Mark A. Rance , PhD

B.Sc.: University of Waterloo Waterloo, Canada, 1976 (Physics)

Ph.D.: University of Guelph Guelph, Canada, 1981 (Physics/Biophysics)

The goal of this project is the development and evaluation of new, solution-state NMR methodologies for use in studies of the structure anddynamics of biomolecules. NMR spectroscopy is an extremely powerful toolfor the study of biomolecules in solution, due to its ability to providedetailed information at an atomic level. The extent to which NMRspectroscopy can be exploited for the structure determination ofbiomolecules, the investigation of intermolecular interactions in systemssuch as drugreceptor enzymesubstrate and antibodyantigen complexes, and the characterization of biomolecular dynamics depends on the abilityto manipulate the relevant NMR-active nuclei to yield the desiredinformation. The enormous impact that NMR spectroscopy has made duringthe last decade in the investigation of biomolecules and biophysicalprocesses is due largely to the continuing development of new or improvedtechniques for extracting useful data from the systems of interest. The proposal targets several general areas of solution-state NMRspectroscopy in which a number of specific goals will be pursued: 1Improvements in methodology for resonance assignment will be sought. Inparticular, the development of enhanced sensitivity, relaxation- compensated techniques for performing coherence transfer experiments willbe pursued. 2 Rotating-frame relaxation experiments for studyingexchange processes in the microsecond to millisecond time regime will beinvestigated. The theoretical foundation for such experiments will belaid in order toopen up new windows on biomolecular dynamics. Exchangeeffects in coherence transfer experiments will also be explored with thegoal of designing more robust methods in the presence of exchange. 3Heteronuclear cross-polarization experiments will be developed which willoptimize sensitivity for several important applications. 4 Muchexcitement has been generated in the biomolecular NMR community over therecent introduction of higher field 750 MHz proton frequencyspectrometers. Significant improvements in spectral resolution andinherent sensitivity are anticipated for most nuclei. However, significant challenges will also be faced in realizing the fun potentialof the new instrumentation. A thorough investigation will be conductedof various aspects of NMR spectroscopy at very high magnetic fields inthe context of biomolecular NMR applications. 5 Novel experiments formeasuring fast proton exchange rates and heteronuclear NOEs will bedeveloped. The relative merits of single- versus triple-axis magneticfield gradients will be explored, and protocols will be investigated tofacilitate the design, set-up and execution of optimized gradient- enhanced NMR experiments. 6 Our existing simulation software will beimproved to allow a determination of nuclear spin dynamics under theinfluence of radio-frequency irradiation, spin relaxation and exchangeprocesses. The correct interpretation of NMR data, and therefore theveracity of the resulting conclusions, depends critically on having notjust some intuitive insight into the sophisticated NMR experimentsemployed but also a detailed understanding of the underlying physics.

1985 -1990 Assistant Member, NMR methodology development and studies of the structure and dynamics of proteins and nucleic acids, The Scripps Research Institute, La Jolla, California

1990 -1995 Associate Member, The Scripps Research Institute, La Jolla, California

1996 -2006 Associate Professor, University of Cincinnati, Cincinnati, Ohio

2006 -To Present Professor, University of Cincinnati, Cincinnati, Ohio

Grant: #R01-GM-063855 Investigators:Rance, Mark 05-01-2003 -01-31-2009 National Institute of General Medical Sciences Protein Dynamics in Homeodomain/DNA Complexes Role:PI $1,247,539.00 Closed Level:Federal

Grant: #1-S10-RR-019077-01-A0-S0-E0 Investigators:Rance, Mark 05-01-2004 -12-31-2005 National Center for Research Resources Cryoprobe for 800 MHz NMR Spectrometer Role:PI $345,443.00 Closed Level:Federal

Investigators:Rance, Mark 06-01-2003 -05-31-2004 Ohio Board of Regents Ohio NMR Consortium Role:PI $114,500.00 Closed Level:State of Ohio

Grant: #2001-1792-01 / R01 GM65156 Investigators:Rance, Mark 02-01-2003 -01-31-2008 National Institute of General Medical Sciences Structural Studies of Bacterial Competence Proteins Role:PI $353,278.00 Closed Level:Federal

Grant: #5-R01-GM-40089-11-A0-S0-E0 Investigators:Rance, Mark 01-24-1996 -12-31-1999 National Institute of General Medical Sciences Methodology Development for NMR Studies of Biomolecules Role:PI $651,413.00 Closed Level:Federal

Grant: #P&G-27-01-A0-S0-E0 Investigators:Rance, Mark 09-01-1998 -08-31-1999 Procter & Gamble Company Identification of Ligands to Target Molecules by NMR Spectroscopy Role:PI $26,000.00 Closed Level:Industry

