David  Hildeman

David Hildeman , PhD


Director of the Immunobiology Graduate Program

Office S5.214 CCHMC
3333 Burnet Avenue
Cincinnati, Ohio 45229
Phone (513)636-0254
Email david.hildeman@cchmc.org


Ph.D.: University of Wisconsin Madison, 1998 (Medical Microbiology and Immunology)

Post Doc: National Jewish Hospital and Medical Center Denver, 2002 (Immunology)

Research and Practice Interests

The overall goal of my research program is to investigate the apoptotic control of T cell homeostasis in health and disease. I have been studying T cell homeostasis for over 15 years.  I have broad training in Immunology, having completed my PhD in Medical Microbiology and Immunology at the University of Wisconsin-Madison.  I then garnered a Howard Hughes Postdoctoral Fellowship in the Laboratory of John Kappler and Philippa Marrack in Denver, CO.  After starting my own lab, I have maintained NIH funding, obtained tenure, and  have trained graduate students and post-doctoral fellows in my laboratory, having had 7 students and fellows in my lab over the past ten years.  Together, my students have won several awards, have published several manuscripts, and have given talks at National Meetings.  As current Director of the Immunobiology Graduate Program, I have a strong interest in and commitment to Graduate Education.  Further, I have served as an ad-hoc member of several study sections, including two study sections on aging and am now a permanent member of the Cellular and Molecular Immunology-B study section.  I have also successfully managed research projects and personnel which has led to additional funding and my lab has published several publications (>40 manuscripts over the past 8 years).  In summary, I have a demonstrated track record of a vigorous and productive research program in an area of high importance to T cell Immunology. 


Peer Reviewed Publications

Currier, Mark A; Eshun, Francis K; Sholl, Allyson; Chernoguz, Artur; Crawford, Kelly; Divanovic, Senad; Boon, Louis; Goins, William F; Frischer, Jason S; Collins, Margaret H; Leddon, Jennifer L; Baird, William H; Haseley, Amy; Streby, Keri A; Wang, Pin-Yi; Hendrickson, Brett W; Brekken, Rolf A; Kaur, Balveen; Hildeman, David; Cripe, Timothy P (2013. ) VEGF blockade enables oncolytic cancer virotherapy in part by modulating intratumoral myeloid cells.Molecular therapy : the journal of the American Society of Gene Therapy, , 21 (5 ) ,1014-23 More Information

Sena, Laura A; Li, Sha; Jairaman, Amit; Prakriya, Murali; Ezponda, Teresa; Hildeman, David A; Wang, Chyung-Ru; Schumacker, Paul T; Licht, Jonathan D; Perlman, Harris; Bryce, Paul J; Chandel, Navdeep S (2013. ) Mitochondria are required for antigen-specific T cell activation through reactive oxygen species signaling.Immunity, , 38 (2 ) ,225-36 More Information

Kurtulus, Sema; Hildeman, David (2013. ) Assessment of CD4(+) and CD8 (+) T cell responses using MHC class I and II tetramers.Methods in molecular biology (Clifton, N.J.), , 979 ,71-9 More Information

Raynor, Jana; Lages, Celine S; Shehata, Hesham; Hildeman, David A; Chougnet, Claire A (2012. ) Homeostasis and function of regulatory T cells in aging.Current opinion in immunology, , 24 (4 ) ,482-7 More Information

Kurtulus, Sema; Tripathi, Pulak; Hildeman, David A (2012. ) Protecting and rescuing the effectors: roles of differentiation and survival in the control of memory T cell development.Frontiers in immunology, , 3 ,404 More Information

Kurtulus, Sema; Tripathi, Pulak; Moreno-Fernandez, Maria E; Sholl, Allyson; Katz, Jonathan D; Grimes, H Leighton; Hildeman, David A (2011. ) Bcl-2 allows effector and memory CD8+ T cells to tolerate higher expression of Bim.Journal of immunology (Baltimore, Md. : 1950), , 186 (10 ) ,5729-37 More Information

