Dorothy M. Supp , PHD
Research Professor
Adjunct Professor, Department of Surgery, UC COM
University of Cincinnati College of Medicine
Department of Surgery
Cincinnati, Ohio 45267
Phone 5135584617
Email dorothy.supp@uc.edu
Professional Summary
Areas of Specialization - bioengineering of human skin for improved wound healing, cutaneous gene therapy, molecular analysis of abnormal scarring, in vitro models for skin cancer research
Education
Bachelor's Degree: Cornell University, College of Agriculture and Life Sciences Ithaca, New York,
Doctoral Degree: University of Cincinnati College of Medicine Cincinnati, Ohio, 1994 (Developmental Biology)
Fellowship: Childrens Hospital Research Foundation Cincinnnati, 1997 (Postdoctoral )
Fellowship: Shriners Hospitals for Children Cincinnati, OH , (Research Fellowship )
Research and Practice Interests
The purpose of my research is to enhance the performance of cultured skin substitutes for the healing of burns and other skin wounds. Cultured skin substitutes have been used at the Shriners Burns Hospital and the University of Cincinnati as an adjunctive treatment for large burn wounds. Although significant improvements over recent years have led to better clinical outcomes, cultured skin substitutes are still limited by deficiencies in anatomy and physiology compared to native skin autograft. Using the tools of molecular biology, my laboratory can genetically modify the cells used to prepare cultured skin to modulate expression of genes involved in wound healing. Genetic modification can theoretically be used to improve the composition or performance of skin substitutes, making them more similar to native skin autograft and increasing their clinical efficacy. My lab is pursuing several lines of investigation involving cultured skin substitutes. These include: ·Comparing the gene expression profiles of cultured skin and native human skin, using cDNA microarrays. This will enable us to better characterize the cultured skin substitutes and focus on specific genes and/or pathways for regulation through genetic modification. ·Identifying safe and efficient methods for genetic modification of skin cells. ·Preparing genetically modified skin substitutes and evaluating their performance in a preclinical animal model. ·Examining expression of antimicrobial peptides, such as defensins, in cultured skin grafts, to determine the feasibility of increasing resistance to microbial contamination. ·Grafting of allogeneic skin substitutes for treatment of chronic leg ulcers. ·Studying the effects of mutations in the melanocortin 1 receptor, a candidate melanoma-susceptibility gene, in melanocytes in organotypic culture, to determine if they show increased sensitivity to UV-induced DNA damage.
Positions and Work Experience
2021 - Adjunct Professor, Department of Surgery, Division of Plastic, Reconstructive, and Hand Surgery/Burn Surgery, University of Cincinnati College of Medicine,
Research Support
Grant: #R01 GM079542 Investigators:Bailey, John; Boyce, Steven; Kagan, Richard; Supp, Dorothy; Warner, Petra; Yakuboff, Kevin 09-04-2007 -08-31-2012 National Institute of General Medical Sciences Somatic Stem C ells in Engineered Human Skin Role:Collaborator $889,200.00 Active Level:Federal
Grant: #AFIRM Investigators:Boyce, Steven; Supp, Dorothy 03-10-2008 -06-30-2013 Department of the Army Medical Research and Materiel Command Armed Forces Institute For Regenerative Medicine (AFIRM) Role:Collaborator $1,224,151.00 Active Level:Federal
Grant: #TECH 09-006 Investigators:Boyce, Steven; Supp, Dorothy 08-01-2008 -06-30-2012 Ohio Department of Development Engineered Skin Substitutes Containing Mesenchymal Stem Cells Role:Collaborator $750,000.00 Active Level:State of Ohio
Grant: #R01 ES017561 Investigators:Abdelmalek, Zalfa; Kadekaro, Ana Luisa; Supp, Dorothy 07-20-2009 -04-30-2014 National Institute of Environmental Health Sciences Impact of MC 1R Functional Variants on the DNA Damage Response of Human Melanocytes Role:Collaborator $1,795,727.00 Active Level:Federal
Grant: #3 R01 GM079542-03S1 Investigators:Boyce, Steven; Supp, Dorothy 09-30-2009 -08-31-2012 National Institute of General Medical Sciences R01 GM79542 Somatic Stem Cells in Engineered Human Skin Administrative Supplement Role:Collaborator $246,973.00 Active Level:Federal
Grant: #URC Interdisciplinary Award AY2015-16 Investigators:Boyce, Steven; Supp, Dorothy; Zhang, Yuhang 05-01-2016 -04-30-2017 UC's University Research Council ENGINEERING HAIR FOLLICLE REGENERATION BY TARGETING DERMAL SHEATH CELLS Role:Collaborator $25,000.00 Active Level:Internal UC
Grant: #R21AR0173380 Investigators:Boyce, Steven; Supp, Dorothy; Zhang, Yuhang 04-01-2018 -03-31-2020 National Institute of Arthritis, Musculoskeletal and Skin Disease Reprogramming the dermal microenvironment to induce hair follicle neogenesis in engineered skin Role:Collaborator $211,043.00 Awarded Level:Federal
Grant: #VA IPA Supp Investigators:Supp, Dorothy 04-01-2020 -03-31-2021 Department of Veterans Affairs VA IPA Supp Role:PI $8,316.00 Active Level:Federal
