Mary Risinger

Mary A Risinger

Asst Professor - Research

Procter Hall

229

CON AD for Nursing Research - 0038

229 Procter Hall

Professional Summary

Dr. Risinger studies Hereditary Hemolytic Anemias (HHAs), including
red blood cell (RBC) membrane disorders and Sickle Cell Disease
(SCD), with particular emphasis on the role of proteins controlling
RBC hydration. In addition to contributing to our understanding of
the function of proteins that control cell hydration in normal RBCs
and their pathological contribution in HHAs, these studies may
provide information on genetic modifiers for SCD and help suggest
additional therapeutic targets for treatment.

Education

BSN, Marycrest College Davenport, IA, 1972 (Nursing)

MA, University of Iowa Iowa City, IA, 1976 (Medical-Surgical Nursing, Basic Sciences)

PhD, University of Iowa Iowa City, IA, 1983 (Anatomy and Cell Biology)

Publications

Published Abstracts

Gupta, S., Baskin, J., Kalfa, T., Risinger, M., and Zhang, K. (2016. )Hereditary hemolytic anemia due to red cell monovalent cation leak in a patient with a novel band 3 mutation. Pediatr. Blood Cancer, 63 ,S58-S59

Risinger, M., Dagaonkar, N., Christakopoulos, G., Liu, J., Seu, K.G., Konstantinidis, D., Quinn, C., Chonat, S., Joiner, C.H., Zhang, K., Kalfa, T.A. (2016. )Cellular hydration and oxidation as phenotype modifiers in sickle cell anemia. Blood, 128 ,2446

Lesmana, H., Christakopoulos, G.E., Seu, K.G., Risinger, M., Duzkale, H., Dagaonkar, N., Zhang, K., and Kalfa, T.A. (2016. )Clinical application of massively parallel sequencing in the diagnosis of hereditary hemolytic and dyserythropoietic anemias. Blood, 128 ,4746

Chonat, S., Risinger, M., Neha Dagaonkar, N., Maghathe, T., Rothman, J., Connor, J., Kalinyak, K., Begtrup, A.H., Zhang, K., and Kalfa, T.A. (2015. )The spectrum of alpha-spectrin associated hereditary spherocytosis. Blood, 126 ,941

Risinger, M.A., Glogowska, E., Begtrup, A.H., Dagaonkar, N., Chonat, S., Joiner, C.H., Quinn, C.T., Kalfa, T.A., and Gallagher, P.G. (2014. )The novel PIEZO1 mutation p.L2023V is causal for hereditary xerocytosis resulting in delayed channel inactivation and a dehydrated red blood cell phenotype. Blood, 124 ,abstract 741

Sokolic, R., Chonat, S., Risinger, M., Eckhaus, M., Garabedian, E., Calvo, K., Maric, I., Candotti, F., and Kalfa, T. (2014. )Cation leak in red blood cells of patients with Wiskott-Aldrich Syndrome leads to non-immunologic hemolysis. Blood, 124 ,abstract 1338

Bakeer, N., Luo, H.-Y., Grimley, M.S., Risinger, M., Manganaris, P.T., Begtrup, A.H., Chui, D.H.K., and Kalfa, T.A. (2014. )Hemoglobin Cincinnati: A novel beta globin gene mutation causing dominant beta thalassemia. .[Abstracy]Pediatr. Blood Cancer, 61 ,S15

Begtrup, A.H., Dagaonkar, N., Chonat, S., Giger, K.M., Husami, A., Kissell, D., Risinger, M., Joiner, C.H., Keddache, M., Zhang, K., and Kalfa, T.A. (2014. )Development of a comprehensive rapid Next-Generation sequencing assay for the diagnosis of inherited hemolytic anemia. .[Abstracy]Pediatr. Blood Cancer, (61 ),S59

Kalfa, T.A., Risinger M.A., Pushkaran, S., Dexheimer P., Husami, A., Kissell, D., Fletcher, D., Joiner, C.H., Keddache, M., and Zhang, K. (2012. )Next generation sequencing for diagnostic testing of erythrocyte cytoskeleton disorders. .[Abstracy]Blood, 120 ,abstract 976

