Nelson Horseman

Nelson Horseman

Professor Emeritus, Department of Molecular and Cellular Physiology, UC College of Medicine

Medical Sciences Building

4257A

COM PSP Systems Physiology - 0576

Department of Molecular and Cellular Physiology

Professional Summary

Major Field: Endocrinology and Molecular Biology
Responsibilities:  See Sections 7, 8, 9

Research Support

Investigators:Laura Hernandez DOD Breast Cancer Program, Postdoctoral Fellowship Application Involvement of serotonin in breast cancer metastasis to bone. 100,000/yr, 3 years Denied Type:Fellowship

Investigators:Laurie Nommsen-Rivers, P.I; Karen A. Gregerson, co-PI; Nelson D. Horseman, co-PI CSTA T1 Translational grant Anti-depressant therapy in breastfeeding mothers. Role:Co-PI 100,000/yr, 2 yrs Type:Grant

2009 USDA Mechanisms of homeostasis in bovine lactation. 350,000, total (2 year) Active Type:Grant

Investigators:Amy Dugan, PI; Nelson D. Horseman, Co-I Shriners Hospitals for Children Anxiolytic drug effects following burn and psychogenic stress. Role:Co-I Active Type:Grant

Grant: #? Investigators:Karen Gregerson, P.I., N.D. Horseman, Co-I, A.R. Buckley, Co-I 08-2010 -2012 D.O.D CDMRP Breast Cancer Research Program Humanized models for pre-clinical studies of breast cancer. Role:Co-I $150K/annum Pending Type:Grant Level:National

Grant: #1R01CA136801-01 Investigators:Nelson D. Horseman, P.I. NCI Control of Bone Metastasis Role:P.I. Denied Type:Grant Level:National

Grant: #09-01-A0-S2-E2 Investigators:Horseman, Nelson 12-01-2000 -11-30-2001 Charlotte Geyer Foundation Breast Development and Cancer Role:PI $100,000.00 Closed Level:Private Non-Profit

Grant: #R01-DK-52134 Investigators:Horseman, Nelson 07-01-2002 -05-31-2008 National Inst of Diabetes and Digestive and Kidney Disease Genetic and Biochemical Dissection of Prolactin Actions Role:PI $2,179,257.97 Closed Level:Federal

Grant: #5-R01-DK-52134-04-A0-S0-E0 Investigators:Horseman, Nelson 06-01-1998 -06-30-2002 National Inst of Diabetes and Digestive and Kidney Disease Genetic and Biochemical Dissection of Prolactin Actions Role:PI $1,174,515.00 Closed Level:Federal

Investigators:Horseman, Nelson 06-20-1999 -11-05-2001 AviGenics, Incorporated Chicken FABP Promotor Role:PI $135,810.00 Closed Level:Industry

Grant: #2007-35206-17898 Investigators:Horseman, Nelson 02-01-2007 -01-31-2009 Department of Agriculture Mechanisms of Homeostasis in Bovine Lactation Role:PI $333,204.00 Closed Level:Federal

Grant: #W81XWH-06-1-0635 Investigators:Horseman, Nelson 07-17-2006 -08-16-2007 Department of the Army Medical Research Acquisition Activity Evolution of Serotonin Signaling in Breast Cancer Role:PI $115,125.00 Closed Level:Federal

Grant: #Shriners 8520 Investigators:Gregerson, Karen; Horseman, Nelson; Ogle, Cora 01-08-2008 -12-31-2008 Shriners Hospitals for Children - Cincinnati Shrine Physiology 2008 Consulting Agreements Role:PI $247,589.00 Closed Level:Private Non-Profit

Grant: #235-433 Investigators:Horseman, Nelson 07-01-2007 -06-30-2012 Ohio Board of Regents Economic Growth Challenge/Innovation Incentive Program in Translational Application of UC Cardiopulmonary Research Role:PI $1,250,000.00 Active Level:State of Ohio

Grant: #2009-35206-05178 Investigators:Horseman, Nelson 03-01-2009 -02-28-2011 Department of Agriculture Mechanisms of Homeostasis in Bovine Lactation Role:PI $350,000.00 Closed Level:Federal

