Giovanni Pauletti

Giovanni M. Pauletti

Assoc Professor

Medical Sciences Building

3109 D

Pharmacy Pauletti Lab - 0514

Office: Medical Sciences Building (MSB) 3109D
231 Albert Sabin Way
Cincinnati, Ohio 45267-0514

Professional Summary

Dr. Pauletti is an Associate Professor of Biopharmaceutics & Pharmacokinetics at the James L. Winkle College of Pharmacy. His research interests focus on molecular pharmaceutics with an emphasis on membrane transport. Current activities include rational design, fabrication, and evaluation of innovative, non-viral nanocarriers as vectors for targeted drug delivery applications to improve therapeutic efficacy of small molecules, biotechnology drugs, and antitumor agents. Dr. Pauletti has published more than 100 scientific abstracts and papers. He is an inventor on 15 U.S. patents and patent applications protecting various drug delivery technologies and contributed to one GenBank entry. Dr. Pauletti served on more than 40 MS/PhD committees and is engaged in the education of professional PharmD and MS/PhD students enrolled in Pharmaceutical Sciences and Engineering graduate programs. As an advisor to pharmaceutical companies, Dr. Pauletti has facilitated preclinical and early clinical development of different molecular entities relevant to women's health and cancer therapy.

Education

MPharm - Pharmacy (1987), Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland,

PhD - Biopharmaceutics/Pharmacokinetics (1994), Swiss Federal Institute of Technology Zurich (ETHZ), Switzerland,

Research Support

UC Research Council "Development of Nonviral DNA Delivery Systems for Placental Gene Therapy"

Bill & Melinda Gates Foundation "Tampon-Like Foam Device for Dual-Purpose Contraception"

National Institute of General Medical Sciences "In vitro Human Tissue Model for Intravaginal Drug Delivery"

Grant: #R15 GM067639 Investigators:Pauletti, Giovanni 04-10-2003 -03-31-2007 National Institute of General Medical Sciences Vaginal Drug Delivery of P-Glycoprotein Substrates Role:PI $148,953.00 Closed Level:Federal

Grant: #HD048512 Investigators:Pauletti, Giovanni 03-01-2005 -02-28-2007 National Institute of Child Health and Human Development Peptide Ligands for Placental Transcytosis Receptors Role:PI $410,127.00 Closed Level:Federal

Grant: #PHRMA-02-01-A0-S0-E0 Investigators:Pauletti, Giovanni 01-01-2000 -12-31-2000 Pharmaceutical Research and Manufacturers of America Rational Prodrug Design to Overcome Drug Resistance Role:PI $25,000.00 Closed Level:Industry

Investigators:Pauletti, Giovanni 07-01-2002 -06-30-2004 American Cancer Society - Ohio Chapter Growth Inhibition of Metastatic Pancreatic Cancer in vivo Role:PI $19,991.00 Closed Level:Private Non-Profit

Grant: #DAMD17-00-1-0202-03-A0-S0-E0 Investigators:Pauletti, Giovanni 07-01-2000 -06-30-2003 Department of the Army Improved Delivery of Doxorubicin Into Drug-Resistant Breast Cancer Cells Role:PI $315,198.00 Closed Level:Federal

Grant: #OPP1025252 Investigators:Pauletti, Giovanni 11-01-2010 -04-30-2012 Bill & Melinda Gates Foundation Tampon-like Foam Device for Dual-purpose Contraception Role:PI $100,000.00 Closed Level:Private Non-Profit

Grant: #MAT / 1 R43 GM093563-01 Investigators:Pauletti, Giovanni 09-06-2010 -03-05-2012 National Institute of General Medical Sciences In Vitro Human Tissue Model for Intravaginal Delivery Role:PI $39,250.00 Closed Level:Federal

Grant: #Master Study Agreement Investigators:Pauletti, Giovanni 10-01-2011 -10-02-2016 Dow Corning Corporation In Vitro Permeation Properties of Polymethylsiloxanes Role:PI $141,215.00 Active Level:Industry

Grant: #JS-2658 Fellowship - Allam Investigators:Pauletti, Giovanni 09-24-2012 -09-23-2013 Embassy of the Arab Republic of Egypt Design and Evaluation of Medicated Polymeric Nanoparticles for Tumor Therapy Role:PI $10,000.00 Active Level:Foreign Other