Grant: #2020 Industry Sponsored Research Agreement Investigators:Supp, Dorothy 12-01-2020 -05-30-2021 Redx Pharma Plc Collaboration to determine efficacy of Redx Pharma proprietary agents as treatments for Keloids Role:PI $79,032.65 Awarded Level:Foreign Industry
Grant: #Shriners 72005-CIN-21 Investigators:Supp, Dorothy 01-01-2021 -12-31-2021 Shriners Hospitals for Children - Cincinnati The Role of Vitamin D and the Vitamin D Receptor in Fibrotic Wound Healing Role:PI $257,935.00 Awarded Level:Private Non-Profit
Grant: #Shriners Epidermolysis Investigators:Supp, Dorothy 01-01-2021 -12-31-2021 Shriners Hospital for Children - International Headquarters Correction of Epidermolysis Bullosa via Genome Editing and Tissue Engineering Role:PI $224,472.00 Active Level:Private Non-Profit
Grant: #Shriners 71012-CIN-20 Investigators:Supp, Dorothy 01-01-2021 -12-31-2021 Shriners Hospital for Children - International Headquarters Personalized Ablative Laser Therapy: A New Strategy for Effective Scar Reduction Role:PI $74,226.00 Active Level:Private Non-Profit
Grant: #PR221734P1 Investigators:Supp, Dorothy 09-01-2023 -08-31-2026 Department of the Army Medical Research Acquisition Activity Tissue Engineering for Cutaneous and Systemic Epidermolysis Bullosa Gene Therapy Role:PI 1558753.00 Hold Level:Federal
Grant: #R01AR077238 Investigators:Supp, Dorothy; Xie, Changchun; Zhang, Yuhang 02-04-2021 -11-30-2025 National Institute of Arthritis, Musculoskeletal and Skin Disease Multiscale modeling of an inductive hair follicle microenvironment in engineered skin substitute Role:Collaborator $364,613.00 Awarded Level:Federal
Grant: #TBD / R01AR080946 Investigators:Supp, Dorothy 08-15-2023 -06-30-2024 National Institute of Arthritis, Musculoskeletal and Skin Disease In Situ Skin Regeneration for Full Thickness Burn Injuries Role:PI 66465.77 Hold Level:Federal
Grant: #R01AR077238 Investigators:Supp, Dorothy; Xie, Changchun; Zhang, Yuhang 02-01-2021 -11-30-2025 National Institute of Arthritis, Musculoskeletal and Skin Disease Multiscale modeling of an inductive hair follicle microenvironment in engineered skin substitute Role:Collaborator $364,613.00 Awarded Level:Federal
Grant: #prepare reverse agreement_TECG20232883 Investigators:Supp, Dorothy 09-25-2023 -09-24-2024 Ohio Department of Development Development of LK 467, a tetrapeptide MC1R agonist, for sunless tanning Role:PI 120000.00 Hold Level:Ohio, State of
Grant: #R21AR078976 Investigators:Boyce, Steven; Supp, Dorothy; Zhang, Yuhang 04-12-2021 -03-31-2023 National Institute of Arthritis, Musculoskeletal and Skin Disease Reprogramming of human fibroblasts into inductive dermal papilla cells Role:Collaborator 213345.00 Awarded Level:Federal
Grant: #MC1R (R41AR083311) Investigators:Supp, Dorothy 09-22-2023 -08-14-2024 National Institute of Arthritis, Musculoskeletal and Skin Disease Vitiligo topical treatment applying a potent, highly selective MC1R agonist Role:PI 88777.00 Hold Level:Federal
Grant: #Shriners 71003 Investigators:Supp, Dorothy 01-01-2022 -12-31-2024 Shriners Hospital for Children - International Headquarters In Situ Skin Regeneration for Full Thickness Burn Injuries Role:PI 45581.00 Hold Level:Non Profit
Grant: #GR133079 Investigators:Supp, Dorothy 08-15-2023 -06-30-2028 National Institute of Arthritis, Musculoskeletal and Skin Disease In Situ Skin Regeneration for Full Thickness Burn Injuries Role:PI 61543.00 Hold Level:Federal
Grant: #R21NS130138 Investigators:Berta, Temugin; Davidson, Steve; Supp, Dorothy 09-08-2022 -08-31-2024 National Institute of Neurological Disorders and Stroke Genetic and physiological comparison of native human sensory neurons and induced pluripotent stem cells differentiated to sensory neurons Role:Collaborator 445500.00 Awarded Level:Federal
Investigators:Supp, Dorothy 10-01-2024 -01-31-2025 Procter & Gamble Digital Accelerator at UC P&G Digital Accelerator@UC: Category B; Pilot study to screen primary skin cells for genetic risk factors for atopic dermatitis Role:PI 21087.59 Hold Level:Industry
Publications
Peer Reviewed Publications
Kim, Jayne Y; Willard, James J; Supp, Dorothy M; Roy, Sashwati; Gordillo, Gayle M; Sen, Chandan K; Powell, Heather M (2015. ) Burn Scar Biomechanics Following Pressure Garment Therapy.Plastic and reconstructive surgery, , More Information
Kim, J. Y.; Dunham, D. M.; Supp, D. M.; Sen, C. K.; Powell, H. M. (2016. ) Novel burn device for rapid, reproducible burn wound generation .Plast Reconstr Surg Glob Open, , 42 (2 ) ,384-391
Alexander, J Wesley; Supp, Dorothy M (2014. ) Role of Arginine and Omega-3 Fatty Acids in Wound Healing and Infection.Advances in wound care, , 3 (11 ) ,682-690 More Information
Hahn, J. M.; McFarland, K. L.; Combs, K. A.; Supp, D. M. (2016. ) Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-á1 .Burns Trauma, , 4 (2 ) ,30
Boyce, Steven T; Zimmerman, Rachel L; Supp, Dorothy M (2014. ) Tumorigenicity Testing in Athymic Mice of Cultured Human Melanocytes for Transplantation in Engineered Skin Substitutes.Cell transplantation, , More Information
Supp, D. M.; Hahn, J. M.; McFarland, K. L.; Combs, K. A.; Lee, K. S.; Inceoglu, B.; Wan, D.; Boyce, S. T.; Hammock, B. D. (2016. ) Soluble Epoxide Hydrolase Inhibition and Epoxyeicosatrienoic Acid Treatment Improve Vascularization of Engineered Skin Substitutes .Plast Reconstr Surg Glob Open, , 4 (12 ) ,e1151