Guo, Fukun; Zhang, Shuangmin; Tripathi, Pulak; Mattner, Jochen; Phelan, James; Sproles, Alyssa; Mo, Jun; Wills-Karp, Marsha; Grimes, H Leighton; Hildeman, David; Zheng, Yi (2011. ) Distinct roles of Cdc42 in thymopoiesis and effector and memory T cell differentiation.PloS one, , 6 (3 ) ,e18002 More Information

Doetschman, Thomas; Sholl, Allyson; Chen, Hwu dau rw; Gard, Connie; Hildeman, David A; Bommireddy, Ramireddy (2011. ) Divergent effects of calcineurin A? on regulatory and conventional T-cell homeostasis.Clinical immunology (Orlando, Fla.), , 138 (3 ) ,321-30 More Information

Chougnet, Claire A; Tripathi, Pulak; Lages, Celine S; Raynor, Jana; Sholl, Allyson; Fink, Pamela; Plas, David R; Hildeman, David A (2011. ) A major role for Bim in regulatory T cell homeostasis.Journal of immunology (Baltimore, Md. : 1950), , 186 (1 ) ,156-63 More Information

Chae, Hee-Don; Siefring, Jamie E; Hildeman, David A; Gu, Yi; Williams, David A (2010. ) RhoH regulates subcellular localization of ZAP-70 and Lck in T cell receptor signaling.PloS one, , 5 (11 ) ,e13970 More Information

Rudra, Jai S; Tripathi, Pulak K; Hildeman, David A; Jung, Jangwook P; Collier, Joel H (2010. ) Immune responses to coiled coil supramolecular biomaterials.Biomaterials, , 31 (32 ) ,8475-83 More Information

Kasten, Kevin R; Prakash, Priya S; Unsinger, Jacqueline; Goetzman, Holly S; England, Lisa G; Cave, Cindy M; Seitz, Aaron P; Mazuski, Cristina N; Zhou, Tony T; Morre, Michel; Hotchkiss, Richard S; Hildeman, David A; Caldwell, Charles C (2010. ) Interleukin-7 (IL-7) treatment accelerates neutrophil recruitment through gamma delta T-cell IL-17 production in a murine model of sepsis.Infection and immunity, , 78 (11 ) ,4714-22 More Information

Guo, Fukun; Hildeman, David; Tripathi, Pulak; Velu, Chinavenmeni S; Grimes, H Leighton; Zheng, Yi (2010. ) Coordination of IL-7 receptor and T-cell receptor signaling by cell-division cycle 42 in T-cell homeostasis.Proceedings of the National Academy of Sciences of the United States of America, , 107 (43 ) ,18505-10 More Information

Kasten, Kevin R; Tschöp, Johannes; Goetzman, Holly S; England, Lisa G; Dattilo, Jonathan R; Cave, Cindy M; Seitz, Aaron P; Hildeman, David A; Caldwell, Charles C (2010. ) T-cell activation differentially mediates the host response to sepsis.Shock (Augusta, Ga.), , 34 (4 ) ,377-83 More Information

Kasten, Kevin R; Tschöp, Johannes; Adediran, Samuel G; Hildeman, David A; Caldwell, Charles C (2010. ) T cells are potent early mediators of the host response to sepsis.Shock (Augusta, Ga.), , 34 (4 ) ,327-36 More Information

Lin, Adora A; Wojciechowski, Sara E; Hildeman, David A (2010. ) Androgens suppress antigen-specific T cell responses and IFN-? production during intracranial LCMV infection.Journal of neuroimmunology, , 226 (1-2 ) ,8-19 More Information

Tripathi, Pulak; Kurtulus, Sema; Wojciechowski, Sara; Sholl, Allyson; Hoebe, Kasper; Morris, Suzanne C; Finkelman, Fred D; Grimes, H Leighton; Hildeman, David A (2010. ) STAT5 is critical to maintain effector CD8+ T cell responses.Journal of immunology (Baltimore, Md. : 1950), , 185 (4 ) ,2116-24 More Information

