Dorothy M. Supp , PHD

Areas of Specialization - bioengineering of human skin for improved wound healing, cutaneous gene therapy, molecular analysis of abnormal scarring, in vitro models for skin cancer research

Bachelor's Degree: Cornell University, College of Agriculture and Life Sciences Ithaca, New York,

Doctoral Degree: University of Cincinnati College of Medicine Cincinnati, Ohio, 1994 (Developmental Biology)

Fellowship: Childrens Hospital Research Foundation Cincinnnati, 1997 (Postdoctoral )

Fellowship: Shriners Hospitals for Children Cincinnati, OH , (Research Fellowship )

The purpose of my research is to enhance the performance of cultured skin substitutes for the healing of burns and other skin wounds. Cultured skin substitutes have been used at the Shriners Burns Hospital and the University of Cincinnati as an adjunctive treatment for large burn wounds. Although significant improvements over recent years have led to better clinical outcomes, cultured skin substitutes are still limited by deficiencies in anatomy and physiology compared to native skin autograft. Using the tools of molecular biology, my laboratory can genetically modify the cells used to prepare cultured skin to modulate expression of genes involved in wound healing. Genetic modification can theoretically be used to improve the composition or performance of skin substitutes, making them more similar to native skin autograft and increasing their clinical efficacy. My lab is pursuing several lines of investigation involving cultured skin substitutes. These include: ·Comparing the gene expression profiles of cultured skin and native human skin, using cDNA microarrays. This will enable us to better characterize the cultured skin substitutes and focus on specific genes and/or pathways for regulation through genetic modification. ·Identifying safe and efficient methods for genetic modification of skin cells. ·Preparing genetically modified skin substitutes and evaluating their performance in a preclinical animal model. ·Examining expression of antimicrobial peptides, such as defensins, in cultured skin grafts, to determine the feasibility of increasing resistance to microbial contamination. ·Grafting of allogeneic skin substitutes for treatment of chronic leg ulcers. ·Studying the effects of mutations in the melanocortin 1 receptor, a candidate melanoma-susceptibility gene, in melanocytes in organotypic culture, to determine if they show increased sensitivity to UV-induced DNA damage.

2021 - Adjunct Professor, Department of Surgery, Division of Plastic, Reconstructive, and Hand Surgery/Burn Surgery, University of Cincinnati College of Medicine,

Grant: #R01 GM079542 Investigators:Bailey, John; Boyce, Steven; Kagan, Richard; Supp, Dorothy; Warner, Petra; Yakuboff, Kevin 09-04-2007 -08-31-2012 National Institute of General Medical Sciences Somatic Stem C ells in Engineered Human Skin Role:Collaborator $889,200.00 Active Level:Federal

Grant: #AFIRM Investigators:Boyce, Steven; Supp, Dorothy 03-10-2008 -06-30-2013 Department of the Army Medical Research and Materiel Command Armed Forces Institute For Regenerative Medicine (AFIRM) Role:Collaborator $1,224,151.00 Active Level:Federal

Grant: #TECH 09-006 Investigators:Boyce, Steven; Supp, Dorothy 08-01-2008 -06-30-2012 Ohio Department of Development Engineered Skin Substitutes Containing Mesenchymal Stem Cells Role:Collaborator $750,000.00 Active Level:State of Ohio

Grant: #R01 ES017561 Investigators:Abdelmalek, Zalfa; Kadekaro, Ana Luisa; Supp, Dorothy 07-20-2009 -04-30-2014 National Institute of Environmental Health Sciences Impact of MC 1R Functional Variants on the DNA Damage Response of Human Melanocytes Role:Collaborator $1,795,727.00 Active Level:Federal

Grant: #3 R01 GM079542-03S1 Investigators:Boyce, Steven; Supp, Dorothy 09-30-2009 -08-31-2012 National Institute of General Medical Sciences R01 GM79542 Somatic Stem Cells in Engineered Human Skin Administrative Supplement Role:Collaborator $246,973.00 Active Level:Federal

Grant: #URC Interdisciplinary Award AY2015-16 Investigators:Boyce, Steven; Supp, Dorothy; Zhang, Yuhang 05-01-2016 -04-30-2017 UC's University Research Council ENGINEERING HAIR FOLLICLE REGENERATION BY TARGETING DERMAL SHEATH CELLS Role:Collaborator $25,000.00 Active Level:Internal UC

Grant: #R21AR0173380 Investigators:Boyce, Steven; Supp, Dorothy; Zhang, Yuhang 04-01-2018 -03-31-2020 National Institute of Arthritis, Musculoskeletal and Skin Disease Reprogramming the dermal microenvironment to induce hair follicle neogenesis in engineered skin Role:Collaborator $211,043.00 Awarded Level:Federal

Grant: #VA IPA Supp Investigators:Supp, Dorothy 04-01-2020 -03-31-2021 Department of Veterans Affairs VA IPA Supp Role:PI $8,316.00 Active Level:Federal

Grant: #2020 Industry Sponsored Research Agreement Investigators:Supp, Dorothy 12-01-2020 -05-30-2021 Redx Pharma Plc Collaboration to determine efficacy of Redx Pharma proprietary agents as treatments for Keloids Role:PI $79,032.65 Awarded Level:Foreign Industry

Grant: #Shriners 72005-CIN-21 Investigators:Supp, Dorothy 01-01-2021 -12-31-2021 Shriners Hospitals for Children - Cincinnati The Role of Vitamin D and the Vitamin D Receptor in Fibrotic Wound Healing Role:PI $257,935.00 Awarded Level:Private Non-Profit