Supp, Dorothy M; Hahn, Jennifer M; McFarland, Kevin L; Glaser, Kathryn Inhibition of hyaluronan synthase 2 reduces the abnormal migration rate of keloid keratinocytes.Journal of burn care & research : official publication of the American Burn Association, , 35 (1 ) ,84-92 More Information
Hahn, J. M.; Supp, D. M. (2017. ) Abnormal expression of the vitamin D receptor in keloid scars .Acta Biomater, , 43 (7 ) ,1506-1515
Osterburg, Andrew R; Hexley, Philip; Supp, Dorothy M; Robinson, Chad T; Noel, Greg; Ogle, Cora; Boyce, Steven T; Aronow, Bruce J; Babcock, George F (2013. ) Concerns over interspecies transcriptional comparisons in mice and humans after trauma.Proceedings of the National Academy of Sciences of the United States of America, , 110 (36 ) ,E3370 More Information
DeBruler, D. M.; Blackstone, B. N.; Baumann, M. E.; McFarland, K. L.; Wulff, B. C.; Wilgus, T. A.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2017. ) Inflammatory responses, matrix remodeling, and re-epithelialization after fractional CO2 laser treatment of scars .Acta Biomater, , 49 (7 ) ,675-685
Hahn, Jennifer M; Glaser, Kathryn; McFarland, Kevin L; Aronow, Bruce J; Boyce, Steven T; Supp, Dorothy M Keloid-derived keratinocytes exhibit an abnormal gene expression profile consistent with a distinct causal role in keloid pathology.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 21 (4 ) ,530-44 More Information
Boyce, S. T.; Lloyd, C. M.; Kleiner, M. C.; Swope, V. B.; Abdel-Malek, Z.; Supp, D. M. (2017. ) Restoration of cutaneous pigmentation by transplantation to mice of isogeneic human melanocytes in dermal-epidermal engineered skin substitutes .Acta Biomater, , 30 (6 ) ,531-540
Sriwiriyanont, Penkanok; Lynch, Kaari A; McFarland, Kevin L; Supp, Dorothy M; Boyce, Steven T (2013. ) Characterization of hair follicle development in engineered skin substitutes.PloS one, , 8 (6 ) ,e65664 More Information
Blackstone, B. N.; Kim, J. Y.; McFarland, K. L.; Sen, C. K.; Supp, D. M.; Bailey, J. K.; Powell, H. M. (2017. ) Scar formation following excisional and burn injuries in a red Duroc pig model .Wound Repair Regen., , 25 (4 ) ,618-631
Sriwiriyanont, Penkanok; Lynch, Kaari A; Maier, Elizabeth A; Hahn, Jennifer M; Supp, Dorothy M; Boyce, Steven T (2012. ) Morphogenesis of chimeric hair follicles in engineered skin substitutes with human keratinocytes and murine dermal papilla cells.Experimental dermatology, , 21 (10 ) ,783-5 More Information
Sethuraman, S. A.; Gottschlich, M. M.; Supp, D. M.; Khoury, J.; Kagan, R. J. (2017. ) Vitamin D biomarkers associated with abnormal scar formation in pediatric burn patients .Acta Biomater, , 1 (1 ) ,78-87
Swope, Viki B; Jameson, Joshua A; McFarland, Kevin L; Supp, Dorothy M; Miller, William E; McGraw, Dennis W; Patel, Mira A; Nix, Matthew A; Millhauser, Glenn L; Babcock, George F; Abdel-Malek, Zalfa A (2012. ) Defining MC1R regulation in human melanocytes by its agonist ?-melanocortin and antagonists agouti signaling protein and ?-defensin 3.The Journal of investigative dermatology, , 132 (9 ) ,2255-62 More Information
DeBruler, D. M.; Zbinden, J. C.; Baumann, M. E.; Blackstone, B. N.; Malara, M. M.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2018. ) Early cessation of pressure garment therapy results in scar contraction and thickening .J Burn Care Res, , 13 (6 ) ,e0197558
Boyce, Steven T; Rice, Rachel K; Lynch, Kaari A; Supp, Andrew P; Swope, Viki B; Kagan, Richard J; Supp, Dorothy M Assessment of replication rates of human keratinocytes in engineered skin substitutes grafted to athymic mice.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 20 (4 ) ,544-51 More Information
DeBruler, D. M.; Blackstone, B. N.; McFarland, K. L.; Baumann, M. E.; Supp, D. M.; Bailey, J. K.; Powell, H. M. (2018. ) Effect of skin graft thickness on scar development in a porcine burn model .J Burn Care Res, , 13 (17 ) ,10
Supp, Dorothy M; Hahn, Jennifer M; Glaser, Kathryn; McFarland, Kevin L; Boyce, Steven T (2012. ) Deep and superficial keloid fibroblasts contribute differentially to tissue phenotype in a novel in vivo model of keloid scar.Plastic and reconstructive surgery, , 129 (6 ) ,1259-71 More Information
Bailey, J. K.; Blackstone, B. N.; DeBruler, D. M.; Kim, J. Y.; Baumann, M. E.; McFarland, K. L.; Imeokparia, F. O.; Supp, D. M.; Powell, H. M. (2018. ) Effects of early combinatorial treatment of autologous split-thickness skin grafts in red duroc pig model using pulsed dye laser and fractional CO2 laser .Acta Biomater, , 50 (1 ) ,78-87
Supp, Dorothy M (2012. ) Genomic Reprogramming and Skin-Like Maturation of Engineered Human Skin Substitutes.Advances in wound care, , 1 (2 ) ,63-68 More Information
Blackstone, B. N.; Hahn, J. M.; McFarland, K. L.; DeBruler, D. M.; Supp, D. M.; Powell, H. M. (2018. ) Inflammatory response and biomechanical properties of coaxial scaffolds for engineered skin in vitro and post-grafting .Acta Biomater, , 80 (17 ) ,247-257