Kurtulus, Sema; Tripathi, Pulak; Opferman, Joseph T; Hildeman, David A (2010. ) Contracting the 'mus cells'--does down-sizing suit us for diving into the memory pool?.Immunological reviews, , 236 ,54-67 More Information

Unsinger, Jacqueline; McGlynn, Margaret; Kasten, Kevin R; Hoekzema, Andrew S; Watanabe, Eizo; Muenzer, Jared T; McDonough, Jacquelyn S; Tschoep, Johannes; Ferguson, Thomas A; McDunn, Jonathan E; Morre, Michel; Hildeman, David A; Caldwell, Charles C; Hotchkiss, Richard S (2010. ) IL-7 promotes T cell viability, trafficking, and functionality and improves survival in sepsis.Journal of immunology (Baltimore, Md. : 1950), , 184 (7 ) ,3768-79 More Information

Barnes, Michael J; Aksoylar, Halil; Krebs, Philippe; Bourdeau, Tristan; Arnold, Carrie N; Xia, Yu; Khovananth, Kevin; Engel, Isaac; Sovath, Sosathya; Lampe, Kristin; Laws, Eleana; Saunders, Amy; Butcher, Geoffrey W; Kronenberg, Mitchell; Steinbrecher, Kris; Hildeman, David; Grimes, H Leighton; Beutler, Bruce; Hoebe, Kasper (2010. ) Loss of T cell and B cell quiescence precedes the onset of microbial flora-dependent wasting disease and intestinal inflammation in Gimap5-deficient mice.Journal of immunology (Baltimore, Md. : 1950), , 184 (7 ) ,3743-54 More Information

Lin, Adora A; Tripathi, Pulak K; Sholl, Allyson; Jordan, Michael B; Hildeman, David A (2009. ) Gamma interferon signaling in macrophage lineage cells regulates central nervous system inflammation and chemokine production.Journal of virology, , 83 (17 ) ,8604-15 More Information

Madan, Rajat; Demircik, Filiz; Surianarayanan, Sangeetha; Allen, Jessica L; Divanovic, Senad; Trompette, Aurelien; Yogev, Nir; Gu, Yuanyuan; Khodoun, Marat; Hildeman, David; Boespflug, Nicholas; Fogolin, Mariela B; Gröbe, Lothar; Greweling, Marina; Finkelman, Fred D; Cardin, Rhonda; Mohrs, Markus; Müller, Werner; Waisman, Ari; Roers, Axel; Karp, Christopher L (2009. ) Nonredundant roles for B cell-derived IL-10 in immune counter-regulation.Journal of immunology (Baltimore, Md. : 1950), , 183 (4 ) ,2312-20 More Information

Morris, Suzanne C; Heidorn, Stephanie M; Herbert, De'Broski R; Perkins, Charles; Hildeman, David A; Khodoun, Marat V; Finkelman, Fred D (2009. ) Endogenously produced IL-4 nonredundantly stimulates CD8+ T cell proliferation. Journal of immunology (Baltimore, Md. : 1950), , 182 (3 ) ,1429-38

Hildeman, David; Janssen, Edith (2008. ) IFN-gamma and self-absorbed CD4+ T cells: a regulatory double negative.Nature immunology, , 9 (11 ) ,1210-2 More Information

Guo, Fukun; Cancelas, Jose A; Hildeman, David; Williams, David A; Zheng, Yi (2008. ) Rac GTPase isoforms Rac1 and Rac2 play a redundant and crucial role in T-cell development.Blood, , 112 (5 ) ,1767-75 More Information

Lages, Celine S; Suffia, Isabelle; Velilla, Paula A; Huang, Bin; Warshaw, Gregg; Hildeman, David A; Belkaid, Yasmin; Chougnet, Claire (2008. ) Functional regulatory T cells accumulate in aged hosts and promote chronic infectious disease reactivation. Journal of immunology (Baltimore, Md. : 1950), , 181 (3 ) ,1835-48

Machado, Fabiana S; Esper, Lísia; Dias, Alexandra; Madan, Rajat; Gu, YuanYuan; Hildeman, David; Serhan, Charles N; Karp, Christopher L; Aliberti, Júlio (2008. ) Native and aspirin-triggered lipoxins control innate immunity by inducing proteasomal degradation of TRAF6.The Journal of experimental medicine, , 205 (5 ) ,1077-86 More Information