Grant: #1006754 Investigators:Babcock, George; Dugan, Amy; Horseman, Nelson 01-01-2009 -12-31-2009 Shriners Hospitals for Children - Cincinnati Shrine Physiology 2009 Consulting Agreements Role:Collaborator $175,036.00 Closed Level:Private Non-Profit

Grant: #W81XWH-10-1-0515 Investigators:Buckley, Arthur; Gregerson, Karen; Horseman, Nelson 09-01-2010 -09-30-2012 Department of the Army Human-Compatible Animal Models for Preclinical Research on Hormones in Breast Cancer Role:Collaborator $588,750.00 Closed Level:Federal

Grant: #2001180927 Investigators:Horseman, Nelson 04-18-2011 -04-17-2012 Johns Hopkins University The Regulation of Endocrine Parathyroid Hormone-Related Peptide Role:PI $24,492.00 Closed Level:Institution of Higher Education

Publications

Peer Reviewed Publications

Horseman, Nelson D; Hernandez, Laura L (2014. )New concepts of breast cell communication to bone.Trends in endocrinology and metabolism: TEM, ,25 (1 ),34-41

Horseman, Nelson D; Gregerson, Karen A (2014. )Prolactin actions.Journal of molecular endocrinology, ,52 (1 ),R95-106

Christensen, Heather R; Murawsky, Michael K; Horseman, Nelson D; Willson, Tara A; Gregerson, Karen A (2013. )Completely humanizing prolactin rescues infertility in prolactin knockout mice and leads to human prolactin expression in extrapituitary mouse tissues.Endocrinology, ,154 (12 ),4777-89

Marshall, Aaron M; Hernandez, Laura L; Horseman, Nelson D (2013. )Serotonin and Serotonin Transport in the Regulation of Lactation.Journal of mammary gland biology and neoplasia, ,

Lemay, Danielle G; Ballard, Olivia A; Hughes, Maria A; Morrow, Ardythe L; Horseman, Nelson D; Nommsen-Rivers, Laurie A (2013. )RNA sequencing of the human milk fat layer transcriptome reveals distinct gene expression profiles at three stages of lactation.PloS one, ,8 (7 ),e67531

Chen, Chien-Chung; Stairs, Douglas B; Boxer, Robert B; Belka, George K; Horseman, Nelson D; Alvarez, James V; Chodosh, Lewis A (2012. )Autocrine prolactin induced by the Pten-Akt pathway is required for lactation initiation and provides a direct link between the Akt and Stat5 pathways.Genes & development, ,26 (19 ),2154-68

Collier, R J; Hernandez, L L; Horseman, N D (2012. )Serotonin as a homeostatic regulator of lactation.Domestic animal endocrinology, ,43 (2 ),161-70

Neville, Margaret C; Anderson, Steven M; McManaman, James L; Badger, Thomas M; Bunik, Maya; Contractor, Nikhat; Crume, Tessa; Dabelea, Dana; Donovan, Sharon M; Forman, Nicole; Frank, Daniel N; Friedman, Jacob E; German, J Bruce; Goldman, Armond; Hadsell, Darryl; Hambidge, Michael; Hinde, Katie; Horseman, Nelson D; Hovey, Russell C; Janoff, Edward; Krebs, Nancy F; Lebrilla, Carlito B; Lemay, Danielle G; MacLean, Paul S; Meier, Paula; Morrow, Ardythe L; Neu, Josef; Nommsen-Rivers, Laurie A; Raiten, Daniel J; Rijnkels, Monique; Seewaldt, Victoria; Shur, Barry D; VanHouten, Joshua; Williamson, Peter (2012. )Lactation and neonatal nutrition: defining and refining the critical questions.Journal of mammary gland biology and neoplasia, ,17 (2 ),167-88

Hernandez, Laura L; Gregerson, Karen A; Horseman, Nelson D (2012. )Mammary gland serotonin regulates parathyroid hormone-related protein and other bone-related signals.American journal of physiology. Endocrinology and metabolism, ,302 (8 ),E1009-15

Hernandez, Laura L; Grayson, Bernadette E; Yadav, Ekta; Seeley, Randy J; Horseman, Nelson D (2012. )High fat diet alters lactation outcomes: possible involvement of inflammatory and serotonergic pathways.PloS one, ,7 (3 ),e32598

