Melanie Cushion

Melanie T Cushion

Senior Associate Dean for Research

Medical Sciences Building

870

COM Dean Office of Research - 0555

CARE / Crawley Building
Room E870
Cincinnati, Ohio 45267

Professional Summary

 Professor Cushion is a bench scientist with funding from the NIH, VA and other sources. She has been named as Research Career Scientist in the VA system for two consecutive five-year terms and is a member of the Academy of Microbiology. Her research focuses on the fungal pathogens in the species, Pneumocystis.  These fungi can cause a lethal pneumonia in patients and animals whose immune systems have become debilitated by infection (e.g. HIV in humans), chemotherapy, and anti-rejection drugs such as corticosteroids.  Her approaches to investigate these enigmatic fungi include analysis of their life cycle including mode of transmission, preclinical drug development, and  the genomics of Pneumocystis.  She directs the Pneumocystis Genome Project (http://pgp.cchmc.org)
 
She has extensive experience in academic administration. She has participated in training in executive leadership through the ELAM program (Executive Leadership in Academic Medicine).  As Associate Chair for Research in the Department of Internal Medicine, Professor Cushion has organized symposia, administered grants and is currently participating in an effort to create a Research Core infrastructure to aid investigators in their research at every step of the process. Most recently, she proposed and launched a university-wide series of workshops that provide necessary skills to enable research faculty to establish a successful laboratory and research program.
 
Her entrepreneurial experience includes holding a patent and having launched asmall business initiative for pre-clinical drug development and other research services.
 
She has always had an interest in the advancement of women and increasing diversity at UC.  In a departure from her laboratory research, she collaborated with colleagues from other Colleges to seek NSF funding for an ADVANCE program at the University of Cincinnati.  The submission has been recommended for funding and this new program,  LEAF (Leadership, Empowerment, and Advancement of Women STEM Faculty) will be launched shortly. LEAF will address inequities of women faculty in STEM at UC.

Education

Bachelor's Degree, St. Joseph's College 1977 (Biology-Chemistry)

Doctoral Degree, St. Thomas Institute 1981 (The biological and immunological activities of phospholipids)

Fellowship, University of Cincinnati College of Medicine 1985

Research Support

Investigators:Cushion, Melanie 02-01-2004 -01-31-2005 National Institute of Allergy and Infectious Diseases Pneumocystis Genome Project Role:PI Closed 003365-001 Level:Federal

Grant: #R01 AI50450 Investigators:Cushion, Melanie 06-01-2002 -05-31-2008 National Institute of Allergy and Infectious Diseases New Approaches for Development of PcP Therapy Role:PI Closed 004110-001 Level:Federal

Grant: #5-R01-AI-44651-05-A0-S0-E0 Investigators:Cushion, Melanie 02-15-1999 -01-31-2005 National Institute of Allergy and Infectious Diseases Pneumocystis Genome Project Role:PI Closed 004550-001 Level:Federal

Grant: #R01 AI076104 Investigators:Cushion, Melanie 02-01-2008 -01-31-2012 National Institute of Allergy and Infectious Diseases Biofilm Formation by Pneumocystis Role:PI Active 005446-001 Level:Federal

Grant: #HHSN2722010000291 Investigators:Cushion, Melanie; Newman, Simon; Rhodes, Judith; Smulian, Alan; Walzer, Peter 03-22-2010 -03-21-2017 National Institute of Allergy and Infectious Diseases Animal Models of Infectious Diseases Role:PI $544,424.00 Active Level:Federal

Grant: #G1200050 \ R01 AI098458 Investigators:Cushion, Melanie 02-01-2012 -01-31-2017 National Institute of Allergy and Infectious Diseases Pneumocystis Jirovecii Targeted Antiopportunistics Agents Role:PI $49,361.00 Active Level:Federal

Grant: #111277060-7489196 \ 2 R01AI070721-06 Investigators:Cushion, Melanie 07-15-2012 -06-30-2014 National Institute of Allergy and Infectious Diseases Novel Inhibitors to DHPS to Probe Catalytic Mechanism & Therapeutic Potential Role:PI $96,180.00 Active Level:Federal

Grant: #HRD-1209169 Investigators:Cushion, Melanie; Degen, Sandra; Ghia, Urmila; Hardcastle, Valerie; Holloway, Stacie; Howe, Steven; Kallen, Rachel; Ono, Santa 09-15-2012 -08-31-2017 National Science Foundation Leadership, Empowerment, and Advancement for STEM Women Faculty (LEAF) at UC Role:Collaborator $738,310.00 Active Level:Federal

Grant: #R01 HL119190 Investigators:Cushion, Melanie; Medvedovic, Mario; Porollo, Alexey 05-22-2013 -02-28-2018 National Heart, Lung and Blood Institute Directed Culturing of Pneumocystis Using Metatranscriptomics Role:PI $404,514.00 Active Level:Federal

Grant: #HRD-1209169 Investigators:Cushion, Melanie; Degen, Sandra; Ghia, Urmila; Hardcastle, Valerie; Holloway, Stacie; Howe, Steven; Kallen, Rachel; Ono, Santa 09-15-2012 -08-31-2017 National Science Foundation Leadership, Empowerment, and Advancement for STEM Women Faculty (LEAF) at UC Role:Collaborator $1,468,854.00 Active Level:Federal

Grant: #SUNY-Stonybrook sub R01AI116420 Investigators:Cushion, Melanie 08-01-2016 -11-30-2020 National Institute of Allergy and Infectious Diseases Identification of new compounds against fungal microbes Role:PI $99,815.00 Awarded Level:Federal

Grant: #UTHSCA sub NIAID HHSN272201100018I Task A22 Investigators:Cushion, Melanie 08-01-2016 -07-31-2017 National Institute of Allergy and Infectious Diseases Development of a New System for Scaled Up Culture and Propagation of Pneumocystis Role:PI $119,826.00 Active Level:Federal

Grant: #HHSN272201700034I (Master Agreement) Investigators:Cushion, Melanie; Desai, Pankaj; Sallans, Larry 07-14-2017 -07-13-2024 National Institute of Allergy and Infectious Diseases PRE-CLINICAL MODELS OF INFECTIOUS DISEASES: Task Area A: Small Animal Models of Infectious Diseases Role:PI $.00 Active Level:Federal

Grant: #R13AI1134000 Investigators:Cushion, Melanie 08-08-2017 -07-31-2018 National Institute of Allergy and Infectious Diseases International Workshop on Opportunistic Protists (IWOP-14) Role:PI $5,000.00 Active Level:Federal

Grant: #BWF 1011031 Investigators:Ball, William; Cushion, Melanie; Way, Sing_Sing 10-01-2012 -09-30-2020 Burroughs Wellcome Fund Maternal Regulatory T cells Control the Immune Pathogenesis of Prenatal Infection Role:PI $500,000.00 Active Level:Private Non-Profit

Grant: #75N93019D00017 Investigators:Cushion, Melanie 01-15-2019 -01-14-2026 National Institute of Allergy and Infectious Diseases In Vitro Assessments of Antimicrobial Activity Role:PI $30,000.00 Active Level:Federal

Grant: #1 R01 HL146266-01 Investigators:Cushion, Melanie 02-01-2019 -01-31-2023 National Heart, Lung and Blood Institute The role of sex in the life cycle and transmission of Pneumocystis Role:PI $489,101.00 Awarded Level:Federal

Grant: #1012534 Investigators:Ball, William; Chougnet, Claire; Cushion, Melanie 06-01-2013 -03-31-2019 Burroughs Wellcome Fund Host-Microbe Cross-Talk and Pregnancy Outcomes Role:PI $600,000.00 Active Level:Private Non-Profit

Grant: #1013330.01 Investigators:Alenghat, Theresa; Ball, William; Cushion, Melanie 09-01-2014 -08-31-2019 Burroughs Wellcome Fund Epigenomic Regulation of the Host-commensal Relationship Role:PI $700,000.00 Active Level:Private Non-Profit

Grant: #1015032 Investigators:Ball, William; Cushion, Melanie; Divanovic, Senad 06-01-2015 -03-31-2019 Burroughs Wellcome Fund Pre-term Birth Initiative - Senad Divanovic Role:PI $600,000.00 Active Level:Private Non-Profit

Grant: #1017289 Investigators:Ball, William; Cushion, Melanie; Zhang, Ge 06-01-2017 -03-31-2021 Burroughs Wellcome Fund Genomic study of gestational length and preterm birth Role:PI $600,000.00 Active Level:Private Non-Profit

Grant: #1019095 Investigators:Cushion, Melanie 07-01-2019 -06-30-2024 Burroughs Wellcome Fund Dietary regulation of innate intestinal immunity Role:PI $500,000.00 Awarded Level:Private Non-Profit

Grant: #Joint Faculty Program MASTER Investigators:Cushion, Melanie 04-05-2019 -04-04-2024 Department of Energy UT Battelle Joint Faculty Program (Oak Ridge National Laboratory) Role:PI $.00 Awarded Level:Federal

Grant: #UT Battelle Subcontract 4000173601 (MASTER 012456) Investigators:Cushion, Melanie 07-28-2019 -11-28-2019 Department of Energy UT Battelle Joint Faculty Program (Oak Ridge National Laboratory) Role:PI $30,000.00 Awarded Level:Federal

Publications

Peer Reviewed Publications

Cushion, Melanie T; Stringer, James R (2010. )Stealth and opportunism: alternative lifestyles of species in the fungal genus Pneumocystis.Annual review of microbiology, ,64 ,431-52

Joffrion, Tiffany M; Cushion, Melanie T (2010. )Sterol biosynthesis and sterol uptake in the fungal pathogen Pneumocystis carinii.FEMS microbiology letters, ,311 (1 ),1-9

Cushion, Melanie T (2010. )Are members of the fungal genus pneumocystis (a) commensals; (b) opportunists; (c) pathogens; or (d) all of the above?.PLoS pathogens, ,6 (9 ),

Cushion, Melanie T; Linke, Michael J; Ashbaugh, Alan; Sesterhenn, Tom; Collins, Margaret S; Lynch, Keeley; Brubaker, Ronald; Walzer, Peter D (2010. )Echinocandin treatment of pneumocystis pneumonia in rodent models depletes cysts leaving trophic burdens that cannot transmit the infection.PloS one, ,5 (1 ),e8524

Joffrion, Tiffany M; Collins, Margaret S; Sesterhenn, Thomas; Cushion, Melanie T (2010. )Functional characterization and localization of Pneumocystis carinii lanosterol synthase.Eukaryotic cell, ,9 (1 ),107-15

Sesterhenn, Thomas M; Slaven, Bradley E; Keely, Scott P; Smulian, A George; Lang, B Franz; Cushion, Melanie T (2010. )Sequence and structure of the linear mitochondrial genome of Pneumocystis carinii.Molecular genetics and genomics : MGG, ,283 (1 ),63-72