Grant: #JS-2661 Investigators:Pauletti, Giovanni 09-24-2012 -09-23-2013 Embassy of the Arab Republic of Egypt Formulation and Delivery of Gene with Peptide-targeted Nanocarriers Role:PI $10,750.00 Active Level:Foreign Other

Grant: #OPP1087022 Investigators:Pauletti, Giovanni; Steckl, Andrew 05-01-2013 -10-31-2014 Bill & Melinda Gates Foundation Paper Based Diagnostics for Tropical Disease Detection Role:Collaborator $100,000.00 Active Level:Private Non-Profit

Grant: #Noura Hassan Abdellah Ali, JS-2661 Investigators:Pauletti, Giovanni 09-20-2013 -09-19-2014 Embassy of the Arab Republic of Egypt Formulation and Delivery of Gene With Peptide-targeted Nanocarriers Role:PI $10,750.00 Awarded Level:Foreign Other

Grant: #Scholarship-Basma Investigators:Pauletti, Giovanni 12-13-2013 -12-31-2014 Embassy of the Arab Republic of Egypt Solid Lipid Nanoparticle Formulation for Alendronate - Basma Nagy Abd El Hamid Role:PI $10,500.00 Awarded Level:Foreign Other

Grant: #Scholarship-Nermin Investigators:Pauletti, Giovanni 12-13-2013 -12-31-2014 Embassy of the Arab Republic of Egypt Therapeutic Efficacy of Oral Heparin - Scholar Nermin El Sayed El Eraky Ali Role:PI $10,500.00 Awarded Level:Foreign Other

Grant: #URC Interdisciplinary Awards Investigators:Hegener, Michael; Pauletti, Giovanni; Steckl, Andrew 04-01-2014 -03-31-2015 UC's University Research Council Clinical Assessment of a Novel Paper-based Microfluidics Device: Towards Patient-Controlled Monitoring of Blood Coagulation Role:Collaborator $25,000.00 Active Level:Internal UC

Grant: #Scholarship-Ahmed Investigators:Pauletti, Giovanni 01-01-2015 -12-31-2015 Embassy of the Arab Republic of Egypt "Tumor-targeting Chitosan Nanoparticles" - Scholar: Sheryhan Ahmed Role:PI $10,500.00 Awarded Level:Foreign Other

Grant: #NSF IIP-1500236 Investigators:Hegener, Michael; Pauletti, Giovanni; Steckl, Andrew 04-01-2015 -09-30-2016 National Science Foundation Developing an Engineering Prototype for Ultra-Low-Cost Blood Coagulation Diagnostics Using Paper-Based Microfluidics Role:Collaborator $200,000.00 Awarded Level:Federal

Grant: #MatTek sub R44GM108164 Investigators:Pauletti, Giovanni 02-01-2016 -01-31-2017 National Institute of General Medical Sciences Validation of In an Vitro Human Small Intestine Model for Pharmaceutical Development Role:PI $86,982.00 Active Level:Federal

Publications

Published Abstracts

Eleraky, N.E., Swarnakar, N.K., Mohamed, D.F., Attia, M.A., and Pauletti, G.M. (2016. )Permeation-enhancing Nanoparticle Formulation to Enable Oral Absorption of Enoxaparin. 30th Annual Meeting of the AAPS, November 13 – 17, Denver, CO (#25R0900) ,

Abd El-Hamid, B.N., Swarnakar, N.K., Soliman, G.M., Shafie, M.A., and Pauletti, G.M. (2016. )Encapsulation of Alendronate-Polyethyleneimine Complexes into Nanostructured Lipid Carriers Enhances Intestinal Permeation in vitro. 30th Annual Meeting of the AAPS, , November 13 – 17, Denver, CO (#23T1000) ,

Potter, S.J., Domoradzki, J.Y., and Pauletti, G.M. (2015. )Comparative in vitro Absorption Models to Estimate Dietary Assimilation of Pharmaceuticals in Fish. 29th Annual Meeting of the AAPS, , October 25 – 29, Orlando, FL (#T2006) ,