Rinehart, J., Maksimova, Y.D., Tanis, J.E., Stone, K.L., Zhang, J., Risinger, M., Pan, W., Wu, D., Colangelo, C.M., Forbush, B., Joiner, C.H., Gulcicek, E.E., Gallagher, P.G., and Lifton, R.P. (2009. )Quantitative phosphoproteomics identifies sites in K-Cl cotransporters that regulate cell volume and neuronal excitation. http://www.asms.org., abstract 2246

Risinger, M., Rinehart, J., Crable, S., Ottlinger, A., Winkelmann, R., Pan, D., Huebner, C., Gallagher, P.G., and Clinton H. Joiner. (2008. )Structural and functional interactions of KCl Cotransport proteins KCC1 and KCC3 in sickle and normal erythrocyte membranes. Blood, 112 ,abstract

Pan, D., Kalfa, T.A., Wang, D., Risinger, M., Crable, S., Ciraolo, P., Franco, R.S., and Joiner, C.H. (2007. )Change in expressional profile of KCl cotransporter genes during human erythroid differentiation. Blood, 110 ,abstract 1709

Feldman, L., Rouleau, C., and Risinger, M.A. (1998. )Thrombopoietin has direct effects on the erythropoietin receptor in responsive erythroid progenitors. Blood, 92 ,580A

Risinger, M.A., Derick, L.H., and Cohen, C.M. (1994. )Melanocytes cultured from pallid mice are less melanized than C57 melanocytes and demonstrate differences in pallidin (band 4.2) localization. .[Abstracy]Mol. Biol. Cell, 5 ,366A

Risinger, M.A., Korsgren, C., and Cohen, C.M. (1992. )Expression and characterization of a nonmyristylated mutant human erythrocyte band 4.2. .[Abstracy]Mol. Biol. Cell, 3 ,211A

Risinger, M.A., Dotimas, E.M., and Cohen, C.M. (1991. )Both native and recombinant human erythrocyte band 4.2 produced in a eukaryotic expression system are myristylated. J. Cell Biol., 115 ,58A

Risinger, M.A., and Cohen, C.M. (1990. )Expression of human erythrocyte band 4.2 in a eukaryotic expression system. .[Abstracy]J. Cell Biol.,

Risinger, M.A., and Lin, S. (1989. )Localization of tensin in chicken embryo fibroblasts during cell-substrate contact formation and disruption. J. Cell Biol., 107 ,256A

Wilkins, J.A., Risinger, M.A., Coffey, E., and Lin, S. (1987. )Purification of a vinculin binding protein from smooth muscle. J. Cell Biol., 105 ,130A

Risinger, M.A., Wilkins, J.A., and Lin, S. (1987. )Comparison of tensin and vinculin localizations in muscle and nonmuscle cells. J. Cell Biol., 105 ,130A

Mullmann, T.J., Risinger, M.A., and Lin, S. (1987. )Immunoblot analyses with a monoclonal antibody against capactin, a protein that caps the barbed end of F-actin. .[Abstracy]J. Cell Biol., 105 ,195A

Lin, S., Risinger, M.A., Mullmann, T.J., Tseng, J., and Lin, D.C. (1987. )Effects of neoplastic transformation on the activity and cellular localization of actin regulator proteins in nonmuscle cells. In: Proceedings of International Meeting on the Biology of an Ubiquitous Protein: Actin. Monza, Italy.

Wilkins, J.A., Risinger, M.A., and Lin, S. (1986. )Immunologically related proteins at focal adhesions of nonmuscle and z-lines of muscle cells. J. Cell Biol., 103 ,111A

Risinger, M.A., Zachary, J.M., Kwock, L., and Lin, S. (1984. )Differences in cytoskeletal organization and endogenous cytochalasin-like activity between the low metastatic potential Dunning R-3327-H cell line and its high metastatic potential subline MAT LyLu. J. Cell Biol., 99 ,303A

Larsen, W.J., and Risinger, M.A. (1982. )The interaction of internalized cytoplasmic gap junction membrane with sterol-rich vesicles in SW-13 cells. Anat. Rec., 202 ,108A

