Pearl Tsang

Associate Professor

Associate Professor

University of Cincinnati
Department of Chemistry
301 Clifton Court
Cincinnati, Ohio 45221-0172
Email pearl.tsang@uc.edu

Professional Summary

Pearl Tsang obtained a BA from Barnard College, followed by a PhD at the University of Pennsylvania. After her postdoctoral positions in biological NMR, Dr. Tsang joined the faculty in the Department of Chemistry at UC. Professor Tsang is one of the several biochemists in this Department and as such her general research interests entail elucidation of structure-function relationships of proteins, nucleic acids and biomolecular complexes. One particular class of proteins studied in the Tsang lab corresponds to the aminoacyl tRNA (transfer RNA) synthetaseses. These are enzymes which are crucial for correct covalent linkage of a given amino acid to its corresponding tRNA molecule; this process is also known as 'charging' of the tRNA and is essential for accurate protein translation. These investigations are conducted in order to enhance our understanding of how these proteins carry out their function since their structural and dynamic properties are relevant to their functions. The research conducted in this lab typically involves the production, purification and characterization of the proteins and peptides studied and this involves use of molecular biology and protein purification techniques. The characterization of these molecules involves biophysical and spectroscopic (primarily solution NMR) techniques.

Education

PhD: University of Pennsylvania BAD DATE

BA: Barnard College New York, NY, 1978 (Chemistry, Biochemistry concentration)

Research and Practice Interests

• Biophysical studies of proteins and nucleic acids
• Biomolecular complexes
• Biomolecular solution NMR

Positions and Work Experience

09-01-1990 -08-31-1996 Assistant Professor of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172

09-01-1996 -To Present Associate Professor of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172

Research Support

Grant: #1R15 GM60263-01 04-01-2001 -03-31-2003 National Institutes of Health $140,000

07-01-2005 -Bad Format: 20060631 University Research Council Faculty Research Support Grant $6500

Investigators:Alison Weiss, Suri Iyer and Pearl Tsang 09-01-2010 -10-31-2011 Midwest Center for Emerging Infectious Diseases NMR Studies of Shiga Toxin Role:PI 20,000.00 Active Type:Grant Level:Regional

Grant: #G-00547-01 Investigators:Tsang, Pearl 04-01-2001 -03-31-2003 National Institutes of Health Synthesis and Conformational Study of Helix Sheet Mimics Role:PI $25,000.00 Closed Level:Federal

Investigators:Pearl Tsang, Alison Weiss and Suri Iyer Midwest Center for Emerging Infectious Diseases Elucidate the molecular basis for shiga toxin binding specificity to different glycoconjugates. $20,000 Type:Grant Level:Regional

Investigators:Karin Musier-Forsyth and Pearl Tsang 07-01-2012 -06-30-2013 NIH New York Center for the Structural Biology of HIV Genome Interactions Role:PI $189,572 Pending Type:Contract Level:National

Investigators:Pearl Tsang 09-01-2014 -08-31-2015 University of Cincinnati LEAF Role:PI $3000.00 Active Type:Grant

Investigators:Pearl Tsang 02-01-2015 -01-31-2016 University Research Council Development of RNA labeling for a dual segmental labeling approach for NMR study of protein-RNA complexes Role:PI $6500.00 Denied Type:Grant Level:University

Investigators:Pearl Tsang 12-25-2014 -1/31/0201 12:00:00 AM Faculty Development Fund Purchase of NMR analysis software for Nucleic Acids Role:PI $440.00 Active Type:Grant Level:University

Investigators:Pearl Tsang 03-26-2015 -03-29-2015 University of Cincinnati LEAF Manuscript Writing Workshop 1 Type:Grant Level:College

Pearl Tsang 09/01/2014 -08/31/2015 University of Cincinnati LEAF Role:PI Grant $3000.00 Active Percent Effort:5 Level:College

Investigators:Pearl Tsang, Eddie Merino 05-01-2016 -09-30-2016

Investigators:Pearl Tsang 05-01-2016 -09-30-2016 Procter & Gamble Production of partially purified Thiol Oxidase Enzyme from one of the following organisms- Hyphomicrobium EG, Acidithiobacillus thioparus or Acidothiobacillus thiooxidans. Role:PI 24677. Completed Type:Contract

