Andrew Herr

Andrew B. Herr

Assistant Professor of Clinical-Affiliate

Childrens Hospital Bldg R

S5.246

COM Pediatrics Hospital Medicine - 0054

Cardiovascular Research Center
Room 5926
Cincinnati, Ohio 45267

Professional Summary

The Herr laboratory studies protein-protein interactions involved in bacterial pathogenesis, immune regulation, and thrombosis.  We use the complementary techniques of biophysical chemistry and X-ray crystallography to understand how cell-surface proteins recognize their ligands and mediate their biological function.  We have three major areas of investigation:  bacterial surface proteins that mediate intercellular adhesion in biofilms; immune system proteins, including IgA1-receptor interactions implicated in the disease IgA nephropathy; and receptor-collagen interactions leading to platelet activation.  In addition to our major projects, my group works with many other laboratories at our institution and across the country using analytical ultracentrifugation, surface plasmon resonance, circular dichroism, binding analyses, and molecular modeling.

Education

Doctoral Degree, Washington University 1999 (Molecular Biophysics)

Bachelor's Degree, Oral Roberts University 1993 (Biomedical Chemistry)

Positions and Work Experience

2003 -2009 Assistant Professor & Ohio Eminent Scholar, University of Cincinnati College of Medicine, Cincinnati, OH

2009 -2014 Associate Professor & Ohio Eminent Scholar, University of Cincinnati College of Medicine, Cincinnati, OH

2012 -2014 Associate Director, Medical Scientist (MD/PhD) Training Program, University of Cincinnati College of Medicine, Cincinnati, OH

2014 -To Present Associate Professor, Cincinnati Children's Hospital Medical Center, Divisions of Immunobiology & Infectious Diseases and Center for Systems Immunology

Research and Practice Interests

My lab studies protein-protein interactions involved in receptor signaling and bacterial pathogenesis. We use x-ray crystallography to solve the three-dimensional atomic structures of interesting proteins, combined with techniques of biophysical chemistry to understand their interactions in solution. In particular, we use analytical ultracentrifugation, calorimetry, and surface plasmon resonance to study binding and complex formation.

Research Support

Grant: #R01 GM094363 Investigators:Andrew B. Herr 09-01-2011 -06-30-2015 NIH/NIGMS Studies of metal-dependent intercellular adhesion in Staphylococcal biofilms Role:PI Active Type:Grant

Grant: #R01 GM098539 Investigators:Matt B. Renfrow; Andrew B. Herr 09-15-2011 -07-14-2015 NIH/NIGMS Analytical tools for the analysis of clustered O-glycans in clinical samples Role:PI of Subcontract Active Type:Grant

Investigators:Andrew B. Herr, Ph.D. 10-01-2003 -BAD DATE State of Ohio Ohio Eminent Scholar Program Role:PI Active

Grant: #1 R01 DK071802-01A1 Investigators:Herr, Andrew 09-01-2006 -05-31-2012 National Inst of Diabetes and Digestive and Kidney Disease lgA1 Glycosylation and Receptor Interactions in lgA Nephropathy Role:PI $1,370,889.00 Closed Level:Federal

Grant: #AHA 0630128N Investigators:Herr, Andrew 01-01-2006 -12-31-2010 American Heart Association - National Chapter Structural and Functional Studies of Glycoprotein VI (GPVI): Collagen Interactions Role:PI $260,000.00 Closed Level:Private Non-Profit

Grant: #SRS 001868 Investigators:Herr, Andrew 11-01-2005 -10-31-2010 The V Foundation Structure-based Design of an Anti-tumor Antibody to Activate Multiple Immune Pathways Role:PI $100,000.00 Closed Level:Private Non-Profit

Investigators:Herr, Andrew 07-01-2004 -06-30-2005 Leukemia Research Foundation Laying the Groundwork for Design of FcaRI-activating IgG Anti-tumor Antibodies Role:PI $74,348.00 Closed Level:Private Non-Profit

Investigators:Herr, Andrew 05-01-2004 -04-30-2005 Ohio Board of Regents OBR/Ohio Eminent Scholar Structural Biology Role:PI $500,000.00 Closed Level:State of Ohio

Grant: #3 R01 DK071802-04S1 Investigators:Herr, Andrew 09-01-2009 -08-31-2012 National Inst of Diabetes and Digestive and Kidney Disease IgA1 Glycosylation and Receptor Interactions in IgA Nephropathy Role:PI $141,300.00 Closed Level:Federal

Grant: #1 S10 RR027755-01 Investigators:Herr, Andrew; Rance, Mark; Rosevear, Paul 12-10-2009 -12-09-2010 National Center for Research Resources 800 MHz NMR Console Replacement Role:Collaborator $358,323.00 Closed Level:Federal