Risinger, M.A., and Larsen, W.J. (1981. )Relationship of filipin-sterol complexes to tight junctions during their interiorization in a cultured ovarian adenocarcinoma. J. Cell Biol., 91 ,261A

Risinger, M.A., and Larsen, W.J. (1981. )Filament-membrane interactions in COLO 316 cells fixed prior to S-1 decoration. .[Abstracy]Anat. Rec., 199 ,212A

Risinger, M.A., and Larsen, W.J. (1980. )Endocytosis and degradation of tight junctions in the human ovarian adenocarcinoma cell line, COLO 316. .[Abstracy]Anat. Rec., 196 ,158A

Larsen, W.J., Tung, H.N., Risinger, M.A., and Murray, S.A. (1979. )Actin facilitated endocytosis and degradation of gap and tight junctions. .[Abstracy]J. Cell Biol., 83 ,87A

Peer Reviewed Publications

Niss, O., Chonat, S., Dagaonkar, N., Almansoori, M.O., Kerr, K., Rogers, Z.R., McGann, P.T., Quarmyne, M.-O., Risinger, M., Zhang, K., and Kalfa, T.A. (2016. )Genotype-phenotype correlations in hereditary elliptocytosis and hereditary pyropoikilocytosis. .Blood Cells Mol. Dis. , ,4-9

Konstantinidis, D.G., Giger, K.M., Risinger, M., Pushkaran, S., Zhou, P., Dexheimer, P., Yermeni, S., Andreassen P., Klingmuller, U., Palis, J., Zheng Y., and Kalfa, T.A. (2015. )Cytokinesis failure in RhoA-deficient mouse erythroblasts involves actomyosin and midbody dysregulation and triggers p53 activation. Blood, ,126 ,1473-1482

Pan, D., Kalfa, T.A., Wang, D., Risinger, M., Crable, S., Ottlinger, A., Chandra, S., Mount, D.B., Hubner, C.A., Franco, R.S., and Joiner, C.H. (2011. )K-Cl cotransporter gene expression during human and murine erythroid differentiation. .J. Biol. Chem. , ,286 ,30492-30503

Quarmyne, M.O., Risinger, M., Linkugel, A., Frazier, A., and Joiner, C. (2011. )Volume regulation and KCl cotransport in reticulocyte populations of sickle and normal red blood cells. .Blood Cells Mol. Dis., ,47 ,95-99

Hammill, A.M., Risinger, M.A., Joiner, C.H., Keddache, M., and Kalfa, T.A. (2011. )Compound heterozygosity for two novel mutations in the erythrocyte protein 4.2 gene causing spherocytosis in a Caucasian patient. .Br. J. Haematol., ,152 ,780-783

Rinehart, J., Maksimova, Y.D., Tanis, J.E., Stone, K.L., Hodson, C.A., Zhang, J., Risinger, M., Pan, W., Wu, D., Colangelo, C.M., Forbush, B., Joiner, C.H., Gulcicek, E.E., Gallagher, P.G., and Lifton, R.P. (2009. )Sites of regulated phosphorylation that control K-Cl cotransporter activity. Cell, ,138 ,525-536

Joiner, C.H., Rettig, R.K., Jiang, M., Risinger, M., and Franco, R.S. (2007. )Urea stimulation of KCl cotransport induces abnornmal volume reduction in sickle reticulocytes. .Blood, ,109 ,1728-1735

Goss, K.H., Risinger, M.A., Kordich, J.J., Sanz, M.M., Straughen, J.E., Sovek, L.E., Capobianco, A.J., German, J., Boivin, G.P., and Groden, J. (2002. )Enhanced tumor formation in mice heterozygous for Blm mutation. .Science, ,297 ,2051-2053

Oakley, G.G., Loberg, L.I., Yao, J., Risinger, M.A., Yunker, R.L., Zernik-Kobak, M., Khanna, K.K., Lavin, M.F., Carty, M.P., and Dixon, K. (2001. )UV-induced hyperphosphorylation of replication protein A depends on DNA replication and expression of ATM protein. Mol. Biol. Cell, ,12 ,1199-1213

Sytkowski, A.J., Lunn, E.D., Risinger, M.A., and Davis, K.L. (1999. )An erythropoietin fusion protein comprised of identical repeating domains exhibits enhanced biological properties. J. Biol. Chem., ,274 ,24773-24778