Grant: #Shriners Epidermolysis Investigators:Supp, Dorothy 01-01-2021 -12-31-2021 Shriners Hospital for Children - International Headquarters Correction of Epidermolysis Bullosa via Genome Editing and Tissue Engineering Role:PI $224,472.00 Active Level:Private Non-Profit

Grant: #Shriners 71012-CIN-20 Investigators:Supp, Dorothy 01-01-2021 -12-31-2021 Shriners Hospital for Children - International Headquarters Personalized Ablative Laser Therapy: A New Strategy for Effective Scar Reduction Role:PI $74,226.00 Active Level:Private Non-Profit

Grant: #PR221734P1 Investigators:Supp, Dorothy 09-01-2023 -08-31-2026 Department of the Army Medical Research Acquisition Activity Tissue Engineering for Cutaneous and Systemic Epidermolysis Bullosa Gene Therapy Role:PI 1558753.00 Hold Level:Federal

Grant: #R01AR077238 Investigators:Supp, Dorothy; Xie, Changchun; Zhang, Yuhang 02-04-2021 -11-30-2025 National Institute of Arthritis, Musculoskeletal and Skin Disease Multiscale modeling of an inductive hair follicle microenvironment in engineered skin substitute Role:Collaborator $364,613.00 Awarded Level:Federal

Grant: #TBD / R01AR080946 Investigators:Supp, Dorothy 08-15-2023 -06-30-2024 National Institute of Arthritis, Musculoskeletal and Skin Disease In Situ Skin Regeneration for Full Thickness Burn Injuries Role:PI 66465.77 Hold Level:Federal

Grant: #R01AR077238 Investigators:Supp, Dorothy; Xie, Changchun; Zhang, Yuhang 02-01-2021 -11-30-2025 National Institute of Arthritis, Musculoskeletal and Skin Disease Multiscale modeling of an inductive hair follicle microenvironment in engineered skin substitute Role:Collaborator $364,613.00 Awarded Level:Federal

Grant: #prepare reverse agreement_TECG20232883 Investigators:Supp, Dorothy 09-25-2023 -09-24-2024 Ohio Department of Development Development of LK 467, a tetrapeptide MC1R agonist, for sunless tanning Role:PI 120000.00 Hold Level:Ohio, State of

Grant: #R21AR078976 Investigators:Boyce, Steven; Supp, Dorothy; Zhang, Yuhang 04-12-2021 -03-31-2023 National Institute of Arthritis, Musculoskeletal and Skin Disease Reprogramming of human fibroblasts into inductive dermal papilla cells Role:Collaborator 213345.00 Awarded Level:Federal

Grant: #MC1R (R41AR083311) Investigators:Supp, Dorothy 09-22-2023 -08-14-2024 National Institute of Arthritis, Musculoskeletal and Skin Disease Vitiligo topical treatment applying a potent, highly selective MC1R agonist Role:PI 88777.00 Hold Level:Federal

Grant: #Shriners 71003 Investigators:Supp, Dorothy 01-01-2022 -12-31-2024 Shriners Hospital for Children - International Headquarters In Situ Skin Regeneration for Full Thickness Burn Injuries Role:PI 45581.00 Hold Level:Non Profit

Grant: #GR133079 Investigators:Supp, Dorothy 08-15-2023 -06-30-2028 National Institute of Arthritis, Musculoskeletal and Skin Disease In Situ Skin Regeneration for Full Thickness Burn Injuries Role:PI 61543.00 Hold Level:Federal

Grant: #R21NS130138 Investigators:Berta, Temugin; Davidson, Steve; Supp, Dorothy 09-08-2022 -08-31-2024 National Institute of Neurological Disorders and Stroke Genetic and physiological comparison of native human sensory neurons and induced pluripotent stem cells differentiated to sensory neurons Role:Collaborator 445500.00 Awarded Level:Federal

Peer Reviewed Publications

Kim, Jayne Y; Willard, James J; Supp, Dorothy M; Roy, Sashwati; Gordillo, Gayle M; Sen, Chandan K; Powell, Heather M (2015. ) Burn Scar Biomechanics Following Pressure Garment Therapy.Plastic and reconstructive surgery, , More Information

Kim, J. Y.; Dunham, D. M.; Supp, D. M.; Sen, C. K.; Powell, H. M. (2016. ) Novel burn device for rapid, reproducible burn wound generation .Plast Reconstr Surg Glob Open, , 42 (2 ) ,384-391

Alexander, J Wesley; Supp, Dorothy M (2014. ) Role of Arginine and Omega-3 Fatty Acids in Wound Healing and Infection.Advances in wound care, , 3 (11 ) ,682-690 More Information

Hahn, J. M.; McFarland, K. L.; Combs, K. A.; Supp, D. M. (2016. ) Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-á1 .Burns Trauma, , 4 (2 ) ,30

Boyce, Steven T; Zimmerman, Rachel L; Supp, Dorothy M (2014. ) Tumorigenicity Testing in Athymic Mice of Cultured Human Melanocytes for Transplantation in Engineered Skin Substitutes.Cell transplantation, , More Information

Supp, D. M.; Hahn, J. M.; McFarland, K. L.; Combs, K. A.; Lee, K. S.; Inceoglu, B.; Wan, D.; Boyce, S. T.; Hammock, B. D. (2016. ) Soluble Epoxide Hydrolase Inhibition and Epoxyeicosatrienoic Acid Treatment Improve Vascularization of Engineered Skin Substitutes .Plast Reconstr Surg Glob Open, , 4 (12 ) ,e1151

