Douglas Millay , PhD

The Millay Lab is interested in understanding how precursor cells fuse to form multi-nucleated skeletal muscle. We recently discovered a necessary component (named myomaker) of the muscle fusion machinery. The lab's goal is to delineate the mechanisms by which this multi-pass membrane protein directs cell-cell fusion and manipulate muscle cell fusion as a strategy for in vivo cell therapy.

Peer Reviewed Publications

Reid, Andrea L; Wang, Yimin; Samani, Adrienne; Hightower, Rylie M; Lopez, Michael A; Gilbert, Shawn R; Ianov, Lara; Crossman, David K; Dell'Italia, Louis J; Millay, Douglas P; van Groen, Thomas; Halade, Ganesh V; Alexander, Matthew S (2020. ) DOCK3 is a dosage-sensitive regulator of skeletal muscle and Duchenne muscular dystrophy-associated pathologies.Human molecular genetics, , 29 (17 ) ,2855-2871 More Information

Petrany, Michael J; Song, Taejeong; Sadayappan, Sakthivel; Millay, Douglas P (2020. ) Myocyte-derived Myomaker expression is required for regenerative fusion but exacerbates membrane instability in dystrophic myofibers.JCI insight, , 5 (9 ) , More Information

Nikolaou, Sia; Cramer, Alyssa Aw; Hu, Liangjun; Goh, Qingnian; Millay, Douglas P; Cornwall, Roger (2019. ) Proteasome inhibition preserves longitudinal growth of denervated muscle and prevents neonatal neuromuscular contractures.JCI insight, , 4 (23 ) , More Information

Aulet, Ricardo Mario; Trieu, Vanessa; Landrigan, Gary P; Millay, Donna J (2019. ) Changes in Opioid Prescribing Habits for Patients Undergoing Rhinoplasty and Septoplasty.JAMA facial plastic surgery, , 21 (6 ) ,487-490 More Information

Petrany, Michael J; Millay, Douglas P (2019. ) Cell Fusion: Merging Membranes and Making Muscle.Trends in cell biology, , 29 (12 ) ,964-973 More Information

Goh, Qingnian; Song, Taejeong; Petrany, Michael J; Cramer, Alyssa Aw; Sun, Chengyi; Sadayappan, Sakthivel; Lee, Se-Jin; Millay, Douglas P (2019. ) Myonuclear accretion is a determinant of exercise-induced remodeling in skeletal muscle.eLife, , 8 , More Information

Song, Taejeong; Manoharan, Palanikumar; Millay, Douglas P; Koch, Sheryl E; Rubinstein, Jack; Heiny, Judith A; Sadayappan, Sakthivel (2019. ) Dilated cardiomyopathy-mediated heart failure induces a unique skeletal muscle myopathy with inflammation.Skeletal muscle, , 9 (1 ) ,4 More Information

Leikina, Evgenia; Gamage, Dilani G; Prasad, Vikram; Goykhberg, Joanna; Crowe, Michael; Diao, Jiajie; Kozlov, Michael M; Chernomordik, Leonid V; Millay, Douglas P (2018. ) Myomaker and Myomerger Work Independently to Control Distinct Steps of Membrane Remodeling during Myoblast Fusion.Developmental cell, , 46 (6 ) ,767-780.e7 More Information

Sampath, Srihari C; Sampath, Srinath C; Millay, Douglas P (2018. ) Myoblast fusion confusion: the resolution begins.Skeletal muscle, , 8 (1 ) ,3 More Information

Gamage, Dilani G; Leikina, Eugenia; Quinn, Malgorzata E; Ratinov, Anthony; Chernomordik, Leonid V; Millay, Douglas P (2017. ) Insights into the localization and function of myomaker during myoblast fusion.The Journal of biological chemistry, , 292 (42 ) ,17272-17289 More Information

Quinn, Malgorzata E; Goh, Qingnian; Kurosaka, Mitsutoshi; Gamage, Dilani G; Petrany, Michael J; Prasad, Vikram; Millay, Douglas P (2017. ) Myomerger induces fusion of non-fusogenic cells and is required for skeletal muscle development.Nature communications, , 8 ,15665 More Information