Eleraky, N.E., Mohamed, D.F., Attia, M.A., and Pauletti, G.M. (2015. )Thermodynamic Analysis of the Interaction between Low Molecular Weight Heparin and Cetyltrimethylammonium Bromide. 29th Annual Meeting of the AAPS, , October 25 – 29, Orlando, FL (#T3109) ,

Abd El-Hamid, B.N., Soliman, G.M., Shafie, M.A., and Pauletti, G.M. (2015. )Hydrophobic Ion Pairing to Augment Loading Efficiency of Alendronate in Nanostructured Lipid Carriers. 29th Annual Meeting of the AAPS,, October 25 – 29, Orlando, FL (#W4098) ,

Peer Reviewed Publications

Huth, Ch., Shi, D., Wang, F., Carrahar, D., Lian, J., Lu, F., Zhang, J., Ewing, R.C., and Pauletti, G.M. Phospholipid Assembly on Superparamagnetic Nanoparticles for Thermoresponsive Drug Delivery Applications .NanoLIFE, ,

Wen, H.Y., Dong, H.Q., Xie, W.J., Li, Y.Y., Wang, K., Pauletti, G.M., and Shi, D. (2011. )Rapidly Disassembling Nanomicelles with Disulfide-linked PEG Shell for Glutathione-mediated Intracellular Drug Delivery.ChemComm., ,47 ,3550-3552

Basha, S., Vaidhyanathan, S., and Pauletti, G.M. (2011. )Selection of Peptide Ligands for Human Placental Transcytosis Systems using in vitro Phage Display.Methods Mol. Biol., ,716 ,141-156

Cho, H., Dong, Z, Pauletti, G.M., Zhang, J., Xu, H., Gu, H., Wang, L., Ewing, R.C., Huth, Ch., Wang, F., and Shi, D. (2010. )Fluorescent Superparamagnetic Nanospheres for Drug Storage, Targeting, and Imaging - A Multifunctional Nanocarrier System for Cancer Diagnosis and Treatment.ACS Nano, ,4 ,5389-5404

Karkare, S., Chhipa, R., Liu, X., Henry, H., Gasilina, A., Nassar, N., Pauletti, G.M., Ghosh, P.K., and Dasgupta, B. (2014. )Direct Inhibition of Retinoblastoma Phosphorylation by Nimbolide Causes Cell Cycle Arrest and Suppresses Glioblastoma Growth.Clinical Cancer Res., ,20 (1 ),199-212

Allam, Ayat A; Sadat, Md Ehsan; Potter, Sarah J; Mast, David B; Mohamed, Dina F; Habib, Fawzia S; Pauletti, Giovanni M (2013. )Stability and magnetically induced heating behavior of lipid-coated Fe3O4 nanoparticles.Nanoscale research letters, ,8 (1 ),426

Wang, Feng; Pauletti, Giovanni M; Wang, Juntao; Zhang, Jiaming; Ewing, Rodney C; Wang, Yilong; Shi, Donglu (2013. )Dual surface-functionalized Janus nanocomposites of polystyrene/Fe?O?@SiO? for simultaneous tumor cell targeting and stimulus-induced drug release.Advanced materials (Deerfield Beach, Fla.), ,25 (25 ),3485-9

Cai, Xiaojun; Dong, Chunyan; Dong, Haiqing; Wang, Gangmin; Pauletti, Giovanni M; Pan, Xiaojing; Wen, Huiyun; Mehl, Isaac; Li, Yongyong; Shi, Donglu (2012. )Effective gene delivery using stimulus-responsive catiomer designed with redox-sensitive disulfide and acid-labile imine linkers.Biomacromolecules, ,13 (4 ),1024-34

Li, Xue-Quan; Wen, Hui-Yun; Dong, Hai-Qing; Xue, Wei-Ming; Pauletti, Giovanni M; Cai, Xiao-Jun; Xia, Wen-Juan; Shi, Donglu; Li, Yong-Yong (2011. )Self-assembling nanomicelles of a novel camptothecin prodrug engineered with a redox-responsive release mechanism.Chemical communications (Cambridge, England), ,47 (30 ),8647-9

