Ken Greis

Ken D. Greis

Associate Dean

Vontz Center

2304

COM Dean Office of Research - 0521

Vontz Center for Molecular Studies
Room 2304
Cincinnati, Ohio

Professional Summary


Ken Greis earned his PhD in Biochemistry from the University of Kentucky working with Prof. Sam Turco to understand the structure/function relationship of glycoconjugate survival factors produced by protozoan parasites.  He subsequently did postdoctoral research to evaluate the role of glycosylation on tegument proteins in human cytomegalovirus infections at Johns Hopkins under the mentorship of Professors Gerald Hart and Wade Gibson.  During a second postdoctoral stint with Prof. Hart at the University of Alabama at Birmingham, Dr. Greis began to transition his research interest into the newly emerging field of protein mass spectrometry in collaboration with Prof. Steve Barnes.   For the past 30 years, his research has focused on technology development and the application of mass spectrometry to understand biochemical and biomedical systems.  From 1996-2006, he built and lead protein characterization, mass spectrometry and proteomics groups at two pharmaceutical companies-Parke-Davis Pharmaceutical Research (now part of Pfizer) and Procter & Gamble Pharmaceuticals.  He has developed significant expertise in mass spectrometry technology development and project management while collaborating in a variety of research areas including: signal transduction, angiogenesis, metabolic regulation (obesity and diabetes), anti-microbial, musculoskeletal, inflammation, cardiovascular and cancer.  In July 2006, Dr. Greis joined the University of Cincinnati faculty where he is now a Professor of Cancer Biology and the Director of Proteomics and Mass Spectrometry for the University and Cincinnati Children's Hospital Medical Center.  He also served as the Director of the Graduate Program in Cancer and Cell Biology from 2012-2017 and then transitioned into a part time administrative role as Associate Dean for Research Core Facilities in the UC College of Medicine in 2017 and as the Executive Director of Research Infrastructure and Facilities in the Office for the UC Vice President for Research in 2019.  His independent research is focused on global PTM profiling by both label-free and isotope tagging quantitative mass spectrometry to understand aberrant signaling mechanisms as new therapeutic targets for disease.
 

Education

Bachelor's Degree, Centre College 1986 (Biology)

Doctoral Degree, University of Kentucky 1991 (Biochemistry)

Post-Doctoral Training, Johns Hopkins School of Medicine 1993 (Biochemistry/Glycobiology)

Post-Doctoral Training, University of Alabama at Birmingham 1996 (Biochemistry/Mass Spectrom.)

Positions and Work Experience

1996 -1997 Scientist, Department of Chemistry, Parke-Davis Pharmaceutical Research, Ann Arbor, MI.

1997 -1998 Senior Scientist, Protein Chemistry & Mass Spectrometry, Parke-Davis Pharmaceutical Research, Ann Arbor, MI.

1998 - Principal Scientist,, Protein Chemistry & Mass Spectrometry, Parke-Davis Pharmaceutical Research, Ann Arbor, MI.

1998 -2000 Scientist, Structural Biology Section, Group leader Biological Mass Spectrometry, Procter & Gamble Pharmaceuticals, Mason, OH.

2000 -2006 Senior Scientist, Core Discovery Technologies Department, Group leader Biological Mass Spectrometry, Procter & Gamble Pharmaceuticals, Mason, OH.

2006 -2008 Associate Professor, Department of Genome Science, University of Cincinnati, Cincinnati, OH.

2006 -To Present Director, Proteomics and Mass Spectrometry, University of Cincinnati and the Cincinnati Children’s Hospital Medical Center., Cincinnati, OH

2008 -2012 Associate Professor, Department of Cancer and Cell Biology, University of Cincinnati, Cincinnati, OH

2012 -2015 Associate Professor,, Department of Cancer Biology, College of Medicine, University of Cincinnati,

2015 -To Present Professor of Cancer Biology, University of Cincinnati,

2013 -To Present Scientific Director,, UC College of Medicine Research Core Facilities,

2013 -2017 Director, Graduate Program in Cancer and Cell Biology, UC College of Medicine,

2017 -To Present Associate Dean for Research Core Facilities, , UC College of Medicine,

2019 -To Present Executive Director for Research Infrastructure and Facilities, Office of the Vice President for Research,, University of Cincinnati

Research and Practice Interests

My research is dedicated toward integrating mass spectrometry-based technology to understand biological systems.  As such my laborotory collaborates with many investigators across the University of Cincinnati, Cincinnati Childrens Hospital Medical Center and outside institutions and businesses.  Currently my two primary research focuses are in the area of quantitative phosphorylation profiling and the use of mass spectrometry for enzyme assays and high throughput inhibitor screening.

Research Support

Grant: #W81XWH-07-2-0026 Investigators:PI: Maria Czyzyk-Krzeska, MD, PhD 03-2007 -02-2010 DoD; Army Identification of Genes in Kidney Cancer Oncogenesis Role:Co-I Completed

Grant: #SRS 005129 Investigators:Greis, Kenneth 10-01-2007 -12-30-2009 MDS Analytical Technologies Exploiting MALDI-MS as a Label-Free Approach to Multiplex Enzyme Inhibitor Screening Role:PI $202,000.00 Completed Type:Grant

Grant: #R01 AI072581 Investigators:Diaz-Meco, Maria; Greis, Kenneth; Moscat, Jorge 01-15-2008 -12-31-2012 National Institute of Allergy and Infectious Diseases The p62/atypical PKC Signaling Complex in Th2 Differentiation and Asthma Role:Co-I $1,166,100.00 Completed

Grant: #P30 ES006096-16A1 Investigators:Caruso, Joseph; Deka, Ranjan; Embi, Peter; Greis, Kenneth; Ho, Shuk Mei; Lemasters, Grace; Medvedovic, Mario; Meller, Jaroslaw; Nebert, Daniel; Pinney, Susan; Puga, Alvaro; Rohs, Amy; Woo, Daniel 04-01-2008 -03-31-2013 National Institute of Environmental Health Sciences Environmental Genetics Role:Collaborator $8,028,782.00 Completed