Goh, Qingnian; Millay, Douglas P (2017. ) Requirement of myomaker-mediated stem cell fusion for skeletal muscle hypertrophy.eLife, , 6 , More Information

Mitani, Yasuyuki; Vagnozzi, Ronald J; Millay, Douglas P (2017. ) In vivo myomaker-mediated heterologous fusion and nuclear reprogramming.FASEB journal : official publication of the Federation of American Societies for Experimental Biology, , 31 (1 ) ,400-411 More Information

Zeve, Daniel; Millay, Douglas P; Seo, Jin; Graff, Jonathan M (2016. ) Exercise-Induced Skeletal Muscle Adaptations Alter the Activity of Adipose Progenitor Cells.PloS one, , 11 (3 ) ,e0152129 More Information

Millay, Douglas P; Gamage, Dilani G; Quinn, Malgorzata E; Min, Yi-Li; Mitani, Yasuyuki; Bassel-Duby, Rhonda; Olson, Eric N (2016. ) Structure-function analysis of myomaker domains required for myoblast fusion.Proceedings of the National Academy of Sciences of the United States of America, , 113 (8 ) ,2116-21 More Information

Millay, Douglas P; Sutherland, Lillian B; Bassel-Duby, Rhonda; Olson, Eric N (2014. ) Myomaker is essential for muscle regeneration.Genes & development, , 28 (15 ) ,1641-6 More Information

Burr, Adam R; Millay, Douglas P; Goonasekera, Sanjeewa A; Park, Ki Ho; Sargent, Michelle A; Collins, James; Altamirano, Francisco; Philipson, Kenneth D; Allen, Paul D; Ma, Jianjie; López, José Rafael; Molkentin, Jeffery D (2014. ) Na+ dysregulation coupled with Ca2+ entry through NCX1 promotes muscular dystrophy in mice.Molecular and cellular biology, , 34 (11 ) ,1991-2002 More Information

Millay, Douglas P; O'Rourke, Jason R; Sutherland, Lillian B; Bezprozvannaya, Svetlana; Shelton, John M; Bassel-Duby, Rhonda; Olson, Eric N (2013. ) Myomaker is a membrane activator of myoblast fusion and muscle formation.Nature, , 499 (7458 ) ,301-5 More Information

Millay, Douglas P; Olson, Eric N (2013. ) Making muscle or mitochondria by selective splicing of PGC-1?.Cell metabolism, , 17 (1 ) ,3-4 More Information

Hatley, Mark E; Tang, Wei; Garcia, Matthew R; Finkelstein, David; Millay, Douglas P; Liu, Ning; Graff, Jonathan; Galindo, Rene L; Olson, Eric N (2012. ) A mouse model of rhabdomyosarcoma originating from the adipocyte lineage.Cancer cell, , 22 (4 ) ,536-46 More Information

Zeve, Daniel; Seo, Jin; Suh, Jae Myoung; Stenesen, Drew; Tang, Wei; Berglund, Eric D; Wan, Yihong; Williams, Linda J; Lim, Ajin; Martinez, Myrna J; McKay, Renée M; Millay, Douglas P; Olson, Eric N; Graff, Jonathan M (2012. ) Wnt signaling activation in adipose progenitors promotes insulin-independent muscle glucose uptake.Cell metabolism, , 15 (4 ) ,492-504 More Information

Pei, Jimin; Millay, Douglas P; Olson, Eric N; Grishin, Nick V (2011. ) CREST--a large and diverse superfamily of putative transmembrane hydrolases.Biology direct, , 6 ,37 More Information

Goonasekera, Sanjeewa A; Lam, Chi K; Millay, Douglas P; Sargent, Michelle A; Hajjar, Roger J; Kranias, Evangelia G; Molkentin, Jeffery D (2011. ) Mitigation of muscular dystrophy in mice by SERCA overexpression in skeletal muscle.The Journal of clinical investigation, , 121 (3 ) ,1044-52 More Information