Grant: #ORISE/Herr Investigators:Herr, Andrew 05-25-2011 -05-24-2012 Department of Energy ORISE Role:PI $62,332.00 Closed Level:Federal

Grant: #13PRE16380011 Investigators:Herr, Andrew; Shelton, Catie 07-01-2013 -06-30-2015 American Heart Association - Great Rivers Affiliate Clues Into Preventing Persistent Cardiovascular Infections by Targeting the Biofilm Protein Aap from S. epidermidis Role:Collaborator $52,000.00 Active Level:Private Non-Profit

Grant: #T32GM063483 Investigators:Deepe, George; Herr, Andrew; Khurana-Hershey, Gurjit 07-01-2013 -06-30-2018 National Institute of General Medical Sciences Medical Scientist Training Program Role:Collaborator $277,195.00 Active Level:Federal

Grant: #1 R01 GM094363-01A1 Investigators:Hassett, Daniel; Herr, Andrew 09-01-2011 -06-30-2015 National Institute of General Medical Sciences Studies of Metal-Dependent Intercellular Adhesion in Staphylococcal Biofilms Role:PI $1,160,325.00 Active Level:Federal

Grant: #UAB# 000398606-001 R01 GM098539 Investigators:Herr, Andrew 09-15-2011 -08-31-2016 National Institute of General Medical Sciences Analytical Tools for the Analysis of Clustered O-glycans in Clinical Samples Role:PI $211,771.00 Active Level:Federal

Publications

Peer Reviewed Publications

Gallegos, Karen M; Conrady, Deborah G; Karve, Sayali S; Gunasekera, Thusitha S; Herr, Andrew B; Weiss, Alison A (2012. ) Shiga toxin binding to glycolipids and glycans.PloS one, , 7 (2 ) ,e30368

Suzuki, Hitoshi; Kiryluk, Krzysztof; Novak, Jan; Moldoveanu, Zina; Herr, Andrew B; Renfrow, Matthew B; Wyatt, Robert J; Scolari, Francesco; Mestecky, Jiri; Gharavi, Ali G; Julian, Bruce A (2011. ) The pathophysiology of IgA nephropathy.Journal of the American Society of Nephrology : JASN, , 22 (10 ) ,1795-803

Hart, Benjamin R; Mishra, Pankaj K; Lintner, Robert E; Hinerman, Jennifer M; Herr, Andrew B; Blumenthal, Robert M (2011. ) Recognition of DNA by the helix-turn-helix global regulatory protein Lrp is modulated by the amino terminus.Journal of bacteriology, , 193 (15 ) ,3794-803

Mani, Timmy; Hennigan, Robert F; Foster, Lauren A; Conrady, Deborah G; Herr, Andrew B; Ip, Wallace (2011. ) FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function.Molecular and cellular biology, , 31 (10 ) ,1983-96

Bahassi, El Mustapha; Yin, Moying; Robbins, Susan B; Li, Ya-Qin; Conrady, Deborah G; Yuan, Zhenyu; Kovall, Rhett A; Herr, Andrew B; Stambrook, Peter J (2011. ) A human cancer-predisposing polymorphism in Cdc25A is embryonic lethal in the mouse and promotes ASK-1 mediated apoptosis.Cell division, , 6 (1 ) ,4

Herr, Andrew B; Conrady, Deborah G (2011. ) Thermodynamic analysis of metal ion-induced protein assembly.Methods in enzymology, , 488 ,101-21

Conrady, Deborah G; Flagler, Michael J; Friedmann, David R; Vander Wielen, Bradley D; Kovall, Rhett A; Weiss, Alison A; Herr, Andrew B (2010. ) Molecular basis of differential B-pentamer stability of Shiga toxins 1 and 2.PloS one, , 5 (12 ) ,e15153

Lu, Cheng; Xu, Hengyu; Ranjith-Kumar, C T; Brooks, Monica T; Hou, Tim Y; Hu, Fuqu; Herr, Andrew B; Strong, Roland K; Kao, C Cheng; Li, Pingwei (2010. ) The structural basis of 5' triphosphate double-stranded RNA recognition by RIG-I C-terminal domain.Structure (London, England : 1993), , 18 (8 ) ,1032-43

Millen, Scott H; Lewallen, Daniel M; Herr, Andrew B; Iyer, Suri S; Weiss, Alison A (2010. ) Identification and characterization of the carbohydrate ligands recognized by pertussis toxin via a glycan microarray and surface plasmon resonance.Biochemistry, , 49 (28 ) ,5954-67

