Danuta H. Malinowska , PhD

Our research interests are in the pumps, carriers, and ion channels of the epithelia. Our research has focused on transport proteins and transport processes of the gastrointestinal tract and lung. In recent years, potassium and chloride channel regulation, pharmacology, and molecular engineering have become increasingly important tools with which to study the functional mechanisms of these proteins. More recently, as the X-ray crystal structures of these proteins have become available, we have combined these types of studies with computational chemistry approaches in attempts to understand the fundamental processes of protein gating that underlie function and gating of these channels. We have also placed these transport proteins into synthetic membranes in order to obtain more detailed information regarding protein function that cannot be obtained from purely biological membranes.

Grant: #AFOSR FA9550-07-1-0257 Investigators:Cuppoletti, John; Malinowska, Danuta 03-15-2007 -11-30-2009 Air Force Office of Scientific Research Mechanisms of Ion Transport and Barrier Function of Membranes at Extremes of Temperature and Ionic Conditions Role:Collaborator $450,000.00 Closed Level:Federal

Published Abstracts

Cuppoletti J, Malinowska DH, Tewari KP, Chakrabarti J & Ueno R (2010. ) Methadone, but not morphine inhibits stimulated chloride currents in ClC-2 transfected cells in culture. .[Abstract]DDW 2010, New Orleans LA, May 2010.,

Cuppoletti J, Malinowska DH, Chakrabarti J, Sekida T & Ueno R (2010. ) Lubiprostone suppresses growth of colon cancer cells in vitro and in vivo. .[Abstract]Am College of Gastroenterology meeting 2010, San Antonio TX, October 2010,

Cuppoletti J, Malinowska DH & Chakrabarti J (2010. ) Lubiprostone, but not linaclotide, protects T84 cells against damage to barrier function by interferon-γ. Am College of Gastroenterology meeting 2010, San Antonio TX, October 2010,

Peer Reviewed Publications

Cuppoletti J, Ferrante CJ & Malinowska DH (2009. ) Engineered Ion Channels on Synthetic Flexible Membranes: Ion Channel Devices With Focus on Peptides. ASME 2009 Conference on Smart Materials, Adaptive Structures and Intelligent Systems (SMASIS2009), September 21–23, 2009, Oxnard, California, USA. Sponsor: Aerospace Division. , , Volume 2: Multifunctional Materials; Enabling Technologies and Integrated System Design; Structural Health Monitoring/NDE; Bio-Inspired Smart Materials and Structures. ISBN: 978-0-7918-4897-5. Section: Bio-Inspired Smart Materials and Structures ,597-601

Cuppoletti, John; Tewari, Kirti P; Chakrabarti, Jayati; Malinowska, Danuta H (2017. ) Identification of the fatty acid activation site on human ClC-2.American journal of physiology. Cell physiology, , 312 (6 ) ,C707-C723 More Information

Nighot, Meghali P; Nighot, Prashant K; Ma, Thomas Y; Malinowska, Danuta H; Shull, Gary E; Cuppoletti, John; Blikslager, Anthony T (2015. ) Genetic Ablation of the ClC-2 Cl- Channel Disrupts Mouse Gastric Parietal Cell Acid Secretion.PloS one, , 10 (9 ) ,e0138174 More Information

Cuppoletti, John; Chakrabarti, Jayati; Tewari, Kirti P; Malinowska, Danuta H (2014. ) Differentiation between human ClC-2 and CFTR Cl- channels with pharmacological agents.American journal of physiology. Cell physiology, , 307 (5 ) ,C479-92 More Information

Cuppoletti, John; Chakrabarti, Jayati; Tewari, Kirti; Malinowska, Danuta H (2013. ) Methadone but not morphine inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents.Cell biochemistry and biophysics, , 66 (1 ) ,53-63 More Information

Cuppoletti, John; Malinowska, Danuta H; Tewari, Kirti P; Chakrabarti, Jayati; Ueno, Ryuji (2012. ) Unoprostone isopropyl and metabolite M1 activate BK channels and prevent ET-1-induced [Ca²?]i increases in human trabecular meshwork and smooth muscle.Investigative ophthalmology & visual science, , 53 (9 ) ,5178-89 More Information

Cuppoletti, John; Blikslager, Anthony T; Chakrabarti, Jayati; Nighot, Prashant K; Malinowska, Danuta H (2012. ) Contrasting effects of linaclotide and lubiprostone on restitution of epithelial cell barrier properties and cellular homeostasis after exposure to cell stressors.BMC pharmacology, , 12 ,3 More Information

Other Publications

Cuppoletti J & Malinowska DH (2010. ) Ion channels of the epithelia of the gastrointestinal tract. Physiology of the Gastrointestinal Tract, ed. Johnson LR .Physiology of the Gastrointestinal Tract, ed. Johnson LR, Elsevier

Cuppoletti J, Malinowska DH, Tewari KP, Abey JE, Venkatraman L. Chakrabarti J & Ueno R (2010. ) Cobiprostone activates native human airway and recombinant ClC-2 Cl channels. Am J Resp Cell Mol Bio .Am J Resp Cell Mol Bio.,

Cuppoletti J, Chakrabarti J & Malinowska DH (2010. ) Lubiprostone, but not linoclatide, protects T84 cells against damage to barrier function by interferon-γ. POLM .POLM,

Cuppoletti J, Chakrabarti J, Tewari KP, Malinowska DH & Ueno R (2010. ) Methadone, but not morphine inhibits stimulated chloride currents in ClC-2 transfected cells in culture. POLM .POLM,