Patrick Limbach

Patrick A Limbach , Ph.D.

VP for Research

Professor and Ohio Eminent Scholar

Cincinnati, Ohio Phone 513-558-0026
Email Pat.Limbach@uc.edu

Professional Summary

Pat Limbach is a Professor and Ohio Eminent Scholar at the University of Cincinnati. He is a bioanalytical chemist with research interests in mass spectrometry, modified ribonucleic acids (RNAs), ribonucleoprotein (RNP) complexes and protein translation. After earning an undergraduate degree from Centre College in 1988, he studied under the direction of Dr. Alan G. Marshall at The Ohio State University. While there, his graduate research focused on instrumentation improvements to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. He received his PhD from OSU in 1992. He then took a postdoctoral position at the University of Utah working with Dr. James A. McCloskey. While in Utah, he worked in the areas of RNA chemistry and nucleic acid mass spectrometry. In 1995, he joined the faculty at Louisiana State University as an Assistant Professor and was promoted to Associate Professor in 1999. In 2001, he moved to his current position in Cincinnati, being promoted to Professor in 2005. He has served as Department Head in Chemistry at UC from 2005-2010 and as Interim Associate Dean for Research and Graduate Studies in the College of Arts and Science of Arts and Sciences in 2013.  Dr. Limbach and his group seek to advance the area of mass spectrometry within modified RNAs and RNPs and collaborate extensively with researchers inside the state of Ohio and throughout the world. Dr. Limbach is an active member of the American Chemical Society, American Society for Mass Spectrometry, Sigma Xi and a lifetime member of Phi Kappa Phi. He also serves on the Scientific Advisory Board for RiboNova, Inc.
You can learn more about Dr. Limbach's research program at his group's website - http://bearcatms.uc.edu

Education

PhD: Ohio State University 1992

B.S.: Centre College Danville, KY, 1988 (Chemistry and Chemical Physics)

Research and Practice Interests

mass spectrometry
modified nucleosides
transfer RNAs
structural investigation of nucleic acids
characterization of ribonucleoprotein complexes
assays for therapeutic oligonucleotides
nucleic acid biomarkers in humans

Positions and Work Experience

1995 -1999 Assistant Professor Chemistry, Louisiana State University, Baton Rouge, LA

1999 -2001 Associate Professor Chemistry, Louisiana State University, Baton Rouge, LA

2001 -2003 Associate Professor Chemistry, University of Cincinnati, Cincinnati, OH

2003 -To Present Professor Chemistry, University of Cincinnati, Cincinnati, OH

2005 -2010 Department Head, Chemistry,

2013 - Interim Associate Dean Research and Graduate Affairs, College of Arts and Science of Arts & Sciences,

Research Support

Grant: #R01-GM58843 Investigators:Limbach, Patrick 02-01-1999 -01-31-2004 National Institute of General Medical Sciences Identification of Modified Nucleosides in Ribosomal Ribonucleic Acid Role:PI Completed Type:Grant

Grant: #2001-HIF03 Investigators:Limbach, Patrick 06-20-2002 -06-30-2004 Ohio Board of Regents Mass Spectrometry Consortium for Materials and Medical Research Role:co-PI Completed Type:Grant

Grant: #SRS 00566 Investigators:Limbach, Patrick 01-01-2007 -06-30-2008 Pfizer, Inc. Quantification of Macromolecules Role:PI $127,485.00 Active Level:Industry

Grant: #1S10RR019900-01 Investigators:Limbach, Patrick 04-25-2005 -04-24-2006 National Center for Research Resources Acquisition of a Hybrid Fourier Transform Mass Spectrometer for Proteomics Role:PI $917,973.00 Closed Level:Federal

Grant: #3-R01-GM58843-04 NCE Investigators:Limbach, Patrick 02-01-2002 -01-31-2004 National Institute of General Medical Sciences Identification of Modified Nucleosides in Ribosomal Ribonucleic Acid Role:PI $150,557.00 Closed Level:Federal

Grant: #CHE-0910751 Investigators:Limbach, Patrick 08-01-2009 -07-31-2012 National Science Foundation Sequencing Transfer RNAs by Mass Spectrometry Role:PI $450,000 Active Type:Grant

Grant: #2001-HIF03 Investigators:Limbach, Patrick 06-20-2002 -06-30-2004 Ohio Board of Regents Mass Spectrometry Consortium for Materials and Medical Research Role:PI $179,900.00 Closed Level:State of Ohio

Grant: #R01GM069547 Investigators:Limbach, Patrick 01-01-2004 -12-31-2008 National Institute of General Medical Sciences Bioassays and Molecular Characterization of Microchip CE Role:PI $1,436,594.00 Closed Level:Federal

Grant: #SRS 005253 Investigators:Limbach, Patrick 09-18-2007 -03-18-2009 Girindus America Inc. The Development of Analytical Methods for Quantitation of Oligonucleotides Role:PI $140,000.00 Active Level:Industry

Grant: #S10 RR027671 Investigators:Limbach, Patrick 04-15-2010 -04-14-2011 National Center for Research Resources Acquisition of an LC Mass Spectrometer for Nucleic Acids Research Role:PI $500,000 Completed Type:Grant

Grant: #SRS 006051 Investigators:Limbach, Patrick 06-26-2008 -12-31-2008 Chevron U.S.A Chemistry Facilities Services Role:PI $12,000.00 Closed Level:Industry

Grant: #CHE-0910751 Investigators:Limbach, Patrick 08-01-2009 -07-31-2012 National Science Foundation Sequencing Transfer RNAs by Mass Spectrometry Role:PI $450,000.00 Closed Level:Federal

Grant: #1 S10 RR027671-01 Investigators:Limbach, Patrick 04-15-2010 -04-14-2011 National Center for Research Resources Acquisition of an LC Mass Spectrometer for Nucleic Acids Research Role:PI $500,000.00 Completed

Grant: #R01 GM058843 Investigators:Limbach, Patrick 09-30-2010 -06-30-2014 National Institute of General Medical Sciences Mass Spectrometry of Ribosomal RNA: Protein Interactions Role:PI $1,047,547 Completed Type:Grant

Grant: #HDTRA 1-15-1-0033 Investigators:Limbach, Patrick 02-17-2015 -02-16-2016 Defense Threat Reduction Agency Investigating Radiation-Induced Damage During Translation in Melanized Fungi Role:PI $147,333 Active Type:Grant

Grant: #SRS 00566 Investigators:Limbach, Patrick 01-01-2007 -06-30-2008 Pfizer, Inc. Quantification of Macromolecules Role:PI Completed Type:Grant

Grant: #S10 RR019900 Investigators:Limbach, Patrick 04-25-2005 -04-24-2006 National Center for Research Resources Acquisition of a Hybrid Fourier Transform Mass Spectrometer for Proteomics Role:PI Completed Type:Grant

Grant: #S10 OD018485 Investigators:Limbach, Patrick 04-01-2015 -03-31-2016 Office of the Director, National Institutes of Health Acquisition of a High Resolution Mass Spectrometer for the University of Cincinnati Role:PI $963,500 Active Type:Grant

Grant: #CHE-1212625 Investigators:Limbach, Patrick 08-15-2012 -07-31-2015 National Science Foundation Comparative Analysis of Transfer RNAs by Mass Spectrometry Role:PI $496,967.00 Closed Level:Federal

Grant: #CHE-1507357 Investigators:Limbach, Patrick 07-01-2015 -06-30-2018 National Science Foundation Multiplexed Mass Spectrometry of Modified RNAs Role:PI $498,476 Active Type:Grant

Grant: #UC Davis sub R01GM113888 Investigators:Addepalli, Balasubrahmanyam; Limbach, Patrick 03-01-2016 -02-28-2020 National Institute of General Medical Sciences Recombinant microRNAs in xenobiotic disposition Role:PI $132,697.00 Active Level:Federal

Grant: #R01 GM069547 Investigators:Limbach, Patrick 01-01-2004 -12-31-2008 National Institute of General Medical Sciences Bioassays and Molecular Characterization of Microchip CE Role:PI Completed Type:Grant

Grant: #SRS 005253 Investigators:Limbach, Patrick 09-18-2007 -03-18-2009 Girindus America Inc. The Development of Analytical Methods for Quantitation of Oligonucleotides Role:PI $90,000 Completed Type:Contract

Grant: #R01 GM058843 Investigators:Limbach, Patrick 08-01-2015 -07-31-2019 National Institute of General Medical Sciences Mass Spectrometry of Modified RNAs Role:PI $1,054,466 Active Type:Grant

Grant: #RUC002 / R42DA042467 Investigators:Addepalli, Balasubrahmanyam; Limbach, Patrick 09-01-2016 -02-28-2017 National Institute on Drug Abuse PHASE 1 Development of novel research tools and a database for mapping human mitochondrial tRNA modifications by mass spectrometry Role:PI $49,723.00 Active Level:Federal

Grant: #R41 DA038970 Investigators:Limbach, Patrick 02-01-2015 -01-31-2016 National Institutes of Health Novel mass spectrometry methods for profiling modified RNA nucleotides in the nervous system Role:PI $74,726 Active Type:Grant

Grant: #RUC 003 / R44DA042467 Investigators:Addepalli, Balasubrahmanyam; Limbach, Patrick 03-01-2017 -02-28-2018 National Institute on Drug Abuse PHASE 2 Development of novel research tools and a database for mapping human mitochondrial tRNA modifications by mass spectrometry Role:PI $282,588.00 Active Level:Federal

Grant: #CHE-1828072 Investigators:Ayres, Neil; Connick, William; Limbach, Patrick; Mack, James; Merino, Edward 08-15-2018 -07-31-2021 National Science Foundation MRI: Acquisition of a TOF Mass Spectrometer for Research and Education Role:Collaborator $339,500.00 Active Level:Federal

Grant: #Action Fund 1355 (see 011727) Investigators:Ayres, Neil; Connick, William; Limbach, Patrick; Mack, James; Merino, Edward 08-15-2018 -07-31-2021 Ohio Department of Higher Education MRI: Acquisition of a TOF Mass Spectrometer for Research and Education Role:Collaborator $72,750.00 Active Level:State of Ohio

Grant: #EPA/ORD Master MOU Investigators:Limbach, Patrick 04-01-2019 -03-31-2024 Environmental Protection Agency MOU between the EPA and UC Role:PI $.00 Active Level:Federal

Grant: #CHE-0602413 Investigators:Limbach, Patrick 07-01-2006 -06-30-2010 National Science Foundation New Methods for the Analysis of Ribonucleic Acids Role:PI $436,988 Completed Type:Grant

Grant: #RUC005 (Collections Agreement for Awd# 010841) Investigators:Addepalli, Balasubrahmanyam; Limbach, Patrick 01-02-2020 -02-15-2020 RiboNova Inc. Development of novel research tools and a database for mapping human mitochondiral tRNA modificaations by mass spectrometry Role:PI $45,534.38 Awarded Level:Industry

Grant: #UTA20-000910 / R21ES032124 Investigators:Limbach, Patrick 08-11-2020 -07-31-2022 National Institute of Environmental Health Sciences Induced Regulation of m6A methylations via ADAR oxidation Role:PI $31,177.00 Awarded Level:Federal

Grant: #R01 GM058843 Investigators:Limbach, Patrick 12-01-2004 -08-31-2009 National Institute of General Medical Sciences Mass Spectrometry of Ribosomal RNA: Protein Interactions Role:PI $1,050,772 Completed Type:Grant

Investigators:Addepalli, Balasubrahmanyam; Limbach, Patrick 04-01-2021 -03-31-2024 National Cancer Institute Recombinant microRNAs in xenobiotic and nutrient disposition Role:PI 63761.00 Awarded Level:Federal

Grant: #DBI-0137924 Investigators:Limbach, Patrick 04-01-2002 -03-31-2005 National Science Foundation Single Cell Proteomics Role:PI Completed Type:Grant

Grant: #R01GM058843 Investigators:Addepalli, Balasubrahmanyam; Limbach, Patrick 02-01-2022 -12-31-2025 National Institute of General Medical Sciences Mass Spectrometry of Modified RNAs Role:PI 371560.00 Awarded Level:Federal

Grant: #R01 GM058843-08S1 Investigators:Limbach, Patrick 07-17-2009 -11-30-2009 National Institute of General Medical Sciences Mass Spectrometry of Ribosomal RNA: Protein Interactions Role:PI $102,341 Completed Type:Grant

Grant: #CHE-1212625 Investigators:Limbach, Patrick 08-15-2012 -07-31-2015 National Science Foundation Comparative Analysis of Transfer RNAs by Mass Spectrometry Role:PI $496,967 Completed Type:Grant

Grant: #R56 GM058843 Investigators:Limbach, Patrick 09-01-2009 -08-31-2010 National Institute of General Medical Sciences Mass Spectrometry of Ribosomal RNA: Protein Interactions Role:PI $330,000 Completed Type:Grant

Grant: #CHE-0962996 Investigators:Limbach, Patrick 09-15-2010 -08-31-2013 National Science Foundation Renovation of Chemistry Facilities at the University of Cincinnati Role:PI $1,200,000 Completed Type:Grant

Abbreviated Publications

Peer Reviewed Publications

Mandal, D., Köhrer, C., Su, D., Russell, S.P., Krivos, K., Castleberry, C.M., Blum, P., Limbach, P.A., Söll, D., RajBhandary, U.L. “Agmatidine, a modified cytidine in the anticodon of archaeal tRNAIle, base pairs with Adenosine but not with Guanosine”, Proceedings of the National Academy of Sciences, USA 107 (2010) 2872-2877. PMCID: PMC2840323.

Krivos, K., Limbach, P.A. “Sequence Analysis of Peptide:Oligonucleotide Heteroconjugates by Electron Capture Dissociation and Electron Transfer Dissociation”, Journal of the American Society for Mass Spectrometry 21 (2010) 1387-97. PMID: 204335485.

Anton, B.P.; Russell, S.P.; Limbach, P.A.; Kasif, S.; Raleigh, E.A.; Roberts, R.J. “Functional Characterization of the YmcB and YqeV tRNA Methylthiotransferases of Bacillus subtilis”, Nucleic Acids Research 38 (2010) 6195-205. PMCID: PMC2952846

Nikcevic, I.; Wyrzykiewicz, T.K.; Limbach, P.A.; “Detecting Low-level Synthesis Impurities in Modified Phosphorothioate Oligonucleotides Using Liquid Chromatography-High Resolution Mass Spectrometry”, International Journal of Mass Spectrometry 304 (2011), 98-104.More Information

Castleberry, C. M.; Limbach, P.A.; “Relative Quantitation of Transfer RNAs Using Liquid Chromatography-Mass Spectrometry (LC-MS) and Signature Digestion Products”, Nucleic Acids Research 38 (2010) e162. PMCID: PMC2938229

Nikcevic, I.; Piruska, A.; Wehmeyer, K.R.; Seliskar, C.J.; Limbach, P.A.; Heineman, W.R., “Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser induced fluorescence detection”, Electrophoresis 31 (2010) 2796-803. PMCID: PMC3031587.

Easter, R.N.; Kröning, K.K; Caruso, J. A.; Limbach, P.A.; “Separation and detection of oligonucleotides by hydrophilic interaction liquid chromatography (HILIC) - inductively coupled plasma mass spectrometry (ICPMS)”, Analyst 135 (2010) 2560-2565. PMID: 20830328

Addepalli, B.; Limbach, P.A.; Hunt, A.G “Disulfide Linkage in a CCCH Zinc Finger Motif of Arabidopsis CPSF30 Ortholog”, FEBS Letters 584 (2010) 4408-4412. PMID: 20888817

Currie, C.A.; Shim, J.S.; Lee, S.H.; Ahn, C.; Limbach, P.A.; Halsall, H.B.; Heineman, W.R. “Comparing Polyelectrolyte Multilayer-coated PMMA Microfluidic Devices and Glass Microchips for Electrophoretic Separations”, Electrophoresis 30 (2009) 4245-4250. PMCID: PMC2847454.

Castleberry, C.M.; Lilleness, K.; Baldauff, R.; Limbach, P.A. “Minimizing 18O/16O Back-exchange in the Relative Quantification of RNAs”, Journal of Mass Spectrometry 44 (2009) 1195-1202. PMID: 19484804.

Hossain, M.; Limbach, P.A. “Multiple Endonucleases Improve MALDI-MS Signature Digestion Product Detection of Bacterial Transfer RNAs”, Analytical and Bioanalytical Chemistry 394 (2009) 1125-1135. PMID: 19104781

Hamburg, D.-M.; Suh, M.-J.; Limbach, P.A. “Limited Proteolysis of the Ribosome is Affected by Subunit Assembly”, Biopolymers 91 (2009) 410-422. PMCID: PMC2936250.

Hossain, M.; Limbach, P.A.; “Mass Spectrometry-Based Detection of Transfer RNAs by Their Signature Endonuclease Digestion Products” RNA 13 (2007) 295-303. PMCID: PMC1781365.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "Frontal analysis in microchip capillary electrophoresis: a simple and accurate method for determination of protein-DNA dissociation constant". Electrophoresis (2007), 28, 837-842.

Gong, M.; Wehmeyer, K.R.; Stalcup, A.M.; Limbach, P.A.; Heineman, W.R. "Study of injection bias in a simple hydrodynamic injection in microchip capillary electrophoresis". Electrophoresis (2007), 28, 1564-1571.

Nikcevic, I.; Lee, S.-H.; Piruska, A.; Ahn, C.; Ridgway, T.H.; Limbach, P.A.; Wehymeyer, K.R.; Heineman, W.R.; Seliskar, C.J. "Characterization and performance of injection molded poly(methylmethacrylate) microchips for capillary electrophoresis". J. Chrom. A (2007), 1154, 444-453.

Suh, M.J.; Pourshahian, S.; Limbach, P.A. "Developing Limited Proteolysis and Mass Spectrometry for the Characterization of Ribosome Topography". J. Am. Soc. Mass Spectrom. (2007), 18, 1304-1317.

Duderstadt, R.E.; Tsuie, B.M.; Macha, S.F.; Limbach, P.A. "Investigation of Single-Site Zirconium Azaborolinyl Complexes by Laser Desorption Ionization Time-of-Flight Mass Spectrometry". Anal. Chim. Acta (2007), 596, 124-131.

Mecomber, J.S.; Stalcup, A.M.; Hurd, D; Halsall, H.B.; Heineman, W.R.; Seliskar, C.J.; Wehmeyer, K.R.; Limbach, P.A. "Analytical Performance of Polymer-Based Microfluidic Devices Fabricated by CNC Machining". Anal. Chem. (2006), 78, 936-941.

Carr, J.F.; Hamburg, D.-M.; Gregory, S.T.; Limbach, P.A.; Dahlberg, A.E. "Effect of Streptomycin-resistance Mutations on the Post-translational Modification of Ribosomal Protein S12". J. Bacteriol. (2006), 188, 2020-2023.

Meng, Z.; Limbach, P.A. "Mass Spectrometry of RNA: Linking the Genome to the Proteome". Briefings in Functional Genomics & Proteomics (2006), 5, 87-95.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Arias, F.; Heineman, W.R. "On-line Sample Preconcentration using Field-Amplified Stacking Injection in Microchip Capillary Electrophoresis". Anal. Chem. (2006), 78, 3730-3737.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "On-line Sample Preconcentration by Sweeping with Dodecyltrimethylammonium Bromide in Capillary Zone Electrophoresis". J. Chrom. A (2006), 1125, 263-269.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "Unlimited-volume Electrokinetic Stacking Injection in Sweeping Capillary Electrophoresis Using a Cationic Surfactant". Anal. Chem. (2006), 78, 6035-6042.