Wansapura, Janaka P; Millay, Douglas P; Dunn, R Scott; Molkentin, Jeffery D; Benson, D Woodrow (2011. ) Magnetic resonance imaging assessment of cardiac dysfunction in ?-sarcoglycan null mice.Neuromuscular disorders : NMD, , 21 (1 ) ,68-73 More Information

Wissing, Erin R; Millay, Douglas P; Vuagniaux, Grégoire; Molkentin, Jeffery D (2010. ) Debio-025 is more effective than prednisone in reducing muscular pathology in mdx mice.Neuromuscular disorders : NMD, , 20 (11 ) ,753-60 More Information

Millay, Douglas P; Goonasekera, Sanjeewa A; Sargent, Michelle A; Maillet, Marjorie; Aronow, Bruce J; Molkentin, Jeffery D (2009. ) Calcium influx is sufficient to induce muscular dystrophy through a TRPC-dependent mechanism.Proceedings of the National Academy of Sciences of the United States of America, , 106 (45 ) ,19023-8 More Information

Millay, Douglas P; Maillet, Marjorie; Roche, Joseph A; Sargent, Michelle A; McNally, Elizabeth M; Bloch, Robert J; Molkentin, Jeffery D (2009. ) Genetic manipulation of dysferlin expression in skeletal muscle: novel insights into muscular dystrophy.The American journal of pathology, , 175 (5 ) ,1817-23 More Information

Millay, Douglas P; Sargent, Michelle A; Osinska, Hanna; Baines, Christopher P; Barton, Elisabeth R; Vuagniaux, Grégoire; Sweeney, H Lee; Robbins, Jeffrey; Molkentin, Jeffery D (2008. ) Genetic and pharmacologic inhibition of mitochondrial-dependent necrosis attenuates muscular dystrophy.Nature medicine, , 14 (4 ) ,442-7 More Information

Parsons, Stephanie A; Millay, Douglas P; Sargent, Michelle A; Naya, Francisco J; McNally, Elizabeth M; Sweeney, H Lee; Molkentin, Jeffery D (2007. ) Genetic disruption of calcineurin improves skeletal muscle pathology and cardiac disease in a mouse model of limb-girdle muscular dystrophy.The Journal of biological chemistry, , 282 (13 ) ,10068-78 More Information

Parsons, Stephanie A; Millay, Douglas P; Sargent, Michelle A; McNally, Elizabeth M; Molkentin, Jeffery D (2006. ) Age-dependent effect of myostatin blockade on disease severity in a murine model of limb-girdle muscular dystrophy.The American journal of pathology, , 168 (6 ) ,1975-85 More Information

Parsons, Stephanie A; Millay, Douglas P; Wilkins, Benjamin J; Bueno, Orlando F; Tsika, Gretchen L; Neilson, Joel R; Liberatore, Christine M; Yutzey, Katherine E; Crabtree, Gerald R; Tsika, Richard W; Molkentin, Jeffery D (2004. ) Genetic loss of calcineurin blocks mechanical overload-induced skeletal muscle fiber type switching but not hypertrophy.The Journal of biological chemistry, , 279 (25 ) ,26192-200 More Information

Hindi, Sajedah M; Millay, Douglas P (2022. ) All for One and One for All: Regenerating Skeletal Muscle.Cold Spring Harbor perspectives in biology, , 14 (8 ) , More Information

Millay, Douglas P (2022. ) Regulation of the myoblast fusion reaction for muscle development, regeneration, and adaptations.Experimental cell research, , 415 (2 ) ,113134 More Information

Eigler, Tamar; Zarfati, Giulia; Amzallag, Emmanuel; Sinha, Sansrity; Segev, Nadav; Zabary, Yishaia; Zaritsky, Assaf; Shakked, Avraham; Umansky, Kfir-Baruch; Schejter, Eyal D; Millay, Douglas P; Tzahor, Eldad; Avinoam, Ori (2021. ) ERK1/2 inhibition promotes robust myotube growth via CaMKII activation resulting in myoblast-to-myotube fusion.Developmental cell, , 56 (24 ) ,3349-3363.e6 More Information

