Jiajie Diao, PhD

Jiajie Diao, PhD

Asst Professor

Vontz Center

3308

COM Can Bio Diao Lab - 0521

Vontz Center for Molecular Studies
3125 Eden Avenue
Cincinnati, Ohio 45267

Education

Doctoral Degree, University of Illinois Urbana-Champaign, (Physics)

Post Doctoral Fellowship, Stanford University & HHMI

Research Support

Grant: #R35GM128837 Investigators:Diao, Jiajie 08-01-2018 -07-31-2023 National Institute of General Medical Sciences SNARE-mediated membrane fusion involved in autophagosome biogenesis Role:PI $364,923.00 Active Level:Federal

Grant: #16661 Investigators:Diao, Jiajie; Seroogy, Kim 11-01-2018 -04-30-2020 Michael J. Fox Foundation for Parkinson's Research Chaperones of alpha-synuclein for membrane fusion involved in synaptic transmission Role:PI $99,081.00 Awarded Level:Private Non-Profit

Grant: #R21AG061600 Investigators:Diao, Jiajie 09-30-2019 -06-30-2021 National Institute on Aging Single-molecule study of alpha-synuclein Role:PI $200,625.00 Awarded Level:Federal

Publications

Peer Reviewed Publications

Huang, Huaiyi; Yang, Liang; Zhang, Pingyu; Qiu, Kangqiang; Huang, Juanjuan; Chen, Yu; Diao, JiaJie; Liu, Jiankang; Ji, Liangnian; Long, Jiangang; Chao, Hui (2016. )Real-time tracking mitochondrial dynamic remodeling with two-photon phosphorescent iridium (III) complexes.Biomaterials, ,83 ,321-31

Hu, Rui; Diao, Jiajie; Li, Ji; Tang, Zhipeng; Li, Xiaoqing; Leitz, Jeremy; Long, Jiangang; Liu, Jiankang; Yu, Dapeng; Zhao, Qing (2016. )Intrinsic and membrane-facilitated alpha-synuclein oligomerization revealed by label-free detection through solid-state nanopores.Scientific reports, ,6 ,20776

Diao, Jiajie; Grob, Patricia; Cipriano, Daniel J; Kyoung, Minjoung; Zhang, Yunxiang; Shah, Sachi; Nguyen, Amie; Padolina, Mark; Srivastava, Ankita; Vrljic, Marija; Shah, Ankita; Nogales, Eva; Chu, Steven; Brunger, Axel T (2015. )Correction: Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion.eLife, ,4 ,e12289

Zhou, Qiangjun; Lai, Ying; Bacaj, Taulant; Zhao, Minglei; Lyubimov, Artem Y; Uervirojnangkoorn, Monarin; Zeldin, Oliver B; Brewster, Aaron S; Sauter, Nicholas K; Cohen, Aina E; Soltis, S Michael; Alonso-Mori, Roberto; Chollet, Matthieu; Lemke, Henrik T; Pfuetzner, Richard A; Choi, Ucheor B; Weis, William I; Diao, Jiajie; Südhof, Thomas C; Brunger, Axel T (2015. )Architecture of the synaptotagmin-SNARE machinery for neuronal exocytosis.Nature, ,525 (7567 ),62-7

Diao, Jiajie; Liu, Rong; Rong, Yueguang; Zhao, Minglei; Zhang, Jing; Lai, Ying; Zhou, Qiangjun; Wilz, Livia M; Li, Jianxu; Vivona, Sandro; Pfuetzner, Richard A; Brunger, Axel T; Zhong, Qing (2015. )ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes.Nature, ,520 (7548 ),563-6

Lai, Ying; Zhao, Lin; Bu, Bing; Lou, Xiaochu; Li, Dechang; Ji, Baohua; Liu, Jiankang; Diao, Jiajie; Shin, Yeon-Kyun (2015. )Lipid molecules influence early stages of yeast SNARE-mediated membrane fusion.Physical biology, ,12 (2 ),025003