Supp, Dorothy M; Glaser, Kathryn; Hahn, Jennifer M; McFarland, Kevin L; Boyce, Steven T (2012. ) LETTER TO THE EDITOR Abnormal Responses of Keloid Tissue to Wounding Identified Using In Vitro Model System. Eplasty, , 12 ,e19
Malara, M. M.; Kim, J. Y.; Clark, J. A.; Blackstone, B. N.; Ruegsegger, M. A.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2018. ) Structural, Chemical, and Mechanical Properties of Pressure Garments as a Function of Simulated Use and Repeated Laundering .J Burn Care Res, , 39 (4 ) ,562-571
McFarland, Kevin L; Glaser, Kathryn; Hahn, Jennifer M; Boyce, Steven T; Supp, Dorothy M Culture medium and cell density impact gene expression in normal skin and abnormal scar-derived fibroblasts.Journal of burn care & research : official publication of the American Burn Association, , 32 (4 ) ,498-508 More Information
Supp, D. M. (2019. ) Animal Models for Studies of Keloid Scarring .Adv Wound Care (New Rochelle), , 8 (2 ) ,77-89
Powell, Heather M; McFarland, Kevin L; Butler, David L; Supp, Dorothy M; Boyce, Steven T (2010. ) Uniaxial strain regulates morphogenesis, gene expression, and tissue strength in engineered skin.Tissue engineering. Part A, , 16 (3 ) ,1083-92 More Information
Supp, D. M.; Hahn, J. M.; Combs, K. A.; McFarland, K. L.; Schwentker, A.; Boissy, R. E.; Boyce, S. T.; Powell, H. M.; Lucky, A. W. (2019. ) Collagen VII Expression Is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Formation in Bilayer Engineered Skin Substitutes .Burns, , 28 (9-10 ) ,1242-1256
Klingenberg, Jennifer M; McFarland, Kevin L; Friedman, Aaron J; Boyce, Steven T; Aronow, Bruce J; Supp, Dorothy M (2010. ) Engineered human skin substitutes undergo large-scale genomic reprogramming and normal skin-like maturation after transplantation to athymic mice.The Journal of investigative dermatology, , 130 (2 ) ,587-601 More Information
Hahn, J. M.; Combs, K. A.; Lloyd, C. M.; McFarland, K. L.; Boyce, S. T.; Supp, D. M. (2019. ) Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting to full thickness wounds .PLoS One, , 14 (3 ) ,e0213325
Supp, Dorothy M; Gardner, Jason; Klingenberg, Jennifer M; Neely, Alice N (2009. ) Antibiotic resistance in clinical isolates of Acinetobacter baumannii, Pseudomonas aeruginosa, and Staphylococcus aureus does not impact sensitivity to human beta defensin 4.Burns : journal of the International Society for Burn Injuries, , 35 (7 ) ,949-55 More Information
DeBruler, D. M.; Baumann, M. E.; Blackstone, B. N.; Zbinden, J. C.; McFarland, K. L.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2019. ) Role of Early Application of Pressure Garments following Burn Injury and Autografting .Plast Reconstr Surg, , 143 (2 ) ,310e-321e
McFarland, Kevin L; Klingenberg, Jennifer M; Boyce, Steven T; Supp, Dorothy M Expression of genes encoding antimicrobial proteins and members of the toll-like receptor/nuclear factor-kappaB pathways in engineered human skin.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 16 (4 ) ,534-41 More Information
Malara, M. M.; Blackstone, B. N.; Baumann, M. E.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2020. ) Cultured Epithelial Autograft Combined with Micropatterned Dermal Template Forms Rete Ridges In Vivo .Tissue Eng Part A, , 26 (21-22 ) ,1138-1146
Kalyanaraman, Balaji; Supp, Dorothy M; Boyce, Steven T (2008. ) Medium flow rate regulates viability and barrier function of engineered skin substitutes in perfusion culture.Tissue engineering. Part A, , 14 (5 ) ,583-93 More Information
Baumann, M. E.; DeBruler, D. M.; Blackstone, B. N.; Coffey, R. A.; Boyce, S. T.; Supp, D. M.; Bailey, J. K.; Powell, H. M. (2020. ) Direct comparison of reproducibility and reliability in quantitative assessments of burn scar properties .Burns, , 102 (3-4 ) ,287-297
Powell, Heather M; Supp, Dorothy M; Boyce, Steven T (2008. ) Influence of electrospun collagen on wound contraction of engineered skin substitutes.Biomaterials, , 29 (7 ) ,834-43 More Information
Boyce, S. T.; Supp, D. M.; Lloyd, C. M. (2020. ) Exogenous Keratinocyte Growth Factor Is Not Required for Pigmentation of Skin Substitutes with Three Isogeneic Cell Types .Tissue Eng Part A, , 26 (3-4 ) ,214-224
Fang, Cheng-Hui; Li, Bingguo; James, J Howard; Yahya, Ayesha; Kadeer, Nijiati; Guo, Xialing; Xiao, Chun; Supp, Dorothy M; Kagan, Richard J; Hasselgren, Per-Olof; Sheriff, Sulaiman (2007. ) GSK-3beta activity is increased in skeletal muscle after burn injury in rats.American journal of physiology. Regulatory, integrative and comparative physiology, , 293 (4 ) ,R1545-51 More Information
Blackstone, B. N.; Malara, M. M.; Baumann, M. E.; McFarland, K. L.; Supp, D. M.; Powell, H. M. (2020. ) Fractional CO2 laser micropatterning of cell-seeded electrospun collagen scaffolds enables rete ridge formation in 3D engineered skin .Acta Biomater, , 102 (3-4 ) ,287-297
Supp, Dorothy M (2007. ) Gene therapy to fight infection in skin transplants.Pharmacogenomics, , 8 (5 ) ,483-6 More Information
DeBruler, D. M.; Baumann, M. E.; Zbinden, J. C.; Blackstone, B. N.; Bailey, J. K.; Supp, D. M.; Powell, H. M. (2020. ) Improved Scar Outcomes with Increased Daily Duration of Pressure Garment Therapy .Adv Wound Care (New Rochelle), , 9 (8 ) ,453-61