McLachlan, A.; Borchers, M.; Velayutham, P.; Wagner, M.; Limbach, P.A. "Characterizing the Reproducibility of a Protein Profiling Method for the Analysis of Mouse Bronchoalveolar Lavage Fluid". J. Proteome Res. (2006), 5, 3059-3065.

Wetherill, Y.B.; Hess-Wilson, J.K.; Comstock, C.E.S.; Shah, S.A.; Buncher, C.R.; Sallans, L.; Limbach, P.A.; Schwemberger, S.; Babcock, G.F.; Knudsen, K.E. "Bisphenol A facilitates bypass of androgen ablation therapy in prostate cancer". Mol Cancer Therapeutics (2006), 5, 3181-3190.

Tummala, R.; Limbach, P.A. "Effect of Sodium Dodecyl Sulfate Micelles on Peptide Mass Fingerprinting by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry". Rapid Commun. Mass Spectrom. (2005), 18, 2031-2035.

Meng, Z.; Limbach, P.A. "Quantitation of Ribonucleic Acids Using 18O Labeling and Mass Spectrometry". Anal. Chem. (2005), 77, 1891-1895.

Tu, J.; Halsall, H.B.; Seliskar, C.J.; Limbach, P.A.; Arias, F.; Wehmeyer, K.R.; Heineman, W.R. "Estimation of log Pow Values for Neutral and Basic Compounds by Microchip Microemulsion Electrokinetic Chromatography with Indirect Fluorimetric Detection". J. Pharm. Biomed. Anal. (2005), 38, 1-7.

Mecomber, J.S.; Hurd, D.; Limbach, P.A. "Enhanced machining of micron-scale features in microchip molding masters by CNC milling". Int. J. Machine Tools Manufacture (2005), 45, 1542-1550.

Tummala, R.; Green-Church, K.B.; Limbach, P.A. "Interactions Between Sodium Dodecyl Sulfate Micelles and Peptides During Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) of Proteolytic Digests". J. Am. Soc. Mass Spectrom. (2005), 16, 1438-1446.

Meng, Z.; Limbach, P.A. "Shotgun Sequencing Small Oligonucleotides by Use of Nozzle-Skimmer Dissociation and Electrospray Ionization Mass Spectrometry". Eur. J. Mass Spectrom. (2005), 11, 221-229.

Meng, Z.; Limbach, P.A. "Shotgun Sequencing Small Oligonucleotides by Use of Nozzle-Skimmer Dissociation and Electrospray Ionization Mass Spectrometry". Eur. J. Mass Spectrom., 11, 221-229.

Currie, C.C.; Heineman, W.R.; Halsall, H.B.; Seliskar, C.J.; Limbach, P.A.; Arias, F.; Wehmeyer, K.R. "Estimation of pKa Values Using Microchip CE Coupled with Indirect Fluorescence Detection". J. Chrom. B (2005), 824, 201-205.

Tummala, R.; Limbach, P.A. "Serum Protein Profiling Using Surfactant-Aided Matrix Assisted Laser Desorption/Ionization Mass Spectrometry". Anal. Chim. Acta (2005), 551, 137-141.

Meades, G.D. Jr.; McLachlan, A.; Sallans, L.; Limbach, P.A.; Frankel, L.K.; Bricker, T.M. "Association of the 17 kDa Protein with Photosystem II in Higher Plants". Biochemistry (2005), 44, 15216-15221.

Piruska, A.; Nikcevic, I.; Lee, S.-H.; Ahn, C.; Heineman, W.R.; Limbach, P.A.; Seliskar, C.J. "The Autofluorescence of Plastic Materials and Chips Under Laser Irradiation". Lab Chip (2005), 5, 1348-1354.

Suh, M.-J.; Hamburg, D.-M.; Gregory, S.T.; Dahlberg, A.E.; Limbach, P.A. "Extending Ribosomal Protein Identifications to Unsequenced Bacterial Strains Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry". Proteomics (2005), 5, 4818-4831.

Meng, Z.; Simmons-Willis, T.A.; Limbach, P.A. "The Use of Mass Spectrometry in Genomics". Biomolecular Engineering (2004), 21, 1-13.

Suh, M.-J.; Limbach, P.A. "Investigation of Protein Isolation Methods Suitable for the Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Analysis of Ribonucleoprotein Complexes". Eur. J. Mass Spectrom (2004), 10, 89-99.

Meng, Z.; Limbach, P.A. "RNase Mapping of Intact Nucleic Acids by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (ESI-FTICRMS) and 18O Labeling". Int. J. Mass Spectrom (2004), 234, 37-44.

Cameron, D.M.; Gregory, S.T.; Thompson, J.; Suh, M.-J.; Limbach, P.A.; Dahlberg, A.E. "Thermus thermophilus L11 Methyltransferase, PrmA, is Dispensable for Growth and Preferentially Modifies Free Ribosomal Protein L11 Prior to Ribosome Assembly". J. Bacteriol (2004), 186, 5819-5825.

Berhane, B.T.; Limbach, P.A. "Functional Microfabricated Sample Targets for Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Analysis of Ribonucleic Acids". Anal. Chem. (2003), 75, 1997-2003.

Dominick, W.D.; Berhane, B.T.; Mecomber, J.S.; Limbach, P.A. "Covalent Immobilization of Proteases and Nucleases to Poly(methylmethacrylate)". Anal. Bioanal. Chem (2003), 376, 349-354.

Berhane, B.T.; Limbach, P.A. "Stable Isotope Labeling for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Post-Source Decay Analysis of Ribonucleic Acids". J. Mass Spectrom (2003), 38, 872-878.

Robins, C.R.; Limbach, P.A. "The Use of Nonpolar Matrices for Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Analysis of High Boiling Crude Oil Fractions". Rapid Commun. Mass Spectrom (2003), 17, 2839-2845.

Hassett, D.J.; Limbach, P.A.; Hennigan, R.F.; Klose, K.E.; Hancock, R.E.W.; Platt, M.D.; Hunt, D.F. "Bacterial Biofilms of Importance to Medicine and Bioterrorism: Proteomic Techniques to Identify Novel Vaccine Components and Drug Targets". Exp. Opin. Biol. Therapy (2003), 3, 1201-1207.

Robins, C.L.; Macha, S.F.; Vandell, V.E.; Limbach, P.A. "LDI- and MALDI-Fourier Transform Ion Cyclotron Resonance (FTICR) Mass Spectrometry Analysis of Hydrocarbon Samples". In Analytical Advances in Hydrocarbon Research; Hsu, S., Ed(s); New York, N.Y.: Kluwer Academic/Plenum Publishers, (2003), pp 405-420.

Durairaj, A.; Limbach, P.A. “MALDI-MS Screening for Pseudouridine in Mixtures of Small RNAs by Chemical Derivatization, RNase Digestion and Signature Products” Rapid Communications in Mass Spectrometry 22 (2008) 3727-3734. PMCID: PMC3009585.

Brock, J.E.; Pourshahian, S.; Limbach, P.A.; Janssen, G.R. “Ribosome Binding to Leaderless mRNA through Recognition of their 5’-terminal AUG”, RNA 14 (2008) 2159-2169. PMCID: PMC2553737.

Durairaj, A.; Limbach, P.A. “Mass Spectrometry of the Fifth Nucleoside: A Review of the Identification of Pseudouridine in Nucleic Acids”, Analytica Chimica Acta 623 (2008) 117-125. PMCID: PMC2597214.

Pourshahian, S.P.; Limbach, P.A. “Application of Delta Mass for the Identification of Peptide:Oligonucleotide Cross-links by Mass Spectrometry”, Journal of Mass Spectrometry 42 (2008) 1081-1088.

Menezes, S.; Gaston, K.W.; Krivos, K.V.; Apolinario, E.; Reich, N.; Sowers, K.; Limbach, P.A.; Perona, J.; “Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14”, Nucleic Acids Research 39 (2011) 7641-55. PMID: 21693558

Addepalli, B.; Limbach, P.A.;  “Mass Spectrometry-Based Quantification of Pseudouridine in RNA”, Journal of the American Society for Mass Spectrometry 22 (2011) 1363-1372. PMID: 21953190.

Wetzel, C.; Limbach, P.A.; “Global Identification of Transfer RNAs by Liquid Chromatography-Mass Spectrometry”, Journal of Proteomics, 75 (2012) 3450-3464.  (Invited article for Special Issue on Genome Regulation).More Information

Phillips, G.; Swairjo, M.A.; Gaston, K.W.; Bailly, M.; Iwata-Reuyl, D.; Limbach, P.A.; de Crecy-Lagard, V.; “Diversity of Archaeosine Synthesis in Crenarchaeota”, ACS Chemical Biology, 7 (2012) 300-305.More Information

Krivos, K.L.; Addepalli, B.; Limbach, P.A.; “Removal of 3’ Phosphate Group by Bacterial Alkaline Phosphatase Improves Sequence Coverage of RNase Digestion Products Analyzed by Collision Induced Dissociation Mass Spectrometry” Rapid Communications in Mass Spectrometry, 25 (2011) 3609-3616.More Information

Catron, B.L.; Caruso, J.A.; Limbach, P.A.; “Selective Detection of Peptide-Oligonucleotide Heteroconjugates Utilizing Capillary HPLC-ICPMS”, Journal of the American Society for Mass Spectrometry. 23 (2012) 1053-1061.More Information

Easter, R.N.; Barry, C.G.; Caruso, J.A.; Limbach, P.A.; “Separation and Identification of Phosphorothioate Oligonucleotides by Hydrophilic Interaction Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry”, Analytical Methods 5 (2013) 2657-2659.More Information

Nayak, N.; Putnam, A.; Chen, T.; Schäfermeyer, K.R.; Addepalli, B.; Lowenson, J.; Patra, B.; Jankowsky, E.; Perry, S.E.; Dinkins, R.;  Limbach, P.A.; Ray, A.; Clarke, S.G.; Downie, B.; “The DEAD-box RNA helicase, PRH75, is susceptible to isoAsp formation but can be repaired and activity restored by PROTEIN ISOASPARTYL METHYLTRANSFERASE”, The Plant Cell 25 (2013) 2573-86.More Information

Butterer, A.; Zorc, M.; Castleberry, C.M.; Limbach, P.A.; “Using Immobilized Enzymes to Reduce RNase Contamination in RNase Mapping of Transfer RNAs by Mass Spectrometry”, Analytical and Bioanalytical Chemistry, 402 (2012) 2701-2711.More Information

Aldinger, C.A.; Leisinger, A-K; Gaston, K.W.; Limbach, P.A.; Igloi, G.L.; “The absence of A-to-I editing in the anticodon of plant cytoplasmic tRNAArgACG demands a relaxation of the wobble rules in plants”, RNA Biology 9 (2012) 1-8.

Li, S.; Limbach, P.A.; “Method for Comparative Analysis of Ribonucleic Acids Using Isotope Labeling and Mass Spectrometry” Analytical Chemistry 84 (2012) 8607-8613.More Information

Lokits, K.E.; Limbach, P.A.; Caruso, J.A. “Interfaces for Capillary LC with ICPMS Detection: A Comparison of Nebulizers/Spray Chamber Configurations”, J. Anal. At. Spectrom. 24 (2009) 528-534

Frankel, L.K.; Sallans, L.; Limbach, P.A.; Bricker, T.M.; “Identification of Oxidized Amino Acid Residues in the Vincinity of the Mn4CaO5 Cluster of Photosystem II: Implications for the Identification of Oxygen Channels within the Photosystem”, Biochemistry 51 (2012) 6371-6377.More Information

Guy, M.P.; Podyma, B.M.; Preston, M.A.; Shaheen, H.H.; Krivos, K.L.;Limbach, P.A.; Hopper, A.K.; Phizicky, E.M.; “Distinct Trm7 complexes that direct anticodon loop modifications are required for efficient tRNAPhe function and healthy growth in yeast”, RNA 18 (2012) 1921-1933.More Information

Russell, S.P.; Limbach, P.A.; “Evaluating the Reproducibility of Quantifying Modified Nucleosides from Ribonucleic Acids by LC-UV-MS” Journal of Chromatography, B 923–924 (2013) 74-82.More Information

Wong, S.Y.; Javid, B.; Addepalli, B.; Piszczek, G.; Strader, M.B.; Limbach, P.A.; Barry III, C.E. “Functional Role of Methylation of G518 of the 16S rRNA 530 loop by GidB in Mycobacterium tuberculosisAntimicrobial Agents and Chemotherapy 57 (2013) 6311-6318.More Information

Li, S.; Limbach, P.A.; “Comparative Sequencing of Transfer Ribonucleic Acids by Isotope Labeling and Mass Spectrometry”, Analyst 138 (2013) 1386-1394.More Information

Frankel, L.K.; Sallans, L.; Limbach, P.A.; Bricker, T.M.; “Oxidized Amino Acid Residues in the Vicinity of QA and PheoD1 of the Photosystem II Reaction Center: Putative Generation Sites of Reducing-Side Reactive Oxygen Species” PLoS One. 8 (2013) e58042.More Information

Frankel, L.K.; Sallans, L.; Bellamy, H.; Goettert, J.S.; Limbach, P.A.; Bricker, T.M.; “Radiolytic Mapping of Solvent Contact Surfaces in Photosystem II of Higher Plants: Experimental Identification of Putative Water Channels within the Photosystem” Journal of Biological Chemistry 288 (2013) 23565-23572.More Information

Rodriguez-Hernandez, A.; Spears, J.; Gaston, K.W.; Limbach, P.A.; Gamper, H.; Hou, Y.-M.; Agris, P.; Perona, J.J.; “Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position” Journal of Molecular Biology 425 (2013) 3888-3906.More Information

Wetzel, C.; Limbach, P.A.; “The Global Identification of tRNA Isoacceptors by Targeted Tandem Mass Spectrometry”, Analyst 138 (2013) 6063-6072.More Information

Clatterbuck Soper, S.F.; Dator, R.P.; Limbach, P.A.; Woodson, S.A.; “In vivo X-ray footprinting of pre-30S ribosomes reveals chaperone-dependent remodeling of late assembly intermediates”, Molecular Cell 52 (2013) 506-516.More Information

Köhrer, C.; Mandal, D.; Gaston, K.W.; Grosjean, H.; Limbach, P.A.; RajBhandary, U.L.; “Life without tRNAIle-lysidine synthetase: Translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical tRNA2Ile“ Nucleic Acids Research  42 (2014) 1904-1915.

Wetzel, C.; Li, S.; Limbach, P.A.; “Metabolic De-isotoping for Improved LC-MS Characterization of Modified RNAs” Journal of the American Society for Mass Spectrometry 25 (2014) 1114-1123.

Dator, R.P.; Gaston, K.W.; Limbach, P.A.; “Multiple Enzymatic Digestions and Ion Mobility Separation Improve Quantification of Bacterial Ribosomal Proteins by Data Independent Acquisition Liquid Chromatography−Mass Spectrometry” Analytical Chemistry 86 (2014) 4264-4270.

Zallot, R.; Brochier-Armanet, C.; Gaston, K.W.; Forouhar, F.; Limbach, P.A.; Hunt, J.; de Crécy-Lagard, V.; “The plant, animal, and fungal micronutrient queuosine is salvaged by members of the DUF2419 protein family”, ACS Chemical Biology 9 (2014) 1812-1825.

Puri, P.; Wetzel, C.; Saffert, P.; Gaston, K.W.; Russell, S.P.; van der Vlies, P.; Zhang, G.; Limbach, P.A.; Ignatova, Z.; Poolman, B.; “Systematic identification tRNAome and its dynamics in Lactococcus lactis.” Molecular Microbiology 93 (2014) 944-956.

Li, S.; Limbach, P.A.; “Identification of RNA Sequence Isomers by Isotope Labeling and LC-MS/MS” Journal of Mass Spectrometry 49 (2014) 1191-1198.

Wilson, Z.; McGuire, S.; Limbach, P.A.; Doyle, M.; Marzilli, L.; Warner, I.M.; “Diversifying Science, Technology, Engineering, and Mathematics – An empirical inquiry into successful approaches in chemistry” Journal of Chemical Education 91 (2014) 1860–1866.

Mummadisettia, M.P.; Frankel, L.K.; Bellamy, H.; Sallans, L.; Goettertb, J.S.; Brylinski, M.; Limbach, P.A.; Bricker, T.M.; “Use of protein crosslinking and radiolytic footprinting to elucidate PsbP and PsbQ interactions within higher plant Photosystem II” Proceedings of the National Academy of Sciences, USA. 111 (2014) 16178-16183.

Gaston, K.W.; Limbach, P.A.; “Mass Spectrometry for the Identification and Characterization of Modified Nucleosides and Nucleic Acids” RNA Biology (2014) 11 1568-1585. PMID: 25616408.

Sample, P.J.; Gaston, K.W.; Alfonzo, J.D.; Limbach, P.A.; “RoboOligo: Software for Mass Spectrometry Data to Support Manual and De Novo Sequencing of Post-transcriptionally Modified Ribonucleic Acids” Nucleic Acids Research (2015) 43 e64. 

Houser, W.H.; Butterer, A.; Addepalli, B.; Limbach, P.A.; “Enzymatic Generation of 3’-Phosphate from 2’,3’-Cyclic Phosphate Endonuclease Digestion Products of Recombinant RNase U2 for Improved LC/MS Analysis of RNAs” Analytical Biochemistry (2015) 478 52-58. 

Sample, P. J. ; Kořený, L.; Paris, Z.; Gaston, K.W.; Rubio, M.A.T.; Fleming, I.M.C.; Hinger, S.; Horáková, E.; Limbach, P.A.; Lukeš, J.; Alfonzo, J.D.; “A common tRNA modification at an unusual location: the discovery of wyosine biosynthesis in mitochondria” Nucleic Acids Research (2015) 43 4262-4273. 

Li, M.; Addepalli, B.; Chen, Q.; Wang, W.; Limbach, P.A.; LaSalle, J.; Zeng, S.; Huang, M.; Yu, A-Y.; “Chimeric miR-1291 biosynthesized efficiently in E. coli is effective to reduce target gene expression in human carcinoma cells and improve chemosensitivity” Drug Metabolism and Disposition (2015) 43 1129-1136. 

Wang, W-P.; Ho, P-Y.; Chen, Q. Addepalli, B.; Limbach, P.A.; Li, M.; Wu, W.; Jilek, J.L.; Qiu, J.; Zhang, H.; Li, T.; Wun, T.; Lam, K.S.; Yu, A-Y.; “Bioengineering novel chimeric microRNA-34a for prodrug cancer therapy: High-yield expression and purification, and structural and functional characterization” The Journal of Pharmacology and Experimental Therapeutics (2015) 354 131-41. 

Addepalli, B.; Lesner N.P.; Limbach, P.A.; “Detection of RNA nucleoside modifications with the uridine-specific ribonuclease MC1 from Momordica charantiaRNA (2015) 21 1746-56. 

