Amanda Wasylishen

Amanda R. Wasylishen , PhD

Assistant Professor

Vontz Center for Molecular Studies
3125 Eden Ave. Rm. 2312
Cincinnati, Ohio 45267
Phone 513-558-7126
Email amanda.wasylishen@uc.edu

Education

BSc: Mount Allison University, Sackville, NB, Canada, 2003 (Chemistry; Mentor: Dr. Richard Langler)

PhD: University of Toronto, Toronto, ON, Canada, 2012 (Medical Biophysics; Mentor: Dr. Linda Penn)

Postdoc: University of Texas MD Anderson Cancer Center, Houston, TX, 2018 (Genetics; Mentor: Dr. Guillermina Lozano)

Research and Practice Interests

Pancreas biology and cancers
Neuroendocrine tumors
Epigenetics 
Histone chaperones
Transcriptional regulation
Transposable elements
Genetic models

Research Support

Investigators:Dr. Guillermina Lozano & Dr. Amanda Wasylishen 03-2019 -02-2021 Investigator Award - Neuroendocrine Tumor Research Foundation Understanding the physiologically relevant functions of Daxx Role:Co-PI Completed

Grant: #NIH/NCI R21 CA242426 Investigators:Dr. Samir Hanash 07-2020 -06-2022 National Cancer Institute Identifying actionable signatures of duodenopancreatic neuroendocrine tumors in MEN1 Role:Co-Investigator Active

Grant: #K22CA258674 Investigators:Wasylishen, Amanda 02-01-2022 -01-31-2025 National Cancer Institute The DAXX/ATRX/H3.3 axis in ERV regulation and tumor suppression Role:PI 191193.00 Awarded Level:Federal

Investigators:Amanda Wasylishen 09-2013 -08-2016 Canadian Institutes of Health Research Genetic models of pancreatic neuroendocrine tumors Role:PI Completed Type:Fellowship

Investigators:Amanda Wasylishen 07-2009 -06-2012 Canadian Breast Cancer Foundation Characterizing c-Myc deregulation in breast cancer Role:PI Completed Type:Fellowship

Publications

Peer Reviewed Publications

Kim, Michael P; Li, Xinqun; Deng, Jenying; Zhang, Yun; Dai, Bingbing; Allton, Kendra L; Hughes, Tara G; Siangco, Christian; Augustine, Jithesh J; Kang, Ya'an; McDaniel, Joy M; Xiong, Shunbin; Koay, Eugene J; McAllister, Florencia; Bristow, Christopher A; Heffernan, Timothy P; Maitra, Anirban; Liu, Bin; Barton, Michelle C; Wasylishen, Amanda R; Fleming, Jason B; Lozano, Guillermina (2021. ) Oncogenic KRAS Recruits an Expansive Transcriptional Network through Mutant p53 to Drive Pancreatic Cancer Metastasis.Cancer discovery, , 11 (8 ) ,2094-2111 More Information

Fahrmann, Johannes F; Wasylishen, Amanda R; Pieterman, Carolina R C; Irajizad, Ehsan; Vykoukal, Jody; Murage, Eunice; Wu, Ranran; Dennison, Jennifer B; Krishna, Hansini; Peterson, Christine B; Lozano, Guillermina; Zhao, Hua; Do, Kim-Anh; Halperin, Daniel M; Agarwal, Sunita K; Blau, Jenny E; Del Rivero, Jaydira; Nilubol, Naris; Walter, Mary F; Welch, James M; Weinstein, Lee S; Vriens, Menno R; van Leeuwaarde, Rachel S; van Treijen, Mark J C; Valk, Gerlof D; Perrier, Nancy D; Hanash, Samir M (2021. ) A blood-based polyamine signature associated with MEN1 duodenopancreatic neuroendocrine tumor progression.The Journal of clinical endocrinology and metabolism, , More Information

Moyer, Sydney M; Wasylishen, Amanda R; Qi, Yuan; Fowlkes, Natalie; Su, Xiaoping; Lozano, Guillermina (2020. ) p53 drives a transcriptional program that elicits a non-cell-autonomous response and alters cell state in vivo.Proceedings of the National Academy of Sciences of the United States of America, , 117 (38 ) ,23663-23673 More Information

