Gary E Dean, Ph.D.

Bachelor's Degree: U. California @ Santa Cruz Santa Cruz, CA, 1975 (Biology & Chemistry)

Doctoral Degree: Yale University New Haven, CT, 1983 (Molecular Biophysics & Biochemistry)

Postdoctoral Fellow: Yale University New Haven, Ct , 1985

My primary objectives are to understand: the biogenesis, structure, and regulation of expression of chromaffin granule membrane proteins, including synaptotagmin; the biogenic amine transporter; the vacuolar ATPase; the physiology and molecular genetics of histoplasma capsulatum. In addition, I am involved in the development of science curricula in the Cincinnati public schools, currently using the vacuolar ATPase of Saccharomyces cerevisiae as a model system for teaching modern molecular genetic concepts.

1985 - Assistant Professor of Microbiology and Molecular Genetics, University of Cincinnati, MSB

Investigators:Dean, Gary 09-01-2005 -08-31-2006 Meridian Bioscience Inc. Histoplasma Growth Role:PI $22,340.00 Closed Level:Industry

Grant: #2003-UC-14 BRTT-01-A0-S0-E0 Investigators:Dean, Gary 07-01-2002 -06-30-2005 Ohio Board of Regents Bridging Teaching and Research: Graduate Students in the Schools Role:PI $127,500.00 Closed Level:State of Ohio

Grant: #4-R25-RR-12357-03-A0-S0-E1 Investigators:Dean, Gary 09-01-1998 -08-31-2002 National Center for Research Resources Yeast Mutants as an Educational Tool, Phase I Role:PI $802,991.00 Closed Level:Federal

Grant: #S-845-010-002 / FA8650-08-C-6832 Investigators:Dean, Gary; Hassett, Daniel; Kotb, Malak; Lamkin, Thomas; Nelson, Sandra; Papoian, Ruben; Seibel, William; Strasser, Jane; Wortman, Matthew 08-26-2009 -02-11-2011 Defense Threat Reduction Agency INSIGHTS INteractome Science in Genomic High Throughput Studies Role:Collaborator $3,840,985.00 Active Level:Federal

Grant: #ADIOS S-845-001-005 / FA8650-08-C-6832 Investigators:Dean, Gary 04-01-2011 -11-30-2012 Air Force Research Laboratory ADIOS Pilot Study Role:PI $192,921.00 Active Level:Federal

Grant: #923-100-003 Investigators:Dean, Gary 01-01-2011 -07-31-2012 Defense Threat Reduction Agency INSIGHTS S-923-100-003 Role:PI $1,680,000.00 Active Level:Federal

Grant: #S 923-200-003 Investigators:Bangar, Hansraj; Dean, Gary 07-01-2012 -11-30-2012 Department of the Air Force BSL3 Expertise, Support and Screening Activities Role:PI $35,535.00 Active Level:Federal

Grant: #226-6273-UC1 / FA8650-12-C-6273 Investigators:Dean, Gary 09-03-2012 -08-30-2013 Department of the Air Force Advances in Bioscience for Airmen Performance, Bio-Surveillance Technologies Role:PI $169,568.31 Active Level:Federal

Grant: #OSU / ODH Investigators:Dean, Gary; Wendell, David 12-01-2020 -06-30-2021 Ohio Department of Health Dormitory wastewater monitoring of SARS-CoV-2 at Universities and Colleges in the State of Ohio - subaward Role:Collaborator 98934.00 Active Level:Ohio, State of

Peer Reviewed Publications

Bowling, Bethany Vice; Acra, Erin E; Wang, Lihshing; Myers, Melanie F; Dean, Gary E; Markle, Glenn C; Moskalik, Christine L; Huether, Carl A  (2008). Development and evaluation of a genetics literacy assessment instrument for undergraduates. Genetics, 178 (1) , 15-22More Information

Sartor, Maureen A; Zorn, Aaron M; Schwanekamp, Jennifer A; Halbleib, Danielle; Karyala, Saikumar; Howell, Michael L; Dean, Gary E; Medvedovic, Mario; Tomlinson, Craig R  (2006). A new method to remove hybridization bias for interspecies comparison of global gene expression profiles uncovers an association between mRNA sequence divergence and differential gene expression in Xenopus. Nucleic acids research, 34 (1) , 185-200More Information

Hewitt, Eric W; Tao, Jing-Xian; Strasser, Jane E; Cutler, Daniel F; Dean, Gary E  (2002). Synaptotagmin I-DeltaC2B. A novel synaptotagmin isoform with a single C2 domain in the bovine adrenal medulla. Biochimica et biophysica acta, 1561 (1) , 76-90

Howell, M L; Alsabbagh, E; Ma, J F; Ochsner, U A; Klotz, M G; Beveridge, T J; Blumenthal, K M; Niederhoffer, E C; Morris, R E; Needham, D; Dean, G E; Wani, M A; Hassett, D J  (2000). AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa, is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide. Journal of bacteriology, 182 (16) , 4545-56