Hernandez, L L; Collier, J L; Vomachka, A J; Collier, R J; Horseman, N D (2011. )Suppression of lactation and acceleration of involution in the bovine mammary gland by a selective serotonin reuptake inhibitor.The Journal of endocrinology, ,209 (1 ),45-54

Pai, Vaibhav P; Horseman, Nelson D (2011. )Multiple cellular responses to serotonin contribute to epithelial homeostasis.PloS one, ,6 (2 ),e17028

Marshall, Aaron M; Nommsen-Rivers, Laurie A; Hernandez, Laura L; Dewey, Kathryn G; Chantry, Caroline J; Gregerson, Karen A; Horseman, Nelson D (2010. )Serotonin transport and metabolism in the mammary gland modulates secretory activation and involution.The Journal of clinical endocrinology and metabolism, ,95 (2 ),837-46

Dugan, Amy L; Gregerson, Karen A; Neely, Alice; Gardner, Jason; Noel, Greg J; Babcock, George F; Horseman, Nelson D Mice treated with a benzodiazepine had an improved survival rate following Pseudomonas aeruginosa infection.Journal of burn care & research : official publication of the American Burn Association, ,31 (1 ),1-12

Hernandez, Laura L; Limesand, Sean W; Collier, Jayne L; Horseman, Nelson D; Collier, Robert J (2009. )The bovine mammary gland expresses multiple functional isoforms of serotonin receptors.The Journal of endocrinology, ,203 (1 ),123-31

Marshall, Aaron M; Pai, Vaibhav P; Sartor, Maureen A; Horseman, Nelson D (2009. )In vitro multipotent differentiation and barrier function of a human mammary epithelium.Cell and tissue research, ,335 (2 ),383-95

Pai, Vaibhav P; Marshall, Aaron M; Hernandez, Laura L; Buckley, Arthur R; Horseman, Nelson D (2009. )Altered serotonin physiology in human breast cancers favors paradoxical growth and cell survival.Breast cancer research : BCR, ,11 (6 ),R81

Pai, Vaibhav P; Horseman, Nelson D (2008. )Biphasic regulation of mammary epithelial resistance by serotonin through activation of multiple pathways.The Journal of biological chemistry, ,283 (45 ),30901-10

Stull, Malinda A; Pai, Vaibhav; Vomachka, Archie J; Marshall, Aaron M; Jacob, George A; Horseman, Nelson D (2007. )Mammary gland homeostasis employs serotonergic regulation of epithelial tight junctions.Proceedings of the National Academy of Sciences of the United States of America, ,104 (42 ),16708-13

John, S K P; Jones, O M; Horseman, N; Thomas, P; Howell, R D; Fozard, J B J (2007. )Inter general practice variability in use of referral guidelines for colorectal cancer.Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland, ,9 (8 ),731-5

Jones, O M; John, S K P; Horseman, N; Lawrance, R J; Fozard, J B J (2007. )Low anastomotic leak rate after colorectal surgery: a single-centre study.Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland, ,9 (8 ),740-4

Bao, Lei; Tessier, Christian; Prigent-Tessier, Anne; Li, Feixue; Buzzio, Oscar L; Callegari, Eduardo A; Horseman, Nelson D; Gibori, Geula (2007. )Decidual prolactin silences the expression of genes detrimental to pregnancy.Endocrinology, ,148 (5 ),2326-34

Jones, O M; John, S K P; Horseman, N; Lawrance, R J; Fozard, J B J (2007. )Cause and place of death in patients dying with colorectal cancer.Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland, ,9 (3 ),253-7

Soares, Michael J; Alam, S M Khorshed; Duckworth, Mary Lynn; Horseman, Nelson D; Konno, Toshihiro; Linzer, Daniel I H; Maltais, Lois J; Nilsen-Hamilton, Marit; Shiota, Kunio; Smith, Jennifer R; Wallis, Michael (2007. )A standardized nomenclature for the mouse and rat prolactin superfamilies.Mammalian genome : official journal of the International Mammalian Genome Society, ,18 (3 ),154-6

Dugan, Amy L; Schwemberger, Sandy; Noel, Greg J; Babcock, George; Ogle, Cora K; Horseman, Nelson D (2007. )Psychogenic stress prior to burn injury has differential effects on bone marrow and cytokine responses. Experimental biology and medicine (Maywood, N.J.), ,232 (2 ),253-61