Grant: #D100547-6261410109-1 Investigators:Mark Rance 07-01-2016 -06-30-2017 University of Cincinnati College of Medicine NMR Studies of Estrogen Receptor Role:sub-contract $6500 Completed Type:Contract Level:College

Abbreviated Publications

Peer Reviewed Publications

Yiadom, K.P.A.B., Hammamieh, R., Ukpabi, N., Tsang, P. and Yang, D.C.H. "A Peptide from the extension of Lys-tRNA synthetase binds to transfer RNA and DNA". Peptides (2003), 24, 987-998.

Refaei, Mary Anne, Combs, Al, Kojetin, Douglas J, Cavanagh, John, Caperelli, Carol, Rance, Mark, Sapitro, Jennifer, & Tsang, Pearl (2011). Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy. Journal of biomolecular NMR, 49(1), 3-7.

Liu, Sheng, Howell, Michael, Melby, Joel, & Tsang, Pearl (2011). (1)H, (13)C and (15)N resonance assignment of the anticodon binding domain of human lysyl aminoacyl tRNA synthetase. Biomolecular NMR assignments.

Liu, Sheng, Decker, Aaron, Howell, Mike, Caperelli, Carol, & Tsang, Pearl (2013). (1)H, (13)C and (15)N resonance assignment of the N-terminal domain of human lysyl aminoacyl tRNA synthetase. Biomolecular NMR assignments, 7(2), 289-92.More Information

Ukpabi, N., Sharma, S., Spaller, M.R. and Tsang, P. "1H, 15N and 13C Backbone and Side Chain Assignments of the PDZ3 Domain of PSD-95 Protein". J. Biomolecular NMR (2004), 30, 111.

Sankaranarayanan, Jagadis, Bort, Lauren N, Mandel, Sarah M, Chen, Ping, Krause, Jeanette A, Brooks, Elwood E, Tsang, Pearl, & Gudmundsdottir, Anna D (2008). Orbital-Overlap control in the solid-state reactivity of beta-azido-propiophenones: selective formation of cis-azo-dimers. Organic letters, 10(5), 937-40.

Manieri, Wanda, Moore, Molly E, Soellner, Matthew B, Tsang, Pearl, & Caperelli, Carol A (2007). Human glycinamide ribonucleotide transformylase: active site mutants as mechanistic probes. Biochemistry, 46(1), 156-63.

Ukpabi, Nkoli, Sharma, Sudhir, Spaller, Mark R, & Tsang, Pearl (2004). (1)H, (15)N and (13)C backbone and side chain assignments of PSD-95 PDZ3 protein. Journal of biomolecular NMR, 30(1), 111-2.

Yiadom, Kwabena P A B, Hammamieh, Rasha, Ukpabi, Nkoli, Tsang, Pearl, & Yang, David C H (2003). A peptide from the extension of Lys-tRNA synthetase binds to transfer RNA and DNA. Peptides, 24(7), 987-98.


Anticodon-like binding of the HIV-1 tRNA-like element to human lysyl-tRNA
synthetase
, RNA, 22, pp. 1828-1835 Sheng Liu1, Roopa Comandur2, Christopher P. Jones2,3, Pearl Tsang1*, and Karin Musier-Forsyth2*
1Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221-0172,
USA
2Department of Chemistry and Biochemistry, Center for Retrovirus Research and
Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA
 

Hairpin RNA-induced conformational change of a eukaryotic-specific lysyl-tRNA synthetase extension and role of adjacent anticodon-binding domain,
Sheng Liu1Maryanne Refaei1Shuohui Liu2Aaron Decker1Jennifer M Hinerman3Andrew B. Herr3Mike Howell4Karin Musier-Forsyth2, Pearl Tsang1*

Detection of Sortase A and Identification of Its Inhibitors by Paramagnetic Nanoparticle-Assisted Nuclear Relaxation
by Anjaly N. Vijayan, Mary Anne Refaei, Rebecca N. Silva, Pearl Tsang, and Peng Zhang