Risinger, M.A., Korsgren, C., and Cohen, C.M. (1996. )Role of N-myristylation in targeting of band 4.2 (pallidin) in non-erythroid cells. Exp. Cell Res. , ,229 ,421-431

Hayette, S., Morle, L., Bozon, M., Ghanem, E., Risinger, M., Korsgren, C., Tanner, M.J.A., Fattoum, S., Cohen, C.M., and Delaunay, J. (1995. )A point mutation in the protein 4.2 gene (allele 4.2 Tozeur) associated with hereditary haemolytic anaemia. .Br. J. Haematol., ,89 ,762-770

Risinger, M.A., Dotimas, E.M., and Cohen, C.M. (1992. )Human erythrocyte band 4.2, a high copy number membrane protein, is N-myristylated. J. Biol. Chem., ,267 ,5680-5685

Zachary, J.M., Cleveland, G., Kwock, L., Lawrence, T., Weissman, R.M., Nabell, L., Fried, F.A., Staab, E.V., Risinger, M.A., and Lin, S. (1986. )Actin filament organization of the Dunning R-3327 rat prostatic adenocarcinoma system: Correlation with metastatic potential. .Cancer Res. , ,46 ,926-932

Wilkins, J.A., Risinger, M.A., and Lin, S. (1986. )Studies on proteins that co-purify with smooth muscle vinculin. Identification of immunologically related species in focal adhesions of nonmuscle and z-lines of muscle cells. .J. Cell Biol., ,103 ,1483-1494

Risinger, M.A., and Larsen, W.J. (1982. )Interaction of filipin with junctional membrane at different stages of the junction's life history. .Tissue and Cell , ,15 ,1-15

Risinger, M.A., and Larsen, W.J. (1981. )Endocytosis of cell-cell junctions and spontaneous cell disaggregation in a cultured human ovarian adenocarcinoma (COLO 316). .Tissue and Cell, ,13 ,413-430

Book Chapter

Risinger, M.A., and Groden, J. (2004 )recQ DNA helicase family in genetic stability. Encyclopedia of Biological Chemistry, vol 3 .(pp. 660-664).Oxford, UK,Elsevier Science

Korsgren, C., Risinger, M.A. and Cohen, C.M. (2000 )Human erythrocyte protein band 4.2. Human Protein Data .Weinheim, Germany,VCH

Lin, S., Risinger, M.A., and Butler, J.A. (1988 )A model for protein-protein interactions involved in the linkage of the actin cytoskeleton to transmembrane receptors for extracellular matrix proteins. Structure, Interactions and Assembly of Cytoskeletal and Extracellular Proteins .Springer Series in Biophysics

Lin, S., Risinger, M.A., Wilkins, J.A., Mullmann, T.J., Butler, J.A., Wachsstock, D.H., and Lin, D.C. (1987 )Proteins involved in the assembly and membrane-attachment of actin filaments in muscle and nonmuscle cells.

Lin, S., Lees, A., Risinger, M.A., Cribbs, D.H., Wilkins, J.A., and Lin, D. (1986 )Effects of several actin binding proteins on nucleation, elongation and depolymerization of actin filaments in vitro. Cell Motility: Mechanisms and Regulation .Tokyo, Japan,Univ. of Tokyo Press

Larsen, W.J., and Risinger, M.A. (1985 )The dynamic life histories of intercellular membrane junctions. Modern Cell Biology, vol 4 .Alan R. Liss, Inc.