Cao, X.; Limbach, P.A.; “Enhanced Detection of Post-Transcriptional Modifications Using a Mass-Exclusion List Strategy for RNA Modification Mapping by LC-MS/MS” Analytical Chemistry (2015) 87 8433-40. 

Ross, R.; Sauter Jr., A.D.; Limbach, P.A.; “Induction Based Fluidics (IBF) for Droplet-Based Mass Spectrometric Analysis of Oligonucleotides” Journal of Mass Spectrometry (2015) 50 1175-1179. 

Thiaville, P.C.; El Yacoubi, B.; Köhrer, C.; Joseph-Thiaville, J.; Deutsch, C.; Iwata-Reuyl, D.; Bacusmo, J.M.; Armengaud, J.; Bessho, Y.; Wetzel, C.; Cao, X.; Limbach, P.A.; RajBhandary, U.L.; de Crécy-Lagard, V.; “Essentiality in Bacteria of threonylcarbamoyladenosine (t6A), a universal modification of tRNA” Molecular Microbiology (2015) 98 1199–1221

Book

Banoub, J.H.; Limbach, P.A. Mass Spectrometry of Nucleosides and Nucleic Acids; Taylor & Francis, Inc., 2010.

Review

Fabris, D, & Limbach, P A (2010). Review of the 22nd Sanibel Conference on mass spectrometry: from structural biology to drug discovery: new roles for mass spectrometry of nucleic acids. Journal of the American Society for Mass Spectrometry, 21(7), R1-4.

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Book Chapter

Hossain, M.; Limbach, P.A. Comparison of Matrices in MALDI Mass Spectrometry in Electrospray and MALDI Mass Spectrometry: Fundamentals, Instrumentation, Practicalities and Biological Applications, Cole, R. ed. (2010) John Wiley & Sons, Inc. Hoboken, New Jersey 215-244.

Coombs, C.C., Limbach, P.A. Electrospray Ionization Mass Spectrometry for the Direct Analysis of Modified Nucleosides in Small RNAs in Mass Spectrometry of Nucleosides and Nucleic Acids Banoub, J.H.; Limbach, P.A. eds (2010) Taylor & Francis, Inc., Boca Raton, Florida, 423-429.

Hossain, M., Limbach, P.A. (2010) Quantitative Identification of Nucleic Acids via Signature Digestion Products Detected Using Mass Spectrometry in Mass Spectrometry of Nucleosides and Nucleic Acids Banoub, J.H.; Limbach, P.A. eds (2010) Taylor & Francis, Inc., Boca Raton, Florida, 397-421.

Jin, L.; Rodicio, L.M.; Limbach, P.A. Principles of Proteomics in Angiogenesis, Functional and Medicinal Foods Losso, J.N.; Shahidi, F.; Bagchi, D., Ed(s); Marcel Dekker, Inc., (2007), pp 291-314.

Mecomber, J.S.; Dominick, W.D.; Jin, L.; Limbach, P.A. BioMEMS Devices for Proteomics in BioMEMS: Technologies and Applications Soper, S.A., Ed; Taylor & Francis, Inc., (2007).

Patteson, K.; Limbach, P.A. "Nucleic Acids: DNA and RNA Seqeuence Analysis". In Encyclopedia of Mass Spectrometry Volume 3: Biological Applications Part B: Carbohydrates, Nucleic A; Caprioli, R.; Gross, M., Ed(s); Elsevier, Inc., (2005), pp 1-10.

Patteson, K.; Limbach, P.A. "Nucleic Acids: Base Analysis". In Encyclopedia of Mass Spectrometry Volume 3: Biological Applications Part B: Carbohydrates, Nucleic A; Caprioli, R.; Gross, M., Ed(s); Elsevier, Inc., (2005), pp 14-18.

Macha, S.F.; Limbach, P.A. "Analysis of Polymeric Hydrocarbon Materials by Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry". In Analytical Advances in Hydrocarbon Research; Hsu, S., Ed(s); Kluwer Academic/Plenum Publishers: New York, N.Y., (2003), pp 385-404.

Castleberry, C.M.; Rodicio, L.P.; Limbach, P.A. Electrospray ionization mass spectrometry of oligonucleotides in Current protocols in nucleic acid chemistry Beaucage, S.L. [et al.] eds (2008), Chapter 10, Unit 10.2.

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Limbach, P.A.; Hossain, M.; “Matrix-Assisted Laser Desorption/Ionization” in VOLUME V. Bioanalytical Approaches: MS, NMR, Isotope Methods, Spectroscopy, Ligand-Binding, and Other Analytical Chemistry Methods Muddiman, D.C. and Cohen, L. eds Encyclopedia of Drug Metabolism and Interactions (2012) John Wiley & Sons, Inc.More Information

Cao X., and Limbach P.A., (2015) Mass Spectrometry: Nucleic Acids and Nucleotides Studied Using MS. In: Reedijk, J. (Ed.) Elsevier Reference Module in Chemistry, Molecular Sciences and Chemical Engineering. Waltham, MA: Elsevier. 30-Nov-2015 doi:10.1016/B978-0-12-409547-2.11691-9.

In Press

Wetherill, Yelena B, Hess-Wilson, Janet K, Comstock, Clay E S, Shah, Supriya A, Buncher, C Ralph, Sallans, Larry, Limbach, Patrick A, Schwemberger, Sandy, Babcock, George F, & Knudsen, Karen E (2006). Bisphenol A facilitates bypass of androgen ablation therapy in prostate cancer. Molecular cancer therapeutics, 5(12), 3181-90.

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McLachlan, Anne, Borchers, Michael, Velayutham, Prakash, Wagner, Michael, & Limbach, Patrick A (2006) Characterizing the reproducibility of a protein profiling method for the analysis of mouse bronchoalveolar lavage fluid. Journal of proteome research, 5(11), 3059-65.

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Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2006). Unlimited-volume electrokinetic stacking injection in sweeping capillary electrophoresis using a cationic surfactant. Analytical chemistry, 78(17), 6035-42.

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Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2006). On-line sample preconcentration by sweeping with dodecyltrimethylammonium bromide in capillary zone electrophoresis. Journal of chromatography. A, 1125(2), 263-9.

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Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, Arias, Francisco, & Heineman, William R (2006). On-line sample preconcentration using field-amplified stacking injection in microchip capillary electrophoresis. Analytical chemistry, 78(11), 3730-7.

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Meng, Zhaojing, & Limbach, Patrick A (2006). Mass spectrometry of RNA: linking the genome to the proteome. Briefings in functional genomics & proteomics, 5(1), 87-95.

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Carr, Jennifer F, Hamburg, Daisy-Malloy, Gregory, Steven T, Limbach, Patrick A, & Dahlberg, Albert E (2006). Effects of streptomycin resistance mutations on posttranslational modification of ribosomal protein S12. Journal of bacteriology, 188(5), 2020-3.

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Mecomber, Justin S, Stalcup, Apryll M, Hurd, Doug, Halsall, H Brian, Heineman, William R, Seliskar, Carl J, Wehmeyer, Kenneth R, & Limbach, Patrick A (2006). Analytical performance of polymer-based microfluidic devices fabricated by computer numerical controlled machining. Analytical chemistry, 78(3), 936-41.

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Piruska, Aigars, Nikcevic, Irena, Lee, Se Hwan, Ahn, Chong, Heineman, William R, Limbach, Patrick A, & Seliskar, Carl J (2005). The autofluorescence of plastic materials and chips measured under laser irradiation. Lab on a chip, 5(12), 1348-54.

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Suh, Moo-Jin, Hamburg, Daisy-Malloy, Gregory, Steven T, Dahlberg, Albert E, & Limbach, Patrick A (2005). Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry. Proteomics, 5(18), 4818-31.

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Meades, Glen D, McLachlan, Anne, Sallans, Larry, Limbach, Patrick A, Frankel, Laurie K, & Bricker, Terry M (2005). Association of the 17-kDa extrinsic protein with photosystem II in higher plants. Biochemistry, 44(46), 15216-21.

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Currie, Christa A, Heineman, William R, Halsall, H Brian, Seliskar, Carl J, Limbach, Patrick A, Arias, Francisco, & Wehmeyer, Kenneth R (2005). Estimation of pK(a) values using microchip capillary electrophoresis and indirect fluorescence detection. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 824(1-2), 201-5.

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Tummala, Rama, Green-Church, Kari B, & Limbach, Patrick A (2005). Interactions between sodium dodecyl sulfate micelles and peptides during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of proteolytic digests. Journal of the American Society for Mass Spectrometry, 16(9), 1438-46.

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Tu, Jian, Halsall, H Brian, Seliskar, Carl J, Limbach, Patrick A, Arias, Francisco, Wehmeyer, Kenneth R, & Heineman, William R (2005). Estimation of logP(ow) values for neutral and basic compounds by microchip microemulsion electrokinetic chromatography with indirect fluorimetric detection (muMEEKC-IFD). Journal of pharmaceutical and biomedical analysis, 38(1), 1-7.

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Meng, Zhaojing, & Limbach, Patrick A (2005). Quantitation of ribonucleic acids using 18O labeling and mass spectrometry. Analytical chemistry, 77(6), 1891-5.

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Meng, Zhaojing, & Limbach, Patrick A (2005). Shotgun sequencing small oligonucleotides by nozzle-skimmer dissociation and electrospray ionization mass spectrometry. European journal of mass spectrometry (Chichester, England), 11(2), 221-9.

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Cameron, Dale M, Gregory, Steven T, Thompson, Jill, Suh, Moo-Jin, Limbach, Patrick A, & Dahlberg, Albert E (2004). Thermus thermophilus L11 methyltransferase, PrmA, is dispensable for growth and preferentially modifies free ribosomal protein L11 prior to ribosome assembly. Journal of bacteriology, 186(17), 5819-25.

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Meng, Zhaojing, Simmons-Willis, Tracey A, & Limbach, Patrick A (2004). The use of mass spectrometry in genomics. Biomolecular engineering, 21(1), 1-13.

Suh, Moo-Jin, & Limbach, Patrick A (2004). Investigation of methods suitable for the matrix-assisted laser desorption/ionization mass spectrometric analysis of proteins from ribonucleoprotein complexes. European journal of mass spectrometry (Chichester, England), 10(1), 89-99.

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Tummala, Rama, & Limbach, Patrick A (2004). Effect of sodium dodecyl sulfate micelles on peptide mass fingerprinting by matrix-assisted laser desorption/ionization mass spectrometry. Rapid communications in mass spectrometry : RCM, 18(18), 2031-5.

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Hassett, Daniel J, Limbach, Patrick A, Hennigan, Robert F, Klose, Karl E, Hancock, Robert E W, Platt, Mark D, & Hunt, Donald F (2003). Bacterial biofilms of importance to medicine and bioterrorism: proteomic techniques to identify novel vaccine components and drug targets. Expert opinion on biological therapy, 3(8), 1201-7.

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Berhane, Beniam T, & Limbach, Patrick A (2003). Stable isotope labeling for matrix-assisted laser desorption/ionization mass spectrometry and post-source decay analysis of ribonucleic acids. Journal of mass spectrometry : JMS, 38(8), 872-8.

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Dominick, Wendy D, Berhane, Beniam T, Mecomber, Justin S, & Limbach, Patrick A (2003). Covalent immobilization of proteases and nucleases to poly(methylmethacrylate). Analytical and bioanalytical chemistry, 376(3), 349-54.

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Berhane, Beniam T, & Limbach, Patrick A (2003). Functional microfabricated sample targets for matrix-assisted laser desorption/ionization mass spectrometry analysis of ribonucleic acids. Analytical chemistry, 75(9), 1997-2003.

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Robins, Chad, & Limbach, Patrick A (2003). The use of nonpolar matrices for matrix-assisted laser desorption/ionization mass spectrometric analysis of high boiling crude oil fractions. Rapid communications in mass spectrometry : RCM, 17(24), 2839-45.

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Chou, Chau-Wen, Limbach, Patrick A, & Cole, Richard B (2002). Fragmentation pathway studies of oligonucleotides in matrix-assisted laser desorption/ionization mass spectrometry by charge tagging and H/D exchange. Journal of the American Society for Mass Spectrometry, 13(12), 1407-17.

Limbach, Patrick A, & Meng, Zhaojing (2002). Integrating micromachined devices with modern mass spectrometry. The Analyst, 127(6), 693-700.

Macha, S F, Limbach, P A, Hanton, S D, & Owens, K G (2001). Silver cluster interferences in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of nonpolar polymers. Journal of the American Society for Mass Spectrometry, 12(6), 732-43.

Patteson, K G, Rodicio, L P, & Limbach, P A (2001). Identification of the mass-silent post-transcriptionally modified nucleoside pseudouridine in RNA by matrix-assisted laser desorption/ionization mass spectrometry. Nucleic acids research, 29(10), E49-9.

Polo, L M, & Limbach, P A (2001). Analysis of oligonucleotides by electrospray ionization mass spectrometry. Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage. [et al.], Chapter 10, Unit 10.2.

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Chou, C W, & Limbach, P A (2001). Analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage. [et al.], Chapter 10, Unit 10.1.

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Meng, Z, Qi, S, Soper, S A, & Limbach, P A (2001). Interfacing a polymer-based micromachined device to a nanoelectrospray ionization Fourier transform ion cyclotron resonance mass spectrometer. Analytical chemistry, 73(6), 1286-91.

Green-Church, K B, Limbach, P A, Freitas, M A, & Marshall, A G (2001). Gas-phase hydrogen/deuterium exchange of positively charged mononucleotides by use of Fourier-transform ion cyclotron resonance mass spectrometry. Journal of the American Society for Mass Spectrometry, 12(3), 268-77.

Macha, Limbach, & Savickas, (2000). Application of nonpolar matrices for the analysis of low molecular weight nonpolar synthetic polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of the American Society for Mass Spectrometry, 11(8), 731-7.

Ragas, J A, Simmons, T A, & Limbach, P A (2000). A comparative study on methods of optimal sample preparation for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization mass spectrometry. The Analyst, 125(4), 575-81.

Breaux, G A, Green-Church, K B, France, A, & Limbach, P A (2000). Surfactant-aided, matrix-assisted laser desorption/ionization mass spectrometry of hydrophobic and hydrophilic peptides. Analytical chemistry, 72(6), 1169-74.

Green-Church, K B, & Limbach, P A (2000). Mononucleotide gas-phase proton affinities as determined by the kinetic method. Journal of the American Society for Mass Spectrometry, 11(1), 24-32.

Vandell, V E, & Limbach, P A (1999). Polyamine co-matrices for matrix-assisted laser desorption/ionization mass spectrometry of oligonucleotides. Rapid communications in mass spectrometry : RCM, 13(20), 2014-21.

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Polo, L M, & Limbach, P A (1998). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the analysis of RNase H cleavage products. Journal of mass spectrometry : JMS, 33(12), 1226-31.

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Vandell, V E, & Limbach, P A (1998). Electrospray ionization mass spectrometry of metalloporphyrins. Journal of mass spectrometry : JMS, 33(3), 212-20.

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Mangroo, D, Limbach, P A, McCloskey, J A, & RajBhandary, U L (1995). An anticodon sequence mutant of Escherichia coli initiator tRNA: possible importance of a newly acquired base modification next to the anticodon on its activity in initiation. Journal of bacteriology, 177(10), 2858-62.

Limbach, P A, Crain, P F, & McCloskey, J A (1995). Characterization of oligonucleotides and nucleic acids by mass spectrometry. Current opinion in biotechnology, 6(1), 96-102.

Limbach, P A, Crain, P F, Pomerantz, S C, & McCloskey, J A (1995). Structures of posttranscriptionally modified nucleosides from RNA. Biochimie, 77(1-2), 135-8.

Limbach, P A, Crain, P F, & McCloskey, J A (1994). Summary: the modified nucleosides of RNA. Nucleic acids research, 22(12), 2183-96.

Castleberry, Colette M, Chou, Chau-Wen, & Limbach, Patrick A (2008). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligonucleotides. Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage. [et al.], Chapter 10, Unit 10.1.

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Durairaj, Anita, & Limbach, Patrick A (2008). Improving CMC-derivatization of pseudouridine in RNA for mass spectrometric detection. Analytica chimica acta, 612(2), 173-81.

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Gong, Maojun, Nikcevic, Irena, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2008). Protein-aptamer binding studies using microchip affinity capillary electrophoresis. Electrophoresis, 29(7), 1415-22.

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Platt, Mark D, Schurr, Michael J, Sauer, Karin, Vazquez, Gustavo, Kukavica-Ibrulj, Irena, Potvin, Eric, Levesque, Roger C, Fedynak, Amber, Brinkman, Fiona S L, Schurr, Jill, Hwang, Sung-Hei, Lau, Gee W, Limbach, Patrick A, Rowe, John J, Lieberman, Michael A, Barraud, Nicolas, Webb, Jeremy, Kjelleberg, Staffan, Hunt, Donald F, & Hassett, Daniel J (2008). Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions. Journal of bacteriology, 190(8), 2739-58.

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Mecomber, Justin S, Murthy, Rajesh S, Rajam, Sridhar, Singh, Pradeep N D, Gudmundsdottir, Anna D, & Limbach, Patrick A (2008). Photochemical functionalization of polymer surfaces for microfabricated devices. Langmuir : the ACS journal of surfaces and colloids, 24(7), 3645-53.

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Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2007). Flow manipulation for sweeping with a cationic surfactant in microchip capillary electrophoresis. Journal of chromatography. A, 1167(2), 217-24.

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Duderstadt, Randall E, Tsuie, Barbara M, Macha, Stephen F, & Limbach, Patrick A (2007). Investigation of single-site zirconium azaborolinyl complexes by laser desorption ionization time of flight mass spectrometry. Analytica chimica acta, 596(1), 124-31.

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Suh, Moo-Jin, Pourshahian, Soheil, & Limbach, Patrick A (2007). Developing limited proteolysis and mass spectrometry for the characterization of ribosome topography. Journal of the American Society for Mass Spectrometry, 18(7), 1304-17.

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Nikcevic, Irena, Lee, Se Hwan, Piruska, Aigars, Ahn, Chong H, Ridgway, Thomas H, Limbach, Patrick A, Wehmeyer, K R, Heineman, William R, & Seliskar, Carl J (2007). Characterization and performance of injection molded poly(methylmethacrylate) microchips for capillary electrophoresis. Journal of chromatography. A, 1154(1-2), 444-53.

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Gong, Maojun, Wehmeyer, Kenneth R, Stalcup, Apryll M, Limbach, Patrick A, & Heineman, William R (2007). Study of injection bias in a simple hydrodynamic injection in microchip CE. Electrophoresis, 28(10), 1564-71.

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Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2007). Frontal analysis in microchip CE: a simple and accurate method for determination of protein-DNA dissociation constant. Electrophoresis, 28(5), 837-42.

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Hossain, Mahmud, & Limbach, Patrick A (2007). Mass spectrometry-based detection of transfer RNAs by their signature endonuclease digestion products. RNA (New York, N.Y.), 13(2), 295-303.

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Prefaces

Lubman, D.M.; Limbach, P.A. "Mass spectrometry. Preface." Analytica Chimica Acta, 627(1), (2008) 1-2.

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Presentations

Invited Presentations

(11-2007. ) Modified RNAs: From Cancer to Biochemistry .Bethany College, Department of Chemistry.