Vale, Nuno; Prudêncio, Miguel; Marques, Catarina A; Collins, Margaret S; Gut, Jiri; Nogueira, Fátima; Matos, Joana; Rosenthal, Philip J; Cushion, Melanie T; do Rosário, Virgílio E; Mota, Maria M; Moreira, Rui; Gomes, Paula (2009. )Imidazoquines as antimalarial and antipneumocystis agents.Journal of medicinal chemistry, ,52 (23 ),7800-7

Huang, Tien L; Vanden Eynde, Jean Jacques; Mayence, Annie; Collins, Margaret S; Cushion, Melanie T; Rattendi, Donna; Londono, Indira; Mazumder, Lakshman; Bacchi, Cyrus J; Yarlett, Nigel (2009. )Synthesis and SAR of alkanediamide-linked bisbenzamidines with anti-trypanosomal and anti-pneumocystis activity.Bioorganic & medicinal chemistry letters, ,19 (20 ),5884-6

Brubaker, R; Redhead, S A; Stringer, J R; Keely, S P; Cushion, M T (2009. )Misinformation about Pneumocystis.Clinical and experimental dermatology, ,34 (7 ),e426-7

Beck, James M; Cushion, Melanie T (2009. )Pneumocystis workshop: 10th anniversary summary.Eukaryotic cell, ,8 (4 ),446-60

Cushion, Melanie T; Collins, Margaret S; Linke, Michael J (2009. )Biofilm formation by Pneumocystis spp.Eukaryotic cell, ,8 (2 ),197-206

Cushion, Melanie T; Walzer, Peter D (2009. )Preclinical drug discovery for new anti-pneumocystis compounds. Current medicinal chemistry, ,16 (20 ),2514-30

Liu, Yu; Leigh, Jessica W; Brinkmann, Henner; Cushion, Melanie T; Rodriguez-Ezpeleta, Naiara; Philippe, Hervé; Lang, B Franz (2009. )Phylogenomic analyses support the monophyly of Taphrinomycotina, including Schizosaccharomyces fission yeasts.Molecular biology and evolution, ,26 (1 ),27-34

Mayence, Annie; Pietka, Aurélie; Collins, Margaret S; Cushion, Melanie T; Tekwani, Babu L; Huang, Tien L; Vanden Eynde, Jean Jacques (2008. )Novel bisbenzimidazoles with antileishmanial effectiveness.Bioorganic & medicinal chemistry letters, ,18 (8 ),2658-61

Vale, Nuno; Collins, Margaret S; Gut, Jiri; Ferraz, Ricardo; Rosenthal, Philip J; Cushion, Melanie T; Moreira, Rui; Gomes, Paula (2008. )Anti-Pneumocystis carinii and antiplasmodial activities of primaquine-derived imidazolidin-4-ones.Bioorganic & medicinal chemistry letters, ,18 (2 ),485-8

Keely, Scott P; Linke, Michael J; Cushion, Melanie T; Stringer, James R (2007. )Pneumocystis murina MSG gene family and the structure of the locus associated with its transcription.Fungal genetics and biology : FG & B, ,44 (9 ),905-19

Cushion, Melanie T; Smulian, A George; Slaven, Bradley E; Sesterhenn, Tom; Arnold, Jonathan; Staben, Chuck; Porollo, Aleksey; Adamczak, Rafal; Meller, Jarek (2007. )Transcriptome of Pneumocystis carinii during fulminate infection: carbohydrate metabolism and the concept of a compatible parasite.PloS one, ,2 (5 ),e423

Cushion,M.T.; Smulian,A.G.; Slaven,B.E.; Sesterhenn,T.; Arnold,J.; Staben,C.; Porollo,A.; Adamczak,R.; Meller,J. (2007. )Transcriptome of Pneumocystis carinii during fulminate infection: carbohydrate metabolism and the concept of a compatible parasite .PLoS ONE, ,2 ,e423

Keely,S.P.; Linke,M.J.; Cushion,M.T.; Stringer,J.R. (2007. )Pneumocystis murina MSG gene family and the structure of the locus associated with its transcription .Fungal genetics and biology : FG & B, ,

Keely,S.P.; Linke,M.J.; Cushion,M.T.; Stringer,J.R. (2007. )Pneumocystis murina MSG gene family and the structure of the locus associated with its transcription .Fungal genetics and biology : FG & B, ,44 (9 ),905 -919

Slaven,B.E.; Meller,J.; Porollo,A.; Sesterhenn,T.; Smulian,A.G.; Cushion,M.T. (2006. )Draft assembly and annotation of the Pneumocystis carinii genome .The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S89 -91

Cushion,M.T.; Smulian,A.G. (2006. )Pneumocystis 2006: summary of the research presented at the ninth International Workshop on Opportunistic Protists .The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S80 -4

Sesterhenn,T.M.; Cushion,M.T.; Slaven,B.E.; Smulian,A.G. (2006. )Sequence of the mitochondrial genome of Pneumocystis carinii: implications for biological function and identification of potential drug targets .The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S154 -5

Slaven,B.E.; Porollo,A.; Sesterhenn,T.; Smulian,A.G.; Cushion,M.T.; Meller,J. (2006. )Large-scale characterization of introns in the Pneumocystis carinii genome .The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S151 -3

Joffrion,T.M.; Collins,M.S.; Cushion,M.T. (2006. )Microaerophilic conditions increase viability and affect responses of Pneumocystis carinii to drugs in vitro .The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S117 -8

Cushion,M.T.; Walzer,P.D.; Ashbaugh,A.; Rebholz,S.; Brubaker,R.; Vanden Eynde,J.J.; Mayence,A.; Huang,T.L. (2006. )In vitro selection and in vivo efficacy of piperazine- and alkanediamide-linked bisbenzamidines against Pneumocystis pneumonia in mice .Antimicrobial Agents and Chemotherapy, ,50 (7 ),2337 -2343

Redhead,S.A.; Cushion,M.T.; Frenkel,J.K.; Stringer,J.R. (2006. )Pneumocystis and Trypanosoma cruzi: nomenclature and typifications .The Journal of eukaryotic microbiology, ,53 (1 ),2 -11

Icenhour,C.R.; Arnold,J.; Medvedovic,M.; Cushion,M.T. (2006. )Competitive coexistence of two Pneumocystis species .Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, ,6 (3 ),177 -186

Cushion, Melanie T; Walzer, Peter D; Ashbaugh, Alan; Rebholz, Sandra; Brubaker, Ronald; Vanden Eynde, Jean Jacques; Mayence, Annie; Huang, Tien L (2006. )In vitro selection and in vivo efficacy of piperazine- and alkanediamide-linked bisbenzamidines against Pneumocystis pneumonia in mice.Antimicrobial agents and chemotherapy, ,50 (7 ),2337-43

Icenhour, Crystal R; Arnold, Jonathan; Medvedovic, Mario; Cushion, Melanie T (2006. )Competitive coexistence of two Pneumocystis species.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, ,6 (3 ),177-86

Slaven, Bradley E; Meller, Jaroslaw; Porollo, Aleksey; Sesterhenn, Thomas; Smulian, A George; Cushion, Melanie T (2006. )Draft assembly and annotation of the Pneumocystis carinii genome.The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S89-91

Cushion, Melanie T; Smulian, A George (2006. )Pneumocystis 2006: summary of the research presented at the ninth International Workshop on Opportunistic Protists.The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S80-4

Sesterhenn, Thomas M; Cushion, Melanie T; Slaven, Bradley E; Smulian, A George (2006. )Sequence of the mitochondrial genome of Pneumocystis carinii: implications for biological function and identification of potential drug targets.The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S154-5

Slaven, Bradley E; Porollo, Aleksey; Sesterhenn, Thomas; Smulian, A George; Cushion, Melanie T; Meller, Jaroslaw (2006. )Large-scale characterization of introns in the Pneumocystis carinii genome.The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S151-3

Joffrion, Tiffany M; Collins, Margaret S; Cushion, Melanie T (2006. )Microaerophilic conditions increase viability and affect responses of Pneumocystis carinii to drugs in vitro.The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S117-8

Kaneshiro, Edna S; Cali, Ann; Cushion, Melanie T; Lindsay, David S; Marciano-Cabral, Francine; Mead, Jan R; Weiss, Louis M (2006. )The International Workshops on Opportunistic Protists.The Journal of eukaryotic microbiology, ,53 Suppl 1 ,S1-7

Cushion,M.T.; Stringer,J.R. (2005. )Has the name really been changed? It has for most researchers .Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, ,41 (12 ),1756 -1758

Peterson,J.C.; Cushion,M.T. (2005. )Pneumocystis: not just pneumonia .Current opinion in microbiology, ,8 (4 ),393 -398

Keely,S.P.; Renauld,H.; Wakefield,A.E.; Cushion,M.T.; Smulian,A.G.; Fosker,N.; Fraser,A.; Harris,D.; Murphy,L.; Price,C.; Quail,M.A.; Seeger,K.; Sharp,S.; Tindal,C.J.; Warren,T.; Zuiderwijk,E.; Barrell,B.G.; Stringer,J.R.; Hall,N. (2005. )Gene arrays at Pneumocystis carinii telomeres .Genetics, ,170 (4 ),1589 -1600

Cushion, Melanie T; Stringer, James R (2005. )Has the name really been changed? It has for most researchers.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, ,41 (12 ),1756-8

Peterson, J Chad; Cushion, Melanie T (2005. )Pneumocystis: not just pneumonia.Current opinion in microbiology, ,8 (4 ),393-8

Keely, Scott P; Renauld, Hubert; Wakefield, Ann E; Cushion, Melanie T; Smulian, A George; Fosker, Nigel; Fraser, Audrey; Harris, David; Murphy, Lee; Price, Claire; Quail, Michael A; Seeger, Kathy; Sharp, Sarah; Tindal, Carolyn J; Warren, Tim; Zuiderwijk, Eduard; Barrell, Barclay G; Stringer, James R; Hall, Neil (2005. )Gene arrays at Pneumocystis carinii telomeres.Genetics, ,170 (4 ),1589-600

Cushion, Melanie T; Walzer, Peter D; Collins, Margaret S; Rebholz, Sandra; Vanden Eynde, Jean Jacques; Mayence, Annie; Huang, Tien L (2004. )Highly active anti-Pneumocystis carinii compounds in a library of novel piperazine-linked bisbenzamidines and related compounds.Antimicrobial agents and chemotherapy, ,48 (11 ),4209-16