Bengtsen, Mads; Winje, Ivan Myhre; Eftestøl, Einar; Landskron, Johannes; Sun, Chengyi; Nygård, Kamilla; Domanska, Diana; Millay, Douglas P; Meza-Zepeda, Leonardo A; Gundersen, Kristian (2021. ) Comparing the epigenetic landscape in myonuclei purified with a PCM1 antibody from a fast/glycolytic and a slow/oxidative muscle.PLoS genetics, , 17 (11 ) ,e1009907 More Information

Prasad, Vikram; Millay, Douglas P (2021. ) Skeletal muscle fibers count on nuclear numbers for growth.Seminars in cell & developmental biology, , 119 ,3-10 More Information

Yaseen, Wesal; Kraft-Sheleg, Ortal; Zaffryar-Eilot, Shelly; Melamed, Shay; Sun, Chengyi; Millay, Douglas P; Hasson, Peleg (2021. ) Fibroblast fusion to the muscle fiber regulates myotendinous junction formation.Nature communications, , 12 (1 ) ,3852 More Information

Eder, Milton Mickey; Millay, Tamara A; Cottler, Linda B (2021. ) A compendium of community engagement responses to the COVID-19 pandemic.Journal of clinical and translational science, , 5 (1 ) ,e133 More Information

Milewski, David; Shukla, Samriddhi; Gryder, Berkley E; Pradhan, Arun; Donovan, Johnny; Sudha, Parvathi; Vallabh, Sushmitha; Pyros, Athena; Xu, Yan; Barski, Artem; Szabo, Sara; Turpin, Brian; Pressey, Joseph G; Millay, Douglas P; Khan, Javed; Kalinichenko, Vladimir V; Kalin, Tanya V (2021. ) FOXF1 is required for the oncogenic properties of PAX3-FOXO1 in rhabdomyosarcoma.Oncogene, , 40 (12 ) ,2182-2199 More Information

Girardi, Francesco; Taleb, Anissa; Ebrahimi, Majid; Datye, Asiman; Gamage, Dilani G; Peccate, Cécile; Giordani, Lorenzo; Millay, Douglas P; Gilbert, Penney M; Cadot, Bruno; Le Grand, Fabien (2021. ) TGF? signaling curbs cell fusion and muscle regeneration.Nature communications, , 12 (1 ) ,750 More Information

Golani, Gonen; Leikina, Evgenia; Melikov, Kamran; Whitlock, Jarred M; Gamage, Dilani G; Luoma-Overstreet, Gracia; Millay, Douglas P; Kozlov, Michael M; Chernomordik, Leonid V (2021. ) Myomerger promotes fusion pore by elastic coupling between proximal membrane leaflets and hemifusion diaphragm.Nature communications, , 12 (1 ) ,495 More Information

Petrany, Michael J; Swoboda, Casey O; Sun, Chengyi; Chetal, Kashish; Chen, Xiaoting; Weirauch, Matthew T; Salomonis, Nathan; Millay, Douglas P (2020. ) Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers.Nature communications, , 11 (1 ) ,6374 More Information

Hansson, Kenth-Arne; Eftestøl, Einar; Bruusgaard, Jo C; Juvkam, Inga; Cramer, Alyssa W; Malthe-Sørenssen, Anders; Millay, Douglas P; Gundersen, Kristian (2020. ) Myonuclear content regulates cell size with similar scaling properties in mice and humans.Nature communications, , 11 (1 ) ,6288 More Information

Cramer, Alyssa A W; Prasad, Vikram; Eftestøl, Einar; Song, Taejeong; Hansson, Kenth-Arne; Dugdale, Hannah F; Sadayappan, Sakthivel; Ochala, Julien; Gundersen, Kristian; Millay, Douglas P (2020. ) Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains.Nature communications, , 11 (1 ) ,6287 More Information

Leikina, Evgenia; Melikov, Kamran; Rabinovich, Anthony G; Millay, Douglas P; Chernomordik, Leonid V (2020. ) Lipid Mixing Assay for Murine Myoblast Fusion and Other Slow Cell-cell Fusion Processes.Bio-protocol, , 10 (5 ) ,e3544 More Information

Academic - CCHMC S Building
240 Albert Sabin Way
Cincinnati  Ohio, 45229
Phone: 513-803-743
douglas.millay@cchmc.org