(03-2007. ) Dissecting RNAs and RNPs in the Gas Phase .Brown University, Department of Microbiology and Biochemistry.

(01-2007. ) Mass Spectrometry as a Tool to Study RNA and RNA:Protein Complexes .Rennselaer Polytechnic Institute, Department of Chemistry.

(11-2006. ) Optimizing Surface Chemistries to Develop Plastic Microfluidic and Microarray Devices for Mass Spectrometry .Eastern Analytical Symposium,

(11-2005. ) RNA for the Masses .University of Cincinnati, Department of Biological Sciences.

(06-2005. ) Developing Mass Spectrometry Approaches for RNA and RNP Complexes .Ohio State University, Department of Molecular Virology, Immunology and Human Genetics.

(04-2005. ) Protein Factories on Bathroom Scales .John Carroll University, Department of Chemistry.

(11-2004. ) Microchip Mass Spectrometry: Plastics Make it Possible .Bradley University, Department of Chemistry.

(06-2004. ) Optimizing Surface Chemistries to Develop Plastic Microfluidic and Microarray Devices for Mass Spectrometry .87th Canadian Chemistry Conference and Exhibition,

(06-2004. ) Mass Spectrometry as a Tool for Probing Ribosome Structure .trometry as a Tool for Probing Ribosome Structure”, ,

(04-2004. ) Profiling the Source of Proteins .University of Oregon, Institute of Molecular Biology.

(04-2004. ) Developing Mass Spectrometry Approaches for RNP Complexes .University of California, Riverside, Department of Chemistry.

(11-2003. ) RNA for the Masses: Using Mass Spectrometry to Characterize RNA Structure .2003 Rustbelt RNA Meeting,

(11-2003. ) Interfacing Microchips to Mass Spectrometry .NSF Workshop on Miniaturization and Mass Spectrometry,

(10-2003. ) Microchip Mass Spectrometry: Plastics Make it Possible .University of Maryland, Baltimore County, Department of Chemistry.

(10-2003. ) Profiling the Source of Proteins: Mass Spectrometry of the Ribosome .35th ACS Central Regional Meeting,

(09-2003. ) Bioassays and Molecular Characterization by Microchip CE and Microchip MS .226th ACS National Meeting,

(02-2003. ) Integrating Microfabricated Devices with Modern Mass Spectrometry .LabAutomation, Palm Springs, CA.

(01-2010. ) Quantitative Identification of Modified Nucleic Acids .ASMS Sanibel Conference, St. Pete Beach, FL. Level:International

(04-2010. ) Characterizing Modified Nucleic Acids Using Mass Spectrometry .Department of Chemistry Highlands Seminar Series, Virginia Tech University. Level:Department

(05-2010. ) Characterizing Modified Nucleic Acids Using Mass Spectrometry .Department of Chemistry, Queens Univ Kingston Ontario CANADA. Level:Department

(01-2011. ) RNA Spell Checking: Approaches for Identifying Editing and Modification Sites .Gordon-Kenan Research Seminar on Editing and Modification of RNA and DNA, Galveston Island Texas. Conference. . Level:International

(01-2011. ) Advanced Mass Spectrometry Studies of RNA Modification .Gordon Research Conference on RNA Editing and Modification, Galveston Island Texas. Level:International

(02-2011. ) What's Wrong with Plain old RNA? .Department of Chemistry, Northern Kentucky University. Level:Department

(02-2011. ) Fundamentals of Bioanalytical Mass Spectrometry .Department of Chemistry, Virginia Tech University. Level:Department

(04-2011. ) The Utility of Mass Spectrometry for Studying RNA Modification and Editing .Frontiers in Proteomics, Univ Massachusetts Medical School, Worcester MA. Other Institution. .

(05-2011. ) Ion Mobility Mass Spectrometry for Oligonucleotide Impurity Profiling .Nucleic Acids Technologies for Diagnostics: Analytical Methods and Validation, TIDES 2011, Boston MA. Professional Meeting. .

(11-2011. ) What's Wrong with Plain old RNA? .Department of Chemistry, Wittenberg University. Other Institution. .

(04-2012. ) Using Mass Spectrometry to Study RNA Modification .Department of Chemistry, Wayne State University. Other Institution. .

(05-2012. ) Analytical Characterization of Branched Oligonucleotides .TIDES 2012 Meeting, Las Vegas, NV. Professional Meeting. .

(05-2012. ) New Mass Spectrometry Assays for Identifying and Characterizing Modified RNAs .60th ASMS conference on Mass Spectrometry and Allied Topics, Vancouver, Canada. Professional Meeting. .

(01-2012. ) What’s Wrong with Plain Old RNA: Identifying Modified RNAs and Understanding their Health Significance .University of Louisville Department of Chemistry. Other Institution. .

(10-2012. ) RNA Spell-Checking: How to Know when RNA is Modified and Why .Indiana State University Department of Chemistry. Other Institution. .

(08-2012. ) Mass Spectrometry for the Identification of Natural and Synthetic Oligonucleotides .Food and Drug Administration, Cincinnati, OH. Workshop. .

(02-2013. ) RNA Spell-Checking: How to Know when RNA is Modified and Why .Department of Chemistry, Mt. St. Joseph College. Other Institution. .

(02-2013. ) Studying RNA Editing and Modification Using Mass Spectrometry .Chemical Biology Program, University of Minnesota. Other Institution. .

(04-2013. ) Mass Spectrometry for the Analysis of Modified RNAs and RNA-Protein Cross-links .RNA Center Speaker Series, The Ohio State University. Other Institution. .

(10-2013. ) High Performance Mass Spectrometry for Structural and Conformational Analysis of Natural and Therapeutic Oligonucleotides .Waters Corporation, Webinar Series. Other Institution. .

(04-2014. ) New Mass Spectrometry Assays for Identifying and Characterizing Modified RNAs .College of Pharmacy, University of Georgia. Other Institution. .

(04-2014. ) New Mass Spectrometry Assays for Identifying and Characterizing Modified RNAs .2014 ACS National Meeting, Dallas, TX. Professional Meeting. .

(05-2014. ) RNA Spell-Checking: How to Know when RNA is Modified and Why .Department of Chemistry, Ball State University. Other Institution. .

(05-2014. ) New Mass Spectrometry Assays for Identifying and Characterizing Modified RNAs .UC Davis Cancer Center, University of California, Davis. Other Institution. .

(10-2014. ) New Mass Spectrometry Assays for Identifying Modified RNAs .DFG Dnmt2 Meeting, Bonn, Germany. Conference. .

(10-2014. ) How to Keep Analytical Chemists from Drowning in Data .2014 Oesper Symposium, University of Cincinnati. UC. .

(02-2015. ) The Epitranscriptome: Challenges in Measuring Dynamic RNA Modifications .UC Department of Molecular Genetics, Biochemistry and Microbiology, Cincinnati, OH. UC. .

(03-2015. ) Exploring the Dynamics of tRNA Modification Patterns .Gordon Research Conference on RNA Editing and Modification, Lucca (Barga), Italy. Conference. .

(12-2015. ) Mapping and Measuring RNA Modifications by Mass Spectrometry .28th Annual Lake Louise Tandem Mass Spectrometry Workshop, Lake Louise Alberta, Canada. Workshop. .

Poster Presentations

M. Hossain, P.A. Limbach (10-2007. ) Large Scale Quantitive Analysis of Non-coding RNA (ncRNAs) by their Signature Digestion Products using Stable Isotope Labeling and MALDI-MS .NIH 2nd NGSRF 2007, Bethesda, MD. .

C.M Castleberry, L.Sallans, and P.A. Limbach (06-2007. ) Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS .55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN. .

D.-M Hamburg, M.-Suh and P.A. Limbach (06-2007. ) Conformational Changes and Chemical Reactivity of the Ribosome: An Application of Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN. .

M. Hossain, P.A. Limbach (06-2007. ) Global Quantification of tRNAs by their Signature Digestion Products and MALDI-MS .55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN. .

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (06-2007. ) Comparing Collision Induced Dissociation and Electron Capture Dissociation for the Sequencing of Peptide:Oligonucleotide Heteroconjugate .55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN. .

S. Pourshahian, P.A. Limbach (06-2007. ) Identification of Oligonucleotide-Peptide cross-links using mass defect .", 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN. .

R.L. Rohlfs, P.A. Limbach (06-2007. ) Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the Presence of Antibiotics .55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN. .

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (05-2007. ) Comparison of Collision Induced Dissociation and Electron Capture Dissociation for the Sequencing of Peptide:Oligonucleotide Heteroconjugate .Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY. .

R.L. Rohlfs, P.A. Limbach (05-2007. ) Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotics .Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY. .

S. Russell, P.A. Limbach (05-2007. ) Posttranscriptional Modifications in Bacterial rRNA and Heat Shock .Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY. .

C.M. Castleberry, L. Sallans, P.A. Limbach (03-2007. ) Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS .Graduate Poster Forum, University of Cincinnati, Cincinnati, OH. .

M. Hossain, P.A. Limbach (03-2007. ) Quantification of Large Ribosomal RNAs (rRNAs) in Escherichia coli .Graduate Poster Forum, University of Cincinnati. .

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2007. ) Implementing Electron Capture Dissociation as a Method of the Assessment of Cross Linked Protein-Nucleic Acid Complexes .Graduate Poster Forum, University of Cincinnati, Cincinnati, OH. .

R.L. Rohlfs, P.A. Limbach (03-2007. ) Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotic .Graduate Poster Forum, University of Cincinnati, Cincinnati, OH. .

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2007. ) Comparing Collision Induced Dissociation and Electron Capture dissociation for the Assessment of RNA-Peptide Complexes .Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH. .

D.-M Hamburg, D.D. Richardson, P.A. Limbach and J.A. Caruso (02-2007. ) Investigation of Metals in Ribosomal Proteins .European Winter Plasma Conference, Taormino, Italy. .

M. Hossain, C. Cherenfant, P.A. Limbach (02-2007. ) Detection of Cytosolic and Mitochondrial Transfer RNAs of Saccharomyces by their Signature Digestion Products .Pittcon, Chicago, IL. .

S. Pourshahian, P.A. Limbach (02-2007. ) Application of Mass Defect to Identification of Oligonucleotide-Peptide Cross-links .Pittcon, Chicago, IL. .

A.Durairaj, P.A. Limbach (10-2006. ) Identifying Pseudouridines in RNA by Mass Spectrometry .2006 Rustbelt RNA Meeting, Mt. Sterling, OH. .

C.M. Castleberry L. Sallans, P.A. Limbach (10-2006. ) Quantitation of Isotopically Labeled RNA Using LC-MS .2006 Rustbelt RNA Meeting, Mt. Sterling, OH. .

D.-M Hamburg, M.-J. Suh and P.A. Limbach (10-2006. ) Probing the Topography Ribonucleoprotein Complexes Using Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .2006 Rustbelt RNA Meeting, Mt. Sterling, OH. .

M. Hossain, P.A. Limbach (10-2006. ) Detection of Mitochondrial Transfer RNAs Import of Saccharomyces Cerevisiae by their Signature Digestion Products .2006 Rustbelt RNA Meeting, Mt. Sterling, OH. .

S. Russell, P.A. Limbach (10-2006. ) Posttranscriptional Modifications in Bacterial rRNA and Heat Shock .2006 Rustbelt RNA Meeting, Mt. Sterling, OH. .

C.M. Castleberry, L. Sallans, P.A. Limbach (10-2006. ) Quantitation of Isotopically Labeled RNA Using LC-MS .Oesper Symposium, University of Cincinnati, Cincinnati, OH. .

D.-M Hamburg, M.-J. Suh and P.A. Limbach (10-2006. ) Probing the Topography Ribonucleoprotein Complexes Using Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .Oesper Symposium, University of Cincinnati, Cincinnati, OH. .

Kady L. Krivos, Larry Sallans, Patric A. Limbach (10-2006. ) Implementing Electron Capture Dissociation as a Method of the Assessment of Cross Linked Protein-Nucleic Acid Complexes .Oesper Symposium, University of Cincinnati, Cincinnati, OH. .

R.L. Rolfs, P.A. Limbach (10-2006. ) Posttranslational and Posttranscriptional Modifications to the Large Ribosomal Subunit of Escherichia coli Grown in the Presence of Antibiotic .Oesper Symposium, University of Cincinnati, Cincinnati, OH. .

M. Hossain, P.A. Limbach (10-2006. ) Detection of Mitochondrial Transfer RNAs Import of Saccharomyces Cerevisiae by their Signature Digestion Products .2006 Oesper Symposium, University of Cincinnati. .

A.Durairaj, P.A. Limbach (06-2006. ) Optimizing a Mass Spectrometry-Based Approach for the Detection of CMC-derivatized Pseudouridine in RNA .54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA. .

D.-M Hamburg, M.-J. Suh and P.A. Limbach (05-2006. ) Limited Proteolysis and Matrix-assisted Laser Desorption/Ionization Mass Spectrometry for the Interpretation of Ribosomal Subunit Topography .54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA. .

M. Hossain, P.A. Limbach (05-2006. ) Signature Digestion Product of Transfer RNA’s Advantages of Multiple Ribonucleases .54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA. .

S. Macha, S. Russell, L. Sallans, and P.A. Limbach (05-2006. ) Evaluation of In-gel Protein Digestion protocols: How Important are the Steps Involved? .54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA. .

S. Pourishahian, P.A. Limbach (05-2006. ) Characterization of Ribosomes by Chemical Cross-linking and Mass Spectrometry .54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA. .

C.M Castleberry, P.A. Limbach (03-2006. ) Developing an LC-MS Approach for Quantifying RNAs .University of Cincinnati Graduate Poster Forum, Cincinnati, OH. .

D.-M Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (03-2006. ) Adventure Ribosomes Using Matrix-Assisted Laser desorption/Ionization Time-of-Flight Spectometry .University of Cincinnati Graduate Poster Forum, Cincinnati, OH. .

M. Hossain P.A. Limbach (03-2006. ) Augmenting Transfer Ribonucleic Acids (tRNAs) Escherichia coli by using Multiple Endoribonucleases .University of Cincinnati Graduate Poster Forum, Cincinnati, OH. .

D.-M Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (03-2006. ) Adventures with Ribosomes Using Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry .American Chemical Society National Meeting, Atlanta, GA. .

I.Nikcevic, S-H. Lee, A. Piruska, C. H. Ahn, P.A. Limbach, W. R. Heineman, C. J. Seliskar (01-2006. ) Characterization of Cyclic Olefin Copolymer (COC) and Poly(methylmethacrylate) (PMMA) Microchips for Capillary Electrophoresis .10th LabAutomation 2006, Palm Springs, CA. .

C. Coombs, P.A. Limbach (10-2005. ) Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. .

A.L. Dupre, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (10-2005. ) Polymer Microchips for Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS) .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. .

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (10-2005. ) Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. .

M. Hossain, P.A. Limbach (10-2005. ) On the Heels of Signature Digestion Products: tRNA Treasure Hunting .Oesper Symposium, University of Cincinnati. .

S. Pourshahian, M.-J. Suh, P.A. Limbach (10-2005. ) Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry .Oesper Symposium, University of Cincinnati. .

C. M. Castleberry, P.A. Limbach (10-2005. ) Developing an LC-MS Approach for Quantifying RNAs .2005 Rustbelt RNA Meeting, Deer Creek State Park & Resort, Mt. Sterling, OH. .

C. M. Castleberry, P.A. Limbach (10-2005. ) Developing an LC-MS Approach for Quantifying RNAs .Oesper Symposium, University of Cincinnati, Cincinnati, OH. .

C. Coombs, P.A. Limbach (10-2005. ) Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs .2005 Rustbelt RNA Meeting, Mt. Sterling, OH. .

A.Durairaj, P.A. Limbach (10-2005. ) Mapping Pseudouridines in RNA by MALDI Spectrometry .2005 Rustbelt RNA Meeting, Mt. Sterling, OH. .

C. Coombs, P.A. Limbach (05-2005. ) Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs .American Society for Mass Spectrometry, San Antonio, TX. .

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (05-2005. ) Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .American Society for Mass Spectrometry, San Antonio, TX. .

S. Pourshahian, M.-J. Suh, P.A. Limbach (05-2005. ) Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry .American Society for Mass Spectrometry, San Antonio, TX. .

A.L. Dupre, W.D. Dominick, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (03-2005. ) EOF Characterization of Surface-Modified PMMA Microchips for Use With Electrospray Ionization Mass Spectrometry .Graduate Poster Forum, University of Cincinnati, Cincinnati, OH. .

M. Hossain, P.A. Limbach (03-2005. ) A Facile Method for Detection and Quantification of Escherichia coli tRNAs by MALDI-MS .Graduate Poster Forum, University of Cincinnati, Cincinnati, OH. .