 

Inhibiting NOXO1 and CYBA binding to reduce NADPH oxidase I dependent ROS damage in skin
explants.
Prasadini Senevirathnea, Alyssa Sterlinga,b, Mary Anne Refaeia, Nazanin Mokhtarpoura, Laura
Gutierrez-Rivera,c Joshua Garcia,c Pearl Tsanga, Ana Luisa Kadekarob, Edward J. Merinoa,c*

Peer Reviewed Conference/Workshop Proceedings

Contribution of the N-terminal Domain of Human Lysyl-tRNA Synthetase to tRNALys Binding

Presentations

Invited Presentations

Pearl Tsang (03-18-2009. ) “Studies of LysRS at the University of Cincinnati" .The Ohio State University, Department of Chemistry. Other Institution. . Level:State

Pearl Tsang (03-18-2008. ) “NMR Studies of HIV-1 as well as Host-cell proteins” .Transylvania University. Level:Local

Pearl Tsang (08-2005. ) Segmental Labeling Approach to the Study of Multi-Domain Proteins by Solution NMR .NSF Igert, Bozeman, MT. Level:International

Jacob Calhoun (04-10-2014. )

Poster Presentations

T. Waters (presenter) P. Tsang, C. Robinson, S. Liu, and D. Horne (03-2010. ) Investigating the tRNA binding properties of 2D protein, a truncated version of Human lysyl aminoacyl tRNA synthetase .239th National ACS , Level:National

S. Liu, J. Jordan, T. Waters, M. Howell and P. Tsang (10-2009. ) Structural studies of the tRNA binding domains of human lysyl-tRNA synthetase .RNA Rustbelt Meeting, Mt Sterling, OH . . Level:Regional

Sheng Liu, Mike Howell, Pat Reardon, (10-2010. ) Biophysical studies of the anticodon binding domain of human lysyl-tRNA synthetase .2010 RNA Rustbelt Meeting, Cleveland, OH. . Level:Regional

Mary Anne Refaei, Al Combs, Doug Kojetin, John Cavanagh, Mark Rance, Jennifer Sapitro, Carol Caperelli and Pearl Tsang (01-20-2011. ) Observing Selected Domainsin Multi-Domain Proteins via Sortase-Mediated Ligation and NMR Spectroscopy .Keystone Symposium Frontiers of NMR in Biology , Big Sky, Montana. . Level:International

Aaron, Decker, Mary Anne Refaei, Daniel Horne, Christian Robinson, Mike Howell, Pearl Tsang (10-2010. ) Functional and RNA-binding properties of the various domains of human LysRS .Cincinnati Local ACS Meeting, University of Cincinnati. . Level:Regional

Sheng Liu, Mike Howell, Pat Reardon (10-2010. ) Biophysical studies of the anticodon binding domain of human lysyl-tRNA synthetase .Cincinnati Local ACS Meeting, University of Cincinnati. . Level:Regional

Biophysical studies of RNA binding by the anticodon binding domain of human lysyl aminoacyl tRNA synthetase (09-2011. ) Biophysical studies of RNA binding by the anticodon binding domain of human lysyl aminoacyl tRNA synthetase .2011 International Symposium on Aminoacyl tRNA Synthetases, Salt Lake City, Utah. . Level:International

Aaron Decker*, Sheng Liu, Mary Anne Refaei, Mike Howell and Pearl Tsang (10-2011. ) Segmental isotopic protein labeling approach to study Protein-RNA interactions via NMR .2011 Rustbelt RNA Meeting, Dayton, OH. . Level:Regional

Aaron Decker, Amaleah Hartman, Sheng Liu, Mike Howell and Pearl Tsang (06-01-2012. ) Structure and RNA binding properties of the N-terminal domain of human LysRS enzyme .Undergraduate Research Conference, 400 TUC. . Level:University

S. Liu*, C. P. Jones, K. M.-Forsyth, M. Howell and P. Tsang (03-02-2012. ) Studies of RNA binding by the anticodon binding domain of human lysyl aminoacyl tRNA synthetase .Graduate Poster Forum, University of Cincinnati. . Level:University

