UC Proteomics Laboratory (UCPL)
Ken Greis, PhD., Director
To access services from the UCPL, please login or create an account in Stratocore at https://ppms.us/uc/start/
Standard Service
- Protein identification by Mass Spectrometry
- Characterization of Protein Complexes
- Confirming and Mapping Protein Modification sites
- Comparative protein profiling:
- Comparative MS-based profiling using Label-Free Quantitation (LFQ) and Isotope Tagging methods (SILAC, TMTs)
- Protein proximity analyses for BioID, APEX and related methods
Advanced Services/Collaborative Projects
- Global Profiling of Protein Modifications (e.g. phospho, ubiquitin)
- Complex MRM quantitation of targeted proteins
- Enzyme assays and inhibitor screening by MS
Location & Hours:
The UCPL is located in the Vontz Center for Molecular Studies 1208-1216. Staff are typically available M-F, 9A-6P to accept samples, but please call 513 558-4057 to confirm. First time customers should include a discussion with the director or associate director prior to preparing samples.
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Protein Concentration by Pierce 660 assay per sample
Cost:Request Information40
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Orbitrap FUSION-LUMOS LC-MS instrument time per hr
Cost:Request Information50
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Data Processing and Report Generation per hour
Cost:Request Information50
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Research Staff hourly rate
Cost:Request Information50
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Materials and reagents pass through cost
Cost:Request InformationInquire
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Protease digestion and peptide recovery per sample
Cost:Request Information40
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Mini 1D gel protein separation per gel
Cost:Request Information100
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Mini 2D gel protein separation per gel
Cost:Request Information150
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Gel Stain (Silver, Coomassie) per gel
Cost:Request Information70
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Sample preparation by buffer exchange per sample
Cost:Request Information30
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Preparative High pH RP separation of peptides with fraction collection per sample
Cost:Request Information200
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Orbitrap ECLIPSE LC-MS instrument time per hr
Cost:Request Information50
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Computational Systems and Software for Data Analysis include:
- 20 terrabyte general file server for automatic backup and archival of all proteomics data and reports.
- 12-processor system running Sciex ProteinPilot software for relative quantitation of label-free or isotope tagged peptides
- 16-processor system running the Thermo Proteome Discoverer suite of data analysis software.
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Thermo Oribitrap Eclipse Tribrid mass spectrometer
This newest system acquired via an NIH S10 instrumentation grant in October of 2019 is configured with a Dionex Ultimate 3000 RSLCnano LC and a FAIMS Pro High Field Asymmetric Waveform Ion Mobility Spectrometry Interface to allow for maximum separation and detection of peptides in complex mixtures. This system is ideally suited for high throughput and high sensitivity comparative quantitative studies using Tandem Mass Tag (TMT) reagents or label-free quantitation methods. With up to 5X better sensitivity compared to the Quadrupole-Tof systems in the lab, this system is used for global profiling of protein modifications (e.g. phosphorylation, ubiquitination) and for multiplex profiling studies of large number of samples.
Request Information -
Thermo Oribitrap Fusion Lumos Tribrid mass spectrometer
This high resolution mass spectrometer coupled with a Thermo Vanquish UHPLC system. This system is primarily dedicated to the development of new metabolomics profiling capabilities include steady state and metabolic flux analysis, as well as lipids, nucleosides, fatty acids and other metabolites. With its high resolution accurate mass (HRAM), it is ideally suited for structural elucidate of small molecule via intact mass and fragmentation spectra for metabolomics needs. With its rapid duty cycle and attomole sensitivity, it is dedicated for use to evaluate and quantify very complex sample mixtures for Metabolomics capabilities
Request Information