A. Durairaj, K.G. Patteson, P.A. Limbach (11-2004. ) Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA .2004 Rustbelt RNA Meeting,

S. Pourshahian, M.-J. Suh, P.A. Limbach (11-2004. ) Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry .2004 Rustbelt RNA Meeting,

P.A. Limbach, M. Hossain, Z. Meng, S. Pourshahian, M.J. Suh (06-2004. ) Mass Spectrometry as a Tool for Probing Ribosome Structure .Ninth Annual Meeting of the RNA Society,

A. Dupre, W.D. Dominick, J. Mecomber, H.B. Halsall, W.R. Heineman, C.J. Seliskar, K.R. Wehmeyer, P.A. Limbach (05-2004. ) EOF Characterization of Surface-Modified PMMA Microchips .ALA Lab Fusion,

C. Robins, P.A. Limbach (05-2004. ) Initial Ion Velocities During Charge-Transfer Ionization in MALDI-MS .52nd ASMS Conference on Mass Spectrometry and Allied Topics,

A. Durairaj, P.A. Limbach (05-2004. ) Towards Affinity Purification and Mass Spectrometric Analysis of Pseudouridines in RNA .52nd ASMS Conference on Mass Spectrometry and Allied Topics,

Z, Meng, M. Hossain, P.A. Limbach (05-2004. ) Modification Mapping, Sequencing and Quantitation of Nucleic Acids .52nd ASMS Conference on Mass Spectrometry and Allied Topics,

M. Tummala, L.K. Frankel, T.M. Bricker, P.A. Limbach (05-2004. ) Determining Protein-Protein Interactions in the Oxygen-evolving Complex (OEC) of Photosystem II by Mass Spectrometry .52nd ASMS Conference on Mass Spectrometry and Allied Topics,

S.B. Jayasinghe, S. Mounicou, T.D. Grant, P.A. Limbach, J.A. Caruso (05-2004. ) Investigation of Metalloproteins in Soy Bean Proteome by Chromatographic and Mass Spectrometric Techniques .52nd ASMS Conference on Mass Spectrometry and Allied Topics,

L. Jin, M. Sperling, A. Pesce, M. Wagner, P.A. Limbach (05-2004. ) Exploring MALDI-MS for Biomarker Screening of Colon Cancer .52nd ASMS Conference on Mass Spectrometry and Allied Topics,

J. Mecomber, D. Hurd, P.A. Limbach (02-2004. ) Rapid and Cost-Effective Prototyping of Plastic Microfabricated Devices for Mass Spectrometry .Lab Automation 2004,

A.Durairaj, K.G. Patteson, P.A. Limbach (11-2003. ) Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA .2003 Rustbelt RNA Meeting,

M.J. Suh, S. Pourshahian, P.A. Limbach (11-2003. ) Developing Cross-Links and MALDI-TOFMS Approaches for Exploring Ribosomal Assembly and Interactions .2003 Rustbelt RNA Meeting,

P.A. Limbach, K.G. Patteson, A. Durairaj (06-2003. ) Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNAs .51st ASMS Conference on Mass Spectrometry and Allied Topics,

Z. Meng, P.A. Limbach (06-2003. ) Shotgun Sequencing of Modified RNAs by Nozzle-Skimmer ESI-MS .51st ASMS Conference on Mass Spectrometry and Allied Topics,

J.S. Mecomber, P.A. Limbach (06-2003. ) Rapid and Cost-effective Prototyping of Plastic Microfabricated Devices for Mass Spectrometry .51st ASMS Conference on Mass Spectrometry and Allied Topics,

R. Tummala, K.B. Green-Church, P.A. Limbach (05-2003. ) Effect of Sodium Dodecyl Sulfate on MALDI-MS Analysis of Proteolytic Digests .”, 51st ASMS Conference on Mass Spectrometry and Allied Topics,

M.-J. Suh, P.A. Limbach (05-2003. ) Developing a MALDI Mass Spectrometry Approach for Probing Ribosomal Assembly and Interactions .51st ASMS Conference on Mass Spectrometry and Allied Topics,

W.D. Dominick, P.A. Limbach (05-2003. ) Polymethylmethacrylate (PMMA) Microfabricated Devices for Mass Spectrometry .51st ASMS Conf. on Mass Spectrometry and Allied Topics,

W.D. Dominick, P.A. Limbach (02-2003. ) Surface Modification of Polymethylmethacrylate (PMMA) Microfabricated Devices for Improved Protein Characterization .Lab Automation,

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2008. ) (In Press. ) “Investigation of electron capture dissociation (ECD) as an alternative to collision induced dissociation (CID) for the sequencing of peptide:oligonucleotide heteroconjugates” .Pittcon 2008, New Orleans, LA. . Professional Meeting. . Level:National

C.M. Castleberry, L. Sallans, R. Kepler, P.A. Limbach (03-2008. ) (In Press. ) “LC-MS/MS Analysis of Phosphorothioate Oligonucleotides” .Pittcon 2008, New Orleans, LA. . Professional Meeting. . Level:National

C.M. Castleberry, L. Sallans, R. Kepler, P.A. Limbach (03-2008. ) (In Press. ) “LC-MS/MS Analysis of Phosphorothioate Oligonucleotides” .Fifth Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH. . Conference. . Level:Regional

Kady L. Krivos, Patrick A. Limbach (03-2008. ) (In Press. ) “Electron Capture Dissociation for RNA: Protein Cross-links” .Fifth Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH. . Conference. . Level:Regional

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (06-2008. ) (In Press. ) “The Optimal Properties of Cross-linked RNA:Protein for Electron Capture Dissociation” .56th ASMS Conference on Mass Spectrometry and Allied Topics , Denver, CO. . Professional Meeting. . Level:International

C.M. Castleberry, R. Kepler, L. Sallans, R.M. Selby, P.A. Limbach (06-2008. ) (In Press. ) “Developing Approaches for Quantitative LC-MS/MS of Nucleic Acids” .56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO. . Professional Meeting. . Level:International

Susan P. Russell and Patrick A. Limbach (06-2008. ) (In Press. ) “Post-transcriptional Modifications in Bacterial rRNA and Heat Shock, A Reproducibility Study” .56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO. . Professional Meeting. . Level:International

Tiffany Hilson, Patrick A. Limbach (06-2008. ) (In Press. ) "Fractionation of Escherichia coli tRNAs Through Two Dimensional Polyacrylamide Gel Electrophoresis", .First Year Graduate Student Poster Session, Cincinnati, OH. . UC. . Level:Department

R. L. Rohlfs, P. A. Limbach (10-2008. ) (In Press. ) “Ribosome Modifications and Assembly Defects” .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

Kady L. Krivos, Patrick A. Limbach (10-2008. ) (In Press. ) “Electron Transfer Dissociation of RNA: Peptide synthetic cross-links” .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

Tiffany Hilson, Patrick A. Limbach (10-2008. ) (In Press. ) "Fractionation and Extraction of Escherichia coli tRNAs for Quantitative Signature Digestion Product Identification" .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Conference. . Level:Regional

Balasubrahmanyam Addepalli, Pavanapuresan Vaidyanathan, Arun Malhotra, Patrick A. Limbach (10-2008. ) (In Press. ) “Detection of Pseudouridine Modifications in Helix 69 of Escherichia coli 23S rRNA” .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Conference. . Level:Regional

R. L. Rohlfs, P. A. Limbach (10-2008. ) (In Press. ) “Ribosome Modifications and Assembly Defects” .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Conference. . Level:Regional

Susan P. Russell and Patrick A. Limbach (10-2008. ) (In Press. ) “ Heat Shock and Post-Transcriptional Modifications in Bacterial Ribosomal RNA” .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Conference. . Level:Regional

Kirk Gaston, Kady Krivos, Mary Anne T. Rubio, Patrick A. Limbach and Juan Alfonzo (01-2009. ) (In Press. ) “Editing and modification of Kineotoplastid threonyl-tRNA” .RNA Editing Gordon Conference, Galveston, TX. . Conference. . Level:International

I. Nikcevic, T. Wyrzykiewicz, P. A. Limbach (01-2009. ) (In Press. ) LC-MS method development for the characterization of low level impurities in modified phosphorothioatediesters oligonucleotides synthesized using solid-phase phosphoramidite approach .13th Lab Automation National Meeting 2009, Palms Springs, CA. . Professional Meeting. . Level:National

J. A. Caruso, R. N. Easter, K. E. Lokits. P. A. Limbach (03-2009. ) (In Press. ) “Elemental and molecular mass spectrometries for low-level detection of phosphorothioate antisense drugs” .The Pittsburgh Conference, Pittcon, Chicago, IL. . Conference. . Level:National

B. Addepalli, P. Vaidyanathan, A. Malhotra, P.A. Limbach (03-2009. ) (In Press. ) “Detection of Pseudouridine Modifications in Helix 69 of Escherichia coli 23S rRNA” .The Pittsburgh Conference, Pittcon, Chicago, IL. . Conference. . Level:National

R. N. Easter, P A. Limbach and J.A.Caruso (03-2009. ) (In Press. ) “Separation and identification of polythymidilic acids by hydrophilic interaction liquid chromatography (HILIC) coupled to ICPMS” .The Pittsburgh Conference, Pittcon, Chicago, IL. . Conference. . Level:National

I. Nikcevic, T. Wyrzykiewicz, P. A. Limbach (03-2009. ) (In Press. ) Method development for profiling of impurities in synthetic oligonucleotides synthesized by solid-phase phosphoramidite approach .The Pittsburgh Conference, Pittcon, Chicago, IL. . Conference. . Level:National

I. Nikcevic, T. Wyrzykiewicz, P. A. Limbach (04-2009. ) (In Press. ) Characterization of low level impurities in modified phosphorothioatediesters oligonucleotides synthesized using LC-FTMS .7th North American FTMS Conference, Key West, FL. . Conference. . Level:National

Kady Krivos and Patrick Limbach (04-2009. ) (In Press. ) ECD vs. ETD Sequencing of RNA: Protein Complexes .7th North American FTMS Conference, Key West, FL. . Conference. . Level:National

B. Catron, S. Afton, P. Limbach, J. Caruso (04-2009. ) (In Press. ) Advances in Sulfur and Phosphorus Detection via ICPMS: Novel Applications .Sixth Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH. . Conference. . Level:Regional

Kady Krivos and Patrick A. Limbach (04-2009. ) (In Press. ) Using Mass Spectrometry to Investigate tRNA Modifications .2009 Industrial Affiliates Meeting at UC, Cincinnati, OH. . UC. . Level:Department

Rebecca L. Rohlfs, Steven Gregory, Al E. Dahlberg, Patrick. A. Limbach (05-2009. ) (In Press. ) “Ribosome Modifications and Assembly Defects” .Fourteenth Annual Meeting of the RNA Society, Madison, WI. . Professional Meeting. . Level:International

S. Russell, P. A. Limbach (05-2009. ) (In Press. ) Measuring Global Changes in Post-transcriptionally Modified Nucleosides Induced by Heat Shock .Fourteenth Annual Meeting of the RNA Society, Madison, WI. . Professional Meeting. . Level:International

R. N. Easter, K. K. Kroening, K. E. Lokits, P. A. Limbach and J. A. Caruso (06-2009. ) (In Press. ) Separation and Quantification of antisense oligonucleoides by hydrophilic interaction liquid chromatography coupled to ICP-MS .57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA. . Conference. . Level:National

B. Addepalli, A.G. Hunt, P.A. Limbach (06-2009. ) (In Press. ) Using Mass Spectrometry to Characterize the Novel Disulfide Linkage in the Endonuclease Motif of Arabidopsis CPSF30 Ortholog .57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA. . Conference. . Level:National

Kady Krivos and Patrick A. Limbach (06-2009. ) (In Press. ) "CID vs. ECD/ETD Sequencing of RNA:Protein Complexes" .57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA. . Conference. . Level:National

R.P. Dator, R. L. Rohlfs, P.A. Limbach (06-2009. ) (In Press. ) Using Mass Spectrometry to Identify Components of Ribosome Assembly Intermediates .57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA. . Conference. . Level:National

Siwei Li, C.M. Castleberry, P.A. Limbach (06-2009. ) (In Press. ) Characterizing Quantifiable Signature Digestion Products of tRNAs by LC-MS/MS .57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA. . Conference. . Level:National

R. N. Easter, K. K. Kroening, K. E. Lokits, P. A. Limbach and J. A. Caruso (06-2009. ) (In Press. ) Separation and Quantification of antisense oligonucleoides by hydrophilic interaction liquid chromatography coupled to ICP-MS .2nd International Symposium on Metallomics, Cincinnati, OH. . Conference. . Level:International

B. Catron, S. Afton, P. Limbach, J. Caruso (06-2009. ) (In Press. ) Advances in Sulfur and Phosphorus Detection via ICPMS for the identification of oliogonucleotide: peptide heteroconjugates .2nd International Symposium on Metallomics, Cincinnati, OH. . Conference. . Level:International

A.G. Hunt, S. Rao, R.D. Dinkins, B. Addepalli, P.A. Limbach (08-2009. ) (In Press. ) Novel characteristics of the Arabidopsis polyadenylation factor subunit CPSF30 .2009 Cold Spring Harbor Laboratory Meeting on Eukaryotic mRNA Processing, Cold Spring Harbor, NY. . Professional Meeting. . Level:International

Nicki Reishus, Kady Krivos, Patrick A. Limbach (08-2009. ) (In Press. ) Incorporating increased amounts of 4-thiouridine into bacterial rRNA .University of Cincinnati 2009 REU Poster Session, Cincinnati, OH. . UC. . Level:Department

Annika Butterer, Colette M. Castleberry, Patrick A. Limbach (08-2009. ) (In Press. ) Immobilizing ribonucleases for qualitative and quantitative analysis of tRNAs .University of Cincinnati 2009 REU Poster Session, Cincinnati, OH. . UC. . Level:Department

B. Addepalli, A.G. Hunt, P.A. Limbach (10-2009. ) (In Press. ) Disulfide Linkage in the Endonuclease Motif of Arabidopsis CPSF30 Ortholog .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Professional Meeting. . Level:Regional

Susan Russell, Kady Krivos, Colette Castleberry, Patrick Limbach (10-2009. ) (In Press. ) Agmatidine is the Modified Cytidine in the Anticodon of tRNAIle in Archaea that Base-Pairs with Adenosine but not with Guanosine .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Professional Meeting. . Level:Regional

Rebecca L. Rohlfs, Romel Dator, Steven Gregory, Al E. Dahlberg, Patrick. A. Limbach (10-2009. ) (In Press. ) “Ribosome Modifications and Assembly Defects” .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Professional Meeting. . Level:Regional

T. D. Hilson, P.A. Limbach (10-2009. ) (In Press. ) Developing and Optimizing and a Mass Spec-Friendly Protocol for Fractionation of tRNAs Through Two-Dimensional Polyacrylamide Gel Electrophoresis .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Professional Meeting. . Level:Regional

R.P. Dator, R. L. Rohlfs, P.A. Limbach (10-2009. ) (In Press. ) Isolation and Characterization of Ribosome Assembly Intermediates .Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH. . Professional Meeting. . Level:Regional

Susan Russell, Kady Krivos, Colette Castleberry, Patrick Limbach (10-2009. ) (In Press. ) Agmatidine is the Modified Cytidine in the Anticodon of tRNAIle in Archaea that Base-Pairs with Adenosine but not with Guanosine .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

Rebecca L. Rohlfs, Romel Dator, Steven Gregory, Al E. Dahlberg, Patrick. A. Limbach (10-2009. ) (In Press. ) “Ribosome Modifications and Assembly Defects” .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

R.P. Dator, R. L. Rohlfs, P.A. Limbach (10-2009. ) (In Press. ) Isolation and Characterization of Ribosome Assembly Intermediates .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

T. D. Hilson, P.A. Limbach (10-2009. ) (In Press. ) Developing and Optimizing and a Mass Spec-Friendly Protocol for Fractionation of tRNAs Through Two-Dimensional Polyacrylamide Gel Electrophoresis .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

Catron, Brittany; Afton, Scott; Limbach, Patrick; Caruso, Joseph A. (01-2010. ) (In Press. ) Utilizing capillary HPLC-ICPMS for identification of oligonucleotide:peptide heteroconjugates .Winter Conference on Plasma Spectrochemistry, Fort Meyers, Florida. . Conference. . Level:International

Balasubrahmanyam Addepalli, Patrick Limbach (01-2010. ) (In Press. ) Mass Spectrometry based detection of pseudouridine in RNA .22nd Sanibel Conference on Mass Spectrometry: From Structural Biology to Drug Discovery: New Roles for Mass Spectrometry of Nucleic Acids, St. Pete Beach, FL. . Conference. . Level:International

Rebecca L. Rohlfs, Romel Dator, Patrick. A. Limbach (01-2010. ) (In Press. ) “Ribosome Modifications and Assembly Defects” .22nd Sanibel Conference on Mass Spectrometry: From Structural Biology to Drug Discovery: New Roles for Mass Spectrometry of Nucleic Acids, St. Pete Beach, FL. . Conference. . Level:International

Susan P Russell, Patrick A. Limbach, Brian P. Anton, Elisabeth A. Raleigh and Richard Roberts (01-2010. ) (In Press. ) Characterization of the YmcB and YqeV tRNA Methylthiotransferases of Bacillus subtilis .22nd Sanibel Conference on Mass Spectrometry: From Structural Biology to Drug Discovery: New Roles for Mass Spectrometry of Nucleic Acids, St. Pete Beach, FL. . Conference. . Level:International

Catron, Brittany; Caruso, Joseph; Giliberti, Jacqueline; Janssen, Gary; Limbach, Patrick A. (04-2010. ) (In Press. ) Detection of Peptide-Oligonucleotide Heteroconjugates and Protein:RNA Cross-links via Capillary ICPMS .Seventh Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH. . Conference. . Level:Regional

Romel Dator, Patrick Limbach (04-2010. ) (In Press. ) Mass Spectrometry-based Characterization of Antibiotic-Induced Ribosome Assembly Intermediates .Seventh Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH. . Conference. . Level:Regional

Catron, Brittany; Caruso, Joseph; Giliberti, Jacqueline; Janssen, Gary; Limbach, Patrick A. (05-2010. ) (In Press. ) Detection of Peptide-Oligonucleotide Heteroconjugates and Protein:RNA Cross-links via Capillary ICPMS .58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, UT. . Conference. . Level:National

Siwei Li, Colette Castleberry, Patrick A. Limbach (05-2010. ) (In Press. ) Monitoring Changes in the Expression Levels of Transfer RNAs by LC-MS .58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, UT. . Conference. . Level:National

Balasubrahmanyam Addepalli, Patrick Limbach (06-2010. ) (In Press. ) Quantification of pseudouridine in RNA .15th Annual Meeting of the RNA Society, Seattle, WA. . Professional Meeting. . Level:International

Romel Dator, Patrick Limbach (06-2010. ) (In Press. ) Mass Spectrometry-based Characterization of Ribosome Assembly Intermediates .15th Annual Meeting of the RNA Society, Seattle, WA. . Professional Meeting. . Level:International

Rebecca L. Rohlfs, Romel Dator, Rachel Helpling, Patrick A. Limbach (06-2010. ) (In Press. ) “Ribosome Modifications and Assembly Defects” .15th Annual Meeting of the RNA Society, Seattle, WA. . Professional Meeting. . Level:International

Susan P. Russell and Patrick A. Limbach (06-2010. ) (In Press. ) Measuring Global Changes in Post-transcriptionally Modified Nucleosides Induced by Heat Shock .15th Annual Meeting of the RNA Society, Seattle, WA. . Professional Meeting. . Level:International

M. Zorc, K. Krivos, C. Wetzel, P.A. Limbach (09-2010. ) (In Press. ) Elimination of Ribonuclease Contamination in Transfer RNA Sequencing .2010 University of Cincinnati Women in Science and Engineering Program, Cincinnati, OH. . UC. . Level:University

Romel Dator, Patrick Limbach (10-2010. ) (In Press. ) Characterization of Improperly Folded Ribosome Assembly Intermediates from Ribonuclease E Mutants .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

Siwei Li, Colette Castleberry, Patrick A. Limbach (10-2010. ) (In Press. ) Monitoring Changes in the Expression Levels of Transfer RNAs by LC-MS .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

Collin Wetzel, Patrick. A. Limbach (10-2010. ) (In Press. ) HPLC vs. UPLC tandem mass spectrometry for the detection of transfer ribonucleic acid (tRNA) signature digestion products .Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH. . Professional Meeting. . Level:Local

Romel Dator, Patrick Limbach (10-2010. ) (In Press. ) Characterization of Improperly Folded Ribosome Assembly Intermediates from Ribonuclease E Mutants .Rustbelt RNA Meeting, Cleveland, OH. . Conference. . Level:Regional

Siwei Li, Patrick A. Limbach (10-2010. ) (In Press. ) Comparative Identification of Sequence Variation in tRNAs from Two Organisms by LC-MS” .Rustbelt RNA Meeting, Cleveland, OH. . Conference. . Level:Regional

Collin Wetzel, Patrick. A. Limbach (10-2010. ) (In Press. ) HPLC vs. UPLC tandem mass spectrometry for the detection of transfer ribonucleic acid (tRNA) signature digestion products .Rustbelt RNA Meeting, Cleveland, OH. . Conference. . Level:Regional

Balasubrahmanyam Addepalli, Patrick Limbach, (01-2011. ) Quantification of Pseudouridine in RNA .RNA Editing: Editing and Modification of RNA and DNA (Gordon-Kenan Research Seminar), Galveston Island Texas. . Level:International

Brittany Catron, Joseph Caruso, Jacqueline Giliberti, Gary Janssen, Patrick Limbach (01-2011. ) Detection of Peptide-Oligonucleotide Heteroconjugates and Protein:RNA Cross-links via Capillary ICPMS .2011 Winter Conference on Plasma Spectrochemistry, Zarazoga, Spain. . Level:International