Wasylishen, Amanda R; Sun, Chang; Moyer, Sydney M; Qi, Yuan; Chau, Gilda P; Aryal, Neeraj K; McAllister, Florencia; Kim, Michael P; Barton, Michelle C; Estrella, Jeannelyn S; Su, Xiaoping; Lozano, Guillermina (2020. ) Daxx maintains endogenous retroviral silencing and restricts cellular plasticity in vivo.Science advances, , 6 (32 ) ,eaba8415 More Information

Wasylishen, Amanda R; Sun, Chang; Chau, Gilda P; Qi, Yuan; Su, Xiaoping; Kim, Michael P; Estrella, Jeannelyn S; Lozano, Guillermina (2020. ) Men1 maintains exocrine pancreas homeostasis in response to inflammation and oncogenic stress.Proceedings of the National Academy of Sciences of the United States of America, , 117 (12 ) ,6622-6629 More Information

El Beaino, Marc; Liu, Jiayong; Wasylishen, Amanda R; Pourebrahim, Rasoul; Migut, Agata; Bessellieu, Bryan J; Huang, Ke; Lin, Patrick P (2020. ) Loss of Stag2 cooperates with EWS-FLI1 to transform murine Mesenchymal stem cells.BMC cancer, , 20 (1 ) ,3 More Information

Pant, Vinod; Xiong, Shunbin; Wasylishen, Amanda R; Larsson, Connie A; Aryal, Neeraj K; Chau, Gilda; Tailor, Ramesh C; Lozano, Guillermina (2019. ) Transient enhancement of p53 activity protects from radiation-induced gastrointestinal toxicity.Proceedings of the National Academy of Sciences of the United States of America, , 116 (35 ) ,17429-17437 More Information

Aryal, Neeraj K; Pant, Vinod; Wasylishen, Amanda R; Rimel, Bobbie J; Baseler, Laura; El-Naggar, Adel K; Mutch, David G; Goodfellow, Paul J; Arur, Swathi; Lozano, Guillermina (2019. ) Dicer1 Phosphomimetic Promotes Tumor Progression and Dissemination.Cancer research, , 79 (10 ) ,2662-2668 More Information

Samdani, Rashmi T; Wasylishen, Amanda R; Halperin, Daniel M; Dasari, Arvind; Yao, James C; Rashid, Asif; Estrella, Jeannelyn S (2019. ) Loss of Menin Expression by Immunohistochemistry in Pancreatic Neuroendocrine Tumors: Comparison Between Primary and Metastatic Tumors.Pancreas, , 48 (4 ) ,510-513 More Information

Aryal, Neeraj K; Pant, Vinod; Wasylishen, Amanda R; Parker-Thornburg, Jan; Baseler, Laura; El-Naggar, Adel K; Liu, Bin; Kalia, Awdhesh; Lozano, Guillermina; Arur, Swathi (2019. ) Constitutive Dicer1 phosphorylation accelerates metabolism and aging in vivo.Proceedings of the National Academy of Sciences of the United States of America, , 116 (3 ) ,960-969 More Information

Aryal, Neeraj K; Wasylishen, Amanda R; Lozano, Guillermina (2018. ) CRISPR/Cas9 can mediate high-efficiency off-target mutations in mice in vivo.Cell death & disease, , 9 (11 ) ,1099 More Information

Wasylishen, Amanda R; Estrella, Jeannelyn S; Pant, Vinod; Chau, Gilda P; Lozano, Guillermina (2018. ) Daxx Functions Are p53-Independent In Vivo.Molecular cancer research : MCR, , 16 (10 ) ,1523-1529 More Information

Tonnessen-Murray, Crystal; Ungerleider, Nathan A; Rao, Sonia G; Wasylishen, Amanda R; Frey, Wesley D; Jackson, James G (2018. ) p53 Mediates Vast Gene Expression Changes That Contribute to Poor Chemotherapeutic Response in a Mouse Model of Breast Cancer.Translational oncology, , 11 (4 ) ,930-940 More Information