Grant: #NIEHS P30-ES06096 Investigators:PI: Shuk-mei Ho, PhD 04-01-2008 -03-31-2013 Program Grant support of the Center for Environmental Genetics (CEG). The unifying research focus of the Center is to study the impact of genetic diversity on individual susceptibility to deleterious environmental agents. Role:Co-I Completed Type:Grant

Grant: #NIH R01 DK078019-01A2 Investigators:PI: George Thomas, PhD 07-01-2008 -06-30-2013 Role of hVps34/mTOR Complex 1 in Amino Acid-Induced Obesity and Insulin Resistance Role:Co-I Completed

Grant: #R01 DK078019 Investigators:Greis, Kenneth; Kozma, Sara; Thomas, George 07-15-2008 -05-31-2013 National Inst of Diabetes and Digestive and Kidney Disease Role of hVps34/mTOR Complex in Amino Acid-Induced Obesity and Insulin Resistance Role:Co-I $1,371,652.00 Completed

Grant: #NIH 1U54 1KL2RR026315-0109 Investigators:PI: J. Heubi, MD 04-03-2009 -03-31-2014 Institutional Clinical and Translational Science Award Role:Co-Investigator-Core Leader

Grant: #R01 DK082583 Investigators:Greis, Kenneth; Jaeschke, Anja; Obici, Silvana; Shu, Hongjun 08-01-2010 -07-31-2015 National Inst of Diabetes and Digestive and Kidney Disease MLK3 in Metabolic Stress Signaling Role:Co-I $1,348,763.00 Active

Grant: #NIH R01 DK082583-01-A1 Investigators:PI: A. Jaeschke, PhD 08-01-2010 -07-31-2015 MLK3 in metabolic stress signaling Role:Co-I Type:Grant

Grant: #NIH R01 NIH HL075633-02-A2 Investigators:PI: J.-E. Schultz, PhD 09-01-2010 -08-31-2015 Signaling mechanisms of FGF2-induced cardioprotection Role:Co-I Type:Grant

Grant: # NIH S10 RR027015-01 Investigators:PI: Ken Greis, PhD 09-01-2010 -08-31-2011 Enhanced proteomics capabilities for quantitation of proteins with a triple quadrupole mass spectrometer Role:PI Type:Grant

Grant: #S10 RR027015 Investigators:Greis, Kenneth; Haffey, Wendy; Schultz, Jo El; Shu, Hongjun 09-07-2010 -09-06-2011 National Center for Research Resources Enhanced Proteomics Capabilities for Quantitation of Proteins with a Triple Quadrupole Mass Spectrometer Role:PI $426,215.00 Completed

Grant: #SRS 007642 Investigators:Czyzyk-Krzeska, Maria; Greis, Kenneth 07-01-2011 -06-30-2015 Department of Veterans Affairs Intergovernmental Personnel Act Agreement for Ken Greis Role:Collaborator $45,372.00 Active

Grant: #109825 Investigators:Greis, Kenneth 07-01-2011 -06-30-2013 St. Baldrick's Foundation Mechanisms Underlying DICER1 Suppression of Pleuropulmonary Blastoma Role:PI $102,283.00 Active Level:Private Non-Profit

Grant: #NIH R21 HL109635-01 Investigators:PI: M. Borchers, PhD 08-15-2011 -07-31-2013 NOVEL REAGENTS IN MOUSE MODELS OF AUTOIMMUNE COPD Role:Co-I Completed Type:Grant

Grant: #R21 HL109635 Investigators:Borchers, Michael; Greis, Kenneth 08-15-2011 -07-31-2013 National Heart, Lung and Blood Institute Novel Reagents in Mouse Models of Autoimmune COPD Role:Co-I $431,750.00 Completed

Grant: #R33 CA151210 Investigators:Greis, Kenneth; Nelson, Sandra; Rathore, Rakesh 09-27-2011 -08-31-2014 National Cancer Institute Validation of MALDI-MS-Based Inhibitor Screening Technologies for Cancer Targets Role:PI $469,178.00 Active Level:Federal

Grant: #131862 \ P50DK096418-01 Investigators:Greis, Kenneth 09-21-2012 -08-31-2017 National Inst of Diabetes and Digestive and Kidney Disease Critical Translational Studies in Pediatric Nephrology Role:PI $86,125.00 Active Level:Federal

Grant: #P30ES006096 Investigators:Caruso, Joseph; Chen, Aimin; Deka, Ranjan; Greis, Kenneth; Haynes, Erin; Ho, Shuk Mei; Lemasters, Grace; Leung, Yuet Kin; Medvedovic, Mario; Meller, Jaroslaw; Meloncon, Lisa; Pinney, Susan; Porollo, Alexey; Puga, Alvaro; Thomas, Michael; Woo, Daniel; Yadav, Jagjit; Yancey, Elissa 04-01-2013 -03-31-2018 National Institute of Environmental Health Sciences Environmental Genetics Role:Collaborator $1,743,768.00 Active Level:Federal

Grant: #R01 HL119538 Investigators:Borchers, Michael; Greis, Kenneth 04-01-2014 -03-31-2018 National Heart, Lung and Blood Institute Clec5a regulation of Macrophage function in COPD Role:Collaborator $396,250.00 Awarded Level:Federal

Grant: #137135 / F31HL132468 Investigators:Althoff, Mark; Greis, Kenneth 04-01-2016 -03-31-2019 National Heart, Lung and Blood Institute Scribble in hematopoietic stem cell activity Role:Collaborator $31,836.00 Active Level:Federal

Grant: #R21MH107916 Investigators:Funk, Adam; Greis, Kenneth; McCullumsmith, Robert; Meller, Jaroslaw 05-13-2016 -04-30-2018 National Institute of Mental Health Proteomic analysis of the postsynaptic density-95 interactome in schizophrenia Role:Collaborator $237,000.00 Awarded Level:Federal

Grant: #IOS-1755202 Investigators:Greis, Kenneth 07-01-2018 -06-30-2021 National Science Foundation Collaborative Research: Ammonia oxidizers and their heterotrophic friends Role:PI $137,432.00 Awarded Level:Federal