Progress towards structural study of the N-terminal domain of human lysyl aminoacyl tRNA synthetase (10-12-2012. ) S. Liu*, A, Hartman, A. Decker, C. P. Jones, K. M.-Forsyth, M. Howell and P. Tsang .2012 Local Cincinnati ACS Symposium, University of Cincinnati. . Level:Regional

S. Liu*, A, Hartman, A. Decker, C. P. Jones, K. M.-Forsyth, M. Howell and P. Tsang (10-19-2012. ) Progress towards structural study of the N-terminal domain of human lysyl aminoacyl tRNA synthetase .Rustbelt RNA Society Meeting, Dayton, OH. . Level:National

Amaleah Hartman1*, Jacob Calhoun1* (04-19-2013. ) Structure and RNA binding properties of the domains of human LysRS enzyme .Poster Symposium, University of Cincinnati. . Level:University

Jacob Calhoun, Sruthi Sundaram, Mike Howell, Pearl Tsang, etc. (04-10-2014. ) NCUR,

Sruthi Sundaram UC. .

Ian Healy, Pearl Tsang and Mary Anne Refaei (04-01-2016. ) DNAzyme Cleavage of hKtRNA: Towards site specifically labeled RNA .University of Cincinnati Undergraduate Symposium, University of Cincinnati. . UC. . Level:University

Mary Anne Refaei, Eddie Merino and Pearl Tsang (02-10-2017. ) (In Press. ) Purification of Methyl Mercaptan Oxidases .Graduate Recruitment Weekend, UC. . Level:College

Maryiah Healy, Ian Healy, Maryanne Refaei and Pearl Tsang (11-17-2017. ) Use and preparation of specific and nonspecific tRNA molecules for EMSA and FA study .Cincinnati ACS, University of Cincinnati. . Level:Local

Andrew Wood, Mary Anne Refaei, Pearl Tsang (11-09-2018. ) (In Press. ) Protein and Peptide Mediated Ligation using Sortase A .Local Cincinnati American Chemical Society, University of Cincinnati. . Professional Meeting. . Level:Local

Maryanne Refaei, Al Combs, Mike Howell, Mark Rance and Pearl Tsang (11-17-2017. ) Enzymatic Ligation of ER-β Domains for NMR Study .Cincinnati ACS Poster Session, University of Cincinnati. . Level:Local

Maryanne Refaei, Al Combs, Mike Howell, Mark Rance and Pearl Tsang (03-02-2018. ) Enzymatic Ligation of ER-β Domains for NMR Study .University of Cincinnati Graduate Recruitment Weekend, University of Cincinnati. .

Maryanne Refaei, Tsitsi Niyanmere, Al Combs, Mike Howell, Mark Rance and Pearl Tsang (02-27-2020. ) NMR Study of ER-β Domains .Graduate Recruitment Weekend Poster Session, University of Cincinnati. . UC. . Level:Department

Mary Anne Refaei, Sheng Liu, and Pearl Tsang (10-23-2020. ) Human Lysyl tRNA Synthetase Interaction with Nucleic Acid Hairpins: DNA versus RNA .RNA Rustbelt Meeting, online. . Professional Meeting. . Level:National

Honors and Awards

1976 -1977 Dean's List Status:Recipient Level:College Type:Recognition

1974 -1978 New York State Regents Scholarship Type:Fellowship

Service

University of Cincinnati, Department of Chemistry (NMR Facility Committee ) Committee Chair Type:Departmental Service Level:Department 01-02-2010 -12-31-2010

(Undergraduate Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 01-02-2010 -12-31-2010

University of Cincinnati, Department of Chemistry (Safety Committee ) Committee Member Type:Departmental Service Level:Department 01-02-2010 -06-30-2010

(Senior Molecular Biomarkers Faculty Search ) Co-Chair Type:Departmental Service Level:College 01-02-2010 -12-31-2010

(Biochemistry Division ) Special Interest Group Chair Type:Departmental Service Level:Department 09-30-2010 -12-31-2010