Vanden Eynde, Jean Jacques; Mayence, Annie; Huang, Tien L; Collins, Margaret S; Rebholz, Sandra; Walzer, Peter D; Cushion, Melanie T (2004. )Novel bisbenzamidines as potential drug candidates for the treatment of Pneumocystis carinii pneumonia.Bioorganic & medicinal chemistry letters, ,14 (17 ),4545-8

Mayence, Annie; Vanden Eynde, Jean Jacques; Krogstad, Fran M; Krogstad, Donald J; Cushion, Melanie T; Huang, Tien L (2004. )Parallel solution-phase synthesis of conformationally restricted congeners of pentamidine and evaluation of their antiplasmodial activities.Journal of medicinal chemistry, ,47 (10 ),2700-5

Keely, Scott P; Fischer, Jared M; Cushion, Melanie T; Stringer, James R (2004. )Phylogenetic identification of Pneumocystis murina sp. nov., a new species in laboratory mice. Microbiology (Reading, England), ,150 (Pt 5 ),1153-65

Cushion, Melanie T (2004. )Pneumocystis: unraveling the cloak of obscurity.Trends in microbiology, ,12 (5 ),243-9

Cushion,M.T.; Walzer,P.D.; Collins,M.S.; Rebholz,S.; Vanden Eynde,J.J.; Mayence,A.; Huang,T.L. (2004. )Highly active anti-Pneumocystis carinii compounds in a library of novel piperazine-linked bisbenzamidines and related compounds .Antimicrobial Agents and Chemotherapy, ,48 (11 ),4209 -4216

Vanden Eynde,J.J.; Mayence,A.; Huang,T.L.; Collins,M.S.; Rebholz,S.; Walzer,P.D.; Cushion,M.T. (2004. )Novel bisbenzamidines as potential drug candidates for the treatment of Pneumocystis carinii pneumonia .Bioorganic & medicinal chemistry letters, ,14 (17 ),4545 -4548

Keely,S.P.; Fischer,J.M.; Cushion,M.T.; Stringer,J.R. (2004. )Phylogenetic identification of Pneumocystis murina sp. nov., a new species in laboratory mice .Microbiology (Reading, England), ,150 (Pt 5 ),1153 -1165

Cushion,M.T. (2004. )Pneumocystis: unraveling the cloak of obscurity .Trends in microbiology, ,12 (5 ),243 -249

Cushion,M.T. (2004. )Comparative genomics of Pneumocystis carinii with other protists: implications for life style .The Journal of eukaryotic microbiology, ,51 (1 ),30 -37

Sesterhenn,T.M.; Slaven,B.E.; Smulian,A.G.; Cushion,M.T. (2003. )Generation of sequencing libraries for the Pneumocystis Genome project .The Journal of eukaryotic microbiology, ,50 Suppl ,663 -665

Collins,M.S.; Bansil,S.; Cushion,M.T. (2003. )Expression profiling of the responses of Pneumocystis carinii to drug treatment using DNA macroarrays .The Journal of eukaryotic microbiology, ,50 Suppl ,605 -606

Linke,M.J.; Rebholz,S.; Collins,M.; Tanaka,R.; Cushion,M.T. (2003. )Noninvasive method for monitoring Pneumocystis carinii pneumonia .Emerging infectious diseases, ,9 (12 ),1613 -1616

Xu,Z.; Lance,B.; Vargas,C.; Arpinar,B.; Bhandarkar,S.; Kraemer,E.; Kochut,K.J.; Miller,J.A.; Wagner,J.R.; Weise,M.J.; Wunderlich,J.K.; Stringer,J.; Smulian,G.; Cushion,M.T.; Arnold,J. (2003. )Mapping by sequencing the Pneumocystis genome using the ordering DNA sequences V3 tool .Genetics, ,163 (4 ),1299 -1313

Linke, Michael J; Rebholz, Sandy; Collins, Margaret; Tanaka, Reiko; Cushion, Melanie T (2003. )Noninvasive method for monitoring Pneumocystis carinii pneumonia. Emerging infectious diseases, ,9 (12 ),1613-6

Xu, Zheng; Lance, Britton; Vargas, Claudia; Arpinar, Budak; Bhandarkar, Suchendra; Kraemer, Eileen; Kochut, Krys J; Miller, John A; Wagner, Jeff R; Weise, Michael J; Wunderlich, John K; Stringer, James; Smulian, George; Cushion, Melanie T; Arnold, Jonathan (2003. )Mapping by sequencing the Pneumocystis genome using the ordering DNA sequences V3 tool. Genetics, ,163 (4 ),1299-313

Keely, Scott P; Cushion, Melanie T; Stringer, James R (2003. )Diversity at the locus associated with transcription of a variable surface antigen of Pneumocystis carinii as an index of population structure and dynamics in infected rats. Infection and immunity, ,71 (1 ),47-60

Sesterhenn, Thomas M; Slaven, Bradley E; Smulian, A George; Cushion, Melanie T (2003. )Generation of sequencing libraries for the Pneumocystis Genome project. The Journal of eukaryotic microbiology, ,50 Suppl ,663-5

Collins, Margaret S; Bansil, Sandeep; Cushion, Melanie T (2003. )Expression profiling of the responses of Pneumocystis carinii to drug treatment using DNA macroarrays. The Journal of eukaryotic microbiology, ,50 Suppl ,605-6

Keely,S.P.; Cushion,M.T.; Stringer,J.R. (2003. )Diversity at the locus associated with transcription of a variable surface antigen of Pneumocystis carinii as an index of population structure and dynamics in infected rats .Infection and immunity, ,71 (1 ),47 -60

Icenhour,C.R.; Rebholz,S.L.; Collins,M.S.; Cushion,M.T. (2002. )Early acquisition of Pneumocystis carinii in neonatal rats as evidenced by PCR and oral swabs .Eukaryotic cell, ,1 (3 ),414 -419

Cornillot,E.; Keller,B.; Cushion,M.T.; Metenier,G.; Vivares,C.P. (2002. )Fine analysis of the Pneumocystis carinii f. sp. carinii genome by two-dimensional pulsed-field gel electrophoresis .Gene, ,293 (1-2 ),87 -95

Zhou, Wenxu; Nguyen, Thi Thuy Minh; Collins, Margaret S; Cushion, Melanie T; Nes, W David (2002. )Evidence for multiple sterol methyl transferase pathways in Pneumocystis carinii. Lipids, ,37 (12 ),1177-86

Cornillot, Emmanuel; Keller, Brian; Cushion, Melanie T; Méténier, Guy; Vivarès, Christian P (2002. )Fine analysis of the Pneumocystis carinii f. sp. carinii genome by two-dimensional pulsed-field gel electrophoresis. Gene, ,293 (1-2 ),87-95

Icenhour, Crystal R; Rebholz, Sandra L; Collins, Margaret S; Cushion, Melanie T (2002. )Early acquisition of Pneumocystis carinii in neonatal rats as evidenced by PCR and oral swabs. Eukaryotic cell, ,1 (3 ),414-9

Zhou,W.; Nguyen,T.T.; Collins,M.S.; Cushion,M.T.; Nes,W.D. (2002. )Evidence for multiple sterol methyl transferase pathways in Pneumocystis carinii .Lipids, ,37 (12 ),1177 -1186

Nahimana, A; Cushion, M T; Blanc, D S; Hauser, P M (2001. )Rapid PCR-single-strand conformation polymorphism method to differentiate and estimate relative abundance of Pneumocystis carinii special forms infecting rats.Journal of clinical microbiology, ,39 (12 ),4563-5

Walzer, P D; Ashbaugh, A; Collins, M; Cushion, M T (2001. )Anti-human immunodeficiency virus drugs are ineffective against Pneumocystis carinii in vitro and in vivo.The Journal of infectious diseases, ,184 (10 ),1355-7

Walzer, P D; Ashbaugh, A; Collins, M; Cushion, M T (2001. )In vitro and in vivo effects of quinupristin-dalfopristin against Pneumocystis carinii.Antimicrobial agents and chemotherapy, ,45 (11 ),3234-7

Icenhour, C R; Rebholz, S L; Collins, M S; Cushion, M T (2001. )Widespread occurrence of Pneumocystis carinii in commercial rat colonies detected using targeted PCR and oral swabs.Journal of clinical microbiology, ,39 (10 ),3437-41

Smulian, A G; Sesterhenn, T; Tanaka, R; Cushion, M T (2001. )The ste3 pheromone receptor gene of Pneumocystis carinii is surrounded by a cluster of signal transduction genes. Genetics, ,157 (3 ),991-1002

Cushion, M T; Orr, S; Keely, S P; Stringer, J R (2001. )Time between inoculations and karyotype forms of Pneumocystis carinii f. sp. Carinii influence outcome of experimental coinfections in rats.Infection and immunity, ,69 (1 ),97-107

Stringer, J R; Cushion, M T; Wakefield, A E (2001. )New nomenclature for the genus Pneumocystis. The Journal of eukaryotic microbiology, ,Suppl ,184S-189S

Cushion, M T; Smulian, A G (2001. )The pneumocystis genome project: update and issues. The Journal of eukaryotic microbiology, ,Suppl ,182S-183S

Collins, M S; Cushion, M T (2001. )Standardization of an in vitro drug screening assay by use of cryopreserved and characterized Pneumocystis carinii populations. The Journal of eukaryotic microbiology, ,Suppl ,178S-179S

Icenhour, C R; Cushion, M T (2001. )Putative transmissive form of Pneumocystis carinii f. sp. carinii. The Journal of eukaryotic microbiology, ,Suppl ,139S-140S

Icenhour, C R; Rebholz, S L; Collins, M S; Cushion, M T (2001. )Early acquisition of Pneumocystis carinii in neonatal rats using targeted PCR and oral swabs. The Journal of eukaryotic microbiology, ,Suppl ,135S-136S

Keely, S P; Wakefield, A E; Cushion, M T; Smulian, A G; Hall, N; Barrell, B G; Stringer, J R (2001. )Detailed structure of Pneumocystis carinii chromosome ends. The Journal of eukaryotic microbiology, ,Suppl ,118S-120S

Rebholz, S L; Cushion, M T (2001. )Three new karyotype forms of Pneumocystis carinii f. sp. carinii identified by contoured clamped homogeneous electrical field (CHEF) electrophoresis. The Journal of eukaryotic microbiology, ,Suppl ,109S-110S

Cushion, M T; Beck, J M (2001. )Summary of Pneumocystis research presented at the 7th International Workshop on Opportunistic Protists. The Journal of eukaryotic microbiology, ,Suppl ,101S-105S

Stringer,J.R.; Cushion,M.T.; Wakefield,A.E. (2001. )New nomenclature for the genus Pneumocystis .The Journal of eukaryotic microbiology, ,Suppl ,184S -189S

Cushion,M.T.; Smulian,A.G. (2001. )The pneumocystis genome project: update and issues .The Journal of eukaryotic microbiology, ,Suppl ,182S -183S