Supp, Dorothy M; Hahn, Jennifer M; McFarland, Kevin L; Glaser, Kathryn Inhibition of hyaluronan synthase 2 reduces the abnormal migration rate of keloid keratinocytes.Journal of burn care & research : official publication of the American Burn Association, , 35 (1 ) ,84-92 More Information

Hahn, J. M.; Supp, D. M. (2017. ) Abnormal expression of the vitamin D receptor in keloid scars .Acta Biomater, , 43 (7 ) ,1506-1515

Osterburg, Andrew R; Hexley, Philip; Supp, Dorothy M; Robinson, Chad T; Noel, Greg; Ogle, Cora; Boyce, Steven T; Aronow, Bruce J; Babcock, George F (2013. ) Concerns over interspecies transcriptional comparisons in mice and humans after trauma.Proceedings of the National Academy of Sciences of the United States of America, , 110 (36 ) ,E3370 More Information

DeBruler, D. M.; Blackstone, B. N.; Baumann, M. E.; McFarland, K. L.; Wulff, B. C.; Wilgus, T. A.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2017. ) Inflammatory responses, matrix remodeling, and re-epithelialization after fractional CO2 laser treatment of scars .Acta Biomater, , 49 (7 ) ,675-685

Hahn, Jennifer M; Glaser, Kathryn; McFarland, Kevin L; Aronow, Bruce J; Boyce, Steven T; Supp, Dorothy M Keloid-derived keratinocytes exhibit an abnormal gene expression profile consistent with a distinct causal role in keloid pathology.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 21 (4 ) ,530-44 More Information

Boyce, S. T.; Lloyd, C. M.; Kleiner, M. C.; Swope, V. B.; Abdel-Malek, Z.; Supp, D. M. (2017. ) Restoration of cutaneous pigmentation by transplantation to mice of isogeneic human melanocytes in dermal-epidermal engineered skin substitutes .Acta Biomater, , 30 (6 ) ,531-540

Sriwiriyanont, Penkanok; Lynch, Kaari A; McFarland, Kevin L; Supp, Dorothy M; Boyce, Steven T (2013. ) Characterization of hair follicle development in engineered skin substitutes.PloS one, , 8 (6 ) ,e65664 More Information

Blackstone, B. N.; Kim, J. Y.; McFarland, K. L.; Sen, C. K.; Supp, D. M.; Bailey, J. K.; Powell, H. M. (2017. ) Scar formation following excisional and burn injuries in a red Duroc pig model .Wound Repair Regen., , 25 (4 ) ,618-631

Sriwiriyanont, Penkanok; Lynch, Kaari A; Maier, Elizabeth A; Hahn, Jennifer M; Supp, Dorothy M; Boyce, Steven T (2012. ) Morphogenesis of chimeric hair follicles in engineered skin substitutes with human keratinocytes and murine dermal papilla cells.Experimental dermatology, , 21 (10 ) ,783-5 More Information

Sethuraman, S. A.; Gottschlich, M. M.; Supp, D. M.; Khoury, J.; Kagan, R. J. (2017. ) Vitamin D biomarkers associated with abnormal scar formation in pediatric burn patients .Acta Biomater, , 1 (1 ) ,78-87

Swope, Viki B; Jameson, Joshua A; McFarland, Kevin L; Supp, Dorothy M; Miller, William E; McGraw, Dennis W; Patel, Mira A; Nix, Matthew A; Millhauser, Glenn L; Babcock, George F; Abdel-Malek, Zalfa A (2012. ) Defining MC1R regulation in human melanocytes by its agonist ?-melanocortin and antagonists agouti signaling protein and ?-defensin 3.The Journal of investigative dermatology, , 132 (9 ) ,2255-62 More Information

DeBruler, D. M.; Zbinden, J. C.; Baumann, M. E.; Blackstone, B. N.; Malara, M. M.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2018. ) Early cessation of pressure garment therapy results in scar contraction and thickening .J Burn Care Res, , 13 (6 ) ,e0197558

Boyce, Steven T; Rice, Rachel K; Lynch, Kaari A; Supp, Andrew P; Swope, Viki B; Kagan, Richard J; Supp, Dorothy M Assessment of replication rates of human keratinocytes in engineered skin substitutes grafted to athymic mice.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 20 (4 ) ,544-51 More Information

DeBruler, D. M.; Blackstone, B. N.; McFarland, K. L.; Baumann, M. E.; Supp, D. M.; Bailey, J. K.; Powell, H. M. (2018. ) Effect of skin graft thickness on scar development in a porcine burn model .J Burn Care Res, , 13 (17 ) ,10

Supp, Dorothy M; Hahn, Jennifer M; Glaser, Kathryn; McFarland, Kevin L; Boyce, Steven T (2012. ) Deep and superficial keloid fibroblasts contribute differentially to tissue phenotype in a novel in vivo model of keloid scar.Plastic and reconstructive surgery, , 129 (6 ) ,1259-71 More Information

Bailey, J. K.; Blackstone, B. N.; DeBruler, D. M.; Kim, J. Y.; Baumann, M. E.; McFarland, K. L.; Imeokparia, F. O.; Supp, D. M.; Powell, H. M. (2018. ) Effects of early combinatorial treatment of autologous split-thickness skin grafts in red duroc pig model using pulsed dye laser and fractional CO2 laser .Acta Biomater, , 50 (1 ) ,78-87

Supp, Dorothy M (2012. ) Genomic Reprogramming and Skin-Like Maturation of Engineered Human Skin Substitutes.Advances in wound care, , 1 (2 ) ,63-68 More Information

Blackstone, B. N.; Hahn, J. M.; McFarland, K. L.; DeBruler, D. M.; Supp, D. M.; Powell, H. M. (2018. ) Inflammatory response and biomechanical properties of coaxial scaffolds for engineered skin in vitro and post-grafting .Acta Biomater, , 80 (17 ) ,247-257

