UC Proteomics Laboratory (UCPL)

Ken Greis, PhD., Director

The UC Proteomics Laboratory (UCPL) is committed to providing collaborative expertise & services in proteomics & biological mass spectrometry both as fee-for-service and as grant supported partnerships.

Routine Analyses

  • Comparative protein profiling by 2D gel
  • Protein identification by Mass Spectrometry
  • Characterization of Protein Complexes
  • Confirming and Mapping Protein Modification sites

Advanced Services/Collaborative Projects
  • Comparative protein profiling by MS (SILAC, iTRAQ)
  • Label Free Quantitation using Data Independent Aquisition (DIA)
  • Global Profiling of Protein Modifications (e.g. phospho, ubiquitin)

Technology development research
  • Additional Global PTM profiling
  • Complex MRM quantitation of targeted proteins
  • Enzyme assays and inhibitor screening

Location & Hours:
The UCPL is located in the Vontz Center for Molecular Studies 1208-1216. Staff are typically available M-F, 9A-6P to accept samples, but please call 513 558-4057 to confirm.  First time customers should include a discussion with the director or associate director prior to preparing samples.

Services15
  • Mini 2D gel protein separation per gel

    Cost:

    115.00

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  • Mini 1D gel protein separation per gel

    Cost:

    85.00

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  • iTRAQ sample preparation and labeling per 4-plex sample (<100ug)

    Cost:

    355.00

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  • Preparative High pH RP separation of peptides with fraction collection per sample

    Cost:

    90.00

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  • Data Processing and Report Generation per hour

    Cost:

    40.00

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  • Research Staff hourly rate

    Cost:

    40.00

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  • MALDI-TOF/TOF and protein ID per sample

    Cost:

    65.00

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  • nanoLC-MS/MS for separation & fragmentation of peptides per sample

    Cost:

    160.00

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  • MALDI-TOF of intact protein per sample

    Cost:

    60.00

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  • Sample preparation by buffer exchange per sample

    Cost:

    20.00

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  • Serum immunodepletion by IgY spin columns per sample

    Cost:

    30.00

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  • Protein Concentration by Pierce 660 assay per sample

    Cost:

    15.00

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  • Large format 2D gel protein separation per sample

    Cost:

    165.00

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  • Gel Stain (Silver Coomassie) per gel

    Cost:

    30.00

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  • Protease digestion and peptide recovery per sample

    Cost:

    35.00

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Equipment4
  • Sciex 5600+ Quadrapole-Tof Mass Spectrometer w/Eksigent nanoLC-Ultra 2D System

    This instrument offers the hybrid capabilities for high resolution and high sensitivity profiling, characterization of compounds in complex mixtures, and also high selectivity and sensitivity for quantitation of compounds in complex mixtures by monitoring fragmentations transitions either in selected reaction monitoring (SRM) or multiple reaction monitoring (MRM) modes.  With attomole sensitivity, this system is optimized for both quantitative proteomics profiling using either label free or isotope tagging methods (e.g. SILAC, iTRAQ) and quantitation of specific proteins (as peptide surrogates) from complex mixtures.

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  • Sciex 4800 MALDI-TOF/TOF MS


    The 4800 instrument is the primary instrument in the proteomics laboratory used for core services that require protein identification from single bands or spot from gels.  The attomole sensitivity and high repetition laser for fast data collection make it the ideal option for protein identification including protein complexes captured by affinity methods.  This instrument is also the primary system used for the rapid readout of enzyme assays and inhibitor screening studies.

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  • Beckman Coulter NX Liquid Handling Workstation

    Beckman Coulter NX Liquid Handling Workstation

    This system is use for samples preparation of enzyme reactions for MS-bases screening activities.

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  • Computational Systems and Software for Data Analysis

    Computational systems and software for data analysis including:
     

    1. 10 terrabyte general file server for automatic backup and archival of all proteomics data and reports.
    2. 8-processor MASCOT server for high throughput identification and quantification of proteins.
    3. 12-processor system running AB Sciex ProteinPilot software for relative quantitation of label-free or isotope tagged peptides

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