Dugan, Amy L; Schwemberger, Sandy; Noel, Greg J; Babcock, George F; Ogle, Cora K; Buckley, Donna J; Horseman, Nelson D; Gregerson, Karen A (2007. )Stress and prolactin effects on bone marrow myeloid cells, serum chemokine and serum glucocorticoid levels in mice.Neuroimmunomodulation, ,14 (6 ),287-96

LaPensee, Christopher R; Horseman, Nelson D; Tso, Patrick; Brandebourg, Terry D; Hugo, Eric R; Ben-Jonathan, Nira (2006. )The prolactin-deficient mouse has an unaltered metabolic phenotype.Endocrinology, ,147 (10 ),4638-45

Obál, Ferenc; Garcia-Garcia, Fabio; Kacsóh, Balint; Taishi, Ping; Bohnet, Stewart; Horseman, Nelson D; Krueger, James M (2005. )Rapid eye movement sleep is reduced in prolactin-deficient mice.The Journal of neuroscience : the official journal of the Society for Neuroscience, ,25 (44 ),10282-9

Dugan, A L; Malarkey, W B; Schwemberger, S; Jauch, E C; Ogle, C K; Horseman, N D Serum levels of prolactin, growth hormone, and cortisol in burn patients: correlations with severity of burn, serum cytokine levels, and fatality. The Journal of burn care & rehabilitation, ,25 (3 ),306-13

Bailey, Jason P; Nieport, Kathryn M; Herbst, Matthew P; Srivastava, Sunil; Serra, Rosa A; Horseman, Nelson D (2004. )Prolactin and transforming growth factor-beta signaling exert opposing effects on mammary gland morphogenesis, involution, and the Akt-forkhead pathway.Molecular endocrinology (Baltimore, Md.), ,18 (5 ),1171-84

Matsuda, Manabu; Imaoka, Tatsuhiko; Vomachka, Archie J; Gudelsky, Gary A; Hou, Zhaoyuan; Mistry, Meenakshi; Bailey, Jason P; Nieport, Kathryn M; Walther, Diego J; Bader, Michael; Horseman, Nelson D (2004. )Serotonin regulates mammary gland development via an autocrine-paracrine loop. Developmental cell, ,6 (2 ),193-203

Dugan, Amy L; Schwemberger, Sandy; Babcock, George F; Buckley, Donna; Buckley, Arthur R; Ogle, Cora K; Horseman, Nelson D (2004. )Effects of prolactin level on burn-induced aberrations in myelopoiesis.Shock (Augusta, Ga.), ,21 (2 ),151-9

Srivastava, Sunil; Matsuda, Manabu; Hou, Zhaoyuan; Bailey, Jason P; Kitazawa, Riko; Herbst, Matthew P; Horseman, Nelson D (2003. )Receptor activator of NF-kappaB ligand induction via Jak2 and Stat5a in mammary epithelial cells.The Journal of biological chemistry, ,278 (46 ),46171-8

Dorshkind, Kenneth; Welniak, Lisbeth; Gault, Ruth A; Hixon, Julie; Montecino-Rodriguez, Encarnacion; Horseman, Nelson D; Gertner, Joseph M; Murphy, William J (2003. )Effects of housing on the thymic deficiency in dwarf mice and its reversal by growth hormone administration. Clinical immunology (Orlando, Fla.), ,109 (2 ),197-202

Hou, Zhaoyuan; Srivastava, Sunil; Mistry, Meenakshi J; Herbst, Matthew P; Bailey, Jason P; Horseman, Nelson D (2003. )Two tandemly linked interferon-gamma-activated sequence elements in the promoter of glycosylation-dependent cell adhesion molecule 1 gene synergistically respond to prolactin in mouse mammary epithelial cells.Molecular endocrinology (Baltimore, Md.), ,17 (10 ),1910-20

Tessier, Christian; Prigent-Tessier, Anne; Bao, Lei; Telleria, Carlos M; Ferguson-Gottschall, Susan; Gibori, Gil B; Gu, Yan; Bowen-Shauver, Jennifer M; Horseman, Nelson D; Gibori, Geula (2003. )Decidual activin: its role in the apoptotic process and its regulation by prolactin.Biology of reproduction, ,68 (5 ),1687-94