(Departmental Service Department Undergraduate Curriculum Committee Committee Member ) Member Type:Departmental Service Level:Department 01-04-2011 -12-10-2011

(NMR Facility Liaison ) Committee Member Type:Departmental Service 01-04-2011 -12-10-2011

(Special Interest Biochemistry ) Committee Chair Type:Departmental Service Level:Department 01-04-2011 -09-24-2011

NIH (Biophysical, Biochemical and Materials Sciences Fellowship Special Emphasis ) Member Type:Grant Panel Level:National 06-27-2011 -06-30-2011

McGraw-Hill Publishing, Inc. Reviewer Type:Editorial Service Level:National 04-15-2011 -04-28-2011

International Journal of Molecular Science Reviewer Type:Editorial Service Level:International 03-16-2012 -03-23-2012

Graduate School Judge Type:University/College Service Level:University 03-02-2012 -03-02-2012

University of Cincinnati Academic Internships Other Type:Other Level:College 05-22-2012 -05-22-2012

National Institutes of Health Review Board/Panel Type:Grant Panel Level:National 11-08-2012 -11-09-2012

(Graduate Appointments & Curriculum ) Committee Member Type:Departmental Service Level:Department

Journal of Biological Chemistry Reviewer Type:Editorial Service Level:International 07-17-2013 -07-19-2013

(Safety ) Committee Member Type:Departmental Service Level:Department

(Undergraduate Curriculum Committee ) Type:Departmental Service Level:Department

(NMR Facility Liaison ) Type:Departmental Service Level:Department

Center for Imaging Research (NIH Advisory Committee ) Committee Member Type:University/College Service Level:University

Center for Imaging Research (NIH Advisory Committee ) Type:University/College Service Level:University

(Space Committee ) Committee Member Type:Departmental Service Level:Department

(Graduate Appointments and Curriculum ) Committee Member Type:Departmental Service Level:Department

Judge Type:University/College Service Level:University

Partner in Publishing Reviewer Type:Editorial Service Level:National BAD DATE -04-15-2014

Journal of Dairy Science Peer Review/Referee Type:Editorial Service Level:National BAD DATE -BAD DATE

(Safety Committee ) Committee Member Type:Departmental Service Level:Department 08-25-2014 -04-25-2015

(Graduate Appts & Curriculum ) Committee Member Type:Departmental Service Level:Department 08-25-2014 -04-25-2015

(Undergraduate Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 08-25-2014 -04-25-2015

(NMR Liaison ) Member Type:Departmental Service Level:Department 08-25-2014 -04-25-2015

(Space Committee ) Committee Member Type:Departmental Service Level:Department 08-25-2014 -04-25-2015

UC Imaging Facility (NIH Advisory Committee ) Committee Member Type:University/College Service Level:University 01-01-2015 -06-01-2015

Department of Chemistry (Undergraduate Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 01-10-2016 -04-15-2016

Chemistry (Safety Committee ) Committee Member Type:Departmental Service Level:Department 01-10-2016 -04-15-2016

(Safety Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2018 -12-31-2018

Chemistry (Graduate Appointments & Curriculum ) Committee Member Type:Departmental Service Level:Department 01-10-2016 -04-15-2016

(Undergraduate Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2018 -12-31-2018

(UC Imaging Facility NIH Advisory Committee ) Committee Member Type:University/College Service Level:University 01-10-2016 -04-15-2016

(Graduate Appointments and Curriculum ) Committee Member Type:Departmental Service Level:Department 01-01-2018 -12-31-2018

Chemistry (Chemistry Space ) Committee Member Type:Departmental Service Level:Department 01-10-2016 -04-15-2016

(Space ) Committee Member Type:Departmental Service Level:Department 01-01-2018 -12-31-2018

Faculty Development Fund (Faculty Development Fund ) Committee Member Type:University/College Service Level:College 09-29-2017 -04-06-2018

(OEPT for incoming international students ) Committee Chair Type:Departmental Service Level:Department 08-20-2018 -08-25-2018

(Chemistry Space Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2017 -12-31-2017