Pance, A; Morgan, K; Guest, P C; Bowers, K; Dean, G E; Cutler, D F; Jackson, A P  (1999). A PC12 variant lacking regulated secretory organelles: aberrant protein targeting and evidence for a factor inhibiting neuroendocrine gene expression. Journal of neurochemistry, 73 (1) , 21-30

Strasser, J E; Newman, S L; Ciraolo, G M; Morris, R E; Howell, M L; Dean, G E  (1999). Regulation of the macrophage vacuolar ATPase and phagosome-lysosome fusion by Histoplasma capsulatum. Journal of immunology (Baltimore, Md. : 1950), 162 (10) , 6148-54

Zemlan, F P; Rosenberg, W S; Luebbe, P A; Campbell, T A; Dean, G E; Weiner, N E; Cohen, J A; Rudick, R A; Woo, D  (1999). Quantification of axonal damage in traumatic brain injury: affinity purification and characterization of cerebrospinal fluid tau proteins. Journal of neurochemistry, 72 (2) , 741-50

Hassett, D J; Howell, M L; Ochsner, U A; Vasil, M L; Johnson, Z; Dean, G E  (1997). An operon containing fumC and sodA encoding fumarase C and manganese superoxide dismutase is controlled by the ferric uptake regulator in Pseudomonas aeruginosa: fur mutants produce elevated alginate levels. Journal of bacteriology, 179 (5) , 1452-9

Hassett, D J; Howell, M L; Sokol, P A; Vasil, M L; Dean, G E  (1997). Fumarase C activity is elevated in response to iron deprivation and in mucoid, alginate-producing Pseudomonas aeruginosa: cloning and characterization of fumC and purification of native fumC. Journal of bacteriology, 179 (5) , 1442-51

McLaughlin, L; Zemlan, F P; Dean, G E  (1997). Identification of microtubule-associated protein tau isoforms in Alzheimer's paired helical filaments. Brain research bulletin, 43 (5) , 501-8

Zemlan, F P; Dean, G E  (1996). Monoclonal antibody PHF-9 recognizes phosphorylated serine 404 of tau protein and labels paired helical filaments. Journal of neuroscience research, 46 (1) , 90-7More Information

Laitala-Leinonen, T; Howell, M L; Dean, G E; Väänänen, H K  (1996). Resorption-cycle-dependent polarization of mRNAs for different subunits of V-ATPase in bone-resorbing osteoclasts. Molecular biology of the cell, 7 (1) , 129-42

Pan, Y X; Cheng, J; Xu, J; Rossi, G; Jacobson, E; Ryan-Moro, J; Brooks, A I; Dean, G E; Standifer, K M; Pasternak, G W  (1995). Cloning and functional characterization through antisense mapping of a kappa 3-related opioid receptor. Molecular pharmacology, 47 (6) , 1180-8

Perler, F B; Davis, E O; Dean, G E; Gimble, F S; Jack, W E; Neff, N; Noren, C J; Thorner, J; Belfort, M  (1994). Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature. Nucleic acids research, 22 (7) , 1125-7

Howell, M; Shirvan, A; Stern-Bach, Y; Steiner-Mordoch, S; Strasser, J E; Dean, G E; Schuldiner, S  (1994). Cloning and functional expression of a tetrabenazine sensitive vesicular monoamine transporter from bovine chromaffin granules. FEBS letters, 338 (1) , 16-22

Zemlan, F P; Vogelsang, G D; McLaughlin, L; Dean, G E  (1994). Alzheimer's paired helical filaments: amyloid precursor protein epitope mapping. Brain research bulletin, 33 (4) , 387-92

Schafer, M P; Dean, G E  (1993). Cloning and sequence analysis of an H(+)-ATPase-encoding gene from the human dimorphic pathogen Histoplasma capsulatum. Gene, 136 (1-2) , 295-300

Pan, Y X; Gu, H H; Xu, J; Dean, G E  (1993). Saccharomyces cerevisiae expression of exogenous vacuolar ATPase subunits B. Biochimica et biophysica acta, 1151 (2) , 175-85

Gu, H H; Xu, J; Gallagher, M; Dean, G E  (1993). Peptide splicing in the vacuolar ATPase subunit A from Candida tropicalis. The Journal of biological chemistry, 268 (10) , 7372-81

Pan, Y X; Xu, J; Strasser, J E; Howell, M; Dean, G E  (1991). Structure and expression of subunit A from the bovine chromaffin cell vacuolar ATPase. FEBS letters, 293 (1-2) , 89-92

Gu, H H; Gallagher, M J; Rupkey, S; Dean, G E  (1990). Gene and derived peptide sequences for C. tropicalis vacuolar ATPase subunit b. Nucleic acids research, 18 (24) , 7446

Vogelsang, G D; Zemlan, F P; Dean, G E  (1990). Purification and solubilization of paired helical filaments from Alzheimer brains. Journal of neurochemistry, 54 (1) , 148-55