Aryal, Neeraj K; Wasylishen, Amanda R; Pant, Vinod; Riley-Croce, Maurisa; Lozano, Guillermina (2017. ) Loss of digestive organ expansion factor (Diexf) reveals an essential role during murine embryonic development that is independent of p53.Oncotarget, , 8 (61 ) ,103996-104006 More Information

Li, Lin; Liang, Shoulei; Wasylishen, Amanda R; Zhang, Yanqin; Yang, Xueli; Zhou, Bingzheng; Shan, Luling; Han, Xiuxin; Mu, Tianyang; Wang, Guowen; Xiong, Shunbin (2016. ) PLA2G16 promotes osteosarcoma metastasis and drug resistance via the MAPK pathway.Oncotarget, , 7 (14 ) ,18021-35 More Information

Kalkat, Manpreet; Chan, Pak-Kei; Wasylishen, Amanda R; Srikumar, Tharan; Kim, Sam S; Ponzielli, Romina; Bazett-Jones, David P; Raught, Brian; Penn, Linda Z (2014. ) Identification of c-MYC SUMOylation by mass spectrometry.PloS one, , 9 (12 ) ,e115337 More Information

Xiong, Shunbin; Tu, Huolin; Kollareddy, Madhusudhan; Pant, Vinod; Li, Qin; Zhang, Yun; Jackson, James G; Suh, Young-Ah; Elizondo-Fraire, Ana C; Yang, Peirong; Chau, Gilda; Tashakori, Mehrnoosh; Wasylishen, Amanda R; Ju, Zhenlin; Solomon, Hilla; Rotter, Varda; Liu, Bin; El-Naggar, Adel K; Donehower, Lawrence A; Martinez, Luis Alfonso; Lozano, Guillermina (2014. ) Pla2g16 phospholipase mediates gain-of-function activities of mutant p53.Proceedings of the National Academy of Sciences of the United States of America, , 111 (30 ) ,11145-50 More Information

Wasylishen, A R; Kalkat, M; Kim, S S; Pandyra, A; Chan, P-K; Oliveri, S; Sedivy, E; Konforte, D; Bros, C; Raught, B; Penn, L Z (2014. ) MYC activity is negatively regulated by a C-terminal lysine cluster.Oncogene, , 33 (8 ) ,1066-72 More Information

Goard, Carolyn A; Chan-Seng-Yue, Michelle; Mullen, Peter J; Quiroga, Ariel D; Wasylishen, Amanda R; Clendening, James W; Sendorek, Dorota H S; Haider, Syed; Lehner, Richard; Boutros, Paul C; Penn, Linda Z (2014. ) Identifying molecular features that distinguish fluvastatin-sensitive breast tumor cells.Breast cancer research and treatment, , 143 (2 ) ,301-12 More Information

Wasylishen, Amanda R; Chan-Seng-Yue, Michelle; Bros, Christina; Dingar, Dharmendra; Tu, William B; Kalkat, Manpreet; Chan, Pak-Kei; Mullen, Peter J; Huang, Ling; Meyer, Natalie; Raught, Brian; Boutros, Paul C; Penn, Linda Z (2013. ) MYC phosphorylation at novel regulatory regions suppresses transforming activity.Cancer research, , 73 (21 ) ,6504-15 More Information

Wasylishen, A R; Stojanova, A; Oliveri, S; Rust, A C; Schimmer, A D; Penn, L Z (2011. ) New model systems provide insights into Myc-induced transformation.Oncogene, , 30 (34 ) ,3727-34 More Information

Luoto, Kaisa R; Meng, Alice X; Wasylishen, Amanda R; Zhao, Helen; Coackley, Carla L; Penn, Linda Z; Bristow, Robert G (2010. ) Tumor cell kill by c-MYC depletion: role of MYC-regulated genes that control DNA double-strand break repair.Cancer research, , 70 (21 ) ,8748-59 More Information