Collins,M.S.; Cushion,M.T. (2001. )Standardization of an in vitro drug screening assay by use of cryopreserved and characterized Pneumocystis carinii populations .The Journal of eukaryotic microbiology, ,Suppl ,178S -179S

Icenhour,C.R.; Cushion,M.T. (2001. )Putative transmissive form of Pneumocystis carinii f. sp. carinii .The Journal of eukaryotic microbiology, ,Suppl ,139S -140S

Icenhour,C.R.; Rebholz,S.L.; Collins,M.S.; Cushion,M.T. (2001. )Early acquisition of Pneumocystis carinii in neonatal rats using targeted PCR and oral swabs .The Journal of eukaryotic microbiology, ,Suppl ,135S -136S

Keely,S.P.; Wakefield,A.E.; Cushion,M.T.; Smulian,A.G.; Hall,N.; Barrell,B.G.; Stringer,J.R. (2001. )Detailed structure of Pneumocystis carinii chromosome ends .The Journal of eukaryotic microbiology, ,Suppl ,118S -120S

Rebholz,S.L.; Cushion,M.T. (2001. )Three new karyotype forms of Pneumocystis carinii f. sp. carinii identified by contoured clamped homogeneous electrical field (CHEF) electrophoresis .The Journal of eukaryotic microbiology, ,Suppl ,109S -110S

Cushion,M.T.; Beck,J.M. (2001. )Summary of Pneumocystis research presented at the 7th International Workshop on Opportunistic Protists .The Journal of eukaryotic microbiology, ,Suppl ,101S -105S

Nahimana,A.; Cushion,M.T.; Blanc,D.S.; Hauser,P.M. (2001. )Rapid PCR-single-strand conformation polymorphism method to differentiate and estimate relative abundance of Pneumocystis carinii special forms infecting rats .Journal of clinical microbiology, ,39 (12 ),4563 -4565

Walzer,P.D.; Ashbaugh,A.; Collins,M.; Cushion,M.T. (2001. )Anti-human immunodeficiency virus drugs are ineffective against Pneumocystis carinii in vitro and in vivo .The Journal of infectious diseases, ,184 (10 ),1355 -1357

Walzer,P.D.; Ashbaugh,A.; Collins,M.; Cushion,M.T. (2001. )In vitro and in vivo effects of quinupristin-dalfopristin against Pneumocystis carinii .Antimicrobial Agents and Chemotherapy, ,45 (11 ),3234 -3237

Icenhour,C.R.; Rebholz,S.L.; Collins,M.S.; Cushion,M.T. (2001. )Widespread occurrence of Pneumocystis carinii in commercial rat colonies detected using targeted PCR and oral swabs .Journal of clinical microbiology, ,39 (10 ),3437 -3441

Smulian,A.G.; Sesterhenn,T.; Tanaka,R.; Cushion,M.T. (2001. )The ste3 pheromone receptor gene of Pneumocystis carinii is surrounded by a cluster of signal transduction genes .Genetics, ,157 (3 ),991 -1002

Cushion,M.T.; Orr,S.; Keely,S.P.; Stringer,J.R. (2001. )Time between inoculations and karyotype forms of Pneumocystis carinii f. sp. Carinii influence outcome of experimental coinfections in rats .Infection and immunity, ,69 (1 ),97 -107

Kaneshiro,E.S.; Collins,M.S.; Cushion,M.T. (2000. )Inhibitors of sterol biosynthesis and amphotericin B reduce the viability of pneumocystis carinii f. sp. carinii .Antimicrobial Agents and Chemotherapy, ,44 (6 ),1630 -1638

Cushion,M.T.; Collins,M.; Hazra,B.; Kaneshiro,E.S. (2000. )Effects of atovaquone and diospyrin-based drugs on the cellular ATP of Pneumocystis carinii f. sp. carinii .Antimicrobial Agents and Chemotherapy, ,44 (3 ),713 -719

Kaneshiro, E S; Collins, M S; Cushion, M T (2000. )Inhibitors of sterol biosynthesis and amphotericin B reduce the viability of pneumocystis carinii f. sp. carinii. Antimicrobial agents and chemotherapy, ,44 (6 ),1630-8

Cushion, M T; Collins, M; Hazra, B; Kaneshiro, E S (2000. )Effects of atovaquone and diospyrin-based drugs on the cellular ATP of Pneumocystis carinii f. sp. carinii. Antimicrobial agents and chemotherapy, ,44 (3 ),713-9

Kaneshiro,E.S.; Collins,M.; Cushion,M.T. (1999. )Effects of sterol inhibitors on the ATP content of Pneumocystis carinii .The Journal of eukaryotic microbiology, ,46 (5 ),142S -143S

Keely,S.P.; Cushion,M.T.; Stringer,J.R. (1999. )Determination of the maximum frequency of genetic rearrangements associated with Pneumocystis carinii surface antigen variation .The Journal of eukaryotic microbiology, ,46 (5 ),128S

Cushion,M.T.; Linke,M.J.; Collins,M.; Keely,S.P.; Stringer,J.R. (1999. )The minimum number of Pneumocystis carinii f. sp. carinii organisms required to establish infections is very low .The Journal of eukaryotic microbiology, ,46 (5 ),111S

Icenhour,C.R.; Arnold,J.; Cushion,M.T. (1999. )Interactions of two Pneumocystis carinii populations within rat lungs .The Journal of eukaryotic microbiology, ,46 (5 ),107S -108S

Cushion,M.T. (1999. )Summary of the Pneumocystis research presented at the 6th International Workshop on Opportunistic Protists .The Journal of eukaryotic microbiology, ,46 (5 ),85S -88S

Palmer,R.J.; Cushion,M.T.; Wakefield,A.E. (1999. )Discrimination of rat-derived Pneumocystis carinii f. sp. Carinii and Pneumocystis carinii f. sp. Ratti using the polymerase chain reaction .Molecular and cellular probes, ,13 (2 ),147 -155

Weisbroth,S.H.; Geistfeld,J.; Weisbroth,S.P.; Williams,B.; Feldman,S.H.; Linke,M.J.; Orr,S.; Cushion,M.T. (1999. )Latent Pneumocystis carinii infection in commercial rat colonies: comparison of inductive immunosuppressants plus histopathology, PCR, and serology as detection methods .Journal of clinical microbiology, ,37 (5 ),1441 -1446

Weisbroth, S H; Geistfeld, J; Weisbroth, S P; Williams, B; Feldman, S H; Linke, M J; Orr, S; Cushion, M T (1999. )Latent Pneumocystis carinii infection in commercial rat colonies: comparison of inductive immunosuppressants plus histopathology, PCR, and serology as detection methods. Journal of clinical microbiology, ,37 (5 ),1441-6

Palmer, R J; Cushion, M T; Wakefield, A E (1999. )Discrimination of rat-derived Pneumocystis carinii f. sp. Carinii and Pneumocystis carinii f. sp. Ratti using the polymerase chain reaction.Molecular and cellular probes, ,13 (2 ),147-55

Cushion, M T (1998. )Taxonomy, genetic organization, and life cycle of Pneumocystis carinii. Seminars in respiratory infections, ,13 (4 ),304-12

Cushion, M T (1998. )Genetic heterogeneity of rat-derived Pneumocystis. FEMS immunology and medical microbiology, ,22 (1-2 ),51-8

Stringer, J R; Cushion, M T (1998. )The genome of Pneumocystis carinii. FEMS immunology and medical microbiology, ,22 (1-2 ),15-26

Cushion,M.T. (1998. )Taxonomy, genetic organization, and life cycle of Pneumocystis carinii .Seminars in respiratory infections, ,13 (4 ),304 -312

Cushion,M.T. (1998. )Genetic heterogeneity of rat-derived Pneumocystis .FEMS immunology and medical microbiology, ,22 (1-2 ),51 -58

Stringer,J.R.; Cushion,M.T. (1998. )The genome of Pneumocystis carinii .FEMS immunology and medical microbiology, ,22 (1-2 ),15 -26

Cushion,M.T.; Orr,S.; Arnold,J. (1997. )Interactions between 2 Pneumocystis populations within the same host .The Journal of eukaryotic microbiology, ,44 (6 ),9S

Arnold,J.; Cushion,M.T. (1997. )Constructing a physical map of the Pneumocystis genome .The Journal of eukaryotic microbiology, ,44 (6 ),8S

Cushion,M.T.; Arnold,J. (1997. )Proposal for a Pneumocystis genome project .The Journal of eukaryotic microbiology, ,44 (6 ),7S

Stringer,J.R.; Wakefield,A.E.; Cushion,M.T.; Dei-Cas,E. (1997. )Pneumocystis taxonomy and nomenclature: an update .The Journal of eukaryotic microbiology, ,44 (6 ),5S -6S

Cushion,M.T.; Walzer,P.D.; Smulian,A.G.; Linke,M.J.; Ruffolo,J.J.; Kaneshiro,E.S.; Stringer,J.R. (1997. )Terminology for the life cycle of Pneumocystis carinii .Infection and immunity, ,65 (10 ),4365; author reply 4366

Cushion,M.T.; Chen,F.; Kloepfer,N. (1997. )A cytotoxicity assay for evaluation of candidate anti-Pneumocystis carinii agents .Antimicrobial Agents and Chemotherapy, ,41 (2 ),379 -384

Beach,D.H.; Chen,F.; Cushion,M.T.; Macomber,R.S.; Krudy,G.A.; Wyder,M.A.; Kaneshiro,E.S. (1997. )Effects of steroidal allenic phosphonic acid derivatives on the parasitic protists Leishmania donovani, Leishmania mexicana mexicana, and Pneumocystis carinii carinii .Antimicrobial Agents and Chemotherapy, ,41 (1 ),162 -168

Cushion, M T; Walzer, P D; Smulian, A G; Linke, M J; Ruffolo, J J; Kaneshiro, E S; Stringer, J R (1997. )Terminology for the life cycle of Pneumocystis carinii. Infection and immunity, ,65 (10 ),4365; author reply 4

Perlada, D E; Smulian, A G; Cushion, M T (1997. )Molecular epidemiology and antibiotic susceptibility of enterococci in Cincinnati, Ohio: a prospective citywide survey. Journal of clinical microbiology, ,35 (9 ),2342-7

Cushion, M T; Chen, F; Kloepfer, N (1997. )A cytotoxicity assay for evaluation of candidate anti-Pneumocystis carinii agents. Antimicrobial agents and chemotherapy, ,41 (2 ),379-84

