Xuefeng Wang

Xuefeng Wang

Associate Professor

Hoxworth Blood Center
Phone 513-558-1450
Fax 513-558-1522

Professional Summary

My laboratory is dedicated to biomedical studies with innovative biophysical and biochemical approaches. Our current research focuses on the mechanobiology and enzymology of blood cells including platelets, monocytes/macrophages and neutrophils. We develop state-of-art DNA-based biosensors to visualize and map the forces and DNase activities generated by these cells, and investigate the physiological functions of these cellular forces and membrane-bound DNases during hemostasis and innate immune response.

Professional Experience
Associate Professor, Hoxworth blood center, UC, 08/2023 – Present
Associate Professor, Iowa State University, 01/2020 – 07/2023
Assistant Professor, Iowa State University, 08/2015 – 12/2019
Postdoctoral Fellow, UIUC, 05/2010 – 07/2015
Ph.D. Purdue University, 09/2004 – 12/2009
M.S. Tsinghua University, 09/2001 – 07/2004
B.S. Tsinghua University, 09/1997 – 09/2001

Research Support

Grant: #R35GM128747 Investigators:Wang, Xuefeng 08-01-2018 -07-31-2024 National Institute of General Medical Sciences Study the role of integrin tension in cell migration, platelet contraction and invadopodium dynamics Role:PI $1,845,555.00 Completed

Grant: #Award # 2204447 Investigators:Wang, Xuefeng 09-01-2022 -08-31-2025 National Science Foundation Platelets on Chip: Studies of Mechanobiology of Platelet-Mediated Thrombosis Enabled by Molecular Fluorescence Sensors Grafted inside Microfluidic Chips Role:Co-PI $492,076.00 Active

Grant: #Award# 1825724 Investigators:Wang, Xuefeng 09-01-2018 -08-31-2023 National Science Foundation Study of the regulative role of integrin tension in the formation and function of plasma membrane protrusions in cancer cells Role:PI $371,028.00 Completed

Grant: #2R35GM128747 Investigators:Wang, Xuefeng 01-01-2025 -12-31-2029 National Institute of General Medical Sciences Study the role of integrin tension in cell migration, platelet functions and phagocytosis Role:PI $2,316,600.00 Active

Publications

Peer Reviewed Publications

S. Kundu, K. Pal, A. Pyne and X. Wang (2025. ) Force-bearing phagocytic adhesion rings mediate the phagocytosis of surface-bound particles .Nature Communications, ,

V. Pandey, S. Kundu, A. Pyne and X. Wang (2025. ) Live-Cell Imaging of Single Integrin Tensions with Minimal Background Fluorescence Noise .Biophysical Journal, ,

Niraula G.; Pyne A.; Wang X. (07-26-2024. ) Develop Tandem Tension Sensor to Gauge Integrin-Transmitted Molecular Forces.ACS Sensors, , 9 (7 ) ,3660-3670 More Information

Sarkar A.; Niraula G.; LeVine D.; Zhao Y.; Tu Y.; Mollaeian K.; Ren J.; Que L.; Wang X. (10-27-2023. ) Development of a Ratiometric Tension Sensor Exclusively Responding to Integrin Tension Magnitude in .ACS Sensors, , 8 (10 ) ,3701-3712 More Information

Austin J.; Tu Y.; Pal K.; Wang X. (01-03-2023. ) Vinculin transmits high-level integrin tensions that are dispensable for focal adhesion formation.Biophysical Journal, , 122 (1 ) ,156-167 More Information

Tu Y.; Wang X. (01-01-2023. ) Imaging Cell Adhesive Force at the Single Molecule Level.Methods in Molecular Biology, , 2600 ,207-217 More Information

Tu Y.; Pal K.; Austin J.; Wang X. (10-24-2022. ) Filopodial adhesive force in discrete nodes revealed by integrin molecular tension imaging.Current Biology, , 32 (20 ) ,4386-4396.e3 More Information

Pal K.; Tu Y.; Wang X. (02-22-2022. ) Single-Molecule Force Imaging Reveals That Podosome Formation Requires No Extracellular Integrin-Lig.ACS Nano, , 16 (2 ) ,2481-2493 More Information

Tu Y.; Wang X. (12-01-2021. ) Tracking cell migration by cellular force footprint recorded with a mechano-optical biosensor.Biosensors and Bioelectronics, , 193 , More Information

Mao S.; Sarkar A.; Wang Y.; Song C.; LeVine D.; Wang X.; Que L. (08-21-2021. ) Microfluidic chip grafted with integrin tension sensors for evaluating the effects of flowing shear .Lab on a Chip, , 21 (16 ) ,3128-3136 More Information

Pal K.; Zhao Y.; Wang Y.; Wang X. (01-01-2021. ) Ubiquitous membrane-bound DNase activity in podosomes and invadopodia.Journal of Cell Biology, , 220 (7 ) , More Information

