Linde A Miles, PhD

I’m an assistant professor, scientist and researcher focused on acute myeloid leukemia, cancer evolution and cancer biology. My research examines and models clonal evolutionary trajectories that occur during leukemogenesis using single-cell profiling of patient isolates and pre-clinical models. The foundation of my research relies on the intersection of single cell multi-omic patient data and the development of mouse models capable of selectively activating individual alleles (or more than one allele). These models will serve as a platform to probe the effects of genetic and chemical perturbation to identify clone-specific vulnerabilities that can be exploited for new therapeutic strategies.

B.S.: Pennsylvania State University University Park, PA, 2009 (Biochemistry & Molecular Biology)

Ph.D.: Johns Hopkins University School of Medicine Baltimore, MD, 2016 (Pharmacology & Molecular Sciences)

Peer Reviewed Publications

SanMiguel, Jennifer M; Eudy, Elizabeth; Loberg, Matthew A; Miles, Linde A; Stearns, Tim; Mistry, Jayna J; Rauh, Michael J; Levine, Ross L; Trowbridge, Jennifer J  (2022). Cell origin-dependent cooperativity of mutant Dnmt3a and Npm1 in clonal hematopoiesis and myeloid malignancy. Blood advances, 6 (12) , 3666-3677More Information

Luu, Anh Phuong; Yao, Zhenlan; Ramachandran, Sangeetha; Azzopardi, Stephanie A; Miles, Linde A; Schneider, William M; Hoffmann, H-Heinrich; Bozzacco, Leonia; Garcia, Gustavo; Gong, Danyang; Damoiseaux, Robert; Tang, Hengli; Morizono, Kouki; Rudin, Charles M; Sun, Ren; Arumugaswami, Vaithilingaraja; Poirier, John T; MacDonald, Margaret R; Rice, Charles M; Li, Melody M H  (2021). A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry. Viruses, 13 (11) More Information

Jayawardena, Nadishka; Miles, Linde A; Burga, Laura N; Rudin, Charles; Wolf, Matthias; Poirier, John T; Bostina, Mihnea  (2021). N-Linked Glycosylation on Anthrax Toxin Receptor 1 Is Essential for Seneca Valley Virus Infection. Viruses, 13 (5) More Information

Hoffmann, H-Heinrich; Schneider, William M; Rozen-Gagnon, Kathryn; Miles, Linde A; Schuster, Felix; Razooky, Brandon; Jacobson, Eliana; Wu, Xianfang; Yi, Soon; Rudin, Charles M; MacDonald, Margaret R; McMullan, Laura K; Poirier, John T; Rice, Charles M  (2021). TMEM41B Is a Pan-flavivirus Host Factor. Cell, 184 (1) , 133-148.e20More Information

Xiao, Wenbin; Miles, Linde A; Bowman, Robert L; Durani, Vidushi; Tian, Helen S; DelGaudio, Nicole L; Mishra, Tanmay; Zhu, Menglei; Zhang, Yanming; Stump, Sarah E; Tallman, Martin S; Levine, Ross L; Cai, Sheng F  (2020). A JAK2/IDH1-mutant MPN clone unmasked by ivosidenib in an AML patient without antecedent MPN. Blood advances, 4 (23) , 6034-6038More Information

Miles, Linde A; Bowman, Robert L; Merlinsky, Tiffany R; Csete, Isabelle S; Ooi, Aik T; Durruthy-Durruthy, Robert; Bowman, Michael; Famulare, Christopher; Patel, Minal A; Mendez, Pedro; Ainali, Chrysanthi; Demaree, Benjamin; Delley, Cyrille L; Abate, Adam R; Manivannan, Manimozhi; Sahu, Sombeet; Goldberg, Aaron D; Bolton, Kelly L; Zehir, Ahmet; Rampal, Raajit; Carroll, Martin P; Meyer, Sara E; Viny, Aaron D; Levine, Ross L  (2020). Single-cell mutation analysis of clonal evolution in myeloid malignancies. Nature, 587 (7834) , 477-482More Information

Hoffmann, H-Heinrich; Schneider, William M; Rozen-Gagnon, Kathryn; Miles, Linde A; Schuster, Felix; Razooky, Brandon; Jacobson, Eliana; Wu, Xianfang; Yi, Soon; Rudin, Charles M; MacDonald, Margaret R; McMullan, Laura K; Poirier, John T; Rice, Charles M  (2020). TMEM41B is a pan-flavivirus host factor. bioRxiv : the preprint server for biologyMore Information

Loberg, Matthew A; Bell, Rebecca K; Goodwin, Leslie O; Eudy, Elizabeth; Miles, Linde A; SanMiguel, Jennifer M; Young, Kira; Bergstrom, David E; Levine, Ross L; Schneider, Rebekka K; Trowbridge, Jennifer J  (2019). Sequentially inducible mouse models reveal that Npm1 mutation causes malignant transformation of Dnmt3a-mutant clonal hematopoiesis. Leukemia, 33 (7) , 1635-1649More Information

