Brian Gebelein , PhD

B.S.: University of Wisconsin Milwaukee, WI,, 1994

Ph.D.: Mayo Graduate School Rochester, MN, 2000

The human body is composed of an estimated 50 trillion cells that can be classified into
approximately 210 different cell types. Altogether, these cells form organ systems that perform specialized functions required for digestion, respiration, immunity, locomotion, sensory detection, and decision-making. Amazingly, each cell type arises from a common fertilized egg, and with very rare exceptions, contains an identical set of genomic instructions. Unfortunately, our lack of understanding of how the genome is differentially utilized to direct the formation of each cell type currently limits our ability to treat a variety of human maladies including human birth defects. My laboratory is focused on understanding how transcription factors regulate the expression of target genes to specify distinct cells, tissues, and organs along the body plan of the fruit-fly Drosophila melanogaster. Since the genetic pathways regulating development are highly conserved between flies and mammals, we can take advantage of the powerful genetic approaches available in Drosophila to uncover new insights into human development. Overall, my goals are to obtain a high-resolution understanding of how conserved transcription factors are integrated to yield cell-specific gene expression during fly development and to translate these findings into mammalian systems through collaborations with other laboratories here at Cincinnati Children’s Hospital and the University of Cincinnati College of Medicine.

Peer Reviewed Publications

Bryantsev, Anton L; Duong, Sandy; Brunetti, Tonya M; Chechenova, Maria B; Lovato, TyAnna L; Nelson, Cloyce; Shaw, Elizabeth; Uhl, Juli D; Gebelein, Brian; Cripps, Richard M (2012. ) Extradenticle and homothorax control adult muscle fiber identity in Drosophila.Developmental cell, , 23 (3 ) ,664-73 More Information

Fujioka, Miki; Gebelein, Brian; Cofer, Zenobia C; Mann, Richard S; Jaynes, James B (2012. ) Engrailed cooperates directly with Extradenticle and Homothorax on a distinct class of homeodomain binding sites to repress sloppy paired.Developmental biology, , 366 (2 ) ,382-92 More Information

Li-Kroeger, David; Cook, Tiffany A; Gebelein, Brian (2012. ) Integration of an abdominal Hox complex with Pax2 yields cell-specific EGF secretion from Drosophila sensory precursor cells.Development (Cambridge, England), , 139 (9 ) ,1611-9 More Information

Burns, Kevin A; Gutzwiller, Lisa M; Tomoyasu, Yoshinori; Gebelein, Brian (2012. ) Oenocyte development in the red flour beetle Tribolium castaneum.Development genes and evolution, , 222 (2 ) ,77-88 More Information

Terrell, David; Xie, Baotong; Workman, Michael; Mahato, Simpla; Zelhof, Andrew; Gebelein, Brian; Cook, Tiffany (2012. ) OTX2 and CRX rescue overlapping and photoreceptor-specific functions in the Drosophila eye.Developmental dynamics : an official publication of the American Association of Anatomists, , 241 (1 ) ,215-28 More Information

Johnston, Robert J; Otake, Yoshiaki; Sood, Pranidhi; Vogt, Nina; Behnia, Rudy; Vasiliauskas, Daniel; McDonald, Elizabeth; Xie, Baotong; Koenig, Sebastian; Wolf, Reinhard; Cook, Tiffany; Gebelein, Brian; Kussell, Edo; Nakagoshi, Hideki; Desplan, Claude (2011. ) Interlocked feedforward loops control cell-type-specific Rhodopsin expression in the Drosophila eye.Cell, , 145 (6 ) ,956-68 More Information

Charlton-Perkins, Mark; Whitaker, S Leigh; Fei, Yueyang; Xie, Baotong; Li-Kroeger, David; Gebelein, Brian; Cook, Tiffany (2011. ) Prospero and Pax2 combinatorially control neural cell fate decisions by modulating Ras- and Notch-dependent signaling.Neural development, , 6 ,20 More Information

Gutzwiller, Lisa M; Witt, Lorraine M; Gresser, Amy L; Burns, Kevin A; Cook, Tiffany A; Gebelein, Brian (2010. ) Proneural and abdominal Hox inputs synergize to promote sensory organ formation in the Drosophila abdomen.Developmental biology, , 348 (2 ) ,231-43 More Information