Grant: #137128 / F31 HL132420 Investigators:Greis, Kenneth; Smith, Molly 09-01-2016 -08-31-2020 National Heart, Lung and Blood Institute Aberrant Ubiquitin-Editing in the Pathogenesis of Myeloid Malignancies Role:Collaborator $102,687.00 Active Level:Federal

Grant: #415810 / DE-AC05-76RL01830 Investigators:Barrett, William; Greis, Kenneth; Lamba, Michael 06-22-2018 -04-30-2020 Department of Energy Ceium Irradiator Replacement Program - University of Cincinnati Proposal Number 463974 Role:PI $203,000.00 Active Level:Federal

Grant: #VA IPA Greis Investigators:Greis, Kenneth 04-01-2018 -03-31-2020 Department of Veterans Affairs IPA for Ken D. Greis Role:PI $8,070.96 Active Level:Federal

Grant: #R01HL141236 Investigators:Borchers, Michael; Greis, Kenneth 01-01-2019 -12-31-2022 National Heart, Lung and Blood Institute Natural Killer Cell Functions in Lymphangioleiomyomatosis Role:Collaborator $388,340.00 Awarded Level:Federal

Grant: #1S10OD026717-01 Investigators:Greis, Kenneth 08-01-2019 -07-31-2020 Office of the Director, National Institutes of Health An Orbitrap Mass Spectrometry System for the University of Cincinnati Proteomics Laboratory Role:PI $815,978.00 Awarded Level:Federal

Grant: #R01GM128216 Investigators:Czyzyk-Krzeska, Maria; Greis, Kenneth; Guan, Jun-lin; Meller, Jaroslaw 09-12-2019 -08-31-2023 National Institute of General Medical Sciences Mechanisms of selective autophagy Role:Collaborator $314,708.00 Awarded Level:Federal

Grant: #CHMC 309380 sub R01EY030251 Investigators:Greis, Kenneth 09-30-2019 -06-30-2021 National Eye Institute Predicting uveitis onset in children with juvenile idiopathic arthritis Role:PI $86,253.00 Active Level:Federal

Publications

Peer Reviewed Publications

Bennett, Michael R; Pleasant, LaTawnya; Haffner, Christopher; Ma, Qing; Haffey, Wendy D; Ying, Jun; Wagner, Michael; Greis, Kenneth D; Devarajan, Prasad (2017. )A Novel Biomarker Panel to Identify Steroid Resistance in Childhood Idiopathic Nephrotic Syndrome.Biomarker insights, ,12 ,1177271917695832

Dwivedi, Pankaj; Greis, Kenneth D (2017. )Granulocyte colony-stimulating factor receptor signaling in severe congenital neutropenia, chronic neutrophilic leukemia, and related malignancies.Experimental hematology, ,46 ,9-20

Fang, Jing; Bolanos, Lyndsey C; Choi, Kwangmin; Liu, Xiaona; Christie, Susanne; Akunuru, Shailaja; Kumar, Rupali; Wang, Dehua; Chen, Xiaoting; Greis, Kenneth D; Stoilov, Peter; Filippi, Marie-Dominique; Maciejewski, Jaroslaw P; Garcia-Manero, Guillermo; Weirauch, Matthew T; Salomonis, Nathan; Geiger, Hartmut; Zheng, Yi; Starczynowski, Daniel T (2017. )Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia.Nature immunology, ,18 (2 ),236-245

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B (2016. )Selective disulfide reduction for labeling and enhancement of Fab antibody fragments.Biochemical and biophysical research communications, ,480 (4 ),752-757

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B (2016. )Structural characterization of expressed monoclonal antibodies by single sample mass spectral analysis after IdeS proteolysis.Biochemical and biophysical research communications, ,477 (3 ),363-8

Sedlacek, Christopher J; Nielsen, Susanne; Greis, Kenneth D; Haffey, Wendy D; Revsbech, Niels Peter; Ticak, Tomislav; Laanbroek, Hendrikus J; Bollmann, Annette (2016. )Effects of Bacterial Community Members on the Proteome of the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain Is79.Applied and environmental microbiology, ,82 (15 ),4776-88

Blanco, Víctor M; Chu, Zhengtao; LaSance, Kathleen; Gray, Brian D; Yan Pak, Koon; Rider, Therese; Greis, Kenneth D; Qi, Xiaoyang (2016. )Optical and nuclear imaging of glioblastoma with phosphatidylserine-targeted nanovesicles.Oncotarget, ,

Heaven, Michael R; Funk, Adam J; Cobbs, Archie L; Haffey, Wendy D; Norris, Jeremy L; McCullumsmith, Robert E; Greis, Kenneth D (2016. )Systematic evaluation of data-independent acquisition for sensitive and reproducible proteomics-a prototype design for a single injection assay.Journal of mass spectrometry : JMS, ,51 (1 ),1-11

Thowfeik, Fathima Shazna; AbdulSalam, Safnas F; Wunderlich, Mark; Wyder, Michael; Greis, Kenneth D; Kadekaro, Ana L; Mulloy, James C; Merino, Edward J (2015. )A ROS-Activatable Agent Elicits Homologous Recombination DNA Repair and Synergizes with Pathway Compounds.Chembiochem : a European journal of chemical biology, ,16 (17 ),2513-21

Marsh, J M; Davis, M G; Flagler, M J; Sun, Y; Chaudhary, T; Mamak, M; McComb, D W; Williams, R E A; Greis, K D; Rubio, L; Coderch, L (2015. )Advanced hair damage model from ultra-violet radiation in the presence of copper.International journal of cosmetic science, ,37 (5 ),532-41

Wijeratne, Aruna B; Wijesundera, Dharshana N; Paulose, Maggie; Ahiabu, Ivy Belinda; Chu, Wei-Kan; Varghese, Oomman K; Greis, Kenneth D (2015. )Phosphopeptide separation using radially aligned titania nanotubes on titanium wire.ACS applied materials & interfaces, ,7 (21 ),11155-64