Zhang, Yunxiang; Diao, Jiajie; Colbert, Karen N; Lai, Ying; Pfuetzner, Richard A; Padolina, Mark S; Vivona, Sandro; Ressl, Susanne; Cipriano, Daniel J; Choi, Ucheor B; Shah, Niket; Weis, William I; Brunger, Axel T (2015. )Munc18a does not alter fusion rates mediated by neuronal SNAREs, synaptotagmin, and complexin.The Journal of biological chemistry, ,290 (16 ),10518-34

Lai, Ying; Lou, Xiaochu; Diao, Jiajie; Shin, Yeon-Kyun (2015. )Molecular origins of synaptotagmin 1 activities on vesicle docking and fusion pore opening.Scientific reports, ,5 ,9267

Brunger, Axel T; Cipriano, Daniel J; Diao, Jiajie (2015. )Towards reconstitution of membrane fusion mediated by SNAREs and other synaptic proteins.Critical reviews in biochemistry and molecular biology, ,50 (3 ),231-41

Lai, Ying; Diao, Jiajie; Cipriano, Daniel J; Zhang, Yunxiang; Pfuetzner, Richard A; Padolina, Mark S; Brunger, Axel T (2014. )Complexin inhibits spontaneous release and synchronizes Ca2+-triggered synaptic vesicle fusion by distinct mechanisms. eLife, ,3 ,e03756

Lai, Ying; Kim, Sunae; Varkey, Jobin; Lou, Xiaochu; Song, Jae-Kyun; Diao, Jiajie; Langen, Ralf; Shin, Yeon-Kyun (2014. )Nonaggregated alpha-synuclein influences SNARE-dependent vesicle docking via membrane binding.Biochemistry, ,53 (24 ),3889-96

Diao, Jiajie; Cipriano, Daniel J; Zhao, Minglei; Zhang, Yunxiang; Shah, Sachi; Padolina, Mark S; Pfuetzner, Richard A; Brunger, Axel T (2013. )Complexin-1 enhances the on-rate of vesicle docking via simultaneous SNARE and membrane interactions.Journal of the American Chemical Society, ,135 (41 ),15274-7

Diao, Jiajie; Zhao, Minglei; Zhang, Yunxiang; Kyoung, Minjoung; Brunger, Axel T (2013. )Studying protein-reconstituted proteoliposome fusion with content indicators in vitro.BioEssays : news and reviews in molecular, cellular and developmental biology, ,35 (7 ),658-65

Burré, Jacqueline; Vivona, Sandro; Diao, Jiajie; Sharma, Manu; Brunger, Axel T; Südhof, Thomas C (2013. )Properties of native brain alpha-synuclein.Nature, ,498 (7453 ),E4-6; discussion E6-7

Diao, Jiajie; Burré, Jacqueline; Vivona, Sandro; Cipriano, Daniel J; Sharma, Manu; Kyoung, Minjoung; Südhof, Thomas C; Brunger, Axel T (2013. )Native alpha-synuclein induces clustering of synaptic-vesicle mimics via binding to phospholipids and synaptobrevin-2/VAMP2.eLife, ,2 ,e00592

Lai, Ying; Diao, Jiajie; Liu, Yanxin; Ishitsuka, Yuji; Su, Zengliu; Schulten, Klaus; Ha, Taekjip; Shin, Yeon-Kyun (2013. )Fusion pore formation and expansion induced by Ca2+ and synaptotagmin 1.Proceedings of the National Academy of Sciences of the United States of America, ,110 (4 ),1333-8

Kyoung, Minjoung; Zhang, Yunxiang; Diao, Jiajie; Chu, Steven; Brunger, Axel T (2013. )Studying calcium-triggered vesicle fusion in a single vesicle-vesicle content and lipid-mixing system.Nature protocols, ,8 (1 ),1-16

Diao, Jiajie; Ishitsuka, Yuji; Lee, Hanki; Joo, Chirlmin; Su, Zengliu; Syed, Salman; Shin, Yeon-Kyun; Yoon, Tae-Young; Ha, Taekjip (2012. )A single vesicle-vesicle fusion assay for in vitro studies of SNAREs and accessory proteins. Nature protocols, ,7 (5 ),921-34

