Advanced Cell Analysis Service Center (ACASC)
To assist the researcher in generating high-resolution, high quality, microscopy-based data for publications and presentation at professional venues. A range of services is available for both experienced and inexperienced users. Experienced users may use the Center's instruments after orientation by a staff member. Inexperienced users may choose to receive training in the use of the instruments, technical support in microscopy and image analysis, consultation in experimental design, or have us perform the microscopy for them as a service.
To book equipment or access services for the ACASC, please login or create an account through Stratocore at https://ppms.us/uc/start/
Incucyte Zoom (extended live cell microscopy)Cost:Request Information
Image Analysis and Output (ImageJ, deconvolution, 3-D, Photoshop, printers)Cost:Request Information
Digital Macroscopic Imaging (transgenic animals, organs, 2-D gels)Cost:Request Information
Confocal Microscopy (Zeiss LSM 710)Cost:Request Information
Low Resolution Widefield Fluorescent and Brightfield MicroscopyCost:Request Information
High resolution fluorescence and brightfield microscopyCost:Request Information
Center Staff timeCost:Request Information
Nikon SIMe superresolution microscopyCost:Request Information
Confocal Microscopy (Zeiss LSM 710, inverted microscope)
A Zeiss Axio Observer Z1 inverted microscope is connected to a Zeiss LSM710 confocal. The available laser lines are 405, 458, 488, 514, 561 and 633nm. With the availability of a near UV laser this confocal can also visualize DAPI. In addition to the confocal images a DIC image can be acquired. Stage and objective heaters are available to aid live cell imaging.Request Information
The most common reason to use a confocal is to obtain optical sections that have much less out-of-focus blur than images from widefield instruments. In addition, one can acquire a 3-D data set for volume determination or 3-D reconstruction.
Multi-tracking provides considerable improvement in the separation of similar dyes over that of a widefield microscope. Therefore, even if you do not need the optical sectioning ability of the confocal microscope, you may want to use it instead of a widefield microscope in order to ensure separation of dye pairs like FITC and rhodamine. Another reason to chose a confocal over a widefield microscope is to have precision in the overlay of images that are taken with different filters.
Widefield Light Microscopy (multiple stations, upright & inverted platforms)
A Zeiss Axioplan Imaging 2e infinity-corrected upright scope with DIC and epifluorescence. Uses either a Color Zeiss Axiocam (for brightfield imaging) or a B&W Zeiss Axiocam for fluorescence. Filter cubes are available for DAPI, FITC, rhodamine, Texas Red and Cy5-like dyes.Request Information
Axiovert 100 TV inverted microscope
This inverted scope is equipped for phase, brightfield, and fluorescence. The filter cubes are suitable for fluoresceine (GFP), rhodamine, and DAPI like dyes. The available objectives are 1.25x, 5x, 10x and a 32x long working distance objective. Additional objectives are available upon request. The filters and optics on this scope are not as advanced as the orcaerzeiss but it is useful for e.g. checking on transfection efficiency. A Zeiss Axiocam MRm is connected connected to the microscope and provides as resolution of up to 1388 x 1040 pixels. The camera is suitable for fluorescence imaging and provides only B&W images.Request Information
An Olympus SZX12 stereo microscope serves for low magnification microscopy needs. It is equipped with a Q-imaging color camera and uses Q-Capture software to acquire images.Request Information
IncuCyte ZOOM® consists of a microscope inside a standard cell incubator for environmental stability, and a networked external controller hard drive that collects and processes image data. The instrument has three microscope objectives (4x, 10x, 20x) that can be interchanged by the facility manager upon request.Request Information
It houses up to six microtiter plates which can be run simultaneously. Available imaging modes are phase contrast, red and green fluorescent. Imaging can be for extended periods of time.
Hardware and software tools for a scratch wound migration assay in 96-well plates is available.
Software can be remotely accessed by any networked computer (IP number 10.170.3.126). Ask Birgit for a download location of the program.
A Nikon SIMe (structured illumination microscopy) superresolution microscope is available on a motorized inverted Nikon Eclipse Ti stand. Available laser lines are 488nm, 561nm and 640nm for acquiring 3D-SIM images. A DAPI channel can be added as a fluorescent widefield channel. The system provides lateral resolution of up to 115nm and axial resolution of up to 269nm.Request Information
The system is equipped with a Hamamatsu Orca Flash 4.0 camera with high sensitivity and low readout noise.
Publications which relied on the equipment:
Chen et al. (2019). Nanoscale monitoring of mitochondria and lysosome interactions for drug screening and discovery. Nano Research 12(5):1009–1015.
Khamo et al. (2019). Optogenetic Delineation of Receptor Tyrosine Kinase Subcircuits in PC12 Cell Differentiation. Cell Chemical Biology 26(3): 400-410.e3
Chen et al. (2018) Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore. Small 14 (41): 1802166