Lu, Xiaoming; Zoller, Erin E; Weirauch, Matthew T; Wu, Zhiguo; Namjou, Bahram; Williams, Adrienne H; Ziegler, Julie T; Comeau, Mary E; Marion, Miranda C; Glenn, Stuart B; Adler, Adam; Shen, Nan; Nath, Swapan K; Stevens, Anne M; Freedman, Barry I; Tsao, Betty P; Jacob, Chaim O; Kamen, Diane L; Brown, Elizabeth E; Gilkeson, Gary S; Alarcón, Graciela S; Reveille, John D; Anaya, Juan-Manuel; James, Judith A; Sivils, Kathy L; Criswell, Lindsey A; Vilá, Luis M; Alarcón-Riquelme, Marta E; Petri, Michelle; Scofield, R Hal; Kimberly, Robert P; Ramsey-Goldman, Rosalind; Joo, Young Bin; Choi, Jeongim; Bae, Sang-Cheol; Boackle, Susan A; Graham, Deborah Cunninghame; Vyse, Timothy J; Guthridge, Joel M; Gaffney, Patrick M; Langefeld, Carl D; Kelly, Jennifer A; Greis, Kenneth D; Kaufman, Kenneth M; Harley, John B; Kottyan, Leah C (2015. )Lupus Risk Variant Increases pSTAT1 Binding and Decreases ETS1 Expression.American journal of human genetics, ,96 (5 ),731-9

Hasegawa, Kazuteru; Sin, Ho-Su; Maezawa, So; Broering, Tyler J; Kartashov, Andrey V; Alavattam, Kris G; Ichijima, Yosuke; Zhang, Fan; Bacon, W Clark; Greis, Kenneth D; Andreassen, Paul R; Barski, Artem; Namekawa, Satoshi H (2015. )SCML2 establishes the male germline epigenome through regulation of histone H2A ubiquitination.Developmental cell, ,32 (5 ),574-88

Huang, Yan; Powers, Corey; Madala, Satish K; Greis, Kenneth D; Haffey, Wendy D; Towbin, Jeffrey A; Purevjav, Enkhsaikhan; Javadov, Sabzali; Strauss, Arnold W; Khuchua, Zaza (2015. )Cardiac metabolic pathways affected in the mouse model of barth syndrome.PloS one, ,10 (6 ),e0128561

Huang,Y., Powers,C., Madala,S. K., Greis,K. D., Haffey,W. D., Towbin,J. A., Purevjav,E., Javadov,S., Strauss,A. W., Khuchua,Z. (2015. )Cardiac metabolic pathways affected in the mouse model of barth syndrome .PloS one, ,10 (6 ),e0128561

Marsh,J. M., Davis,M. G., Flagler,M. J., Sun,Y., Chaudhary,T., Mamak,M., McComb,D. W., Williams,R. E., Greis,K. D., Rubio,L., Coderch,L. (2015. )Advanced hair damage model from ultra-violet radiation in the presence of copper .International Journal of Cosmetic Science, ,37 (5 ),532-41

Wijeratne,A. B., Wijesundera,D. N., Paulose,M., Ahiabu,I. B., Chu,W. K., Varghese,O. K., Greis,K. D. (2015. )Phosphopeptide separation using radially aligned titania nanotubes on titanium wire .ACS applied materials & interfaces, ,7 (21 ),11155 -11164

Marsh, JM; Iveson, R; Flagler, MJ; Davis, MG; Newland, AB; Greis, KD; Sun, Y; Chaudhary, T; Aistrup, ER (2014. )Role of copper in photochemical damage to hair.International journal of cosmetic science, ,36 (1 ),32-8

Liou, Benjamin; Haffey, Wendy D; Greis, Kenneth D; Grabowski, Gregory A (2014. )The LIMP-2/SCARB2 binding motif on acid ?-glucosidase: basic and applied implications for Gaucher disease and associated neurodegenerative diseases.The Journal of biological chemistry, ,289 (43 ),30063-74

Liou B.;Haffey W.;Greis K.;Grabowski G. (2014. )The LIMP-2/SCARB2 binding motif on acid Beta-Glucosidase: Basic and applied implications for Gaucher disease and associated neurodegenerative diseases.Journal of Biological Chemistry, ,289 (43 ),30063-74

Green, Julianne V; Orsborn, Kris I; Zhang, Minlu; Tan, Queenie K G; Greis, Kenneth D; Porollo, Alexey; Andes, David R; Long Lu, Jason; Hostetter, Margaret K (2013. )Heparin-binding motifs and biofilm formation by Candida albicans.The Journal of infectious diseases, ,208 (10 ),1695-704

Wijeratne, Aruna B; Manning, Janet R; Schultz, Jo El J; Greis, Kenneth D (2013. )Quantitative phosphoproteomics using acetone-based Peptide labeling: method evaluation and application to a cardiac ischemia/reperfusion model.Journal of proteome research, ,12 (10 ),4268-79

Kattamuri, Chandramohan; Luedeke, David M; Nolan, Kristof; Rankin, Scott A; Greis, Kenneth D; Zorn, Aaron M; Thompson, Thomas B (2012. )Members of the DAN family are BMP antagonists that form highly stable noncovalent dimers.Journal of molecular biology, ,424 (5 ),313-27

Gauthamadasa, Kekulawalage; Vaitinadin, Nataraja Sarma; Dressman, James L; Macha, Stephen; Homan, Reyn; Greis, Kenneth D; Silva, R A Gangani D (2012. )Apolipoprotein A-II-mediated conformational changes of apolipoprotein A-I in discoidal high density lipoproteins.The Journal of biological chemistry, ,287 (10 ),7615-25

Govindan, Suresh; McElligott, Andrew; Muthusamy, Saminathan; Nair, Nandini; Barefield, David; Martin, Jody L; Gongora, Enrique; Greis, Kenneth D; Luther, Pradeep K; Winegrad, Saul; Henderson, Kyle K; Sadayappan, Sakthivel (2012. )Cardiac myosin binding protein-C is a potential diagnostic biomarker for myocardial infarction.Journal of molecular and cellular cardiology, ,52 (1 ),154-64