Vogelsang, G D; Zemlan, F P; Dean, G E  (1989). Hyperpurification of paired helical filaments reveals elevations in hydroxyproline content and a core structure related peptide fragment. Progress in clinical and biological research, 317, 791-800

Cuppoletti, J; Strasser, J E; Dean, G E  (1988). An immunoreactive 8-azido ATP-labeled protein common to the lysosomal and chromaffin granule membrane. Biochimica et biophysica acta, 946 (1) , 33-9

Galloway, C J; Dean, G E; Fuchs, R; Mellman, I  (1988). Analysis of endosome and lysosome acidification in vitro. Methods in enzymology, 157, 601-11

Homma, M; Aizawa, S; Dean, G E; Macnab, R M  (1987). Identification of the M-ring protein of the flagellar motor of Salmonella typhimurium. Proceedings of the National Academy of Sciences of the United States of America, 84 (21) , 7483-7

Dean, G E; Nelson, P J; Agnew, W S; Rudnick, G  (1987). Hydrodynamic properties of the chromaffin granule hydrogen ion pumping adenosinetriphosphatase. Biochemistry, 26 (8) , 2301-5

Dean, G E; Nelson, P J; Rudnick, G  (1986). Characterization of native and reconstituted hydrogen ion pumping adenosinetriphosphatase of chromaffin granules. Biochemistry, 25 (17) , 4918-25

Marsh, M; Griffiths, G; Dean, G E; Mellman, I; Helenius, A  (1986). Three-dimensional structure of endosomes in BHK-21 cells. Proceedings of the National Academy of Sciences of the United States of America, 83 (9) , 2899-903

Stader, J; Matsumura, P; Vacante, D; Dean, G E; Macnab, R M  (1986). Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. Journal of bacteriology, 166 (1) , 244-52

Aizawa, S I; Dean, G E; Jones, C J; Macnab, R M; Yamaguchi, S  (1985). Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium. Journal of bacteriology, 161 (3) , 836-49

Rudnick, G; Humphreys, C J; Dean, G E  (1985). Serotonin transport by platelet plasma and granule membranes. Annals of the New York Academy of Sciences, 456, 277-8

Dean, G E; Macnab, R M; Stader, J; Matsumura, P; Burks, C  (1984). Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli. Journal of bacteriology, 159 (3) , 991-9

Dean, G E; Fishkes, H; Nelson, P J; Rudnick, G  (1984). The hydrogen ion-pumping adenosine triphosphatase of platelet dense granule membrane. Differences from F1F0- and phosphoenzyme-type ATPases. The Journal of biological chemistry, 259 (15) , 9569-74

Nelson, P J; Dean, G E; Aronson, P S; Rudnick, G  (1983). Hydrogen ion cotransport by the renal brush border glutamate transporter. Biochemistry, 22 (23) , 5459-63

Galloway, C J; Dean, G E; Marsh, M; Rudnick, G; Mellman, I  (1983). Acidification of macrophage and fibroblast endocytic vesicles in vitro. Proceedings of the National Academy of Sciences of the United States of America, 80 (11) , 3334-8

Dean, G E; Aizawa, S I; Macnab, R M  (1983). flaAII (motC, cheV) of Salmonella typhimurium is a structural gene involved in energization and switching of the flagellar motor. Journal of bacteriology, 154 (1) , 84-91

Bowling, B.V., C.A. Huether, L. Wang, M.F. Myers, G.C. Markle, G.E. Dean, E.E. Acra, F.P. Wray, G.A. Jacob  (2008). Genetic Literacy of Undergraduate Non-Science Majors and the Impact of Introductory Biology and Genetics Courses. BioScience, 58 (7) , 654

Weingart, C., Broitman-Maduro, G., Dean, G., Newman, S., Peppler, M., and Weiss, A.  (1999). Effect of labeling treatments on phagocytosis of Bordatella pertussis by human neutrophils. Infection & Immunity, 67 (8) , 4264

Su, Shengchang; Bangar, Hansraj; Saldanha, Roland; Pemberton, Adin; Aronow, Bruce; Dean, Gary E; Lamkin, Thomas J; Hassett, Daniel J  (2014). Construction and characterization of stable, constitutively expressed, chromosomal green and red fluorescent transcriptional fusions in the select agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei. MicrobiologyOpen, 3 (5) , 610-29More Information

Molecular Biologist,Biochemist,Molecular Geneticist,Cell Biologist,Energetics,Molecular Interaction,Protein Purification,Protein Synthesis,Nucleic Acid Synthesis,Cell Organelles,Cell Substructures,Lysosomes,Membranes,Membrane Transport,Phagocytosis,Endocrine System,Nervous System,Fungal Physiology,Vertebrate Endocrine System,Adrenal Gland,Science Education

Academic - Dept. Molecular Genetics, Biochemistry & Microbiology
231 Albert Sabin Way
Cincinnati  Ohio, 45267-0524
Phone: 513-558-0065
Fax: 513-558-0014
gary.dean@uc.edu