Dennis E Coyle , PhD

Bachelor's Degree: State University College at Brockport 1974 (Biology, Chemistry)

Doctoral Degree: University of Cincinnati 1980 (Chemistry)

Postdoctoral Fellowship: University of Cincinnati College of Medicine 1982 (Biochemistry)

Although animal models have greatly increased our understanding of the mechanisms involved in the disease process, the translation of this information to the human condition has had a high failure rate including the translation of animal data to human neurological diseases. Pain, in particular, has been highly disappointing since there are very few examples of analgesic drugs that have translated from animal studies alone. These outcomes suggest that the animal models currently in use need to be improved or that there are key differences between humans and animal models. Possible differences at the cellular level are the signal transduction pathways activated, the posttranslational modifications that result, and/or the alterations in gene transcription or translation. In order to address the differences in the molecular mechanisms, ex vivo human neural tissue is needed.  Human nervous tissue is rarely obtained, is present in limited quantities, and if diseased it is obtained at late stages of the disease. Another source of human neurons and non-neuronal brain cells (i.e., various glia cell types) is human embryonic stem cells (hES cells).  Most research on hES cells has focused on regeneration and development, yet the neurons obtained are both electrically active, end terminally differentiated, and have been shown to mature under in vitro conditions. This laboratory is testing the hypothesis that human central neurons differentiated in vitro from hES cells are a functionally and genetically representative model for human neurons and display cellular level differences from rodent animal models.
 

Peer Reviewed Publications

Blankenship, M L; Coyle, D E; Baccei, M L (2013. ) Transcriptional expression of voltage-gated Na? and voltage-independent K? channels in the developing rat superficial dorsal horn.Neuroscience, , 231 ,305-14 More Information

Strong, Judith A; Xie, Wenrui; Coyle, Dennis E; Zhang, Jun-Ming (2012. ) Microarray analysis of rat sensory ganglia after local inflammation implicates novel cytokines in pain.PloS one, , 7 (7 ) ,e40779 More Information

Coyle, Dennis E; Li, Jie; Baccei, Mark (2011. ) Regional differentiation of retinoic acid-induced human pluripotent embryonic carcinoma stem cell neurons.PloS one, , 6 (1 ) ,e16174 More Information

Wang, Jun-Gang; Strong, Judith A; Xie, Wenrui; Yang, Rui-Hua; Coyle, Dennis E; Wick, Dayna M; Dorsey, Ericka D; Zhang, Jun-Ming (2008. ) The chemokine CXCL1/growth related oncogene increases sodium currents and neuronal excitability in small diameter sensory neurons.Molecular pain, , 4 ,38 More Information

Coyle, Dennis E (2007. ) Spinal mechanisms of pain.International anesthesiology clinics, , 45 (2 ) ,83-94 More Information

Coyle, D E (2007. ) Spinal cord transcriptional profile analysis reveals protein trafficking and RNA processing as prominent processes regulated by tactile allodynia.Neuroscience, , 144 (1 ) ,144-56 More Information

Coyle, D E (1998. ) Partial peripheral nerve injury leads to activation of astroglia and microglia which parallels the development of allodynic behavior. Glia, , 23 (1 ) ,75-83

Coyle, D E; Sehlhorst, C S; Behbehani, M M (1996. ) Intact female rats are more susceptible to the development of tactile allodynia than ovariectomized female rats following partial sciatic nerve ligation (PSNL). Neuroscience letters, , 203 (1 ) ,37-40

Coyle, D E (1995. ) Adaptation of C6 glioma cells to serum-free conditions leads to the expression of a mixed astrocyte-oligodendrocyte phenotype and increased production of neurite-promoting activity.Journal of neuroscience research, , 41 (3 ) ,374-85 More Information

Coyle, D E; Sehlhorst, C S; Mascari, C (1995. ) Female rats are more susceptible to the development of neuropathic pain using the partial sciatic nerve ligation (PSNL) model. Neuroscience letters, , 186 (2-3 ) ,135-8

Coyle, D E; Porembka, D T; Sehlhorst, C S; Wan, L; Behbehani, M M (1994. ) Echocardiographic evaluation of bupivacaine cardiotoxicity. Anesthesia and analgesia, , 79 (2 ) ,335-9

Coyle, D E (1993. ) Identification of a collagen potentiated neurite promoting factor isolated from C6 glioma cells.Journal of neuroscience research, , 35 (4 ) ,390-401 More Information

Wahler, G M; Coyle, D E; Sperelakis, N (1990. ) Effects of platelet-activating factor on single potassium channel currents in guinea pig ventricular myocytes. Molecular and cellular biochemistry, , 93 (1 ) ,69-76

Parab, P V; Ritschel, W A; Coyle, D E; Gregg, R V; Denson, D D Pharmacokinetics of hydromorphone after intravenous, peroral and rectal administration to human subjects. Biopharmaceutics & drug disposition, , 9 (2 ) ,187-99

Ritschel, W A; Parab, P V; Denson, D D; Coyle, D E; Gregg, R V (1987. ) Absolute bioavailability of hydromorphone after peroral and rectal administration in humans: saliva/plasma ratio and clinical effects. Journal of clinical pharmacology, , 27 (9 ) ,647-53