Smiley, Andrea K; Gardner, Jason; Klingenberg, Jennifer M; Neely, Alice N; Supp, Dorothy M Expression of human beta defensin 4 in genetically modified keratinocytes enhances antimicrobial activity.Journal of burn care & research : official publication of the American Burn Association, , 28 (1 ) ,127-32 More Information
Supp, D. M.; Hahn, J. M.; Lloyd, C. M.; Combs, K. A.; Swope, V. B.; Abdel-Malek, Z.; Boyce, S. T. (2020. ) Light or Dark Pigmentation of Engineered Skin Substitutes Containing Melanocytes Protects Against Ultraviolet Light-Induced DNA Damage In Vivo .J Burn Care Res, , 41 (4 ) ,751-760
Smiley, Andrea K; Klingenberg, Jennifer M; Boyce, Steven T; Supp, Dorothy M (2006. ) Keratin expression in cultured skin substitutes suggests that the hyperproliferative phenotype observed in vitro is normalized after grafting.Burns : journal of the International Society for Burn Injuries, , 32 (2 ) ,135-8 More Information
Satish, L.; Evdokiou, A.; Geletu, E.; Hahn, J. M.; Supp, D. M. (2020. ) Pirfenidone inhibits epithelial-mesenchymal transition in keloid keratinocytes .Burns Trauma, , 8 (4 ) ,tkz007
Smiley, Andrea K; Klingenberg, Jennifer M; Aronow, Bruce J; Boyce, Steven T; Kitzmiller, W John; Supp, Dorothy M (2005. ) Microarray analysis of gene expression in cultured skin substitutes compared with native human skin.The Journal of investigative dermatology, , 125 (6 ) ,1286-301 More Information
Hahn, J. M.; McFarland, K. L.; Combs, K. A.; Anness, M. C.; Supp, D. M. (2021. ) Analysis of HOX gene expression and the effects of HOXA9 overexpression in fibroblasts derived from keloid lesions and normal skin .Wound Repair Regen, , 29 (5 ) ,777-791
Supp, Dorothy M; Boyce, Steven T Engineered skin substitutes: practices and potentials.Clinics in dermatology, , 23 (4 ) ,403-12 More Information
Gibson, A. L. F.; Carney, B. C.; Cuttle, L.; Andrews, C. J.; Kowalczewski, C. J.; Liu, A.; Powell, H. M.; Stone, R.; Supp, D. M.; Singer, A. J.; Shupp, J. W.; Stalter, L.; Moffatt, L. T. (2021. ) Coming to Consensus: What Defines Deep Partial Thickness Burn Injuries in Porcine Models? .J Burn Care Res, , 42 (1 ) ,98-109
Supp, Andrew P; Neely, Alice N; Supp, Dorothy M; Warden, Glenn D; Boyce, Steven T Evaluation of cytotoxicity and antimicrobial activity of Acticoat Burn Dressing for management of microbial contamination in cultured skin substitutes grafted to athymic mice. The Journal of burn care & rehabilitation, , 26 (3 ) ,238-46
Ruiz-Torres, S.; Brusadelli, M. G.; Witte, D. P.; Wikenheiser-Brokamp, K. A.; Sauter, S.; Nelson, A. S.; Sertorio, M.; Chlon, T. M.; Lane, A.; Mehta, P. A.; Myers, K. C.; Bedard, M. C.; Pal, B.; Supp, D. M.; Lambert, P. F.; Komurov, K.; Kovacic, M. B.; Davies, S. M.; Wells, S. I. (2021. ) Inherited DNA Repair Defects Disrupt the Structure and Function of Human Skin .Cell Stem Cell, , 28 (3 ) ,424-435.e6
Supp, Dorothy M; Karpinski, Andrea C; Boyce, Steven T (2004. ) Expression of human beta-defensins HBD-1, HBD-2, and HBD-3 in cultured keratinocytes and skin substitutes.Burns : journal of the International Society for Burn Injuries, , 30 (7 ) ,643-8 More Information
Supp, D. M.; Hahn, J. M.; Combs, K. A.; McFarland, K. L.; Powell, H. M. (2022. ) Isolation and feeder-free primary culture of four cell types from a single human skin sample .STAR Protoc, , 3 (1 ) ,101172
Supp, Dorothy M; Karpinski, Andrea C; Boyce, Steven T Vascular endothelial growth factor overexpression increases vascularization by murine but not human endothelial cells in cultured skin substitutes grafted to athymic mice. The Journal of burn care & rehabilitation, , 25 (4 ) ,337-45
Bergmann, C. B.; McReynolds, C. B.; Wan, D.; Singh, N.; Goetzman, H.; Caldwell, C. C.; Supp, D. M.; Hammock, B. D. (2022. ) sEH-derived metabolites of linoleic acid drive pathologic inflammation while impairing key innate immune cell function in burn injury .Proc Natl Acad Sci U S A, , 119 (13 ) ,e2120691119
Supp, Dorothy M; Wilson-Landy, Kaila; Boyce, Steven T (2002. ) Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology, , 16 (8 ) ,797-804 More Information
Supp, Dorothy M; Boyce, Steven T Overexpression of vascular endothelial growth factor accelerates early vascularization and improves healing of genetically modified cultured skin substitutes. The Journal of burn care & rehabilitation, , 23 (1 ) ,10-20
Supp, D M; Supp, A P; Bell, S M; Boyce, S T (2000. ) Enhanced vascularization of cultured skin substitutes genetically modified to overexpress vascular endothelial growth factor.The Journal of investigative dermatology, , 114 (1 ) ,5-13 More Information
Supp, D M; Bell, S M; Morgan, J R; Boyce, S T Genetic modification of cultured skin substitutes by transduction of human keratinocytes and fibroblasts with platelet-derived growth factor-A. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 8 (1 ) ,26-35
Boyce S.T.;Supp D.M.;Lloyd C.M. (02-01-2020. ) Exogenous Keratinocyte Growth Factor Is Not Required for Pigmentation of Skin Substitutes with Three.Tissue Engineering - Part A, , 26 (3-4 ) ,214-224 More Information
Blackstone B.;Malara M.;Baumann M.;McFarland K.;Supp D.;Powell H.