Grant: #5-R01-GM-40089-15-A0-S0-E0 Investigators:Rance, Mark 02-01-2000 -01-31-2005 National Institute of General Medical Sciences Methodology Development for Nuclear Magnetic Resonance Studies of Biomolecules Role:PI $867,494.00 Closed Level:Federal

Grant: #R01 HL083334 Investigators:Finley, Natosha; Howarth, Jack; Rance, Mark; Rosevear, Paul 06-15-2007 -04-30-2011 National Heart, Lung and Blood Institute Structural Mechanisms Modulating Contraction in Normal and Diseased Heart Role:Collaborator $1,532,112.00 Closed Level:Federal

Grant: #R01 GM063855 Investigators:Rance, Mark 09-01-2009 -07-31-2014 National Institute of General Medical Sciences NMR Studies of Protein Side-Chain Dynamics Role:PI $1,329,425.00 Active Level:Federal

Grant: #1 S10 RR027755-01 Investigators:Herr, Andrew; Rance, Mark; Rosevear, Paul 12-10-2009 -12-09-2010 National Center for Research Resources 800 MHz NMR Console Replacement Role:PI $358,323.00 Closed Level:Federal

Grant: #USDA-NACA 58-6034-0-003 Investigators:Rance, Mark 03-01-2020 -09-30-2021 Department of Agriculture Assessment of Metabolites in Soil Treated with Anaerobic Soil Disinfestation Role:PI $15,000.00 Awarded Level:Federal

Peer Reviewed Publications

Rance, M; Sørensen, O W; Bodenhausen, G; Wagner, G; Ernst, R R; Wüthrich, K (2012. ) Improved spectral resolution in COSY (1)H NMR spectra of proteins via double quantum filtering. 1983.Biochemical and biophysical research communications, , 425 (3 ) ,527-33 More Information

Doerdelmann, Thomas; Kojetin, Douglas J; Baird-Titus, Jamie M; Rance, Mark (2012. ) ¹H, ¹³C and ¹?N chemical shift assignments for the human Pitx2 homeodomain in complex with a 22-base hairpin DNA.Biomolecular NMR assignments, , 6 (1 ) ,79-81 More Information

Doerdelmann, Thomas; Kojetin, Douglas J; Baird-Titus, Jamie M; Solt, Laura A; Burris, Thomas P; Rance, Mark (2012. ) Structural and biophysical insights into the ligand-free Pitx2 homeodomain and a ring dermoid of the cornea inducing homeodomain mutant.Biochemistry, , 51 (2 ) ,665-76 More Information

Hughes, Travis S; Chalmers, Michael J; Novick, Scott; Kuruvilla, Dana S; Chang, Mi Ra; Kamenecka, Theodore M; Rance, Mark; Johnson, Bruce A; Burris, Thomas P; Griffin, Patrick R; Kojetin, Douglas J (2012. ) Ligand and receptor dynamics contribute to the mechanism of graded PPAR? agonism.Structure (London, England : 1993), , 20 (1 ) ,139-50 More Information

Doerdelmann, Thomas; Kojetin, Douglas J; Baird-Titus, Jamie M; Rance, Mark (2011. ) 1H, 13C and 15N chemical shift assignments for the human Pitx2 homeodomain and a R24H homeodomain mutant.Biomolecular NMR assignments, , 5 (1 ) ,105-7 More Information

Refaei, Mary Anne; Combs, Al; Kojetin, Douglas J; Cavanagh, John; Caperelli, Carol; Rance, Mark; Sapitro, Jennifer; Tsang, Pearl (2011. ) Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy.Journal of biomolecular NMR, , 49 (1 ) ,3-7 More Information

Kojetin, Douglas J; McLaughlin, Patrick D; Thompson, Richele J; Dubnau, David; Prepiak, Peter; Rance, Mark; Cavanagh, John (2009. ) Structural and motional contributions of the Bacillus subtilis ClpC N-domain to adaptor protein interactions.Journal of molecular biology, , 387 (3 ) ,639-52 More Information

Trbovic, Nikola; Cho, Jae-Hyun; Abel, Robert; Friesner, Richard A; Rance, Mark; Palmer, Arthur G (2009. ) Protein side-chain dynamics and residual conformational entropy.Journal of the American Chemical Society, , 131 (2 ) ,615-22 More Information

Sullivan, Daniel M; Bobay, Benjamin G; Kojetin, Douglas J; Thompson, Richele J; Rance, Mark; Strauch, Mark A; Cavanagh, John (2008. ) Insights into the nature of DNA binding of AbrB-like transcription factors.Structure (London, England : 1993), , 16 (11 ) ,1702-13 More Information

Kojetin, Douglas J; McLaughlin, Patrick D; Thompson, Richele J; Venters, Ronald A; Rance, Mark; Cavanagh, John (2007. ) NMR assignment of the N-terminal repeat domain of Bacillus subtilis ClpC.Biomolecular NMR assignments, , 1 (2 ) ,163-5 More Information