Additional Publications

Presentations

Poster Presentations

Risinger, M., Dagaonkar, N., Christakopoulos, G., Liu, J., Seu, K.G., Konstantinidis, D., Quinn, C., Chonat, S., Joiner, C.H., Zhang, K., Kalfa, T.A. (12-2016. )Cellular hydration and oxidation as phenotype modifiers in sickle cell anemia. .American Society of Hematology, San Diego, CA. .Level:National

Lesmana, H., Christakopoulos, G.E., Seu, K.G., Risinger, M., Duzkale, H., Dagaonkar, N., Zhang, K., and Kalfa, T.A. (12-2016. )Clinical application of massively parallel sequencing in the diagnosis of hereditary hemolytic and dyserythropoietic anemias. .American Society of Hematology, San Diego, CA. .Level:National

Gupta, S., Baskin, J., Kalfa, T., Risinger, M., and Zhang, K. (05-2016. )Hereditary hemolytic anemia due to red cell monovalent cation leak in a patient with a novel band 3 mutation. American Society of Pediatric Hematology/Oncology, Minneapolis, MN. .Level:National

Chonat, S., Risinger, M., Neha Dagaonkar, N., Maghathe, T., Rothman, J., Connor, J., Kalinyak, K., Begtrup, A.H., Zhang, K., and Kalfa, T.A. (12-2015. )The spectrum of alpha-spectrin associated hereditary spherocytosis. American Society of Hematology, Orlando, FL. .Level:National

Sokolic, R., Chonat, S., Risinger, M., Eckhaus, M., Garabedian, E., Calvo, K., Maric, I., Candotti, F., and Kalfa, T. (12-2014. )Cation leak in red blood cells of patients with Wiskott-Aldrich Syndrome leads to non-immunologic hemolysis. American Society of Hematology, San Francisco, CA. .Level:National

Bakeer, N., Luo, H.-Y., Grimley, M.S., Risinger, M., Manganaris, P.T., Begtrup, A.H., Chui, D.H.K., and Kalfa, T.A. (05-2014. )Hemoglobin Cincinnati: A novel beta globin gene mutation causing dominant beta thalassemia. .American Society of Pediatric Hematology/Oncology, Chicago, IL. .Level:National

Begtrup, A.H., Dagaonkar, N., Chonat, S., Giger, K.M., Husami, A., Kissell, D., Risinger, M., Joiner, C.H., Keddache, M., Zhang, K., and Kalfa, T.A. (05-2014. )Development of a comprehensive rapid Next-Generation sequencing assay for the diagnosis of inherited hemolytic anemia. .American Society of Pediatric Hematology/Oncology, Chicago, IL. .Level:National

Begtrup, A.H., Dagaonkar, N., Chonat, S., Giger, K.M., Husami, A., Kissell, D., Risinger, M., Joiner, C.H., Keddache, M., Zhang, K., and Kalfa, T.A. (12-2013. )Development of a comprehensive rapid Next-Generation sequencing assay for the diagnosis of inherited hemolytic anemia. .American Society of Hematology, New Orleans, LA. .Level:National

Risinger, M.A., Glogowska, E., Begtrup, A.H., Dagaonkar, N., Chonat, S., Joiner, C.H., Quinn, C.T., Kalfa, T.A., and Gallagher, P.G. (05-2015. )The novel PIEZO1 mutation p.L2023V is causal for hereditary xerocytosis resulting in delayed channel inactivation and a dehydrated red blood cell phenotype. .University of Cincinnati Research Week, Cincinnati, OH. .Level:Local

Kalfa, T.A., Risinger M.A., Pushkaran, S., Dexheimer P., Husami, A., Kissell, D., Fletcher, D., Joiner, C.H., Keddache, M., and Zhang, K. (10-2013. )Next generation sequencing for diagnostic testing of erythrocyte cytoskeleton disorders. .University of Cincinnati Research Week, Cincinnati, OH. .Level:Local

Lecture

Risinger, M., Brehm, B., and Osterman, R. (03-2015. )Using Biomarkers in Nursing Research .Cincinnati, OH. UC. Level:College

Risinger, M. (11-2016. )Cellular Hydration and Oxidation as Phenotype Modifiers .Cincinnati, OH. UC. Level:University

Risinger, M. (11-2016. )Cellular Hydration and Oxidation as Phenotype Modifiers .Cincinnati, OH. UC. Level:University

Paper Presentations

Risinger, M.A., Glogowska, E., Begtrup, A.H., Dagaonkar, N., Chonat, S., Joiner, C.H., Quinn, C.T., Kalfa, T.A., and Gallagher, P.G. (12-2014. )The novel PIEZO1 mutation p.L2023V is causal for hereditary xerocytosis resulting in delayed channel inactivation and a dehydrated red blood cell phenotype. .San Francisco, CA. Professional Meeting. Level:National