Wong, W Wei-Lynn; Boutros, Paul C; Wasylishen, Amanda R; Guckert, Kristal D; O'Brien, Erin M; Griffiths, Rebecca; Martirosyan, Anna R; Bros, Christina; Jurisica, Igor; Langler, Richard F; Penn, Linda Z (2010. ) Characterization of the apoptotic response of human leukemia cells to organosulfur compounds.BMC cancer, , 10 ,351 More Information

Beheshti Zavareh, Reza; Lau, Ken S; Hurren, Rose; Datti, Alessandro; Ashline, David J; Gronda, Marcela; Cheung, Pam; Simpson, Craig D; Liu, Wei; Wasylishen, Amanda R; Boutros, Paul C; Shi, Hui; Vengopal, Amudha; Jurisica, Igor; Penn, Linda Z; Reinhold, Vern N; Ezzat, Shereen; Wrana, Jeff; Rose, David R; Schachter, Harry; Dennis, James W; Schimmer, Aaron D (2008. ) Inhibition of the sodium/potassium ATPase impairs N-glycan expression and function.Cancer research, , 68 (16 ) ,6688-97 More Information

Caldwell, Sharon L; Langler, Richard F; Ryan, Daniel A; Wasylishen, Amanda R (2008. ) Synthesis of the first alpha-mercaptoketal and an antifungal ketodisulfide.Journal of Sulfur Chemistry , , 29 (2 ) ,163-170 More Information

Ding, Jie; Li, Xiaolong; Khan, Sabbir; Zhang, Chen; Gao, Feng; Sen, Shayak; Wasylishen, Amanda R; Zhao, Yang; Lozano, Guillermina; Koul, Dimpy; Yung, W K Alfred (2022. ) EGFR suppresses p53 function by promoting p53 binding to DNA-PKcs: a non-canonical regulatory axis between EGFR and wild-type p53 in glioblastoma.Neuro-oncology, , More Information

Invited Publications

Kalkat, Manpreet; Wasylishen, Amanda R; Kim, Sam S; Penn, Linda (2011. ) More than MAX: Discovering the Myc interactome.Cell cycle (Georgetown, Tex.), 10 (3 ) ,374-5 More Information

Wasylishen, Amanda R; Penn, Linda Z (2010. ) Myc: the beauty and the beast.Genes & cancer, 1 (6 ) ,532-41 More Information

Book Chapter

Wasylishen, Amanda R; Lozano, Guillermina (2016 ) Attenuating the p53 Pathway in Human Cancers: Many Means to the Same End. Cold Spring Harbor perspectives in medicine .

Presentations

Invited Presentations

(11-2020. ) Daxx maintains endogenous retroviral silencing and restricts cellular plasticity in vivo .Neuroendocrine Tumor Research Foundation Symposium 2020, Virtual.

(03-2020. ) Using mouse models to understand the molecular underpinnings of PanNETs .Expertise Exchange Education Program in NETs, Houston, TX.

(10-2019. ) Daxx and Atrx loss in development, tissue homeostasis, and tumorigenesis .Neuroendocrine Tumor Research Foundation Symposium 2019, Boston, MA.

Honors and Awards

2009 -2012 Doctoral Fellowship: Characterizing c-Myc deregulation in breast cancer; Canadian Breast Cancer Foundation

2013 -2016 Fellowship: Genetic models of pancreatic neuroendocrine tumors; Canadian Institutes of Health Research

2016 Dodie P. Hawn Award in Genetics Outstanding Post-Doctoral Fellow; Department of Genetics, University of Texas MD Anderson Cancer Center

2011 Travel Award; Canadian Institutes for Health Research

2002 Goodridge Undergraduate Summer Research Fellowship; Mount Allison University

Post Graduate Training and Education

2012-2018 Postdoctoral Fellow, Mentor: Dr. Guillermina Lozano, University of Texas MD Anderson Cancer Center, , Houston, TX

2018-2021 Instructor, Mentor: Dr. Guillermina Lozano, University of Texas MD Anderson Cancer Center, , Houston, TX

Contact Information

Academic - Vontz Center for Molecular Studies
3125 Eden Ave. Rm. 2312
Cincinnati  Ohio, 45267
Phone: 513-558-7126
amanda.wasylishen@uc.edu