John Cuppoletti, PhD

Grant: #CR-19275-430099 Investigators:Cuppoletti, John 07-01-2004 -11-30-2006 Department of the Army High Energy Density Nastic Structure Utilizing Biological Transport Mechanisms Role:PI $800,000.00 Closed Level:Federal

Investigators:Cuppoletti, John 08-01-2005 -07-31-2006 Monsanto Company Monsanto Cry Project Role:PI $102,000.00 Closed Level:Industry

Grant: #1003399 Investigators:Cuppoletti, John 02-01-2005 -12-31-2011 Sucampo Pharmaceuticals, Inc Rescula Role:PI $2,649,999.00 Closed Level:Industry

Grant: #W911NF-04-1-0165 Investigators:Cuppoletti, John 05-01-2004 -04-30-2005 Department of Defense High Pressure Suite for Studies of Structures and Function of Molecules and Membranes Role:PI $197,400.00 Closed Level:Federal

Investigators:Cuppoletti, John 07-01-2004 -06-30-2005 Department of Defense Symposia on Biophysical and Biochemical Properties on Ion Channels in Epithelia: Funding for Young Investigators Role:PI $5,000.00 Closed Level:Federal

Investigators:Cuppoletti, John 05-15-2004 -11-30-2005 Air Force Office of Scientific Research Laboratory Scale Production of Membrane Vesicles Role:PI $19,999.00 Closed Level:Federal

Investigators:Cuppoletti, John 09-01-2004 -02-28-2005 Sucampo Pharmaceuticals, Inc SAG-017 Role:PI $96,705.00 Closed Level:Industry

Grant: #CFF R 457-02-A0-S0-E0 Investigators:Cuppoletti, John 09-01-2000 -08-31-2002 Cystic Fibrosis Foundation Cl Channel Replacement for CFTR in CF Role:PI $80,000.00 Closed Level:Private Non-Profit

Investigators:Cuppoletti, John 08-01-2001 -03-31-2003 Sucampo Pharmaceuticals, Inc Exploratory Studies on RU0211 and RU8811 Role:PI $132,377.00 Closed Level:Industry

Grant: #2002-UC-29-CFF-01-A0-S0-E0 Investigators:Cuppoletti, John 12-01-2001 -05-31-2003 Cystic Fibrosis Foundation Role of Regulated CIC Ci Channels in CI Secretion in CF Role:PI $100,000.00 Closed Level:Private Non-Profit

Grant: #DAAD19-02-1-0227 Investigators:Beck, Thomas; Boerio, F James; Cuppoletti, John; Lin, Jerry Y.S.; Rosevear, Paul 06-10-2002 -06-09-2007 Department of the Army Flexible Membranes for Active Transport of HCl Role:PI $4,999,999.39 Closed Level:Federal

Grant: #R01-HL-58399 Investigators:Cuppoletti, John 12-15-2002 -11-30-2007 National Heart, Lung and Blood Institute Role of Regulated CIC Channels in CI Secretion in Cystic Fibrosis Role:PI $1,296,634.00 Closed Level:Federal

Grant: #5-R01-HL-57614-04-A0-S0-E0 Investigators:Cuppoletti, John 09-01-1997 -08-31-2001 National Heart, Lung and Blood Institute Inhibition of Ion Transport in Deoxygenated Sickle Cells Role:PI $66,956.00 Closed Level:Federal

Grant: #5-R01-HL-58399-04-A0-S0-E0 Investigators:Cuppoletti, John 07-20-1997 -12-14-2002 National Heart, Lung and Blood Institute Regulated CLC Cl Channels and Cl Secretion and CF Role:PI $1,039,338.00 Closed Level:Federal

Grant: #5-R01-DK-43816-13-A0-S0-E0 Investigators:Cuppoletti, John 05-01-1999 -04-30-2006 National Inst of Diabetes and Digestive and Kidney Disease Chloride Channels and Gastric Acid Secretion Role:PI $1,598,324.00 Closed Level:Federal

Grant: #AFOSR FA9550-07-1-0257 Investigators:Cuppoletti, John; Malinowska, Danuta 03-15-2007 -11-30-2009 Air Force Office of Scientific Research Mechanisms of Ion Transport and Barrier Function of Membranes at Extremes of Temperature and Ionic Conditions Role:PI $450,000.00 Closed Level:Federal

Grant: #UDRI RSC 07028-ODOD W944SR-06-C-0043 Investigators:Cuppoletti, John; Heikenfeld, Jason; Heineman, William; Steckl, Andrew 02-26-2007 -02-26-2014 Ohio Department of Development Institute for Development Commercialization of Advanced Sensor Role:Collaborator $1,000,000.00 Active Level:State of Ohio

Grant: #SRS007565 Investigators:Cuppoletti, John 05-01-2011 -07-31-2016 Sucampo Pharmaceuticals, Inc Prostone Research for Sucampo Role:PI $1,359,615.00 Active Level:Industry

