In-Kwon Kim

In-Kwon Kim

Assistant Professor

Crosley Tower

802

A&S Chemistry - 0172

Professional Summary

In-Kwon Kim is a structural biochemist with research interests in poly(ADP-ribose) metabolism and signaling, DNA damage response, and cancer drug discovery. After completing a B.S. degree from Seoul National University in 1998, he obtained his M.Sc (2000; Microbiology) and Ph.D. (2005; Biochemistry and Structural Biology) degree under the direction of Prof. Sa-Ouk Kang from Seoul National University. In 2006, he joined the research group of Prof. Tom Ellenberger at Washington University School of Medicine as a post-doc and was promoted into (Research) Assistant Professor. Since then, his research has been focused on the mechanistic structural biochemistry and drug discovery of key enzymes in poly(ADP-ribose) metabolism and signaling. He also worked in the Washington University Drug Discovery Program, led by Prof. Michael Holtzman. He joined the faculty at University of Cincinnati in 2016 as part of the university-wide Cancer Cluster Hiring Initiative. His long-term research goal is to understand the mechanism and function of poly(ADP-ribose) turnover and develop new tumor-selective therapeutics. He is collaborating with a number of world-leading scientists in the fields, including Joel Moss (Deputy Chief in NHLBI/NIH). For details, please visit his laboratory homepage (http://homepages.uc.edu/~kimiw/).

Education

Ph.D., Seoul National University (Korea), 2005 (Biochemistry and Structural Biology)

M.S., Seoul National University (Korea), 2000 (Microbiology)

B.S., Seoul National University (Korea), 1998 (Microbiology)

Positions and Work Experience

2016 -To Present Assistant Professor of Chemistry, Cancer Cluster Hiring Initiative of UC, University of Cincinnati, Cincinnati, OH

2015 -2016 Instructor in Medicine and Drug Discovery Program, Washington University School of Medicine, St. Louis, MO

2012 -2015 (Research) Assistant Professor of Biochemistry & Molecular Biophysics, Washington University School of Medicine, St. Louis, MO

Research and Practice Interests

  • Mechanism and pharmacological control of poly(ADP-ribose) turnover and signaling
  • Nucleic acid enzymology in DNA damage response
  • Development of new tumor-selective therapeutics

Research Support

Grant: #133405-RSG-19-200-01-DMC Investigators:Kim, In-Kwon 01-01-2020 -12-31-2023 American Cancer Society - Research Scholar Grant (RSG) Mechanism and Control of Poly(ADP-ribose) Turnover Role:PI $792,000.00 Active Type:Grant Level:National

Grant: #V2018-025 Investigators:Kim, In-Kwon 11-01-2018 -10-31-2020 The V Foundation 2018 V Scholar Grant - Mechanism and Pharmacological Control of Human ADP-ribosyl-acceptor Hydrolase 3 Role:PI $200,000.00 Active Type:Grant Level:National

Grant: #N/A Investigators:In-Kwon Kim 09-01-2018 -02-28-2021 University of Cincinnati Cancer Institute 2018 Marlene Harris Ride Cincinnati Pilot Grant Award - Mechanism and Pharmacological Control of Human ADP-ribosyl-acceptor Hydrolase 3 Role:PI $35,000.00 Active Type:Grant Level:Local

Grant: #UH2 HL123429 Investigators:Holtzman, Michael; Kim, In-Kwon et al. 09-22-2014 -06-30-2016 NIH/NHLBI "Preclinical Development of an Anti-Mucus Drug" Role:Co-I $973,826/yr Completed Type:Grant Level:National

Grant: #R01 AI111605 Investigators:Holtzman, Michael; Kim, In-Kwon et al. 08-01-2014 -07-31-2016 NIH/NIAID "Interferon Signal Enhancers as Antiviral Therapeutics" Role:Co-I $289,137/yr Completed Type:Grant Level:National

Grant: #U19 AI070489 Investigators:Holtzman, Michael; Kim, In-Kwon et al. 08-01-2011 -07-31-2016 NIH/NIAID “Epithelial Barrier Programs in Asthma and Allergic Disease” Project 3: "Project 3 Epithelial Injury and TLR3 Role:Co-I $159,333/yr Completed Type:Grant Level:National

Grant: #R01GM141226 Investigators:Kim, In-Kwon 05-01-2021 -02-28-2025 National Institute of General Medical Sciences Reversal of residue-specific ADP-ribosylations by ADP-ribosyl-acceptor hydrolases Role:PI $334,910.00 Awarded Level:Federal