Showalter, Scott A; Johnson, Eric; Rance, Mark; Brüschweiler, Rafael (2007. ) Toward quantitative interpretation of methyl side-chain dynamics from NMR by molecular dynamics simulations.Journal of the American Chemical Society, , 129 (46 ) ,14146-7 More Information

Johnson, Eric; Palmer, Arthur G; Rance, Mark (2007. ) Temperature dependence of the NMR generalized order parameter.Proteins, , 66 (4 ) ,796-803 More Information

Johnson, Eric; Chazin, Walter J; Rance, Mark (2006. ) Effects of calcium binding on the side-chain methyl dynamics of calbindin D9k: a 2H NMR relaxation study.Journal of molecular biology, , 357 (4 ) ,1237-52 More Information

Baird-Titus, Jamie M; Clark-Baldwin, Kimber; Dave, Vrushank; Caperelli, Carol A; Ma, Jun; Rance, Mark (2006. ) The solution structure of the native K50 Bicoid homeodomain bound to the consensus TAATCC DNA-binding site.Journal of molecular biology, , 356 (5 ) ,1137-51 More Information

Chaney, Beth A; Clark-Baldwin, Kimber; Dave, Vrushank; Ma, Jun; Rance, Mark (2005. ) Solution structure of the K50 class homeodomain PITX2 bound to DNA and implications for mutations that cause Rieger syndrome.Biochemistry, , 44 (20 ) ,7497-511 More Information

Wang, Chunyu; Karpowich, Nathan; Hunt, John F; Rance, Mark; Palmer, Arthur G (2004. ) Dynamics of ATP-binding cassette contribute to allosteric control, nucleotide binding and energy transduction in ABC transporters.Journal of molecular biology, , 342 (2 ) ,525-37 More Information

Butterwick, Joel A; Patrick Loria, J; Astrof, Nathan S; Kroenke, Christopher D; Cole, Roger; Rance, Mark; Palmer, Arthur G (2004. ) Multiple time scale backbone dynamics of homologous thermophilic and mesophilic ribonuclease HI enzymes.Journal of molecular biology, , 339 (4 ) ,855-71 More Information

Bramham, Janice; Rance, Mark; Thai, Chuong-Thu; Uhrín, Dusan; Assa-Munt, Nuria; Ogata, Ronald T; Barlow, Paul N (2004. ) 1H, 15N and 13C resonance assignments of the C345C domain of the complement component C5.Journal of biomolecular NMR, , 29 (2 ) ,217-8 More Information

Massi, Francesca; Johnson, Eric; Wang, Chunyu; Rance, Mark; Palmer, Arthur G (2004. ) NMR R1 rho rotating-frame relaxation with weak radio frequency fields.Journal of the American Chemical Society, , 126 (7 ) ,2247-56 More Information

Wang, Chunyu; Rance, Mark; Palmer, Arthur G (2003. ) Mapping chemical exchange in proteins with MW > 50 kD.Journal of the American Chemical Society, , 125 (30 ) ,8968-9 More Information

Wang, Chunyu; Hunt, John F; Rance, Mark; Palmer, Arthur G (2002. ) 1H, 15N and 13C backbone assignment of MJ1267, an ATP-binding cassette. Journal of biomolecular NMR, , 24 (2 ) ,167-8

Xue, Weiling; Siner, Angela; Rance, Mark; Jayasimhulu, Koka; Talaska, Glenn; Warshawsky, David (2002. ) A metabolic activation mechanism of 7H-dibenzo[c,g]carbazole via o-quinone. Part 2: covalent adducts of 7H-dibenzo[c,g]carbazole-3,4-dione with nucleic acid bases and nucleosides. Chemical research in toxicology, , 15 (7 ) ,915-21

Christodoulou, John; Hu, Haitao; Chung, John; Rance, Mark; Harper, Jeffrey F; Chazin, Walter J (2002. ) 1H, 15N and 13C assignments of the regulatory domains of calcium-dependent protein kinase (CDPK). Journal of biomolecular NMR, , 23 (3 ) ,249-50

Sheng, Wanyun; Rance, Mark; Liao, Xiubei (2002. ) Structure comparison of two conserved HNF-3/fkh proteins HFH-1 and genesis indicates the existence of folding differences in their complexes with a DNA binding sequence. Biochemistry, , 41 (10 ) ,3286-93

Abbott, M B; Gaponenko, V; Abusamhadneh, E; Finley, N; Li, G; Dvoretsky, A; Rance, M; Solaro, R J; Rosevear, P R (2000. ) Regulatory domain conformational exchange and linker region flexibility in cardiac troponin C bound to cardiac troponin I.The Journal of biological chemistry, , 275 (27 ) ,20610-7 More Information

Mäler, L; Blankenship, J; Rance, M; Chazin, W J (2000. ) Site-site communication in the EF-hand Ca2+-binding protein calbindin D9k.Nature structural biology, , 7 (3 ) ,245-50 More Information