(01-15-2020. )
Fractional CO
Supp D.M.;Hahn J.M.;Combs K.A.;McFarland K.L.;Schwentker A.;Boissy R.E.;Boyce S.T.;Powell H.M.;Lucky A.W. (09-01-2019. ) Collagen VII Expression Is Required in Both Keratinocytes and Fibroblasts for Anchoring Fibril Forma.Cell Transplantation, , 28 (9-10 ) ,1242-1256 More Information
Hahn J.M.;Combs K.A.;Lloyd C.M.;McFarland K.L.;Boyce S.T.;Supp D.M. (03-01-2019. ) Identification of Merkel cells associated with neurons in engineered skin substitutes after grafting.PLoS ONE, , 14 (3 ) , More Information
Supp D. (02-01-2019. ) Animal Models for Studies of Keloid Scarring.Advances in Wound Care, , 8 (2 ) ,77-89 More Information
Debruler D.;Baumann M.;Blackstone B.;Zbinden J.;McFarland K.;Bailey J.;Supp D.;Powell H. (02-01-2019. ) Role of Early Application of Pressure Garments following Burn Injury and Autografting.Plastic and Reconstructive Surgery, , 143 (2 ) ,310e-321e More Information
Baumann M.E.;Blackstone B.N.;Malara M.M.;Clairmonte I.A.;Supp D.M.;Bailey J.K.;Powell H.M.
(01-01-2019. )
Fractional CO
Blackstone B.;Hahn J.;McFarland K.;DeBruler D.;Supp D.;Powell H. (10-15-2018. ) Inflammatory response and biomechanical properties of coaxial scaffolds for engineered skin in vitro.Acta Biomaterialia, , 80 ,247-257 More Information
Malara M.;Kim J.;Clark J.;Blackstone B.;Ruegsegger M.;Bailey J.;Supp D.;Powell H. (06-13-2018. ) Structural, Chemical, and Mechanical Properties of Pressure Garments as a Function of Simulated Use .Journal of Burn Care and Research, , 39 (4 ) ,562-571 More Information
DeBruler D.;Blackstone B.;McFarland K.;Baumann M.;Supp D.;Bailey J.;Powell H. (06-01-2018. ) Effect of skin graft thickness on scar development in a porcine burn model.Burns, , 44 (4 ) ,917-930 More Information
DeBruler D.;Zbinden J.;Baumann M.;Blackstone B.;Malara M.;Bailey J.;Supp D.;Powell H. (06-01-2018. ) Early cessation of pressure garment therapy results in scar contraction and thickening.PLoS ONE, , 13 (6 ) , More Information
Bailey J.;Blackstone B.;DeBruler D.;Kim J.;Baumann M.;McFarland K.;Imeokparia F.;Supp D.;Powell H. (01-01-2018. ) Effects of early combinatorial treatment of autologous split-thickness skin grafts in red duroc pig .Lasers in Surgery and Medicine, , 50 (1 ) ,78-87 More Information
Hahn J.;Supp D. (11-01-2017. ) Abnormal expression of the vitamin D receptor in keloid scars.Burns, , 43 (7 ) ,1506-1515 More Information
Boyce S.;Lloyd C.;Kleiner M.;Swope V.;Abdel-Malek Z.;Supp D. (11-01-2017. ) Restoration of cutaneous pigmentation by transplantation to mice of isogeneic human melanocytes in d.Pigment Cell and Melanoma Research, , 30 (6 ) ,531-540 More Information
Lander J.;Supp D.;He H.;Martin L.;Chen X.;Weirauch M.;Boyce S.;Kopan R. (09-01-2017. ) Analysis of chromatin accessibility in human epidermis identifies putative barrier dysfunction-sensi.PLoS ONE, , 12 (9 ) , More Information
DeBruler D.;Blackstone B.;Baumann M.;McFarland K.;Wulff B.;Wilgus T.;Bailey J.;Supp D.;Powell H.
