Live Microscopy Core

Chet Closson, Manager

The Live Microscopy facility is designed to help investigators perform high resolution imaging with either living or fixed specimens. The facility has two advanced Zeiss confocal laser scanning microscopes for use, including one equipped for multiphoton imaging, as well as a Leica DMi8 widefield microscope system, and various stereo and dissection microscopes. Additional equipment available for use are a Laser Capture Microdissection instrument, multimode plate reader, Real-Time-PCR systems, infrared imager, and cryostat.

Location & Hours:
The core is located in Medical Sciences Building Room 3155. It is open 24/7 for approved trained users, or from 9 am to 5 pm, Monday through Friday for technical assistance.

Services12
  • Confocal/Multiphoton Microscopes: Prime Hours Usage (per hour, Mon-Fri, 9am-5pm)

    Cost:

    $35

    Request Information
  • Leica Cryostat (unlimited access per year)

    Cost:

    $200

    Request Information
  • Leica Stereo Microscope (unlimited access per year)

    Cost:

    $200

    Request Information
  • PerkinElmer EnVision Multi Mode Plate Reader (unlimited access per year)

    Cost:

    $200

    Request Information
  • Confocal/Multiphoton Microscopes: Off Hours Usage (per hour, all times not covered by Prime Hours)

    Cost:

    $25

    Request Information
  • Widefield Microscopes: Prime Hours Usage (per hour, Mon-Fri, 9am-5pm)

    Cost:

    $10

    Request Information
  • Widefield Microscopes: Off Hours Usage (per hour, all times not covered by Prime Hours)

    Cost:

    $5

    Request Information
  • Technician Assistance: Training or Collecting Images (per hour)

    Cost:

    $70

    Request Information
  • ArcturusXT Laser Capture MicroDissection (per hour)

    Cost:

    $40

    Request Information
  • QuantStudio 5 RT-PCR (per plate)

    Cost:

    $10

    Request Information
  • StepOnePlus RT-PCR (per plate)

    Cost:

    $10

    Request Information
  • Li-Cor Odyssey Infrared Imager (unlimited access per year)

    Cost:

    $200

    Request Information
Equipment3
  • LSM 710 LIVE Duo Confocal Microscope

    Equipped with two separate scanning units, the fast-speed line (LIVE) scanner (up to 120 frames/sec at 512x512) and the LSM710 high-resolution point scanner, integrated into the same microscope. Point Scanner: 6 laser lines (405, 458, 488, 514, 560, 633nm) and 4 detectors (3 spectral confocal fluorescence plus 1 transmitted light detector). LIVE fast line scanner: 3 laser lines (405, 488, 560nm) and 2 confocal fluorescence detectors. The Duo system allows the two scanners to be used in combination for complex, high-speed bleaching or photomanipulation experiments. Temperature-controlled environmental chamber encasing entire microscope.

    Request Information
  • LSM 510 NLO Two-Photon Microscope

    4 lasers (Ti-Sa [700-990 nm; wavelength selection under computer control], Ar [458,477,488,514 nm], Green HeNe [543 nm] and Red HeNe [633 nm]), 6 detectors [3 confocal fluorescence/reflectance, 1 Nomarski transmitted, 2 non-descanned high sensitivity two-photon detectors]). Inverted microscope stage enclosed in light-tight, temperature-controlled environmental chamber. Useful for deep tissue imaging (better than standard confocal) while retaining subcellular resolution in living tissue. The two-photon absorption also allows fluorophore uncaging, cell damage/ablation, spectral shifting, and other photo-catalytic events to be performed with sub-micron resolution in three dimensions.

    Request Information
  • DMi8 Widefield Fluorescence Microscope

    Widefield microscope system equipped with a stage-top incubation chamber for live time-lapse imaging experiments in brightfield and fluorescence. Color camera for histology and phase contrast imaging, and high-sensitivity Hamamatsu ORCA-ER cooled CCD camera for fluorescence imaging. Sola LED light source and 4 fluorescence filters (DAPI, GFP, Texas Red, CY5). Motorized stage enables image stitching of large regions at high magnification.

    Request Information