Meininger, ; Rance, ; Starovasnik, ; Fairbrother, ; Skelton, (2000. ) Characterization of the binding interface between the E-domain of staphylococcal protein A and an antibody Fv-fragment .Biochemistry, , 39 (6 ) ,1541

Cipollo, J F; Trimble, R B; Rance, M; Cavanagh, J (2000. ) Two-dimensional relayed-rotating-frame overhauser spectroscopy (1)H NMR experiments for the selective identification of 1,2-glycosidic linkages in polysaccharides.Analytical biochemistry, , 278 (1 ) ,52-8 More Information

Meininger, D P; Rance, M; Starovasnik, M A; Fairbrother, W J; Skelton, N J (2000. ) Characterization of the binding interface between the E-domain of Staphylococcal protein A and an antibody Fv-fragment. Biochemistry, , 39 (1 ) ,26-36

Loria, J P; Rance, M; Palmer, A G (1999. ) Transverse-relaxation-optimized (TROSY) gradient-enhanced triple-resonance NMR spectroscopy.Journal of magnetic resonance (San Diego, Calif. : 1997), , 141 (1 ) ,180-4 More Information

Loria, J P; Rance, M; Palmer, A G (1999. ) A TROSY CPMG sequence for characterizing chemical exchange in large proteins.Journal of biomolecular NMR, , 15 (2 ) ,151-5 More Information

Finley, N; Abbott, M B; Abusamhadneh, E; Gaponenko, V; Dong, W; Gasmi-Seabrook, G; Howarth, J W; Rance, M; Solaro, R J; Cheung, H C; Rosevear, P R (1999. ) NMR analysis of cardiac troponin C-troponin I complexes: effects of phosphorylation. FEBS letters, , 453 (1-2 ) ,107-12

Gaponenko, V; Abusamhadneh, E; Abbott, M B; Finley, N; Gasmi-Seabrook, G; Solaro, R J; Rance, M; Rosevear, P R (1999. ) Effects of troponin I phosphorylation on conformational exchange in the regulatory domain of cardiac troponin C. The Journal of biological chemistry, , 274 (24 ) ,16681-4

Boulat, B; Epstein, D M; Rance, M (1999. ) Selective injection of magnetization by slow chemical exchange in NMR.Journal of magnetic resonance (San Diego, Calif. : 1997), , 138 (2 ) ,268-76 More Information

Rance, M; Loria, J P; Palmer AG3rd, (1999. ) Sensitivity improvement of transverse relaxation-optimized spectroscopy. Journal of magnetic resonance (San Diego, Calif. : 1997), , 136 (1 ) ,92-101

Kupce, E; Schmidt, P; Rance, M; Wagner, G (1998. ) Adiabatic mixing in the liquid state.Journal of magnetic resonance (San Diego, Calif. : 1997), , 135 (2 ) ,361-7 More Information

Feher, V A; Zapf, J W; Hoch, J A; Whiteley, J M; McIntosh, L P; Rance, M; Skelton, N J; Dahlquist, F W; Cavanagh, J (1997. ) High-resolution NMR structure and backbone dynamics of the Bacillus subtilis response regulator, Spo0F: implications for phosphorylation and molecular recognition.Biochemistry, , 36 (33 ) ,10015-25 More Information

Lee, L K; Rance, M; Chazin, W J; Palmer, A G (1997. ) Rotational diffusion anisotropy of proteins from simultaneous analysis of 15N and 13C alpha nuclear spin relaxation. Journal of biomolecular NMR, , 9 (3 ) ,287-98

Krishnan, V V; Rance, M (1997. ) Calculation of coherence-transfer behavior under planar versus isotropic mixing Hamiltonians and application to heteronuclear J cross-polarization experiments in solution-state NMR spectroscopy.Journal of magnetic resonance (San Diego, Calif. : 1997), , 124 (1 ) ,205-9 More Information

Tsang, P; Rance, M (1996. ) Some practical aspects of double-resonance techniques in solution-state NMR studies of high-molecular-weight systems. Journal of magnetic resonance. Series B, , 111 (2 ) ,135-48

Boulat, B; Rance, M (1996. ) Selective transfer of magnetization by incoherent processes in nuclear magnetic resonance spectroscopy. Journal of magnetic resonance. Series B, , 110 (3 ) ,288-97

Fairbrother, W J; Palmer, A G; Rance, M; Reizer, J; Saier, M H; Wright, P E (1992. ) Assignment of the aliphatic 1H and 13C resonances of the Bacillus subtilis glucose permease IIA domain using double- and triple-resonance heteronuclear three-dimensional NMR spectroscopy. Biochemistry, , 31 (18 ) ,4413-25

Chen, S M; Leupin, W; Rance, M; Chazin, W J (1992. ) Two-dimensional NMR studies of d(GGTTAATGCGGT).d(ACCGCATTAACC) complexed with the minor groove binding drug SN-6999. Biochemistry, , 31 (18 ) ,4406-13

