructive pulmonary disease (COPD): evaluation from clinical, immunological and bacterial pathogenesis perspectives.Journal of microbiology (Seoul, Korea), , 52 (3 ) ,211-26 More Information

Su, Shengchang; Panmanee, Warunya; Wilson, Jeffrey J; Mahtani, Harry K; Li, Qian; Vanderwielen, Bradley D; Makris, Thomas M; Rogers, Melanie; McDaniel, Cameron; Lipscomb, John D; Irvin, Randall T; Schurr, Michael J; Lancaster, Jack R; Kovall, Rhett A; Hassett, Daniel J (2014. ) Catalase (KatA) plays a role in protection against anaerobic nitric oxide in Pseudomonas aeruginosa.PloS one, , 9 (3 ) ,e91813 More Information

Engevik, Melinda A; Aihara, Eitaro; Montrose, Marshall H; Shull, Gary E; Hassett, Daniel J; Worrell, Roger T (2013. ) Loss of NHE3 alters gut microbiota composition and influences Bacteroides thetaiotaomicron growth.American journal of physiology. Gastrointestinal and liver physiology, , 305 (10 ) ,G697-711 More Information

Su, Shengchang; Saldanha, Roland; Pemberton, Adin; Bangar, Hansraj; Kawamoto, Steven A; Aronow, Bruce; Hassett, Daniel J; Lamkin, Thomas J (2013. ) Characterization of stable, constitutively expressed, chromosomal green and red fluorescent transcriptional fusions in the select agent bacterium, Francisella tularensis Schu S4 and the surrogate type B live vaccine strain (LVS).Applied microbiology and biotechnology, , 97 (20 ) ,9029-41 More Information

Abu, Eme A; Su, Shengchang; Sallans, Larry; Boissy, Raymond E; Greatens, Amanda; Heineman, William R; Hassett, Daniel J (2013. ) Cyclic voltammetric, fluorescence and biological analysis of purified aeruginosin A, a secreted red pigment of Pseudomonas aeruginosa PAO1.Microbiology (Reading, England), , 159 (Pt 8 ) ,1736-47 More Information

Gao, Yang; Hassett, Daniel J; Choi, Seokheun (2017. ) Rapid Characterization of Bacterial Electrogenicity Using a Single-Sheet Paper-Based Electrofluidic Array.Frontiers in bioengineering and biotechnology, , 5 ,44 More Information

Brinkman, Cassandra L; Schmidt-Malan, Suzannah M; Karau, Melissa J; Greenwood-Quaintance, Kerryl; Hassett, Daniel J; Mandrekar, Jayawant N; Patel, Robin (2016. ) Exposure of Bacterial Biofilms to Electrical Current Leads to Cell Death Mediated in Part by Reactive Oxygen Species.PloS one, , 11 (12 ) ,e0168595 More Information

McDaniel, Cameron; Su, Shengchang; Panmanee, Warunya; Lau, Gee W; Browne, Tristan; Cox, Kevin; Paul, Andrew T; Ko, Seung-Hyun B; Mortensen, Joel E; Lam, Joseph S; Muruve, Daniel A; Hassett, Daniel J (2016. ) A Putative ABC Transporter Permease Is Necessary for Resistance to Acidified Nitrite and EDTA in Pseudomonas aeruginosa under Aerobic and Anaerobic Planktonic and Biofilm Conditions.Frontiers in microbiology, , 7 ,291 More Information

Shreeram, Devesh Dadhich; Hassett, Daniel J; Schaefer, Dale W (2016. ) Urine-powered microbial fuel cell using a hyperpiliated pilT mutant of Pseudomonas aeruginosa.Journal of industrial microbiology & biotechnology, , 43 (1 ) ,103-7 More Information

Panmanee, Warunya; Su, Shengchang; Schurr, Michael J; Lau, Gee W; Zhu, Xiaoting; Ren, Zhaowei; McDaniel, Cameron T; Lu, Long J; Ohman, Dennis E; Muruve, Daniel A; Panos, Ralph J; Yu, Hongwei D; Thompson, Thomas B; Tseng, Boo Shan; Hassett, Daniel J (2019. ) The anti-sigma factor MucA of Pseudomonas aeruginosa: Dramatic differences of a mucA22 vs. a ?mucA mutant in anaerobic acidified nitrite sensitivity of planktonic and biofilm bacteria in vitro and during chronic murine lung infection.PloS one, , 14 (6 ) ,e0216401 More Information

Tahernia, Mehdi; Mohammadifar, Maedeh; Hassett, Daniel J; Choi, Seokheun (2018. ) A Fully-Papertronic Biosensing Array for High-Throughput Characterization of Microbial Electrogenicity.Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, , 2018 ,1-4 More Information