Supp, Dorothy M; Glaser, Kathryn; Hahn, Jennifer M; McFarland, Kevin L; Boyce, Steven T (2012. ) LETTER TO THE EDITOR Abnormal Responses of Keloid Tissue to Wounding Identified Using In Vitro Model System. Eplasty, , 12 ,e19

Malara, M. M.; Kim, J. Y.; Clark, J. A.; Blackstone, B. N.; Ruegsegger, M. A.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2018. ) Structural, Chemical, and Mechanical Properties of Pressure Garments as a Function of Simulated Use and Repeated Laundering .J Burn Care Res, , 39 (4 ) ,562-571

McFarland, Kevin L; Glaser, Kathryn; Hahn, Jennifer M; Boyce, Steven T; Supp, Dorothy M Culture medium and cell density impact gene expression in normal skin and abnormal scar-derived fibroblasts.Journal of burn care & research : official publication of the American Burn Association, , 32 (4 ) ,498-508 More Information

Supp, D. M. (2019. ) Animal Models for Studies of Keloid Scarring .Adv Wound Care (New Rochelle), , 8 (2 ) ,77-89

Powell, Heather M; McFarland, Kevin L; Butler, David L; Supp, Dorothy M; Boyce, Steven T (2010. ) Uniaxial strain regulates morphogenesis, gene expression, and tissue strength in engineered skin.Tissue engineering. Part A, , 16 (3 ) ,1083-92 More Information

Supp, D. M.; Hahn, J. M.; Combs, K. A.; McFarland, K. L.; Schwentker, A.; Boissy, R. E.; Boyce, S. T.; Powell, H. M.; Lucky, A. W. (2019. ) Collagen VII Expression Is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Formation in Bilayer Engineered Skin Substitutes .Burns, , 28 (9-10 ) ,1242-1256

Klingenberg, Jennifer M; McFarland, Kevin L; Friedman, Aaron J; Boyce, Steven T; Aronow, Bruce J; Supp, Dorothy M (2010. ) Engineered human skin substitutes undergo large-scale genomic reprogramming and normal skin-like maturation after transplantation to athymic mice.The Journal of investigative dermatology, , 130 (2 ) ,587-601 More Information

Hahn, J. M.; Combs, K. A.; Lloyd, C. M.; McFarland, K. L.; Boyce, S. T.; Supp, D. M. (2019. ) Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds .PLoS One, , 14 (3 ) ,e0213325

Supp, Dorothy M; Gardner, Jason; Klingenberg, Jennifer M; Neely, Alice N (2009. ) Antibiotic resistance in clinical isolates of Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus does not impact sensitivity to human beta defensin 4.Burns : journal of the International Society for Burn Injuries, , 35 (7 ) ,949-55 More Information

DeBruler, D. M.; Baumann, M. E.; Blackstone, B. N.; Zbinden, J. C.; McFarland, K. L.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2019. ) Role of Early Application of Pressure Garments following Burn Injury and Autografting .Plast Reconstr Surg, , 143 (2 ) ,310e-321e

McFarland, Kevin L; Klingenberg, Jennifer M; Boyce, Steven T; Supp, Dorothy M Expression of genes encoding antimicrobial proteins and members of the toll-like receptor/nuclear factor-kappaB pathways in engineered human skin.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 16 (4 ) ,534-41 More Information

Malara, M. M.; Blackstone, B. N.; Baumann, M. E.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2020. ) Cultured Epithelial Autograft Combined with Micropatterned Dermal Template Forms Rete Ridges In Vivo .Tissue Eng Part A, , 26 (21-22 ) ,1138-1146

Kalyanaraman, Balaji; Supp, Dorothy M; Boyce, Steven T (2008. ) Medium flow rate regulates viability and barrier function of engineered skin substitutes in perfusion culture.Tissue engineering. Part A, , 14 (5 ) ,583-93 More Information

Baumann, M. E.; DeBruler, D. M.; Blackstone, B. N.; Coffey, R. A.; Boyce, S. T.; Supp, D. M.; Bailey, J. K.; Powell, H. M. (2020. ) Direct comparison of reproducibility and reliability in quantitative assessments of burn scar properties .Burns, , 102 (3-4 ) ,287-297

Powell, Heather M; Supp, Dorothy M; Boyce, Steven T (2008. ) Influence of electrospun collagen on wound contraction of engineered skin substitutes.Biomaterials, , 29 (7 ) ,834-43 More Information

Boyce, S. T.; Supp, D. M.; Lloyd, C. M. (2020. ) Exogenous Keratinocyte Growth Factor Is Not Required for Pigmentation of Skin Substitutes with Three Isogeneic Cell Types .Tissue Eng Part A, , 26 (3-4 ) ,214-224

Fang, Cheng-Hui; Li, Bingguo; James, J Howard; Yahya, Ayesha; Kadeer, Nijiati; Guo, Xialing; Xiao, Chun; Supp, Dorothy M; Kagan, Richard J; Hasselgren, Per-Olof; Sheriff, Sulaiman (2007. ) GSK-3beta activity is increased in skeletal muscle after burn injury in rats.American journal of physiology. Regulatory, integrative and comparative physiology, , 293 (4 ) ,R1545-51 More Information

Blackstone, B. N.; Malara, M. M.; Baumann, M. E.; McFarland, K. L.; Supp, D. M.; Powell, H. M. (2020. ) Fractional CO2 laser micropatterning of cell-seeded electrospun collagen scaffolds enables rete ridge formation in 3D engineered skin .Acta Biomater, , 102 (3-4 ) ,287-297