McDonald, Elizabeth C; Xie, Baotong; Workman, Michael; Charlton-Perkins, Mark; Terrell, David A; Reischl, Joachim; Wimmer, Ernst A; Gebelein, Brian A; Cook, Tiffany A (2010. ) Separable transcriptional regulatory domains within Otd control photoreceptor terminal differentiation events.Developmental biology, , 347 (1 ) ,122-32 More Information

Witt, Lorraine M; Gutzwiller, Lisa M; Gresser, Amy L; Li-Kroeger, David; Cook, Tiffany A; Gebelein, Brian (2010. ) Atonal, Senseless, and Abdominal-A regulate rhomboid enhancer activity in abdominal sensory organ precursors.Developmental biology, , 344 (2 ) ,1060-70 More Information

Phelan, James D; Shroyer, Noah F; Cook, Tiffany; Gebelein, Brian; Grimes, H Leighton (2010. ) Gfi1-cells and circuits: unraveling transcriptional networks of development and disease.Current opinion in hematology, , 17 (4 ) ,300-7 More Information

Uhl, Juli D; Cook, Tiffany A; Gebelein, Brian (2010. ) Comparing anterior and posterior Hox complex formation reveals guidelines for predicting cis-regulatory elements.Developmental biology, , 343 (1-2 ) ,154-66 More Information

Lomberk, Gwen A; Imoto, Issei; Gebelein, Brian; Urrutia, Raul; Cook, Tiffany A (2010. ) Conservation of the TGFbeta/Labial homeobox signaling loop in endoderm-derived cells between Drosophila and mammals.Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], , 10 (1 ) ,74-84 More Information

Horman, Shane R; Velu, Chinavenmeni S; Chaubey, Aditya; Bourdeau, Tristan; Zhu, Jinfang; Paul, William E; Gebelein, Brian; Grimes, H Leighton (2009. ) Gfi1 integrates progenitor versus granulocytic transcriptional programming.Blood, , 113 (22 ) ,5466-75 More Information

Gebelein, Brian The control of EGF signaling and cell fate in the Drosophila abdomen.Fly, , 2 (5 ) ,257-8 More Information

Li-Kroeger, David; Witt, Lorraine M; Grimes, H Leighton; Cook, Tiffany A; Gebelein, Brian (2008. ) Hox and senseless antagonism functions as a molecular switch to regulate EGF secretion in the Drosophila PNS.Developmental cell, , 15 (2 ) ,298-308 More Information

Xie, Baotong; Charlton-Perkins, Mark; McDonald, Elizabeth; Gebelein, Brian; Cook, Tiffany (2007. ) Senseless functions as a molecular switch for color photoreceptor differentiation in Drosophila.Development (Cambridge, England), , 134 (23 ) ,4243-53 More Information

Gebelein, Brian; Mann, Richard S (2007. ) Compartmental modulation of abdominal Hox expression by engrailed and sloppy-paired patterns the fly ectoderm.Developmental biology, , 308 (2 ) ,593-605 More Information

Gebelein, Brian; McKay, Daniel J; Mann, Richard S (2004. ) Direct integration of Hox and segmentation gene inputs during Drosophila development.Nature, , 431 (7009 ) ,653-9 More Information

Gebelein, Brian; Culi, Joaquim; Ryoo, Hyung Don; Zhang, Wen; Mann, Richard S (2002. ) Specificity of Distalless repression and limb primordia development by abdominal Hox proteins. Developmental cell, , 3 (4 ) ,487-98

Bessa, Jose; Gebelein, Brian; Pichaud, Franck; Casares, Fernando; Mann, Richard S (2002. ) Combinatorial control of Drosophila eye development by eyeless, homothorax, and teashirt.Genes & development, , 16 (18 ) ,2415-27 More Information

Gresser, Amy L; Gutzwiller, Lisa M; Gauck, Mackenzie K; Hartenstein, Volker; Cook, Tiffany A; Gebelein, Brian (2015. ) Rhomboid Enhancer Activity Defines a Subset of Drosophila Neural Precursors Required for Proper Feeding, Growth and Viability.PloS one, , 10 (8 ) ,e0134915 More Information

Velu, Chinavenmeni S; Chaubey, Aditya; Phelan, James D; Horman, Shane R; Wunderlich, Mark; Guzman, Monica L; Jegga, Anil G; Zeleznik-Le, Nancy J; Chen, Jianjun; Mulloy, James C; Cancelas, Jose A; Jordan, Craig T; Aronow, Bruce J; Marcucci, Guido; Bhat, Balkrishen; Gebelein, Brian; Grimes, H Leighton (2014. ) Therapeutic antagonists of microRNAs deplete leukemia-initiating cell activity.The Journal of clinical investigation, , 124 (1 ) ,222-36 More Information