Service

(Faculty Search Committee )Committee Member Type:Departmental Service 2014 -To Present

(PhD Program Preliminary Examination Committee )Committee Member Type:Departmental Service 2014 -To Present

(HESI test proctor )Type:Departmental Service 2015 -To Present

(Organized task force to evaluate "omics" and informatics courses available to CoN PhD students )Type:Departmental Service 2016

(Attended Research Ethics Workshop sponsored by the University of California San Diego Research Ethics Program in cooperation with the UC Office of Research as the representative of the CoN )Type:Departmental Service 05-2015

(Assisted with CoN recruiting activities at the Annual MNRS Meeting in Indianapolis )Type:Departmental Service 04-2015

(Served as CoN representative on Search Committee to select a Research and Education Librarian to provide services to the UC Academic Health Center )Type:University/College Service 2016

(University Research Council Life Science Review Committee )Reviewer Type:University/College Service 2015 -2016

(University Research Council Behavioral and Social Sciences Review Committee )Reviewer Type:University/College Service 2014 -2015

Positions and Work Experience

10-2013 - Assistant Professor, Research Track, University of Cincinnati, College of Nursing, Cincinnati, OH

03-2006 -09-2012 Research Associate, Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, OH

09-2000 -09-2005 Research Associate, Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati, Cincinnati, OH

11-1999 -06-2000 Independent Contractor, Department of Environmental Health, University of Cincinnati, Cincinnati, OH

02-1998 -08-1999 Associate Scientist, Beth Israel Deaconess Medical Center, Harvard University, Boston, MA

05-1989 -02-1998 Assistant Professor, Department of Medicine, Tufts University, Boston, MA

02-1989 -02-1998 Assistant Investigator, Department of Biomedical Research, St. Elizabeth's Medical Center, Boston, MA

07-1987 -01-1989 Research Associate, Department of Biophysics, Johns Hopkins University, Baltimore, MD

07-1983 -06-1987 Postdoctoral Fellow, Department of Biophysics, Johns Hopkins University, Baltimore, MD

08-1979 -08-1981 Teaching Assistant, Department of Anatomy and Cell Biology, University of Iowa, Iowa City, IA

01-1976 -05-1978 Instructor, Department of Nursing, Mt. Mercy University, Cedar Rapids, IA

05-1972 -08-1974 Staff Nurse, Mercy Hospital, Davenport, IA

Post Graduate Training and Education

1984-1987 NIH National Research Service Award, Cytochalasin-like Activity in Transformed Fibroblasts, Johns Hopkins University, ,Baltimore, MD

Research and Practice Interests

Research interests include:
• Genomic Polymorphisms in Sickle Cell Disease
• Genes that Affect Hydration and Reactive Oxygen Species (ROS) Production in
        Erythrocytes 
• Erythrocyte Cytoskeleton Disorders
• Erythrocyte Volume Regulation
 

Professional Affiliation

2017 -To Present: American Nurses Association

2017 -To Present: Ohio Nurses Association

2015 -To Present: Midwest Nursing Research Society

2014 -To Present: American Society of University Professors

2014 -To Present: Ohio Valley Sickle Cell Network

2013 -To Present: Board of Editors in the Life Sciences

Courses Taught

Level:Graduate

Faculty Development Activities

04-18-2017 Write Winning Grant Proposals sponsored by UC Office of Research Cincinnati, OH Type:Seminar

07-25-2016 -07-29-2016 NINR Precision Health: From "Omics" to Data Science Boot Camp Foundation for Advanced Education in the Sciences at the NIH Washington, DC

04-16-2015 -04-19-2015 Innovations for Person Centered Care Midwest Nursing Research Society 2015 Annual Research Conference Indianapolis, IN Type:Conference Attendance

12-06-2014 -12-09-2014 American Society of Hematology Annual Meeting 2014 San Francisco, CA Type:Conference Attendance

12-03-2016 -12-06-2016 American Society of Hematology Annual Meeting 2016 San Diego, CA Type:Conference Attendance

Contact Information

229 Procter Hall
Phone: 513-558-5258
Fax: 513-558-2142