Tsang, P; Rance, M; Fieser, T M; Ostresh, J M; Houghten, R A; Lerner, R A; Wright, P E (1992. ) Conformation and dynamics of an Fab'-bound peptide by isotope-edited NMR spectroscopy. Biochemistry, , 31 (15 ) ,3862-71

Palmer, A G; Fairbrother, W J; Cavanagh, J; Wright, P E; Rance, M (1992. ) Improved resolution in three-dimensional constant-time triple resonance NMR spectroscopy of proteins. Journal of biomolecular NMR, , 2 (1 ) ,103-8

Chazin, W J; Rance, M; Chollet, A; Leupin, W (1991. ) Comparative NMR analysis of the decadeoxynucleotide d-(GCATTAATGC)2 and an analogue containing 2-aminoadenine. Nucleic acids research, , 19 (20 ) ,5507-13

Tsang, P; Rance, M; Wright, P E (1991. ) Isotope-edited nuclear magnetic resonance studies of Fab-peptide complexes. Methods in enzymology, , 203 ,241-61

Rance, M; Chazin, W J; Dalvit, C; Wright, P E (1989. ) Multiple-quantum nuclear magnetic resonance. Methods in enzymology, , 176 ,114-34

Chazin, W J; Rance, M; Wright, P E (1988. ) Complete assignment of the 1H nuclear magnetic resonance spectrum of French bean plastocyanin. Application of an integrated approach to spin system identification in proteins. Journal of molecular biology, , 202 (3 ) ,603-22

Dyson, H J; Rance, M; Houghten, R A; Wright, P E; Lerner, R A (1988. ) Folding of immunogenic peptide fragments of proteins in water solution. II. The nascent helix. Journal of molecular biology, , 201 (1 ) ,201-17

Dyson, H J; Rance, M; Houghten, R A; Lerner, R A; Wright, P E (1988. ) Folding of immunogenic peptide fragments of proteins in water solution. I. Sequence requirements for the formation of a reverse turn. Journal of molecular biology, , 201 (1 ) ,161-200

Chazin, W J; Rance, M; Wright, P E (1987. ) Complete assignment of lysine resonances in 1H NMR spectra of proteins as probes of surface structure and dynamics. FEBS letters, , 222 (1 ) ,109-14

Chazin, W J; Wüthrich, K; Hyberts, S; Rance, M; Denny, W A; Leupin, W (1986. ) 1H nuclear magnetic resonance assignments for d-(GCATTAATGC)2 using experimental refinements of established procedures. Journal of molecular biology, , 190 (3 ) ,439-53

Rance, M; Dalvit, C; Wright, P E (1985. ) Simplification of 1H NMR spectra of proteins by one-dimensional multiple quantum filtration. Biochemical and biophysical research communications, , 131 (3 ) ,1094-102

Rance, M; Sørensen, O W; Bodenhausen, G; Wagner, G; Ernst, R R; Wüthrich, K (1983. ) Improved spectral resolution in cosy 1H NMR spectra of proteins via double quantum filtering. Biochemical and biophysical research communications, , 117 (2 ) ,479-85

Rance, M; Jeffrey, K R; Tulloch, A P; Butler, K W; Smith, I C (1982. ) Effects of cholesterol on the orientational order of unsaturated lipids in the membranes of acholeplasma laidlawii. A 2H-NMR study. Biochimica et biophysica acta, , 688 (1 ) ,191-200

Rance, M; Jeffrey, K R; Tulloch, A P; Butler, K W; Smith, I C (1980. ) Orientational order of unsaturated lipids in the membranes of Acholeplasma laidlawii as observed by 2H-NMR. Biochimica et biophysica acta, , 600 (2 ) ,245-62

Siminovitch, D J; Rance, M; Jeffrey, K R (1980. ) The use of wide-line [14N]nitrogen NMR as a probe in model membranes. FEBS letters, , 112 (1 ) ,79-82

O.W. Sørensen, M. Rance and R.R. Ernst (1984. ) z Filters for purging phase- or multiplet-distorted spectra .J. Magn. Reson., , 56 ,527 -534

M. Rance, I.C.P. Smith and H.C. Jarrell (1983. ) The effect of headgroup class on the conformation of membrane lipids in Acholeplasma laidlawii: A 2H NMR Study .Chem. Phys. Lipids, , 32 ,57 -71

M. Rance and R.A. Byrd (1983. ) Obtaining high-fidelity spin-1/2 powder spectra in anisotropic media: Phase-cycled Hahn echo spectroscopy .J. Magn. Reson., , 52 ,221 -240

D.J. Siminovitch, M. Rance, K.R. Jeffrey and M.F. Brown (1984. ) The quadrupolar spectrum of a spin I=1 in a lipid bilayer in the presence of paramagnetic ions .J. Magn. Reson., , 58 ,62 -75

