Riccardo Barrile

Riccardo Barrile

Assistant Professor

Positions and Work Experience

2017 -2018 Principal Investigator, Leading the research and development of enabling technologies for studying vascular hemostasis and thrombosis, lung cancer and a stem-cell derived in vitro model of the blood brain barrier for precision medicine applications , Emulate Inc., Boston (MA, USA)

2018 -2019 Associate Director, Establishment and oversee of multiple scientific collaborations with academic and pharma partners, which aimed toward the advancement and qualification of the Blood Vessel-on-Chip, Lung Alveolus-on-Chip and Liver-on-Chip models for detection of clinically relevant biomarkers associated with drug-induced toxicity., Emulate Inc., Boston (MA, USA)

Research Support

Grant: #N/A Investigators:Barrile, Riccardo 01-01-2021 -01-31-2021 UC's Launch Awards Research Launch Awards (January 2020 class of faculty) Role:PI $2,500.00 Active Level:Internal UC

Grant: #Project #2022-01 Alan and Helene Goldberg Investigators:Barrile, Riccardo 02-21-2022 -02-20-2024 Johns Hopkins University A Sensor-Powered Blood-Vessel on-Chip for Deciphering of COVID-19-Vaccine Induced Thrombosis and De-risking Future Antibody Therapies Role:PI 0.00 Hold Level:Higher Education

Grant: #CHMC CuSTOM Investigators:Barrile, Riccardo 02-01-2022 -01-31-2023 Cincinnati Children's Hospital Medical Center Bioengineering and organotypic human blood-brain barrier model Role:PI 25000.00 Hold Level:Non Profit

Grant: #OS00000851/401484 / 1R01HL167700-01A1 Investigators:Barrile, Riccardo 04-01-2024 -03-31-2028 Cincinnati Children's Hospital Medical Center Pathogenesis of Vascular Anomalies with GNAQ mutations Role:PI 26973.00 Hold Level:Non Profit

Grant: #Complement-ARIE Challenge Investigators:Barrile, Riccardo 06-01-2024 -05-31-2025 Office of the Director, National Institutes of Health Synergistic Integration of Patient-Derived Immunocompetent Assembloids into a Sensor-Enabled Microphysiological System for Advanced Neuroinflammation Studies Role:PI 50000.00 Hold Level:Federal


Peer Reviewed Publications

Jang, Kyung-Jin; Otieno, Monicah A; Ronxhi, Janey; Lim, Heng-Keang; Ewart, Lorna; Kodella, Konstantia R; Petropolis, Debora B; Kulkarni, Gauri; Rubins, Jonathan E; Conegliano, David; Nawroth, Janna; Simic, Damir; Lam, Wing; Singer, Monica; Barale, Erio; Singh, Bhanu; Sonee, Manisha; Streeter, Anthony J; Manthey, Carl; Jones, Barry; Srivastava, Abhishek; Andersson, Linda C; Williams, Dominic; Park, Hyoungshin; Barrile, Riccardo; Sliz, Josiah; Herland, Anna; Haney, Suzzette; Karalis, Katia; Ingber, Donald E; Hamilton, Geraldine A (2019. ) Reproducing human and cross-species drug toxicities using a Liver-Chip.Science translational medicine, , 11 (517 ) , More Information

Vatine, Gad D; Barrile, Riccardo; Workman, Michael J; Sances, Samuel; Barriga, Bianca K; Rahnama, Matthew; Barthakur, Sonalee; Kasendra, Magdalena; Lucchesi, Carolina; Kerns, Jordan; Wen, Norman; Spivia, Weston R; Chen, Zhaohui; Van Eyk, Jennifer; Svendsen, Clive N (2019. ) Human iPSC-Derived Blood-Brain Barrier Chips Enable Disease Modeling and Personalized Medicine Applications.Cell stem cell, , 24 (6 ) ,995-1005.e6 More Information

Nawroth, Janna C; Barrile, Riccardo; Conegliano, David; van Riet, Sander; Hiemstra, Pieter S; Villenave, Remi (2019. ) Stem cell-based Lung-on-Chips: The best of both worlds?.Advanced drug delivery reviews, , 140 ,12-32 More Information

