Gary Dean

Gary E Dean

Professor-Emeritus

Medical Sciences Building

2258

COM Molecular Genetics - 0524

Dept. Molecular Genetics, Biochemistry & Microbiology
231 Albert Sabin Way
Cincinnati, Ohio 45267-0524

Education

B.A., U. California @ Santa Cruz Santa Cruz, CA, 1975 (Biology & Chemistry)

PhD, Yale University New Haven, CT, 1983 (Molecular Biophysics & Biochemistry)

Positions and Work Experience

1985 - Assistant Professor of Microbiology and Molecular Genetics, University of Cincinnati, MSB

Research and Practice Interests

My primary objectives are to understand: the biogenesis, structure, and regulation of expression of chromaffin granule membrane proteins, including synaptotagmin; the biogenic amine transporter; the vacuolar ATPase; the physiology and molecular genetics of histoplasma capsulatum. In addition, I am involved in the development of science curricula in the Cincinnati public schools, currently using the vacuolar ATPase of Saccharomyces cerevisiae as a model system for teaching modern molecular genetic concepts.

Research Support

Investigators:Dean, Gary 09-01-2005 -08-31-2006 Meridian Bioscience Inc. Histoplasma Growth Role:PI $22,340.00 Closed Level:Industry

Grant: #2003-UC-14 BRTT-01-A0-S0-E0 Investigators:Dean, Gary 07-01-2002 -06-30-2005 Ohio Board of Regents Bridging Teaching and Research: Graduate Students in the Schools Role:PI $127,500.00 Closed Level:State of Ohio

Grant: #4-R25-RR-12357-03-A0-S0-E1 Investigators:Dean, Gary 09-01-1998 -08-31-2002 National Center for Research Resources Yeast Mutants as an Educational Tool, Phase I Role:PI $802,991.00 Closed Level:Federal

Grant: #S-845-010-002 / FA8650-08-C-6832 Investigators:Dean, Gary; Hassett, Daniel; Kotb, Malak; Lamkin, Thomas; Nelson, Sandra; Papoian, Ruben; Seibel, William; Strasser, Jane; Wortman, Matthew 08-26-2009 -02-11-2011 Defense Threat Reduction Agency INSIGHTS INteractome Science in Genomic High Throughput Studies Role:Collaborator $3,840,985.00 Active Level:Federal

Grant: #ADIOS S-845-001-005 / FA8650-08-C-6832 Investigators:Dean, Gary 04-01-2011 -11-30-2012 Air Force Research Laboratory ADIOS Pilot Study Role:PI $192,921.00 Active Level:Federal

Grant: #923-100-003 Investigators:Dean, Gary 01-01-2011 -07-31-2012 Defense Threat Reduction Agency INSIGHTS S-923-100-003 Role:PI $1,680,000.00 Active Level:Federal

Grant: #S 923-200-003 Investigators:Bangar, Hansraj; Dean, Gary 07-01-2012 -11-30-2012 Department of the Air Force BSL3 Expertise, Support and Screening Activities Role:PI $35,535.00 Active Level:Federal

Grant: #226-6273-UC1 / FA8650-12-C-6273 Investigators:Dean, Gary 09-03-2012 -08-30-2013 Department of the Air Force Advances in Bioscience for Airmen Performance, Bio-Surveillance Technologies Role:PI $169,568.31 Active Level:Federal

Publications

Peer Reviewed Publications

Bowling, Bethany Vice; Acra, Erin E; Wang, Lihshing; Myers, Melanie F; Dean, Gary E; Markle, Glenn C; Moskalik, Christine L; Huether, Carl A (2008. ) Development and evaluation of a genetics literacy assessment instrument for undergraduates.Genetics, , 178 (1 ) ,15-22

Sartor, Maureen A; Zorn, Aaron M; Schwanekamp, Jennifer A; Halbleib, Danielle; Karyala, Saikumar; Howell, Michael L; Dean, Gary E; Medvedovic, Mario; Tomlinson, Craig R (2006. ) A new method to remove hybridization bias for interspecies comparison of global gene expression profiles uncovers an association between mRNA sequence divergence and differential gene expression in Xenopus.Nucleic acids research, , 34 (1 ) ,185-200