Jukam, David; Xie, Baotong; Rister, Jens; Terrell, David; Charlton-Perkins, Mark; Pistillo, Daniela; Gebelein, Brian; Desplan, Claude; Cook, Tiffany (2013. ) Opposite feedbacks in the Hippo pathway for growth control and neural fate.Science (New York, N.Y.), , 342 (6155 ) ,1238016 More Information

Del Águila, Ángela; Adam, Mike; Ullom, Kristy; Shaw, Nicholas; Qin, Shenyue; Ehrman, Jacqueline; Nardini, Diana; Salomone, Joseph; Gebelein, Brian; Lu, Q Richard; Potter, Steven S; Waclaw, Ronald; Campbell, Kenneth; Nakafuku, Masato (2022. ) Olig2 defines a subset of neural stem cells that produce specific olfactory bulb interneuron subtypes in the subventricular zone of adult mice.Development (Cambridge, England), , 149 (5 ) , More Information

Neilson, Derek E; Zech, Michael; Hufnagel, Robert B; Slone, Jesse; Wang, Xinjian; Homan, Shelli; Gutzwiller, Lisa M; Leslie, Elizabeth J; Leslie, Nancy D; Xiao, Jianfeng; Hedera, Peter; LeDoux, Mark S; Gebelein, Brian; Wilbert, Friederike; Eckenweiler, Matthias; Winkelmann, Juliane; Gilbert, Donald L; Huang, Taosheng (2022. ) A Novel Variant of ATP5MC3 Associated with Both Dystonia and Spastic Paraplegia.Movement disorders : official journal of the Movement Disorder Society, , 37 (2 ) ,375-383 More Information

Cain, Brittany; Gebelein, Brian (2021. ) Mechanisms Underlying Hox-Mediated Transcriptional Outcomes.Frontiers in cell and developmental biology, , 9 ,787339 More Information

Kuang, Yi; Pyo, Anna; Eafergan, Natanel; Cain, Brittany; Gutzwiller, Lisa M; Axelrod, Ofri; Gagliani, Ellen K; Weirauch, Matthew T; Kopan, Raphael; Kovall, Rhett A; Sprinzak, David; Gebelein, Brian (2021. ) Enhancers with cooperative Notch binding sites are more resistant to regulation by the Hairless co-repressor.PLoS genetics, , 17 (9 ) ,e1009039 More Information

Oatman, Nicole; Dasgupta, Nupur; Arora, Priyanka; Choi, Kwangmin; Gawali, Mruniya V; Gupta, Nishtha; Parameswaran, Sreeja; Salomone, Joseph; Reisz, Julie A; Lawler, Sean; Furnari, Frank; Brennan, Cameron; Wu, Jianqiang; Sallans, Larry; Gudelsky, Gary; Desai, Pankaj; Gebelein, Brian; Weirauch, Matthew T; D'Alessandro, Angelo; Komurov, Kakajan; Dasgupta, Biplab (2021. ) Mechanisms of stearoyl CoA desaturase inhibitor sensitivity and acquired resistance in cancer.Science advances, , 7 (7 ) , More Information

Salomone, Joseph; Qin, Shenyue; Fufa, Temesgen D; Cain, Brittany; Farrow, Edward; Guan, Bin; Hufnagel, Robert B; Nakafuku, Masato; Lim, Hee-Woong; Campbell, Kenneth; Gebelein, Brian (2021. ) Conserved Gsx2/Ind homeodomain monomer versus homodimer DNA binding defines regulatory outcomes in flies and mice.Genes & development, , 35 (1-2 ) ,157-174 More Information

Kobia, Francis M; Preusse, Kristina; Dai, Quanhui; Weaver, Nicholas; Hass, Matthew R; Chaturvedi, Praneet; Stein, Sarah J; Pear, Warren S; Yuan, Zhenyu; Kovall, Rhett A; Kuang, Yi; Eafergen, Natanel; Sprinzak, David; Gebelein, Brian; Brunskill, Eric W; Kopan, Raphael (2020. ) Notch dimerization and gene dosage are important for normal heart development, intestinal stem cell maintenance, and splenic marginal zone B-cell homeostasis during mite infestation.PLoS biology, , 18 (10 ) ,e3000850 More Information