Coyle, D E; Sperelakis, N (1987. ) Bupivacaine and lidocaine blockade of calcium-mediated slow action potentials in guinea pig ventricular muscle. The Journal of pharmacology and experimental therapeutics, , 242 (3 ) ,1001-5

Parab, P V; Ritschel, W A; Coyle, D E; Denson, D D (1987. ) Comparison of pharmacokinetic parameters determined by four different methods. Methods and findings in experimental and clinical pharmacology, , 9 (4 ) ,251-60

Thompson, G A; Myers, J A; Turner, P A; Coyle, D E; Ritschel, W A; Denson, D D Influence of age on intrinsic clearance of bupivacaine and its reduction by cimetidine in elderly male rats. Drug metabolism and disposition: the biological fate of chemicals, , 15 (1 ) ,136-7

Thomas, R D; Behbehani, M M; Coyle, D E; Denson, D D (1986. ) Cardiovascular toxicity of local anesthetics: an alternative hypothesis. Anesthesia and analgesia, , 65 (5 ) ,444-50

Coyle, D E; Denson, D D; Essell, S K; Santos, D J (1986. ) The effect of nonesterified fatty acids and progesterone on bupivacaine protein binding. Clinical pharmacology and therapeutics, , 39 (5 ) ,559-63

Denson, D D; Thompson, G A; Coyle, D E Use of a limited physiologic model to explain target organ toxicity of bupivacaine as a function of route of administration. Biopharmaceutics & drug disposition, , 7 (2 ) ,121-35

Coyle, D E; Denson, D D (1986. ) Simultaneous measurement of bupivacaine, etidocaine, lidocaine, meperidine, mepivacaine, and methadone. Therapeutic drug monitoring, , 8 (1 ) ,98-101

Coyle, D E; Park, R E (1985. ) Effect of different acidification on in vitro human serum bupivacaine binding. Biochemical pharmacology, , 34 (20 ) ,3779-81

Denson, D D; Myers, J A; Thompson, G A; Coyle, D E (1984. ) The influence of diazepam on the serum protein binding of bupivacaine at normal and acidic pH. Anesthesia and analgesia, , 63 (11 ) ,980-4

Coyle, D E; Denson, D D Protein binding of lidocaine in canine serum and plasma: effects of an acidic pH and the technique of study. Biopharmaceutics & drug disposition, , 5 (4 ) ,399-404

Hartrick, C T; Dirkes, W E; Coyle, D E; Raj, P P; Denson, D D (1984. ) Influence of bupivacaine on mepivacaine protein binding. Clinical pharmacology and therapeutics, , 36 (4 ) ,546-50

Thompson, G A; Myers, J A; Turner, P A; Denson, D D; Coyle, D E; Ritschel, W A Influence of cimetidine on bupivacaine disposition in rat and monkey. Drug metabolism and disposition: the biological fate of chemicals, , 12 (5 ) ,625-30

Denson, D D; Myers, J A; Coyle, D E (1984. ) The clinical relevance of the drug displacement interaction between meperidine and bupivacaine. Research communications in chemical pathology and pharmacology, , 45 (3 ) ,323-30

Coyle, D E; Denson, D D; Thompson, G A; Myers, J A; Arthur, G R; Bridenbaugh, P O (1984. ) The influence of lactic acid on the serum protein binding of bupivacaine: species differences. Anesthesiology, , 61 (2 ) ,127-33

Denson, D D; Coyle, D E; Thompson, G A; Santos, D; Turner, P A; Myers, J A; Knapp, R (1984. ) Bupivacaine protein binding in the term parturient: effects of lactic acidosis. Clinical pharmacology and therapeutics, , 35 (5 ) ,702-9

Ford, D J; Coyle, D E; Harrington, J F (1984. ) Effects of hypersensitivity to a halothane metabolite on halothane-induced liver damage. Anesthesiology, , 60 (2 ) ,141-3

Coyle, D E; Brammer, P W; Halsall, H B (1980. ) A microcapillary assay (MASS) for macrophage migration inhibition factor. Journal of immunological methods, , 35 (3-4 ) ,259-65

Ikeda R, Cha M, Ling J, Jia Z, Coyle DE, Gu JG. (2014. ) Merkel cells transduce and encode tactile stimuli to drive Aβ-afferent impulses.

Electronic Journal

Coyle DE (2008. ) Real time quantification of gene expression of NTERA2 neurons confirms differentiation consistent with the CNS neuronal cell type. .Society for Neuroscience, 25 (3 ) ,

Neuropathic Pain ,Pharmacokinetics(MeSH PhrmKin),Anatomy(MeSH A),Chemicals and Drugs(MeSH D),Analytical, Diagnostic and Therapeutic Techniques and Equipment(MeSH E),Biological Sciences(MeSH G),Animal Structures(MeSH A13),Vertebrates(MeSH B02),Nucleic Acids, Nucleotides, and Nucleosides(MeSH D13),Peripheral Nervous System Agents(MeSH D16),Investigative Techniques(MeSH E05),Chemical and Pharmacologic Phenomena(MeSH G12)

Academic - Medical Sciences Building
Room 3205
Cincinnati  Ohio, 45267
Phone: 513-558-4194
Fax: 513-558-0995
dennis.coyle@uc.edu