Piyaphanee, Nuntawan; Ma, Qing; Kremen, Oran; Czech, Kimberly; Greis, Kenneth; Mitsnefes, Mark; Devarajan, Prasad; Bennett, Michael R (2011. )Discovery and initial validation of ? 1-B glycoprotein fragmentation as a differential urinary biomarker in pediatric steroid-resistant nephrotic syndrome.Proteomics. Clinical applications, ,5 (5-6 ),334-42

Linares, Juan F; Amanchy, Ramars; Greis, Kenneth; Diaz-Meco, Maria T; Moscat, Jorge (2011. )Phosphorylation of p62 by cdk1 controls the timely transit of cells through mitosis and tumor cell proliferation.Molecular and cellular biology, ,31 (1 ),105-17

Myer, David L; Robbins, Susan B; Yin, Moying; Boivin, Gregory P; Liu, Yang; Greis, Kenneth D; Bahassi, El Mustapha; Stambrook, Peter J (2011. )Absence of polo-like kinase 3 in mice stabilizes Cdc25A after DNA damage but is not sufficient to produce tumors.Mutation research, ,714 (1-2 ),1-10

Djung, Jane F; Mears, Richard J; Montalbetti, Christian A G N; Coulter, Thomas S; Golebiowski, Adam; Carr, Andrew N; Barker, Oliver; Greis, Kenneth D; Zhou, Songtao; Dolan, Elizabeth; Davis, Gregory F (2011. )The synthesis and evaluation of indolylureas as PKC? inhibitors.Bioorganic & medicinal chemistry, ,19 (8 ),2742-50

Ridsdale, Ross; Na, Cheng-Lun; Xu, Yan; Greis, Kenneth D; Weaver, Timothy (2011. )Comparative proteomic analysis of lung lamellar bodies and lysosome-related organelles.PloS one, ,6 (1 ),e16482

Linares J.;Amanchy R.;Greis K.;Diaz-Meco M.;Moscat J. (2011. )Author's correction: Phosphorylation of p62 by cdk1 controls the timely transit of cells through mit.Molecular and Cellular Biology, ,31 (10 ),2171

Rathore, Rakesh; Pribil, Patrick; Corr, Jay J; Seibel, William L; Evdokimov, Artem; Greis, Kenneth D (2010. )Multiplex Enzyme Assays and Inhibitor Screening by Mass Spectrometry.Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening, ,

Rathore, Rakesh; Corr, Jay J; Lebre, Daniel T; Seibel, William L; Greis, Kenneth D (2009. )Extending matrix-assisted laser desorption/ionization triple quadrupole mass spectrometry enzyme screening assays to targets with small molecule substrates.Rapid communications in mass spectrometry : RCM, ,23 (20 ),3293-300

Aslan, Joseph E; You, Huihong; Williamson, Danielle M; Endig, Jessica; Youker, Robert T; Thomas, Laurel; Shu, Hongjun; Du, Yuhong; Milewski, Robert L; Brush, Matthew H; Possemato, Anthony; Sprott, Kam; Fu, Haian; Greis, Kenneth D; Runckel, Douglas N; Vogel, Arndt; Thomas, Gary (2009. )Akt and 14-3-3 control a PACS-2 homeostatic switch that integrates membrane traffic with TRAIL-induced apoptosis.Molecular cell, ,34 (4 ),497-509

Suzuki, Michiko; Wiers, Kristina; Brooks, Elizabeth B; Greis, Kenneth D; Haines, Kathleen; Klein-Gitelman, Marisa S; Olson, Judyann; Onel, Karen; O (2009. )Initial validation of a novel protein biomarker panel for active pediatric lupus nephritis.Pediatric research, ,65 (5 ),530-6

Eismann, Thorsten; Huber, Nadine; Shin, Thomas; Kuboki, Satoshi; Galloway, Elizabeth; Wyder, Michael; Edwards, Michael J; Greis, Kenneth D; Shertzer, Howard G; Fisher, Aron B; Lentsch, Alex B (2009. )Peroxiredoxin-6 protects against mitochondrial dysfunction and liver injury during ischemia-reperfusion in mice.American journal of physiology. Gastrointestinal and liver physiology, ,296 (2 ),G266-74

Haffey, Wendy D; Mikhaylova, Olga; Meller, Jarek; Yi, Ying; Greis, Kenneth D; Czyzyk-Krzeska, Maria F (2009. )iTRAQ proteomic identification of pVHL-dependent and -independent targets of Egln1 prolyl hydroxylase knockdown in renal carcinoma cells.Advances in enzyme regulation, ,49 (1 ),121-32

Stella, Caroline L; Bennett, Michael R; Devarajan, Prasad; Greis, Kenneth; Wyder, Michael; Macha, Stephen; Rao, Marepalli; Jodicke, Cristiano; Moussa, Hind; How, Helen Y; Myatt, Leslie; Webster, Rose; Sibai, Baha M (2009. )Preterm labor biomarker discovery in serum using 3 proteomic profiling methodologies.American journal of obstetrics and gynecology, ,201 (4 ),387.e1-13

Rathore, Rakesh; Corr, Jay; Scott, George; Vollmerhaus, Pauline; Greis, Kenneth D (2008. )Development of an inhibitor screening platform via mass spectrometry.Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening, ,13 (10 ),1007-13

Mikhaylova, Olga; Ignacak, Monika L; Barankiewicz, Teresa J; Harbaugh, Svetlana V; Yi, Ying; Maxwell, Patrick H; Schneider, Martin; Van Geyte, Katie; Carmeliet, Peter; Revelo, Monica P; Wyder, Michael; Greis, Kenneth D; Meller, Jarek; Czyzyk-Krzeska, Maria F (2008. )The von Hippel-Lindau tumor suppressor protein and Egl-9-Type proline hydroxylases regulate the large subunit of RNA polymerase II in response to oxidative stress.Molecular and cellular biology, ,28 (8 ),2701-17

Greis, Kenneth D (2007. )Mass spectrometry for enzyme assays and inhibitor screening: an emerging application in pharmaceutical research.Mass spectrometry reviews, ,26 (3 ),324-39

Greis, Kenneth D; Zhou, Songtao; Burt, Thomas M; Carr, Andrew N; Dolan, Elizabeth; Easwaran, Vijayasurian; Evdokimov, Artem; Kawamoto, Richard; Roesgen, Jeff; Davis, Gregory F (2006. )MALDI-TOF MS as a label-free approach to rapid inhibitor screening.Journal of the American Society for Mass Spectrometry, ,17 (6 ),815-22