Krishnan, Nithya; Thellin, Olivier; Buckley, Donna J; Horseman, Nelson D; Buckley, Arthur R (2003. )Prolactin suppresses glucocorticoid-induced thymocyte apoptosis in vivo.Endocrinology, ,144 (5 ),2102-10

Naylor, Mathew J; Lockefeer, Jason A; Horseman, Nelson D; Ormandy, Christopher J Prolactin regulates mammary epithelial cell proliferation via autocrine/paracrine mechanism.Endocrine, ,20 (1-2 ),111-4

Matsuda, Manabu; Lockefeer, Jason A; Horseman, Nelson D Aldolase C/zebrin gene regulation by prolactin during pregnancy and lactation.Endocrine, ,20 (1-2 ),91-100

Ormandy, Christopher J; Naylor, Matthew; Harris, Jessica; Robertson, Fiona; Horseman, Nelson D; Lindeman, Geoffrey J; Visvader, Jane; Kelly, Paul A (2003. )Investigation of the transcriptional changes underlying functional defects in the mammary glands of prolactin receptor knockout mice. Recent progress in hormone research, ,58 ,297-323

Cruz-Soto, Martha E; Scheiber, Michael D; Gregerson, Karen A; Boivin, Gregory P; Horseman, Nelson D (2002. )Pituitary tumorigenesis in prolactin gene-disrupted mice.Endocrinology, ,143 (11 ),4429-36

Dugan, Amy L; Thellin, Olivier; Buckley, Donna J; Buckley, Arthur R; Ogle, Cora K; Horseman, Nelson D (2002. )Effects of prolactin deficiency on myelopoiesis and splenic T lymphocyte proliferation in thermally injured mice.Endocrinology, ,143 (10 ),4147-51

Imaoka, Tatsuhiko; Horseman, Nelson D; Lockefeer, Jason A; Mori, Takao; Matsuda, Manabu (2002. )Cortactin-binding protein 90 (CBP90) expression in the mouse mammary glands during prolactin-induced lobuloalveolar development. Zoological science, ,19 (4 ),443-8

Horseman, Nelson D (2002. )Prolactin receptor diversity in humans: novel isoforms suggest general principles. Trends in endocrinology and metabolism: TEM, ,13 (2 ),47-8

Thellin, O; Noel, G; Khurana, S; Ogle, C K; Horseman, N D (2001. )Stress hormone secretion and gut signal transducer (STAT) proteins after burn injury in rats. Shock (Augusta, Ga.), ,16 (5 ),393-7

Dorshkind, K; Horseman, N D (2001. )Anterior pituitary hormones, stress, and immune system homeostasis.BioEssays : news and reviews in molecular, cellular and developmental biology, ,23 (3 ),288-94

Hou, Z; Bailey, J P; Vomachka, A J; Matsuda, M; Lockefeer, J A; Horseman, N D (2000. )Glycosylation-dependent cell adhesion molecule 1 (GlyCAM 1) is induced by prolactin and suppressed by progesterone in mammary epithelium.Endocrinology, ,141 (11 ),4278-83

Ogle, C K; Kong, F; Guo, X; Wells, D A; Aosasa, S; Noel, G; Horseman, N (2000. )The effect of burn injury on suppressors of cytokine signalling. Shock (Augusta, Ga.), ,14 (3 ),392-8; discussion 39

Foster, M P; Jensen, E R; Montecino-Rodriguez, E; Leathers, H; Horseman, N; Dorshkind, K (2000. )Humoral and cell-mediated immunity in mice with genetic deficiencies of prolactin, growth hormone, insulin-like growth factor-I, and thyroid hormone.Clinical immunology (Orlando, Fla.), ,96 (2 ),140-9

Phelps, C J; Horseman, N D (2000. )Prolactin gene disruption does not compromise differentiation of tuberoinfundibular dopaminergic neurons.Neuroendocrinology, ,72 (1 ),2-10

Dorshkind, K; Horseman, N D (2000. )The roles of prolactin, growth hormone, insulin-like growth factor-I, and thyroid hormones in lymphocyte development and function: insights from genetic models of hormone and hormone receptor deficiency.Endocrine reviews, ,21 (3 ),292-312