M. Rance, G. Wagner, O.W. Sørensen, K. Wüthrich and R.R. Ernst (1984. ) Application of ω1-decoupled 2D correlation spectra to the study of proteins .J. Magn. Reson., , 59 ,250 -261

G. Bodenhausen, G. Wagner, M. Rance, O.W. Sørensen, K. Wüthrich and R.R. Ernst (1984. ) Longitudinal two-spin order in 2D exchange spectroscopy (NOESY) .J. Magn. Reson., , 59 ,542 -550

M. Rance, O.W. Sørensen, W. Leupin, H. Kogler, K. Wüthrich and R.R. Ernst (1985. ) Uniform excitation of a multiple quantum coherence. Application to two-dimensional double quantum spectroscopy .J. Magn. Reson., , 61 ,67 -80

W. Denk, G. Wagner, M. Rance and K. Wüthrich (1985. ) Combined suppression of diagonal peaks and t1 ridges in two-dimensional nuclear Overhauser enhancement spectra (NOESY) .J. Magn. Reson., , 62 ,350 -355

M. Rance, G. Bodenhausen, G. Wagner, K. Wüthrich and R.R. Ernst (1985. ) A systematic approach to the suppression of J cross peaks in 2D exchange and 2D NOE spectroscopy .J. Magn. Reson., , 62 ,497 -510

G. Wagner, G. Bodenhausen, N. Müller, M. Rance, O.W. Sørensen, R.R. Ernst and K. Wüthrich (1985. ) Exchange of two-spin order in nuclear magnetic resonance: separation of exchange and cross-relaxation processes .J. Am. Chem. Soc., , 107 ,6440 -6446

M. Rance and P.E. Wright (1986. ) Analysis of 1H NMR spectra of proteins using multiple quantum coherence .J. Magn. Reson., , 66 ,372 -378

M. Rance and P.E. Wright (1986. ) Selection rule violations in multiple quantum NMR spectroscopy .Chem. Phys. Lett., , 124 ,572 -575

C. Dalvit, M. Rance and P.E. Wright (1986. ) Differentiation of direct and remote connectivities in phase-sensitive double quantum spectra of proteins by variation of the multiple quantum excitation period .J. Magn. Reson., , 69 ,356 -361

C. Dalvit, P.E. Wright and M. Rance (1987. ) Detection of long-range couplings in proteins via double quantum spectroscopy. Connection of aliphatic and imidazole resonances of histidine residues .J. Magn. Reson., , 71 ,539 -543

M. Rance, P.E. Wright, B.A. Messerle and L.D. Field (1987. ) Site-selective observation of nuclear Overhauser effects in proteins via isotopic labeling .J. Am. Chem. Soc., , 109 ,1591 -1593

M. Rance (1987. ) Improved techniques for homonuclear rotating-frame and isotropic mixing experiments .J. Magn. Reson., , 74 ,557 -564

M. Rance (1988. ) Intramolecular exchange effects in multiple quantum spectroscopy .J. Am. Chem. Soc., , 110 ,1973 -1974

W.J. Chazin, M. Rance and P.E. Wright (1988. ) Complete assignment of the 1H nuclear magnetic resonance spectrum of French bean plastocyanin. Application of an integrated approach to spin system identification in proteins .J. Mol. Biol., , 202 ,603 -622

M. Rance (1989. ) Sign reversal of resonances via isotropic mixing in NMR spectroscopy .Chem. Phys. Lett., , 154 ,242 -247

John Cavanagh, Walter J. Chazin and Mark Rance (1990. ) The time dependence of coherence transfer in homonuclear isotropic mixing experiments .J. Magn. Reson., , 87 ,110 -131

Mark Rance and John Cavanagh (1990. ) RF phase coherence in rotating frame NMR experiments in isotropic solutions .J. Magn. Reson., , 87 ,363 -371

John Cavanagh and Mark Rance (1990. ) A combined two-dimensional relayed-NOESY/TOCSY experiment .J. Magn. Reson., , 87 ,408 -414

John Cavanagh and Mark Rance (1990. ) Sensitivity improvement in isotropic mixing (TOCSY) experiments .J. Magn. Reson., , 88 ,72 -85

Pearl Tsang, Peter E. Wright and Mark Rance (1990. ) Signal suppression in the frequency domain to remove undesirable resonances with dispersive lineshapes .J. Magn. Reson., , 88 ,210 -215

Pearl Tsang, Peter E. Wright and Mark Rance (1990. ) Specific deuteration strategy for enhancing direct nuclear Overhauser effects in high molecular weight complexes .J. Am. Chem. Soc., , 112 ,8183 -8185

John Cavanagh, Arthur G. Palmer III, Peter E. Wright and Mark Rance (1991. ) Sensitivity improvement in proton-detected two-dimensional heteronuclear relay spectroscopy .J. Magn. Reson., , 91 ,429 -436