Greis, Kenneth D; Zhou, Songtao; Siehnel, Richard; Klanke, Chuck; Curnow, Alan; Howard, Jeremy; Layh-Schmitt, Gerlinde (2005. )Development and validation of a whole-cell inhibition assay for bacterial methionine aminopeptidase by surface-enhanced laser desorption ionization-time of flight mass spectrometry.Antimicrobial agents and chemotherapy, ,49 (8 ),3428-34

Jarrold, Bradley; DeMuth, Jeffrey; Greis, Kenneth; Burt, Thomas; Wang, Feng (2005. )An effective skeletal muscle prefractionation method to remove abundant structural proteins for optimized two-dimensional gel electrophoresis .Electrophoresis, ,26 (11 ),2269-78

Thompson, Larry J; Wang, Feng; Proia, Alan D; Peters, Kevin G; Jarrold, Brad; Greis, Kenneth D (2003. )Proteome analysis of the rat cornea during angiogenesis.Proteomics, ,3 (11 ),2258-66

Peters, Kevin G; Davis, Mike G; Howard, Brian W; Pokross, Matthew; Rastogi, Vinit; Diven, Conrad; Greis, Kenneth D; Eby-Wilkens, Elaine; Maier, Matthew; Evdokimov, Artem; Soper, Shari; Genbauffe, Frank (2003. )Mechanism of insulin sensitization by BMOV (bis maltolato oxo vanadium); unliganded vanadium (VO4) as the active component. Journal of inorganic biochemistry, ,96 (2-3 ),321-30

Delinsky, David C; Greis, Kenneth D (2002. )Capillary chromatography-coupled mass spectrometry with column switching for rapid identification of proteins from 2-dimensional electrophoresis gels. Journal of proteome research, ,1 (3 ),279-84

Isfort, Robert J; Wang, Feng; Greis, Kenneth D; Sun, Yiping; Keough, Thomas W; Farrar, Roger P; Bodine, Sue C; Anderson, N Leigh (2002. )Proteomic analysis of rat soleus muscle undergoing hindlimb suspension-induced atrophy and reweighting hypertrophy.Proteomics, ,2 (5 ),543-50

Isfort, Robert J; Wang, Feng; Greis, Kenneth D; Sun, Yiping; Keough, Thomas W; Bodine, Sue C; Anderson, N Leigh (2002. )Proteomic analysis of rat soleus and tibialis anterior muscle following immobilization. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, ,769 (2 ),323-32

Egnaczyk, G F; Greis, K D; Stimson, E R; Maggio, J E (2001. )Photoaffinity cross-linking of Alzheimer's disease amyloid fibrils reveals interstrand contact regions between assembled beta-amyloid peptide subunits. Biochemistry, ,40 (39 ),11706-14

Hoke S.;Morand K.;Greis K.;Baker T.;Harbol K.;Dobson R. (2001. )Transformations in pharmaceutical research and development, driven by innovations in multidimensiona.International Journal of Mass Spectrometry, ,212 (1-3 ),135-196

Smaill, ; Rewcastle, ; Loo, ; Greis, ; Chan, ; Reyner, ; Lipka, ; Showalter, ; Vincent, ; Elliott, ; Denny, (2000. )Tyrosine kinase inhibitors. 17. Irreversible inhibitors of the epidermal growth factor receptor: 4-(Phenylamino)quinazoline- and 4-(Phenylamino)pyrido .Journal of medicinal chemistry, ,43 (16 ),3199

Isfort, R J; Hinkle, R T; Jones, M B; Wang, F; Greis, K D; Sun, Y; Keough, T W; Anderson, N L; Sheldon, R J (2000. )Proteomic analysis of the atrophying rat soleus muscle following denervation.Electrophoresis, ,21 (11 ),2228-34

Smaill, J B; Rewcastle, G W; Loo, J A; Greis, K D; Chan, O H; Reyner, E L; Lipka, E; Showalter, H D; Vincent, P W; Elliott, W L; Denny, W A (2000. )Tyrosine kinase inhibitors. 17. Irreversible inhibitors of the epidermal growth factor receptor: 4-(phenylamino)quinazoline- and 4-(phenylamino)pyrido[3,2-d]pyrimidine-6-acrylamides bearing additional solubilizing functions. Journal of medicinal chemistry, ,43 (7 ),1380-97

Sanchez, V; Greis, K D; Sztul, E; Britt, W J (2000. )Accumulation of virion tegument and envelope proteins in a stable cytoplasmic compartment during human cytomegalovirus replication: characterization of a potential site of virus assembly. Journal of virology, ,74 (2 ),975-86

VanBogelen, R A; Greis, K D; Blumenthal, R M; Tani, T H; Matthews, R G (1999. )Mapping regulatory networks in microbial cells. Trends in microbiology, ,7 (8 ),320-8

Fry, D W; Bridges, A J; Denny, W A; Doherty, A; Greis, K D; Hicks, J L; Hook, K E; Keller, P R; Leopold, W R; Loo, J A; McNamara, D J; Nelson, J M; Sherwood, V; Smaill, J B; Trumpp-Kallmeyer, S; Dobrusin, E M (1998. )Specific, irreversible inactivation of the epidermal growth factor receptor and erbB2, by a new class of tyrosine kinase inhibitor. Proceedings of the National Academy of Sciences of the United States of America, ,95 (20 ),12022-7

Stevenson, T I; Loo, J A; Greis, K D (1998. )Coupling capillary high-performance liquid chromatography to matrix-assisted laser desorption/ionization mass spectrometry and N-terminal sequencing of peptides via automated microblotting onto membrane substrates.Analytical biochemistry, ,262 (2 ),99-109

Greis, K D; Hart, G W (1998. )Analytical methods for the study of O-GlcNAc glycoproteins and glycopeptides.Methods in molecular biology (Clifton, N.J.), ,76 ,19-33