Gomes, Michelle M; Suzuki, Hitoshi; Brooks, Monica T; Tomana, Milan; Moldoveanu, Zina; Mestecky, Jiri; Julian, Bruce A; Novak, Jan; Herr, Andrew B (2010. ) Recognition of galactose-deficient O-glycans in the hinge region of IgA1 by N-acetylgalactosamine-specific snail lectins: a comparative binding study.Biochemistry, , 49 (27 ) ,5671-82

Rajan, Sudarsan; Jagatheesan, Ganapathy; Karam, Chehade N; Alves, Marco L; Bodi, Ilona; Schwartz, Arnold; Bulcao, Christian F; D'Souza, Karen M; Akhter, Shahab A; Boivin, Greg P; Dube, Dipak K; Petrashevskaya, Natalia; Herr, Andrew B; Hullin, Roger; Liggett, Stephen B; Wolska, Beata M; Solaro, R John; Wieczorek, David F (2010. ) Molecular and functional characterization of a novel cardiac-specific human tropomyosin isoform.Circulation, , 121 (3 ) ,410-8

Hennigan, Robert F; Foster, Lauren A; Chaiken, Mary F; Mani, Timmy; Gomes, Michelle M; Herr, Andrew B; Ip, Wallace (2010. ) Fluorescence resonance energy transfer analysis of merlin conformational changes.Molecular and cellular biology, , 30 (1 ) ,54-67

Okano-Kosugi, Hitomi; Matsushita, Osamu; Asada, Shinichi; Herr, Andrew B; Kitagawa, Kouki; Koide, Takaki (2009. ) Development of a high-throughput screening system for the compounds that inhibit collagen-protein interactions.Analytical biochemistry, , 394 (1 ) ,125-31

Herr, A B (2009. ) Direct evidence of a native GPVI dimer at the platelet surface.Journal of thrombosis and haemostasis : JTH, , 7 (8 ) ,1344-6

Herr, Andrew B; Farndale, Richard W (2009. ) Structural insights into the interactions between platelet receptors and fibrillar collagen.The Journal of biological chemistry, , 284 (30 ) ,19781-5

Li, Xiaojun; Ranjith-Kumar, C T; Brooks, Monica T; Dharmaiah, S; Herr, Andrew B; Kao, Cheng; Li, Pingwei (2009. ) The RIG-I-like receptor LGP2 recognizes the termini of double-stranded RNA.The Journal of biological chemistry, , 284 (20 ) ,13881-91

Zhang, Jinjin; Xing, Xu; Herr, Andrew B; Bell, Charles E (2009. ) Crystal structure of E. coli RecE protein reveals a toroidal tetramer for processing double-stranded DNA breaks.Structure (London, England : 1993), , 17 (5 ) ,690-702

Horii, Katsunori; Brooks, Monica T; Herr, Andrew B (2009. ) Convulxin forms a dimer in solution and can bind eight copies of glycoprotein VI: implications for platelet activation.Biochemistry, , 48 (13 ) ,2907-14

Hassett, Daniel J; Sutton, Mark D; Schurr, Michael J; Herr, Andrew B; Caldwell, Charles C; Matu, Joseph O (2009. ) Pseudomonas aeruginosa hypoxic or anaerobic biofilm infections within cystic fibrosis airways.Trends in microbiology, , 17 (3 ) ,130-8

Urrea Moreno, Ramon; Gil, Juana; Rodriguez-Sainz, Carmen; Cela, Elena; LaFay, Victor; Oloizia, Brian; Herr, Andrew B; Sumegi, Janos; Jordan, Michael B; Risma, Kimberly A (2009. ) Functional assessment of perforin C2 domain mutations illustrates the critical role for calcium-dependent lipid binding in perforin cytotoxic function.Blood, , 113 (2 ) ,338-46

Conrady, Deborah G; Brescia, Cristin C; Horii, Katsunori; Weiss, Alison A; Hassett, Daniel J; Herr, Andrew B (2008. ) A zinc-dependent adhesion module is responsible for intercellular adhesion in staphylococcal biofilms.Proceedings of the National Academy of Sciences of the United States of America, , 105 (49 ) ,19456-61

Xu, Zhen; Page, Richard C; Gomes, Michelle M; Kohli, Ekta; Nix, Jay C; Herr, Andrew B; Patterson, Cam; Misra, Saurav (2008. ) Structural basis of nucleotide exchange and client binding by the Hsp70 cochaperone Bag2.Nature structural & molecular biology, , 15 (12 ) ,1309-17