Zarebski, Adrian; Velu, Chinavenmeni S; Baktula, Avinash M; Bourdeau, Tristan; Horman, Shane R; Basu, Sudeep; Bertolone, Salvatore J; Horwitz, Marshal; Hildeman, David A; Trent, John O; Grimes, H Leighton (2008. ) Mutations in growth factor independent-1 associated with human neutropenia block murine granulopoiesis through colony stimulating factor-1.Immunity, , 28 (3 ) ,370-80 More Information

Reckling, Stacie; Divanovic, Senad; Karp, Christopher L; Wojciechowski, Sara; Belkaid, Yasmine; Hildeman, David (2008. ) Proapoptotic Bcl-2 family member Bim promotes persistent infection and limits protective immunity.Infection and immunity, , 76 (3 ) ,1179-85 More Information

Hildeman, David; Jorgensen, Trine; Kappler, John; Marrack, Philippa (2007. ) Apoptosis and the homeostatic control of immune responses.Current opinion in immunology, , 19 (5 ) ,516-21 More Information

Wojciechowski, Sara; Tripathi, Pulak; Bourdeau, Tristan; Acero, Luis; Grimes, H Leighton; Katz, Jonathan D; Finkelman, Fred D; Hildeman, David A (2007. ) Bim/Bcl-2 balance is critical for maintaining naive and memory T cell homeostasis.The Journal of experimental medicine, , 204 (7 ) ,1665-75 More Information

DeKoter, Rodney P; Schweitzer, Brock L; Kamath, Meghana B; Jones, Darrel; Tagoh, Hiromi; Bonifer, Constanze; Hildeman, David A; Huang, Kelly J (2007. ) Regulation of the interleukin-7 receptor alpha promoter by the Ets transcription factors PU.1 and GA-binding protein in developing B cells.The Journal of biological chemistry, , 282 (19 ) ,14194-204 More Information

Chougnet, Claire; Hildeman, David A (2007. ) It's hard to get downstream without a raft: a commentary on "reactive oxygen species promote raft formation in T lymphocytes".Free radical biology & medicine, , 42 (7 ) ,933-5 More Information

Tripathi, Pulak; Mitchell, Thomas C; Finkelman, Fred; Hildeman, David A (2007. ) Cutting Edge: Limiting amounts of IL-7 do not control contraction of CD4+ T cell responses. Journal of immunology (Baltimore, Md. : 1950), , 178 (7 ) ,4027-31

Gu, Yi; Chae, Hee-Don; Siefring, Jamie E; Jasti, Aparna C; Hildeman, David A; Williams, David A (2006. ) RhoH GTPase recruits and activates Zap70 required for T cell receptor signaling and thymocyte development.Nature immunology, , 7 (11 ) ,1182-90 More Information

Grayson, Jason M; Weant, Ashley E; Holbrook, Beth C; Hildeman, David (2006. ) Role of Bim in regulating CD8+ T-cell responses during chronic viral infection.Journal of virology, , 80 (17 ) ,8627-38 More Information

Wojciechowski, Sara; Jordan, Michael B; Zhu, Yanan; White, Janice; Zajac, Allan J; Hildeman, David A (2006. ) Bim mediates apoptosis of CD127(lo) effector T cells and limits T cell memory.European journal of immunology, , 36 (7 ) ,1694-706 More Information

Zhu, Yanan; Liu, Xinqi; Hildeman, David; Peyerl, Fred W; White, Janice; Kushnir, Elenora; Kappler, John; Marrack, Philippa (2006. ) Bax does not have to adopt its final form to drive T cell death.The Journal of experimental medicine, , 203 (5 ) ,1147-52 More Information

Tripathi, Pulak; Madan, Rajat; Chougnet, Claire; Divanovic, Senad; Ma, Xiaojing; Wahl, Larry M; Gajewski, Thomas; Karp, Christopher L; Hildeman, David A (2006. ) An adenoviral vector for probing promoter activity in primary immune cells.Journal of immunological methods, , 311 (1-2 ) ,19-30 More Information