Romel Dator, Patrick Limbach (04-2011. ) Mass Spectrometry-based Characterization of Antibiotic-Induced Ribosome Assembly Intermediates .8th Annual Ohio Mass Spectrometry Symposium, Columbus, OH. . Level:State

Siwei Li, Patrick A. Limbach (04-2011. ) A New Method for Sequencing Ribonucleic Acids (RNAs) Using Isotope Labeling and LC-MS/MS .8th Annual Ohio Mass Spectrometry Symposium, Columbus, OH. . Level:State

Collin Wetzel, Patrick. A. Limbach (04-2011. ) HPLC vs. UPLC tandem mass spectrometry for the detection of transferribonucleic acid (tRNA) signature digestion products .8th Annual Ohio Mass Spectrometry Symposium, Columbus, OH. . Level:State

Brittany Catron, Joseph Caruso, Jacqueline Giliberti, Gary Janssen, Patrick Limbach (06-2011. ) Analysis of Ribosomal Protein S1 and Messenger Ribonucleic Acid (mRNA) Cross-links by ICPMS and LC-MS/MS .59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO. . Level:International

Balasubrahmanyam Addepalli, Patrick Limbach (06-2011. ) Pseudouridine Quantification in Transfer and Ribosomal RNAs by Selected Reaction Monitoring Mass Spectrometry .59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO. . Level:International

Siwei Li, Patrick A. Limbach (06-2011. ) A New Method for Sequencing Ribonucleic Acids (RNAs) Using Isotope Labeling and LC-MS/MS .59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO. . Level:International

Rebecca L. Rohlfs, Patrick A. Limbach (06-2011. ) Site-Specific Quantification of Ribosomal RNA Modification Profiles by Mass Spectrometry .59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO. . Level:International

Romel Dator, Patrick Limbach (06-2011. ) Using Mass Spectrometry to Identify the Components of the Erythromycin-Induced 50S Ribosomal Subunit Assembly Particle in Escherichia coli .59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO. . Level:International

Lihua Fu, Patrick A. Limbach (10-2011. ) Identification of tRNA modifications in Thermus thermophilus HB8 wild type and ΔTTHA1897 mutant strains .2011 Rustbelt RNA Meeting, Dayton, OH. . Level:Regional

Collin Wetzel, Patrick. A. Limbach (10-2011. ) Global identification of tRNA through the targeted detection of unique transitions .2011 Rustbelt RNA Meeting, Dayton, OH. . Level:Regional

Kirk W. Gaston, Gabriele Phillips, Valerie De Crecy-Lagard, and Patrick A. Limbach (10-2011. ) Archaeosine Synthesis Pathways and Production in E. coli tRNAs .2011 Rustbelt RNA Meeting, Dayton, OH. . Level:Regional

Siwei Li, Patrick A. Limbach (10-2011. ) A New Method for Sequencing Ribonucleic Acids (RNAs) Using Isotope Labeling and LC-MS/MS .2011 Rustbelt RNA Meeting, Dayton, OH. . Level:Regional

Romel Dator, Patrick Limbach (10-2011. ) Characterizing ribosome biogenesis and assembly using mass spectrometry .2011 Rustbelt RNA Meeting, Dayton, OH. . Level:Regional

Lihua Fu, Patrick A. Limbach (10-2011. ) Identification of tRNA modifications in Thermus thermophilus HB8 wild type and ΔTTHA1897 mutant strains .Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH. . Level:Local

Brittany Catron, Joseph Caruso, Jacqueline Giliberti, Gary Janssen, Patrick Limbach (10-2011. ) Analysis of Ribosomal Protein S1 and Messenger Ribonucleic Acid (mRNA) Cross-links by ICPMS and LC-MS/MS .Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH. . Level:Local

Collin Wetzel, Patrick. A. Limbach (10-2011. ) Global identification of tRNA through the targeted detection of unique transitions .Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH. . Level:Local

Romel Dator, Patrick Limbach (10-2011. ) Characterizing ribosome biogenesis and assembly using mass spectrometry .Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH. . Level:Local

Rebecca L. Rohlfs, Patrick A. Limbach (01-2011. ) Site-Specific Quantification of Ribosomal RNA Modification Profiles by Mass Spectrometry .Gordon-Kenan Research Seminar on Editing and Modification of RNA and DNA, Galveston Island Texas. . Level:International

Romel P. Dator and Patrick A. Limbach (03-2012. ) Characterizing Ribosome Biogenesis and Assembly Using Mass Spectrometry .UC Graduate Poster Forum, University of Cincinnati. . UC. .

Siwei Li and Patrick A. Limbach (05-2012. ) Identification of tRNA Sequence Isomer by Isotope Labeling and LC-MS/MS .60th ASMS conference on Mass Spectrometry and Allied Topics, Vancouver, Canada. . Professional Meeting. .

Collin Wetzel and Patrick A. Limbach (05-2012. ) Targeted Tandem Mass Spectrometry for tRNA Identification .60th ASMS conference on Mass Spectrometry and Allied Topics, Vancouver, Canada. . Professional Meeting. .

Romel P. Dator and Patrick A. Limbach (05-2012. ) Understanding Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes .17th Annual Meeting of the RNA Society, Ann Arbor, MI. . Professional Meeting. .

S. Y. Wong, B. Javid, B. Addepalli, G. Piszczek, M. B. Strader, P. A. Limbach, C. Barry (06-2012. ) Molecular mechanism of streptomycin resistance in Mycobacterium tuberculosis due to mutations in GidB, a 16S rRNA methyltransferase .American Society for Microbiology General Meeting, San Francisco, CA. . Professional Meeting. .

Romel P. Dator and Patrick A. Limbach (09-2012. ) Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes .11th European Conference on Computational Biology, Basel, Switzerland. . Conference. .

B. Addepalli, K. Eileen, S. Gregory, P.A. Limbach (10-2012. ) Comparison of transfer RNA nucleosides from Deinococcus radiodurans and D. saxicola .Rustbelt RNA Meeting, Dayton, OH. . Conference. .

Kirk W. Gaston and Patrick A. Limbach (10-2012. ) Photo-activated Oxidation of tRNA with Riboflavin .Rustbelt RNA Meeting, Dayton, OH. . Conference. .

Siwei Li and Patrick A. Limbach (10-2012. ) Automated Analysis of LC-MS data from Isotopically Labeled RNA .Rustbelt RNA Meeting, Dayton, OH. . Conference. .

Romel P. Dator and Patrick A. Limbach (10-2012. ) Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes .Rustbelt RNA Meeting, Dayton, OH. . Conference. .

Romel P. Dator and Patrick A. Limbach (10-2012. ) Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes .Ralph and Helen Oesper Symposium, Cincinnati, OH. . Professional Meeting. .

Carolin A. Aldinger, Anne-Katrin Leisinger, Kirk W. Gaston, Patrick A. Limbach, Gabor L. Igloi (12-2012. ) The absence of A-to-I editing in the anticodon of plant cytoplasmic tRNAArgACG demands a relaxation of the wobble rules in plants .XXIV tRNA Conference, Olmué, Chile. . Conference. .

John J. Perona, Annia Rodriguez-Hernandez, Jessica Spears, Kirk W. Gaston, Patrick A. Limbach, Howard Gamper, Ya-Ming Hou, Paul Agris (12-2012. ) Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position .XXIV tRNA Conference, Olmué, Chile. . Conference. .

Robert Ross, Kirk W. Gaston, Balu Addepalli and Patrick A. Limbach (10-2012. ) Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34 .Rustbelt RNA Meeting, Dayton, OH. . Conference. .

Robert Ross, Kirk W. Gaston, Balu Addepalli and Patrick A. Limbach (10-2012. ) Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34 .Ralph and Helen Oesper Symposium, University of Cincinnati. . Professional Meeting. .

Wunan Shi, Collin Wetzel and Patrick A. Limbach (03-2012. ) Total tRNA Mapping in Thermus thermophulis Using LC-MS/MS .UC Graduate Poster Forum, University of Cincinnati. . UC. .

Wunan Shi, Collin Wetzel and Patrick A. Limbach (10-2012. ) High throughput De Novo Sequencing of Total tRNAs in Thermus thermophulis Using LC-MS/MS .Ralph and Helen Oesper Symposium, University of Cincinnati. . Professional Meeting. .

Wunan Shi, Collin Wetzel and Patrick A. Limbach (10-2012. ) High throughput De Novo Sequencing of Total tRNAs in Thermus thermophulis Using LC-MS/MS .Rustbelt RNA Meeting, Dayton, OH. . Conference. .

Siwei Li and Patrick A. Limbach (10-2012. ) Automated Analysis of LC-MS data from Isotopically Labeled RNA .Ralph and Helen Oesper Symposium, University of Cincinnati. . Professional Meeting. .

Kirk W. Gaston and Patrick A. Limbach (01-2013. ) Photo-activated Oxidation of tRNA with Riboflavin .Gordon Research Conference: RNA Editing and Modification, Galveston Island, TX. . Conference. .

Siwei Li and Patrick A. Limbach (04-2013. ) Automated Comparative Analysis of LC-MS datasets from Isotopically Labeled RNA .2013 International Conference of RNA Nanotechnology and Therapeutics, Lexington, KY. . Conference. .

Collin Wetzel and Patrick. A. Limbach (04-2013. ) Targeted Tandem Mass Spectrometry for tRNA Identification .2013 International Conference of RNA Nanotechnology and Therapeutics, Lexington, KY. . Conference. .

S. Kesanam, B. Addepalli, P. A. Limbach (04-2013. ) Overexpression and Purification of the RNA-specific hydrolytic enzyme RNase U2 .University of Cincinnati Undergraduate Research Conference, Cincinnati OH. . UC. .

B. Addepalli and P. A. Limbach (06-2013. ) Method for pseudouridine detection and quantification in RNA by chemical derivatization and tandem mass spectrometry .61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN. . Conference. .

Romel P. Dator, Kirk W. Gaston, Patrick A. Limbach (06-2013. ) Quantitation of Bacterial Ribosomal Proteins by Mass Spectrometry .61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN. . Conference. .

Kirk W. Gaston and Patrick A. Limbach (06-2013. ) Mass spectral analysis of modified nucleosides and nucleoside monophosphates .61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN. . Conference. .

Siwei Li and Patrick A. Limbach (06-2013. ) High Throughput Analysis of LC-MS datasets from Isotopically Labeled RNA .61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN. . Conference. .

Robert Ross and Patrick A. Limbach (06-2013. ) Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34 .61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN. . Conference. .

Romel P. Dator, Kirk W. Gaston, Patrick A. Limbach (07-2013. ) Characterizing Ribosomes and Ribosome Assembly Complexes by Mass Spectrometry .Ribosomes Conference, Napa Valley, CA,. . Conference. .

T. Chen, B. Addepalli, N. Shkriabai, M. Kvaratskhelia, P. A. Limbach, V. Gopalan (10-2013. ) Mass spectrometry-aided identification of the contact sites between a precursor tRNA and a proteinaceous RNase P from Arabidopsis thaliana .Finger Lakes RNA Conference, Canandaigua NY. . Conference. .

Xiaoyu Cao, Patrick A. Limbach (10-2013. ) The Posttranscriptionally Modified Nucleosides of Streptomyces griseus tRNAs .Ralph and Helen Oesper Symposium, Cincinnati, OH. . Professional Meeting. .

Y. Jiao, P. A. Limbach (10-2013. ) Ligating Nicotinamide Mononucleotide to Transfer RNA Digestion Products by T4 RNA Ligase .Ralph and Helen Oesper Symposium, Cincinnati, OH. . Professional Meeting. .

Whitney Houser and Patrick A. Limbach (10-2013. ) LC-MS/MS analysis of tRNA using digestion with RNase U2 and Lambda Protein Phosphatase .Ralph and Helen Oesper Symposium, Cincinnati, OH. . Professional Meeting. .

Katherine M. Anderson, Ian M.C. Fleming, Kirk W. Gaston, Pat A. Limbach, Mary Anne Rubio, Juan D. Alfonzo (10-2013. ) Functional dependence of a tRNA editing deaminase and a methyltransferase in Trypanosoma brucei .Rustbelt RNA Meeting, Cleveland, OH. . Conference. .

Xiaoyu Cao, Patrick A. Limbach (10-2013. ) The Posttranscriptionally Modified Nucleosides of Streptomyces griseus tRNAs .Rustbelt RNA Meeting, Cleveland, OH. . Conference. .

T. Chen, B. Addepalli, N. Shkriabai, M. Kvaratskhelia, P. A. Limbach, V. Gopalan (10-2013. ) Mass spectrometry-aided identification of the contact sites between a precursor tRNA and a proteinaceous RNase P from Arabidopsis thaliana .Rustbelt RNA Meeting, Cleveland, OH. . Conference. .

Kirk W. Gaston, Caroline Köhrer, Debabrata Mandal, Henri Grosjean, Patrick A. Limbach, Uttam L. RajBhandary (10-2013. ) Life without tRNA Ile-lysidine synthetase: Translation of the isoleucine codon AUA without the canonical tRNA Ile .Rustbelt RNA Meeting, Cleveland, OH. . Conference. .

Paul J. Sample, Ludek Koreny, Zdenek Paris, Kirk W. Gaston, Pat Limbach, Juan D. Alfonzo (10-2013. ) A common modification in an unusual place: Wybutosine within the Trypanosome mitochondrion .Rustbelt RNA Meeting, Cleveland, OH. . Conference. .

Collin Wetzel, Patrick. A. Limbach (10-2013. ) Improving Comparative Analysis of tRNA by Mass Spectrometry Using Isotopic enrichments .Rustbelt RNA Meeting, Cleveland, OH. . Conference. .

Robert Ross, Drew Sauter and Patrick A Limbach (03-2014. ) Inductive Based Fluidics Mass Spectrometry for the Analysis of RNAs Containing Modified Nucleosides .Graduate School Research Forum, University of Cincinnati. .

Drew Sauter, Andrew D Sauter III, Gary Gronenewold, Roy Helmy, Robert Ross, and Patrick Limbach (04-2014. ) Droplets + Electric Fields → Intense ESI and MALDI Mass Spectra .The Pittsburgh Conference, Chicago, IL. .

Robert Ross, Drew Sauter and Patrick A Limbach (05-2014. ) Inductive Based Fluidics Mass Spectrometry for the Analysis of RNAs Containing Modified Nucleosides .Ohio Mass Spectrometry Symposium, Ohio State University. .

Xiaoyu Cao, Patrick A. Limbach (05-2014. ) An exclusion list strategy to improve detection of modified oligonucleotides from tRNA .Ohio Mass Spectrometry Symposium, Ohio State University. .

Yang Jiao, Patrick A. Limbach (05-2014. ) A labeling method for multiplex comparative analysis of tRNA samples with T4 RNA ligase .Ohio Mass Spectrometry Symposium, Ohio State University. .

Whitney M. Houser, Patrick A. Limbach (05-2014. ) Improving Mass Spectral Analysis of Oligoribonucleotides Obtained from Ribonuclease U2 Digests .Ohio Mass Spectrometry Symposium, Ohio State University. .

Kirk Gaston, Patrick Limbach (06-2014. ) Alternative Ammonium Acetate Mobile Phases for LC-MS/MS Analysis of Oligonucleotides .61st ASMS conference on Mass Spectrometry and Allied Topics, Baltimore, MD. .

Robert Ross, Drew Sauter and Patrick A Limbach (06-2014. ) Inductive Based Fluidics Mass Spectrometry for the Analysis of RNAs Containing Modified Nucleosides .61st ASMS conference on Mass Spectrometry and Allied Topics, Baltimore, MD. .

Xiaoyu Cao, Patrick A. Limbach (06-2014. ) An exclusion list strategy to improve detection of modified oligonucleotides from tRNA .61st ASMS conference on Mass Spectrometry and Allied Topics, Baltimore, MD. .

Whitney M. Houser, Patrick A. Limbach (06-2014. ) Improving Mass Spectral Analysis of Oligoribonucleotides Obtained from Ribonuclease U2 Digests .61st ASMS conference on Mass Spectrometry and Allied Topics, Baltimore, MD. .

Nicholas P. Lesner, Patrick A. Limbach, and Balasubrahmanyam Addepalli (08-2014. ) Optimal conditions for expression and purification of recombinant U-specific MC1 ribonuclease .Summer REU Poster Session, University of Cincinnati. .

Robert Ross, Drew Sauter and Patrick A Limbach (10-2014. ) Inductive Based Fluidics Mass Spectrometry for the Analysis of RNAs Containing Modified Nucleosides .Rustbelt RNA Meeting, Pittsburgh, PA. .

Xiaoyu Cao, Patrick A. Limbach (10-2014. ) Discovery-based Ribonuclease (RNase) mapping with mass exclusion list locating tRNA modifications .Rustbelt RNA Meeting, Pittsburgh, PA. .

Whitney M Houser, Annika B Kotter, Balasubrahmanyam Addepalli and Patrick A Limbach (10-2014. ) Enzymatic generation of 3’-phosphate from 2’,3’-cyclic phosphate endonuclease digestion products of recombinant RNase U2 for improved LC/MS analysis of RNAs .Rustbelt RNA Meeting, Pittsburgh, PA. .

Sarah Venus, Patrick A Limbach and Balasubrahmanyam Addepalli (10-2014. ) Purification and characterization of cytidine-specific ribonuclease, Cusativin, for mapping nucleoside modifications in RNA .Rustbelt RNA Meeting, Pittsburgh, PA. .

Beulah M Solivio, Balasubrahmanyam Addepalli and Patrick A Limbach (10-2014. ) Mutagenesis of RNase U2 for Enhanced Specificity .Rustbelt RNA Meeting, Pittsburgh, PA. .

Xiaoyu Cao, Patrick A. Limbach (10-2014. ) Discovery-based Ribonuclease (RNase) mapping with mass exclusion list locating tRNA modifications .Ralph and Helen Oesper Symposium, University of Cincinnati. .

Yang Jiao, Patrick A. Limbach (10-2014. ) Ligating Nicotinamide Mononucleotide to Tansfer RNA Digestion product by T4 RNA Ligase .Ralph and Helen Oesper Symposium, University of Cincinnati. .

Whitney M Houser, Annika B Kotter, Balasubrahmanyam Addepalli and Patrick A Limbach (10-2014. ) Enzymatic generation of 3’-phosphate from 2’,3’-cyclic phosphate endonuclease digestion products of recombinant RNase U2 for improved LC/MS analysis of RNAs .Ralph and Helen Oesper Symposium, University of Cincinnati. .

Mellie June Paulines, Robert Ross and Patrick A. Limbach (10-2014. ) Identifying the modified guanosine at position 34 of placental human tRNA Asp(GUC) and tRNA Asn(GUU) .Ralph and Helen Oesper Symposium, University of Cincinnati. .

Beulah M Solivio, Balasubrahmanyam Addepalli and Patrick A Limbach (10-2014. ) Mutagenesis of RNase U2 for Enhanced Specificity .Ralph and Helen Oesper Symposium, University of Cincinnati. .

Sarah Venus, Patrick A Limbach and Balasubrahmanyam Addepalli (10-2014. ) Purification and characterization of cytidine-specific ribonuclease, Cusativin, for mapping nucleoside modifications in RNA .Ralph and Helen Oesper Symposium, University of Cincinnati. .