Greis K.D.;Hart G.W. (1997. )Chapter 2 Nuclear and Cytoplasmic Glycoproteins.New Comprehensive Biochemistry, ,29 (PART B ),33-54

Greis, K D; Zhu, S; Matalon, S (1996. )Identification of nitration sites on surfactant protein A by tandem electrospray mass spectrometry.Archives of biochemistry and biophysics, ,335 (2 ),396-402

Greis, K D; Hayes, B K; Comer, F I; Kirk, M; Barnes, S; Lowary, T L; Hart, G W (1996. )Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by beta-elimination and tandem electrospray mass spectrometry.Analytical biochemistry, ,234 (1 ),38-49

Hayes, B K; Greis, K D; Hart, G W (1995. )Specific isolation of O-linked N-acetylglucosamine glycopeptides from complex mixtures.Analytical biochemistry, ,228 (1 ),115-22

Hart, G W; Greis, K D; Dong, L Y; Blomberg, M A; Chou, T Y; Jiang, M S; Roquemore, E P; Snow, D M; Kreppel, L K; Cole, R N (1995. )O-linked N-acetylglucosamine: the "yin-yang" of Ser/Thr phosphorylation? Nuclear and cytoplasmic glycosylation. Advances in experimental medicine and biology, ,376 ,115-23

Hart G.W.;Greis K.D.;Dong L.Y.D.;Blomberg M.A.;Chou T.Y.;Jiang M.S.;Roquemore E.P.;Snow D.M.;Kreppel L.K.;Cole R.C.;Hayes B.K. (1995. )Ubiquitous and temporal glycosylation of nuclear and cytoplasmic proteins.Pure and Applied Chemistry, ,67 (10 ),1637-1645

Greis, K D; Gibson, W; Hart, G W (1994. )Site-specific glycosylation of the human cytomegalovirus tegument basic phosphoprotein (UL32) at serine 921 and serine 952. Journal of virology, ,68 (12 ),8339-49

Thomas, J R; McConville, M J; Thomas-Oates, J E; Homans, S W; Ferguson, M A; Gorin, P A; Greis, K D; Turco, S J (1992. )Refined structure of the lipophosphoglycan of Leishmania donovani. The Journal of biological chemistry, ,267 (10 ),6829-33

Greis, K D; Turco, S J; Thomas, J R; McConville, M J; Homans, S W; Ferguson, M A (1992. )Purification and characterization of an extracellular phosphoglycan from Leishmania donovani. The Journal of biological chemistry, ,267 (9 ),5876-81

Crawford, I T; Greis, K D; Parks, L; Streips, U N (1987. )Facile autoplast generation and transformation in Bacillus thuringiensis subsp. kurstaki. Journal of bacteriology, ,169 (12 ),5423-8

Smith, Eric A; Krumpelbeck, Eric F; Jegga, Anil G; Prell, Malte; Matrka, Marie M; Kappes, Ferdinand; Greis, Kenneth D; Ali, Abdullah M; Meetei, Amom R; Wells, Susanne I (2018. )The nuclear DEK interactome supports multi-functionality.Proteins, ,86 (1 ),88-97

Fang, Jing; Bolanos, Lyndsey C; Choi, Kwangmin; Liu, Xiaona; Christie, Susanne; Akunuru, Shailaja; Kumar, Rupali; Wang, Dehua; Chen, Xiaoting; Greis, Kenneth D; Stoilov, Peter; Filippi, Marie-Dominique; Maciejewski, Jaroslaw P; Garcia-Manero, Guillermo; Weirauch, Matthew T; Salamonis, Nathan; Geiger, Hartmut; Zheng, Yi; Starczynowski, Daniel T (2017. )Corrigendum: Ubiquitination of hnRNPA1 by TRAF6 links chronic innate immune signaling with myelodysplasia.Nature immunology, ,18 (4 ),474

Chutipongtanate, Somchai; Greis, Kenneth D (2018. )Multiplex Biomarker Screening Assay for Urinary Extracellular Vesicles Study: A Targeted Label-Free Proteomic Approach.Scientific reports, ,8 (1 ),15039

Turnier, Jessica L; Brunner, Hermine I; Bennett, Michael; Aleed, Ashwaq; Gulati, Gaurav; Haffey, Wendy D; Thornton, Sherry; Wagner, Michael; Devarajan, Prasad; Witte, David; Greis, Kenneth D; Aronow, Bruce (2018. )Discovery of SERPINA3 as a candidate urinary biomarker of lupus nephritis activity.Rheumatology (Oxford, England), ,

Dwivedi, Pankaj; Muench, David E; Wagner, Michael; Azam, Mohammad; Grimes, H Leighton; Greis, Kenneth D (2018. )Time resolved quantitative phospho-tyrosine analysis reveals Bruton's Tyrosine kinase mediated signaling downstream of the mutated granulocyte-colony stimulating factor receptors.Leukemia, ,

Yuan, Long; Mishra, Rosalin; Patel, Hima; Abdulsalam, Safnas; Greis, Kenneth D; Kadekaro, Ana Luisa; Merino, Edward J; Garrett, Joan T (2018. )Utilization of Reactive Oxygen Species Targeted Therapy to Prolong the Efficacy of BRAF Inhibitors in Melanoma.Journal of Cancer, ,9 (24 ),4665-4676

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B (2018. )Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE.Biochemistry and biophysics reports, ,16 ,138-144

Huang, Huilin; Weng, Hengyou; Sun, Wenju; Qin, Xi; Shi, Hailing; Wu, Huizhe; Zhao, Boxuan Simen; Mesquita, Ana; Liu, Chang; Yuan, Celvie L; Hu, Yueh-Chiang; Hüttelmaier, Stefan; Skibbe, Jennifer R; Su, Rui; Deng, Xiaolan; Dong, Lei; Sun, Miao; Li, Chenying; Nachtergaele, Sigrid; Wang, Yungui; Hu, Chao; Ferchen, Kyle; Greis, Kenneth D; Jiang, Xi; Wei, Minjie; Qu, Lianghu; Guan, Jun-Lin; He, Chuan; Yang, Jianhua; Chen, Jianjun (2018. )Author Correction: Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation.Nature cell biology, ,20 (9 ),1098