Elliott, Kelsey H; Chen, Xiaoting; Salomone, Joseph; Chaturvedi, Praneet; Schultz, Preston A; Balchand, Sai K; Servetas, Jeffrey D; Zuniga, Aimée; Zeller, Rolf; Gebelein, Brian; Weirauch, Matthew T; Peterson, Kevin A; Brugmann, Samantha A (2020. ) Gli3 utilizes Hand2 to synergistically regulate tissue-specific transcriptional networks.eLife, , 9 , More Information

Kuang, Yi; Golan, Ohad; Preusse, Kristina; Cain, Brittany; Christensen, Collin J; Salomone, Joseph; Campbell, Ian; Okwubido-Williams, FearGod V; Hass, Matthew R; Yuan, Zhenyu; Eafergan, Nathanel; Moberg, Kenneth H; Kovall, Rhett A; Kopan, Raphael; Sprinzak, David; Gebelein, Brian (2020. ) Enhancer architecture sensitizes cell specific responses to Notch gene dose via a bind and discard mechanism.eLife, , 9 , More Information

Roychoudhury, Kaushik; Salomone, Joseph; Qin, Shenyue; Cain, Brittany; Adam, Mike; Potter, S Steven; Nakafuku, Masato; Gebelein, Brian; Campbell, Kenneth (2020. ) Physical interactions between Gsx2 and Ascl1 balance progenitor expansion versus neurogenesis in the mouse lateral ganglionic eminence.Development (Cambridge, England), , 147 (7 ) , More Information

Zhang, Xinghao; McGrath, Patrick S; Salomone, Joseph; Rahal, Mohamed; McCauley, Heather A; Schweitzer, Jamie; Kovall, Rhett; Gebelein, Brian; Wells, James M (2019. ) A Comprehensive Structure-Function Study of Neurogenin3 Disease-Causing Alleles during Human Pancreas and Intestinal Organoid Development.Developmental cell, , 50 (3 ) ,367-380.e7 More Information

Zandvakili, Arya; Uhl, Juli D; Campbell, Ian; Salomone, Joseph; Song, Yuntao Charlie; Gebelein, Brian (2019. ) The cis-regulatory logic underlying abdominal Hox-mediated repression versus activation of regulatory elements in Drosophila.Developmental biology, , 445 (2 ) ,226-236 More Information

Zandvakili, Arya; Campbell, Ian; Gutzwiller, Lisa M; Weirauch, Matthew T; Gebelein, Brian (2018. ) Degenerate Pax2 and Senseless binding motifs improve detection of low-affinity sites required for enhancer specificity.PLoS genetics, , 14 (4 ) ,e1007289 More Information

Gu, Zirong; Kalambogias, John; Yoshioka, Shin; Han, Wenqi; Li, Zhuo; Kawasawa, Yuka Imamura; Pochareddy, Sirisha; Li, Zhen; Liu, Fuchen; Xu, Xuming; Wijeratne, H. R. Sagara; Ueno, Masaki; Blatz, Emily; Salomone, Joseph; Kumanogoh, Atsushi; Rasin, Mladen-Roko; Gebelein, Brian; Weirauch, Matthew T; Sestan, Nenad; Martin, John H; Yoshida, Yutaka (2017. ) Control of species-dependent cortico-motoneuronal connections underlying manual dexterity.Science (New York, N.Y.), , 357 (6349 ) ,400-404 More Information

Wang, Guolun; Gutzwiller, Lisa; Li-Kroeger, David; Gebelein, Brian (2017. ) A Hox complex activates and potentiates the Epidermal Growth Factor signaling pathway to specify Drosophila oenocytes.PLoS genetics, , 13 (7 ) ,e1006910 More Information

Kovall, Rhett A; Gebelein, Brian; Sprinzak, David; Kopan, Raphael (2017. ) The Canonical Notch Signaling Pathway: Structural and Biochemical Insights into Shape, Sugar, and Force.Developmental cell, , 41 (3 ) ,228-241 More Information

Zandvakili, Arya; Gebelein, Brian (2016. ) Mechanisms of Specificity for Hox Factor Activity.Journal of developmental biology, , 4 (2 ) , More Information

Uhl, Juli D; Zandvakili, Arya; Gebelein, Brian (2016. ) A Hox Transcription Factor Collective Binds a Highly Conserved Distal-less cis-Regulatory Module to Generate Robust Transcriptional Outcomes.PLoS genetics, , 12 (4 ) ,e1005981 More Information

CCHMC S Building
Phone: 513-636-3366
brian.gebelein@cchmc.org