Brian Gebelein

Associate Professor - Aff

Childrens Hospital Bldg R

COM Pediatrics Dev. Biology - 0054

CCHMC S Building

Education

B.S., University of Wisconsin Milwaukee, WI,, 1994

Ph.D., Mayo Graduate School Rochester, MN, 2000

Research and Practice Interests

The human body is composed of an estimated 50 trillion cells that can be classified into
approximately 210 different cell types. Altogether, these cells form organ systems that perform specialized functions required for digestion, respiration, immunity, locomotion, sensory detection, and decision-making. Amazingly, each cell type arises from a common fertilized egg, and with very rare exceptions, contains an identical set of genomic instructions. Unfortunately, our lack of understanding of how the genome is differentially utilized to direct the formation of each cell type currently limits our ability to treat a variety of human maladies including human birth defects. My laboratory is focused on understanding how transcription factors regulate the expression of target genes to specify distinct cells, tissues, and organs along the body plan of the fruit-fly Drosophila melanogaster. Since the genetic pathways regulating development are highly conserved between flies and mammals, we can take advantage of the powerful genetic approaches available in Drosophila to uncover new insights into human development. Overall, my goals are to obtain a high-resolution understanding of how conserved transcription factors are integrated to yield cell-specific gene expression during fly development and to translate these findings into mammalian systems through collaborations with other laboratories here at Cincinnati Children’s Hospital and the University of Cincinnati College of Medicine.

Publications

Peer Reviewed Publications

Bryantsev, Anton L; Duong, Sandy; Brunetti, Tonya M; Chechenova, Maria B; Lovato, TyAnna L; Nelson, Cloyce; Shaw, Elizabeth; Uhl, Juli D; Gebelein, Brian; Cripps, Richard M (2012. ) Extradenticle and homothorax control adult muscle fiber identity in Drosophila.Developmental cell, , 23 (3 ) ,664-73

Fujioka, Miki; Gebelein, Brian; Cofer, Zenobia C; Mann, Richard S; Jaynes, James B (2012. ) Engrailed cooperates directly with Extradenticle and Homothorax on a distinct class of homeodomain binding sites to repress sloppy paired.Developmental biology, , 366 (2 ) ,382-92

Li-Kroeger, David; Cook, Tiffany A; Gebelein, Brian (2012. ) Integration of an abdominal Hox complex with Pax2 yields cell-specific EGF secretion from Drosophila sensory precursor cells.Development (Cambridge, England), , 139 (9 ) ,1611-9

Burns, Kevin A; Gutzwiller, Lisa M; Tomoyasu, Yoshinori; Gebelein, Brian (2012. ) Oenocyte development in the red flour beetle Tribolium castaneum.Development genes and evolution, , 222 (2 ) ,77-88

Terrell, David; Xie, Baotong; Workman, Michael; Mahato, Simpla; Zelhof, Andrew; Gebelein, Brian; Cook, Tiffany (2012. ) OTX2 and CRX rescue overlapping and photoreceptor-specific functions in the Drosophila eye.Developmental dynamics : an official publication of the American Association of Anatomists, , 241 (1 ) ,215-28

Johnston, Robert J; Otake, Yoshiaki; Sood, Pranidhi; Vogt, Nina; Behnia, Rudy; Vasiliauskas, Daniel; McDonald, Elizabeth; Xie, Baotong; Koenig, Sebastian; Wolf, Reinhard; Cook, Tiffany; Gebelein, Brian; Kussell, Edo; Nakagoshi, Hideki; Desplan, Claude (2011. ) Interlocked feedforward loops control cell-type-specific Rhodopsin expression in the Drosophila eye.Cell, , 145 (6 ) ,956-68

Charlton-Perkins, Mark; Whitaker, S Leigh; Fei, Yueyang; Xie, Baotong; Li-Kroeger, David; Gebelein, Brian; Cook, Tiffany (2011. ) Prospero and Pax2 combinatorially control neural cell fate decisions by modulating Ras- and Notch-dependent signaling.Neural development, , 6 ,20

Gutzwiller, Lisa M; Witt, Lorraine M; Gresser, Amy L; Burns, Kevin A; Cook, Tiffany A; Gebelein, Brian (2010. ) Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen.Developmental biology, , 348 (2 ) ,231-43

McDonald, Elizabeth C; Xie, Baotong; Workman, Michael; Charlton-Perkins, Mark; Terrell, David A; Reischl, Joachim; Wimmer, Ernst A; Gebelein, Brian A; Cook, Tiffany A (2010. ) Separable transcriptional regulatory domains within Otd control photoreceptor terminal differentiation events.Developmental biology, , 347 (1 ) ,122-32