Supp, Dorothy M (2007. ) Gene therapy to fight infection in skin transplants.Pharmacogenomics, , 8 (5 ) ,483-6 More Information

DeBruler, D. M.; Baumann, M. E.; Zbinden, J. C.; Blackstone, B. N.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2020. ) Improved Scar Outcomes with Increased Daily Duration of Pressure Garment Therapy .Adv Wound Care (New Rochelle), , 9 (8 ) ,453-61

Smiley, Andrea K; Gardner, Jason; Klingenberg, Jennifer M; Neely, Alice N; Supp, Dorothy M Expression of human beta defensin 4 in genetically modified keratinocytes enhances antimicrobial activity.Journal of burn care & research : official publication of the American Burn Association, , 28 (1 ) ,127-32 More Information

Supp, D. M.; Hahn, J. M.; Lloyd, C. M.; Combs, K. A.; Swope, V. B.; Abdel-Malek, Z.; Boyce, S. T. (2020. ) Light or Dark Pigmentation of Engineered Skin Substitutes Containing Melanocytes Protects Against Ultraviolet Light-Induced DNA Damage In Vivo .J Burn Care Res, , 41 (4 ) ,751-760

Smiley, Andrea K; Klingenberg, Jennifer M; Boyce, Steven T; Supp, Dorothy M (2006. ) Keratin expression in cultured skin substitutes suggests that the hyperproliferative phenotype observed in vitro is normalized after grafting.Burns : journal of the International Society for Burn Injuries, , 32 (2 ) ,135-8 More Information

Satish, L.; Evdokiou, A.; Geletu, E.; Hahn, J. M.; Supp, D. M. (2020. ) Pirfenidone inhibits epithelial-mesenchymal transition in keloid keratinocytes .Burns Trauma, , 8 (4 ) ,tkz007

Smiley, Andrea K; Klingenberg, Jennifer M; Aronow, Bruce J; Boyce, Steven T; Kitzmiller, W John; Supp, Dorothy M (2005. ) Microarray analysis of gene expression in cultured skin substitutes compared with native human skin.The Journal of investigative dermatology, , 125 (6 ) ,1286-301 More Information

Hahn, J. M.; McFarland, K. L.; Combs, K. A.; Anness, M. C.; Supp, D. M. (2021. ) Analysis of HOX gene expression and the effects of HOXA9 overexpression in fibroblasts derived from keloid lesions and normal skin .Wound Repair Regen, , 29 (5 ) ,777-791

Supp, Dorothy M; Boyce, Steven T Engineered skin substitutes: practices and potentials.Clinics in dermatology, , 23 (4 ) ,403-12 More Information

Gibson, A. L. F.; Carney, B. C.; Cuttle, L.; Andrews, C. J.; Kowalczewski, C. J.; Liu, A.; Powell, H. M.; Stone, R.; Supp, D. M.; Singer, A. J.; Shupp, J. W.; Stalter, L.; Moffatt, L. T. (2021. ) Coming to Consensus: What Defines Deep Partial Thickness Burn Injuries in Porcine Models? .J Burn Care Res, , 42 (1 ) ,98-109

Supp, Andrew P; Neely, Alice N; Supp, Dorothy M; Warden, Glenn D; Boyce, Steven T Evaluation of cytotoxicity and antimicrobial activity of Acticoat Burn Dressing for management of microbial contamination in cultured skin substitutes grafted to athymic mice. The Journal of burn care & rehabilitation, , 26 (3 ) ,238-46

Ruiz-Torres, S.; Brusadelli, M. G.; Witte, D. P.; Wikenheiser-Brokamp, K. A.; Sauter, S.; Nelson, A. S.; Sertorio, M.; Chlon, T. M.; Lane, A.; Mehta, P. A.; Myers, K. C.; Bedard, M. C.; Pal, B.; Supp, D. M.; Lambert, P. F.; Komurov, K.; Kovacic, M. B.; Davies, S. M.; Wells, S. I. (2021. ) Inherited DNA Repair Defects Disrupt the Structure and Function of Human Skin .Cell Stem Cell, , 28 (3 ) ,424-435.e6

Supp, Dorothy M; Karpinski, Andrea C; Boyce, Steven T (2004. ) Expression of human beta-defensins HBD-1, HBD-2, and HBD-3 in cultured keratinocytes and skin substitutes.Burns : journal of the International Society for Burn Injuries, , 30 (7 ) ,643-8 More Information

Supp, D. M.; Hahn, J. M.; Combs, K. A.; McFarland, K. L.; Powell, H. M. (2022. ) Isolation and feeder-free primary culture of four cell types from a single human skin sample .STAR Protoc, , 3 (1 ) ,101172

Supp, Dorothy M; Karpinski, Andrea C; Boyce, Steven T Vascular endothelial growth factor overexpression increases vascularization by murine but not human endothelial cells in cultured skin substitutes grafted to athymic mice. The Journal of burn care & rehabilitation, , 25 (4 ) ,337-45

Bergmann, C. B.; McReynolds, C. B.; Wan, D.; Singh, N.; Goetzman, H.; Caldwell, C. C.; Supp, D. M.; Hammock, B. D. (2022. ) sEH-derived metabolites of linoleic acid drive pathologic inflammation while impairing key innate immune cell function in burn injury .Proc Natl Acad Sci U S A, , 119 (13 ) ,e2120691119

Supp, Dorothy M; Wilson-Landy, Kaila; Boyce, Steven T (2002. ) Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology, , 16 (8 ) ,797-804 More Information