Yao, Zhan; Yaeger, Rona; Rodrik-Outmezguine, Vanessa S; Tao, Anthony; Torres, Neilawattie M; Chang, Matthew T; Drosten, Matthias; Zhao, Huiyong; Cecchi, Fabiola; Hembrough, Todd; Michels, Judith; Baumert, Hervé; Miles, Linde; Campbell, Naomi M; de Stanchina, Elisa; Solit, David B; Barbacid, Mariano; Taylor, Barry S; Rosen, Neal  (2017). Tumours with class 3 BRAF mutants are sensitive to the inhibition of activated RAS. Nature, 548 (7666) , 234-238More Information

Miles, Linde A; Burga, Laura N; Gardner, Eric E; Bostina, Mihnea; Poirier, John T; Rudin, Charles M  (2017). Anthrax toxin receptor 1 is the cellular receptor for Seneca Valley virus. The Journal of clinical investigation, 127 (8) , 2957-2967More Information

Gardner, Eric E; Lok, Benjamin H; Schneeberger, Valentina E; Desmeules, Patrice; Miles, Linde A; Arnold, Paige K; Ni, Andy; Khodos, Inna; de Stanchina, Elisa; Nguyen, Thuyen; Sage, Julien; Campbell, John E; Ribich, Scott; Rekhtman, Natasha; Dowlati, Afshin; Massion, Pierre P; Rudin, Charles M; Poirier, John T  (2017). Chemosensitive Relapse in Small Cell Lung Cancer Proceeds through an EZH2-SLFN11 Axis. Cancer cell, 31 (2) , 286-299More Information

Miles, Linde A; Garippa, Ralph J; Poirier, John T  (2016). Design, execution, and analysis of pooled in vitro CRISPR/Cas9 screens. The FEBS journal, 283 (17) , 3170-80More Information

Weiskopf, Kipp; Jahchan, Nadine S; Schnorr, Peter J; Cristea, Sandra; Ring, Aaron M; Maute, Roy L; Volkmer, Anne K; Volkmer, Jens-Peter; Liu, Jie; Lim, Jing Shan; Yang, Dian; Seitz, Garrett; Nguyen, Thuyen; Wu, Di; Jude, Kevin; Guerston, Heather; Barkal, Amira; Trapani, Francesca; George, Julie; Poirier, John T; Gardner, Eric E; Miles, Linde A; de Stanchina, Elisa; Lofgren, Shane M; Vogel, Hannes; Winslow, Monte M; Dive, Caroline; Thomas, Roman K; Rudin, Charles M; van de Rijn, Matt; Majeti, Ravindra; Garcia, K Christopher; Weissman, Irving L; Sage, Julien  (2016). CD47-blocking immunotherapies stimulate macrophage-mediated destruction of small-cell lung cancer. The Journal of clinical investigation, 126 (7) , 2610-20More Information

Miles, Linde A; Brennen, W Nathaniel; Rudin, Charles M; Poirier, John T  (2015). Correction: Seneca Valley Virus 3Cpro Substrate Optimization Yields Efficient Substrates for use in Peptide-Prodrug Therapy. PloS one, 10 (8) , e0136480More Information

Miles, Linde A; Brennen, W Nathaniel; Rudin, Charles M; Poirier, John T  (2015). Seneca Valley Virus 3Cpro Substrate Optimization Yields Efficient Substrates for Use in Peptide-Prodrug Therapy. PloS one, 10 (6) , e0129103More Information

Matthews, Megan L; Chang, Wei-chen; Layne, Andrew P; Miles, Linde A; Krebs, Carsten; Bollinger, J Martin  (2014). Direct nitration and azidation of aliphatic carbons by an iron-dependent halogenase. Nature chemical biology, 10 (3) , 209-15More Information

Matthews, Megan L; Neumann, Christopher S; Miles, Linde A; Grove, Tyler L; Booker, Squire J; Krebs, Carsten; Walsh, Christopher T; Bollinger, J Martin  (2009). Substrate positioning controls the partition between halogenation and hydroxylation in the aliphatic halogenase, SyrB2. Proceedings of the National Academy of Sciences of the United States of America, 106 (42) , 17723-8More Information

2017-2022 Postdoctoral Fellowship, Clonal evolution of acute myeloid leukemia under mentorship of Dr. Ross Levine, Memorial Sloan Kettering Cancer Center, , New York, NY

Academic - Cincinnati Children's Hospital Medical Center
240 Albert Sabin Way
Cincinnati   Ohio, 45229
Phone: 513-636-3074
milesll@ucmail.uc.edu