Diao, Jiajie; Ishitsuka, Yuji; Bae, Woo-Ri (2011. )Single-molecule FRET study of SNARE-mediated membrane fusion.Bioscience reports, ,31 (6 ),457-63

Kyoung, Minjoung; Srivastava, Ankita; Zhang, Yunxiang; Diao, Jiajie; Vrljic, Marija; Grob, Patricia; Nogales, Eva; Chu, Steven; Brunger, Axel T (2011. )In vitro system capable of differentiating fast Ca2+-triggered content mixing from lipid exchange for mechanistic studies of neurotransmitter release.Proceedings of the National Academy of Sciences of the United States of America, ,108 (29 ),E304-13

Diao, Jiajie; Su, Zengliu; Ishitsuka, Yuji; Lu, Bin; Lee, Kyung Suk; Lai, Ying; Shin, Yeon-Kyun; Ha, Taekjip (2010. )A single-vesicle content mixing assay for SNARE-mediated membrane fusion.Nature communications, ,1 ,54

Diao, Jiajie; Su, Zengliu; Lu, Xiaobing; Yoon, Tae-Young; Shin, Yeon-Kyun; Ha, Taekjip (2010. )Single-Vesicle Fusion Assay Reveals Munc18-1 Binding to the SNARE Core Is Sufficient for Stimulating Membrane Fusion.ACS chemical neuroscience, ,1 (3 ),168-174

Diao, Jiajie; Yoon, Tae-Young; Su, Zengliu; Shin, Yeon-Kyun; Ha, Taekjip (2009. )C2AB: a molecular glue for lipid vesicles with a negatively charged surface.Langmuir : the ACS journal of surfaces and colloids, ,25 (13 ),7177-80

Yoon, Tae-Young; Lu, Xiaobing; Diao, Jiajie; Lee, Soo-Min; Ha, Taekjip; Shin, Yeon-Kyun (2008. )Complexin and Ca2+ stimulate SNARE-mediated membrane fusion.Nature structural & molecular biology, ,15 (7 ),707-13

Song, Chun-Xiao; Diao, Jiajie; Brunger, Axel T; Quake, Stephen R (2016. )Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation.Proceedings of the National Academy of Sciences of the United States of America, ,113 (16 ),4338-43

Diao, Jiajie; Grob, Patricia; Cipriano, Daniel J; Kyoung, Minjoung; Zhang, Yunxiang; Shah, Sachi; Nguyen, Amie; Padolina, Mark; Srivastava, Ankita; Vrljic, Marija; Shah, Ankita; Nogales, Eva; Chu, Steven; Brunger, Axel T (2012. )Synaptic proteins promote calcium-triggered fast transition from point contact to full fusion.eLife, ,1 ,e00109

Malmersjö, Seth; Di Palma, Serena; Diao, Jiajie; Lai, Ying; Pfuetzner, Richard A; Wang, Austin L; McMahon, Moira A; Hayer, Arnold; Porteus, Matthew; Bodenmiller, Bernd; Brunger, Axel T; Meyer, Tobias (2016. )Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion.The EMBO journal, ,

Wang, Chuchu; Zhao, Chunyu; Li, Dan; Tian, Zhiqi; Lai, Ying; Diao, Jiajie; Liu, Cong (2016. )Versatile Structures of α-Synuclein.Frontiers in molecular neuroscience, ,9 ,48

Qing, Ying; Tian, Zhiqi; Bi, Ying; Wang, Yongyao; Long, Jiangang; Song, Chun-Xiao; Diao, Jiajie (2017. )Quantitation and mapping of the epigenetic marker 5-hydroxymethylcytosine.BioEssays : news and reviews in molecular, cellular and developmental biology, ,39 (5 ),

Wang, Yongyao; Li, Linsen; Hou, Chen; Lai, Ying; Long, Jiangang; Liu, Jiankang; Zhong, Qing; Diao, Jiajie (2016. )SNARE-mediated membrane fusion in autophagy.Seminars in cell & developmental biology, ,60 ,97-104