Abbreviated Publications

Peer Reviewed Publications

Houl, J.H., Ye, Z., Brosey, C.A., Balapiti-Modarage, L.P.F., Namjoshi, S., Bacolla, A., Laverty, D., Walker, B.L., Pourfarjam, Y., Warden, L.S., Chinnam, N.B., Moiani, D., Stegeman, R.A., Chen, M.K., Hung, M.C., Nagel, Z.D., Ellenberger, T., Kim, I.K.#, Jones, D.E., Ahmed, Z.# and Tainer, J.A.# (2019) Selective small molecule PARG inhibitor causes replication fork stalling and cancer cell death. Nature Communications, in press. (#: Co-corresponding author)

Pourfarjam, Y., Ventura, J., Kurinov, I., Cho, A., Moss, J., and Kim, I.K. (2018). Structure of human ADP-ribosyl-acceptor hydrolase 3 bound to ADP-ribose reveals a conformational switch that enables specific substrate recognition. Journal of Biological Chemistry. 293, 12350-12359, PMCID: PMC6093245

Kim, I.K.**, Stegeman, R.A., Brosey, C.A., & Ellenberger, T.** (2015). A quantitative assay reveals ligand specificity of the DNA scaffold repair protein XRCC1 and efficient disassembly of complexes of XRCC1 and the poly(ADP-ribose) polymerase 1 by poly(ADP-ribose) glycohydrolase. J. Biol. Chem., 290, 3775-3783 (** Co-corresponding author).

Kukshal, V., Kim, I.K., Hura, G.L., Tomkinson, A., Tainer, J.A., & Ellenberger, T. (2015). Human DNA ligase III bridges two DNA ends to promote specific intermolecular end joining. Nucleic Acid Res., 43, 7021-7031.

Cheruiyot, A*, Paudyal, S.C.*, Kim, I.K.*, Sparks, M., Ellenberger, T., Helen Piwnica-Worms, & You, Z. (2015). Poly(ADP-ribose)-binding promotes Exo1 damage recruitment and suppresses its nuclease activities. DNA repair (Amst), 35, 106-115 (* Co-first author).

Chen, X, Kim, I.K., Honaker, Y. Paudyal, S.C., Koh, W.K., Sparks, M., Li, S., Piwnica-Worms, H., Ellenberger, T., & You, Z. (2015). 14-3-3 Proteins restrain the Exo1 nuclease to prevent over-resection. J. Biol. Chem., 290, 12300-12312.

Kim, I.K., Kiefer, J.R., Ho, C.M.W., Stegeman, R.A., Classen, S., Tainer, J.A., & Ellenberger, T. (2012). Structure of mammalian poly(ADP-ribose) glycohydrolase reveals a flexible tyrosine clasp as a substrate-binding element. Nat. Struct. Mol. Biol., 19, 653-656.

Cotner-Gohara, E.*, Kim, I.K.*, Hammel, M., Tainer, J.A., Tomkinson, A.E., & Ellenberger, T. (2010). Human DNA ligase III recognizes DNA ends by dynamic switching between two DNA-bound states. Biochemistry, 49, 6165-6176 (* Co-first author).

Hammel, M., Rashid, I., Sverzhinsky, A., Pourfarjam, Y., Tsai, M.S., Ellenberger, T., Pascal, J.M., Kim, I.K.¶, Tainer, J.A., and Tomkinson, A.E. An atypical BRCT-BRCT interaction with human DNA Ligase IIIa compacts the XRCC1 scaffold protein within a flexible DNA repair complex. in press. Nucleic Acid Res. (¶: Co-corresponding author)

Pourfarjam, Y., Kasson, S., Tran, L., Ho, C. and Kim, I.K. (2020) PARG has a robust endo-glycohydrolase activity that releases protein-free poly(ADP-ribose) chains. Biochemical and Biophysical Research Communications. 527(3), 818-823, PMID: 32439163.

Presentations

Invited Presentations

Kim, In-Kwon (04-2018. ) Conformational changes of human ARH3 enable specific recognition and cleavage .The Cold Spring Harbor Laboratory Meeting; The PARP Family and ADP-ribosylation, Cold Spring Harbor, NY, USA. Conference. . Level:International

Kim, In-Kwon (04-2014. ) Mechanism and function of poly(ADP-ribose) turnover by mammalian poly(ADP-ribose) glycohydrolase .Cold Spring Harbor Laboratory Meeting: The PARP Family & Friends: Gene Regulation and Beyond, Cold Spring Harbor, NY.