Supp, Dorothy M; Boyce, Steven T Overexpression of vascular endothelial growth factor accelerates early vascularization and improves healing of genetically modified cultured skin substitutes. The Journal of burn care & rehabilitation, , 23 (1 ) ,10-20

Supp, D M; Supp, A P; Bell, S M; Boyce, S T (2000. ) Enhanced vascularization of cultured skin substitutes genetically modified to overexpress vascular endothelial growth factor.The Journal of investigative dermatology, , 114 (1 ) ,5-13 More Information

Supp, D M; Bell, S M; Morgan, J R; Boyce, S T Genetic modification of cultured skin substitutes by transduction of human keratinocytes and fibroblasts with platelet-derived growth factor-A. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 8 (1 ) ,26-35

Boyce S.T.;Supp D.M.;Lloyd C.M. (02-01-2020. ) Exogenous Keratinocyte Growth Factor Is Not Required for Pigmentation of Skin Substitutes with Three.Tissue Engineering - Part A, , 26 (3-4 ) ,214-224 More Information

Blackstone B.;Malara M.;Baumann M.;McFarland K.;Supp D.;Powell H. (01-15-2020. ) Fractional CO2 laser micropatterning of cell-seeded electrospun collagen scaf.Acta Biomaterialia, , 102 ,287-297 More Information

Supp D.M.;Hahn J.M.;Combs K.A.;McFarland K.L.;Schwentker A.;Boissy R.E.;Boyce S.T.;Powell H.M.;Lucky A.W. (09-01-2019. ) Collagen VII Expression Is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Forma.Cell Transplantation, , 28 (9-10 ) ,1242-1256 More Information

Hahn J.M.;Combs K.A.;Lloyd C.M.;McFarland K.L.;Boyce S.T.;Supp D.M. (03-01-2019. ) Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting.PLoS ONE, , 14 (3 ) , More Information

Supp D. (02-01-2019. ) Animal Models for Studies of Keloid Scarring.Advances in Wound Care, , 8 (2 ) ,77-89 More Information

Debruler D.;Baumann M.;Blackstone B.;Zbinden J.;McFarland K.;Bailey J.;Supp D.;Powell H. (02-01-2019. ) Role of Early Application of Pressure Garments following Burn Injury and Autografting.Plastic and Reconstructive Surgery, , 143 (2 ) ,310e-321e More Information

Baumann M.E.;Blackstone B.N.;Malara M.M.;Clairmonte I.A.;Supp D.M.;Bailey J.K.;Powell H.M. (01-01-2019. ) Fractional CO2 laser ablation of porcine burn scars after grafting: Is deeper.Burns, , More Information

Blackstone B.;Hahn J.;McFarland K.;DeBruler D.;Supp D.;Powell H. (10-15-2018. ) Inflammatory response and biomechanical properties of coaxial scaffolds for engineered skin in vitro.Acta Biomaterialia, , 80 ,247-257 More Information

Malara M.;Kim J.;Clark J.;Blackstone B.;Ruegsegger M.;Bailey J.;Supp D.;Powell H. (06-13-2018. ) Structural, Chemical, and Mechanical Properties of Pressure Garments as a Function of Simulated Use .Journal of Burn Care and Research, , 39 (4 ) ,562-571 More Information

DeBruler D.;Blackstone B.;McFarland K.;Baumann M.;Supp D.;Bailey J.;Powell H. (06-01-2018. ) Effect of skin graft thickness on scar development in a porcine burn model.Burns, , 44 (4 ) ,917-930 More Information

DeBruler D.;Zbinden J.;Baumann M.;Blackstone B.;Malara M.;Bailey J.;Supp D.;Powell H. (06-01-2018. ) Early cessation of pressure garment therapy results in scar contraction and thickening.PLoS ONE, , 13 (6 ) , More Information

Bailey J.;Blackstone B.;DeBruler D.;Kim J.;Baumann M.;McFarland K.;Imeokparia F.;Supp D.;Powell H. (01-01-2018. ) Effects of early combinatorial treatment of autologous split-thickness skin grafts in red duroc pig .Lasers in Surgery and Medicine, , 50 (1 ) ,78-87 More Information

Hahn J.;Supp D. (11-01-2017. ) Abnormal expression of the vitamin D receptor in keloid scars.Burns, , 43 (7 ) ,1506-1515 More Information

Boyce S.;Lloyd C.;Kleiner M.;Swope V.;Abdel-Malek Z.;Supp D. (11-01-2017. ) Restoration of cutaneous pigmentation by transplantation to mice of isogeneic human melanocytes in d.Pigment Cell and Melanoma Research, , 30 (6 ) ,531-540 More Information

Lander J.;Supp D.;He H.;Martin L.;Chen X.;Weirauch M.;Boyce S.;Kopan R. (09-01-2017. ) Analysis of chromatin accessibility in human epidermis identifies putative barrier dysfunction-sensi.PLoS ONE, , 12 (9 ) , More Information

DeBruler D.;Blackstone B.;Baumann M.;McFarland K.;Wulff B.;Wilgus T.;Bailey J.;Supp D.;Powell H. (09-01-2017. ) Inflammatory responses, matrix remodeling, and re-epithelialization after fractional CO2&.Lasers in Surgery and Medicine, , 49 (7 ) ,675-685 More Information

Blackstone B.;Kim J.;McFarland K.;Sen C.;Supp D.;Bailey J.;Powell H. (07-01-2017. ) Scar formation following excisional and burn injuries in a red Duroc pig model.Wound Repair and Regeneration, , 25 (4 ) ,618-631 More Information