Hewitt, Eric W; Tao, Jing-Xian; Strasser, Jane E; Cutler, Daniel F; Dean, Gary E (2002. ) Synaptotagmin I-DeltaC2B. A novel synaptotagmin isoform with a single C2 domain in the bovine adrenal medulla. Biochimica et biophysica acta, , 1561 (1 ) ,76-90

Howell, M L; Alsabbagh, E; Ma, J F; Ochsner, U A; Klotz, M G; Beveridge, T J; Blumenthal, K M; Niederhoffer, E C; Morris, R E; Needham, D; Dean, G E; Wani, M A; Hassett, D J (2000. ) AnkB, a periplasmic ankyrin-like protein in Pseudomonas aeruginosa, is required for optimal catalase B (KatB) activity and resistance to hydrogen peroxide. Journal of bacteriology, , 182 (16 ) ,4545-56

Pance, A; Morgan, K; Guest, P C; Bowers, K; Dean, G E; Cutler, D F; Jackson, A P (1999. ) A PC12 variant lacking regulated secretory organelles: aberrant protein targeting and evidence for a factor inhibiting neuroendocrine gene expression. Journal of neurochemistry, , 73 (1 ) ,21-30

Strasser, J E; Newman, S L; Ciraolo, G M; Morris, R E; Howell, M L; Dean, G E (1999. ) Regulation of the macrophage vacuolar ATPase and phagosome-lysosome fusion by Histoplasma capsulatum. Journal of immunology (Baltimore, Md. : 1950), , 162 (10 ) ,6148-54

Zemlan, F P; Rosenberg, W S; Luebbe, P A; Campbell, T A; Dean, G E; Weiner, N E; Cohen, J A; Rudick, R A; Woo, D (1999. ) Quantification of axonal damage in traumatic brain injury: affinity purification and characterization of cerebrospinal fluid tau proteins. Journal of neurochemistry, , 72 (2 ) ,741-50

Hassett, D J; Howell, M L; Ochsner, U A; Vasil, M L; Johnson, Z; Dean, G E (1997. ) An operon containing fumC and sodA encoding fumarase C and manganese superoxide dismutase is controlled by the ferric uptake regulator in Pseudomonas aeruginosa: fur mutants produce elevated alginate levels. Journal of bacteriology, , 179 (5 ) ,1452-9

Hassett, D J; Howell, M L; Sokol, P A; Vasil, M L; Dean, G E (1997. ) Fumarase C activity is elevated in response to iron deprivation and in mucoid, alginate-producing Pseudomonas aeruginosa: cloning and characterization of fumC and purification of native fumC. Journal of bacteriology, , 179 (5 ) ,1442-51

McLaughlin, L; Zemlan, F P; Dean, G E (1997. ) Identification of microtubule-associated protein tau isoforms in Alzheimer's paired helical filaments. Brain research bulletin, , 43 (5 ) ,501-8

Zemlan, F P; Dean, G E (1996. ) Monoclonal antibody PHF-9 recognizes phosphorylated serine 404 of tau protein and labels paired helical filaments.Journal of neuroscience research, , 46 (1 ) ,90-7

Laitala-Leinonen, T; Howell, M L; Dean, G E; Väänänen, H K (1996. ) Resorption-cycle-dependent polarization of mRNAs for different subunits of V-ATPase in bone-resorbing osteoclasts. Molecular biology of the cell, , 7 (1 ) ,129-42

Pan, Y X; Cheng, J; Xu, J; Rossi, G; Jacobson, E; Ryan-Moro, J; Brooks, A I; Dean, G E; Standifer, K M; Pasternak, G W (1995. ) Cloning and functional characterization through antisense mapping of a kappa 3-related opioid receptor. Molecular pharmacology, , 47 (6 ) ,1180-8