Gomes, Michelle M; Wall, Stephanie B; Takahashi, Kazuo; Novak, Jan; Renfrow, Matthew B; Herr, Andrew B (2008. ) Analysis of IgA1 N-glycosylation and its contribution to FcalphaRI binding.Biochemistry, , 47 (43 ) ,11285-99

Jing, Peng; Rudra, Jai S; Herr, Andrew B; Collier, Joel H (2008. ) Self-assembling peptide-polymer hydrogels designed from the coiled coil region of fibrin.Biomacromolecules, , 9 (9 ) ,2438-46

Yang, Mingyan; Horii, Katsunori; Herr, Andrew B; Kirley, Terence L (2008. ) Characterization and importance of the dimer interface of human calcium-activated nucleotidase.Biochemistry, , 47 (2 ) ,771-8

Yoon, Sang Sun; Karabulut, Ahmet C; Lipscomb, John D; Hennigan, Robert F; Lymar, Sergei V; Groce, Stephanie L; Herr, Andrew B; Howell, Michael L; Kiley, Patricia J; Schurr, Michael J; Gaston, Benjamin; Choi, Kyoung-Hee; Schweizer, Herbert P; Hassett, Daniel J (2007. ) Two-pronged survival strategy for the major cystic fibrosis pathogen, Pseudomonas aeruginosa, lacking the capacity to degrade nitric oxide during anaerobic respiration.The EMBO journal, , 26 (15 ) ,3662-72

Smethurst, Peter A; Onley, David J; Jarvis, Gavin E; O'Connor, Marie N; Knight, C Graham; Herr, Andrew B; Ouwehand, Willem H; Farndale, Richard W (2007. ) Structural basis for the platelet-collagen interaction: the smallest motif within collagen that recognizes and activates platelet Glycoprotein VI contains two glycine-proline-hydroxyproline triplets.The Journal of biological chemistry, , 282 (2 ) ,1296-304

Gomes, Michelle M; Herr, Andrew B (2006. ) IgA and IgA-specific receptors in human disease: structural and functional insights into pathogenesis and therapeutic potential.Springer seminars in immunopathology, , 28 (4 ) ,383-95

O'Connor, Marie N; Smethurst, Peter A; Davies, Lorna W; Joutsi-Korhonen, Lotta; Onley, David J; Herr, Andrew B; Farndale, Richard W; Ouwehand, Willem H (2006. ) Selective blockade of glycoprotein VI clustering on collagen helices.The Journal of biological chemistry, , 281 (44 ) ,33505-10

Yang, Mingyan; Horii, Katsunori; Herr, Andrew B; Kirley, Terence L (2006. ) Calcium-dependent dimerization of human soluble calcium activated nucleotidase: characterization of the dimer interface.The Journal of biological chemistry, , 281 (38 ) ,28307-17

Horii, Katsunori; Kahn, Mark L; Herr, Andrew B (2006. ) Structural basis for platelet collagen responses by the immune-type receptor glycoprotein VI.Blood, , 108 (3 ) ,936-42

Hamburger, A E; Bjorkman, P J; Herr, A B (2006. ) Structural insights into antibody-mediated mucosal immunity. Current topics in microbiology and immunology, , 308 ,173-204

West, Anthony P; Herr, Andrew B; Bjorkman, Pamela J (2004. ) The chicken yolk sac IgY receptor, a functional equivalent of the mammalian MHC-related Fc receptor, is a phospholipase A2 receptor homolog. Immunity, , 20 (5 ) ,601-10

Herr, Andrew B; Ballister, Edward R; Bjorkman, Pamela J (2003. ) Insights into IgA-mediated immune responses from the crystal structures of human FcalphaRI and its complex with IgA1-Fc.Nature, , 423 (6940 ) ,614-20

Herr, Andrew B; White, Clinton L; Milburn, Christina; Wu, Carol; Bjorkman, Pamela J (2003. ) Bivalent binding of IgA1 to FcalphaRI suggests a mechanism for cytokine activation of IgA phagocytosis. Journal of molecular biology, , 327 (3 ) ,645-57

Bennett, Melanie J; Huey-Tubman, Kathryn E; Herr, Andrew B; West, Anthony P; Ross, Scott A; Bjorkman, Pamela J (2002. ) A linear lattice model for polyglutamine in CAG-expansion diseases.Proceedings of the National Academy of Sciences of the United States of America, , 99 (18 ) ,11634-9

West, A P; Giannetti, A M; Herr, A B; Bennett, M J; Nangiana, J S; Pierce, J R; Weiner, L P; Snow, P M; Bjorkman, P J (2001. ) Mutational analysis of the transferrin receptor reveals overlapping HFE and transferrin binding sites.Journal of molecular biology, , 313 (2 ) ,385-97