Published Abstracts

Cuppoletti J, Malinowska DH, Tewari KP, Chakrabarti J & Ueno R (2010) Methadone, but not morphine inhibits stimulated chloride currents in ClC-2 transfected cells in culture. DDW 2010, New Orleans LA, May 2010. . [Abstract]

Cuppoletti J, Malinowska DH, Chakrabarti J, Sekida T & Ueno R (2010) Lubiprostone suppresses growth of colon cancer cells in vitro and in vivo. Am College of Gastroenterology meeting 2010, San Antonio TX, October 2010. [Abstract]

Cuppoletti J, Malinowska DH & Chakrabarti J (2010) Lubiprostone, but not linoclatide, protects T84 cells against damage to barrier function by interferon-γ. Am College of Gastroenterology meeting 2010, San Antonio TX, October 2010.. [Abstract]

Peer Reviewed Publications

Cuppoletti, John; Chakrabarti, Jayati; Tewari, Kirti P; Malinowska, Danuta H  (2014). Differentiation between human ClC-2 and CFTR Cl- channels with pharmacological agents. American journal of physiology. Cell physiology, 307 (5) , C479-92More Information

Cuppoletti, John; Chakrabarti, Jayati; Tewari, Kirti; Malinowska, Danuta H  (2013). Methadone but not morphine inhibits lubiprostone-stimulated Cl- currents in T84 intestinal cells and recombinant human ClC-2, but not CFTR Cl- currents. Cell biochemistry and biophysics, 66 (1) , 53-63More Information

Duan, Yuanyuan; Sun, Ying; Zhang, Fan; Zhang, Wei Kevin; Wang, Dong; Wang, Yan; Cao, Xu; Hu, Wenbao; Xie, Changyan; Cuppoletti, John; Magin, Thomas M; Wang, Haixia; Wu, Zhenguo; Li, Ning; Huang, Pingbo  (2012). Keratin K18 increases cystic fibrosis transmembrane conductance regulator (CFTR) surface expression by binding to its C-terminal hydrophobic patch. The Journal of biological chemistry, 287 (48) , 40547-59More Information

Cuppoletti, John; Malinowska, Danuta H; Tewari, Kirti P; Chakrabarti, Jayati; Ueno, Ryuji  (2012). Unoprostone isopropyl and metabolite M1 activate BK channels and prevent ET-1-induced [Ca²?]i increases in human trabecular meshwork and smooth muscle. Investigative ophthalmology & visual science, 53 (9) , 5178-89More Information

Cuppoletti, John; Blikslager, Anthony T; Chakrabarti, Jayati; Nighot, Prashant K; Malinowska, Danuta H  (2012). Contrasting effects of linaclotide and lubiprostone on restitution of epithelial cell barrier properties and cellular homeostasis after exposure to cell stressors. BMC pharmacology, 12, 3More Information

Szyma?ska, G; Kim, H W; Cuppoletti, J; Kranias, E G  (1992). Regulation of the skeletal sarcoplasmic reticulum Ca(2+)-ATPase by phospholamban and negatively charged phospholipids in reconstituted phospholipid vesicles. Molecular and cellular biochemistry, 114 (1-2) , 65-71

Szyma?ska, G; Kim, H W; Cuppoletti, J; Kranias, E GRegulation of the skeletal sarcoplasmic reticulum Ca2+ pump by phospholamban in reconstituted phospholipid vesicles. Membrane biochemistry, 9 (3) , 191-202

Other Publications

Ueno R, Kuno S, Osama H, Cuppoletti J, Roerig B, Dolocek G & Sekida T (2010) Physiological and Therapeutic Effects of Lubiprostone, a ClC-2 Chloride Channel Activator, Do Not Involve Prostaglandin Receptors. Journal of European Pharmacology. .

Cuppoletti J & Malinowska DH (2010) Ion channels of the epithelia of the gastrointestinal tract. Physiology of the Gastrointestinal Tract, ed. Johnson LR, Elsevier .

Cuppoletti J, Chakrabarti J, Tewari KP & Malinowska DH (2010) Methadone, but not morphine inhibits stimulated chloride currents in ClC-2 transfected cells in culture. Am J Physiol Cell .

Cuppoletti J, Chakrabarti J & Malinowska DH. (2010) Lubiprostone, but not linoclatide, protects T84 cells against damage to barrier function by interferon-γ. POLM. .

American Journal of Physiology Cell Editorial Board Member Type:Editorial Service Level:National 1992

AAUP President Type:Public Level:State 2010 -2012

20070026217 Selectively permeable film supported membrane Cuppoletti; John , Awarded 06-16-2006 Inventor

20070116610 Selectively permeable membranes on porous substrates Cuppoletti; John , Awarded 05-24-2007 Inventor

20040038421 Methods for studying ion channels incorporated onto a solid-supported membrane Cuppoletti, John , 02-26-2004 Inventor