Gong, Jihong; Lai, Ying; Li, Xiaohong; Wang, Mengxian; Leitz, Jeremy; Hu, Yachong; Zhang, Yunxiang; Choi, Ucheor B; Cipriano, Daniel; Pfuetzner, Richard A; Südhof, Thomas C; Yang, Xiaofei; Brunger, Axel T; Diao, Jiajie (2016. )C-terminal domain of mammalian complexin-1 localizes to highly curved membranes.Proceedings of the National Academy of Sciences of the United States of America, ,113 (47 ),E7590-E7599

Lai, Ying; Choi, Ucheor B; Zhang, Yunxiang; Zhao, Minglei; Pfuetzner, Richard A; Wang, Austin L; Diao, Jiajie; Brunger, Axel T (2016. )N-terminal domain of complexin independently activates calcium-triggered fusion.Proceedings of the National Academy of Sciences of the United States of America, ,113 (32 ),E4698-707

Z. Tian, Y. Hu, J. Long, J. Liu, J. Diao (2016. )Single-molecule biophysical studies of neurotransmitter release.Prog. Biochem. Biophys, ,43 ,946

X. Li, R. Hu, J. Li, X. Tong, J.J. Diao, D. Yu, Q. Zhao (2016. )Non-sticky translocation of bio-molecules through Tween 20-coated solid-state nanopores in a wide pH range.Appl. Phys. Lett, ,109 ,143105

J. Diao, L. Li, Y. Lai, Q. Zhong (2017. )In vitro reconstitution of autophagosome-lysosome fusion.Methods Enzymo, ,586 ,365-376

B. Bu, D. Li, J. Diao, B. Ji (2017. )Mechanics of water pore formation in lipid membrane under electric field.Acta Mech. Sin, ,33 (2 ),23242

Y. Hu, Y. Lai, Y. Wang, M. Zhao, Y. Zhang, M. Crowe, Z. Tian, J. Long, J. Diao (2017. )SNARE-reconstituted liposomes as controllable zeptoliter nanoreactors for macromolecules.Adv. Biosys, ,1 ,

S.C. Shen, W. Liu, J.J. Diao (2015. )Nanoparticle wire structures for biophysical detections.Chin. Phys, ,B 24 ,127308

L. Shao, J.J. Diao, Z. Tang, S. Liu, S.C. Shen, X. Rui, J. Liu, D. Yu, Q. Zhao (2014. )Gold nanoparticle wires for sensing DNA and DNA/protein interactions.Nanoscale, ,6 ,4089

Song, Chun-Xiao; Yin, Senlin; Ma, Li; Wheeler, Amanda; Chen, Yu; Zhang, Yan; Liu, Bin; Xiong, Junjie; Zhang, Weihan; Hu, Jiankun; Zhou, Zongguang; Dong, Biao; Tian, Zhiqi; Jeffrey, Stefanie S; Chua, Mei-Sze; So, Samuel; Li, Weimin; Wei, Yuquan; Diao, Jiajie; Xie, Dan; Quake, Stephen R (2017. )5-Hydroxymethylcytosine signatures in cell-free DNA provide information about tumor types and stages.Cell research, ,

Bu, Bing; Tong, Xin; Li, Dechang; Hu, Yachong; He, Wangxiao; Zhao, Chunyu; Hu, Rui; Li, Xiaoqing; Shao, Yongping; Liu, Cong; Zhao, Qing; Ji, Baohua; Diao, Jiajie (2017. )N-Terminal Acetylation Preserves α-Synuclein from Oligomerization by Blocking Intermolecular Hydrogen Bonds.ACS chemical neuroscience, ,