Huang, Huilin; Weng, Hengyou; Sun, Wenju; Qin, Xi; Shi, Hailing; Wu, Huizhe; Zhao, Boxuan Simen; Mesquita, Ana; Liu, Chang; Yuan, Celvie L; Hu, Yueh-Chiang; Hüttelmaier, Stefan; Skibbe, Jennifer R; Su, Rui; Deng, Xiaolan; Dong, Lei; Sun, Miao; Li, Chenying; Nachtergaele, Sigrid; Wang, Yungui; Hu, Chao; Ferchen, Kyle; Greis, Kenneth D; Jiang, Xi; Wei, Minjie; Qu, Lianghu; Guan, Jun-Lin; He, Chuan; Yang, Jianhua; Chen, Jianjun (2018. )Recognition of RNA N6-methyladenosine by IGF2BP proteins enhances mRNA stability and translation.Nature cell biology, ,20 (3 ),285-295

Forster, Catherine S; Haffey, Wendy D; Bennett, Michael; Greis, Kenneth D; Devarajan, Prasad (2019. )Identification of Urinary CD44 and Prosaposin as Specific Biomarkers of Urinary Tract Infections in Children With Neurogenic Bladders.Biomarker insights, ,14 ,1177271919835570

Dwivedi, Pankaj; Muench, David E; Wagner, Michael; Azam, Mohammad; Grimes, H Leighton; Greis, Kenneth D (2019. )Phospho serine and threonine analysis of normal and mutated granulocyte colony stimulating factor receptors.Scientific data, ,6 (1 ),21

Turnier JL, Brunner HI, Bennett M, Aleed A, Gulati G, Haffey WD, Thornton S, Wagner M, Devarajan P, Witte D, Greis KD, Aronow B. (2019. )Discovery of SERPINA3 as a candidate urinary biomarker of lupus nephritis activity.Rheumatology, ,58(2) ,321-330

Niederkorn, Madeline; Hueneman, Kathleen; Choi, Kwangmin; Varney, Melinda E; Romano, Laurel; Pujato, Mario A; Greis, Kenneth D; Inoue, Jun-Ichiro; Meetei, Ruhikanta; Starczynowski, Daniel T (2020. )TIFAB Regulates USP15-Mediated p53 Signaling during Stressed and Malignant Hematopoiesis.Cell reports, ,30 (8 ),2776-2790.e6

He, Quan; Wang, Fangfei; Honda, Takashi; Greis, Kenneth D; Redington, Andrew N (2020. )Ablation of miR-144 increases vimentin expression and atherosclerotic plaque formation.Scientific reports, ,10 (1 ),6127

Dwivedi, Pankaj; Chutipongtanate, Somchai; Muench, David E; Azam, Mohammad; Grimes, H Leighton; Greis, Kenneth D (2020. )SWATH-Proteomics of Ibrutinib's Action in Myeloid Leukemia Initiating Mutated G-CSFR Signaling.Proteomics. Clinical applications, ,e1900144

Muench, David E; Olsson, Andre; Ferchen, Kyle; Pham, Giang; Serafin, Rachel A; Chutipongtanate, Somchai; Dwivedi, Pankaj; Song, Baobao; Hay, Stuart; Chetal, Kashish; Trump-Durbin, Lisa R; Mookerjee-Basu, Jayati; Zhang, Kejian; Yu, Jennifer C; Lutzko, Carolyn; Myers, Kasiani C; Nazor, Kristopher L; Greis, Kenneth D; Kappes, Dietmar J; Way, Sing Sing; Salomonis, Nathan; Grimes, H Leighton (2020. )Mouse models of neutropenia reveal progenitor-stage-specific defects.Nature, ,582 (7810 ),109-114

Honors and Awards

1991 -1993 Johns Hopkins/Monsanto Glycosciences Postdoctoral Felllowship

2016 -2017 University of Cincinnati, College of Medicine Research Service Award

2017 -2018 UC College of Medicine Team Science Award

Keywords

Biochemistry, Mass Spectrometry, Proteomics, High Throughput Screening, Protein Identification, Phosphorylation, Post Translational Modifications, Peptide Sequencing, Graduate Education, Career Development, Core Laboratories, Research Management

Professional Affiliation

1994 -To Present: American Society for Mass Spectrometry (ASMS).

1996 -To Present: Association of Biomolecular Resource Facilities (ABRF).

1996 -To Present: American Chemical Society (ACS).

2003: Co-chair & co-organizer of a session entitled: “Emerging Technologies in Proteomics Research” at the 9th Annual Society for Biomolecular Screening conference in Portland, OR. September 22-26.

2005 -2006: Program Project Reviewer—U.S. Department of Energy, Office of Science, Advisor Committee for Genomics-GTL program, SRA: Arthur Katz, Ph.D.

2007: Invited Panelist—DoD, VA, NIH joint Workshop on Occupational and Environmental Risk Factors for ALS. Washington, D.C. June 18, 2007.

2008 -To Present: NIH Study Section: “Applications of Emerging Technologies for Cancer Research.” SRA: Marvin L. Salin, Ph.D.

Patents and Inventions

US7,811,595 Prolyl Hydroxylase Inhibitors and Methods of Use Kawamoto RM, Wu S, Warshokoon NC, Evdokimov AG, Greis KD and Boyer AS, Awarded 10-12-2010 Co-Inventor

US8,940,773 Antibody binding microbial heparin binding motif to retard of prevent microbial biofilm formation on implanted medical devices. Hostetter MK, Lu L, Green JV, Porollo A, Orsborn KI, Tan KGQ, Greis KD, Andes D. , Awarded 01-27-2016 Co-Inventor

US10,478,800B2 HIGHLY ORDERED TITANIA NANOTUBE ARRAYS FOR PHOSPHOPROTEOMICS. Varghese OK, Wijeratne A, Paulose M, Ahiabu I, Greis KD, Wijesundara D, Chu W-K, Awarded 11-19-2019 Co-Inventor

Contact Information

Academic - Vontz Center for Molecular Studies
Room 2304
Cincinnati  Ohio, Phone: 513-558-7102
ken.greis@uc.edu