Yu, K; Herr, A B; Waksman, G; Ornitz, D M (2000. ) Loss of fibroblast growth factor receptor 2 ligand-binding specificity in Apert syndrome.Proceedings of the National Academy of Sciences of the United States of America, , 97 (26 ) ,14536-41

Yeo, H J; Savvides, S N; Herr, A B; Lanka, E; Waksman, G (2000. ) Crystal structure of the hexameric traffic ATPase of the Helicobacter pylori type IV secretion system. Molecular cell, , 6 (6 ) ,1461-72

Waksman, G; Herr, A B (1998. ) New insights into heparin-induced FGF oligomerization.Nature structural biology, , 5 (7 ) ,527-30

Herr, A B; Ornitz, D M; Sasisekharan, R; Venkataraman, G; Waksman, G (1997. ) Heparin-induced self-association of fibroblast growth factor-2. Evidence for two oligomerization processes. The Journal of biological chemistry, , 272 (26 ) ,16382-9

Venkataraman, G; Sasisekharan, V; Herr, A B; Ornitz, D M; Waksman, G; Cooney, C L; Langer, R; Sasisekharan, R (1996. ) Preferential self-association of basic fibroblast growth factor is stabilized by heparin during receptor dimerization and activation. Proceedings of the National Academy of Sciences of the United States of America, , 93 (2 ) ,845-50

Ornitz, D M; Herr, A B; Nilsson, M; Westman, J; Svahn, C M; Waksman, G (1995. ) FGF binding and FGF receptor activation by synthetic heparan-derived di- and trisaccharides. Science (New York, N.Y.), , 268 (5209 ) ,432-6

Li, Xin; Shu, Chang; Yi, Guanghui; Chaton, Catherine T; Shelton, Catherine L; Diao, Jiasheng; Zuo, Xiaobing; Kao, C Cheng; Herr, Andrew B; Li, Pingwei (2013. ) Cyclic GMP-AMP synthase is activated by double-stranded DNA-induced oligomerization.Immunity, , 39 (6 ) ,1019-31

Kachapati, Kritika; Bednar, Kyle J; Adams, David E; Wu, Yuehong; Mittler, Robert S; Jordan, Michael B; Hinerman, Jennifer M; Herr, Andrew B; Ridgway, William M (2013. ) Recombinant soluble CD137 prevents type one diabetes in nonobese diabetic mice.Journal of autoimmunity, , 47 ,94-103

Shu, Chang; Sankaran, Banumathi; Chaton, Catherine T; Herr, Andrew B; Mishra, Ashutosh; Peng, Junmin; Li, Pingwei (2013. ) Structural insights into the functions of TBK1 in innate antimicrobial immunity.Structure (London, England : 1993), , 21 (7 ) ,1137-48

Conrady, Deborah G; Wilson, Jeffrey J; Herr, Andrew B (2013. ) Structural basis for Zn2+-dependent intercellular adhesion in staphylococcal biofilms.Proceedings of the National Academy of Sciences of the United States of America, , 110 (3 ) ,E202-11

RT Strait, MT Posgai, A Mahler, N Barasa, CO Jacob, J Köhl, M Ehlers, K Stringer, SK Shanmukappa, D Witte, MM Hossain, M Khodoun, AB Herr, and FD Finkelman (2014. ) IgG1 protects against renal disease in a mouse model of cryoglobulinemia .Nature, ,

Honors and Awards

2003 Ohio Eminent Scholar, University of Cincinnati

2004 Leukemia Research Foundation Young Investigator Award

2004 V Scholar Award, The V Foundation for Cancer Research

2007 Selected as one of Ohio Bioscience "30 in their 30s", BioOhio

2014 Emerging Entrepreneurial Achievement Award University of Cincinnati

Post Graduate Training and Education

2000-2003 Damon Runyon Research Fellow, California Institute of Technology, , Pasadena, CA

Keywords

X-Ray Crystallography,Biophysical Chemistry,Bacterial Pathogenesis, Receptor Signaling

Patents and Inventions

#8,084,577 Crystal of human glycoprotein VI collagen binding domain Herr, AB and K Horii, Awarded 12-27-2011 Co-Inventor

Use of Zinc Chelators to Inhibit Biofilm Formation AB Herr, DG Conrady, CC Brescia, and SL Ward, Filed Co-Inventor

Topical Compositions Comprising a Zinc Chelator and Gentamicin and Their Methods of Use AB Herr et al., Filed Co-Inventor

Contact Information

Academic - Cardiovascular Research Center
Room 5926
Cincinnati  Ohio, 45267
Phone: 513-558-2333
andrew.herr@uc.edu