Perler, F B; Davis, E O; Dean, G E; Gimble, F S; Jack, W E; Neff, N; Noren, C J; Thorner, J; Belfort, M (1994. ) Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature. Nucleic acids research, , 22 (7 ) ,1125-7

Howell, M; Shirvan, A; Stern-Bach, Y; Steiner-Mordoch, S; Strasser, J E; Dean, G E; Schuldiner, S (1994. ) Cloning and functional expression of a tetrabenazine sensitive vesicular monoamine transporter from bovine chromaffin granules. FEBS letters, , 338 (1 ) ,16-22

Zemlan, F P; Vogelsang, G D; McLaughlin, L; Dean, G E (1994. ) Alzheimer's paired helical filaments: amyloid precursor protein epitope mapping. Brain research bulletin, , 33 (4 ) ,387-92

Schafer, M P; Dean, G E (1993. ) Cloning and sequence analysis of an H(+)-ATPase-encoding gene from the human dimorphic pathogen Histoplasma capsulatum. Gene, , 136 (1-2 ) ,295-300

Pan, Y X; Gu, H H; Xu, J; Dean, G E (1993. ) Saccharomyces cerevisiae expression of exogenous vacuolar ATPase subunits B. Biochimica et biophysica acta, , 1151 (2 ) ,175-85

Gu, H H; Xu, J; Gallagher, M; Dean, G E (1993. ) Peptide splicing in the vacuolar ATPase subunit A from Candida tropicalis. The Journal of biological chemistry, , 268 (10 ) ,7372-81

Pan, Y X; Xu, J; Strasser, J E; Howell, M; Dean, G E (1991. ) Structure and expression of subunit A from the bovine chromaffin cell vacuolar ATPase. FEBS letters, , 293 (1-2 ) ,89-92

Gu, H H; Gallagher, M J; Rupkey, S; Dean, G E (1990. ) Gene and derived peptide sequences for C. tropicalis vacuolar ATPase subunit b. Nucleic acids research, , 18 (24 ) ,7446

Vogelsang, G D; Zemlan, F P; Dean, G E (1990. ) Purification and solubilization of paired helical filaments from Alzheimer brains. Journal of neurochemistry, , 54 (1 ) ,148-55

Vogelsang, G D; Zemlan, F P; Dean, G E (1989. ) Hyperpurification of paired helical filaments reveals elevations in hydroxyproline content and a core structure related peptide fragment. Progress in clinical and biological research, , 317 ,791-800

Cuppoletti, J; Strasser, J E; Dean, G E (1988. ) An immunoreactive 8-azido ATP-labeled protein common to the lysosomal and chromaffin granule membrane. Biochimica et biophysica acta, , 946 (1 ) ,33-9

Galloway, C J; Dean, G E; Fuchs, R; Mellman, I (1988. ) Analysis of endosome and lysosome acidification in vitro. Methods in enzymology, , 157 ,601-11

Homma, M; Aizawa, S; Dean, G E; Macnab, R M (1987. ) Identification of the M-ring protein of the flagellar motor of Salmonella typhimurium. Proceedings of the National Academy of Sciences of the United States of America, , 84 (21 ) ,7483-7

Dean, G E; Nelson, P J; Agnew, W S; Rudnick, G (1987. ) Hydrodynamic properties of the chromaffin granule hydrogen ion pumping adenosinetriphosphatase. Biochemistry, , 26 (8 ) ,2301-5

Dean, G E; Nelson, P J; Rudnick, G (1986. ) Characterization of native and reconstituted hydrogen ion pumping adenosinetriphosphatase of chromaffin granules. Biochemistry, , 25 (17 ) ,4918-25

Marsh, M; Griffiths, G; Dean, G E; Mellman, I; Helenius, A (1986. ) Three-dimensional structure of endosomes in BHK-21 cells. Proceedings of the National Academy of Sciences of the United States of America, , 83 (9 ) ,2899-903