Supp D.;Boyce S. (04-19-2016. ) Bioengineering of human skin substitutes .Tissue Engineering and Artificial Organs, , 715-731

Kim J.;Dunham D.;Supp D.;Sen C.;Powell H. (03-01-2016. ) Novel burn device for rapid, reproducible burn wound generation.Burns, , 42 (2 ) ,384-391 More Information

Boyce S.;Supp D. (01-01-2016. ) Biologic Skin Substitutes.Skin Tissue Engineering and Regenerative Medicine, , 211-238 More Information

Supp D.M.;Hahn J.M.;McFarland K.L.;Combs K.A.;Lee K.S.S.;Inceoglu B.;Wan D.;Boyce S.T.;Hammock B.D. (01-01-2016. ) Soluble epoxide hydrolase inhibition and epoxyeicosatrienoic acid treatment improve vascularization .Plastic and Reconstructive Surgery - Global Open, , 4 (12 ) , More Information

Swope V.B.;Jameson J.A.;McFarland K.L.;Supp D.M.;Miller W.E.;McGraw D.W.;Patel M.A.;Nix M.A.;Millhauser G.L.;Babcock G.F.;Abdel-Malek Z.A. (01-01-2012. ) Defining MC1R regulation in human melanocytes by its agonist ?-melanocortin and antagonists agouti s.Journal of Investigative Dermatology, , 132 (9 ) ,2255-2262 More Information

Supp D. (04-01-2011. ) Skin substitutes for burn wound healing: Current and future approaches.Expert Review of Dermatology, , 6 (2 ) ,217-227 More Information

Forouzandeh F.;Jalili R.;Hartwell R.;Allan S.;Boyce S.;Supp D.;Ghahary A. (11-01-2010. ) Local expression of indoleamine 2,3-dioxygenase suppresses T-cell-mediated rejection of an engineere.Wound Repair and Regeneration, , 18 (6 ) ,614-623 More Information

Kadekaro A.L.;Leachman S.;Kavanagh R.J.;Swope V.;Cassidy P.;Supp D.;Sartor M.;Schwemberger S.;Babcock G.;Wakamatsu K.;Ito S.;Koshoffer A.;Boissy R.E.;Manga P.;Sturm R.A.;Abdel-Malek Z.A. (01-01-2010. ) Melanocortin 1 receptor genotype: An important determinant of the damage response of melanocytes to .FASEB Journal, , 24 (10 ) ,3850-3860 More Information

Kalyanaraman B.;Supp D.;Boyce S. (05-01-2008. ) Medium flow rate regulates viability and barrier function of engineered skin substitutes in perfusio.Tissue Engineering - Part A., , 14 (5 ) ,583-593 More Information

Abdel-Malek Z.;Supp D. (02-01-2008. ) ?-Defensin 3: A novel and unexpected key that unlocks the melanocortin 1 receptor.Pigment Cell and Melanoma Research, , 21 (1 ) ,7-8 More Information

Supp D.;Neely A. (02-01-2008. ) Cutaneous antimicrobial gene therapy: Engineering human skin replacements to combat wound infection.Expert Review of Dermatology, , 3 (1 ) ,73-84 More Information

Supp D.;Boyce S. (01-01-2007. ) Bioengineering of human skin substitutes .Tissue Engineering, , 275-291

Hahn, Jennifer M; Combs, Kelly A; Powell, Heather M; Supp, Dorothy M (2023. ) A Role for Vitamin D and the Vitamin D Receptor in Keloid Disorder.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , More Information

Carney, Bonnie C; Bailey, John K; Powell, Heather M; Supp, Dorothy M; Travis, Taryn E (2023. ) Scar Management and Dyschromia: A Summary Report from the 2021 American Burn Association State of the Science Meeting.Journal of burn care & research : official publication of the American Burn Association, , 44 (3 ) ,535-545 More Information

Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Powell, Heather M; Supp, Dorothy M (2022. ) Myofibroblasts Are Not Characteristic Features of Keloid Lesions.Plastic and reconstructive surgery. Global open, , 10 (11 ) ,e4680 More Information

Burmeister, David M; Supp, Dorothy M; Clark, Richard A; Tredget, Edward E; Powell, Heather M; Enkhbaatar, Perenlei; Bohannon, Julia K; Cancio, Leopoldo C; Hill, David M; Nygaard, Rachel M (2022. ) Advantages and Disadvantages of Using Small and Large Animals in Burn Research: Proceedings of the 2021 Research Special Interest Group.Journal of burn care & research : official publication of the American Burn Association, , 43 (5 ) ,1032-1041 More Information

Bergmann, Christian B; McReynolds, Cindy B; Wan, Debin; Singh, Nalin; Goetzman, Holly; Caldwell, Charles C; Supp, Dorothy M; Hammock, Bruce D (2022. ) sEH-derived metabolites of linoleic acid drive pathologic inflammation while impairing key innate immune cell function in burn injury.Proceedings of the National Academy of Sciences of the United States of America, , 119 (13 ) ,e2120691119 More Information

Supp, Dorothy M; Hahn, Jennifer M; Combs, Kelly A; McFarland, Kevin L; Powell, Heather M (2022. ) Isolation and feeder-free primary culture of four cell types from a single human skin sample.STAR protocols, , 3 (1 ) ,101172 More Information

Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Anness, Meridith C; Supp, Dorothy M (2021. ) Analysis of HOX gene expression and the effects of HOXA9 overexpression in fibroblasts derived from keloid lesions and normal skin.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 29 (5 ) ,777-791 More Information