Pratt, S L; Ogle, C K; Mao, J X; Zhao, W; Lovell, G; Horseman, N D (2000. )Interleukin-6 signal transduction in human intestinal epithelial cells. Shock (Augusta, Ga.), ,13 (6 ),435-40

Vomachka, A J; Pratt, S L; Lockefeer, J A; Horseman, N D (2000. )Prolactin gene-disruption arrests mammary gland development and retards T-antigen-induced tumor growth.Oncogene, ,19 (8 ),1077-84

Horseman, N D (1999. )Citation for the 1999 Gerald D. Aurbach Lecture Award of the Endocrine Society to Dr. James E. Darnell. Endocrine reviews, ,20 (4 ),591-2

Gao, J; Horseman, N D (1999. )Prolactin-independent modulation of the beta-casein response element by Erk2 MAP kinase. Cellular signalling, ,11 (3 ),205-10

Horseman, N D (1999. )Prolactin and mammary gland development. Journal of mammary gland biology and neoplasia, ,4 (1 ),79-88

Steger, R W; Chandrashekar, V; Zhao, W; Bartke, A; Horseman, N D (1998. )Neuroendocrine and reproductive functions in male mice with targeted disruption of the prolactin gene.Endocrinology, ,139 (9 ),3691-5

Pratt, S L; Horseman, N D (1998. )Identification of two Y-box binding proteins that interact with the promoters of columbid annexin I genes. Gene, ,214 (1-2 ),147-56

Horseman, N D; Zhao, W; Montecino-Rodriguez, E; Tanaka, M; Nakashima, K; Engle, S J; Smith, F; Markoff, E; Dorshkind, K (1997. )Defective mammopoiesis, but normal hematopoiesis, in mice with a targeted disruption of the prolactin gene.The EMBO journal, ,16 (23 ),6926-35

Schuler, L A; Nagel, R J; Gao, J; Horseman, N D; Kessler, M A (1997. )Prolactin receptor heterogeneity in bovine fetal and maternal tissues.Endocrinology, ,138 (8 ),3187-94

Horseman, N D; Engle, S J; Ralescu, A The logic of signaling from the cell surface to the nucleus. Trends in endocrinology and metabolism: TEM, ,8 (4 ),123-9

Gao, J; Hughes, J P; Auperin, B; Buteau, H; Edery, M; Zhuang, H; Wojchowski, D M; Horseman, N D (1996. )Interactions among Janus kinases and the prolactin (PRL) receptor in the regulation of a PRL response element.Molecular endocrinology (Baltimore, Md.), ,10 (7 ),847-56

Gunteski-Hamblin, A M; Song, G; Walsh, R A; Frenzke, M; Boivin, G P; Dorn, G W; Kaetzel, M A; Horseman, N D; Dedman, J R (1996. )Annexin VI overexpression targeted to heart alters cardiomyocyte function in transgenic mice. The American journal of physiology, ,270 (3 Pt 2 ),H1091-100

Horseman, N D (1995. )Prolactin, proliferation, and protooncogenes.Endocrinology, ,136 (12 ),5249-51

Lai, C F; Ripperger, J; Morella, K K; Wang, Y; Gearing, D P; Horseman, N D; Campos, S P; Fey, G H; Baumann, H (1995. )STAT3 and STAT5B are targets of two different signal pathways activated by hematopoietin receptors and control transcription via separate cytokine response elements. The Journal of biological chemistry, ,270 (40 ),23254-7

Horseman, N D; Buntin, J D (1995. )Regulation of pigeon cropmilk secretion and parental behaviors by prolactin.Annual review of nutrition, ,15 ,213-38

Horseman, N D; Yu-Lee, L Y (1994. )Transcriptional regulation by the helix bundle peptide hormones: growth hormone, prolactin, and hematopoietic cytokines.Endocrine reviews, ,15 (5 ),627-49

Horseman, N D (1994. )Famine to feast--growth hormone and prolactin signal transducers.Endocrinology, ,135 (4 ),1289-91

Kaetzel, M A; Pula, G; Campos, B; Uhrin, P; Horseman, N; Dedman, J R (1994. )Annexin VI isoforms are differentially expressed in mammalian tissues. Biochimica et biophysica acta, ,1223 (3 ),368-74