Jiang, Xi; Hu, Chao; Ferchen, Kyle; Nie, Ji; Cui, Xiaolong; Chen, Chih-Hong; Cheng, Liting; Zuo, Zhixiang; Seibel, William; He, Chunjiang; Tang, Yixuan; Skibbe, Jennifer R; Wunderlich, Mark; Reinhold, William C; Dong, Lei; Shen, Chao; Arnovitz, Stephen; Ulrich, Bryan; Lu, Jiuwei; Weng, Hengyou; Su, Rui; Huang, Huilin; Wang, Yungui; Li, Chenying; Qin, Xi; Mulloy, James; Zheng, Yi; Diao, Jiajie; Jin, Jie; Li, Chong; Liu, Paul P; He, Chuan; Chen, Yuan; Chen, Jianjun (2017. )Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia.Nature communications, ,8 (1 ),2099

Bhattacharjee, Anukana; Wang, Yongyao; Diao, Jiajie; Price, Carolyn M (2017. )Dynamic DNA binding, junction recognition and G4 melting activity underlie the telomeric and genome-wide roles of human CST.Nucleic acids research, ,45 (21 ),12311-12324

Qiu, Kangqiang; Ke, Libing; Zhang, Xuepeng; Liu, Yukang; Rees, Thomas W; Ji, Liangnian; Diao, Jiajie; Chao, Hui (2018. )Tracking mitochondrial pH fluctuation during cell apoptosis with two-photon phosphorescent iridium(iii) complexes.Chemical communications (Cambridge, England), ,54 (19 ),2421-2424

Bu, Bing; Crowe, Michael; Diao, Jiajie; Ji, Baohua; Li, Dechang (2018. )Cholesterol suppresses membrane leakage by decreasing water penetrability.Soft matter, ,14 (25 ),5277-5282

Jiang, Xi; Hu, Chao; Ferchen, Kyle; Nie, Ji; Cui, Xiaolong; Chen, Chih-Hong; Cheng, Liting; Zuo, Zhixiang; Seibel, William; He, Chunjiang; Tang, Yixuan; Skibbe, Jennifer R; Wunderlich, Mark; Reinhold, William C; Dong, Lei; Shen, Chao; Arnovitz, Stephen; Ulrich, Bryan; Lu, Jiuwei; Weng, Hengyou; Su, Rui; Huang, Huilin; Wang, Yungui; Li, Chenying; Qin, Xi; Mulloy, James C; Zheng, Yi; Diao, Jiajie; Jin, Jie; Li, Chong; Liu, Paul P; He, Chuan; Chen, Yuan; Chen, Jianjun (2018. )Author Correction: Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia.Nature communications, ,9 (1 ),670

Hu, Yachong; Tian, Zhiqi; Diao, Jiajie (2019. )Single-Molecule Fluorescence Measurement of SNARE-Mediated Vesicle Fusion.Methods in molecular biology (Clifton, N.J.), ,1860 ,335-344

Khamo, John S; Krishnamurthy, Vishnu V; Chen, Qixin; Diao, Jiajie; Zhang, Kai (2018. )Optogenetic Delineation of Receptor Tyrosine Kinase Subcircuits in PC12 Cell Differentiation.Cell chemical biology, ,

Chen, Qixin; Jin, Chengzhi; Shao, Xintian; Guan, Ruilin; Tian, Zhiqi; Wang, Chenran; Liu, Fei; Ling, Peixue; Guan, Jun-Lin; Ji, Liangnian; Wang, Fengshan; Chao, Hui; Diao, Jiajie (2018. )Super-Resolution Tracking of Mitochondrial Dynamics with An Iridium(III) Luminophore.Small (Weinheim an der Bergstrasse, Germany), ,14 (41 ),e1802166

Leikina, Evgenia; Gamage, Dilani G; Prasad, Vikram; Goykhberg, Joanna; Crowe, Michael; Diao, Jiajie; Kozlov, Michael M; Chernomordik, Leonid V; Millay, Douglas P (2018. )Myomaker and Myomerger Work Independently to Control Distinct Steps of Membrane Remodeling during Myoblast Fusion.Developmental cell, ,46 (6 ),767-780.e7