Beach, D H; Chen, F; Cushion, M T; Macomber, R S; Krudy, G A; Wyder, M A; Kaneshiro, E S (1997. )Effects of steroidal allenic phosphonic acid derivatives on the parasitic protists Leishmania donovani, Leishmania mexicana mexicana, and Pneumocystis carinii carinii. Antimicrobial agents and chemotherapy, ,41 (1 ),162-8

Vasquez, J; Smulian, A G; Linke, M J; Cushion, M T (1996. )Antigenic differences associated with genetically distinct Pneumocystis carinii from rats. Infection and immunity, ,64 (1 ),290-7

Cushion,M.T.; Walzer,P.D. (1996. )Closing comments: a retrospective perspective 4th International Workshop on Pneumocystis .The Journal of eukaryotic microbiology, ,43 (5 ),60S -61S

Keely,S.P.; Cushion,M.T.; Stringer,J.R. (1996. )Stability of four genetic loci in Pneumocystis carinii sp. f. carinii .The Journal of eukaryotic microbiology, ,43 (5 ),49S

Cushion,M.T.; Orr,S. (1996. )Kinetics of 2 genetically distinct Pneumocystis carinii populations in rat colonies .The Journal of eukaryotic microbiology, ,43 (5 ),46S

Vasquez,J.; Smulian,A.G.; Linke,M.J.; Cushion,M.T. (1996. )Antigenic differences associated with genetically distinct Pneumocystis carinii from rats .Infection and immunity, ,64 (1 ),290 -297

O'Leary,T.J.; Tsai,M.M.; Wright,C.F.; Cushion,M.T. (1995. )Use of semiquantitative PCR to assess onset and treatment of Pneumocystis carinii infection in rat model .Journal of clinical microbiology, ,33 (3 ),718 -724

O'Leary, T J; Tsai, M M; Wright, C F; Cushion, M T (1995. )Use of semiquantitative PCR to assess onset and treatment of Pneumocystis carinii infection in rat model. Journal of clinical microbiology, ,33 (3 ),718-24

Smulian, A G; Linke, M J; Cushion, M T; Baughman, R P; Frame, P T; Dohn, M N; White, M L; Walzer, P D (1994. )Analysis of Pneumocystis carinii organism burden, viability and antigens in bronchoalveolar lavage fluid in AIDS patients with pneumocystosis: correlation with disease severity. AIDS (London, England), ,8 (11 ),1555-62

Chen, F; Cushion, M T (1994. )Use of an ATP bioluminescent assay to evaluate viability of Pneumocystis carinii from rats. Journal of clinical microbiology, ,32 (11 ),2791-800

Cushion, M T; Harmsen, A; Matsumoto, Y; Stringer, J R; Wakefield, A E; Yamada, M (1994. )Recent advances in the biology of Pneumocystis carinii. Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, ,32 Suppl 1 ,217-28

De Stefano, J A; Foy, J M; Sullivan, D W; Dawes, S M; Cushion, M T; Babcock, G F; Sleight, R G; van Halbeek, H; Walzer, P D (1994. )Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations. Parasitology research, ,80 (1 ),1-9

Chen,F.; Cushion,M.T. (1994. )Use of an ATP bioluminescent assay to evaluate viability of Pneumocystis carinii from rats .Journal of clinical microbiology, ,32 (11 ),2791 -2800

Smulian,A.G.; Linke,M.J.; Cushion,M.T.; Baughman,R.P.; Frame,P.T.; Dohn,M.N.; White,M.L.; Walzer,P.D. (1994. )Analysis of Pneumocystis carinii organism burden, viability and antigens in bronchoalveolar lavage fluid in AIDS patients with pneumocystosis: correlation with disease severity .AIDS (London, England), ,8 (11 ),1555 -1562

Langreth,S.G.; Kim,G.M.; Cushion,M.T. (1994. )Evaluation of procedures used to isolate Pneumocystis carinii from infected rat lungs .The Journal of eukaryotic microbiology, ,41 (5 ),95S -96S

Chen,F.; Cushion,M.T. (1994. )Use of fluorescent probes to investigate the metabolic state of Pneumocystis carinii mitochondria .The Journal of eukaryotic microbiology, ,41 (5 ),79S

Andrews,R.P.; Theus,S.A.; Cushion,M.T.; Walzer,P.D. (1994. )Comparison of the antigenic recognition of the major surface glycoprotein of Pneumocystis carinii isolated from different rat strains .The Journal of eukaryotic microbiology, ,41 (5 ),72S

Weinberg,G.A.; O'Gara,M.J.; Cushion,M.T. (1994. )Coinfection of rats with genetically diverse forms of Pneumocystis carinii demonstrated by P. carinii inosine monophosphate dehydrogenase gene polymorphism .The Journal of eukaryotic microbiology, ,41 (5 ),119S

Weinberg,G.A.; Dykstra,C.C.; Durant,P.J.; Cushion,M.T. (1994. )Chromosomal localization of 20 genes to five distinct pulsed field gel karyotypic forms of rat Pneumocystis carinii .The Journal of eukaryotic microbiology, ,41 (5 ),117S

Vasquez,J.; Smulian,A.G.; Linke,M.J.; Cushion,M.T. (1994. )Antigenic differences among genetically distinct types of rat-derived Pneumocystis carinii .The Journal of eukaryotic microbiology, ,41 (5 ),114S

De Stefano,J.A.; Foy,J.M.; Sullivan,D.W.; Dawes,S.M.; Cushion,M.T.; Babcock,G.F.; Sleight,R.G.; van Halbeek,H.; Walzer,P.D. (1994. )Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations .Parasitology research, ,80 (1 ),1 -9

Settnes,O.P.; Nielsen,P.B.; Bucala,R.; Linke,M.J.; Cushion,M.T. (1994. )A survey of birds in Denmark for the presence of Pneumocystis carinii .Avian Diseases, ,38 (1 ),1 -10

Cushion,M.T.; Harmsen,A.; Matsumoto,Y.; Stringer,J.R.; Wakefield,A.E.; Yamada,M. (1994. )Recent advances in the biology of Pneumocystis carinii .Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, ,32 Suppl 1 ,217 -228

Cushion,M.T.; Kaselis,M.; Stringer,S.L.; Stringer,J.R. (1993. )Genetic stability and diversity of Pneumocystis carinii infecting rat colonies .Infection and immunity, ,61 (11 ),4801 -4813

Stringer,J.R.; Stringer,S.L.; Zhang,J.; Baughman,R.; Smulian,A.G.; Cushion,M.T. (1993. )Molecular genetic distinction of Pneumocystis carinii from rats and humans .The Journal of eukaryotic microbiology, ,40 (6 ),733 -741

Cushion,M.T.; Zhang,J.; Kaselis,M.; Giuntoli,D.; Stringer,S.L.; Stringer,J.R. (1993. )Evidence for two genetic variants of Pneumocystis carinii coinfecting laboratory rats .Journal of clinical microbiology, ,31 (5 ),1217 -1223

Zhang,J.; Cushion,M.T.; Stringer,J.R. (1993. )Molecular characterization of a novel repetitive element from Pneumocystis carinii from rats .Journal of clinical microbiology, ,31 (2 ),244 -248

Cushion, M T; Kaselis, M; Stringer, S L; Stringer, J R (1993. )Genetic stability and diversity of Pneumocystis carinii infecting rat colonies. Infection and immunity, ,61 (11 ),4801-13

Cushion, M T; Zhang, J; Kaselis, M; Giuntoli, D; Stringer, S L; Stringer, J R (1993. )Evidence for two genetic variants of Pneumocystis carinii coinfecting laboratory rats. Journal of clinical microbiology, ,31 (5 ),1217-23

Zhang, J; Cushion, M T; Stringer, J R (1993. )Molecular characterization of a novel repetitive element from Pneumocystis carinii from rats. Journal of clinical microbiology, ,31 (2 ),244-8

De Stefano, J A; Trinkle, L S; Walzer, P D; Cushion, M T (1992. )Flow cytometric analyses of lectin binding to Pneumocystis carinii surface carbohydrates. The Journal of parasitology, ,78 (2 ),271-80

Stringer, J R; Edman, J C; Cushion, M T; Richards, F F; Watanabe, J (1992. )The fungal nature of Pneumocystis. Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, ,30 Suppl 1 ,271-8

De Stefano,J.A.; Trinkle,L.S.; Walzer,P.D.; Cushion,M.T. (1992. )Flow cytometric analyses of lectin binding to Pneumocystis carinii surface carbohydrates .The Journal of parasitology, ,78 (2 ),271 -280

Stringer,J.R.; Edman,J.C.; Cushion,M.T.; Richards,F.F.; Watanabe,J. (1992. )The fungal nature of Pneumocystis .Journal of medical and veterinary mycology : bi-monthly publication of the International Society for Human and Animal Mycology, ,30 Suppl 1 ,271 -278

Zhang,J.X.; Cushion,M.T.; Giuntoli,D.; Hong,S.T.; Meade,J.C.; Smulian,A.G.; Stringer,S.L.; Stringer,J.J. (1991. )Analysis of the Pneumocystis carinii genome .The Journal of protozoology, ,38 (6 ),69S

Cushion,M.T.; Linke,M.J. (1991. )Factors influencing Pneumocystis infection in the immunocompromised rat .The Journal of protozoology, ,38 (6 ),133S -135S

Kaneshiro,E.S.; Wu,Y.P.; Cushion,M.T. (1991. )Assays for testing Pneumocystis carinii viability .The Journal of protozoology, ,38 (6 ),85S -87S

Cushion,M.T.; Stringer,J.R.; Walzer,P.D. (1991. )Cellular and molecular biology of Pneumocystis carinii .International review of cytology, ,131 ,59 -107

Cushion, M T; Stringer, J R; Walzer, P D (1991. )Cellular and molecular biology of Pneumocystis carinii. International review of cytology, ,131 ,59-107

Hong, S T; Steele, P E; Cushion, M T; Walzer, P D; Stringer, S L; Stringer, J R (1990. )Pneumocystis carinii karyotypes. Journal of clinical microbiology, ,28 (8 ),1785-95

Cushion, M T; Ebbets, D (1990. )Growth and metabolism of Pneumocystis carinii in axenic culture. Journal of clinical microbiology, ,28 (6 ),1385-94

Cushion, M T; Hong, S T; Steele, P E; Stringer, S L; Walzer, P D; Stringer, J R (1990. )Molecular biology of Pneumocystis carinii. Annals of the New York Academy of Sciences, ,616 ,415-20

De Stefano,J.A.; Cushion,M.T.; Puvanesarajah,V.; Walzer,P.D. (1990. )Analysis of Pneumocystis carinii cyst wall. II. Sugar composition .The Journal of protozoology, ,37 (5 ),436 -441

De Stefano,J.A.; Cushion,M.T.; Sleight,R.G.; Walzer,P.D. (1990. )Analysis of Pneumocystis carinii cyst wall. I. Evidence for an outer surface membrane .The Journal of protozoology, ,37 (5 ),428 -435

