UC Flow Cytometry Core

William Miller PhD

The Flow Core supports a Beckman CytoFLEX S with 9 detectors and 4 lasers in CARE 2874 and a FACSCalibur 4 color flow cytometer system in CARE 2876.  Access is available 24 hrs a day and 7 days per week once investigators are registered in Stratocore and approved by the core director.

For access to the instruments and the online calendar, please contact Dr. Miller.

Services2
  • FACSCalibur System access (2 Laser, 4 color)

    The FACSCalibur is configured as follows:

    • FL1 Channel - Blue Laser (488nm) and 515-545nm Emission Filter (FITC, GFP, etc.)
    • FL2 Channel - Blue Laser (488nm) and 564-601nm Emission Filter (PE, PI, RFP, etc.)
    • FL3 Channel - Blue Laser (488nm) and 670LP Emission Filter (7-AAD, PerCP, etc)
    • FL4 Channel - Red Laser (635nm) and 653-669nm Emission Filter (APC, Cy5, etc.)
    It is running the BS CellQuest Pro Software.

    Cost:

    $20 per half hour

    Request Information
  • CytoFLEX S System Access (4 laser, 9 color)

    Here are the channels currently configured (in bold) the ones in italics can be utilized by repositioning the filters, which is relatively easy to do.
     
    CytoFLEX (4 laser, 9 color)
     
    Violet Laser (405nm)                               Emmision Filter
    PB450 (PacBlue, eFluor450)                           450/45
    KO525 (Krome Orange, Zombie Aqua)           525/40
    BV605                                                              610/20
    BV650                                                              660/20
    BV786                                                              780/60
     
    Blue Laser (488nm)
    FITC (GFP, Alexa488, Venus)                           525/40
    PerCP (PC5, PC5.5)                                         690/50
     
    Yellow/Green Laser (561nm)
    PE (PI, Sytox Orange, DsRed, TdTomato)       585/42
    ECD (mCherry, Texas Red)                              610/20
    PC7                                                                   780/60
    PC5                                                                   690/50
     
    Red Laser (638nm)
    APC (AlexaFluor 647, eFluor660)                    660/10
    APC-A750 (APC-Cy7, APC eFluor780             780/60
    APC-A700 (AlexaFluor 700)             &n

    Cost:

    $20 per half hour

    Request Information
Equipment2
  • FACSCalibur Four Color Flow Cytometer

    The FACSCalibur is configured as follows:

    • FL1 Channel - Blue Laser (488nm) and 515-545nm Emission Filter (FITC, GFP, etc.)
    • FL2 Channel - Blue Laser (488nm) and 564-601nm Emission Filter (PE, PI, RFP, etc.)
    • FL3 Channel - Blue Laser (488nm) and 670LP Emission Filter (7-AAD, PerCP, etc)
    • FL4 Channel - Red Laser (635nm) and 653-669nm Emission Filter (APC, Cy5, etc.)
    It is running the BS CellQuest Pro Software.
     
     

    Request Information
  • Beckman CytoFLEX S V2-B2-Y3-R2 Flow Cytometer (9 Detectors, 4 Lasers)

    Here are the channels currently configured (in bold) the ones in italics can be utilized by repositioning the filters, which is relatively easy to do.
     
    CytoFLEX (4 laser, 9 color)
     
    Violet Laser (405nm)                               Emmision Filter
    PB450 (PacBlue, eFluor450)                           450/45
    KO525 (Krome Orange, Zombie Aqua)           525/40
    BV605                                                              610/20
    BV650                                                              660/20
    BV786                                                              780/60
     
    Blue Laser (488nm)
    FITC (GFP, Alexa488, Venus)                           525/40
    PerCP (PC5, PC5.5)                                         690/50
     
    Yellow/Green Laser (561nm)
    PE (PI, Sytox Orange, DsRed, TdTomato)       585/42
    ECD (mCherry, Texas Red)                              610/20
    PC7                                                                   780/60
    PC5                                                                   690/50
     
    Red Laser (638nm)
    APC (AlexaFluor 647, eFluor660)                    660/10
    APC-A750 (APC-Cy7, APC eFluor780             780/60
    APC-A700 (AlexaFluor 700)      

    Request Information