Arthur G. Palmer III, John Cavanagh, Peter E. Wright and Mark Rance (1991. ) Sensitivity improvement in proton-detected two-dimensional heteronuclear correlation NMR spectroscopy .J. Magn. Reson., , 93 ,151 -170

Arthur G. Palmer III, Mark Rance and Peter E. Wright (1991. ) Intramolecular motions of a zinc finger DNA-binding domain from Xfin characterized by proton-detected natural abundance 13C heteronuclear NMR spectroscopy .J. Am. Chem. Soc., , 113 ,4371 -4380

Arthur G. Palmer III, Peter E. Wright and Mark Rance (1991. ) Measurement of relaxation rate constants for methyl groups by proton-detected heteronuclear NMR spectroscopy .Chem. Phys. Lett., , 185 ,41 -46

Arthur G. Palmer, III, Nicholas J. Skelton, Walter J. Chazin, Peter E. Wright and Mark Rance (1992. ) Suppression of the effects of cross-correlation between dipolar and anisotropic chemical shift relaxation mechanisms in the measurement of spin-spin relaxation rates .Mol. Phys., , 75 ,699 -711

Arthur G. Palmer, III, John Cavanagh, R. Andrew Byrd and Mark Rance (1992. ) Sensitivity improvement in three-dimensional heteronuclear correlation NMR spectroscopy .J. Magn. Reson., , 96 ,416 -424

Arthur G. Palmer III, Wayne J. Fairbrother, John Cavanagh, Peter E. Wright and Mark Rance (1992. ) Improved resolution in three-dimensional constant-time triple resonance NMR spectroscopy of proteins .J. Biomol. NMR, , 2 ,103 -108

John Cavanagh and Mark Rance (1992. ) Suppression of cross-relaxation effects in TOCSY spectra via a modified DIPSI-2 mixing sequence .J. Magn. Reson., , 96 ,670 -678

Arthur G. Palmer, III, Remo A. Hochstrasser, David P. Millar, Mark Rance and Peter E. Wright (1993. ) Characterization of amino acid side chain dynamics in a zinc finger peptide using 13C NMR spectroscopy and time-resolved fluorescence spectroscopy .J. Am. Chem. Soc., , 115 ,6333 -6345

Nicholas J. Skelton, Arthur G. Palmer, III, Mikael Akke, Johan Kördel, Mark Rance and Walter J. Chazin (1993. ) Practical aspects of two-dimensional proton-detected 15N spin relaxation measurements .J. Magn. Reson., , B 102 ,253 -264

Mark Rance (1994. ) Sensitivity improvement in multi-dimensional NMR spectroscopy .Bull. Magn. Reson., , 16 ,54 -67

Benoit Boulat and Mark Rance (1994. ) Algebraic formulation of the product operator formalism in the numerical simulation of the dynamical behavior of multispin systems .Mol. Phys., , 83 ,1021 -1039

Benoit Boulat and Mark Rance (1994. ) Monitoring of slow conformational exchange by doubly selective irradiation in nuclear magnetic resonance spectroscopy .J. Chem. Phys., , 101 ,7273 -7282

V.V. Krishnan and Mark Rance (1995. ) Influence of chemical exchange among homonuclear spins in heteronuclear coherence transfer experiments in liquids .J. Magn. Reson., , A 116 ,97 -106

Benoit Boulat, Igor Najfeld, and Mark Rance (1996. ) A theoretical analysis of the synchronous nutation experiment .J. Magn. Reson., , A 120 ,223 -230

Christopher D. Kroenke, J. Patrick Loria, Larry K. Lee, Mark Rance, and Arthur G. Palmer, III (1998. ) Longitudinal and transverse 1H−15N dipolar/15N chemical shift anisotropy relaxation interference: Unambiguous determination of rotational diffusion tensors and chemical exchange effects in biological macromolecules .J. Am. Chem. Soc., , 120 ,7905 -7915

Mark R. Hansen, Mark Rance, and Arthur Pardi (1998. ) Observation of long-range 1H−1H distances in solution by dipolar coupling interactions .J. Am. Chem. Soc., , 120 ,11210 -11211

J. Patrick Loria, Mark Rance and Arthur G. Palmer III (1999. ) A relaxation-compensated Carr-Purcell-Meiboom-Gill sequence for characterizing chemical exchange by NMR spectroscopy .J. Am. Chem. Soc., , 121 ,2331 -2332

Christopher D. Kroenke, Mark Rance, and Arthur G. Palmer III (1999. ) The variability of the 15N chemical shift anisotropy in Escherichia coli ribonuclease H in solution .J. Am. Chem. Soc., , 121 ,10119 -10125

Li, Ying; Rance, Mark; Palmer, Arthur G (2014. ) Rotation operator propagators for time-varying radiofrequency pulses in NMR spectroscopy: applications to shaped pulses and pulse trains.Journal of magnetic resonance (San Diego, Calif. : 1997), , 248 ,105-14 More Information