Fuller, Michael J; Hildeman, David A; Sabbaj, Steffanie; Gaddis, Dalia E; Tebo, Anne E; Shang, Liang; Goepfert, Paul A; Zajac, Allan J (2005. ) Cutting edge: emergence of CD127high functionally competent memory T cells is compromised by high viral loads and inadequate T cell help. Journal of immunology (Baltimore, Md. : 1950), , 174 (10 ) ,5926-30

Hawlisch, Heiko; Belkaid, Yasmine; Baelder, Ralf; Hildeman, David; Gerard, Craig; Köhl, Jörg (2005. ) C5a negatively regulates toll-like receptor 4-induced immune responses.Immunity, , 22 (4 ) ,415-26 More Information

Jordan, Michael B; Hildeman, David; Kappler, John; Marrack, Philippa (2004. ) An animal model of hemophagocytic lymphohistiocytosis (HLH): CD8+ T cells and interferon gamma are essential for the disorder.Blood, , 104 (3 ) ,735-43 More Information

Hildeman, David A (2004. ) Regulation of T-cell apoptosis by reactive oxygen species.Free radical biology & medicine, , 36 (12 ) ,1496-504 More Information

Zhu, Yanan; Swanson, Bradley J; Wang, Michael; Hildeman, David A; Schaefer, Brian C; Liu, Xinqi; Suzuki, Hiroyuki; Mihara, Katsuyoshi; Kappler, John; Marrack, Philippa (2004. ) Constitutive association of the proapoptotic protein Bim with Bcl-2-related proteins on mitochondria in T cells.Proceedings of the National Academy of Sciences of the United States of America, , 101 (20 ) ,7681-6 More Information

Gardai, Shyra J; Hildeman, David A; Frankel, Steve K; Whitlock, Ben B; Frasch, S Courtney; Borregaard, Niels; Marrack, Philippa; Bratton, Donna L; Henson, Peter M (2004. ) Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils.The Journal of biological chemistry, , 279 (20 ) ,21085-95 More Information

Zhang, Rui; Fichtenbaum, Carl J; Hildeman, David A; Lifson, Jeffrey D; Chougnet, Claire (2004. ) CD40 ligand dysregulation in HIV infection: HIV glycoprotein 120 inhibits signaling cascades upstream of CD40 ligand transcription. Journal of immunology (Baltimore, Md. : 1950), , 172 (4 ) ,2678-86

Ivanovska, Iva; Galonek, Heidi L; Hildeman, David A; Hardwick, J Marie (2004. ) Regulation of cell death in the lymphoid system by Bcl-2 family proteins.Acta haematologica, , 111 (1-2 ) ,42-55 More Information

Hildeman, David A; Mitchell, Thomas; Aronow, Bruce; Wojciechowski, Sara; Kappler, John; Marrack, Philippa (2003. ) Control of Bcl-2 expression by reactive oxygen species.Proceedings of the National Academy of Sciences of the United States of America, , 100 (25 ) ,15035-40 More Information

Hildeman, David A; Mitchell, Thomas; Kappler, John; Marrack, Philippa (2003. ) T cell apoptosis and reactive oxygen species.The Journal of clinical investigation, , 111 (5 ) ,575-81 More Information

Mitchell, Thomas C; Teague, T Kent; Hildeman, David A; Bender, Jeremy; Rees, William A; Kedl, Ross M; Swanson, Brad; Kappler, John W; Marrack, Philippa (2002. ) Stronger correlation of bcl-3 than bcl-2, bcl-xL, costimulation, or antioxidants with adjuvant-induced T cell survival. Annals of the New York Academy of Sciences, , 975 ,114-31

Hildeman, David A; Zhu, Yanan; Mitchell, Thomas C; Kappler, John; Marrack, Philippa (2002. ) Molecular mechanisms of activated T cell death in vivo. Current opinion in immunology, , 14 (3 ) ,354-9