Balasubrahmanyam Addepalli, Sarah Venus, Nicholas P. Lesner, and Patrick A. Limbach (03-2015. ) Development of novel ribonucleases for sequencing chemical modifications in RNA .RNA Biology 2015, Natcher Conference Center, NIH, Bethesda, MD. .

Robert Ross, Drew Sauter and Patrick A Limbach (03-2015. ) Optimized Conditions for the Analysis of Oligonucleotides by Induction Based Fluidics Mass Spectrometry .Gordon Research Conference on RNA Editing and Modification, Lucca (Barga), Italy. .

Xiaoyu Cao and Patrick A. Limbach (03-2015. ) A Discovery-based Strategy for RNA Modification Mapping by LC-MS/MS .Gordon Research Conference on RNA Editing and Modification, Lucca (Barga), Italy. .

Beulah Mae Ann Solivio, Balasubramanyam Addepalli, and Patrick A. Limbach (05-2015. ) Improving RNA Modification Mapping by Mutagenesis of RNase U2 .12th Annual Ohio Valley Mass Spectrometry Symposium, Columbus OH. .

Ningxi Yu and Patrick A. Limbach (05-2015. ) Development and validation of a tandem mass spectral library for modified oligonucleotides from transfer RNAs .12th Annual Ohio Valley Mass Spectrometry Symposium, Columbus OH. .

Mellie June Paulines, Robert Ross and Patrick A. Limbach (03-2015. ) Characterizing the Post-transcriptional Modifications in Human Transfer Ribonucleic Acids by Mass Spectrometry .Graduate Student Expo and Poster Forum, Cincinnati, OH. .

Whitney M. Houser, Annika Butterer, Balasubrahmanym Addepalli, Patrick A. Limbach (05-2015. ) Combining recombinant ribonuclease U2 and protein phosphatase for RNA modification mapping by liquid chromatography–mass spectrometry .12th Annual Ohio Valley Mass Spectrometry Symposium, Columbus OH. .

Mellie June Paulines, Patrick Limbach (05-2015. ) Modified Guanosine at position 34 of placental human tRNA Asp(GUC) and tRNA Asn(GUU) .12th Annual Ohio Valley Mass Spectrometry Symposium, Columbus OH. .

Beulah Mae Ann Solivio, Balasubramanyam Addepalli, and Patrick A. Limbach (06-2015. ) Improving RNA Modification Mapping by Mutagenesis of RNase U2 .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Whitney M. Houser, Lauren Schultz, Patrick A. Limbach (06-2015. ) “LC-MS/MS Characterization of Cladosporium sphaerospermum RNA Modifications .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Robert Ross, Drew Sauter and Patrick A Limbach (06-2015. ) Induction Based Fluidics (IBF) for Mass Spectrometric Analysis of Oligonucleotides .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Balasubrahmanyam Addepalli, Sarah Venus, Nicholas P. Lesner, and Patrick A. Limbach (06-2015. ) Mapping Chemical Modifications in RNA by Employing C-specific and U-specific Ribonucleases and LC-MS .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Ningxi Yu and Patrick A. Limbach (06-2015. ) Development and validation of post-transcriptional modified tRNA tandem mass spectral library .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Xiaoyu Cao and Patrick A. Limbach (06-2015. ) The Efficiency of genome-based mass exclusion list exploring unknown modifications and reducing redundant data collection .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Collin Wetzel and Patrick A. Limbach (06-2015. ) A Proteomics-like Pipeline for the Epitranscriptome: Automated Analysis of Posttranscriptionally Modified RNAs .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Mellie June Paulines and Patrick A Limbach (06-2015. ) Modified Guanosine at position 34 of placental human tRNA Asp(GUC) and tRNA Asn(GUU) .63rd ASMS conference on Mass Spectrometry and Allied Topics, St. Louis MO. .

Whitney M. Houser, Lauren Schultz, Patrick A. Limbach (07-2015. ) LC-MS/MS Characterization of Cladosporium sphaerospermum RNA Modifications .Defense Threat Reduction Agency Basic Research Technical Review, Springfield, VA. .

Adaeze Aninweze, Patrick A. Limbach and Balasubrahmanyam Addepalli (08-2015. ) Quantification of Pseudouridine modifications through uridine specific cleavage of RNA .Chemistry REU Program, Cincinnati, OH. .

Beulah Mae Ann V. Solivio, Balasubramanyam Addepalli and Patrick Limbach (10-2015. ) A semi-rational approach to probe the importance of RNase U2 binding site amino acids .Rustbelt RNA Meeting, Huron OH. .

Whitney M. Houser, Lauren Schultz, Patrick A. Limbach (10-2015. ) LC-MS/MS Characterization of Cladosporium sphaerospermum RNA Modifications .Rustbelt RNA Meeting, Huron OH. .

Mellie June Paulines and Patrick A Limbach (10-2015. ) Comparative Analysis of Transfer RNAs by Mass Spectrometry .Rustbelt RNA Meeting, Huron OH. .

Alan Kessler, Zdenek Paris, Mellie June Paulines, Raphael Lopes, Patrick A. Limbach, and Juan D. Alfonzo (10-2015. ) A putative tRNA retrograde transport system in Trypanosoma brucei necessary for the tRNA modification queuosine .Rustbelt RNA Meeting, Huron OH. .

Beulah Mae Ann V. Solivio, Balasubramanyam Addepalli and Patrick Limbach (11-2015. ) A semi-rational approach to probe the importance of RNase U2 binding site amino acids .Ralph and Helen Oesper Symposium, Cincinnati, OH. .

Whitney M. Houser, Lauren Schultz, Patrick A. Limbach (11-2015. ) LC-MS/MS Characterization of Cladosporium sphaerospermum RNA Modifications .Ralph and Helen Oesper Symposium, Cincinnati, OH. .

Congliang Sun, Zalfa Abdel-Malek, Patrick A Limbach and Balasubrahmanyam Addepalli (11-2015. ) UV-induced effects on RNA and RNA-modifications .Ralph and Helen Oesper Symposium, Cincinnati, OH. .

Kayla M. Borland and Patrick A. Limbach (11-2015. ) Investigating the use of polyA polymerase (PAP) with modified NTP labeling for multiplex analysis of tRNA digestion products .Ralph and Helen Oesper Symposium, Cincinnati, OH. .

Ningxi Yu and Patrick A. Limbach (11-2015. ) Ribonucleic acids modification mapping mapping method development .Ralph and Helen Oesper Symposium, Cincinnati, OH. .

Mellie June Paulines and Patrick A Limbach (11-2015. ) Comparative Analysis of Transfer RNAs by Mass Spectrometry .Ralph and Helen Oesper Symposium, Cincinnati, OH. .

Balasubrahmanyam Addepalli, Sarah Venus, Nicholas P. Lesner, and Patrick A. Limbach (11-2015. ) Novel ribonucleases for sequencing chemical modifications in RNA .Ohio Valley for Life Sciences (OVALS) Conference, Louisville, KY. .

Congliang Sun, Zalfa Abdel-Malek, Patrick A Limbach and Balasubrahmanyam Addepalli (11-2015. ) UV-induced effects on RNA and RNA-modifications .Cancer Biology & Hematology/Oncology Joint Retreat, Cincinnati, OH. .

Mellie June Paulines and Patrick A. Limbach (02-2016. ) Differential mass spectrometry analysis of transfer RNA by stable isotope labelling .Graduate Student Expo and Poster Forum, Cincinnati, OH. .

Vanessa Short, Priti Thakur, Patrick Limbach and Balasubrahmanyam Addepalli (04-2016. ) Optimization of cytidine-specific cleavage mediated by recombinant cusativin for detecting modifications in RNA .URSC conference, Cincinnati, OH. .

Robert L Ross, Andrew D. Sauter, Drew Sauter and Patrick A Limbach (04-2016. ) Inductive Electric Fields Applied to ESI, MALDI & Sample Handling .Sanibel ASMS Conference, Sanibel, FL. .

Priti Thakur, Vanessa Short, Patrick Limbach and Balasubrahmanyam Addepalli (05-2016. ) Optimization of cytidine-specific cleavage mediated by recombinant cusativin for detecting modifications in RNA .ACS 47th Central Regional Meeting, Covington, KY. .

Congliang Sun, Zalfa Abdel-Malek, Patrick A Limbach and Balasubrahmanyam Addepalli (05-2016. ) Probing UV-induced effects on RNA and RNA-modifications .ACS 47th Central Regional Meeting, Covington, KY. .

Balasubrahmanyam Addepalli and Patrick A Limbach (05-2016. ) Novel Ribonucleases for Sequencing Chemical Modifications in RNA .ACS 47th Central Regional Meeting, Covington, KY. .

Mellie June Paulines and Patrick A. Limbach (05-2016. ) Differential mass spectrometry analysis of transfer RNA by stable isotope labelling .ACS 47th Central Regional Meeting, Covington, KY. .

Priti Thakur, Vanessa Short, Patrick Limbach and Balasubrahmanyam Addepalli (05-2016. ) Optimization of cytidine-specific cleavage mediated by recombinant cusativin for detecting modifications in RNA .13th Annual Ohio Mass Spectrometry Symposium, Columbus OH. .

Xiaoyu Cao and Patrick A Limbach (06-2016. ) RNA Modification Mapping Using a Universal Exclusion List by LC-MS/MS .64th American Association for Mass Spectrometry Annual Conference, San Antonio, TX. .

Ningxi Yu and Patrick A Limbach (06-2016. ) RNAModMapper - RNA Modification Mapping Software Development .64th American Association for Mass Spectrometry Annual Conference, San Antonio, TX. .

Mellie June Paulines and Patrick A. Limbach (06-2016. ) Differential mass spectrometry analysis of transfer RNA by stable isotope labelling .64th American Association for Mass Spectrometry Annual Conference, San Antonio, TX. .

Congliang Sun, Zalfa Abdel-Malek, Kazumasa Wakamatsu, Patrick A Limbach and Balasubrahmanyam Addepalli (06-2016. ) UV-induced effects on RNA and RNA-modifications .64th American Association for Mass Spectrometry Annual Conference, San Antonio, TX. .

Congliang Sun, Zalfa Abdel-Malek, Kazumasa Wakamatsu, Patrick A Limbach and Balasubrahmanyam Addepalli (06-2016. ) Probing UV-induced effects on RNA and RNA-modifications by LC-MS .64th American Association for Mass Spectrometry Annual Conference, San Antonio, TX. .

Kayla Borland and Patrick A. Limbach (06-2016. ) Using polyA polymerase (PAP) with azido modified NTP labeling for multiplex analysis of tRNA digest products .64th American Association for Mass Spectrometry Annual Conference, San Antonio, TX. .

Beulah Mae Ann Solivio, Balasubrahmanyam Addepalli, and Patrick Limbach (06-2016. ) Evaluating the Specificity of RNase U2 Variants by Mass .64th American Association for Mass Spectrometry Annual Conference, San Antonio, TX. .

Mellie June Paulines, Danielle Marler, Zach Tasset and Patrick A. Limbach (07-2016. ) Mass spectrometry based analysis of guanosine at position 34 of human placenta tRNA Asp (GUC), Asn (GUU) and His (GUG) .Gordon Research Conference for Post-Transcriptional Gene Regulation, Stowe, VT. .

Mellie June Paulines and Patrick A. Limbach (10-2016. ) Differential mass spectrometry analysis of transfer RNA by stable isotope labelling .Rustbelt RNA Meeting, Cleveland, OH. .

Alan Kessler, Zdenek Paris, Mellie June Paulines, Raphael Lopes, Patrick A. Limbach, and Juan D. Alfonzo (10-2016. ) A putative tRNA retrograde transport system in Trypanosoma brucei necessary for the tRNA modification queuosine .Rustbelt RNA Meeting, Cleveland, OH. .

Ruoxia Zhao, Bala Addepalli and Patrick A. Limbach (10-2016. ) Detection of Post-Transcriptional RNA Modifications in the Radioresistant Bacterium Deinococcus radiodurans R1 .Rustbelt RNA Meeting, Cleveland, OH. .

Kayla Borland and Patrick A. Limbach (10-2016. ) Azido Modified NTP as chemical handles for multiplex analysis of tRNA digestion products .Rustbelt RNA Meeting, Cleveland, OH. .

Christina Psihountas, Collin Wetzel, and Patrick A Limbach (10-2016. ) Verification of methylated Inosine in Bacillus subtilis tRNA .Oesper Symposium, Cincinnati, OH. .

Ruoxia Zhao, Bala Addepalli and Patrick A. Limbach (10-2016. ) Detection of Post-Transcriptional RNA Modifications in the Radioresistant Bacterium Deinococcus radiodurans R1 .Oesper Symposium, Cincinnati, OH. .

Melissa Kelley , Mellie June Paulines, and Patrick A Limbach (10-2016. ) Determining the impact of irradiation on melanized radiotrophic fungi with varying metabolic resources .Oesper Symposium, Cincinnati, OH. .

Peter A Lobue and Patrick A Limbach (10-2016. ) High Resolution, Accurate Mass Spectrometry of Modified RNA .Oesper Symposium, Cincinnati, OH. .

Robert L Ross, Manasses Jora, Drew Sauter, Andrew Sauter III, Patrick A Limbach (10-2016. ) Droplet Based Sampling of RNA Hydrolysates by Inductive Based Fluidics .Asilomar ASMS Conference, Asilomar, CA. .

Mellie June Paulines and Patrick A. Limbach (03-2017. ) Mass spectrometry analysis of tRNAs from the pathogenic fungi Cryptococcus neoformans .Gordon Research Conference for RNA Editing, Ventura, CA. .

Balasubrahmanyam Addepalli and Patrick A Limbach (03-2017. ) Novel Ribonucleases for Sequencing Chemical Modifications in RNA .Gordon Research Conference for RNA Editing, Ventura, CA. .

Ruoxia Zhao, Bala Addepalli and Patrick A. Limbach (05-2017. ) Detection and Mapping of Post-Transcriptional tRNA Modifications in the Radioresistant Bacterium Deinococcus radiodurans R1 .Inaugural Conference on Food and Nutritional Metabolomics and 14th Annual Ohio Mass Spectrometry Symposium, Columbus OH. .

Christina Psihountas, Manasses Jora, and Patrick A Limbach (05-2017. ) Detection of tRNA modifications changes during growth phase transitions in Bacillus subtilis by LC-MS/MS .14th Annual Ohio Mass Spectrometry Symposium, Columbus OH. .

Melissa Kelley, Mellie June Paulines, and Patrick A. Limbach (05-2017. ) Determining the translational impact of varying metabolic resources on radiotrophic fungi through nucleoside and oligo analysis .14th Annual Ohio Mass Spectrometry Symposium, Columbus OH. .

Manasses Jora, Robert Ross, Drew Sauter, Andrew Sauter III and Patrick A. Limbach (05-2017. ) Induction-Based Fluidics: An Alternative Ionization Source for LC-MS/MS Analysis of RNA Nucleosides .14th Annual Ohio Mass Spectrometry Symposium, Columbus OH. .

Peter A Lobue (05-2017. ) Data-Independent Mass Spectrometry of Modified RNA .14th Annual Ohio Mass Spectrometry Symposium, Columbus OH. .

Ningxi Yu and Patrick A Limbach (06-2017. ) Identification of post-transcriptional modifications of tRNAs in archaeal organisms using liquid chromatography and mass spectrometry .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Mellie June Paulines and Patrick A. Limbach (06-2017. ) Mass spectrometry analysis of tRNAs from the pathogenic fungi Cryptococcus neoformans .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Priti Thakur, Patrick Limbach and Balasubrahmanyam Addepalli (06-2017. ) Novel cytidine specific ribonuclease for MS-based RNA modification mapping .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Congliang Sun, Patrick A Limbach and Balasubrahmanyam Addepalli (06-2017. ) LC-MS based detection of radiation induced effects on RNA and its chemical modifications .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Ruoxia Zhao, Bala Addepalli and Patrick A. Limbach (06-2017. ) Detection and Quantification of Post-Transcriptional tRNA Modifications in the Radioresistant Bacterium Deinococcus Radiodurans R1 .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Manasses Jora, Robert Ross and Patrick A. Limbach (06-2017. ) TiO2-Based Solid Phase Extraction and LC-Coupled Mass Spectrometry for the Detection of 2’-O-methylmodifications in tRNA .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Robert L Ross, Manasses Jora, Drew Sauter, Andrew Sauter III, Patrick A Limbach (06-2017. ) Droplet Based Sampling of RNA Hydrolysates by Inductive Based Fluidics .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Peter A Lobue, Balasubrahmanyam Addepalli, Patrick A Limbach (06-2017. ) RNA Modification Mapping Using Mass-to-Charge Dependent Collision Energies on a Synapt G2-S .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Beulah Mae Ann Solivio, Balasubrahmanyam Addepalli, and Patrick Limbach (06-2017. ) Evaluation of RNase U2 Variants by Mass Spectrometry for the Improvement of RNA Modification Mapping .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Kayla Borland and Patrick A. Limbach (06-2017. ) Multiplex analysis of oligonucleotides and RNA digestion products through polA polymerase (PAP) with azido-modified NTP labeling .65th ASMS Conference on Mass Spectrometry & Allied Topics, Indianapolis, IN. .

Mellie June Paulines and Patrick A. Limbach (06-2017. ) Mass spectrometry analysis of tRNAs from the pathogenic fungi Cryptococcus neoformans .Life Sciences Technical Review (DTRA),, Lawton, VA. .

Balasubrahmanyam Addepalli, Congliang Sun, and Patrick Limbach (08-2017. ) Probing stress-induced effects on RNA and Posttranscriptional Modifications by LC-MS .254th ACS National Meeting & Exposition, Washington, DC . .

Kayla Borland and Patrick Limbach (09-2017. ) Multiplex Analysis of Modified Oligoribonucleotides Through Poly A Polymerase (PAP) with Azido Modified NTP Labeling .1st Symposium on Nucleic Acid Modifications , Mainz, Germany. .

Kayla Borland and Patrick A. Limbach (10-2017. ) Multiplex Analysis of Modified Oligoribonucleotides Through Poly A Polymerase (PAP) with Azido Modified NTP Labeling .Ralph and Helen Oesper Symposium, Cincinnati, OH. .

Kelley M, Schultz L, Myers R, Limbach PA (10-2017. ) The effect of peroxide exposure on tRNA of radiotrophic fungi C. neoformans .Rusbelt RNA Meeting, Indianapolis, IN. .

Manasses Jora, Andrew Burns, Patrick A. Limbach and Balasubrahmanyam Addepalli (10-2017. ) Global Profiling of the Effects of Oxidative Stress on RNA Modifications by Liquid Chromatography-Mass Spectrometry .Rustbelt RNA Meeting, Indianapolis, IN. .

Priti Thakur, Patrick Limbach and Balasubrahmanyam Addepalli (10-2017. ) Improving the sequence coverage of RNA modification mapping by LC-MS .Rustbelt RNA Meeting, Indianapolis, IN. .

Christina Psihountas, Manasses Jora, and Patrick A. Limbach (10-2017. ) tRNA modification profile changes during growth phase transitions in Bacillus subtilis .Rustbelt RNA Meeting, Indianapolis, IN. .

Kelley M, Myers R, Jora M, Schultz L, Addepalli B, Limbach PA (11-2017. ) The effect of peroxide exposure on tRNA of the radiotrophic fungi C. neoformans .The Oesper Award Program and Symposium, Cincinnati, OH. .