Kim, In-Kwon (03-2014. ) Mechanism and function of poly(ADP-ribose) turnover by poly(ADP-ribose) glycohydrolase .13th Structural Cell Biology of DNA Repair Machines (SBDR) Workshop, Berkeley, CA.

Kim, In-Kwon (04-2013. ) Structure and function of mammalian poly(ADP-ribose) glycohydrolase .12th Structural Cell Biology of DNA Repair Machines (SBDR) Workshop, Berkeley, CA.

In-Kwon Kim (05-2019. ) Reversal of residue-specific ADP-ribosylations by ADP-ribosyl-acceptor hydrolases .NSF CAREER workshop, Alexandria, VA. Level:National

In-Kwon Kim (02-21-2020. ) University of Cincinnati Chemistry Ph.D. program and Biochemistry Research .Estern Kentucky University, KY. Other Institution. . Level:Regional

Poster Presentations

Yasin Pourfarjam and In-Kwon Kim (05-2017. ) Mechanism and regulation of human Poly(ADP-ribose) Glycohydrolase (PARG) .CBSB conference, UC. . Level:International

Yasin Pourfarjam, Ah-Ra Cho, and In-Kwon Kim (11-2017. ) Discovery and Characterization of Proteins Modulating Poly(ADP-ribose) Glycohydrolase (PARG) .Oesper symposium, UC. . Level:National

Yasin Pourfarjam and In-Kwon Kim (11-2018. ) Structure and Catalytic Mechanism of ADP-ribosyl-acceptor hydrolase 3 (ARH3) .Oesper symposium, UC. . Level:National

Lecture

In-Kwon Kim (12-2016. ) Mechanism and pharmacological control of poly(ADP-ribose) turnover and signaling .University of Cincinnati medical Center. UC. Level:Department

Kim, In-Kwon (10-2016. ) Mechanism and pharmacological control of poly(ADP-ribose) turnover and signaling .University of Cincinnati Medical Center. UC. Level:Department

Kim, In-Kwon (05-2013. ) Structure and function of mammalian poly(ADP-ribose) glycohydrolase .Korea. Other Institution.

Event Organized

GRW 2018

GRW 2018

Honors and Awards

11-2018 -10-2020 V scholar young investigator award, V foundation, $200,000 Status:Recipient Level:National Type:Grant

01-2018 -07-2018 Faculty Development Fund Travel to professional conference $1,918 Status:Recipient Level:University Type:Fellowship

09-2018 -02-2020 Marlene Harris Ride Cincinnati Pilot Grant Award Status:Recipient Level:Local Type:Grant

Service

Department of Chemistry (Graduate Admission Committee ) Committee Member Type:Departmental Service Level:University

Department of Chemistry (Moral Committee ) Committee Member Type:Departmental Service Level:University

Department of Chemistry Member Type:Departmental Service Level:Department

Department of Chemistry Program Operator Type:Departmental Service Level:Department

Department of Chemistry (Graduate Admission Committee ) Committee Member Type:Departmental Service Level:Department

Department of Chemistry (Moral Committee ) Committee Member Type:Departmental Service Level:Department

Department of Chemistry (Faculty Search Committee ) Committee Member Type:Departmental Service Level:Department

University of Cincinnati (Bioseparation faculty search committee ) Committee Member Type:Departmental Service Level:Department

UNIVERSITY OF CINCINNATI (Bioanalytical faculty search committee ) Committee Member Type:Departmental Service Level:Department

Post Graduate Training and Education

2011-2012 Staff Scientist, Washington University School of Medicine, , St. Louis, MO

2006-2011 Postdoctoral Fellow, Washington University School of Medicine, , St. Louis, MO

Professional Affiliation

Member Korean-American Scientists and Engineers Association (KSEA),

Member Korean-American Scientists and Engineers Association (KSEA),

Courses Taught

Advanced Biochemistry IV Level:Graduate

Survey of Biochemistry Laboratory I Level:Undergraduate

-CHEM-3040 INTRO BIOCHEM Level:Undergraduate

-CHEM-3040 INTRO BIOCHEM Level:Undergraduate

-CHEM-2031L SURVEY BIOCH LAB II Level:Undergraduate

-CHEM-3040 INTRO BIOCHEM Level:Undergraduate

-CHEM-4040 MOLECULES OF LIFE Level:Undergraduate