(09-01-2017. )
Inflammatory responses, matrix remodeling, and re-epithelialization after fractional CO
Blackstone B.;Kim J.;McFarland K.;Sen C.;Supp D.;Bailey J.;Powell H. (07-01-2017. ) Scar formation following excisional and burn injuries in a red Duroc pig model.Wound Repair and Regeneration, , 25 (4 ) ,618-631 More Information
Supp D.;Boyce S. (04-19-2016. ) Bioengineering of human skin substitutes .Tissue Engineering and Artificial Organs, , 715-731
Kim J.;Dunham D.;Supp D.;Sen C.;Powell H. (03-01-2016. ) Novel burn device for rapid, reproducible burn wound generation.Burns, , 42 (2 ) ,384-391 More Information
Boyce S.;Supp D. (01-01-2016. ) Biologic Skin Substitutes.Skin Tissue Engineering and Regenerative Medicine, , 211-238 More Information
Supp D.M.;Hahn J.M.;McFarland K.L.;Combs K.A.;Lee K.S.S.;Inceoglu B.;Wan D.;Boyce S.T.;Hammock B.D. (01-01-2016. ) Soluble epoxide hydrolase inhibition and epoxyeicosatrienoic acid treatment improve vascularization .Plastic and Reconstructive Surgery - Global Open, , 4 (12 ) , More Information
Swope V.B.;Jameson J.A.;McFarland K.L.;Supp D.M.;Miller W.E.;McGraw D.W.;Patel M.A.;Nix M.A.;Millhauser G.L.;Babcock G.F.;Abdel-Malek Z.A. (01-01-2012. ) Defining MC1R regulation in human melanocytes by its agonist ?-melanocortin and antagonists agouti s.Journal of Investigative Dermatology, , 132 (9 ) ,2255-2262 More Information
Supp D. (04-01-2011. ) Skin substitutes for burn wound healing: Current and future approaches.Expert Review of Dermatology, , 6 (2 ) ,217-227 More Information
Forouzandeh F.;Jalili R.;Hartwell R.;Allan S.;Boyce S.;Supp D.;Ghahary A. (11-01-2010. ) Local expression of indoleamine 2,3-dioxygenase suppresses T-cell-mediated rejection of an engineere.Wound Repair and Regeneration, , 18 (6 ) ,614-623 More Information
Kadekaro A.L.;Leachman S.;Kavanagh R.J.;Swope V.;Cassidy P.;Supp D.;Sartor M.;Schwemberger S.;Babcock G.;Wakamatsu K.;Ito S.;Koshoffer A.;Boissy R.E.;Manga P.;Sturm R.A.;Abdel-Malek Z.A. (01-01-2010. ) Melanocortin 1 receptor genotype: An important determinant of the damage response of melanocytes to .FASEB Journal, , 24 (10 ) ,3850-3860 More Information
Kalyanaraman B.;Supp D.;Boyce S. (05-01-2008. ) Medium flow rate regulates viability and barrier function of engineered skin substitutes in perfusio.Tissue Engineering - Part A., , 14 (5 ) ,583-593 More Information
Abdel-Malek Z.;Supp D. (02-01-2008. ) ?-Defensin 3: A novel and unexpected key that unlocks the melanocortin 1 receptor.Pigment Cell and Melanoma Research, , 21 (1 ) ,7-8 More Information
Supp D.;Neely A. (02-01-2008. ) Cutaneous antimicrobial gene therapy: Engineering human skin replacements to combat wound infection.Expert Review of Dermatology, , 3 (1 ) ,73-84 More Information
Supp D.;Boyce S. (01-01-2007. ) Bioengineering of human skin substitutes .Tissue Engineering, , 275-291
Hahn, Jennifer M; Combs, Kelly A; Powell, Heather M; Supp, Dorothy M (2023. ) A Role for Vitamin D and the Vitamin D Receptor in Keloid Disorder.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , More Information
Carney, Bonnie C; Bailey, John K; Powell, Heather M; Supp, Dorothy M; Travis, Taryn E (2023. ) Scar Management and Dyschromia: A Summary Report from the 2021 American Burn Association State of the Science Meeting.Journal of burn care & research : official publication of the American Burn Association, , 44 (3 ) ,535-545 More Information
Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Powell, Heather M; Supp, Dorothy M (2022. ) Myofibroblasts Are Not Characteristic Features of Keloid Lesions.Plastic and reconstructive surgery. Global open, , 10 (11 ) ,e4680 More Information
Burmeister, David M; Supp, Dorothy M; Clark, Richard A; Tredget, Edward E; Powell, Heather M; Enkhbaatar, Perenlei; Bohannon, Julia K; Cancio, Leopoldo C; Hill, David M; Nygaard, Rachel M (2022. ) Advantages and Disadvantages of Using Small and Large Animals in Burn Research: Proceedings of the 2021 Research Special Interest Group.Journal of burn care & research : official publication of the American Burn Association, , 43 (5 ) ,1032-1041 More Information
Bergmann, Christian B; McReynolds, Cindy B; Wan, Debin; Singh, Nalin; Goetzman, Holly; Caldwell, Charles C; Supp, Dorothy M; Hammock, Bruce D (2022. ) sEH-derived metabolites of linoleic acid drive pathologic inflammation while impairing key innate immune cell function in burn injury.Proceedings of the National Academy of Sciences of the United States of America, , 119 (13 ) ,e2120691119 More Information
Supp, Dorothy M; Hahn, Jennifer M; Combs, Kelly A; McFarland, Kevin L; Powell, Heather M (2022. ) Isolation and feeder-free primary culture of four cell types from a single human skin sample.STAR protocols, , 3 (1 ) ,101172 More Information
Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Anness, Meridith C; Supp, Dorothy M (2021. ) Analysis of HOX gene expression and the effects of HOXA9 overexpression in fibroblasts derived from keloid lesions and normal skin.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, , 29 (5 ) ,777-791 More Information