Chemistry (OEPT Workshop ) Coordinator Type:Departmental Service Level:Department 08-19-2019 -08-23-2019

(UC Imaging Facility NIH Advisory Committee ) Committee Member Type:University/College Service Level:University 01-01-2017 -05-31-2017

(Safety Committee ) Committee Member Type:Departmental Service Level:Department 08-26-2019 -05-01-2020

(Chemistry Graduate Appointments & Curriculum ) Committee Member Type:Departmental Service Level:Department 01-01-2017 -12-31-2017

(Space Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2019 -05-01-2020

( Chemistry Safety Committee ) Committee Member Type:Departmental Service Level:Department

(Graduate Appointments and Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2019 -12-31-2020

(Chemistry Undergraduate Curriculum ) Committee Member Type:Departmental Service Level:Department

(Undergraduate Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2019 -12-31-2020

(Ph.D. and Undergraduate Degree Assessment ) Committee Member Type:Departmental Service Level:Department

(Space Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2020 -12-31-2020

(Graduate Appointments and Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2020 -12-31-2020

(Undergraduate Curriculum Committee ) Committee Member Type:Departmental Service Level:Department 01-01-2020 -12-31-2020

(Safety ) Committee Member Type:Departmental Service Level:Department 01-01-2020 -12-31-2020

(OEPT Workshop ) Committee Chair Type:Departmental Service Level:Department 12-01-2020 -01-15-2021

Post Graduate Training and Education

01-02-1985-01-01-1987 Postdoctoral Research Associate, Solid state NMR and relaxation studies of RNA, University of California San Diego, , La Jolla, CA 92037

01-02-1987-08-31-1990 Postdoctoral Research Associate, Biological, solution NMR of antibody and antibody-complexes., The Scripps Research Institute, Department of Molecular Biology, , La Jolla, CA 92037

Keywords

Biochemistry, biophysics, biological NMR, RNA-protein complexes

Professional Affiliation

07-01-1993 -To Present: Volunteer Professor Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati

07-01-1993 -12-31-2018: Volunteer Assistant Professor Volunteer Professor Department of Molecular Genetics, Biochemistry and Microbiology University of Cincinnati University of Cincinnati,