Stader, J; Matsumura, P; Vacante, D; Dean, G E; Macnab, R M (1986. ) Nucleotide sequence of the Escherichia coli motB gene and site-limited incorporation of its product into the cytoplasmic membrane. Journal of bacteriology, , 166 (1 ) ,244-52

Aizawa, S I; Dean, G E; Jones, C J; Macnab, R M; Yamaguchi, S (1985. ) Purification and characterization of the flagellar hook-basal body complex of Salmonella typhimurium. Journal of bacteriology, , 161 (3 ) ,836-49

Rudnick, G; Humphreys, C J; Dean, G E (1985. ) Serotonin transport by platelet plasma and granule membranes. Annals of the New York Academy of Sciences, , 456 ,277-8

Dean, G E; Macnab, R M; Stader, J; Matsumura, P; Burks, C (1984. ) Gene sequence and predicted amino acid sequence of the motA protein, a membrane-associated protein required for flagellar rotation in Escherichia coli. Journal of bacteriology, , 159 (3 ) ,991-9

Dean, G E; Fishkes, H; Nelson, P J; Rudnick, G (1984. ) The hydrogen ion-pumping adenosine triphosphatase of platelet dense granule membrane. Differences from F1F0- and phosphoenzyme-type ATPases. The Journal of biological chemistry, , 259 (15 ) ,9569-74

Nelson, P J; Dean, G E; Aronson, P S; Rudnick, G (1983. ) Hydrogen ion cotransport by the renal brush border glutamate transporter. Biochemistry, , 22 (23 ) ,5459-63

Galloway, C J; Dean, G E; Marsh, M; Rudnick, G; Mellman, I (1983. ) Acidification of macrophage and fibroblast endocytic vesicles in vitro. Proceedings of the National Academy of Sciences of the United States of America, , 80 (11 ) ,3334-8

Dean, G E; Aizawa, S I; Macnab, R M (1983. ) flaAII (motC, cheV) of Salmonella typhimurium is a structural gene involved in energization and switching of the flagellar motor. Journal of bacteriology, , 154 (1 ) ,84-91

Bowling, B.V., C.A. Huether, L. Wang, M.F. Myers, G.C. Markle, G.E. Dean, E.E. Acra, F.P. Wray, G.A. Jacob (2008. ) Genetic Literacy of Undergraduate Non-Science Majors and the Impact of Introductory Biology and Genetics Courses .BioScience, , 58 (7 ) ,654

Weingart, C., Broitman-Maduro, G., Dean, G., Newman, S., Peppler, M., and Weiss, A. (1999. ) Effect of labeling treatments on phagocytosis of Bordatella pertussis by human neutrophils .Infection & Immunity, , 67 (8 ) ,4264

Su, Shengchang; Bangar, Hansraj; Saldanha, Roland; Pemberton, Adin; Aronow, Bruce; Dean, Gary E; Lamkin, Thomas J; Hassett, Daniel J (2014. ) Construction and characterization of stable, constitutively expressed, chromosomal green and red fluorescent transcriptional fusions in the select agents, Bacillus anthracis, Yersinia pestis, Burkholderia mallei, and Burkholderia pseudomallei.MicrobiologyOpen, , 3 (5 ) ,610-29

Post Graduate Training and Education

1983-1985 Postdoctoral Fellow, Yale University School of Medicine, Pharmacology, , New Haven, CT

Keywords

Molecular Biologist,Biochemist,Molecular Geneticist,Cell Biologist,Energetics,Molecular Interaction,Protein Purification,Protein Synthesis,Nucleic Acid Synthesis,Cell Organelles,Cell Substructures,Lysosomes,Membranes,Membrane Transport,Phagocytosis,Endocrine System,Nervous System,Fungal Physiology,Vertebrate Endocrine System,Adrenal Gland,Science Education

Contact Information

Academic - Dept. Molecular Genetics, Biochemistry & Microbiology
231 Albert Sabin Way
Cincinnati  Ohio, 45267-0524
Phone: 513-558-0065
Fax: 513-558-0014
gary.dean@uc.edu