Wen, Hui-Yun; Dong, Hai-Qing; Xie, Wen-juan; Li, Yong-Yong; Wang, Kang; Pauletti, Giovanni M; Shi, Dong-Lu (2011. )Rapidly disassembling nanomicelles with disulfide-linked PEG shells for glutathione-mediated intracellular drug delivery.Chemical communications (Cambridge, England), ,47 (12 ),3550-2

Basha, Saleem; Vaidhyanathan, Shruthi; Pauletti, Giovanni M (2011. )Selection of peptide ligands for human placental transcytosis systems using in vitro phage display.Methods in molecular biology (Clifton, N.J.), ,716 ,141-56

Cho, Hoon-Sung; Dong, Zhongyun; Pauletti, Giovanni M; Zhang, Jiaming; Xu, Hong; Gu, Hongchen; Wang, Lumin; Ewing, Rodney C; Huth, Christopher; Wang, Feng; Shi, Donglu (2010. )Fluorescent, superparamagnetic nanospheres for drug storage, targeting, and imaging: a multifunctional nanocarrier system for cancer diagnosis and treatment.ACS nano, ,4 (9 ),5398-404

Kawedia, Jitesh D; Jiang, Mengmeng; Kulkarni, Amit; Waechter, Holly E; Matlin, Karl S; Pauletti, Giovanni M; Menon, Anil G (2008. )The protein kinase A pathway contributes to Hg2+-induced alterations in phosphorylation and subcellular distribution of occludin associated with increased tight junction permeability of salivary epithelial cell monolayers.The Journal of pharmacology and experimental therapeutics, ,326 (3 ),829-37

Narang, Vishal S; Pauletti, Giovanni M; Gout, Peter W; Buckley, Donna J; Buckley, Arthur R (2007. )Sulfasalazine-induced reduction of glutathione levels in breast cancer cells: enhancement of growth-inhibitory activity of Doxorubicin.Chemotherapy, ,53 (3 ),210-7

Dixit, Santosh G; Zingarelli, Basilia; Buckley, Donna J; Buckley, Arthur R; Pauletti, Giovanni M (2005. )Nitric oxide mediates increased P-glycoprotein activity in interferon-{gamma}-stimulated human intestinal cells.American journal of physiology. Gastrointestinal and liver physiology, ,288 (3 ),G533-40

Narang, Vishal S; Pauletti, Giovanni M; Gout, Peter W; Buckley, Donna J; Buckley, Arthur R Suppression of cystine uptake by sulfasalazine inhibits proliferation of human mammary carcinoma cells. Anticancer research, ,23 (6C ),4571-9

D'Souza, Vanessa M; Buckley, Donna J; Buckley, Arthur R; Pauletti, Giovanni M (2003. )Extracellular glucose concentration alters functional activity of the intestinal oligopeptide transporter (PepT-1) in Caco-2 cells.Journal of pharmaceutical sciences, ,92 (3 ),594-603

D'Souza, Vanessa M; Shertzer, Howard G; Menon, Anil G; Pauletti, Giovanni M (2003. )High glucose concentration in isotonic media alters caco-2 cell permeability.AAPS pharmSci, ,5 (3 ),E24

Plageman, Lauren R; Pauletti, Giovanni M; Skau, Kenneth A (2002. )Characterization of acetylcholinesterase in Caco-2 cells. Experimental biology and medicine (Maywood, N.J.), ,227 (7 ),480-6

Gangwar, S; Pauletti, G M; Siahaan, T J; Stella, V J; Borchardt, R T (1999. )Synthesis of an esterase-sensitive cyclic prodrug of a model hexapeptide having enhanced membrane permeability and enzymatic stability using an acyloxyalkoxy promoiety.Methods in molecular medicine, ,23 ,37-51

Gudmundsson, O S; Pauletti, G M; Wang, W; Shan, D; Zhang, H; Wang, B; Borchardt, R T (1999. )Coumarinic acid-based cyclic prodrugs of opioid peptides that exhibit metabolic stability to peptidases and excellent cellular permeability. Pharmaceutical research, ,16 (1 ),7-15

Sudoh, M; Pauletti, G M; Yao, W; Moser, W; Yokoyama, A; Pasternak, A; Sprengeler, P A; Smith, A B; Hirschmann, R; Borchardt, R T (1998. )Transport characteristics of peptidomimetics. Effect of the pyrrolinone bioisostere on transport across Caco-2 cell monolayers. Pharmaceutical research, ,15 (5 ),719-25

