Xuefeng Wang

Xuefeng Wang

Associate Professor

Hoxworth Blood Center
Phone 513-558-1450
Fax 513-558-1522

Professional Summary

My laboratory is dedicated to biomedical studies with innovative biophysical and biochemical approaches. Our current research focuses on the mechanobiology and enzymology of blood cells including platelets, monocytes/macrophages and neutrophils. We develop state-of-art DNA-based biosensors to visualize and map the forces and DNase activities generated by these cells, and investigate the physiological functions of these cellular forces and membrane-bound DNases during hemostasis and innate immune response.

Professional Experience
Associate Professor, Hoxworth blood center, UC, 08/2023 – Present
Associate Professor, Iowa State University, 01/2020 – 07/2023
Assistant Professor, Iowa State University, 08/2015 – 12/2019
Postdoctoral Fellow, UIUC, 05/2010 – 07/2015
Ph.D. Purdue University, 09/2004 – 12/2009
M.S. Tsinghua University, 09/2001 – 07/2004
B.S. Tsinghua University, 09/1997 – 09/2001

Research Support

Grant: #R35GM128747 Investigators:Wang, Xuefeng 08-01-2023 -07-31-2024 National Institute of General Medical Sciences Study the role of integrin tension in cell migration, platelet contraction and invadopodium dynamics Role:PI 405000.00 Awarded Level:Federal

Grant: #026400A / ECCS-2204447 Investigators:Wang, Xuefeng 03-01-2024 -12-31-2025 National Science Foundation Platelets on Chip: Studies of Mechanobiology of Platelet-Mediated Thrombosis Enabled by Molecular Fluorescence Sensors Grafted inside Microfluidic Chips Role:PI 98415.00 Hold Level:Federal


Peer Reviewed Publications

J. Austin, Y. Tu, K. Pal and X. Wang (2023. ) Vinculin transmits high-level integrin tensions which are dispensable for focal adhesion formation .Biophysical Journal, ,

Y. Tu, K. Pal, J. Austin and X. Wang (2022. ) Filopodial adhesive force in discrete nodes revealed by integrin molecular tension imaging .Current Biology, ,

K. Pal, Y. Tu and X. Wang (2022. ) Single-molecule force imaging reveals that podosome formation requires no extracellular integrin-ligand tensions or interactions .ACS Nano, ,

Y. Tu and X. Wang (2021. ) Tracking cell migration by cellular force footprint recorded with a mechano-optical biosensor .Biosensors and Bioelectronics, ,

S. Mao, A. Sarkar, Y. Wang, C. Song, D. N. LeVine, X. Wang and L. Que (2021. ) Microfluidic chip grafted with integrin tension sensors for evaluating the effects of flowing shear stress and ROCK inhibitor on platelets .Lab on a chip, ,

K. Pal, Y. Zhao, Y. Wang and X. Wang (2021. ) Ubiquitous membrane-bound DNase activity in podosomes and invadopodia .Journal of Cell Biology, ,

Y. Tu and X. Wang (2020. ) Recent Advances in Cell Adhesive Force Microscopy .Sensors, ,

A. Sarkar, D. N. LeVine, N. Kuzmina, Y. Zhao and X. Wang (2020. ) Cell migration driven by self-generated integrin ligand gradient on ligand-labile surfaces .Current Biology, ,

Y. Zhao, K. Pal, Y. Tu and X. Wang (2020. ) Cellular force nanoscopy with 50 nm resolution based on integrin molecular tension imaging and localization .Journal of the American Chemical Society, ,

Y. Zhao, A. Sarkar and X. Wang (2020. ) Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance .Biosensors and Bioelectronics, ,

Y. Zhao, N. Wetter and X. Wang (2019. ) Imaging integrin tension and cellular force at submicron resolution with integrative tension sensor .Journal of Visualized Experiments, ,

12. Y. Wang, Y. Zhao, A. Sarkar, X. Wang (2019. ) Optical sensor revealed abnormal nuclease spatial activity on cancer cell membrane .Journal of Biophotonics, ,

Y. Zhao, Y. Wang, A. Sarkar and X. Wang (2018. ) Keratocytes Generate High Integrin Tension at the Trailing Edge to Mediate Rear De-adhesion during Rapid Cell Migration .iScience, ,

A. Sarkar, Y. Zhao, Y. Wang and X. Wang (2018. ) Force-activatable coating enables high-resolution cellular force imaging directly on regular cell culture surfaces .Physical Biology, ,

X. Wang, S. Park, L. Zeng, A. Jain and T. Ha (2018. ) Towards single-cell single-molecule pull-down .Biophysical Journal, ,

Y. Wang, D. N. LeVine, M. Gannon, Y. Zhao, A. Sarkar, B. Hoch and X. Wang (2018. ) Force-activatable biosensor enables single platelet force mapping directly by fluorescence imaging .Biosensors and Bioelectronics, ,

Y. Wang and X. Wang (2016. ) Integrins outside focal adhesions transmit tensions during stable cell adhesion .Scientific Reports, ,

X. Wang and T. Ha (2013. ) Defining Single Molecular Forces Required to Activate Integrin and Notch Signaling .Science, ,

Contact Information

Academic - Hoxworth Blood Center
Phone: 513-558-1450
Fax: 513-558-1522