Courses Taught

15-CHEM-611 BIOCHEMISTRY LAB Level:Undergraduate

15-CHEM-104 GOBCHEM I Level:Undergraduate

15-CHEM-105 GOBCHEM II Level:Undergraduate

15-CHEM-106 GOBCHEM III Level:Undergraduate

Introduction to Biochemistry Level:Undergraduate

15-CHEM-612 BIOCHEMISTRY LAB Level:Undergraduate

15-CHEM-106 GOBCHEM III Level:Undergraduate

Topics in Biochemistry Level:Graduate

-CHEM-3046L BIOCHEM LAB II Level:Undergraduate

-CHEM-1020 INTRO TO (BIO)CHEM Level:Undergraduate

-CHEM-1020 INTRO TO (BIO)CHEM Level:Undergraduate

Advanced Biochemistry -Proteins

Modern Topics in Biochemistry Level:Graduate

Introduction to Biochemistry Lab Level:Undergraduate

-CHEM-3046L BIOCHEM LAB II Level:Undergraduate

Modern Topics in Biochemistry Level:Graduate

-CHEM-3046L BIOCHEM LAB II Level:Undergraduate

-CHEM-1020 INTRO TO (BIO)CHEM Level:Undergraduate

Introduction to Biochemistry Lab Level:Undergraduate

Advanced Biochemistry -Proteins Level:Graduate

Topics in Modern Biochemistry Level:Graduate

-CHEM-3046L BIOCHEM LAB II Level:Undergraduate

-CHEM-2030 SURVEY BIOCHEM I Level:Undergraduate

Biochemistry Laboratory Level:Undergraduate

-CHEM-3040 INTRO BIOCHEM Level:Undergraduate

-CHEM-2031L SURVEY BIOCH LAB II Level:Undergraduate

-CHEM-3040 INTRO BIOCHEM Level:Undergraduate

Biochem Lab I Level:Undergraduate

-CHEM-2030L SURVEY BIOCH LAB I Level:Undergraduate

-CHEM-4040 MOLECULES OF LIFE Level:Undergraduate

-CHEM-3046L BIOCHEM LAB II Level:Undergraduate

Level:Undergraduate

-CHEM-4041 BIOCHEMICAL ENERGY Level:Undergraduate

Chemistry 3045L Level:Undergraduate

-CHEM-3046L BIOCHEM LAB II

-CHEM-4040 MOLECULES OF LIFE Level:Undergraduate

Special Topics in Biochemistry Level:Graduate

-CHEM-4041 BIOCHEMICAL ENERGY Level:Undergraduate

-CHEM-3046L BIOCHEM LAB II Level:Undergraduate

Chemistry 3045L Level:Undergraduate

Faculty Development Activities

NMR Studies of Shiga Toxin Submission of URC Interdisciplinary Research proposal , University Research Council University of Cincinnati Type:Other

NMR Studies of Shiga Toxin Proposal Submission to Midwest Center for Emerging Infectious Diseases (MICEID), Midwest Center for Emerging Infectious Diseases University of Cincinnati Type:Other

BAD DATE -BAD DATE Designing Flipped Classroom Content Workshop Workshop to help in the design of flipped classroom courses, CET & L 475 Langsam Type:Workshop

04-11-2013 -04-11-2013 Grant Writer's Workshop Workshop to help with the preparation of grant proposals to NIH, NSF, Grant Writers' Seminars and Workshops Vontz Center Rieveschl Auditorium Type:Workshop

06-27-2014 -06-27-2014 TopHat Introduction to TopHat Teaching Aids, Type:Seminar

SEED GRANT Proposal, UC LEAF Type:Other

11-13-2014 Grant proposal submission, University Research Council Type:Faculty Fellowship

02-2015 -12-2015 NMR Investigation of HIV-1 RNA binding to hLysRS seed grant for research software, Faculty Development Fund University of Cincinnati Type:Faculty Fellowship

03-26-2015 -03-29-2015 Manuscript Writing Workshop Workshop on how to improve writing of manuscripts, proposals, University of Cincinnati LEAF Deer Creek Lodge and Conference Center Type:Workshop

11-20-2014 -11-20-2014 Flipping the Classroom with MindTap webinar to learn about flipping classrooms, Cengage Learning online Type:Seminar

04-15-2015 -04-15-2015 UC Diversity Workshop about improving diversity in the STEM disciplines at UC, UC LEAF YWCA Type:Workshop

05-18-2015 -05-22-2015 Designing Online Courses Workshop geared towards generation of online courses, CET & L Langsam Library Type:Workshop

11-10-2015 -11-10-2015 KSRS Grant Primer Workshop covering the overall process of applying for extra-mural grant funds in the current environment., Kaplan 400-B TUC Type:Seminar

05-04-2015 -05-08-2015 Active Learning and Gateway Classes Development of active learning strategies for chemistry lecture courses., CET & L Langsam Library Type:Workshop

Proposal Submission This is a collaborative proposal involving two UC COM faculty members and myself to conduct structural studies of the estrogen receptor via crystallography, NMR and SAXS, University Research Council Type:Other

05-20-2016 -06-30-2016 Biochemistry textbook Review Review of Biochemistry textbook for Oxford University Press., Type:Other

04-19-2017 -04-19-2017 Grant Proposal Writing-Getting Started Workshop to prepare for grant proposal preparation., GWSW University of Cincinnati Type:Workshop

10-09-2017 -10-09-2017 Grant Writing Workshop Academic Research Funding Strategies, LLC AACRC (UC campus) Type:Workshop

Biochemistry textbook Review Oxford University Press Type:Other

02-02-2019 -02-27-2019 Textbook Review Review of textbook "Principles of Biochemistry" by Lehninger, et al., MacMillan Publishing Type:Other

Other Information

I served as a poster judge at the 2012 Graduate Recruitment Weekend Poster session.,

Career Path Panelist:  I served as a Panel member to advise undergraduates of careers in scientific research during a UC Internship course offered Spring, 2012.,