T Borchardt R, ; Jeffrey, ; Siahaan, ; Gangwar, ; Pauletti, (1997. )Improvement of oral peptide bioavailability: Peptidomimetics and prodrug strategies. Advanced drug delivery reviews, ,27 (2-3 ),235-256

Okumu, F W; Pauletti, G M; Vander Velde, D G; Siahaan, T J; Borchardt, R T (1997. )Effect of restricted conformational flexibility on the permeation of model hexapeptides across Caco-2 cell monolayers. Pharmaceutical research, ,14 (2 ),169-75

Pauletti, G M; Okumu, F W; Borchardt, R T (1997. )Effect of size and charge on the passive diffusion of peptides across Caco-2 cell monolayers via the paracellular pathway. Pharmaceutical research, ,14 (2 ),164-8

Pauletti, G M; Gangwar, S; Wang, B; Borchardt, R T (1997. )Esterase-sensitive cyclic prodrugs of peptides: evaluation of a phenylpropionic acid promoiety in a model hexapeptide. Pharmaceutical research, ,14 (1 ),11-7

Pauletti, G M; Gangwar, S; Okumu, F W; Siahaan, T J; Stella, V J; Borchardt, R T (1996. )Esterase-sensitive cyclic prodrugs of peptides: evaluation of an acyloxyalkoxy promoiety in a model hexapeptide. Pharmaceutical research, ,13 (11 ),1615-23

Dunn, A.W., Zhang, Y., Mast, D.B., Pauletti, G.M., Xu, H., Zhang, J., Ewing, R.C., and Shi, D. (2016. )In-vitro Depth-dependent Hyperthermia of Human Mammary Gland Adenocarcinoma.Mater. Sci. Eng. C, ,69 ,12-16

Tucker, G., DeSilva, B., Dressman, J., Ito, M., Kumamoto, T., Mager, D., Mahler, H.C., Maitland-van der Zee, A.H., Pauletti, G.M., Sasaki, H., Shah, V., Tang, D., and Ward, M. (2016. )Current Challenges and Potential Opportunities for the Pharmaceutical Sciences to Make Global Impact: An FIP Perspective.J. Pharm. Sci., ,105 (9 ),344-351

Abd Ellah, N., Taylor, L., Ayres, N., Elmahdy, M.M., Fetih, G.N., Ibrahim, E.A., and Pauletti, G.M. (2016. )NF-κB Decoy Polyplexes Decrease P-glycoprotein-mediated Multidrug Resistance in Colorectal Cancer Cells.Cancer Gene Ther., ,23 (5 ),149-155

Dunn, A.W., Sadat, M.E., Mast, D.B., Pauletti, G.M., Xu, H., Zhang, J., Ewing, R.C., and Shi, D. (2015. )Photothermal Effects and Toxicity of Fe3O4 Nanoparticles via Near Infrared Laser Irradiation for Cancer Therapy.Mater. Sci. Eng. C, ,46 ,97-102

Li, H; Han, D; Pauletti, G M; Steckl, A J (2014. )Blood Coagulation Screening using a Paper-based Microfluidic Lateral Flow Device.Lab Chip, ,14 (20 ),4035-41

Gad, S.F., Fetih, G.N., Tous, S.S., and Pauletti, G.M. (2016. )Fabrication of Lipid-coated Chitosan Nanoparticles.Br. J. Pharm. Res., ,13 ,1-8

Abd Ellah, N., Taylor, L., Ayres, N., Pauletti, G.M., and Jones, H.N. (2015. )Development of Non-viral, Trophoblast-specific Gene Delivery for Placental Therapy.PLoS One, ,10 ,e0140879

Eraky, N., Mohamed, D.F., Attia, M.A., and Pauletti, G.M. (2015. )Hydrophobic Ion-Pairing of Low Molecular Weight Heparin with Cetyltrimethylammonium Bromide. Int. J. Pharma Sci. Res., ,6 ,558-565

