Genomics, Epigenomics and Sequencing Core (GESC)
Xiang Zhang, PhD; Core Director & Assoc. Prof.
The Genomics, Epigenomics and Sequencing Core (GES Core), originally named as Microarray Laboratory, was established in 1999. GES Core provides genomics and epigenomics related service and collaboration to researchers at the University of Cincinnati, Cincinnati Children's Hospital and other institutions.
In 2012, GES Core was transformed into the next generation sequencing-focused core facility. Since then, it has been expanding its services. Equipped with Illumina NestSeq 550 sequencer and automated liquid handling system, the Core provides a variety of high-quality NGS services. More recently, the Core introduced single cell sequencing via 10x Genomics Chromium and its supportive system such as LUNA-FL Automated Fluorescence Cell Counter and automated gentleMACS Octo Dissociator with Heaters from Miltenyi. In addition, it offers Illumina BeadChip methylationEPIC assay for epidemiology study.
The Core Director Xiang Zhang routinely provides consultation to researchers from sample preparation to data generation.
GES Core is one of the best equipped laboratories in the College. 100% of its expenses is paid from its service revenue. It is fully sustainable and always maintains financial stability over the past 9 years.
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RNA/DNA extraction (depending on sample type and number; human blood and clinical samples: $40).
Service rate is depdending on sample type and number. $40/sample for human blood and clinical samples.
Cost:Request Information$25 or $40
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10x Genomics single cell sequencing.
Cost:Request InformationPlease inquire.
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DNA/RNA Bioanalyzer QC (measure sample size distribution and integrity).
Cost:Request Information$25
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miRNA-seq (standard service; SR 1x85 bp, ~3M reads)
Cost:Request Information$240
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PolyA RNA-seq (standard service; SR1x85 bp, >20M pass filter reads)
Cost:Request Information$270
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Other types of sequencing (varies with experiment approach, kit/reagent, sequencing setting, etc).
Cost:Request InformationPlease inquire.
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MethylationEPIC/oxBS-methylationEPIC (extra charge for repeated assay (sample #: 8X) if the low data quality is not due to our mistake).
Cost:Request InformationPlease inquire.
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Other types of services (supportive DNA/RNA experiments).
Cost:Request InformationPlease inquire.
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Note 1: The rates are approved by UC Recharging Meeting. Due to the price change of kits, reagents, consumables, etc., they are subject to change without prior notice.
Cost:Request InformationFor inquiry, please email xiang.zhang@uc.edu.
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Note 2: Collaboration and/or co-authorship maybe required if the Core has significant intellectual contribution.
Request Information
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GES Core major equipment
GES Core has 12 benches, three fume hoods, three Revco high-performance ultra-low freezers, four Revco high-performance freezers, three refrigerators with backup. It is equipped with a series of instruments to support its service and research, from DNA/RNA isolation to library preparation and sequencing. This includes Illumina Nextseq 550 sequencing system and all the ancillary instruments such as Takara SMARTer Apollo system for automated liquid handling, ABI QuantStudio 5 Real-Time PCR System for library quantification, gene expression, genotyping and CNV analyses, Covaris S2 for precise size control of DNA fragmentation, Eppendorf Thermomixer block shakers with heating and cooling (two), incubators (two), Agilent Bioanalyzer and Invitrogen Qubit fluorometers (two) for DNA, RNA and library QC, Veriti thermal cycles (six) for enzyme reactions and DNA amplification, Labconco entriVap micro IR Vacuum Concentrator for DNA/RNA, and Labnet ENDURO GDS Touch Gel Documentation System agarose/PAGE gel electrophoresis systems for library/DNA/RNA analysis and size selection. It has a total of 24 different types of centrifuges to meet different experiment requirements. In addition, it is equipped with MPBio FastPrep-24 5G homogenizer for high quality RNA extraction from all types of samples. The Core has Illumina iScan and its ancillary instruments used in Infinium BeadChip System for epigenome-wide DNA methylation association study. Further, the Core has six different types of Schneider Electric Smart-UPS connected to its critical instruments to avoid potential power failure. For single cell sequencing, GES Core has 10x Genomics Chromium Single Cell System and its supportive system such as LUNA-FL Automated Fluorescence Cell Counter and fully automated gentleMACS Octo Dissociator with Heaters from Miltenyi.
Request Information