Kuo, Shiu-Ming; Wang, Li-Yuan; Yu, Siyuan; Campbell, Christine E; Valiyaparambil, Sujith A; Rance, Mark; Blumenthal, Kenneth M (2013. ) The N-terminal basolateral targeting signal unlikely acts alone in the differential trafficking of membrane transporters in MDCK cells.Biochemistry, , 52 (30 ) ,5103-16 More Information

Kesarwani, Meenu; Huber, Erika; Kincaid, Zachary; Evelyn, Chris R; Biesiada, Jacek; Rance, Mark; Thapa, Mahendra B; Shah, Neil P; Meller, Jarek; Zheng, Yi; Azam, Mohammad (2015. ) Targeting substrate-site in Jak2 kinase prevents emergence of genetic resistance.Scientific reports, , 5 ,14538 More Information

Kojetin, Douglas J; Matta-Camacho, Edna; Hughes, Travis S; Srinivasan, Sathish; Nwachukwu, Jerome C; Cavett, Valerie; Nowak, Jason; Chalmers, Michael J; Marciano, David P; Kamenecka, Theodore M; Shulman, Andrew I; Rance, Mark; Griffin, Patrick R; Bruning, John B; Nettles, Kendall W (2015. ) Structural mechanism for signal transduction in RXR nuclear receptor heterodimers.Nature communications, , 6 ,8013 More Information

Koss, Hans; Rance, Mark; Palmer, Arthur G (2017. ) General expressions for R1? relaxation for N-site chemical exchange and the special case of linear chains.Journal of magnetic resonance (San Diego, Calif. : 1997), , 274 ,36-45 More Information

Thapa, Mahendra; Johnson, Eric; Rance, Mark (2019. ) Effect of monovalent ion binding on molecular dynamics of the S100-family calcium-binding protein calbindin D9k.Journal of computational chemistry, , 40 (22 ) ,1936-1945 More Information

de Vera, Ian Mitchelle S; Munoz-Tello, Paola; Zheng, Jie; Dharmarajan, Venkatasubramanian; Marciano, David P; Matta-Camacho, Edna; Giri, Pankaj Kumar; Shang, Jinsai; Hughes, Travis S; Rance, Mark; Griffin, Patrick R; Kojetin, Douglas J (2019. ) Defining a Canonical Ligand-Binding Pocket in the Orphan Nuclear Receptor Nurr1.Structure (London, England : 1993), , 27 (1 ) ,66-77.e5 More Information

Koss, Hans; Rance, Mark; Palmer, Arthur G (2018. ) General Expressions for Carr-Purcell-Meiboom-Gill Relaxation Dispersion for N-Site Chemical Exchange.Biochemistry, , 57 (31 ) ,4753-4763 More Information

Baird-Titus, Jamie M; Thapa, Mahendra; Doerdelmann, Thomas; Combs, Kelly A; Rance, Mark (2018. ) Lysine Side-Chain Dynamics in the Binding Site of Homeodomain/DNA Complexes As Observed by NMR Relaxation Experiments and Molecular Dynamics Simulations.Biochemistry, , 57 (19 ) ,2796-2813 More Information

Hsu, Andrew; O'Brien, Paul A; Bhattacharya, Shibani; Rance, Mark; Palmer, Arthur G (2018. ) Enhanced spectral density mapping through combined multiple-field deuterium 13CH2D methyl spin relaxation NMR spectroscopy.Methods (San Diego, Calif.), , 138-139 ,76-84 More Information

Rance, Mark (2017. ) Exploring a New Approach for Discovery of Conformational Heterogeneity in Homeodomain-DNA Complexes.Biochemistry, , 56 (38 ) ,5033-5034 More Information

Published Books

John Cavanagh, Wayne J. Fairbrother, Arthur G. Palmer III, Mark Rance and Nicholas J. Skelton (2006. ) Protein NMR Spectroscopy. Principles and Practice, 2nd Edition .Elsevier (Co-Author)

1981-1982 Research Associate, research in the dynamics of biological membranes, National Research Council of Canada, , Ottawa, Canada

1982-1984 postdoctoral fellow, development of new NMR methodologies for studies of the structure and dynamics of biomolecules such as proteins and nucleic acids, ETH, , Zurich, Switzerland

1984-1985 senior research associate, NMR studies of protein structure and dynamics, The Scripps Research Institute, , La Jolla, California

Solution,Biophysics,Magnetic Field,Protein Structure,Quantum Chemistry,Method Development,Molecular Dynamics,Structural Biology,Computer Simulation,Nucleic Acid Structure,Computer Program Software,Intermolecular Interaction,Biomedical Equipment Development,Nonclinical Biomedical Equipment,Computer System Design Evaluation,Nuclear Magnetic Resonance Spectroscopy