Barrile, Riccardo; van der Meer, Andries D; Park, Hyoungshin; Fraser, Jacob P; Simic, Damir; Teng, Fang; Conegliano, David; Nguyen, Justin; Jain, Abhishek; Zhou, Mimi; Karalis, Katia; Ingber, Donald E; Hamilton, Geraldine A; Otieno, Monicah A (2018. ) Organ-on-Chip Recapitulates Thrombosis Induced by an anti-CD154 Monoclonal Antibody: Translational Potential of Advanced Microengineered Systems.Clinical pharmacology and therapeutics, , 104 (6 ) ,1240-1248 More Information

Jain, A; Barrile, R; van der Meer, A D; Mammoto, A; Mammoto, T; De Ceunynck, K; Aisiku, O; Otieno, M A; Louden, C S; Hamilton, G A; Flaumenhaft, R; Ingber, D E (2018. ) Primary Human Lung Alveolus-on-a-chip Model of Intravascular Thrombosis for Assessment of Therapeutics.Clinical pharmacology and therapeutics, , 103 (2 ) ,332-340 More Information

Jain, Abhishek; van der Meer, Andries D; Papa, Anne-Laure; Barrile, Riccardo; Lai, Angela; Schlechter, Benjamin L; Otieno, Monicah A; Louden, Calvert S; Hamilton, Geraldine A; Michelson, Alan D; Frelinger, Andrew L; Ingber, Donald E (2016. ) Assessment of whole blood thrombosis in a microfluidic device lined by fixed human endothelium.Biomedical microdevices, , 18 (4 ) ,73 More Information

Barrile, Riccardo; Kasendra, Magdalena; Rossi-Paccani, Silvia; Merola, Marcello; Pizza, Mariagrazia; Baldari, Cosima; Soriani, Marco; Aricò, Beatrice (2015. ) Neisseria meningitidis subverts the polarized organization and intracellular trafficking of host cells to cross the epithelial barrier.Cellular microbiology, , 17 (9 ) ,1365-75 More Information

Bozza, Giuseppe; Capitani, Mirco; Montanari, Paolo; Benucci, Barbara; Biancucci, Marco; Nardi-Dei, Vincenzo; Caproni, Elena; Barrile, Riccardo; Picciani, Benedetta; Savino, Silvana; Aricò, Beatrice; Rappuoli, Rino; Pizza, Mariagrazia; Luini, Alberto; Sallese, Michele; Merola, Marcello (2014. ) Role of ARF6, Rab11 and external Hsp90 in the trafficking and recycling of recombinant-soluble Neisseria meningitidis adhesin A (rNadA) in human epithelial cells.PloS one, , 9 (10 ) ,e110047 More Information

Casellato, Alessandro; Rossi Paccani, Silvia; Barrile, Riccardo; Bossi, Fleur; Ciucchi, Laura; Codolo, Gaia; Pizza, Mariagrazia; Aricò, Beatrice; de Bernard, Marina (2014. ) The C2 fragment from Neisseria meningitidis antigen NHBA increases endothelial permeability by destabilizing adherens junctions.Cellular microbiology, , 16 (6 ) ,925-37 More Information

Kasendra, Magdalena; Barrile, Riccardo; Leuzzi, Rosanna; Soriani, Marco (2014. ) Clostridium difficile toxins facilitate bacterial colonization by modulating the fence and gate function of colonic epithelium.The Journal of infectious diseases, , 209 (7 ) ,1095-104 More Information

Montanari, Paolo; Bozza, Giuseppe; Capecchi, Barbara; Caproni, Elena; Barrile, Riccardo; Norais, Nathalie; Capitani, Mirco; Sallese, Michele; Cecchini, Paola; Ciucchi, Laura; Gao, Zhenai; Rappuoli, Rino; Pizza, Mariagrazia; Aricò, Beatrice; Merola, Marcello (2012. ) Human heat shock protein (Hsp) 90 interferes with Neisseria meningitidis adhesin A (NadA)-mediated adhesion and invasion.Cellular microbiology, , 14 (3 ) ,368-85 More Information

Ng P.;Belgur C.;Barthakur S.;van der Meer A.;Hamilton G.;Barrile R. (10-01-2019. ) Organs-on-Chips: a new paradigm for safety assessment of drug-induced thrombosis.Current Opinion in Toxicology, , 17 ,1-8 More Information

Kerns, Jordan S; Belgur, Chaitra; Kanellias, Marianne; Manatakis, Dimitris V; Barrile, Riccardo; Tien-Street, Will; Ewart, Lorna; Gjorevski, Nikolche; Cabon, Lauriane (2023. ) Safety Profiling of Tumor-targeted T Cell-Bispecific Antibodies with Alveolus Lung- and Colon-on-Chip.Bio-protocol, , 13 (1 ) , More Information