Hong,S.T.; Steele,P.E.; Cushion,M.T.; Walzer,P.D.; Stringer,S.L.; Stringer,J.R. (1990. )Pneumocystis carinii karyotypes .Journal of clinical microbiology, ,28 (8 ),1785 -1795

Cushion,M.T.; Ebbets,D. (1990. )Growth and metabolism of Pneumocystis carinii in axenic culture .Journal of clinical microbiology, ,28 (6 ),1385 -1394

Cushion,M.T.; Hong,S.T.; Steele,P.E.; Stringer,S.L.; Walzer,P.D.; Stringer,J.R. (1990. )Molecular biology of Pneumocystis carinii .Annals of the New York Academy of Sciences, ,616 ,415 -420

Linke,M.J.; Cushion,M.T.; Walzer,P.D. (1989. )Properties of the major antigens of rat and human Pneumocystis carinii .Infection and immunity, ,57 (5 ),1547 -1555

Stringer,S.L.; Stringer,J.R.; Blase,M.A.; Walzer,P.D.; Cushion,M.T. (1989. )Pneumocystis carinii: sequence from ribosomal RNA implies a close relationship with fungi .Experimental parasitology, ,68 (4 ),450 -461

Kaneshiro,E.S.; Cushion,M.T.; Walzer,P.D.; Jayasimhulu,K. (1989. )Analyses of Pneumocystis fatty acids .The Journal of protozoology, ,36 (1 ),69S -72S

De Stefano,J.A.; Cushion,M.T.; Trinkle,L.S.; Walzer,P.D. (1989. )Lectins as probes to Pneumocystis carinii surface glycocomplexes .The Journal of protozoology, ,36 (1 ),65S -66S

Ruffolo,J.J.; Cushion,M.T.; Walzer,P.D. (1989. )Ultrastructural observations on life cycle stages of Pneumocystis carinii .The Journal of protozoology, ,36 (1 ),53S -54S

Stringer,S.L.; Hudson,K.; Blase,M.A.; Walzer,P.D.; Cushion,M.T.; Stringer,J.R. (1989. )Sequence from ribosomal RNA of Pneumocystis carinii compared to those of four fungi suggests an ascomycetous affinity .The Journal of protozoology, ,36 (1 ),14S -16S

Walzer,P.D.; Cushion,M.T. (1989. )Immunobiology of Pneumocystis carinii .Pathology and immunopathology research, ,8 (3-4 ),127 -140

Cushion,M.T. (1989. )In vitro studies of Pneumocystis carinii .The Journal of protozoology, ,36 (1 ),45 -52

Cushion,M.T.; Blase,M.A.; Walzer,P.D. (1989. )A method for isolation of RNA from Pneumocystis carinii .The Journal of protozoology, ,36 (1 ),12S -14S

Stringer, S L; Stringer, J R; Blase, M A; Walzer, P D; Cushion, M T (1989. )Pneumocystis carinii: sequence from ribosomal RNA implies a close relationship with fungi. Experimental parasitology, ,68 (4 ),450-61

Linke, M J; Cushion, M T; Walzer, P D (1989. )Properties of the major antigens of rat and human Pneumocystis carinii. Infection and immunity, ,57 (5 ),1547-55

Walzer, P D; Cushion, M T (1989. )Immunobiology of Pneumocystis carinii. Pathology and immunopathology research, ,8 (3-4 ),127-40

Cushion, M T; DeStefano, J A; Walzer, P D (1988. )Pneumocystis carinii: surface reactive carbohydrates detected by lectin probes. Experimental parasitology, ,67 (2 ),137-47

Walzer, P D; Kim, C K; Foy, J; Linke, M J; Cushion, M T (1988. )Cationic antitrypanosomal and other antimicrobial agents in the therapy of experimental Pneumocystis carinii pneumonia. Antimicrobial agents and chemotherapy, ,32 (6 ),896-905

Cushion, M T; Ruffolo, J J; Walzer, P D (1988. )Analysis of the developmental stages of Pneumocystis carinii, in vitro. Laboratory investigation; a journal of technical methods and pathology, ,58 (3 ),324-31

Walzer, P D; Kim, C K; Foy, J M; Linke, M J; Cushion, M T (1988. )Inhibitors of folic acid synthesis in the treatment of experimental Pneumocystis carinii pneumonia. Antimicrobial agents and chemotherapy, ,32 (1 ),96-103

Cushion,M.T.; DeStefano,J.A.; Walzer,P.D. (1988. )Pneumocystis carinii: surface reactive carbohydrates detected by lectin probes .Experimental parasitology, ,67 (2 ),137 -147

Walzer,P.D.; Kim,C.K.; Foy,J.; Linke,M.J.; Cushion,M.T. (1988. )Cationic antitrypanosomal and other antimicrobial agents in the therapy of experimental Pneumocystis carinii pneumonia .Antimicrobial Agents and Chemotherapy, ,32 (6 ),896 -905

Cushion,M.T.; Ruffolo,J.J.; Walzer,P.D. (1988. )Analysis of the developmental stages of Pneumocystis carinii, in vitro .Laboratory investigation; a journal of technical methods and pathology, ,58 (3 ),324 -331

Walzer,P.D.; Kim,C.K.; Foy,J.M.; Linke,M.J.; Cushion,M.T. (1988. )Inhibitors of folic acid synthesis in the treatment of experimental Pneumocystis carinii pneumonia .Antimicrobial Agents and Chemotherapy, ,32 (1 ),96 -103

Walzer,P.D.; Stanforth,D.; Linke,M.J.; Cushion,M.T. (1987. )Pneumocystis carinii: immunoblotting and immunofluorescent analyses of serum antibodies during experimental rat infection and recovery .Experimental parasitology, ,63 (3 ),319 -328

Walzer,P.D.; Cushion,M.T.; Juranek,D.; Walls,K.; Armstrong,D.; Gold,J.; Young,L.S.; Pesanti,E.; Graves,D.C.; Ivey,M.B. (1987. )Serology and P carinii .Chest, ,91 (6 ),935 -936

Kim,C.K.; Foy,J.M.; Cushion,M.T.; Stanforth,D.; Linke,M.J.; Hendrix,H.L.; Walzer,P.D. (1987. )Comparison of histologic and quantitative techniques in evaluation of therapy for experimental Pneumocystis carinii pneumonia .Antimicrobial Agents and Chemotherapy, ,31 (2 ),197 -201

Walzer, P D; Cushion, M T; Juranek, D; Walls, K; Armstrong, D; Gold, J; Young, L S; Pesanti, E; Graves, D C; Ivey, M B (1987. )Serology and P carinii. Chest, ,91 (6 ),935-6

Walzer, P D; Stanforth, D; Linke, M J; Cushion, M T (1987. )Pneumocystis carinii: immunoblotting and immunofluorescent analyses of serum antibodies during experimental rat infection and recovery. Experimental parasitology, ,63 (3 ),319-28

Kim, C K; Foy, J M; Cushion, M T; Stanforth, D; Linke, M J; Hendrix, H L; Walzer, P D (1987. )Comparison of histologic and quantitative techniques in evaluation of therapy for experimental Pneumocystis carinii pneumonia. Antimicrobial agents and chemotherapy, ,31 (2 ),197-201

Ruffolo, J J; Cushion, M T; Walzer, P D (1986. )Techniques for examining Pneumocystis carinii in fresh specimens. Journal of clinical microbiology, ,23 (1 ),17-21

Ruffolo,J.J.; Cushion,M.T.; Walzer,P.D. (1986. )Techniques for examining Pneumocystis carinii in fresh specimens .Journal of clinical microbiology, ,23 (1 ),17 -21

Cushion,M.T.; Stanforth,D.; Linke,M.J.; Walzer,P.D. (1985. )Method of testing the susceptibility of Pneumocystis carinii to antimicrobial agents in vitro .Antimicrobial Agents and Chemotherapy, ,28 (6 ),796 -801

Cushion,M.T.; Ruffolo,J.J.; Linke,M.J.; Walzer,P.D. (1985. )Pneumocystis carinii: growth variables and estimates in the A549 and WI-38 VA13 human cell lines .Experimental parasitology, ,60 (1 ),43 -54

Cushion, M T; Stanforth, D; Linke, M J; Walzer, P D (1985. )Method of testing the susceptibility of Pneumocystis carinii to antimicrobial agents in vitro. Antimicrobial agents and chemotherapy, ,28 (6 ),796-801

Cushion, M T; Ruffolo, J J; Linke, M J; Walzer, P D (1985. )Pneumocystis carinii: growth variables and estimates in the A549 and WI-38 VA13 human cell lines. Experimental parasitology, ,60 (1 ),43-54

Walzer, P D; LaBine, M; Redington, T J; Cushion, M T (1984. )Predisposing factors in Pneumocystis carinii pneumonia: effects of tetracycline, protein malnutrition, and corticosteroids on hosts. Infection and immunity, ,46 (3 ),747-53

Walzer, P D; LaBine, M; Redington, T J; Cushion, M T (1984. )Lymphocyte changes during chronic administration of and withdrawal from corticosteroids: relation to Pneumocystis carinii pneumonia. Journal of immunology (Baltimore, Md. : 1950), ,133 (5 ),2502-8

Cushion, M T; Walzer, P D (1984. )Growth and serial passage of Pneumocystis carinii in the A549 cell line. Infection and immunity, ,44 (2 ),245-51

Cushion, M T; Walzer, P D (1984. )Cultivation of Pneumocystis carinii in lung-derived cell lines. The Journal of infectious diseases, ,149 (4 ),644

Walzer,P.D.; LaBine,M.; Redington,T.J.; Cushion,M.T. (1984. )Predisposing factors in Pneumocystis carinii pneumonia: effects of tetracycline, protein malnutrition, and corticosteroids on hosts .Infection and immunity, ,46 (3 ),747 -753

Walzer,P.D.; LaBine,M.; Redington,T.J.; Cushion,M.T. (1984. )Lymphocyte changes during chronic administration of and withdrawal from corticosteroids: relation to Pneumocystis carinii pneumonia .Journal of immunology (Baltimore, Md.: 1950), ,133 (5 ),2502 -2508

Cushion,M.T.; Walzer,P.D. (1984. )Growth and serial passage of Pneumocystis carinii in the A549 cell line .Infection and immunity, ,44 (2 ),245 -251

Cushion,M.T.; Walzer,P.D. (1984. )Cultivation of Pneumocystis carinii in lung-derived cell lines .The Journal of infectious diseases, ,149 (4 ),644