Chen, X; Horseman, N D (1994. )Cloning, expression, and mutational analysis of the pigeon prolactin receptor.Endocrinology, ,135 (1 ),269-76

Gao, Y; Horseman, N D (1994. )Structural and functional divergences of the columbid annexin I-encoding cp37 and cp35 genes. Gene, ,143 (2 ),179-86

Sidis, Y; Horseman, N D (1994. )Prolactin induces rapid p95/p70 tyrosine phosphorylation, and protein binding to GAS-like sites in the anx Icp35 and c-fos genes.Endocrinology, ,134 (4 ),1979-85

Sidis, Y; Horseman, N D (1993. )The hinge region of chicken annexin I contains no site for tyrosine phosphorylation. FEBS letters, ,329 (3 ),296-300

Uhrin, P; Horseman, N D (1993. )Regulation of the annexin Icp35 gene in transfected mammary gland cell lines. Biochemistry and molecular biology international, ,30 (2 ),305-10

Haigler, H T; Mangili, J A; Gao, Y; Jones, J; Horseman, N D (1992. )Identification and characterization of columbid annexin Icp37. Insights into the evolution of annexin I phosphorylation sites. The Journal of biological chemistry, ,267 (27 ),19123-9

Horseman, N D; Chen, X; Liu, L; Poyet, P; Hitti, Y (1992. )Cell and species distribution of prolactin-inducible annexin I mRNA. General and comparative endocrinology, ,85 (3 ),405-14

Xu, Y H; Horseman, N D (1992. )Nuclear proteins and prolactin-induced annexin Icp35 gene transcription.Molecular endocrinology (Baltimore, Md.), ,6 (3 ),375-83

Hitti, Y S; Horseman, N D (1991. )Structure of the gene encoding columbid annexin Icp35. Gene, ,103 (2 ),185-92

Horseman, N D (1990. )Hormonal regulation of an avian annexin I gene. Biochemical Society transactions, ,18 (6 ),1113-5

Young, K H; Buhi, W C; Horseman, N; Davis, J; Kraeling, R; Linzer, D; Bazer, F W (1990. )Biological activities of glycosylated and nonglycosylated porcine prolactin. Molecular and cellular endocrinology, ,71 (2 ),155-62

Horseman, N D (1989. )A prolactin-inducible gene product which is a member of the calpactin/lipocortin family.Molecular endocrinology (Baltimore, Md.), ,3 (5 ),773-9

Horseman, N D; Hankovich, S (1989. )A chamber and method which simplify fixing ultrathin sequencing gels. BioTechniques, ,7 (4 ),340, 342, 344

Pauly, J R; Horseman, N D (1988. )Autoradiographic localization of alpha-bungarotoxin binding sites in the suprachiasmatic region of rat brain. Brain research, ,452 (1-2 ),105-12

Pauly, J R; Horseman, N D (1988. )Perturbations of locomotor activity rhythms following suprachiasmatic bungarotoxin infusion. Physiology & behavior, ,43 (6 ),859-65

Pukac, L A; Horseman, N D (1987. )Regulation of cloned prolactin-inducible genes in pigeon crop.Molecular endocrinology (Baltimore, Md.), ,1 (2 ),188-94

Pauly, J R; Horseman, N D (1985. )Anticholinergic agents do not block light-induced circadian phase shifts. Brain research, ,348 (1 ),163-7

Horseman, N D; Nollin, L J (1985. )The mitogenic, but not differentiative, response of crop tissue to prolactin is circadian phase dependent.Endocrinology, ,116 (5 ),2085-9

Pukac, L A; Horseman, N D (1984. )Regulation of pigeon crop gene expression by prolactin.Endocrinology, ,114 (5 ),1718-24

Horseman, N D; Ehret, C F (1982. )Glucocorticosteroid injection is a circadian zeitgeber in the laboratory rat. The American journal of physiology, ,243 (3 ),R373-8

Horseman, N D; Meier, A H (1979. )Circadian phase-dependent prolactin mechanisms in hepatic lipogenesis of a teleost. The Journal of endocrinology, ,82 (3 ),367-72

Horseman, N D; Meier, A H (1979. )Circadian-dependent prolactin effects: hepatic RNA metabolism and prostaglandin mediation. General and comparative endocrinology, ,38 (3 ),269-74