Tu Y.; Wang X. (12-02-2020. ) Recent advances in cell adhesive force microscopy.Sensors (Switzerland), , 20 (24 ) ,1-14 More Information

Sarkar A.; LeVine D.N.; Kuzmina N.; Zhao Y.; Wang X. (10-19-2020. ) Cell Migration Driven by Self-Generated Integrin Ligand Gradient on Ligand-Labile Surfaces.Current Biology, , 30 (20 ) ,4022-4032.e5 More Information

Zhao Y.; Pal K.; Tu Y.; Wang X. (04-15-2020. ) Cellular Force Nanoscopy with 50 nm Resolution Based on Integrin Molecular Tension Imaging and Local.Journal of the American Chemical Society, , 142 (15 ) ,6930-6934 More Information

Zhao Y.; Sarkar A.; Wang X. (02-15-2020. ) Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance.Biosensors and Bioelectronics, , 150 , More Information

Wang Y.; Zhao Y.; Sarkar A.; Wang X. (05-01-2019. ) Optical sensor revealed abnormal nuclease spatial activity on cancer cell membrane.Journal of Biophotonics, , 12 (5 ) , More Information

Zhao Y.; Wetter N.M.; Wang X. (04-01-2019. ) Imaging integrin tension and cellular force at submicron resolution with an integrative tension sens.Journal of Visualized Experiments, , 2019 (146 ) , More Information

Zhao Y.; Wang Y.; Sarkar A.; Wang X. (11-30-2018. ) Keratocytes Generate High Integrin Tension at the Trailing Edge to Mediate Rear De-adhesion during R.iScience, , 9 ,502-512 More Information

Wang X.; Park S.; Zeng L.; Jain A.; Ha T. (07-17-2018. ) Toward Single-Cell Single-Molecule Pull-Down.Biophysical Journal, , 115 (2 ) ,283-288 More Information

Sarkar A.; Zhao Y.; Wang Y.; Wang X. (06-25-2018. ) Force-activatable coating enables high-resolution cellular force imaging directly on regular cell cu.Physical Biology, , 15 (6 ) , More Information

Wang Y.; LeVine D.N.; Gannon M.; Zhao Y.; Sarkar A.; Hoch B.; Wang X. (02-15-2018. ) Force-activatable biosensor enables single platelet force mapping directly by fluorescence imaging.Biosensors and Bioelectronics, , 100 ,192-200 More Information

Wang Y.; Wang X. (11-15-2016. ) Integrins outside focal adhesions transmit tensions during stable cell adhesion.Scientific Reports, , 6 , More Information

Rahil Z.; Pedron S.; Wang X.; Ha T.; Harley B.; Leckband D. (09-01-2016. ) Nanoscale mechanics guides cellular decision making.Integrative Biology (United Kingdom), , 8 (9 ) ,929-935 More Information

Chowdhury F.; Li I.T.S.; Ngo T.T.M.; Leslie B.J.; Kim B.C.; Sokoloski J.E.; Weiland E.; Wang X.; Chemla Y.R.; Lohman T.M.; Ha T. (06-08-2016. ) Defining single molecular forces required for notch activation using nano yoyo.Nano Letters, , 16 (6 ) ,3892-3897 More Information

Wang X.; Rahil Z.; Li I.T.S.; Chowdhury F.; Leckband D.E.; Chemla Y.R.; Ha T. (02-15-2016. ) Constructing modular and universal single molecule tension sensor using protein G to study mechano-s.Scientific Reports, , 6 , More Information

Roein-Peikar M.; Xu Q.; Wang X.; Ha T. (01-01-2016. ) Ultrasensitivity of cell adhesion to the presence of mechanically strong ligands.Physical Review X, , 6 (1 ) , More Information

Lee M.K.; Park J.; Wang X.; Roein-Peikar M.; Ko E.; Qin E.; Lee J.; Ha T.; Kong H. (01-01-2016. ) Rupture force of cell adhesion ligand tethers modulates biological activities of a cell-laden hydrog.Chemical Communications, , 52 (26 ) ,4757-4760 More Information

Wang X.; Sun J.; Xu Q.; Chowdhury F.; Roein-Peikar M.; Wang Y.; Ha T. (12-01-2015. ) Integrin Molecular Tension within Motile Focal Adhesions.Biophysical Journal, , 109 (11 ) ,2259-2267 More Information

Chowdhury F.; Li I.T.S.; Leslie B.J.; Do?anay S.; Singh R.; Wang X.; Seong J.; Lee S.H.; Park S.; Wang N.; Ha T. (10-01-2015. ) Single molecular force across single integrins dictates cell spreading.Integrative Biology (United Kingdom), , 7 (10 ) ,1265-1271 More Information

Wang X.; Ha T. (01-01-2013. ) Defining single molecular forces required to activate integrin and Notch signaling.Science, , 340 (6135 ) ,991-994 More Information

Contact Information

Academic - Hoxworth Blood Center
Phone: 513-558-1450
Fax: 513-558-1522