Witt, Lorraine M; Gutzwiller, Lisa M; Gresser, Amy L; Li-Kroeger, David; Cook, Tiffany A; Gebelein, Brian (2010. ) Atonal, Senseless, and Abdominal-A regulate rhomboid enhancer activity in abdominal sensory organ precursors.Developmental biology, , 344 (2 ) ,1060-70

Phelan, James D; Shroyer, Noah F; Cook, Tiffany; Gebelein, Brian; Grimes, H Leighton (2010. ) Gfi1-cells and circuits: unraveling transcriptional networks of development and disease.Current opinion in hematology, , 17 (4 ) ,300-7

Uhl, Juli D; Cook, Tiffany A; Gebelein, Brian (2010. ) Comparing anterior and posterior Hox complex formation reveals guidelines for predicting cis-regulatory elements.Developmental biology, , 343 (1-2 ) ,154-66

Lomberk, Gwen A; Imoto, Issei; Gebelein, Brian; Urrutia, Raul; Cook, Tiffany A (2010. ) Conservation of the TGFbeta/Labial homeobox signaling loop in endoderm-derived cells between Drosophila and mammals.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], , 10 (1 ) ,74-84

Horman, Shane R; Velu, Chinavenmeni S; Chaubey, Aditya; Bourdeau, Tristan; Zhu, Jinfang; Paul, William E; Gebelein, Brian; Grimes, H Leighton (2009. ) Gfi1 integrates progenitor versus granulocytic transcriptional programming.Blood, , 113 (22 ) ,5466-75

Gebelein, Brian The control of EGF signaling and cell fate in the Drosophila abdomen.Fly, , 2 (5 ) ,257-8

Li-Kroeger, David; Witt, Lorraine M; Grimes, H Leighton; Cook, Tiffany A; Gebelein, Brian (2008. ) Hox and senseless antagonism functions as a molecular switch to regulate EGF secretion in the Drosophila PNS.Developmental cell, , 15 (2 ) ,298-308

Xie, Baotong; Charlton-Perkins, Mark; McDonald, Elizabeth; Gebelein, Brian; Cook, Tiffany (2007. ) Senseless functions as a molecular switch for color photoreceptor differentiation in Drosophila.Development (Cambridge, England), , 134 (23 ) ,4243-53

Gebelein, Brian; Mann, Richard S (2007. ) Compartmental modulation of abdominal Hox expression by engrailed and sloppy-paired patterns the fly ectoderm.Developmental biology, , 308 (2 ) ,593-605

Gebelein, Brian; McKay, Daniel J; Mann, Richard S (2004. ) Direct integration of Hox and segmentation gene inputs during Drosophila development.Nature, , 431 (7009 ) ,653-9

Gebelein, Brian; Culi, Joaquim; Ryoo, Hyung Don; Zhang, Wen; Mann, Richard S (2002. ) Specificity of Distalless repression and limb primordia development by abdominal Hox proteins. Developmental cell, , 3 (4 ) ,487-98

Bessa, Jose; Gebelein, Brian; Pichaud, Franck; Casares, Fernando; Mann, Richard S (2002. ) Combinatorial control of Drosophila eye development by eyeless, homothorax, and teashirt.Genes & development, , 16 (18 ) ,2415-27

Gresser, Amy L; Gutzwiller, Lisa M; Gauck, Mackenzie K; Hartenstein, Volker; Cook, Tiffany A; Gebelein, Brian (2015. ) Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability.PloS one, , 10 (8 ) ,e0134915

Velu, Chinavenmeni S; Chaubey, Aditya; Phelan, James D; Horman, Shane R; Wunderlich, Mark; Guzman, Monica L; Jegga, Anil G; Zeleznik-Le, Nancy J; Chen, Jianjun; Mulloy, James C; Cancelas, Jose A; Jordan, Craig T; Aronow, Bruce J; Marcucci, Guido; Bhat, Balkrishen; Gebelein, Brian; Grimes, H Leighton (2014. ) Therapeutic antagonists of microRNAs deplete leukemia-initiating cell activity.The Journal of clinical investigation, , 124 (1 ) ,222-36

Jukam, David; Xie, Baotong; Rister, Jens; Terrell, David; Charlton-Perkins, Mark; Pistillo, Daniela; Gebelein, Brian; Desplan, Claude; Cook, Tiffany (2013. ) Opposite feedbacks in the Hippo pathway for growth control and neural fate.Science (New York, N.Y.), , 342 (6155 ) ,1238016

Contact Information

CCHMC S Building
Phone: 513-636-3366
brian.gebelein@cchmc.org