Abd El Hamid, B., Soliman, G.M., Attia, M.A., and Pauletti, G.M. (2015. )An Ion Pairing Approach to Enhance Oral Bioavailability of Alendronate.J. Sci. Res. Reports, ,5 ,344-351

Abd Ellah, N., Potter, S.J., Taylor, L., Ayres, N., Elmahdy, M.M., Fetih, G.N., Ibrahim, E.A., and Pauletti, G.M. (2014. )Safety and Efficacy of Amine-containing Methacrylate Polymers as Non-viral Gene Delivery Vectors.J. Pharm. Tech. Drug Res., ,3 ,2

Abd Ellah, Taylor, L., Ayres, N., Elmahdy, M.M., Fetih, G.N., Ibrahim, E.A., and Pauletti, G.M. (2014. )Increased Transfection Efficiency of Lipid-Coated Methacrylate-Based Gene Delivery Systems. Int. J. Pharma Sci. Res., ,5 ,812-819

Sadat, M.E., Kaveh-Baghbadorani, M., Dunn, A.W., Wagner, H., Ewing, R.C., A.W., Zhang, J., Xu, H., Pauletti, G.M., Mast, D.B., and Shi, D. (2014. )Photoluminescence and the Effect of Structure in Fe3O4 Nanoparticles.Appl. Phys. Lett., ,105 ,091903

Sadat, M.E., Patel, R., Sookoor, J., Budko, S., Ewing, R.C., Zhang, J., Xu, H., Wang, Y., Pauletti, G.M., Mast, D.B., and Shi, D. (2014. )Effect of Spatial Confinement on Magnetic Hyperthermia via Dipolar Interactions in Fe3O4 Nanoparticles for Biomedical Applications.Mater. Sci. Eng. C, ,42 ,52-63

Book Chapter

Vromans, H and Pauletti, G.M. (2015 )Product Design Practical Pharmaceutics .(pp. 347-356), ).Royal Dutch Pharmacists Association,Amsterdam

Pauletti, G.M. (2009 )Physicochemcial and Biological Factors Affecting in vivo Performance of Drugs Handbook of Basic Pharmacokinetics .(pp. 67-80), ).American Pharmacists Association,Washington, DC

Pauletti, G.M. (2009 )Correlation of Clinical Response with Drug Disposition Handbook of Basic Pharmacokinetics .(pp. 357-366), ).American Pharmacists Association,Washington, DC

Honors and Awards

2015 Faculty Service Recognition Award (UC Winkle College of Pharmacy)

2015 Faculty Research Recognition Award (UC Winkle College of Pharmacy)

2015 Scientific Secretary (elected 2015-2019), International Pharmaceutical Federation (FIP)

2014 Vice President, FIP Board of Pharmaceutical Sciences (elected, 2014 – 2015)

2014 Faculty Service Recognition Award (UC Winkle College of Pharmacy)

Post Graduate Training and Education

Postdoctoral Fellow - Peptide Drug Delivery (1993 - 1997), The University of Kansas, Pharmaceutical Chemistry Department (Dr. Ronald T. Borchardt), ,

Keywords

Nanopharmaceutics, targeted drug delivery, membrane transport

Research and Practice Interests

Research interests focus on molecular pharmaceutics with an emphasis on membrane transport. Current activities include rational design, fabrication, and evaluation of innovative, non-viral nanocarriers as vectors for targeted drug delivery applications to improve therapeutic efficacy of small molecules, biotechnology drugs, and antitumor agents. Specifically, we apply phage display technology to identify unique peptide ligands that facilitate selective intracellular delivery and/or transepithelial transport of colloidal nanocarriers such as nanoparticles, liposomes, and niosomes. In addition, we are engaged in collaborative research projects with UC faculty in Material Science and Internal Medicine aimed at developing multifunctional nanocomposite structures suitable for drug delivery and non-invasive imaging applications.

Other Information

Current research team members include: Shindu Thomas (PhD student), Dr. Ezhilarasi Perumal (Postdoctoral Fellow), and Dr. Shiva Raju (Postdoctoral Fellow),

Contact Information

Office: Medical Sciences Building (MSB) 3109D
231 Albert Sabin Way
Ohio, 45267-0514
Phone: +1 (513) 558-4552
Fax: +1 (513) 558-3233
gm.pauletti@uc.edu