Bergmann, Christian B; Hammock, Bruce D; Wan, Debin; Gogolla, Falk; Goetzman, Holly; Caldwell, Charles C; Supp, Dorothy M (2021. ) TPPU treatment of burned mice dampens inflammation and generation of bioactive DHET which impairs neutrophil function.Scientific reports, , 11 (1 ) ,16555 More Information

Ruiz-Torres, Sonya; Brusadelli, Marion G; Witte, David P; Wikenheiser-Brokamp, Kathryn A; Sauter, Sharon; Nelson, Adam S; Sertorio, Mathieu; Chlon, Timothy M; Lane, Adam; Mehta, Parinda A; Myers, Kasiani C; Bedard, Mary C; Pal, Bidisha; Supp, Dorothy M; Lambert, Paul F; Komurov, Kakajan; Kovacic, Melinda Butsch; Davies, Stella M; Wells, Susanne I (2021. ) Inherited DNA Repair Defects Disrupt the Structure and Function of Human Skin.Cell stem cell, , 28 (3 ) ,424-435.e6 More Information

Powell, Heather M; Supp, Dorothy M; Bailey, J Kevin; Baumann, Molly E (2021. ) Response to the Letter to the Editor: Fractional CO2 laser ablation of porcine burn scars after grafting: Is deeper better?.Burns : journal of the International Society for Burn Injuries, , 47 (2 ) ,494-495 More Information

Baumann, Molly E; DeBruler, Danielle M; Blackstone, Britani N; Coffey, Rebecca A; Boyce, Steven T; Supp, Dorothy M; Bailey, J Kevin; Powell, Heather M (2021. ) Direct comparison of reproducibility and reliability in quantitative assessments of burn scar properties.Burns : journal of the International Society for Burn Injuries, , 47 (2 ) ,466-478 More Information

Gibson, Angela L F; Carney, Bonnie C; Cuttle, Leila; Andrews, Christine J; Kowalczewski, Christine J; Liu, Aiping; Powell, Heather M; Stone, Randolph; Supp, Dorothy M; Singer, Adam J; Shupp, Jeffrey W; Stalter, Lily; Moffatt, Lauren T (2021. ) Coming to Consensus: What Defines Deep Partial Thickness Burn Injuries in Porcine Models?.Journal of burn care & research : official publication of the American Burn Association, , 42 (1 ) ,98-109 More Information

Malara, Megan M; Blackstone, Britani N; Baumann, Molly E; Bailey, J Kevin; Supp, Dorothy M; Powell, Heather M (2020. ) Cultured Epithelial Autograft Combined with Micropatterned Dermal Template Forms Rete Ridges In Vivo.Tissue engineering. Part A, , 26 (21-22 ) ,1138-1146 More Information

Stevens, Mariana L; Zhang, Zhonghua; Johansson, Elisabet; Ray, Samriddha; Jagpal, Amrita; Ruff, Brandy P; Kothari, Arjun; He, Hua; Martin, Lisa J; Ji, Hong; Wikenheiser-Brokamp, Kathryn; Weirauch, Matthew T; Supp, Dorothy M; Biagini Myers, Jocelyn M; Khurana Hershey, Gurjit K (2020. ) Disease-associated KIF3A variants alter gene methylation and expression impacting skin barrier and atopic dermatitis risk.Nature communications, , 11 (1 ) ,4092 More Information

DeBruler, Danielle M; Baumann, Molly E; Zbinden, Jacob C; Blackstone, Britani N; Bailey, John Kevin; Supp, Dorothy M; Powell, Heather M (2020. ) Improved Scar Outcomes with Increased Daily Duration of Pressure Garment Therapy.Advances in wound care, , 9 (8 ) ,453-461 More Information

Supp, Dorothy M; Hahn, Jennifer M; Lloyd, Christopher M; Combs, Kelly A; Swope, Viki B; Abdel-Malek, Zalfa; Boyce, Steven T (2020. ) Light or Dark Pigmentation of Engineered Skin Substitutes Containing Melanocytes Protects Against Ultraviolet Light-Induced DNA Damage In Vivo.Journal of burn care & research : official publication of the American Burn Association, , 41 (4 ) ,751-760 More Information

Satish, Latha; Evdokiou, Alexander; Geletu, Eleni; Hahn, Jennifer M; Supp, Dorothy M (2020. ) Pirfenidone inhibits epithelial-mesenchymal transition in keloid keratinocytes.Burns & trauma, , 8 ,tkz007 More Information

Supp, Dorothy M (2019. ) Animal Models for Studies of Keloid Scarring.Advances in wound care, , 8 (2 ) ,77-89 More Information

Supp, Dorothy M; Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Lee, Kin Sing Stephen; Inceoglu, Bora; Wan, Debin; Boyce, Steven T; Hammock, Bruce D (2016. ) Soluble Epoxide Hydrolase Inhibition and Epoxyeicosatrienoic Acid Treatment Improve Vascularization of Engineered Skin Substitutes.Plastic and reconstructive surgery. Global open, , 4 (12 ) ,e1151 More Information

Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Supp, Dorothy M (2016. ) Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-β1.Burns & trauma, , 4 (1 ) ,30 More Information

Blackstone, Britani N; Malara, Megan M; Baumann, Molly E; McFarland, Kevin L; Supp, Dorothy M; Powell, Heather M (2023. ) Laser Micropatterning Promotes Rete Ridge Formation and Enhanced Engineered Skin Strength without Increased Inflammation.Bioengineering (Basel, Switzerland), , 10 (7 ) , More Information

Cultured Skin Substitutes, Tissue Engineering, Gene Therapy, Wound Healing

Academic - University of Cincinnati College of Medicine
Department of Surgery
Cincinnati  Ohio, 45267
Phone: 5135584617
dorothy.supp@uc.edu