I served as a poster judge at the Graduate poster forum at UC.,

Undergraduate Biochemistry Equipment
During Spring, 2016, I was able to procure (from a UC medical school) several pieces of used but functional equipment  ($4500. estimated value) to update and improve the range of equipment available for the Chemistry 3045L and 3046L biochemistry laboratory courses. Equipment acquired include a gel imager (with PC), spectrophotometer, Bio-Rad Econopump chromatography system, a temperature-controlled incubator, room temperature agitator, several gel electrophoresis power supplies, Hoefer vertical gel system, six agarose gel boxes and a microcentrifuge.  in addition, we also obtained a range of consumable supplies for these lab courses such as pipet tips, kim wipes, eppendorf tubes, parafilm and miscellaneous chemicals.,

August, 2016 OEPT Workshop

In August, I co-organized the 2016 OEPT workshop with Peng Zhang. Balu Addepalli was a third faculty member who volunteered to help Peng and I out during this week-long event.  As a result of this workshop, all of the international first year students were able to serve as teaching assistants immediately.  Most of them passed their OEPT exam and only a few passed conditionally.,

Served as Poster Judge at 2017 Graduate Student Expo on 2/10/17.,

I obtained approximately $1500. in supplies and small equipment for the Biochemistry Lab courses from a lab in the University of Cincinnati College of Medicine in Spring, 2017. The items included gel electrophoresis apparatus boxes, gel power supplies, pipettors, pipet tips, microcentrifuge, vortexers. These will benefit the students taking the Chemistry 3045L and 3046L courses, as well as the introduction to biochemistry lab courses 2030L and 2031L.,

I organized and supervised the OEPT training for a dozen incoming first year international graduate students in August, 2017.  This involved recruiting existing graduate students to help with orientation and practice required to help the incoming students prepare and take their OEPT exam at the beginning of the Fall, 2017 seminar.  As a result of this weeklong workshop, all of the OEPT participants passed their OEPT examinations and were cleared to teach right away. ,

In June, 2017, I served as a biochemistry textbook reviewer for Oxford University Press. ,

Volunteer aid at Fairview School Science Fair,

I organized and supervised the OEPT training for a dozen incoming first year international graduate students in August, 2018.  This involved recruiting existing graduate students to help with orientation and practice required to help the incoming students prepare and take their OEPT exam at the beginning of the Fall, 2018 seminar.  As a result of this weeklong workshop, all of the OEPT participants passed their OEPT examinations and were cleared to teach right away.  ,

Reviewer of Biochemistry textbook for Pearson Publishing,

I organized and supervised the OEPT training for all of the Chemistry department's incoming first year, international graduate students in August, 2019.  This was done with the help of several previous graduate student volunteers.  As a result of this weeklong workshop, all of the OEPT participants passed their OEPT examinations and were cleared to teach right away.   ,

I organized and supervised the OEPT training for the Chemistry department's incoming first year, international graduate students starting in December, 2020 with the help of CGSA members.  All the workshop participants passed their OEPT exam and were not required to take any additional English as a second language courses. Hence they were able to start their TA duties without delays. ,

Major equipment repair effort was initiated in the summer of 2020 in order to resurrect one of the NMR spectrometers residing in the Chemistry department NMR facility.  This instrument was considered to be non-functional and had therefore been left idle for more than 5 years. Its condition was unknown and there were no records regarding which of its components were faulty. In the summer, we began the process of trouble-shooting this instrument, with the help of several departmental staff, especially the newly hired NMR facility manager, Alex Greenwood. Bit by bit, the main components of this instrument were tested, fixed, parts replaced to get it to a point where the computer would communicate with the instrument again.  Funds were raised from the department and personal donations in order to cover the cost of components and consultations required for its repair.  By the end of the fall semester, most of the components were made functional again.  ,

I served as a poster judge at the 2021 UnderGraduate Showcase Poster session. ,

Contact Information

Academic - University of Cincinnati
Department of Chemistry
Cincinnati  Ohio, 45221-0172
pearl.tsang@uc.edu