Sawyer, Russell P; Pun, Sirjana; Karkoska, Kristine A; Clendinen, Cherita A; DeBaun, Michael R; Gutmark, Ephraim; Barrile, Riccardo; Hyacinth, Hyacinth I (2022. ) Effect of Blood Transfusion on Cerebral Hemodynamics and Vascular Topology Described by Computational Fluid Dynamics in Sickle Cell Disease Patients.Brain sciences, , 12 (10 ) , More Information

Pun, Sirjana; Haney, Li Cai; Barrile, Riccardo (2021. ) Modelling Human Physiology on-Chip: Historical Perspectives and Future Directions.Micromachines, , 12 (10 ) , More Information

Kerns, S Jordan; Belgur, Chaitra; Petropolis, Debora; Kanellias, Marianne; Barrile, Riccardo; Sam, Johannes; Weinzierl, Tina; Fauti, Tanja; Freimoser-Grundschober, Anne; Eckmann, Jan; Hage, Carina; Geiger, Martina; Ng, Patrick Ray; Tien-Street, William; Manatakis, Dimitris V; Micallef, Virginie; Gerard, Regine; Bscheider, Michael; Breous-Nystrom, Ekaterina; Schneider, Anneliese; Giusti, Anna Maria; Bertinetti-Lapatki, Cristina; Grant, Heather Shannon; Roth, Adrian B; Hamilton, Geraldine A; Singer, Thomas; Karalis, Katia; Moisan, Annie; Bruenker, Peter; Klein, Christian; Bacac, Marina; Gjorevski, Nikolce; Cabon, Lauriane (2021. ) Human immunocompetent Organ-on-Chip platforms allow safety profiling of tumor-targeted T-cell bispecific antibodies.eLife, , 10 , More Information

Varone, Antonio; Nguyen, Justin Ke; Leng, Lian; Barrile, Riccardo; Sliz, Josiah; Lucchesi, Carolina; Wen, Norman; Gravanis, Achille; Hamilton, Geraldine A; Karalis, Katia; Hinojosa, Christopher D (2021. ) A novel organ-chip system emulates three-dimensional architecture of the human epithelia and the mechanical forces acting on it.Biomaterials, , 275 ,120957 More Information

Thacker, Vivek V; Dhar, Neeraj; Sharma, Kunal; Barrile, Riccardo; Karalis, Katia; McKinney, John D (2020. ) A lung-on-chip model of early Mycobacterium tuberculosis infection reveals an essential role for alveolar epithelial cells in controlling bacterial growth.eLife, , 9 , More Information

Invited Publications

Patrick Ng, Chaitra Belgur, Sonalee Barthakur, Andries D. van der Meer, Geraldine A. Hamilton, Riccardo Barrile (2019. ) Organs-on-Chips: a new paradigm for safety assessment of drug-induced thrombosis.Current Opinion in Toxicology, 17 (2019 ) ,1 -8More Information

Post Graduate Training and Education

2011-2014 PhD in Developmental Biology., Investigating the cellular mechanism adopted by Neisseria meningitidis to colonize and penetrate the airway epithelium and the vascular blood brain barrier using in vitro relevant models., Novartis Vaccines and Diagnostics & University of Siena, , Siena, Italy

2015-2016 Postdoctoral Fellow, Development of a breathing human Alveolus Lung-Chip for studying of pulmonary thrombosis and monoclonal antibody induced toxicity, Wyss Institute for Biologically Inspired Engineering at Harvard Medical School, , Boston (MA, USA)

2016-2018 Postdoctoral Fellow, Development of a personalized model of the human blood-brain-barrier on-chip, Cedar Sinai Medical Centre, , Los Angeles (CA, USA)

Professional Affiliation

2017: Member of the International Blood Brain Barrier Society

Patents and Inventions

ca2987271 a1 Methods, systems, and compositions for determining blood clot formation, and uses thereof BAD DATE

20170285003 Devices, systems and methods for inhibiting invasion and metastases of cancer BAD DATE

20180017584 Additive channels BAD DATE

WO/2018/102201 In vitro epithelial models comprising lamina propria-derived cells BAD DATE

Other Information

Research Faculty memeber of the Center for Stem Cell & Organoid Medicine (CuSTOM) at the Cincinnati Children Hospital,