Shreeram, Devesh D; Panmanee, Warunya; McDaniel, Cameron T; Daniel, Susan; Schaefer, Dale W; Hassett, Daniel J (2018. ) Effect of impaired twitching motility and biofilm dispersion on performance of Pseudomonas aeruginosa-powered microbial fuel cells.Journal of industrial microbiology & biotechnology, , 45 (2 ) ,103-109 More Information

Li, Ming; Zhou, Minghua; Tian, Xiaoyu; Tan, Chaolin; McDaniel, Cameron T; Hassett, Daniel J; Gu, Tingyue Microbial fuel cell (MFC) power performance improvement through enhanced microbial electrogenicity.Biotechnology advances, , 36 (4 ) ,1316-1327 More Information

Fenker, Daniel E; McDaniel, Cameron T; Panmanee, Warunya; Panos, Ralph J; Sorscher, Eric J; Sabusap, Carleen; Clancy, John P; Hassett, Daniel J (2018. ) A Comparison between Two Pathophysiologically Different yet Microbiologically Similar Lung Diseases: Cystic Fibrosis and Chronic Obstructive Pulmonary Disease.International journal of respiratory and pulmonary medicine, , 5 (2 ) , More Information

Panmanee, Warunya; Charoenlap, Nisanart; Atichartpongkul, Sopapan; Mahavihakanont, Aekkapol; Whiteside, Matthew D; Winsor, Geoff; Brinkman, Fiona S L; Mongkolsuk, Skorn; Hassett, Daniel J (2017. ) The OxyR-regulated phnW gene encoding 2-aminoethylphosphonate:pyruvate aminotransferase helps protect Pseudomonas aeruginosa from tert-butyl hydroperoxide.PloS one, , 12 (12 ) ,e0189066 More Information

Bogue, Amy L; Panmanee, Warunya; McDaniel, Cameron T; Mortensen, Joel E; Kamau, Edwin; Actis, Luis A; Johannigman, Jay A; Schurr, Michael J; Satish, Latha; Kotagiri, Nalinikanth; Hassett, Daniel J (2021. ) AB569, a non-toxic combination of acidified nitrite and EDTA, is effective at killing the notorious Iraq/Afghanistan combat wound pathogens, multi-drug resistant Acinetobacter baumannii and Acinetobacter spp.PloS one, , 16 (3 ) ,e0247513 More Information

Tahernia, Mehdi; Mohammadifar, Maedeh; Hassett, Daniel J; Choi, Seokheun (2020. ) A portable papertronic sensing system for rapid, high-throughput, and visual screening of bacterial electrogenicity.Biosensors & bioelectronics, , 165 ,112348 More Information

Hassett, Daniel J; Meyer, Thomas J (2020. ) A Novel Bactericidal Drug Effective Against Gram-Positive and Gram-Negative Pathogenic Bacteria: Easy as AB569.DNA and cell biology, , 39 (9 ) ,1473-1477 More Information

Tahernia, Mehdi; Mohammadifar, Maedeh; Gao, Yang; Panmanee, Warunya; Hassett, Daniel J; Choi, Seokheun (2020. ) A 96-well high-throughput, rapid-screening platform of extracellular electron transfer in microbial fuel cells.Biosensors & bioelectronics, , 162 ,112259 More Information

McDaniel, Cameron T; Panmanee, Warunya; Winsor, Geoffrey L; Gill, Erin; Bertelli, Claire; Schurr, Michael J; Dongare, Prateek; Paul, Andrew T; Ko, Seung-Hyun B; Lau, Gee W; Dasgupta, Nupur; Bogue, Amy L; Miller, William E; Mortensen, Joel E; Haslam, David B; Dexheimer, Phillip; Muruve, Daniel A; Aronow, Bruce J; Forbes, Malcolm D E; Danilczuk, Marek; Brinkman, Fiona S L; Hancock, Robert E W; Meyer, Thomas J; Hassett, Daniel J (2020. ) AB569, a nontoxic chemical tandem that kills major human pathogenic bacteria.Proceedings of the National Academy of Sciences of the United States of America, , 117 (9 ) ,4921-4930 More Information

Lafond, Maxime; Shekhar, Himanshu; Panmanee, Warunya; Collins, Sydney D; Palaniappan, Arunkumar; McDaniel, Cameron T; Hassett, Daniel J; Holland, Christy K (2019. ) Bactericidal Activity of Lipid-Shelled Nitric Oxide-Loaded Microbubbles.Frontiers in pharmacology, , 10 ,1540 More Information

Keywords

Alginate,Catalase,Fusion Gene,Gene Mutation,Cystic Fibrosis,Genetic Mapping,Regulatory Gene,Oxidative Stress,Molecular Cloning,Molecular Pathology,Gene Complementation,Genetic Transduction,Superoxide Dismutase,Genetic Transcription,Nucleic Acid Sequence,Pseudomonas Aeruginosa,Opportunistic Infection

Contact Information

Academic - Medical Sciences Building
231 Albert Sabin Way
Cincinnati  45267
Phone: 513-558-1154
daniel.hassett@uc.edu