Feng, Xuyang; Hsu, Shih-Jui; Bhattacharjee, Anukana; Wang, Yongyao; Diao, Jiajie; Price, Carolyn M (2018. )CTC1-STN1 terminates telomerase while STN1-TEN1 enables C-strand synthesis during telomere replication in colon cancer cells.Nature communications, ,9 (1 ),2827

Tian, Zhiqi; Gong, Jihong; Crowe, Michael; Lei, Ming; Li, Dechang; Ji, Baohua; Diao, Jiajie (2019. )Biochemical studies of membrane fusion at the single-particle level.Progress in lipid research, ,73 ,92-100

Cai, Bin; Yu, Luning; Sharum, Savanna R; Zhang, Kai; Diao, Jiajie (2019. )Single-vesicle measurement of protein-induced membrane tethering.Colloids and surfaces. B, Biointerfaces, ,177 ,267-273

Ma, Dong-Fei; Xu, Chun-Hua; Hou, Wen-Qing; Zhao, Chun-Yu; Ma, Jian-Bing; Huang, Xing-Yuan; Jia, Qi; Ma, Lu; Diao, Jiajie; Liu, Cong; Li, Ming; Lu, Ying (2019. )Detecting Single-Molecule Dynamics on Lipid Membranes with Quenchers-in-a-Liposome FRET.Angewandte Chemie (International ed. in English), ,

Diao, J; Li, L; Lai, Y; Zhong, Q (2017. )In Vitro Reconstitution of Autophagosome-Lysosome Fusion.Methods in enzymology, ,587 ,365-376

Brose, Nils; Brunger, Axel; Cafiso, David; Chapman, Edwin R; Diao, Jiajie; Hughson, Frederick M; Jackson, Meyer B; Jahn, Reinhard; Lindau, Manfred; Ma, Cong; Rizo, Josep; Shin, Yeon-Kyun; Söllner, Thomas H; Tamm, Lukas; Yoon, Tae-Young; Zhang, Yongli (2019. )Synaptic vesicle fusion: today and beyond.Nature structural & molecular biology, ,26 (8 ),663-668

Jiang, Xi; Hu, Chao; Ferchen, Kyle; Nie, Ji; Cui, Xiaolong; Chen, Chih-Hong; Cheng, Liting; Zuo, Zhixiang; Seibel, William; He, Chunjiang; Tang, Yixuan; Skibbe, Jennifer R; Wunderlich, Mark; Reinhold, William C; Dong, Lei; Shen, Chao; Arnovitz, Stephen; Ulrich, Bryan; Lu, Jiuwei; Weng, Hengyou; Su, Rui; Huang, Huilin; Wang, Yungui; Li, Chenying; Qin, Xi; Mulloy, James C; Zheng, Yi; Diao, Jiajie; Jin, Jie; Li, Chong; Liu, Paul P; He, Chuan; Chen, Yuan; Chen, Jianjun (2017. )Targeted inhibition of STAT/TET1 axis as a therapeutic strategy for acute myeloid leukemia.Nature communications, ,8 (1 ),2099

Bhattacharjee, Anukana; Wang, Yongyao; Diao, Jiajie; Price, Carolyn M (2017. )Dynamic DNA binding, junction recognition and G4 melting activity underlie the telomeric and genome-wide roles of human CST.Nucleic acids research, ,45 (21 ),12311-12324

Positions and Work Experience

- Our group has an interest in Membrane Biophysics (Single-vesicle study of SNARE-mediated membrane fusion; Single-molecule analysis of protein conformational change in membrane environment (membrane property change induced by protein) and Nanotechnology (nanoparticle structures for biomedical detection).,

Research and Practice Interests

Our group has an interest in Membrane Biophysics (Single-vesicle study of SNARE-mediated membrane fusion; Single-molecule analysis of protein conformational change in membrane environment (membrane property change induced by protein) and Nanotechnology (nanoparticle structures for biomedical detection).

Contact Information

Academic - Vontz Center for Molecular Studies
3125 Eden Avenue
Cincinnati  Ohio, 45267
Phone: 513-558-0234
jiajie.diao@uc.edu