Bergmann, Christian B; Hammock, Bruce D; Wan, Debin; Gogolla, Falk; Goetzman, Holly; Caldwell, Charles C; Supp, Dorothy M (2021. ) TPPU treatment of burned mice dampens inflammation and generation of bioactive DHET which impairs neutrophil function.Scientific reports, , 11 (1 ) ,16555 More Information
Ruiz-Torres, Sonya; Brusadelli, Marion G; Witte, David P; Wikenheiser-Brokamp, Kathryn A; Sauter, Sharon; Nelson, Adam S; Sertorio, Mathieu; Chlon, Timothy M; Lane, Adam; Mehta, Parinda A; Myers, Kasiani C; Bedard, Mary C; Pal, Bidisha; Supp, Dorothy M; Lambert, Paul F; Komurov, Kakajan; Kovacic, Melinda Butsch; Davies, Stella M; Wells, Susanne I (2021. ) Inherited DNA Repair Defects Disrupt the Structure and Function of Human Skin.Cell stem cell, , 28 (3 ) ,424-435.e6 More Information
Powell, Heather M; Supp, Dorothy M; Bailey, J Kevin; Baumann, Molly E (2021. ) Response to the Letter to the Editor: Fractional CO2 laser ablation of porcine burn scars after grafting: Is deeper better?.Burns : journal of the International Society for Burn Injuries, , 47 (2 ) ,494-495 More Information
Baumann, Molly E; DeBruler, Danielle M; Blackstone, Britani N; Coffey, Rebecca A; Boyce, Steven T; Supp, Dorothy M; Bailey, J Kevin; Powell, Heather M (2021. ) Direct comparison of reproducibility and reliability in quantitative assessments of burn scar properties.Burns : journal of the International Society for Burn Injuries, , 47 (2 ) ,466-478 More Information
Gibson, Angela L F; Carney, Bonnie C; Cuttle, Leila; Andrews, Christine J; Kowalczewski, Christine J; Liu, Aiping; Powell, Heather M; Stone, Randolph; Supp, Dorothy M; Singer, Adam J; Shupp, Jeffrey W; Stalter, Lily; Moffatt, Lauren T (2021. ) Coming to Consensus: What Defines Deep Partial Thickness Burn Injuries in Porcine Models?.Journal of burn care & research : official publication of the American Burn Association, , 42 (1 ) ,98-109 More Information
Malara, Megan M; Blackstone, Britani N; Baumann, Molly E; Bailey, J Kevin; Supp, Dorothy M; Powell, Heather M (2020. ) Cultured Epithelial Autograft Combined with Micropatterned Dermal Template Forms Rete Ridges In Vivo.Tissue engineering. Part A, , 26 (21-22 ) ,1138-1146 More Information
Stevens, Mariana L; Zhang, Zhonghua; Johansson, Elisabet; Ray, Samriddha; Jagpal, Amrita; Ruff, Brandy P; Kothari, Arjun; He, Hua; Martin, Lisa J; Ji, Hong; Wikenheiser-Brokamp, Kathryn; Weirauch, Matthew T; Supp, Dorothy M; Biagini Myers, Jocelyn M; Khurana Hershey, Gurjit K (2020. ) Disease-associated KIF3A variants alter gene methylation and expression impacting skin barrier and atopic dermatitis risk.Nature communications, , 11 (1 ) ,4092 More Information
DeBruler, Danielle M; Baumann, Molly E; Zbinden, Jacob C; Blackstone, Britani N; Bailey, John Kevin; Supp, Dorothy M; Powell, Heather M (2020. ) Improved Scar Outcomes with Increased Daily Duration of Pressure Garment Therapy.Advances in wound care, , 9 (8 ) ,453-461 More Information
Supp, Dorothy M; Hahn, Jennifer M; Lloyd, Christopher M; Combs, Kelly A; Swope, Viki B; Abdel-Malek, Zalfa; Boyce, Steven T (2020. ) Light or Dark Pigmentation of Engineered Skin Substitutes Containing Melanocytes Protects Against Ultraviolet Light-Induced DNA Damage In Vivo.Journal of burn care & research : official publication of the American Burn Association, , 41 (4 ) ,751-760 More Information
Satish, Latha; Evdokiou, Alexander; Geletu, Eleni; Hahn, Jennifer M; Supp, Dorothy M (2020. ) Pirfenidone inhibits epithelial-mesenchymal transition in keloid keratinocytes.Burns & trauma, , 8 ,tkz007 More Information
Supp, Dorothy M (2019. ) Animal Models for Studies of Keloid Scarring.Advances in wound care, , 8 (2 ) ,77-89 More Information
Supp, Dorothy M; Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Lee, Kin Sing Stephen; Inceoglu, Bora; Wan, Debin; Boyce, Steven T; Hammock, Bruce D (2016. ) Soluble Epoxide Hydrolase Inhibition and Epoxyeicosatrienoic Acid Treatment Improve Vascularization of Engineered Skin Substitutes.Plastic and reconstructive surgery. Global open, , 4 (12 ) ,e1151 More Information
Hahn, Jennifer M; McFarland, Kevin L; Combs, Kelly A; Supp, Dorothy M (2016. ) Partial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-β1.Burns & trauma, , 4 (1 ) ,30 More Information
Blackstone, Britani N; Malara, Megan M; Baumann, Molly E; McFarland, Kevin L; Supp, Dorothy M; Powell, Heather M (2023. ) Laser Micropatterning Promotes Rete Ridge Formation and Enhanced Engineered Skin Strength without Increased Inflammation.Bioengineering (Basel, Switzerland), , 10 (7 ) , More Information
Keywords
Cultured Skin Substitutes, Tissue Engineering, Gene Therapy, Wound Healing
Contact Information
Academic - University of Cincinnati College of Medicine
Department of Surgery
Cincinnati
Ohio, 45267
Phone: 5135584617
dorothy.supp@uc.edu