Hildeman, David A; Zhu, Yanan; Mitchell, Thomas C; Bouillet, Philippe; Strasser, Andreas; Kappler, John; Marrack, Philippa (2002. ) Activated T cell death in vivo mediated by proapoptotic bcl-2 family member bim. Immunity, , 16 (6 ) ,759-67

Raynor, Jana; Karns, Rebekah; Almanan, Maha; Li, Kun-Po; Divanovic, Senad; Chougnet, Claire A; Hildeman, David A (2015. ) IL-6 and ICOS Antagonize Bim and Promote Regulatory T Cell Accrual with Age.Journal of immunology (Baltimore, Md. : 1950), , 195 (3 ) ,944-52 More Information

Lampe, Kristin; Endale, Mehari; Cashman, Siobhan; Fang, Hao; Mattner, Jochen; Hildeman, David; Hoebe, Kasper (2015. ) Slp-76 is a critical determinant of NK-cell mediated recognition of missing-self targets.European journal of immunology, , 45 (7 ) ,2072-83 More Information

Niss, Omar; Sholl, Allyson; Bleesing, Jack J; Hildeman, David A (2015. ) IL-10/Janus kinase/signal transducer and activator of transcription 3 signaling dysregulates Bim expression in autoimmune lymphoproliferative syndrome.The Journal of allergy and clinical immunology, , 135 (3 ) ,762-70 More Information

McNally, Jonathan P; Elfers, Eileen E; Terrell, Catherine E; Grunblatt, Eli; Hildeman, David A; Jordan, Michael B; Katz, Jonathan D (2014. ) Eliminating encephalitogenic T cells without undermining protective immunity.Journal of immunology (Baltimore, Md. : 1950), , 192 (1 ) ,73-83 More Information

Johnson, Theodore S; Terrell, Catherine E; Millen, Scott H; Katz, Jonathan D; Hildeman, David A; Jordan, Michael B (2014. ) Etoposide selectively ablates activated T cells to control the immunoregulatory disorder hemophagocytic lymphohistiocytosis.Journal of immunology (Baltimore, Md. : 1950), , 192 (1 ) ,84-91 More Information

Pierson, Wim; Cauwe, Bénédicte; Policheni, Antonia; Schlenner, Susan M; Franckaert, Dean; Berges, Julien; Humblet-Baron, Stephanie; Schönefeldt, Susann; Herold, Marco J; Hildeman, David; Strasser, Andreas; Bouillet, Philippe; Lu, Li-Fan; Matthys, Patrick; Freitas, Antonio A; Luther, Rita J; Weaver, Casey T; Dooley, James; Gray, Daniel H D; Liston, Adrian (2013. ) Antiapoptotic Mcl-1 is critical for the survival and niche-filling capacity of Foxp3? regulatory T cells.Nature immunology, , 14 (9 ) ,959-65 More Information

Raynor, Jana; Sholl, Allyson; Plas, David R; Bouillet, Philippe; Chougnet, Claire A; Hildeman, David A (2013. ) IL-15 Fosters Age-Driven Regulatory T Cell Accrual in the Face of Declining IL-2 Levels.Frontiers in immunology, , 4 ,161 More Information

Phelan, James D; Saba, Ingrid; Zeng, Hui; Kosan, Christian; Messer, Malynda S; Olsson, H Andre; Fraszczak, Jennifer; Hildeman, David A; Aronow, Bruce J; Möröy, Tarik; Grimes, H Leighton (2013. ) Growth factor independent-1 maintains Notch1-dependent transcriptional programming of lymphoid precursors.PLoS genetics, , 9 (9 ) ,e1003713 More Information

Courses Taught

-IMM-8088 FOUND OF IMMUNOL Level:Graduate

-IMM-8090 ADV TOPICS Level:Graduate

-IMM-8091 ADV TOPICS II Level:Graduate

-IMM-8072 RESEARCH Level:Graduate

Contact Information

Academic - Office S5.214 CCHMC
3333 Burnet Avenue
Cincinnati  Ohio, 45229
Phone: (513)636-0254