Priti Thakur, Patrick Limbach and Balasubrahmanyam Addepalli (11-2017. ) Improving the sequence coverage of RNA modification mapping by LC-MS .The Oesper Award Program and Symposium, Cincinnati, OH. .

Congliang Sun, Patrick Limbach and Balasubrahmanyam Addepalli (01-2018. ) Probing stress-induced effects on RNA and post-transcriptional modifications by LC-MS .NIDA Genetics Consortium Meeting, Rockville, MD. .

Christina Psihountas, Manasses Jora, and Patrick A. Limbach (02-2018. ) tRNA modification changes during growth phase transitions in Bacillus subtilis .Graduate Student Expo, Cincinnati, OH. .

Kelley M, Myers R, Jora M, Schultz L, Addepalli B, Limbach PA (04-2018. ) The effect of peroxide exposure on the transfer RNA of radiotrophic fungi C. neoformans .Experimental Biology Conference, San Diego, CA. .

Alem, K, Myers M, Kelley M, Addepalli B, Limbach PA (04-2018. ) The impact of melanin loss on the transfer RNA of radiotrophic fungi Cladosporium sphaerospermum. Annual Undergraduate Research Symposium, Cincinnati, OH. .

Boone CC, Myers M, Kelley M, Addepalli B, Limbach PA (04-2018. ) Characterization of RNA modifications from Cladosporium sphaerospermum .Annual Undergraduate Research Symposium, Cincinnati, OH. .

Manasses Jora, Congliang Sun, Patrick A. Limbach and Balasubrahmanyam Addepalli (04-2018. ) Global Profiling of the Oxidative Stress Induced Effects on RNA Modifications by Liquid Chromatography-Tandem Mass Spectrometry .Experimental Biology Meeting, San Diego, CA. .

Priti Thakur, Patrick Limbach and Balasubrahmanyam Addepalli (04-2018. ) Improved RNA-modification mapping through employment of novel ribonucleases and LC-MS .Experimental Biology 2018, San Diego, CA. .

Manasses Jora, Andrew Burns, Robert Ross, Peter Lobue, Ruoxia Zhao, Balasubrahmanyam Addepalli and Patrick A. Limbach (05-2018. ) Differentiating Positional Isomers of Nucleoside Modifications by Higher-Energy Collisional Dissociation Mass Spectrometry .2nd Annual Ohio Mass Spectrometry and Metabolomics Symposium, Columbus OH. .

Scott Abernathy, Kayla M. Borland, and Patrick A. Limbach (05-2018. ) New Chemical Reagents for LC/MS multiplexing of Oligonucleotides .2nd Annual Ohio Mass Spectrometry and Metabolomics Symposium, Columbus OH. .

Priti Thakur, Patrick Limbach and Balasubrahmanyam Addepalli (05-2018. ) Improved RNA modification mapping through generation of overlapping digestion products .2nd Annual Ohio Mass Spectrometry and Metabolomics Symposium, Columbus OH. .

Christina Psihountas, Manasses Jora, and Patrick A. Limbach (05-2018. ) Using LC-MS/MS to detect tRNA modification profile changes during cell cycle transitions in Bacillus subtilis .2nd Annual Ohio Mass Spectrometry and Metabolomics Symposium, Columbus OH. .

Ruoxia Zhao, Robert Ross, Manasses Jora, Mellie June Paulines, Patrick Limbach (05-2018. ) An Improved Solid Phase Extraction Method for Isolating Transfer RNA .2nd Annual Ohio Mass Spectrometry and Metabolomics Symposium, Columbus OH. .

Peter A. Lobue, Bala Addepalli, Manasses Jora and Patrick A. Limbach (06-2018. ) Data-Independent Acquisition (LC-MSE) for the Quantification of RNA Oligonucleotides .66th ASMS Conference on Mass Spectrometry, San Diego, CA. .

Ningxi Yu and Patrick A. Limbach (06-2018. ) Identification of box C/D sRNA-guided 2′-O-methylation at position 34 of Sulfolobus acidocaldarius tRNAs by liquid chromatography tandem mass spectrometry .66th ASMS Conference on Mass Spectrometry, San Diego, CA. .

Mellie June Paulines, Robert Ross , Pankaj Dwivedi , Kenneth D. Greis and Patrick Limbach (06-2018. ) Unraveling the radiation resistance network of Cryptococcus neoformans by Genome Wide Transcriptome Analysis and Mass Spectrometry .66th ASMS Conference on Mass Spectrometry, San Diego, CA. .

Robert L Ross, Ningxi Yu, Mansses Jora, Ralf Tautenhahn, Patrick A. Limbach (06-2018. ) High Energy Collisional Dissociation (HCD) spectral matching for the global identification of RNA hydrolysates .66th ASMS Conference on Mass Spectrometry, San Diego, CA. .

Ruoxia Zhao, Manasses Jora, Peter Lobue, Patrick A. Limbach (06-2018. ) Detection, Quantification and Mapping of Post-Transcriptional tRNA Modifications in the Radioresistant Bacterium Deinococcus Radiodurans R1 .66th ASMS Conference on Mass Spectrometry, San Diego, CA. .

Congliang Sun, Balasubrahmanyam Addepalli, Patrick A. Limbach (06-2018. ) Mapping UVR-induced effects on Escherichia coli tRNA by LC-MS .66th ASMS Conference on Mass Spectrometry, San Diego, CA. .

Mellie June Paulines and Patrick A. Limbach (07-2018. ) Cryptococcus neoformans tRNA expression in ionizing radiation .DTRA Life Sciences Technical Review, Springfield, VA. .

Kelley M, Alem K, Myers R, Paulines MJ, Addepalli B, Limbach PA (07-2018. ) The effect of fungicide-induced melanin loss on RNA modifications in Cladosporium sphaerospermum .DTRA Life Sciences Technical Review, Springfield, VA. .

Robert L Ross, Ningxi Yu, Ralf Tautenhahn, Patrick A. Limbach (07-2018. ) High Energy Collisional Dissociation (HCD) spectral matching for the global identification of RNA hydrolysates .Gordon Research Conference on Gene Regulation, Newry, ME. .

Ruoxia Zhao, Manasses Jora, Peter Lobue, Patrick A. Limbach (07-2018. ) Detection, Quantification and Mapping of Post-Transcriptional tRNA Modifications in the Radioresistant Bacterium Deinococcus Radiodurans R1 .DTRA Life Sciences Technical Review, Springfield, VA. .

Paper Presentations

M. Hossain, P.A. Limbach (10-2007. ) Large Scale Quantitive Analysis of Non-coding RNAs (ncRNAs) by their Signature Digestion Products using Stable Isotope Labeling and MALDI-MS .Memphis, TN.

Kirk E. Lokits, Scott Afton, Karolin Kroening, Renee Kepler, Patrick A. Limbach and Joseph A. Caruso (10-2007. ) ICPMS Collision Cell: Novel Applications with Xenon Gas .Memphis, TN.

C.M. Castleberry, L. Sallans, P.A. Limbach (05-2007. ) Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS .Northern Kentucky Convention Center, Covington, KY.

M. Hossain, P.A. Limbach (05-2007. ) Relative Quantification of Large Ribosomal RNAs (rRNAs) by their Signature Digestion Products .Northern Kentucky Convention Center, Covington, KY.

L. Sallans, Y.B. Wetherill, J.K. Hess-Wilson, C.E.S. Comstock, S.A. Shah, C.R. Buncher, P.A. Limbach, S. Schwemberger, G.F. Babcock, and K.E. Knudsen (05-2007. ) Bisphenol A Facilities Bypass of Androgen Ablation Therapy in Prostrate cancer: Use of Mass Spectrometry to determine Bisphenol A Levelsin the Blood Serum of Mice .Northern Kentucky Convention Center, Covington, KY.

C.M. Castleberry, L. Sallans, P.A. Limbach (03-2007. ) Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS .Quest Conference Center, Columbus, OH .

D.-M Hamburg, M.-J. Suh and P.A. Limbach (03-2007. ) Conformational Changes and Chemical Reactivity of the Ribosome: An application of Limited Proteolysis and Matrix-assisted Laser Desorption/Ionizatization Mass Spectrometry .Quest Conference Center, Columbus, OH.

M. Hossain, P.A. Limbach (03-2007. ) Playing SUDOKU with Nucleotides .Quest Conference Center, Columbus, OH.

R.L. Rohlfs, P.A. Limbach (03-2007. ) Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotic .Quest Conference Center, Columbus, OH.

D.-M Hamburg, M.-J Suh and P.A. Limbach (02-2007. ) Mass Spectrometry of Ribosomes for structural Elucidation .Chicago, IL.

A.Durairaj, P.A. Limbach (03-2006. ) Optimizing a Mass Spectrometry-Based Approach for the Detection of CMC-derivatized Pseudouridine in RNA .Quest Conference Center, Columbus, OH.

C.M. Castleberry, L.Sallans, P. A. Limbach (03-2006. ) Developing an L.C.-MS Approach for Quantifying RNAs .Quest Conference Center, Columbus, OH.

M. Hossain, P.A. Limbach (03-2006. ) Maximizing the Detection of transfer RNAs by Multiple Ribonucleases .Quest Conference Center, Columbus, OH .

A.Piruska, I. Nikcevic, P. A. Limbach, W. R. Heineman, C. J. Seliskar (01-2006. ) Optical Detection System for Multi-Lane Plastic Microchips .Palm Springs, CA.

A.L. Dupre, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P. A. Limbach (11-2005. ) Polymer Microchips for Capillary Electrophoresis Electrospray Ionization Mass Spectrometyr (CE-ESI-MS) .Cincinnati, OH.

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (11-2005. ) Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .Cincinnati, OH.

D.-M. Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (10-2005. ) Analysis of Streptomycin Resistant and Dependent Thermus Thermophilus Ribosomal Protein S12 Mutants via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .Mt. Sterling, OH.

M. Hossain, P.A. Limbach (10-2005. ) In Search of Signature Digestion Products: tRNA Treasure Hunting .Mt. Sterling, OH.

A.Piruska, I. Nikcevic, S.H. Lee, C.H. Ahn, P.A. Limbach, W.R. Heineman, C.J. Seliskar (08-2005. ) Laser Induced Fluorescence Detection System for Multi-Lane Plastic Microchips .Washington, DC.

C. Coombs, P.A. Limbach (07-2005. ) Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs .Ithaca, NY.

M. Hossain, P.A. Limbach (05-2005. ) Ribonuclease T1 Mediated Cleavage and MALDI-MS for Detection and Quantification of Escherichia coli tRNAs .San Antonio, TX.

A.L. Dupre, W.D. Dominick, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (03-2005. ) EOF Characterization of Surface-Modified PMMA Microchips for Use With Electrospray Ionization Mass Spectrometry .The Ohio State University, Columbus, OH .

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (03-2005. ) Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry .Ohio State University, Columbus, OH.

M. Hossain, P.A. Limbach (03-2005. ) Detection and Quantification of Escherichia coli tRNAs by MALDI-MS .Ohio State University, Columbus, OH.

C. Coombs, P.A. Limbach (03-2005. ) Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs .University of Cincinnati, Cincinnati, OH.

M.J. Suh, S. Pourshahian, P.A. Limbach (05-2004. ) Probing the Stability of Ribonucleoprotein Complexes by Mass Spectrometry .

W.D. Dominick, A. Dupre, L. Jin, J. Mecomber, P.A. Limbach (05-2004. ) Plastic Microchips for Mass Spectrometry .

A. Durairaj, K.G. Patteson, P.A. Limbach (03-2004. ) Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA .

Z. Meng, P.A. Limbach (03-2004. ) RNase Mapping of Intact Nucleic Acids by ESI-FTICRMS and 18O Labeling .

R. Tummala, K.B. Green-Church, P.A. Limbach (03-2004. ) Effect of Sodium Dodecyl Sulfate on MALDI-MS Analysis of Proteolytic Digests .

M.-J. Suh, P.A. Limbach (03-2004. ) Investigation of Methods Suitable for the MALDI Mass Spectrometric Analysis of Proteins from Ribonucleoprotein Complexes .

M.J. Suh, S. Pourshahian, P.A. Limbach (10-2003. ) Developing Cross-Linking and MALDI-TOFMS Approaches for Exploring Ribosomal Assembly and Interactions .

B.T. Berhane, P.A. Limbach (06-2003. ) Functional Microfabricated Devices for the Mass Spectral Analysis of Nucleic Acids .

S.N. Mounicou, J. Meija, P.A. Limbach, J.A. Caruso (06-2003. ) The Synergy of Tandem Atomic and Molecular Mass Spectrometry Approaches for Characterizing Metalloproteins .

C. Robins, P.A. Limbach (03-2003. ) Adding Selectivity to MALDI-MS for Nonpolar Analytes .

Collin Wetzel and Patrick A. Limbach (04-2012. ) High throughput global identification of tRNA through the targeted detection of unique transitions .Columbus, OH. Conference.

Collin Wetzel and Patrick A. Limbach (10-2012. ) Targeted Tandem Mass Spectrometry for tRNA Identification .Dayton, OH. Conference.

Wunan Shi, Collin Wetzel and Patrick A. Limbach (04-2012. ) High throughput De Novo Sequencing of Total tRNAs in Thermus thermophulis Using LC-MS/MS .Columbus, OH. Conference.

Xiaoyu Cao and Patrick A. Limbach (04-2013. ) Inosine at the Wobble Position of tRNAArg in Thermus thermophilus .Columbus, OH. Conference.

Yang Jiao and Patrick A. Limbach (04-2013. ) Ligating oligonucleotide and NAD+ with T4 RNA ligase .Columbus, OH. Conference.

Robert Ross and Patrick A. Limbach (04-2013. ) Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34 .Columbus, OH. Conference.

Collin Wetzel and Patrick A. Limbach (04-2013. ) Automated data mining for C13 depleted RNA .Columbus, OH. Conference.

Collin Wetzel and Patrick A. Limbach (06-2013. ) Targeted Tandem Mass Spectrometry for tRNA Identification .Minneapolis MN. Conference.

Sarah F.C. Soper, Hajin Kim, Sanjaya Abeysirigunawardena, Romel Dator, Taekjip Ha, Patrick Limbach, Megan Mayerle, Kaushik Ragunathan, Sarah Woodson (07-2013. ) Visualizing RNA Folding and Dynamics During Ribosome Assembly in Bacteria .Napa Valley, CA. Conference.

B. Tekaligne, B. Addepalli, P. A. Limbach (08-2013. ) Role of rRNA domains in bacterial ribosome subunit assembly .Cincinnati, OH. UC.

Event Organized

ASMS Sanibel Conference

ASMS Sanibel Conference Conference 01-2010 01-2010 St. Pete Beach, FL Level:International

2014 Oesper Symposium

2014 Oesper Symposium Symposium 10-24-2014 University of Cincinnati

Honors and Awards

1998 American Society for Mass Spectrometry Research Award Sponsored by Finnigan, LSU Status:Recipient

1998 Peter A. Soderbergh Award for Innovation in Teaching, LSU Status:Recipient

1999 Award of Excellence in Undergraduate Teaching, College of Basic Sciences, LSU Status:Recipient

1999 Nontenured Faculty Award in the Physical Sciences, Phi Kappa Phi, LSU Status:Recipient

2003 Ohio Eminent Scholar in Mass Spectrometry, UC Status:Recipient

2006 Fellow of the Graduate School, UC Status:Recipient

2008 Sigma Xi Young Investigator Research Award, UC Status:Recipient

2009 Cincinnati 2008 Chemist of the Year Award, Cincinnati Section American Chemical Society, UC Status:Recipient

2013 Fellow of the American Association for the Advancement of Science (AAAS)

2015 Hans H. Jaffé Faculty Award for Excellence (UC Chemistry)

Service

Journal of the American Society for Mass Spectrometry Editorial Board Member Type:Service to Professional Associations Level:International 01-2005 -12-2010

Analytica Chimica Acta Editorial Board Member Type:Service to Professional Associations Level:International 01-2005 -12-2015

National Institutes of Health (NIEHS P30 Center Grant Panel ) Review Board/Panel Type:Grant Panel Level:National 08-2010 -08-2010

Sigma Xi President Type:Service to Professional Associations Level:University 07-2012 -06-2013

Center for Clinical & Translational Science & Training (CCTST) (Internal Advisory Board ) Type:University/College Service Level:University 09-2010

Ohio State Proteomics Core Facility (Advisory Board External Member ) Type:University/College Service Level:State 09-2007

UC Faculty Senate (Committee on Research and Scholarship ) Committee Member Type:University/College Service Level:University 08-2012 -07-2014

National Institutes of Health (ZRR1 BT-7 Special Emphasis Panel ) Review Board/Panel Type:Grant Panel Level:National 02-21-2012 -02-22-2012

National Institutes of Health (ZRG1-IMST-Q P41 Review Panel ) Review Board/Panel Type:Grant Panel Level:National 06-18-2012 -06-19-2012

National Institutes of Health (ZRG1 BCMB-D Review Panel ) Review Board/Panel Type:Grant Panel Level:National 09-06-2012 -09-07-2012

National Institutes of Health Director’s Pioneer Award Review Board/Panel Type:Grant Panel 01-2013

National Institutes of Health, ZRG1 BBCB-A Panel Review Board/Panel Type:Grant Panel 04-2013

National Science Foundation CAREER Grant Evaluation Panel, Review Board/Panel Type:Grant Panel 09-2013

Sigma Xi (Immediate Past President ) Type:Service to Professional Associations Level:University 07-01-2013 -06-30-2014

Post Graduate Training and Education

1993-1994 Postdoctoral Fellow with Prof. James McCloskey, University of Utah, ,

Keywords

biological, RNA, transfer RNA, ribonucleic acids, protein translation, ribosome, mass spectrometry, modified nucleosides, LC-MS, LC/MS, protein mass spectrometry, ncRNA

Courses Taught

Topics in Biochemistry Level:Graduate

Topics in Biochemistry Level:Graduate

Topics in Biochemistry Level:Graduate

Fundamentals of Mass Spectrometry Level:Graduate

Intermediate Biochemistry: Synthesizing the Molecules of Life Level:Undergraduate

Thermodynamics and Molecular Interactions Level:Graduate

Intermediate Biochemistry: Synthesizing the Molecules of Life Level:Undergraduate

Biochemistry Laboratory: Independent Study Level:Undergraduate

Modern Topics in Biochemistry Level:Graduate

Thermodynamics and Molecular Interactions Level:Graduate

Intermediate Biochemistry: Synthesizing the Molecules of Life Level:Undergraduate

Thermodynamics and Molecular Interactions Level:Graduate

Faculty Development Activities

08-2012 -07-2014 New Faculty Institute External Mentor to New Faculty Member in Physics, CETL University of Cincinnati Type:Other

Patents and Inventions

62/151,546 CODON OPTIMIZED CYTIDINE-SPECIFIC RIBONUCLEASE Balasubrahmanyam Addepalli, Sarah Venus and Patrick A Limbach, Filed Co-Inventor

62/151,640 CODON OPTIMIZED URIDINE-SPECIFIC RIBONUCLEASE Balasubrahmanyam Addepalli, Nicholas P. Lesner and Patrick A. Limbach, Filed Co-Inventor

Contact Information

Academic - Cincinnati  Ohio, Phone: 513-558-0026
Pat.Limbach@uc.edu