Kaneshiro, Edna S; Cushion, Melanie T; Marciano-Cabral, Francine; Weiss, Louis M; Xiao, Lihua Highlights and summaries of the 11th International Workshops on Opportunistic Protists.The Journal of eukaryotic microbiology, ,58 (1 ),1-6

Kaneshiro, E S; Collins, M; Cushion, M T Effects of sterol inhibitors on the ATP content of Pneumocystis carinii. The Journal of eukaryotic microbiology, ,46 (5 ),142S-143S

Keely, S P; Cushion, M T; Stringer, J R Determination of the maximum frequency of genetic rearrangements associated with Pneumocystis carinii surface antigen variation. The Journal of eukaryotic microbiology, ,46 (5 ),128S

Cushion, M T; Linke, M J; Collins, M; Keely, S P; Stringer, J R The minimum number of Pneumocystis carinii f. sp. carinii organisms required to establish infections is very low. The Journal of eukaryotic microbiology, ,46 (5 ),111S

Icenhour, C R; Arnold, J; Cushion, M T Interactions of two Pneumocystis carinii populations within rat lungs. The Journal of eukaryotic microbiology, ,46 (5 ),107S-108S

Cushion, M T Summary of the Pneumocystis research presented at the 6th International Workshop on Opportunistic Protists. The Journal of eukaryotic microbiology, ,46 (5 ),85S-88S

Redhead, Scott A; Cushion, Melanie T; Frenkel, Jacob K; Stringer, James R Pneumocystis and Trypanosoma cruzi: nomenclature and typifications.The Journal of eukaryotic microbiology, ,53 (1 ),2-11

Kaneshiro, E S; Cushion, M T; Walzer, P D; Jayasimhulu, K Analyses of Pneumocystis fatty acids. The Journal of protozoology, ,36 (1 ),69S-72S

De Stefano, J A; Cushion, M T; Trinkle, L S; Walzer, P D Lectins as probes to Pneumocystis carinii surface glycocomplexes. The Journal of protozoology, ,36 (1 ),65S-66S

Ruffolo, J J; Cushion, M T; Walzer, P D Ultrastructural observations on life cycle stages of Pneumocystis carinii. The Journal of protozoology, ,36 (1 ),53S-54S

Stringer, S L; Hudson, K; Blase, M A; Walzer, P D; Cushion, M T; Stringer, J R Sequence from ribosomal RNA of Pneumocystis carinii compared to those of four fungi suggests an ascomycetous affinity. The Journal of protozoology, ,36 (1 ),14S-16S

Cushion, M T In vitro studies of Pneumocystis carinii. The Journal of protozoology, ,36 (1 ),45-52

Cushion, M T; Blase, M A; Walzer, P D A method for isolation of RNA from Pneumocystis carinii. The Journal of protozoology, ,36 (1 ),12S-14S

De Stefano, J A; Cushion, M T; Puvanesarajah, V; Walzer, P D Analysis of Pneumocystis carinii cyst wall. II. Sugar composition. The Journal of protozoology, ,37 (5 ),436-41

De Stefano, J A; Cushion, M T; Sleight, R G; Walzer, P D Analysis of Pneumocystis carinii cyst wall. I. Evidence for an outer surface membrane. The Journal of protozoology, ,37 (5 ),428-35

Cushion, Melanie T Comparative genomics of Pneumocystis carinii with other protists: implications for life style. The Journal of eukaryotic microbiology, ,51 (1 ),30-7

Zhang, J X; Cushion, M T; Giuntoli, D; Hong, S T; Meade, J C; Smulian, A G; Stringer, S L; Stringer, J J Analysis of the Pneumocystis carinii genome. The Journal of protozoology, ,38 (6 ),69S

Cushion, M T; Linke, M J Factors influencing Pneumocystis infection in the immunocompromised rat. The Journal of protozoology, ,38 (6 ),133S-135S

Kaneshiro, E S; Wu, Y P; Cushion, M T Assays for testing Pneumocystis carinii viability. The Journal of protozoology, ,38 (6 ),85S-87S

Stringer, J R; Stringer, S L; Zhang, J; Baughman, R; Smulian, A G; Cushion, M T Molecular genetic distinction of Pneumocystis carinii from rats and humans. The Journal of eukaryotic microbiology, ,40 (6 ),733-41

Cushion, M T; Walzer, P D Closing comments: a retrospective perspective 4th International Workshop on Pneumocystis. The Journal of eukaryotic microbiology, ,43 (5 ),60S-61S

Keely, S P; Cushion, M T; Stringer, J R Stability of four genetic loci in Pneumocystis carinii sp. f. carinii. The Journal of eukaryotic microbiology, ,43 (5 ),49S

Cushion, M T; Orr, S Kinetics of 2 genetically distinct Pneumocystis carinii populations in rat colonies. The Journal of eukaryotic microbiology, ,43 (5 ),46S

Langreth, S G; Kim, G M; Cushion, M T Evaluation of procedures used to isolate Pneumocystis carinii from infected rat lungs. The Journal of eukaryotic microbiology, ,41 (5 ),95S-96S

Chen, F; Cushion, M T Use of fluorescent probes to investigate the metabolic state of Pneumocystis carinii mitochondria. The Journal of eukaryotic microbiology, ,41 (5 ),79S

Andrews, R P; Theus, S A; Cushion, M T; Walzer, P D Comparison of the antigenic recognition of the major surface glycoprotein of Pneumocystis carinii isolated from different rat strains. The Journal of eukaryotic microbiology, ,41 (5 ),72S

Weinberg, G A; O'Gara, M J; Cushion, M T Coinfection of rats with genetically diverse forms of Pneumocystis carinii demonstrated by P. carinii inosine monophosphate dehydrogenase gene polymorphism. The Journal of eukaryotic microbiology, ,41 (5 ),119S

Weinberg, G A; Dykstra, C C; Durant, P J; Cushion, M T Chromosomal localization of 20 genes to five distinct pulsed field gel karyotypic forms of rat Pneumocystis carinii. The Journal of eukaryotic microbiology, ,41 (5 ),117S

Vasquez, J; Smulian, A G; Linke, M J; Cushion, M T Antigenic differences among genetically distinct types of rat-derived Pneumocystis carinii. The Journal of eukaryotic microbiology, ,41 (5 ),114S

Settnes, O P; Nielsen, P B; Bucala, R; Linke, M J; Cushion, M T A survey of birds in Denmark for the presence of Pneumocystis carinii. Avian diseases, ,38 (1 ),1-10

Cushion, M T; Orr, S; Arnold, J Interactions between 2 Pneumocystis populations within the same host. The Journal of eukaryotic microbiology, ,44 (6 ),9S

Arnold, J; Cushion, M T Constructing a physical map of the Pneumocystis genome. The Journal of eukaryotic microbiology, ,44 (6 ),8S

Cushion, M T; Arnold, J Proposal for a Pneumocystis genome project. The Journal of eukaryotic microbiology, ,44 (6 ),7S

Stringer, J R; Wakefield, A E; Cushion, M T; Dei-Cas, E Pneumocystis taxonomy and nomenclature: an update. The Journal of eukaryotic microbiology, ,44 (6 ),5S-6S

Cushion, Melanie T; Keely, Scott P; Stringer, James R Molecular and phenotypic description of Pneumocystis wakefieldiae sp. nov., a new species in rats. Mycologia, ,96 (3 ),429-38

Sinai, Anthony P; Kaneshiro, Edna S; Ward, Honorine; Weiss, Louis M; Cushion, Melanie T (2012. )The state of research for AIDS-associated opportunistic infections and the importance of sustaining smaller research communities.Eukaryotic cell, ,11 (2 ),90-7

Maciejewska, Dorota; Zabinski, Jerzy; Ka?mierczak, Pawel; Rezler, Mateusz; Krassowska-?wiebocka, Barbara; Collins, Margaret S; Cushion, Melanie T (2012. )Analogs of pentamidine as potential anti-Pneumocystis chemotherapeutics.European journal of medicinal chemistry, ,48 ,164-73

Cushion, Melanie T; Collins, Margaret S (2011. )Susceptibility of Pneumocystis to echinocandins in suspension and biofilm cultures.Antimicrobial agents and chemotherapy, ,55 (10 ),4513-8

Lobo, Maria Luísa; Esteves, Francisco; de Sousa, Bruno; Cardoso, Fernando; Cushion, Melanie T; Antunes, Francisco; Matos, Olga (2013. )Therapeutic potential of caspofungin combined with trimethoprim-sulfamethoxazole for pneumocystis pneumonia: a pilot study in mice.PloS one, ,8 (8 ),e70619

Weiss, Louis M; Cushion, Melanie T; Didier, Elizabeth; Xiao, Lihua; Marciano-Cabral, Francine; Sinai, Anthony P; Matos, Olga; Calderon, Enrique J; Kaneshiro, Edna S The 12th International Workshops on Opportunistic Protists (IWOP-12).The Journal of eukaryotic microbiology, ,60 (3 ),298-308

Cushion, Melanie T; Keely, Scott P (2013. )Assembly and annotation of Pneumocystis jirovecii from the human lung microbiome.mBio, ,4 (2 ),e00224

Linke, Michael J; Ashbaugh, Alan; Collins, Margaret S; Lynch, Keeley; Cushion, Melanie T (2013. )Characterization of a distinct host response profile to Pneumocystis murina asci during clearance of pneumocystis pneumonia.Infection and immunity, ,81 (3 ),984-95

Porollo, Aleksey; Meller, Jaroslaw; Joshi, Yogesh; Jaiswal, Vikash; Smulian, A George; Cushion, Melanie T (2012. )Analysis of current antifungal agents and their targets within the Pneumocystis carinii genome. Current drug targets, ,13 (12 ),1575-85

Tachado, Souvenir D; Zhang, Jianmin; Zhu, Jinping; Patel, Naimish; Cushion, Melanie; Koziel, Henry (2007. )Pneumocystis-mediated IL-8 release by macrophages requires coexpression of mannose receptors and TLR2.Journal of leukocyte biology, ,81 (1 ),205-11

Dei-Cas, Eduardo; Chabé, Magali; Moukhlis, Raya; Durand-Joly, Isabelle; Aliouat, El Moukhtar; Stringer, James R; Cushion, Melanie; Noël, Christophe; de Hoog, G Sybren; Guillot, Jacques; Viscogliosi, Eric (2006. )Pneumocystis oryctolagi sp. nov., an uncultured fungus causing pneumonia in rabbits at weaning: review of current knowledge, and description of a new taxon on genotypic, phylogenetic and phenotypic bases.FEMS microbiology reviews, ,30 (6 ),853-71

Zhang, Jianmin; Tachado, Souvenir D; Patel, Naimish; Zhu, Jinping; Imrich, Amy; Manfruelli, Pascal; Cushion, Melanie; Kinane, T Bernard; Koziel, Henry (2005. )Negative regulatory role of mannose receptors on human alveolar macrophage proinflammatory cytokine release in vitro.Journal of leukocyte biology, ,78 (3 ),665-74