Ottenweller, J E; Meier, A H; Ferrell, B R; Horseman, N D; Proctor, A (1978. )Extrapituitary regulation of the circadian rhythm of plasma corticosteroid concentration in rats.Endocrinology, ,103 (5 ),1875-9

Horseman, N D; Meier, A H (1978. )Prostaglandin the osmoregulatory role of prolactin in a teleost. Life sciences, ,22 (17 ),1485-90

Horseman, N D; Meier, A H; Culley, D D (1976. )Daily variations in the effects of disturbance on growth, fattening, and metamorphosis in the bullfrog (Rana catesbeiana) tadpole.The Journal of experimental zoology, ,198 (3 ),353-8

Honors and Awards

Hall of Distinguished Alumni Award Highest award of the EKU International Alumni Association. This award is presented to an EKU graduate who has served others, achieved professional prominence, and made significant contributions to society. EKU International Alumni Association Status:Recipient Type:Recognition

Student Advising

Sam Kunkle (Master )Student Thesis Preceptor

Aaron Marshall (Doctoral )Chair Status:Completed

Vaibhav Pai (Doctoral )Chair Status:Completed

Laura Hernandez (Postdoctoral )Supervisor

Vaibhav P. (Postdoctoral )Supervisor Status:Completed

Lauren Haar (Doctoral )Lab Rotation Preceptor

Archie Vomachka, Ph.D. (Other )Status:Completed

Service

(ARPT committee )Committee Member Type:Departmental Service Level:Department

(SBP Retreat Planning Committee )Committee Member Type:Departmental Service Level:Department

(Search Committee )Co-Chair Type:Departmental Service Level:Department

(Life Sciences Review Advisory Committee )Committee Chair Type:University/College Service Level:University

(Searle Scholars Review Committee )Committee Member Type:University/College Service Level:College

Keywords

Prolactin,Transfection,Reporter Gene,Genetic Mapping,Protein Sequence,Western Blotting,Genetic Regulation,DNA Binding Protein,Tissue Cell Culture,Transcription Factor,Genetic Transcription,In Situ Hybridization,Nucleic Acid Sequence,Gel Mobility Shift Assay,Genetic Promoter Element,Polymerase Chain Reaction,Site Directed Mutagenesis,Genetic Regulatory Element,Hormone Regulation Control Mechanism,Breast Cancer,Lactation,Apoptosis,Serotonin,TGF-beta

Research and Practice Interests

The hormonal control of breast development and breast cancer risk are poorly understood, despite decades of active research. The mammary gland is biologically unique in that mammary gland organogenesis occurs postnatally and is controlled primarily by systemic endocrine factors, making it accessible to detailed study. Clinically, breast cancers are the second leading cause of cancer deaths in women, and the rate of breast cancer occurrence has risen steadily for at least 6 decades. The main hormones that appear to determine breast development are estrogen and prolactin. 

We knocked out the prolactin gene of mice and discovered that prolactin is required at a stage of development during which precursor cells for the secretory epithelium differentiate from the ducts, which is a critical determinant of breast cancer risk. 

The molecular basis of prolactin action is being investigated using both whole animal and cell culture systems, and in animal and human systems. We have cloned prolactin-regulated genes, the prolactin receptor, and several prolactin-activated transcription factors. One current project is focusing on identifying novel molecular correlates of prolactin-induced mammary development. 

Our current focus is on identifying genes that act during the earliest stages of mammary gland differentiation. We have used differential hybridization to gene microarrays and suppression subtractive hybridization cloning to discover gener that are specifically regulated by prolactin during mammary gland development. Our studies have identified novel pathways involving matrix protein kinases and monoamine biosythetic enzymes that mediate prolactin actions on breast development and breast physiology. 

Professional Affiliation

Invited speaker, Gordon Conference on Mammary Gland Biology

Courses Taught

26-MCP-842 MED PHYSIOLOGY II Level:Graduate

26-MCP-904 HUMAN SYS PHYS III

Patents and Inventions

12/721,296 Control of Milk Production and Mammary Involution NDH, Robert Collier, Aaron Marshall, Laura Hernandez, Filed Co-Inventor

Contact Information

Academic - Department of Molecular and Cellular Physiology
Phone: 513-558-3019
Nelson.Horseman@uc.edu