Genomics, Epigenomics and Sequencing Core (GESC)

Xiang Zhang, PhD; Core Director & Assoc. Prof.

The Genomics, Epigenomics and Sequencing Core (GESC), originally named as Sequencing and Microarray Laboratory, was established in 1999. GESC is a fee-for-service, one-stop facility that provides genomics and epigenomics related service and collaboration to researchers at the University of Cincinnati, Cincinnati Children's Hospital and other institutions.
In 2012, GESC was transformed into the next generation sequencing-based core facility. Since then, it has been expanding its services and seamlessly integrated with the bioinformatics team for handling massive amounts of data generated by these services. Equipped with Illumina NestSeq 550 sequencer and automated liquid handling system, GESC provides a wide variety of high-quality NGS services and continuously developing new methodologies to meet researchers’ requirements. In addition, it offers Illumina BeadChip assay and a series of other supportive services. The Core Director Xiang Zhang routinely provides consultation to researchers from sample preparation to experiment design.
GESC is a small but highly efficient team. Nearly 100% of its expenses is paid from its service revenue. The Core’s financial status is extraordinary healthy and stable.

Services10
  • RNA/DNA extraction (depending on sample type and number; human blood and clinical samples: $40)

    Service rate is depdending on sample type and number. $40/sample for human blood and clinical samples.

    Cost:

    $25 or $40

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  • Qubit assay (accurately measure dsDNA concentration)

    Accurately measure dsDNA concentration.

    Cost:

    $3.5

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  • DNA/RNA Bioanalyzer QC (measure sample size distribution and integrity)

    Cost:

    $25

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  • miRNA-seq (standard service; SR 1x85 bp, ~3M reads)

    Cost:

    $240

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  • PolyA RNA-seq (standard service; SR1x85 bp, >20M pass filter reads)

    Cost:

    $270

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  • Other types of sequencing (varies with experiment approach, kit/reagent, sequencing setting, etc.)

    Cost:

    Please inquire

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  • MethylationEPIC/oxBS-methylationEPIC (extra charge for repeated assay (sample #: 8X) if the low data quality is not due to our mistake)

    Cost:

    Please inquire

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  • Other types of services (supportive DNA/RNA experiments)

    Cost:

    Please inquire

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  • Note 1: The rates are approved by UC Recharging Meeting. Due to the price change of kits, reagents, consumables, etc., they are subject to change without prior notice.

    Cost:

    Please email xiang.zhang@uc.edu

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  • Note 2: Collaboration and/or authorship maybe required if the Core has significant intellectual contribution.

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Equipment1
  • GES Core major equipment

    NextSeq 550 sequencer (Illumina, San Diego, CA)
    SMARTer Apollo automatic liquid handling system (Takara Bio USA, Mountain View, CA)
    iScan system for Infinium beadchip Assay (Illumina, San Diego, CA)
    FastPrep-24 5G homogenizer (MP Bio Solon, OH)
    Bioanalyzer for DNA/RNA QC analysis (Agilent, Santa Clara, CA)
    CentriVap micro IR Vacuum Concentrator (Labconco, Kansas City, MO)
    Qubit 3.0 fluorometer (Thermo Fisher, Waltham, MA)
    Covaris S2 Focused-ultrasonicators for precise DNA/chromatin shearing (Covaris, Woburn, MA)
    ABI QuantStudio 5 Real-Time PCR System (Thermo Fisher, Waltham, MA)
    Veriti thermal cycles (Thermo Fisher, Waltham, MA)
    Enduro GDS Touch Gel Documentation System agarose (Labnet Edison, NJ)
    Desktop and floor centrifuges
    Incubators, heaters and shakers
    Revco high-performance ultra-low freezers, freezers and refrigerators (Thermo Fisher, Waltham, MA)
    Smart- Uninterruptible Power Supplies (Schneider Rueil-Malmaison, France)
    Branson 5800 Series Ultrasonic Cleaner (Danbury, CT)
    Servers, high performance computers, massive data storage devices

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