Zhang, Jianmin; Zhu, Jinping; Bu, Xia; Cushion, Melanie; Kinane, T Bernard; Avraham, Hava; Koziel, Henry (2005. )Cdc42 and RhoB activation are required for mannose receptor-mediated phagocytosis by human alveolar macrophages.Molecular biology of the cell, ,16 (2 ),824-34

Zhang, Jianmin; Zhu, Jinping; Imrich, Amy; Cushion, Melanie; Kinane, T Bernard; Koziel, Henry (2004. )Pneumocystis activates human alveolar macrophage NF-kappaB signaling through mannose receptors.Infection and immunity, ,72 (6 ),3147-60

De Lucca, A J; Bland, J M; Vigo, C B; Cushion, M; Selitrennikoff, C P; Peter, J; Walsh, T J (2002. )CAY-I, a fungicidal saponin from Capsicum sp. fruit. Medical mycology, ,40 (2 ),131-7

Smulian, A G; Ryan, M; Staben, C; Cushion, M (1996. )Signal transduction in Pneumocystis carinii: characterization of the genes (pcg1) encoding the alpha subunit of the G protein (PCG1) of Pneumocystis carinii carinii and Pneumocystis carinii ratti. Infection and immunity, ,64 (3 ),691-701

Sloand, E; Laughon, B; Armstrong, M; Bartlett, M S; Blumenfeld, W; Cushion, M; Kalica, A; Kovacs, J A; Martin, W; Pitt, E The challenge of Pneumocystis carinii culture. The Journal of eukaryotic microbiology, ,40 (2 ),188-95

Cushion, Melanie T; Collins, Margaret S; Sesterhenn, Thomas; Porollo, Aleksey; Vadukoot, Anish Kizhakkekkara; Merino, Edward J (2016. )Functional Characterization of Pneumocystis carinii Inositol Transporter 1.mBio, ,7 (6 ),

Baker, Frazier N; Cushion, Melanie T; Porollo, Aleksey (2016. )A Quantitative Model to Estimate Drug Resistance in Pathogens.Journal of fungi (Basel, Switzerland), ,2 (4 ),

Koselny, Kristy; Green, Julianne; DiDone, Louis; Halterman, Justin P; Fothergill, Annette W; Wiederhold, Nathan P; Patterson, Thomas F; Cushion, Melanie T; Rappelye, Chad; Wellington, Melanie; Krysan, Damian J (2016. )The Celecoxib Derivative AR-12 Has Broad-Spectrum Antifungal Activity In Vitro and Improves the Activity of Fluconazole in a Murine Model of Cryptococcosis.Antimicrobial agents and chemotherapy, ,60 (12 ),7115-7127

Calderon, Enrique J; Cushion, Melanie T; Xiao, Lihua; Lorenzo-Morales, Jacob; Matos, Olga; Kaneshiro, Edna S; Weiss, Louis M The 13th International Workshops on Opportunistic Protists (IWOP13).The Journal of eukaryotic microbiology, ,62 (5 ),701-9

Mor, Visesato; Rella, Antonella; Farnoud, Amir M; Singh, Ashutosh; Munshi, Mansa; Bryan, Arielle; Naseem, Shamoon; Konopka, James B; Ojima, Iwao; Bullesbach, Erika; Ashbaugh, Alan; Linke, Michael J; Cushion, Melanie; Collins, Margaret; Ananthula, Hari Krishna; Sallans, Larry; Desai, Pankaj B; Wiederhold, Nathan P; Fothergill, Annette W; Kirkpatrick, William R; Patterson, Thomas; Wong, Lai Hong; Sinha, Sunita; Giaever, Guri; Nislow, Corey; Flaherty, Patrick; Pan, Xuewen; Cesar, Gabriele Vargas; de Melo Tavares, Patricia; Frases, Susana; Miranda, Kildare; Rodrigues, Marcio L; Luberto, Chiara; Nimrichter, Leonardo; Del Poeta, Maurizio (2015. )Identification of a New Class of Antifungals Targeting the Synthesis of Fungal Sphingolipids.mBio, ,6 (3 ),e00647

Porollo, Aleksey; Sesterhenn, Thomas M; Collins, Margaret S; Welge, Jeffrey A; Cushion, Melanie T (2014. )Comparative genomics of pneumocystis species suggests the absence of genes for myo-inositol synthesis and reliance on inositol transport and metabolism.mBio, ,5 (6 ),e01834

Hauser, Philippe M; Cushion, Melanie T (2018. )Is sex necessary for the proliferation and transmission of Pneumocystis?.PLoS pathogens, ,14 (12 ),e1007409

Cushion, Melanie T; Limper, Andrew H; Porollo, Aleksey; Saper, Vivian E; Sinai, Anthony P; Weiss, Louis M (2018. )The 14th International Workshops on Opportunistic Protists (IWOP 14).The Journal of eukaryotic microbiology, ,65 (6 ),934-939

Cushion, Melanie T (2018. )Reply to Nevez et al., "The Fascinating Echinocandin-Treated Mouse Model of Pneumocystis murina To Understand Pneumocystis jirovecii".Antimicrobial agents and chemotherapy, ,62 (8 ),

Liu, Guan-Sheng; Ballweg, Richard; Ashbaugh, Alan; Zhang, Yin; Facciolo, Joseph; Cushion, Melanie T; Zhang, Tongli (2018. )A quantitative systems pharmacology (QSP) model for Pneumocystis treatment in mice.BMC systems biology, ,12 (1 ),77

Lazzarini, Cristina; Haranahalli, Krupanandan; Rieger, Robert; Ananthula, Hari Krishna; Desai, Pankaj B; Ashbaugh, Alan; Linke, Michael J; Cushion, Melanie T; Ruzsicska, Bela; Haley, John; Ojima, Iwao; Del Poeta, Maurizio (2018. )Acylhydrazones as Antifungal Agents Targeting the Synthesis of Fungal Sphingolipids.Antimicrobial agents and chemotherapy, ,62 (5 ),

Cushion, Melanie T; Ashbaugh, Alan; Hendrix, Keeley; Linke, Michael J; Tisdale, Nikeya; Sayson, Steven G; Porollo, Aleksey (2018. )Gene Expression of Pneumocystis murina after Treatment with Anidulafungin Results in Strong Signals for Sexual Reproduction, Cell Wall Integrity, and Cell Cycle Arrest, Indicating a Requirement for Ascus Formation for Proliferation.Antimicrobial agents and chemotherapy, ,62 (5 ),

Mor, Visesato; Rella, Antonella; Farnoud, Amir M; Singh, Ashutosh; Munshi, Mansa; Bryan, Arielle; Naseem, Shamoon; Konopka, James B; Ojima, Iwao; Bullesbach, Erika; Ashbaugh, Alan; Linke, Michael J; Cushion, Melanie; Collins, Margaret; Ananthula, Hari Krishna; Sallans, Larry; Desai, Pankaj B; Wiederhold, Nathan P; Fothergill, Annette W; Kirkpatrick, William R; Patterson, Thomas; Wong, Lai Hong; Sinha, Sunita; Giaever, Guri; Nislow, Corey; Flaherty, Patrick; Pan, Xuewen; Cesar, Gabriele Vargas; de Melo Tavares, Patricia; Frases, Susana; Miranda, Kildare; Rodrigues, Marcio L; Luberto, Chiara; Nimrichter, Leonardo; Del Poeta, Maurizio (2018. )Erratum for Mor et al., "Identification of a New Class of Antifungals Targeting the Synthesis of Fungal Sphingolipids".mBio, ,9 (2 ),

Honors and Awards

1973 -1977 Academic Trustee Scholarship, St. Joseph's College

1977 -1981 Academic and Tuition Scholarship, St. Thomas Institute

1996 Seymour H. Hutner Young Investigator Awardee; Society of Protozoologists

2000 ASM-UNESCO Visiting Scholar Program; University of Chile, Santiago; Dr. Sergio sponsor

2005 -2010 Research Career Scientist Award; Veterans Affairs Administration

2007 Elected Fellow of the Graduate School, University of Cincinnati

2009 Elected to Fellowship in the American Academy of Microbiology

2010 -2015 Research Career Scientist Award; Veterans Affairs Administration

2010 Executive Leadership in Academic Medicine; selected as a member of the Class of 2010

Positions and Work Experience

1981 -1985 Postdoctoral fellow, laboratory of Dr. Peter Walzer, University of Cincinnati College of Medicine and VAMC, Cincinnati, OH

1985 -1987 Instructor, Department of Pathology, University of Cincinnati College of Medicine, Cincinnati, OH

1985 -1987 Research Instructor of Medicine, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH

1986 -To Present Research Microbiologist, VA Medical Center, Cincinnati, OH

1987 -1992 Research Assistant Professor of Medicine, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH

1992 -1994 Assistant Professor of Medicine, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH

1994 -2001 Associate Professor of Medicine, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH

1998 - Associate Professor, Granted Tenure,

2001 -To Present Professor, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH

2006 -To Present Associate Chair for Research, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, OH

Keywords

Organisms(MeSH B),Diseases(MeSH C),Chemicals and Drugs(MeSH D),Biological Sciences(MeSH G),Algae and Fungi(MeSH B05),Bacterial Infections and Mycoses(MeSH C01),Parasitic Diseases(MeSH C03),Respiratory Tract Diseases(MeSH C08),Animal Diseases(MeSH C22),Anti-Infective Agents(MeSH D20),Biochemical Phenomena, Metabolism, and Nutrition(MeSH G06)

Research and Practice Interests

Fungi in the genus Pneumocystis cause an oftentimes lethal pneumonia (PCP)  in humans and other mammals with compromised immune status. The niche of these fungi include patients with underlying chronic diseases such as COPD or HIV and those receiving anti-inflammatory or immunosuppressive agents. PCP is not responsive to standard antifungal therapy with few treatment alternatives besides tri­methoprim-sulfamethoxazole. My laboratory focuses on  pre-clinical drug development that includes discovery of potential new targets by understanding the metabolism of these obli­gate fungi;  in silico or in vitro screening of inhibitors to identify potential new drugs; evaluation of toxicity in vitro, and eventually evaluation in rodent animal models of this fungal pneumonia. Approaches used for discovery include RNA_seq, comparative genomics, and validation by qRT-PCR; high-throughput screening using yeast expression as well as new approaches such as alveolar organoids to grow these unculturable fungi outside the lung.   

Contact Information

Academic - CARE / Crawley Building
Room E870
Cincinnati  Ohio, 45267
Phone: 513-558-7540
Fax: 513-558-4408
melanie.cushion@uc.edu